Prevalence of hepatitis G virus and characterization of viral genome in Ghana
Identifieur interne : 000019 ( Istex/Corpus ); précédent : 000018; suivant : 000020Prevalence of hepatitis G virus and characterization of viral genome in Ghana
Auteurs : Takahide Saito ; Koh-Ichi Ishikawa ; Mubarak Osei-Kwasi ; Tamiko Kaneko ; James A. M Brandful ; Victor Nuvor ; Simeon Aidoo ; William Ampofo ; Frank A. Apeagyei ; Jane E. Ansah ; Yaw Adu-Sarkodie ; Francis K. Nkrumah ; Kenji AbeSource :
- Hepatology Research [ 1386-6346 ] ; 1999.
English descriptors
- KwdEn :
- Accession numbers, Aids research center, Biochem biophys, Biol evol, Boehringer mannheim, Cdna, Comput appl biosci, Different ghanaian, Disease association, Elsevier science ireland, Entire nucleotide sequence, Entire sequence, Genetic heterogeneity, Genome, Genomic organization, Genotype, Ghanaian, Ghanaian individuals, Hepatitis, Hepatol commun, Hepatology, Hepatology research, High prevalence, Human virus, Human virus infection, Infection rate, Infection route, Infectious diseases, Japanese patient, Japanese patients, Liver disease, Liver diseases, Major genotypes, Medical research, National institute, Nucleotide, Nucleotide deletion, Nucleotide level, Nucleotide sequence, Passive hemagglutination method, Perkin elmer, Phylogenetic, Phylogenetic analysis, Present study, Prevalence, Primer, Risk factors, Saito, Same position, Same virus, Serum samples, Sexual transmission, Surface antigen, Terminal sequence, Viral genome, Virol, Virus genome, Virus infection, Virus infections, West africa, West africa type.
- Teeft :
- Accession numbers, Aids research center, Biochem biophys, Biol evol, Boehringer mannheim, Cdna, Comput appl biosci, Different ghanaian, Disease association, Elsevier science ireland, Entire nucleotide sequence, Entire sequence, Genetic heterogeneity, Genome, Genomic organization, Genotype, Ghanaian, Ghanaian individuals, Hepatitis, Hepatol commun, Hepatology, Hepatology research, High prevalence, Human virus, Human virus infection, Infection rate, Infection route, Infectious diseases, Japanese patient, Japanese patients, Liver disease, Liver diseases, Major genotypes, Medical research, National institute, Nucleotide, Nucleotide deletion, Nucleotide level, Nucleotide sequence, Passive hemagglutination method, Perkin elmer, Phylogenetic, Phylogenetic analysis, Present study, Prevalence, Primer, Risk factors, Saito, Same position, Same virus, Serum samples, Sexual transmission, Surface antigen, Terminal sequence, Viral genome, Virol, Virus genome, Virus infection, Virus infections, West africa, West africa type.
Abstract
The prevalence of hepatitis G virus (HGV) infection was investigated in 85 human immunodeficiency virus (HIV)-infected and 30 uninfected individuals' sera obtained from Ghanaians. HGV RNA in serum was identified by a nested reverse transcription polymerase chain reaction (RT-PCR) using primers derived from the 5′-noncoding region. We also tested for hepatitis C virus by nested RT-PCR and for hepatitis B surface antigen (HBsAg) by passive hemagglutination method. HGV RNA was detected in 17 of 85 (20%) HIV sero-positive and three of 30 (10%) sero-negative Ghanaians, respectively. The prevalence of HGV infection was much greater than hepatitis C (0.9%) and hepatitis B virus (7.8%) infections in the present study. Ninety four percent of HGV infected patients were seronegative for hepatitis B and C virus infections. The nine different Ghanaian isolates in the 5′-untranslated region of the HGV genome had one nucleotide deletion at the same position when compared with other HGV isolates. Phylogenetic analysis showed that all Ghanaian isolates belonged to type 1 (West Africa type) of the HGV genotypes. Moreover, we determined nearly full-length nucleotide sequence of the HGV genome (denoted HGV-GA128) recovered from a Ghanaian infected with HIV. The HGV-GA128 was composed of 9231 nucleotides and had a single open reading frame, encoding 2843 amino acid residues. This isolate differed from previously reported HGV/GBV-C isolates by 10–15% of the nucleotide sequence and 2–5% of the amino acid sequence. Our data indicate a high prevalence of HGV, especially genotype 1, in Ghana.
