Prevalence of hepatitis G virus and characterization of viral genome in Ghana
Identifieur interne : 000019 ( Istex/Curation ); précédent : 000018; suivant : 000020Prevalence of hepatitis G virus and characterization of viral genome in Ghana
Auteurs : Takahide Saito [Japon] ; Koh-Ichi Ishikawa [Japon] ; Mubarak Osei-Kwasi [Ghana] ; Tamiko Kaneko [Japon] ; James A. M Brandful [Ghana] ; Victor Nuvor [Ghana] ; Simeon Aidoo [Ghana] ; William Ampofo [Ghana] ; Frank A. Apeagyei [Ghana] ; Jane E. Ansah [Ghana] ; Yaw Adu-Sarkodie [Ghana] ; Francis K. Nkrumah [Ghana] ; Kenji Abe [Japon]Source :
- Hepatology Research [ 1386-6346 ] ; 1999.
Descripteurs français
- Wicri :
- topic : Recherche médicale.
English descriptors
- KwdEn :
- Accession numbers, Aids research center, Biochem biophys, Biol evol, Boehringer mannheim, Cdna, Comput appl biosci, Different ghanaian, Disease association, Elsevier science ireland, Entire nucleotide sequence, Entire sequence, Genetic heterogeneity, Genome, Genomic organization, Genotype, Ghanaian, Ghanaian individuals, Hepatitis, Hepatol commun, Hepatology, Hepatology research, High prevalence, Human virus, Human virus infection, Infection rate, Infection route, Infectious diseases, Japanese patient, Japanese patients, Liver disease, Liver diseases, Major genotypes, Medical research, National institute, Nucleotide, Nucleotide deletion, Nucleotide level, Nucleotide sequence, Passive hemagglutination method, Perkin elmer, Phylogenetic, Phylogenetic analysis, Present study, Prevalence, Primer, Risk factors, Saito, Same position, Same virus, Serum samples, Sexual transmission, Surface antigen, Terminal sequence, Viral genome, Virol, Virus genome, Virus infection, Virus infections, West africa, West africa type.
- Teeft :
- Accession numbers, Aids research center, Biochem biophys, Biol evol, Boehringer mannheim, Cdna, Comput appl biosci, Different ghanaian, Disease association, Elsevier science ireland, Entire nucleotide sequence, Entire sequence, Genetic heterogeneity, Genome, Genomic organization, Genotype, Ghanaian, Ghanaian individuals, Hepatitis, Hepatol commun, Hepatology, Hepatology research, High prevalence, Human virus, Human virus infection, Infection rate, Infection route, Infectious diseases, Japanese patient, Japanese patients, Liver disease, Liver diseases, Major genotypes, Medical research, National institute, Nucleotide, Nucleotide deletion, Nucleotide level, Nucleotide sequence, Passive hemagglutination method, Perkin elmer, Phylogenetic, Phylogenetic analysis, Present study, Prevalence, Primer, Risk factors, Saito, Same position, Same virus, Serum samples, Sexual transmission, Surface antigen, Terminal sequence, Viral genome, Virol, Virus genome, Virus infection, Virus infections, West africa, West africa type.
Abstract
The prevalence of hepatitis G virus (HGV) infection was investigated in 85 human immunodeficiency virus (HIV)-infected and 30 uninfected individuals' sera obtained from Ghanaians. HGV RNA in serum was identified by a nested reverse transcription polymerase chain reaction (RT-PCR) using primers derived from the 5′-noncoding region. We also tested for hepatitis C virus by nested RT-PCR and for hepatitis B surface antigen (HBsAg) by passive hemagglutination method. HGV RNA was detected in 17 of 85 (20%) HIV sero-positive and three of 30 (10%) sero-negative Ghanaians, respectively. The prevalence of HGV infection was much greater than hepatitis C (0.9%) and hepatitis B virus (7.8%) infections in the present study. Ninety four percent of HGV infected patients were seronegative for hepatitis B and C virus infections. The nine different Ghanaian isolates in the 5′-untranslated region of the HGV genome had one nucleotide deletion at the same position when compared with other HGV isolates. Phylogenetic analysis showed that all Ghanaian isolates belonged to type 1 (West Africa type) of the HGV genotypes. Moreover, we determined nearly full-length nucleotide sequence of the HGV genome (denoted HGV-GA128) recovered from a Ghanaian infected with HIV. The HGV-GA128 was composed of 9231 nucleotides and had a single open reading frame, encoding 2843 amino acid residues. This isolate differed from previously reported HGV/GBV-C isolates by 10–15% of the nucleotide sequence and 2–5% of the amino acid sequence. Our data indicate a high prevalence of HGV, especially genotype 1, in Ghana.
Url:
DOI: 10.1016/S1386-6346(98)00095-3
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<term>Cdna</term>
<term>Comput appl biosci</term>
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<term>Disease association</term>
<term>Elsevier science ireland</term>
<term>Entire nucleotide sequence</term>
<term>Entire sequence</term>
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<term>Hepatol commun</term>
<term>Hepatology</term>
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<term>Human virus</term>
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<term>Infection rate</term>
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<term>Hepatology</term>
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<front><div type="abstract" xml:lang="en">The prevalence of hepatitis G virus (HGV) infection was investigated in 85 human immunodeficiency virus (HIV)-infected and 30 uninfected individuals' sera obtained from Ghanaians. HGV RNA in serum was identified by a nested reverse transcription polymerase chain reaction (RT-PCR) using primers derived from the 5′-noncoding region. We also tested for hepatitis C virus by nested RT-PCR and for hepatitis B surface antigen (HBsAg) by passive hemagglutination method. HGV RNA was detected in 17 of 85 (20%) HIV sero-positive and three of 30 (10%) sero-negative Ghanaians, respectively. The prevalence of HGV infection was much greater than hepatitis C (0.9%) and hepatitis B virus (7.8%) infections in the present study. Ninety four percent of HGV infected patients were seronegative for hepatitis B and C virus infections. The nine different Ghanaian isolates in the 5′-untranslated region of the HGV genome had one nucleotide deletion at the same position when compared with other HGV isolates. Phylogenetic analysis showed that all Ghanaian isolates belonged to type 1 (West Africa type) of the HGV genotypes. Moreover, we determined nearly full-length nucleotide sequence of the HGV genome (denoted HGV-GA128) recovered from a Ghanaian infected with HIV. The HGV-GA128 was composed of 9231 nucleotides and had a single open reading frame, encoding 2843 amino acid residues. This isolate differed from previously reported HGV/GBV-C isolates by 10–15% of the nucleotide sequence and 2–5% of the amino acid sequence. Our data indicate a high prevalence of HGV, especially genotype 1, in Ghana.</div>
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