Biological characterization of human immunodeficiency virus type 1 and type 2 mutants in human peripheral blood mononuclear cells
Identifieur interne : 000083 ( Istex/Corpus ); précédent : 000082; suivant : 000084Biological characterization of human immunodeficiency virus type 1 and type 2 mutants in human peripheral blood mononuclear cells
Auteurs : H. Akari ; J. Sakuragi ; Y. Takebe ; K. Tomonaga ; M. Kawamura ; M. Fukasawa ; T. Miura ; T. Shinjo ; M. HayamiSource :
- Archives of Virology [ 0304-8608 ] ; 1992-03-01.
English descriptors
- KwdEn :
- Acad, Akari, Cell lines, Clone, Equivalent amount, Gene product, Growth kinetics, Haseltine, Human immunodeficiency virus, Human immunodeficiency virus type, Immunodeficiency, Insertion, Kinetics, Kyoto university, Molt3 cells, Mutant, Mutation, Mutational analysis, Natl, Nefgene, Negative control, Pbmc, Plasmid, Proc, Proc natl acad, Replication, Syncytium, Syncytium formation, Transfected, Virol, Virus, Virus replication, Wild type, Wild type clone.
- Teeft :
- Acad, Akari, Cell lines, Clone, Equivalent amount, Gene product, Growth kinetics, Haseltine, Human immunodeficiency virus, Human immunodeficiency virus type, Immunodeficiency, Insertion, Kinetics, Kyoto university, Molt3 cells, Mutant, Mutation, Mutational analysis, Natl, Nefgene, Negative control, Pbmc, Plasmid, Proc, Proc natl acad, Replication, Syncytium, Syncytium formation, Transfected, Virol, Virus, Virus replication, Wild type, Wild type clone.
Abstract
Summary: Mutants of human immunodeficiency virus type 1 (HIV-1) and type 2 (HIV-2), which have been shown to be infectious in established cell lines, were tested for ability to replicate and induce syncytium formation in human peripheral blood mononuclear cells (PBMC). Thevpu mutant of HIV-1 showed depressed kinetics of replication in an established T cell line, as reported previously, but in PBMC, its replication was similar to that of the wild type virus. Thevpx gene of HIV-2 was required for efficient virus propagation in PBMC, but not in an established T cell line, as previously reported. However, the growth rates of thevpx mutant in PBMC preparations from two individuals were different. The results of experiments on infection of PBMC with thevif andvpr mutants of HIV-1 and HIV-2 were essentially consistent with previous results of infection of established T cell lines. No negative effect of thenef gene products of HIV-1 and HIV-2 was observed. The abilities of the wild type virus and the mutants of HIV-1 to induce syncytium formation in both PBMC and established cell lines were similar. In contrast, neither the wild type nor any of the mutants of HIV-2 induced syncytium formation in PBMC. These results suggest that the functions of some genes can be detected only in mixed populations or primary cells such as PBMC. Studies on the roles of these genes in PBMC may provide a better understanding of their functions in vivo.
Url:
DOI: 10.1007/BF01317146
Links to Exploration step
ISTEX:4D4EEBB15A120CC49AA38C1FAE57CE835767985ALe document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Biological characterization of human immunodeficiency virus type 1 and type 2 mutants in human peripheral blood mononuclear cells</title><author><name sortKey="Akari, H" sort="Akari, H" uniqKey="Akari H" first="H." last="Akari">H. Akari</name><affiliation><mods:affiliation>Research Center for Immunodeficiency Virus, Institute for Virus Research, Kyoto University, Kyoto</mods:affiliation></affiliation><affiliation><mods:affiliation>Department of Veterinary Medicine, Faculty of Agriculture, Miyazaki University, Miyazaki, Japan</mods:affiliation></affiliation><affiliation><mods:affiliation>Department of Biodefence and Medical Virology, Kumamoto University Medical School, 860, Kumamoto, Japan</mods:affiliation></affiliation></author><author><name sortKey="Sakuragi, J" sort="Sakuragi, J" uniqKey="Sakuragi J" first="J." last="Sakuragi">J. Sakuragi</name><affiliation><mods:affiliation>Research Center for Immunodeficiency Virus, Institute for Virus Research, Kyoto University, Kyoto</mods:affiliation></affiliation></author><author><name sortKey="Takebe, Y" sort="Takebe, Y" uniqKey="Takebe Y" first="Y." last="Takebe">Y. Takebe</name><affiliation><mods:affiliation>AIDS Research Center, National Institute of Health, Tokyo</mods:affiliation></affiliation></author><author><name sortKey="Tomonaga, K" sort="Tomonaga, K" uniqKey="Tomonaga K" first="K." last="Tomonaga">K. Tomonaga</name><affiliation><mods:affiliation>Research Center for Immunodeficiency Virus, Institute for Virus Research, Kyoto University, Kyoto</mods:affiliation></affiliation></author><author><name sortKey="Kawamura, M" sort="Kawamura, M" uniqKey="Kawamura M" first="M." last="Kawamura">M. Kawamura</name><affiliation><mods:affiliation>Research Center for Immunodeficiency Virus, Institute for Virus Research, Kyoto University, Kyoto</mods:affiliation></affiliation></author><author><name