Url:
DOI: 10.1016/S1386-6346(98)00095-3
Links to Exploration step
ISTEX:73A2BA2DCE7C630A37FEEE65A5CC15D5E68DD821Le document en format XML
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M" last="Brandful">James A. M Brandful</name><affiliation><mods:affiliation>The Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Ghana</mods:affiliation></affiliation></author><author><name sortKey="Nuvor, Victor" sort="Nuvor, Victor" uniqKey="Nuvor V" first="Victor" last="Nuvor">Victor Nuvor</name><affiliation><mods:affiliation>The Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Ghana</mods:affiliation></affiliation></author><author><name sortKey="Aidoo, Simeon" sort="Aidoo, Simeon" uniqKey="Aidoo S" first="Simeon" last="Aidoo">Simeon Aidoo</name><affiliation><mods:affiliation>The Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Ghana</mods:affiliation></affiliation></author><author><name sortKey="Ampofo, William" sort="Ampofo, William" uniqKey="Ampofo W" first="William" last="Ampofo">William Ampofo</name><affiliation><mods:affiliation>The Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Ghana</mods:affiliation></affiliation></author><author><name sortKey="Apeagyei, Frank A" sort="Apeagyei, Frank A" uniqKey="Apeagyei F" first="Frank A" last="Apeagyei">Frank A. Apeagyei</name><affiliation><mods:affiliation>Army Health Unit, 37 Military Hospital, Accra, Ghana</mods:affiliation></affiliation></author><author><name sortKey="Ansah, Jane E" sort="Ansah, Jane E" uniqKey="Ansah J" first="Jane E" last="Ansah">Jane E. Ansah</name><affiliation><mods:affiliation>Army Health Unit, 37 Military Hospital, Accra, Ghana</mods:affiliation></affiliation></author><author><name sortKey="Adu Sarkodie, Yaw" sort="Adu Sarkodie, Yaw" uniqKey="Adu Sarkodie Y" first="Yaw" last="Adu-Sarkodie">Yaw Adu-Sarkodie</name><affiliation><mods:affiliation>Department of Microbiology, Komfo Anokye Teaching Hospital, Kumasi, Ghana</mods:affiliation></affiliation></author><author><name sortKey="Nkrumah, Francis K" sort="Nkrumah, Francis K" uniqKey="Nkrumah F" first="Francis K" last="Nkrumah">Francis K. Nkrumah</name><affiliation><mods:affiliation>The Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Ghana</mods:affiliation></affiliation></author><author><name sortKey="Abe, Kenji" sort="Abe, Kenji" uniqKey="Abe K" first="Kenji" last="Abe">Kenji Abe</name><affiliation><mods:affiliation>Department of Pathology, National Institute of Infectious Diseases, Toyama 1-23-1, Shinjuku-ku, Tokyo 162-8640, Japan</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Hepatology Research</title><title level="j" type="abbrev">HEPC</title><idno type="ISSN">1386-6346</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1999">1999</date><biblScope unit="volume">13</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="221">221</biblScope><biblScope unit="page" to="231">231</biblScope></imprint><idno type="ISSN">1386-6346</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">1386-6346</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Accession numbers</term><term>Aids research center</term><term>Biochem biophys</term><term>Biol evol</term><term>Boehringer mannheim</term><term>Cdna</term><term>Comput appl biosci</term><term>Different ghanaian</term><term>Disease association</term><term>Elsevier science ireland</term><term>Entire nucleotide sequence</term><term>Entire sequence</term><term>Genetic heterogeneity</term><term>Genome</term><term>Genomic organization</term><term>Genotype</term><term>Ghanaian</term><term>Ghanaian individuals</term><term>Hepatitis</term><term>Hepatol commun</term><term>Hepatology</term><term>Hepatology research</term><term>High prevalence</term><term>Human virus</term><term>Human virus infection</term><term>Infection rate</term><term>Infection route</term><term>Infectious diseases</term><term>Japanese patient</term><term>Japanese patients</term><term>Liver disease</term><term>Liver diseases</term><term>Major genotypes</term><term>Medical research</term><term>National institute</term><term>Nucleotide</term><term>Nucleotide deletion</term><term>Nucleotide level</term><term>Nucleotide sequence</term><term>Passive hemagglutination method</term><term>Perkin elmer</term><term>Phylogenetic</term><term>Phylogenetic analysis</term><term>Present study</term><term>Prevalence</term><term>Primer</term><term>Risk factors</term><term>Saito</term><term>Same position</term><term>Same virus</term><term>Serum samples</term><term>Sexual transmission</term><term>Surface antigen</term><term>Terminal sequence</term><term>Viral genome</term><term>Virol</term><term>Virus genome</term><term>Virus infection</term><term>Virus infections</term><term>West africa</term><term>West africa type</term></keywords><keywords scheme="Teeft" xml:lang="en"><term>Accession numbers</term><term>Aids research center</term><term>Biochem biophys</term><term>Biol evol</term><term>Boehringer mannheim</term><term>Cdna</term><term>Comput appl biosci</term><term>Different ghanaian</term><term>Disease association</term><term>Elsevier science ireland</term><term>Entire nucleotide sequence</term><term>Entire sequence</term><term>Genetic heterogeneity</term><term>Genome</term><term>Genomic organization</term><term>Genotype</term><term>Ghanaian</term><term>Ghanaian individuals</term><term>Hepatitis</term><term>Hepatol commun</term><term>Hepatology</term><term>Hepatology research</term><term>High prevalence</term><term>Human virus</term><term>Human virus infection</term><term>Infection rate</term><term>Infection route</term><term>Infectious diseases</term><term>Japanese patient</term><term>Japanese patients</term><term>Liver disease</term><term>Liver diseases</term><term>Major genotypes</term><term>Medical research</term><term>National institute</term><term>Nucleotide</term><term>Nucleotide deletion</term><term>Nucleotide level</term><term>Nucleotide sequence</term><term>Passive hemagglutination method</term><term>Perkin elmer</term><term>Phylogenetic</term><term>Phylogenetic analysis</term><term>Present study</term><term>Prevalence</term><term>Primer</term><term>Risk factors</term><term>Saito</term><term>Same position</term><term>Same virus</term><term>Serum samples</term><term>Sexual transmission</term><term>Surface antigen</term><term>Terminal sequence</term><term>Viral genome</term><term>Virol</term><term>Virus genome</term><term>Virus infection</term><term>Virus infections</term><term>West africa</term><term>West africa type</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The prevalence of hepatitis G virus (HGV) infection was investigated in 85 human immunodeficiency virus (HIV)-infected and 30 uninfected individuals' sera obtained from Ghanaians. HGV RNA in serum was identified by a nested reverse transcription polymerase chain reaction (RT-PCR) using primers derived from the 5′-noncoding region. We also tested for hepatitis C virus by nested RT-PCR and for hepatitis B surface antigen (HBsAg) by passive hemagglutination method. HGV RNA was detected in 17 of 85 (20%) HIV sero-positive and three of 30 (10%) sero-negative Ghanaians, respectively. The prevalence of HGV infection was much greater than hepatitis C (0.9%) and hepatitis B virus (7.8%) infections in the present study. Ninety four percent of HGV infected patients were seronegative for hepatitis B and C virus infections. The nine different Ghanaian isolates in the 5′-untranslated region of the HGV genome had one nucleotide deletion at the same position when compared with other HGV isolates. Phylogenetic analysis showed that all Ghanaian isolates belonged to type 1 (West Africa type) of the HGV genotypes. Moreover, we determined nearly full-length nucleotide sequence of the HGV genome (denoted HGV-GA128) recovered from a Ghanaian infected with HIV. The HGV-GA128 was composed of 9231 nucleotides and had a single open reading frame, encoding 2843 amino acid residues. This isolate differed from previously reported HGV/GBV-C isolates by 10–15% of the nucleotide sequence and 2–5% of the amino acid sequence. Our data indicate a high prevalence of HGV, especially genotype 1, in Ghana.</div></front></TEI><istex><corpusName>elsevier</corpusName><keywords><teeft><json:string>genome</json:string><json:string>ghanaian</json:string><json:string>genotype</json:string><json:string>hepatology</json:string><json:string>saito</json:string><json:string>hepatology research</json:string><json:string>phylogenetic</json:string><json:string>primer</json:string><json:string>cdna</json:string><json:string>nucleotide sequence</json:string><json:string>virol</json:string><json:string>phylogenetic analysis</json:string><json:string>hepatitis</json:string><json:string>nucleotide</json:string><json:string>virus infection</json:string><json:string>present study</json:string><json:string>infectious diseases</json:string><json:string>liver diseases</json:string><json:string>high prevalence</json:string><json:string>major genotypes</json:string><json:string>west africa type</json:string><json:string>national institute</json:string><json:string>sexual transmission</json:string><json:string>virus genome</json:string><json:string>liver disease</json:string><json:string>genomic organization</json:string><json:string>terminal sequence</json:string><json:string>genetic heterogeneity</json:string><json:string>elsevier science ireland</json:string><json:string>nucleotide level</json:string><json:string>passive hemagglutination method</json:string><json:string>virus infections</json:string><json:string>viral genome</json:string><json:string>aids research center</json:string><json:string>serum samples</json:string><json:string>different ghanaian</json:string><json:string>boehringer mannheim</json:string><json:string>perkin elmer</json:string><json:string>nucleotide deletion</json:string><json:string>accession numbers</json:string><json:string>west africa</json:string><json:string>ghanaian individuals</json:string><json:string>same position</json:string><json:string>medical research</json:string><json:string>infection rate</json:string><json:string>japanese patients</json:string><json:string>infection route</json:string><json:string>human virus</json:string><json:string>entire nucleotide sequence</json:string><json:string>entire sequence</json:string><json:string>disease association</json:string><json:string>human virus infection</json:string><json:string>surface antigen</json:string><json:string>hepatol commun</json:string><json:string>risk factors</json:string><json:string>japanese patient</json:string><json:string>biochem biophys</json:string><json:string>biol evol</json:string><json:string>comput appl biosci</json:string><json:string>same virus</json:string><json:string>prevalence</json:string></teeft></keywords><author><json:item><name>Takahide Saito</name><affiliations><json:string>Department of Pathology, National Institute of Infectious Diseases, Toyama 1-23-1, Shinjuku-ku, Tokyo 162-8640, Japan</json:string></affiliations></json:item><json:item><name>Koh-ichi Ishikawa</name><affiliations><json:string>AIDS Research Center, National Institute of Infectious Diseases, Toyama 1-23-1, Shinjuku-ku, Tokyo 162-8640, Japan</json:string></affiliations></json:item><json:item><name>Mubarak