sortKey="Fukasawa, M" sort="Fukasawa, M" uniqKey="Fukasawa M" first="M." last="Fukasawa">M. Fukasawa</name><affiliation><mods:affiliation>Research Center for Immunodeficiency Virus, Institute for Virus Research, Kyoto University, Kyoto</mods:affiliation></affiliation></author><author><name sortKey="Miura, T" sort="Miura, T" uniqKey="Miura T" first="T." last="Miura">T. Miura</name><affiliation><mods:affiliation>Research Center for Immunodeficiency Virus, Institute for Virus Research, Kyoto University, Kyoto</mods:affiliation></affiliation></author><author><name sortKey="Shinjo, T" sort="Shinjo, T" uniqKey="Shinjo T" first="T." last="Shinjo">T. Shinjo</name><affiliation><mods:affiliation>Department of Veterinary Medicine, Faculty of Agriculture, Miyazaki University, Miyazaki, Japan</mods:affiliation></affiliation></author><author><name sortKey="Hayami, M" sort="Hayami, M" uniqKey="Hayami M" first="M." last="Hayami">M. Hayami</name><affiliation><mods:affiliation>Research Center for Immunodeficiency Virus, Institute for Virus Research, Kyoto University, Kyoto</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:4D4EEBB15A120CC49AA38C1FAE57CE835767985A</idno><date when="1992" year="1992">1992</date><idno type="doi">10.1007/BF01317146</idno><idno type="url">https://api.istex.fr/document/4D4EEBB15A120CC49AA38C1FAE57CE835767985A/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000083</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000083</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Biological characterization of human immunodeficiency virus type 1 and type 2 mutants in human peripheral blood mononuclear cells</title><author><name sortKey="Akari, H" sort="Akari, H" uniqKey="Akari H" first="H." last="Akari">H. Akari</name><affiliation><mods:affiliation>Research Center for Immunodeficiency Virus, Institute for Virus Research, Kyoto University, Kyoto</mods:affiliation></affiliation><affiliation><mods:affiliation>Department of Veterinary Medicine, Faculty of Agriculture, Miyazaki University, Miyazaki, Japan</mods:affiliation></affiliation><affiliation><mods:affiliation>Department of Biodefence and Medical Virology, Kumamoto University Medical School, 860, Kumamoto, Japan</mods:affiliation></affiliation></author><author><name sortKey="Sakuragi, J" sort="Sakuragi, J" uniqKey="Sakuragi J" first="J." last="Sakuragi">J. Sakuragi</name><affiliation><mods:affiliation>Research Center for Immunodeficiency Virus, Institute for Virus Research, Kyoto University, Kyoto</mods:affiliation></affiliation></author><author><name sortKey="Takebe, Y" sort="Takebe, Y" uniqKey="Takebe Y" first="Y." last="Takebe">Y. Takebe</name><affiliation><mods:affiliation>AIDS Research Center, National Institute of Health, Tokyo</mods:affiliation></affiliation></author><author><name sortKey="Tomonaga, K" sort="Tomonaga, K" uniqKey="Tomonaga K" first="K." last="Tomonaga">K. Tomonaga</name><affiliation><mods:affiliation>Research Center for Immunodeficiency Virus, Institute for Virus Research, Kyoto University, Kyoto</mods:affiliation></affiliation></author><author><name sortKey="Kawamura, M" sort="Kawamura, M" uniqKey="Kawamura M" first="M." last="Kawamura">M. Kawamura</name><affiliation><mods:affiliation>Research Center for Immunodeficiency Virus, Institute for Virus Research, Kyoto University, Kyoto</mods:affiliation></affiliation></author><author><name sortKey="Fukasawa, M" sort="Fukasawa, M" uniqKey="Fukasawa M" first="M." last="Fukasawa">M. Fukasawa</name><affiliation><mods:affiliation>Research Center for Immunodeficiency Virus, Institute for Virus Research, Kyoto University, Kyoto</mods:affiliation></affiliation></author><author><name sortKey="Miura, T" sort="Miura, T" uniqKey="Miura T" first="T." last="Miura">T. Miura</name><affiliation><mods:affiliation>Research Center for Immunodeficiency Virus, Institute for Virus Research, Kyoto University, Kyoto</mods:affiliation></affiliation></author><author><name sortKey="Shinjo, T" sort="Shinjo, T" uniqKey="Shinjo T" first="T." last="Shinjo">T. Shinjo</name><affiliation><mods:affiliation>Department of Veterinary Medicine, Faculty of Agriculture, Miyazaki University, Miyazaki, Japan</mods:affiliation></affiliation></author><author><name sortKey="Hayami, M" sort="Hayami, M" uniqKey="Hayami M" first="M." last="Hayami">M. Hayami</name><affiliation><mods:affiliation>Research Center for Immunodeficiency Virus, Institute for Virus Research, Kyoto University, Kyoto</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Archives of Virology</title><title level="j" type="abbrev">Archives of Virology</title><idno type="ISSN">0304-8608</idno><idno type="eISSN">1432-8798</idno><imprint><publisher>Springer-Verlag</publisher><pubPlace>Vienna</pubPlace><date type="published" when="1992-03-01">1992-03-01</date><biblScope unit="volume">123</biblScope><biblScope unit="issue">1-2</biblScope><biblScope