Osei-Kwasi</name><affiliations><json:string>The Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Ghana</json:string></affiliations></json:item><json:item><name>Tamiko Kaneko</name><affiliations><json:string>Department of Pathology, National Institute of Infectious Diseases, Toyama 1-23-1, Shinjuku-ku, Tokyo 162-8640, Japan</json:string></affiliations></json:item><json:item><name>James A.M Brandful</name><affiliations><json:string>The Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Ghana</json:string></affiliations></json:item><json:item><name>Victor Nuvor</name><affiliations><json:string>The Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Ghana</json:string></affiliations></json:item><json:item><name>Simeon Aidoo</name><affiliations><json:string>The Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Ghana</json:string></affiliations></json:item><json:item><name>William Ampofo</name><affiliations><json:string>The Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Ghana</json:string></affiliations></json:item><json:item><name>Frank A Apeagyei</name><affiliations><json:string>Army Health Unit, 37 Military Hospital, Accra, Ghana</json:string></affiliations></json:item><json:item><name>Jane E Ansah</name><affiliations><json:string>Army Health Unit, 37 Military Hospital, Accra, Ghana</json:string></affiliations></json:item><json:item><name>Yaw Adu-Sarkodie</name><affiliations><json:string>Department of Microbiology, Komfo Anokye Teaching Hospital, Kumasi, Ghana</json:string></affiliations></json:item><json:item><name>Francis K Nkrumah</name><affiliations><json:string>The Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Ghana</json:string></affiliations></json:item><json:item><name>Kenji Abe</name><affiliations><json:string>Department of Pathology, National Institute of Infectious Diseases, Toyama 1-23-1, Shinjuku-ku, Tokyo 162-8640, Japan</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>Full-length sequence of Ghanaian HGV isolate (HGV-GA128)</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Ghana</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Hepatitis G virus (HGV) genome</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>HGV genotypes</value></json:item></subject><language><json:string>eng</json:string></language><originalGenre><json:string>Full-length article</json:string></originalGenre><abstract>The prevalence of hepatitis G virus (HGV) infection was investigated in 85 human immunodeficiency virus (HIV)-infected and 30 uninfected individuals' sera obtained from Ghanaians. HGV RNA in serum was identified by a nested reverse transcription polymerase chain reaction (RT-PCR) using primers derived from the 5′-noncoding region. We also tested for hepatitis C virus by nested RT-PCR and for hepatitis B surface antigen (HBsAg) by passive hemagglutination method. HGV RNA was detected in 17 of 85 (20%) HIV sero-positive and three of 30 (10%) sero-negative Ghanaians, respectively. The prevalence of HGV infection was much greater than hepatitis C (0.9%) and hepatitis B virus (7.8%) infections in the present study. Ninety four percent of HGV infected patients were seronegative for hepatitis B and C virus infections. The nine different Ghanaian isolates in the 5′-untranslated region of the HGV genome had one nucleotide deletion at the same position when compared with other HGV isolates. Phylogenetic analysis showed that all Ghanaian isolates belonged to type 1 (West Africa type) of the HGV genotypes. Moreover, we determined nearly full-length nucleotide sequence of the HGV genome (denoted HGV-GA128) recovered from a Ghanaian infected with HIV. The HGV-GA128 was composed of 9231 nucleotides and had a single open reading frame, encoding 2843 amino acid residues. This isolate differed from previously reported HGV/GBV-C isolates by 10–15% of the nucleotide sequence and 2–5% of the amino acid sequence. Our data indicate a high prevalence of HGV, especially genotype 1, in Ghana.</abstract><qualityIndicators><score>6.748</score><pdfVersion>1.2</pdfVersion><pdfPageSize>542 x 685 pts</pdfPageSize><refBibsNative>true</refBibsNative><keywordCount>4</keywordCount><abstractCharCount>1593</abstractCharCount><pdfWordCount>3844</pdfWordCount><pdfCharCount>22643</pdfCharCount><pdfPageCount>11</pdfPageCount><abstractWordCount>242</abstractWordCount></qualityIndicators><title>Prevalence of hepatitis G virus and characterization of viral genome in Ghana</title><pii><json:string>S1386-6346(98)00095-3</json:string></pii><genre><json:string>research-article</json:string></genre><host><title>Hepatology Research</title><language><json:string>unknown</json:string></language><publicationDate>1999</publicationDate><issn><json:string>1386-6346</json:string></issn><pii><json:string>S1386-6346(00)X0017-4</json:string></pii><volume>13</volume><issue>3</issue><pages><first>221</first><last>231</last></pages><genre><json:string>journal</json:string></genre></host><categories><wos><json:string>science</json:string><json:string>gastroenterology & hepatology</json:string></wos><scienceMetrix><json:string>health sciences</json:string><json:string>clinical medicine</json:string><json:string>gastroenterology & hepatology</json:string></scienceMetrix><inist><json:string>sciences appliquees, technologies et medecines</json:string><json:string>sciences biologiques et medicales</json:string><json:string>sciences medicales</json:string><json:string>pathologie