unit="page" from="157">157</biblScope><biblScope unit="page" to="167">167</biblScope></imprint><idno type="ISSN">0304-8608</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0304-8608</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Acad</term><term>Akari</term><term>Cell lines</term><term>Clone</term><term>Equivalent amount</term><term>Gene product</term><term>Growth kinetics</term><term>Haseltine</term><term>Human immunodeficiency virus</term><term>Human immunodeficiency virus type</term><term>Immunodeficiency</term><term>Insertion</term><term>Kinetics</term><term>Kyoto university</term><term>Molt3 cells</term><term>Mutant</term><term>Mutation</term><term>Mutational analysis</term><term>Natl</term><term>Nefgene</term><term>Negative control</term><term>Pbmc</term><term>Plasmid</term><term>Proc</term><term>Proc natl acad</term><term>Replication</term><term>Syncytium</term><term>Syncytium formation</term><term>Transfected</term><term>Virol</term><term>Virus</term><term>Virus replication</term><term>Wild type</term><term>Wild type clone</term></keywords><keywords scheme="Teeft" xml:lang="en"><term>Acad</term><term>Akari</term><term>Cell lines</term><term>Clone</term><term>Equivalent amount</term><term>Gene product</term><term>Growth kinetics</term><term>Haseltine</term><term>Human immunodeficiency virus</term><term>Human immunodeficiency virus type</term><term>Immunodeficiency</term><term>Insertion</term><term>Kinetics</term><term>Kyoto university</term><term>Molt3 cells</term><term>Mutant</term><term>Mutation</term><term>Mutational analysis</term><term>Natl</term><term>Nefgene</term><term>Negative control</term><term>Pbmc</term><term>Plasmid</term><term>Proc</term><term>Proc natl acad</term><term>Replication</term><term>Syncytium</term><term>Syncytium formation</term><term>Transfected</term><term>Virol</term><term>Virus</term><term>Virus replication</term><term>Wild type</term><term>Wild type clone</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Summary: Mutants of human immunodeficiency virus type 1 (HIV-1) and type 2 (HIV-2), which have been shown to be infectious in established cell lines, were tested for ability to replicate and induce syncytium formation in human peripheral blood mononuclear cells (PBMC). Thevpu mutant of HIV-1 showed depressed kinetics of replication in an established T cell line, as reported previously, but in PBMC, its replication was similar to that of the wild type virus. Thevpx gene of HIV-2 was required for efficient virus propagation in PBMC, but not in an established T cell line, as previously reported. However, the growth rates of thevpx mutant in PBMC preparations from two individuals were different. The results of experiments on infection of PBMC with thevif andvpr mutants of HIV-1 and HIV-2 were essentially consistent with previous results of infection of established T cell lines. No negative effect of thenef gene products of HIV-1 and HIV-2 was observed. The abilities of the wild type virus and the mutants of HIV-1 to induce syncytium formation in both PBMC and established cell lines were similar. In contrast, neither the wild type nor any of the mutants of HIV-2 induced syncytium formation in PBMC. These results suggest that the functions of some genes can be detected only in mixed populations or primary cells such as PBMC. Studies on the roles of these genes in PBMC may provide a better understanding of their functions in vivo.</div></front></TEI><istex><corpusName>springer</corpusName><keywords><teeft><json:string>mutant</json:string><json:string>pbmc</json:string><json:string>immunodeficiency</json:string><json:string>cell lines</json:string><json:string>kinetics</json:string><json:string>clone</json:string><json:string>replication</json:string><json:string>virus replication</json:string><json:string>virol</json:string><json:string>syncytium</json:string><json:string>transfected</json:string><json:string>mutation</json:string><json:string>growth kinetics</json:string><json:string>syncytium formation</json:string><json:string>human immunodeficiency virus type</json:string><json:string>proc</json:string><json:string>plasmid</json:string><json:string>acad</json:string><json:string>akari</json:string><json:string>natl</json:string><json:string>proc natl acad</json:string><json:string>haseltine</json:string><json:string>nefgene</json:string><json:string>human immunodeficiency virus</json:string><json:string>wild type clone</json:string><json:string>gene product</json:string><json:string>negative control</json:string><json:string>wild type</json:string><json:string>insertion</json:string><json:string>equivalent amount</json:string><json:string>molt3 cells</json:string><json:string>kyoto university</json:string><json:string>mutational analysis</json:string><json:string>virus</json:string></teeft></keywords><author><json:item><name>H. Akari</name><affiliations><json:string>Research Center for Immunodeficiency Virus, Institute for Virus Research, Kyoto University, Kyoto</json:string><json:string>Department of Veterinary Medicine, Faculty of Agriculture, Miyazaki University, Miyazaki, Japan</json:string><json:string>Department of Biodefence and Medical Virology, Kumamoto University Medical School, 860, Kumamoto, Japan</json:string></affiliations></json:item><json:item><name>J. Sakuragi</name><affiliations><json:string>Research Center for Immunodeficiency Virus, Institute for Virus Research, Kyoto University, Kyoto</json:string></affiliations></json:item><json:item><name>Y. Takebe</name><affiliations><json:string>AIDS Research Center, National Institute of Health, Tokyo</json:string></affiliations></json:item><json:item><name>K. Tomonaga</name><affiliations><json:string>Research Center for Immunodeficiency Virus, Institute for Virus Research, Kyoto University, Kyoto</json:string></affiliations></json:item><json:item><name>M. Kawamura</name><affiliations><json:string>Research Center for Immunodeficiency Virus, Institute for Virus Research, Kyoto University, Kyoto</json:string></affiliations></json:item><json:item><name>M. Fukasawa</name><affiliations><json:string>Research Center for Immunodeficiency Virus, Institute for Virus Research, Kyoto University, Kyoto</json:string></affiliations></json:item><json:item><name>T. Miura</name><affiliations><json:string>Research Center for Immunodeficiency Virus, Institute for Virus Research, Kyoto University, Kyoto</json:string></affiliations></json:item><json:item><name>T. Shinjo</name><affiliations><json:string>Department of Veterinary Medicine, Faculty of Agriculture, Miyazaki University, Miyazaki, Japan</json:string></affiliations></json:item><json:item><name>M. Hayami</name><affiliations><json:string>Research Center for Immunodeficiency Virus, Institute for Virus Research, Kyoto University, Kyoto</json:string></affiliations></json:item></author><articleId><json:string>BF01317146</json:string><json:string>Art14</json:string></articleId><language><json:string>eng</json:string></language><originalGenre><json:string>OriginalPaper</json:string></originalGenre><abstract>Summary: Mutants of human immunodeficiency virus type 1 (HIV-1) and type 2 (HIV-2), which have been shown to be infectious in established cell lines, were tested for ability to replicate and induce syncytium formation in human peripheral blood mononuclear cells (PBMC). Thevpu mutant of HIV-1 showed depressed kinetics of replication in an established T cell line, as reported previously, but in PBMC, its replication was similar to that of the wild type virus. Thevpx gene of HIV-2 was required for efficient virus propagation in PBMC, but not in an established T cell line, as previously reported. However, the growth rates of thevpx mutant in PBMC preparations from two individuals were different. The results of experiments on infection of PBMC with thevif andvpr mutants of HIV-1 and HIV-2 were essentially consistent with previous results of infection of established T cell lines. No negative effect of thenef gene products of HIV-1 and HIV-2 was observed. The abilities of the wild type virus and the mutants of HIV-1 to induce syncytium formation in both PBMC and established cell lines were similar. In contrast, neither the wild type nor any of the mutants of HIV-2 induced syncytium formation in PBMC. These results suggest that the functions of some genes can be detected only in mixed populations or primary cells such as PBMC. Studies on the roles of these genes in PBMC may provide a better understanding of their functions in vivo.</abstract><qualityIndicators><score>6.803</score><pdfWordCount>3959</pdfWordCount><pdfCharCount>21374</pdfCharCount><pdfVersion>1.3</pdfVersion><pdfPageCount>11</pdfPageCount><pdfPageSize>562 x 774 pts</pdfPageSize><refBibsNative>false</refBibsNative><abstractWordCount>237</abstractWordCount><abstractCharCount>1447</abstractCharCount><keywordCount>0</keywordCount></qualityIndicators><title>Biological characterization of human immunodeficiency virus type 1 and type 2 mutants in human peripheral blood mononuclear cells</title><genre><json:string>research-article</json:string></genre><host><title>Archives of Virology</title><language><json:string>unknown</json:string></language><publicationDate>1992</publicationDate><copyrightDate>1992</copyrightDate><issn><json:string>0304-8608</json:string></issn><eissn><json:string>1432-8798</json:string></eissn><journalId><json:string>705</json:string></journalId><volume>123</volume><issue>1-2</issue><pages><first>157</first><last>167</last></pages><genre><json:string>journal</json:string></genre><subject><json:item><value>Medical Microbiology</value></json:item><json:item><value>Virology</value></json:item><json:item><value>Infectious Diseases</value></json:item></subject></host><categories><wos><json:string>science</json:string><json:string>virology</json:string></wos><scienceMetrix><json:string>health