infectieuse</json:string></inist></categories><publicationDate>1999</publicationDate><copyrightDate>1999</copyrightDate><doi><json:string>10.1016/S1386-6346(98)00095-3</json:string></doi><id>73A2BA2DCE7C630A37FEEE65A5CC15D5E68DD821</id><score>1</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/document/73A2BA2DCE7C630A37FEEE65A5CC15D5E68DD821/fulltext/pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/document/73A2BA2DCE7C630A37FEEE65A5CC15D5E68DD821/fulltext/zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/document/73A2BA2DCE7C630A37FEEE65A5CC15D5E68DD821/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a">Prevalence of hepatitis G virus and characterization of viral genome in Ghana</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>ELSEVIER</publisher><availability><p>©1999 Elsevier Science Ireland Ltd</p></availability><date>1999</date></publicationStmt><notesStmt><note>The nucleotide sequence data reported in this paper have been deposited in the DDBJ, EMBL and GenBank nucleotide sequence databases with the accession number AB013500 for HGV-GA128, AB004508 for HGV-GA1, AB004509 for HGV-GA22, AB004510 for HGV-GA53, AB004511 for HGV-GA56, AB004512 for HGV-GA6, AB004513 for HGV-GA62, AB004514 for HGV-GA9, AB004515 for HGV-GA94 and AB004516 for HGV-GA95.</note><note type="content">Fig. 1: Alignment of the nucleotide sequences of 5′-UTR from HGV isolates in this study and from those reported previously. HGV-PNF2161 and R10291 are American isolates and GBV-C isolate is derived from West african patient, reported previously. Others are Ghanaian isolates in this study. Nucleotide numbers are according to HGV-PNF2161 reported by Linnen et al. [1]. Dashes represent nucleotides that are identical to those of HGV prototype (top line); /: deletion.</note><note type="content">Fig. 2: Phylograms generated by neighbor-joining analysis of genetic distances in full and nearly full genome nucleotide sequence of HGV/GBV-C isolates. Unclassified isolate is presented in italic (CG12LC). The percentage of bootstrap replicates supporting these branches is shown in number. Source of all isolates with the accession numbers was described in the text.</note><note type="content">Table 1: Prevalence of HGV, HCV and HBV among Ghanaian individuals</note><note type="content">Table 2: Frequency of HCV and HBV infections among HGV-positive Ghanaians</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a">Prevalence of hepatitis G virus and characterization of viral genome in Ghana</title><author xml:id="author-0000"><persName><forename type="first">Takahide</forename><surname>Saito</surname></persName><affiliation>Department of Pathology, National Institute of Infectious Diseases, Toyama 1-23-1, Shinjuku-ku, Tokyo 162-8640, Japan</affiliation></author><author xml:id="author-0001"><persName><forename type="first">Koh-ichi</forename><surname>Ishikawa</surname></persName><affiliation>AIDS Research Center, National Institute of Infectious Diseases, Toyama 1-23-1, Shinjuku-ku, Tokyo 162-8640, Japan</affiliation></author><author xml:id="author-0002"><persName><forename type="first">Mubarak</forename><surname>Osei-Kwasi</surname></persName><affiliation>The Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Ghana</affiliation></author><author xml:id="author-0003"><persName><forename type="first">Tamiko</forename><surname>Kaneko</surname></persName><affiliation>Department of Pathology, National Institute of Infectious Diseases, Toyama 1-23-1, Shinjuku-ku, Tokyo 162-8640, Japan</affiliation></author><author xml:id="author-0004"><persName><forename type="first">James A.M</forename><surname>Brandful</surname></persName><affiliation>The Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Ghana</affiliation></author><author xml:id="author-0005"><persName><forename type="first">Victor</forename><surname>Nuvor</surname></persName><affiliation>The Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Ghana</affiliation></author><author xml:id="author-0006"><persName><forename type="first">Simeon</forename><surname>Aidoo</surname></persName><affiliation>The Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Ghana</affiliation></author><author xml:id="author-0007"><persName><forename type="first">William</forename><surname>Ampofo</surname></persName><affiliation>The Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Ghana</affiliation></author><author xml:id="author-0008"><persName><forename type="first">Frank A</forename><surname>Apeagyei</surname></persName><affiliation>Army Health Unit, 37 Military Hospital, Accra, Ghana</affiliation></author><author xml:id="author-0009"><persName><forename type="first">Jane E</forename><surname>Ansah</surname></persName><affiliation>Army Health Unit, 37 Military Hospital, Accra, Ghana</affiliation></author><author xml:id="author-0010"><persName><forename type="first">Yaw</forename><surname>Adu-Sarkodie</surname></persName><affiliation>Department of Microbiology, Komfo Anokye Teaching Hospital, Kumasi, Ghana</affiliation></author><author xml:id="author-0011"><persName><forename type="first">Francis K</forename><surname>Nkrumah</surname></persName><affiliation>The Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Ghana</affiliation></author><author xml:id="author-0012"><persName><forename type="first">Kenji</forename><surname>Abe</surname></persName><note type="correspondence"><p>Corresponding author. Tel.: +81 3 52851111 ext. 2624; fax: +81 3 52851189; e-mail: kenjiabe@nih.go.jp</p></note><affiliation>Department of Pathology, National Institute of Infectious Diseases, Toyama 1-23-1, Shinjuku-ku, Tokyo 162-8640, Japan</affiliation></author><idno type="istex">73A2BA2DCE7C630A37FEEE65A5CC15D5E68DD821</idno><idno type="DOI">10.1016/S1386-6346(98)00095-3</idno><idno type="PII">S1386-6346(98)00095-3</idno></analytic><monogr><title