sciences</json:string><json:string>biomedical research</json:string><json:string>virology</json:string></scienceMetrix><inist><json:string>sciences appliquees, technologies et medecines</json:string><json:string>sciences biologiques et medicales</json:string><json:string>sciences medicales</json:string></inist></categories><publicationDate>1992</publicationDate><copyrightDate>1992</copyrightDate><doi><json:string>10.1007/BF01317146</json:string></doi><id>4D4EEBB15A120CC49AA38C1FAE57CE835767985A</id><score>1</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/document/4D4EEBB15A120CC49AA38C1FAE57CE835767985A/fulltext/pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/document/4D4EEBB15A120CC49AA38C1FAE57CE835767985A/fulltext/zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/document/4D4EEBB15A120CC49AA38C1FAE57CE835767985A/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a" type="main" xml:lang="en">Biological characterization of human immunodeficiency virus type 1 and type 2 mutants in human peripheral blood mononuclear cells</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>Springer-Verlag</publisher><pubPlace>Vienna</pubPlace><availability><p>Springer-Verlag, 1992</p></availability><date>1991-05-31</date></publicationStmt><notesStmt><note>Original Papers</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a" type="main" xml:lang="en">Biological characterization of human immunodeficiency virus type 1 and type 2 mutants in human peripheral blood mononuclear cells</title><author xml:id="author-0000"><persName><forename type="first">H.</forename><surname>Akari</surname></persName><affiliation>Research Center for Immunodeficiency Virus, Institute for Virus Research, Kyoto University, Kyoto</affiliation><affiliation>Department of Veterinary Medicine, Faculty of Agriculture, Miyazaki University, Miyazaki, Japan</affiliation><affiliation>Department of Biodefence and Medical Virology, Kumamoto University Medical School, 860, Kumamoto, Japan</affiliation></author><author xml:id="author-0001"><persName><forename type="first">J.</forename><surname>Sakuragi</surname></persName><affiliation>Research Center for Immunodeficiency Virus, Institute for Virus Research, Kyoto University, Kyoto</affiliation></author><author xml:id="author-0002"><persName><forename type="first">Y.</forename><surname>Takebe</surname></persName><affiliation>AIDS Research Center, National Institute of Health, Tokyo</affiliation></author><author xml:id="author-0003"><persName><forename type="first">K.</forename><surname>Tomonaga</surname></persName><affiliation>Research Center for Immunodeficiency Virus, Institute for Virus Research, Kyoto University, Kyoto</affiliation></author><author xml:id="author-0004"><persName><forename type="first">M.</forename><surname>Kawamura</surname></persName><affiliation>Research Center for Immunodeficiency Virus, Institute for Virus Research, Kyoto University, Kyoto</affiliation></author><author xml:id="author-0005"><persName><forename type="first">M.</forename><surname>Fukasawa</surname></persName><affiliation>Research Center for Immunodeficiency Virus, Institute for Virus Research, Kyoto University, Kyoto</affiliation></author><author xml:id="author-0006"><persName><forename type="first">T.</forename><surname>Miura</surname></persName><affiliation>Research Center for Immunodeficiency Virus, Institute for Virus Research, Kyoto University, Kyoto</affiliation></author><author xml:id="author-0007"><persName><forename type="first">T.</forename><surname>Shinjo</surname></persName><affiliation>Department of Veterinary Medicine, Faculty of Agriculture, Miyazaki University, Miyazaki, Japan</affiliation></author><author xml:id="author-0008"><persName><forename type="first">M.</forename><surname>Hayami</surname></persName><affiliation>Research Center for Immunodeficiency Virus, Institute for Virus Research, Kyoto University, Kyoto</affiliation></author><idno type="istex">4D4EEBB15A120CC49AA38C1FAE57CE835767985A</idno><idno type="DOI">10.1007/BF01317146</idno><idno type="ArticleID">BF01317146</idno><idno type="ArticleID">Art14</idno></analytic><monogr><title level="j">Archives of Virology</title><title level="j" type="abbrev">Archives of Virology</title><idno type="pISSN">0304-8608</idno><idno type="eISSN">1432-8798</idno><idno type="journal-ID">true</idno><idno type="issue-article-count">22</idno><idno type="volume-issue-count">4</idno><imprint><publisher>Springer-Verlag</publisher><pubPlace>Vienna</pubPlace><date type="published" when="1992-03-01"></date><biblScope unit="volume">123</biblScope><biblScope unit="issue">1-2</biblScope><biblScope unit="page" from="157">157</biblScope><biblScope unit="page" to="167">167</biblScope></imprint></monogr></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1991-05-31</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Summary: Mutants of human immunodeficiency virus type 1 (HIV-1) and type 2 (HIV-2), which have