level="j">Hepatology Research</title><title level="j" type="abbrev">HEPC</title><idno type="pISSN">1386-6346</idno><idno type="PII">S1386-6346(00)X0017-4</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1999"></date><biblScope unit="volume">13</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="221">221</biblScope><biblScope unit="page" to="231">231</biblScope></imprint></monogr></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1999</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>The prevalence of hepatitis G virus (HGV) infection was investigated in 85 human immunodeficiency virus (HIV)-infected and 30 uninfected individuals' sera obtained from Ghanaians. HGV RNA in serum was identified by a nested reverse transcription polymerase chain reaction (RT-PCR) using primers derived from the 5′-noncoding region. We also tested for hepatitis C virus by nested RT-PCR and for hepatitis B surface antigen (HBsAg) by passive hemagglutination method. HGV RNA was detected in 17 of 85 (20%) HIV sero-positive and three of 30 (10%) sero-negative Ghanaians, respectively. The prevalence of HGV infection was much greater than hepatitis C (0.9%) and hepatitis B virus (7.8%) infections in the present study. Ninety four percent of HGV infected patients were seronegative for hepatitis B and C virus infections. The nine different Ghanaian isolates in the 5′-untranslated region of the HGV genome had one nucleotide deletion at the same position when compared with other HGV isolates. Phylogenetic analysis showed that all Ghanaian isolates belonged to type 1 (West Africa type) of the HGV genotypes. Moreover, we determined nearly full-length nucleotide sequence of the HGV genome (denoted HGV-GA128) recovered from a Ghanaian infected with HIV. The HGV-GA128 was composed of 9231 nucleotides and had a single open reading frame, encoding 2843 amino acid residues. This isolate differed from previously reported HGV/GBV-C isolates by 10–15% of the nucleotide sequence and 2–5% of the amino acid sequence. Our data indicate a high prevalence of HGV, especially genotype 1, in Ghana.</p></abstract><textClass><keywords scheme="keyword"><list><head>Keywords</head><item><term>Full-length sequence of Ghanaian HGV isolate (HGV-GA128)</term></item><item><term>Ghana</term></item><item><term>Hepatitis G virus (HGV) genome</term></item><item><term>HGV genotypes</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="1998-08-27">Modified</change><change when="1999">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/73A2BA2DCE7C630A37FEEE65A5CC15D5E68DD821/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: ce:floats; body; tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"><istex:entity SYSTEM="gr1" NDATA="IMAGE" name="gr1"></istex:entity><istex:entity SYSTEM="gr2" NDATA="IMAGE" name="gr2"></istex:entity></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla"><item-info><jid>HEPC</jid><aid>551</aid><ce:pii>S1386-6346(98)00095-3</ce:pii><ce:doi>10.1016/S1386-6346(98)00095-3</ce:doi><ce:copyright year="1999" type="full-transfer">Elsevier Science Ireland Ltd</ce:copyright></item-info><head><ce:title>Prevalence of hepatitis G virus and characterization of viral genome in Ghana<ce:footnote id="FN1"><ce:label>1</ce:label><ce:note-para>The nucleotide sequence data reported in this paper have been deposited in the DDBJ, EMBL and GenBank nucleotide sequence databases with the accession number AB013500 for HGV-GA128, AB004508 for HGV-GA1, AB004509 for HGV-GA22, AB004510 for HGV-GA53, AB004511 for HGV-GA56, AB004512 for HGV-GA6, AB004513 for HGV-GA62, AB004514 for HGV-GA9, AB004515 for HGV-GA94 and AB004516 for HGV-GA95.</ce:note-para></ce:footnote><ce:cross-ref refid="FN1"><ce:sup>1</ce:sup></ce:cross-ref></ce:title><ce:author-group><ce:author><ce:given-name>Takahide</ce:given-name><ce:surname>Saito</ce:surname><ce:cross-ref refid="AFF1">a</ce:cross-ref></ce:author><ce:author><ce:given-name>Koh-ichi</ce:given-name><ce:surname>Ishikawa</ce:surname><ce:cross-ref refid="AFF2">b</ce:cross-ref></ce:author><ce:author><ce:given-name>Mubarak</ce:given-name><ce:surname>Osei-Kwasi</ce:surname><ce:cross-ref refid="AFF3">c</ce:cross-ref></ce:author><ce:author><ce:given-name>Tamiko</ce:given-name><ce:surname>Kaneko</ce:surname><ce:cross-ref refid="AFF1">a</ce:cross-ref></ce:author><ce:author><ce:given-name>James A.M</ce:given-name><ce:surname>Brandful</ce:surname><ce:cross-ref refid="AFF3">c</ce:cross-ref></ce:author><ce:author><ce:given-name>Victor</ce:given-name><ce:surname>Nuvor</ce:surname><ce:cross-ref refid="AFF3">c</ce:cross-ref></ce:author><ce:author><ce:given-name>Simeon</ce:given-name><ce:surname>Aidoo</ce:surname><ce:cross-ref refid="AFF3">c</ce:cross-ref></ce:author><ce:author><ce:given-name>William</ce:given-name><ce:surname>Ampofo</ce:surname><ce:cross-ref refid="AFF3">c</ce:cross-ref></ce:author><ce:author><ce:given-name>Frank A</ce:given-name><ce:surname>Apeagyei</ce:surname><ce:cross-ref refid="AFF4">d</ce:cross-ref></ce:author><ce:author><ce:given-name>Jane E</ce:given-name><ce:surname>Ansah</ce:surname><ce:cross-ref refid="AFF4">d</ce:cross-ref></ce:author><ce:author><ce:given-name>Yaw</ce:given-name><ce:surname>Adu-Sarkodie</ce:surname><ce:cross-ref refid="AFF5">e</ce:cross-ref></ce:author><ce:author><ce:given-name>Francis K</ce:given-name><ce:surname>Nkrumah</ce:surname><ce:cross-ref refid="AFF3">c</ce:cross-ref></ce:author><ce:author><ce:given-name>Kenji</ce:given-name><ce:surname>Abe</ce:surname><ce:cross-ref refid="AFF1">a</ce:cross-ref><ce:cross-ref refid="CORR1">*</ce:cross-ref></ce:author><ce:affiliation id="AFF1"><ce:label>a</ce:label><ce:textfn>Department