been shown to be infectious in established cell lines, were tested for ability to replicate and induce syncytium formation in human peripheral blood mononuclear cells (PBMC). Thevpu mutant of HIV-1 showed depressed kinetics of replication in an established T cell line, as reported previously, but in PBMC, its replication was similar to that of the wild type virus. Thevpx gene of HIV-2 was required for efficient virus propagation in PBMC, but not in an established T cell line, as previously reported. However, the growth rates of thevpx mutant in PBMC preparations from two individuals were different. The results of experiments on infection of PBMC with thevif andvpr mutants of HIV-1 and HIV-2 were essentially consistent with previous results of infection of established T cell lines. No negative effect of thenef gene products of HIV-1 and HIV-2 was observed. The abilities of the wild type virus and the mutants of HIV-1 to induce syncytium formation in both PBMC and established cell lines were similar. In contrast, neither the wild type nor any of the mutants of HIV-2 induced syncytium formation in PBMC. These results suggest that the functions of some genes can be detected only in mixed populations or primary cells such as PBMC. Studies on the roles of these genes in PBMC may provide a better understanding of their functions in vivo.</p></abstract><textClass><keywords scheme="Journal Subject"><list><head>Biomedicine</head><item><term>Medical Microbiology</term></item><item><term>Virology</term></item><item><term>Infectious Diseases</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="1991-05-31">Created</change><change when="1992-03-01">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/4D4EEBB15A120CC49AA38C1FAE57CE835767985A/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Springer, Publisher found" wicri:toSee="no header"><istex:xmlDeclaration>version="1.0" encoding="UTF-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//Springer-Verlag//DTD A++ V2.4//EN" URI="http://devel.springer.de/A++/V2.4/DTD/A++V2.4.dtd" name="istex:docType"></istex:docType><istex:document><Publisher><PublisherInfo><PublisherName>Springer-Verlag</PublisherName><PublisherLocation>Vienna</PublisherLocation></PublisherInfo><Journal><JournalInfo JournalProductType="ArchiveJournal" NumberingStyle="Unnumbered"><JournalID>705</JournalID><JournalPrintISSN>0304-8608</JournalPrintISSN><JournalElectronicISSN>1432-8798</JournalElectronicISSN><JournalTitle>Archives of Virology</JournalTitle><JournalAbbreviatedTitle>Archives of Virology</JournalAbbreviatedTitle><JournalSubjectGroup><JournalSubject Type="Primary">Biomedicine</JournalSubject><JournalSubject Type="Secondary">Medical Microbiology</JournalSubject><JournalSubject Type="Secondary">Virology</JournalSubject><JournalSubject Type="Secondary">Infectious Diseases</JournalSubject></JournalSubjectGroup></JournalInfo><Volume><VolumeInfo VolumeType="Regular" TocLevels="0"><VolumeIDStart>123</VolumeIDStart><VolumeIDEnd>123</VolumeIDEnd><VolumeIssueCount>4</VolumeIssueCount></VolumeInfo><Issue IssueType="Combined"><IssueInfo TocLevels="0"><IssueIDStart>1</IssueIDStart><IssueIDEnd>2</IssueIDEnd><IssueArticleCount>22</IssueArticleCount><IssueHistory><CoverDate><DateString>1992</DateString><Year>1992</Year><Month>3</Month></CoverDate></IssueHistory><IssueCopyright><CopyrightHolderName>Springer-Verlag</CopyrightHolderName><CopyrightYear>1992</CopyrightYear></IssueCopyright></IssueInfo><Article ID="Art14"><ArticleInfo Language="En" ArticleType="OriginalPaper" NumberingStyle="Unnumbered" TocLevels="0" ContainsESM="No"><ArticleID>BF01317146</ArticleID><ArticleDOI>10.1007/BF01317146</ArticleDOI><ArticleSequenceNumber>14</ArticleSequenceNumber><ArticleTitle Language="En">Biological characterization of human immunodeficiency virus type 1 and type 2 mutants in human peripheral blood mononuclear cells</ArticleTitle><ArticleCategory>Original Papers</ArticleCategory><ArticleFirstPage>157</ArticleFirstPage><ArticleLastPage>167</ArticleLastPage><ArticleHistory><RegistrationDate><Year>2005</Year><Month>3</Month><Day>2</Day></RegistrationDate><Received><Year>1991</Year><Month>5</Month><Day>31</Day></Received><Accepted><Year>1991</Year><Month>7</Month><Day>19</Day></Accepted></ArticleHistory><ArticleCopyright><CopyrightHolderName>Springer-Verlag</CopyrightHolderName><CopyrightYear>1992</CopyrightYear></ArticleCopyright><ArticleGrants Type="Regular"><MetadataGrant Grant="OpenAccess"></MetadataGrant><AbstractGrant Grant="OpenAccess"></AbstractGrant><BodyPDFGrant Grant="Restricted"></BodyPDFGrant><BodyHTMLGrant Grant="Restricted"></BodyHTMLGrant><BibliographyGrant Grant="Restricted"></BibliographyGrant><ESMGrant Grant="Restricted"></ESMGrant></ArticleGrants><ArticleContext><JournalID>705</JournalID><VolumeIDStart>123</VolumeIDStart><VolumeIDEnd>123</VolumeIDEnd><IssueIDStart>1</IssueIDStart><IssueIDEnd>2</IssueIDEnd></ArticleContext></ArticleInfo><ArticleHeader><AuthorGroup><Author AffiliationIDS="Aff1 Aff2 Aff3"><AuthorName DisplayOrder="Western"><GivenName>H.