of Pathology, National Institute of Infectious Diseases, Toyama 1-23-1, Shinjuku-ku, Tokyo 162-8640, Japan</ce:textfn></ce:affiliation><ce:affiliation id="AFF2"><ce:label>b</ce:label><ce:textfn>AIDS Research Center, National Institute of Infectious Diseases, Toyama 1-23-1, Shinjuku-ku, Tokyo 162-8640, Japan</ce:textfn></ce:affiliation><ce:affiliation id="AFF3"><ce:label>c</ce:label><ce:textfn>The Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Ghana</ce:textfn></ce:affiliation><ce:affiliation id="AFF4"><ce:label>d</ce:label><ce:textfn>Army Health Unit, 37 Military Hospital, Accra, Ghana</ce:textfn></ce:affiliation><ce:affiliation id="AFF5"><ce:label>e</ce:label><ce:textfn>Department of Microbiology, Komfo Anokye Teaching Hospital, Kumasi, Ghana</ce:textfn></ce:affiliation><ce:correspondence id="CORR1"><ce:label>*</ce:label><ce:text>Corresponding author. Tel.: +81 3 52851111 ext. 2624; fax: +81 3 52851189; e-mail: kenjiabe@nih.go.jp</ce:text></ce:correspondence></ce:author-group><ce:date-received day="21" month="7" year="1998"></ce:date-received><ce:date-revised day="27" month="8" year="1998"></ce:date-revised><ce:date-accepted day="18" month="9" year="1998"></ce:date-accepted><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>The prevalence of hepatitis G virus (HGV) infection was investigated in 85 human immunodeficiency virus (HIV)-infected and 30 uninfected individuals' sera obtained from Ghanaians. HGV RNA in serum was identified by a nested reverse transcription polymerase chain reaction (RT-PCR) using primers derived from the 5′-noncoding region. We also tested for hepatitis C virus by nested RT-PCR and for hepatitis B surface antigen (HBsAg) by passive hemagglutination method. HGV RNA was detected in 17 of 85 (20%) HIV sero-positive and three of 30 (10%) sero-negative Ghanaians, respectively. The prevalence of HGV infection was much greater than hepatitis C (0.9%) and hepatitis B virus (7.8%) infections in the present study. Ninety four percent of HGV infected patients were seronegative for hepatitis B and C virus infections. The nine different Ghanaian isolates in the 5′-untranslated region of the HGV genome had one nucleotide deletion at the same position when compared with other HGV isolates. Phylogenetic analysis showed that all Ghanaian isolates belonged to type 1 (West Africa type) of the HGV genotypes. Moreover, we determined nearly full-length nucleotide sequence of the HGV genome (denoted HGV-GA128) recovered from a Ghanaian infected with HIV. The HGV-GA128 was composed of 9231 nucleotides and had a single open reading frame, encoding 2843 amino acid residues. This isolate differed from previously reported HGV/GBV-C isolates by 10–15% of the nucleotide sequence and 2–5% of the amino acid sequence. Our data indicate a high prevalence of HGV, especially genotype 1, in Ghana.</ce:simple-para></ce:abstract-sec></ce:abstract><ce:keywords class="keyword"><ce:section-title>Keywords</ce:section-title><ce:keyword><ce:text>Full-length sequence of Ghanaian HGV isolate (HGV-GA128)</ce:text></ce:keyword><ce:keyword><ce:text>Ghana</ce:text></ce:keyword><ce:keyword><ce:text>Hepatitis G virus (HGV) genome</ce:text></ce:keyword><ce:keyword><ce:text>HGV genotypes</ce:text></ce:keyword></ce:keywords></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><title>Prevalence of hepatitis G virus and characterization of viral genome in Ghana</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Prevalence of hepatitis G virus and characterization of viral genome in Ghana</title></titleInfo><name type="personal"><namePart type="given">Takahide</namePart><namePart type="family">Saito</namePart><affiliation>Department of Pathology, National Institute of Infectious Diseases, Toyama 1-23-1, Shinjuku-ku, Tokyo 162-8640, Japan</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Koh-ichi</namePart><namePart type="family">Ishikawa</namePart><affiliation>AIDS Research Center, National Institute of Infectious Diseases, Toyama 1-23-1, Shinjuku-ku, Tokyo 162-8640, Japan</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Mubarak</namePart><namePart type="family">Osei-Kwasi</namePart><affiliation>The Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Ghana</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Tamiko</namePart><namePart type="family">Kaneko</namePart><affiliation>Department of Pathology, National Institute of Infectious Diseases, Toyama 1-23-1, Shinjuku-ku, Tokyo 162-8640, Japan</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">James A.M</namePart><namePart type="family">Brandful</namePart><affiliation>The Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Ghana</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Victor</namePart><namePart type="family">Nuvor</namePart><affiliation>The Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Ghana</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Simeon</namePart><namePart type="family">Aidoo</namePart><affiliation>The Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Ghana</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">William</namePart><namePart type="family">Ampofo</namePart><affiliation>The Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Ghana</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Frank A</namePart><namePart type="family">Apeagyei</namePart><affiliation>Army Health Unit, 37 Military Hospital, Accra, Ghana</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Jane E</namePart><namePart type="family">Ansah</namePart><affiliation>Army Health Unit, 37 Military