</GivenName><FamilyName>Akari</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>J.</GivenName><FamilyName>Sakuragi</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff4"><AuthorName DisplayOrder="Western"><GivenName>Y.</GivenName><FamilyName>Takebe</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>K.</GivenName><FamilyName>Tomonaga</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>M.</GivenName><FamilyName>Kawamura</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>M.</GivenName><FamilyName>Fukasawa</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>T.</GivenName><FamilyName>Miura</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff2"><AuthorName DisplayOrder="Western"><GivenName>T.</GivenName><FamilyName>Shinjo</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>M.</GivenName><FamilyName>Hayami</FamilyName></AuthorName></Author><Affiliation ID="Aff1"><OrgDivision>Research Center for Immunodeficiency Virus, Institute for Virus Research</OrgDivision><OrgName>Kyoto University</OrgName><OrgAddress><City>Kyoto</City></OrgAddress></Affiliation><Affiliation ID="Aff4"><OrgDivision>AIDS Research Center</OrgDivision><OrgName>National Institute of Health</OrgName><OrgAddress><City>Tokyo</City></OrgAddress></Affiliation><Affiliation ID="Aff2"><OrgDivision>Department of Veterinary Medicine, Faculty of Agriculture</OrgDivision><OrgName>Miyazaki University</OrgName><OrgAddress><City>Miyazaki</City><Country>Japan</Country></OrgAddress></Affiliation><Affiliation ID="Aff3"><OrgDivision>Department of Biodefence and Medical Virology</OrgDivision><OrgName>Kumamoto University Medical School</OrgName><OrgAddress><Postcode>860</Postcode><City>Kumamoto</City><Country>Japan</Country></OrgAddress></Affiliation></AuthorGroup><Abstract ID="Abs1" Language="En"><Heading>Summary</Heading><Para>Mutants of human immunodeficiency virus type 1 (HIV-1) and type 2 (HIV-2), which have been shown to be infectious in established cell lines, were tested for ability to replicate and induce syncytium formation in human peripheral blood mononuclear cells (PBMC). The<Emphasis Type="Italic">vpu</Emphasis> mutant of HIV-1 showed depressed kinetics of replication in an established T cell line, as reported previously, but in PBMC, its replication was similar to that of the wild type virus. The<Emphasis Type="Italic">vpx</Emphasis> gene of HIV-2 was required for efficient virus propagation in PBMC, but not in an established T cell line, as previously reported. However, the growth rates of the<Emphasis Type="Italic">vpx</Emphasis> mutant in PBMC preparations from two individuals were different. The results of experiments on infection of PBMC with the<Emphasis Type="Italic">vif</Emphasis> and<Emphasis Type="Italic">vpr</Emphasis> mutants of HIV-1 and HIV-2 were essentially consistent with previous results of infection of established T cell lines. No negative effect of the<Emphasis Type="Italic">nef</Emphasis> gene products of HIV-1 and HIV-2 was observed. The abilities of the wild type virus and the mutants of HIV-1 to induce syncytium formation in both PBMC and established cell lines were similar. In contrast, neither the wild type nor any of the mutants of HIV-2 induced syncytium formation in PBMC. These results suggest that the functions of some genes can be detected only in mixed populations or primary cells such as PBMC. Studies on the roles of these genes in PBMC may provide a better understanding of their functions in vivo.</Para></Abstract></ArticleHeader><NoBody></NoBody></Article></Issue></Volume></Journal></Publisher></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Biological characterization of human immunodeficiency virus type 1 and type 2 mutants in human peripheral blood mononuclear cells</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Biological characterization of human immunodeficiency virus type 1 and type 2 mutants in human peripheral blood mononuclear cells</title></titleInfo><name type="personal"><namePart type="given">H.</namePart><namePart type="family">Akari</namePart><affiliation>Research Center for Immunodeficiency Virus, Institute for Virus Research, Kyoto University, Kyoto</affiliation><affiliation>Department of Veterinary Medicine, Faculty of Agriculture, Miyazaki University, Miyazaki, Japan</affiliation><affiliation>Department of Biodefence and Medical Virology, Kumamoto University Medical School, 860, Kumamoto, Japan</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J.</namePart><namePart type="family">Sakuragi</namePart><affiliation>Research Center for Immunodeficiency Virus, Institute for Virus Research, Kyoto University, Kyoto</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Y.</namePart><namePart type="family">Takebe</namePart><affiliation>AIDS Research Center, National Institute of Health, Tokyo</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">K.