Hospital, Accra, Ghana</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Yaw</namePart><namePart type="family">Adu-Sarkodie</namePart><affiliation>Department of Microbiology, Komfo Anokye Teaching Hospital, Kumasi, Ghana</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Francis K</namePart><namePart type="family">Nkrumah</namePart><affiliation>The Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Ghana</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Kenji</namePart><namePart type="family">Abe</namePart><affiliation>Department of Pathology, National Institute of Infectious Diseases, Toyama 1-23-1, Shinjuku-ku, Tokyo 162-8640, Japan</affiliation><description>Corresponding author. Tel.: +81 3 52851111 ext. 2624; fax: +81 3 52851189; e-mail: kenjiabe@nih.go.jp</description><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article"></genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1999</dateIssued><dateModified encoding="w3cdtf">1998-08-27</dateModified><copyrightDate encoding="w3cdtf">1999</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType></physicalDescription><abstract lang="en">The prevalence of hepatitis G virus (HGV) infection was investigated in 85 human immunodeficiency virus (HIV)-infected and 30 uninfected individuals' sera obtained from Ghanaians. HGV RNA in serum was identified by a nested reverse transcription polymerase chain reaction (RT-PCR) using primers derived from the 5′-noncoding region. We also tested for hepatitis C virus by nested RT-PCR and for hepatitis B surface antigen (HBsAg) by passive hemagglutination method. HGV RNA was detected in 17 of 85 (20%) HIV sero-positive and three of 30 (10%) sero-negative Ghanaians, respectively. The prevalence of HGV infection was much greater than hepatitis C (0.9%) and hepatitis B virus (7.8%) infections in the present study. Ninety four percent of HGV infected patients were seronegative for hepatitis B and C virus infections. The nine different Ghanaian isolates in the 5′-untranslated region of the HGV genome had one nucleotide deletion at the same position when compared with other HGV isolates. Phylogenetic analysis showed that all Ghanaian isolates belonged to type 1 (West Africa type) of the HGV genotypes. Moreover, we determined nearly full-length nucleotide sequence of the HGV genome (denoted HGV-GA128) recovered from a Ghanaian infected with HIV. The HGV-GA128 was composed of 9231 nucleotides and had a single open reading frame, encoding 2843 amino acid residues. This isolate differed from previously reported HGV/GBV-C isolates by 10–15% of the nucleotide sequence and 2–5% of the amino acid sequence. Our data indicate a high prevalence of HGV, especially genotype 1, in Ghana.</abstract><note type="footnote">The nucleotide sequence data reported in this paper have been deposited in the DDBJ, EMBL and GenBank nucleotide sequence databases with the accession number AB013500 for HGV-GA128, AB004508 for HGV-GA1, AB004509 for HGV-GA22, AB004510 for HGV-GA53, AB004511 for HGV-GA56, AB004512 for HGV-GA6, AB004513 for HGV-GA62, AB004514 for HGV-GA9, AB004515 for HGV-GA94 and AB004516 for HGV-GA95.</note><note type="content">Fig. 1: Alignment of the nucleotide sequences of 5′-UTR from HGV isolates in this study and from those reported previously. HGV-PNF2161 and R10291 are American isolates and GBV-C isolate is derived from West african patient, reported previously. Others are Ghanaian isolates in this study. Nucleotide numbers are according to HGV-PNF2161 reported by Linnen et al. [1]. Dashes represent nucleotides that are identical to those of HGV prototype (top line); /: deletion.</note><note type="content">Fig. 2: Phylograms generated by neighbor-joining analysis of genetic distances in full and nearly full genome nucleotide sequence of HGV/GBV-C isolates. Unclassified isolate is presented in italic (CG12LC). The percentage of bootstrap replicates supporting these branches is shown in number. Source of all isolates with the accession numbers was described in the text.</note><note type="content">Table 1: Prevalence of HGV, HCV and HBV among Ghanaian individuals</note><note type="content">Table 2: Frequency of HCV and HBV infections among HGV-positive Ghanaians</note><subject><genre>Keywords</genre><topic>Full-length sequence of Ghanaian HGV isolate (HGV-GA128)</topic><topic>Ghana</topic><topic>Hepatitis G virus (HGV) genome</topic><topic>HGV genotypes</topic></subject><relatedItem type="host"><titleInfo><title>Hepatology Research</title></titleInfo><titleInfo type="abbreviated"><title>HEPC</title></titleInfo><genre type="journal">journal</genre><originInfo><dateIssued encoding="w3cdtf">199902</dateIssued></originInfo><identifier type="ISSN">1386-6346</identifier><identifier type="PII">S1386-6346(00)X0017-4</identifier><part><date>199902</date><detail type="volume"><number>13</number><caption>vol.</caption></detail><detail type="issue"><number>3</number><caption>no.</caption></detail><extent unit="issue pages"><start>183</start><end>276</end></extent><extent unit="pages"><start>221</start><end>231</end></extent></part></relatedItem><identifier type="istex">73A2BA2DCE7C630A37FEEE65A5CC15D5E68DD821</identifier><identifier type="DOI">10.1016/S1386-6346(98)00095-3</identifier><identifier type="PII">S1386-6346(98)00095-3</identifier><accessCondition type="use and reproduction" contentType="copyright">©1999 Elsevier Science Ireland Ltd</accessCondition><recordInfo><recordContentSource>ELSEVIER</recordContentSource><recordOrigin>Elsevier Science Ireland Ltd, ©1999</recordOrigin></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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