</namePart><namePart type="family">Tomonaga</namePart><affiliation>Research Center for Immunodeficiency Virus, Institute for Virus Research, Kyoto University, Kyoto</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M.</namePart><namePart type="family">Kawamura</namePart><affiliation>Research Center for Immunodeficiency Virus, Institute for Virus Research, Kyoto University, Kyoto</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M.</namePart><namePart type="family">Fukasawa</namePart><affiliation>Research Center for Immunodeficiency Virus, Institute for Virus Research, Kyoto University, Kyoto</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">T.</namePart><namePart type="family">Miura</namePart><affiliation>Research Center for Immunodeficiency Virus, Institute for Virus Research, Kyoto University, Kyoto</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">T.</namePart><namePart type="family">Shinjo</namePart><affiliation>Department of Veterinary Medicine, Faculty of Agriculture, Miyazaki University, Miyazaki, Japan</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M.</namePart><namePart type="family">Hayami</namePart><affiliation>Research Center for Immunodeficiency Virus, Institute for Virus Research, Kyoto University, Kyoto</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="OriginalPaper"></genre><originInfo><publisher>Springer-Verlag</publisher><place><placeTerm type="text">Vienna</placeTerm></place><dateCreated encoding="w3cdtf">1991-05-31</dateCreated><dateIssued encoding="w3cdtf">1992-03-01</dateIssued><copyrightDate encoding="w3cdtf">1992</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType></physicalDescription><abstract lang="en">Summary: Mutants of human immunodeficiency virus type 1 (HIV-1) and type 2 (HIV-2), which have been shown to be infectious in established cell lines, were tested for ability to replicate and induce syncytium formation in human peripheral blood mononuclear cells (PBMC). Thevpu mutant of HIV-1 showed depressed kinetics of replication in an established T cell line, as reported previously, but in PBMC, its replication was similar to that of the wild type virus. Thevpx gene of HIV-2 was required for efficient virus propagation in PBMC, but not in an established T cell line, as previously reported. However, the growth rates of thevpx mutant in PBMC preparations from two individuals were different. The results of experiments on infection of PBMC with thevif andvpr mutants of HIV-1 and HIV-2 were essentially consistent with previous results of infection of established T cell lines. No negative effect of thenef gene products of HIV-1 and HIV-2 was observed. The abilities of the wild type virus and the mutants of HIV-1 to induce syncytium formation in both PBMC and established cell lines were similar. In contrast, neither the wild type nor any of the mutants of HIV-2 induced syncytium formation in PBMC. These results suggest that the functions of some genes can be detected only in mixed populations or primary cells such as PBMC. Studies on the roles of these genes in PBMC may provide a better understanding of their functions in vivo.</abstract><note>Original Papers</note><relatedItem type="host"><titleInfo><title>Archives of Virology</title></titleInfo><titleInfo type="abbreviated"><title>Archives of Virology</title></titleInfo><genre type="journal" displayLabel="Archive Journal"></genre><originInfo><dateIssued encoding="w3cdtf">1992-03-01</dateIssued><copyrightDate encoding="w3cdtf">1992</copyrightDate></originInfo><subject><genre>Biomedicine</genre><topic>Medical Microbiology</topic><topic>Virology</topic><topic>Infectious Diseases</topic></subject><identifier type="ISSN">0304-8608</identifier><identifier type="eISSN">1432-8798</identifier><identifier type="JournalID">705</identifier><identifier type="IssueArticleCount">22</identifier><identifier type="VolumeIssueCount">4</identifier><part><date>1992</date><detail type="volume"><number>123</number><caption>vol.</caption></detail><detail type="issue"><number>1-2</number><caption>no.</caption></detail><extent unit="pages"><start>157</start><end>167</end></extent></part><recordInfo><recordOrigin>Springer-Verlag, 1992</recordOrigin></recordInfo></relatedItem><identifier type="istex">4D4EEBB15A120CC49AA38C1FAE57CE835767985A</identifier><identifier type="DOI">10.1007/BF01317146</identifier><identifier type="ArticleID">BF01317146</identifier><identifier type="ArticleID">Art14</identifier><accessCondition type="use and reproduction" contentType="copyright">Springer-Verlag, 1992</accessCondition><recordInfo><recordContentSource>SPRINGER</recordContentSource><recordOrigin>Springer-Verlag, 1992</recordOrigin></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/SidaGhanaV1/Data/Istex/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000083 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Istex/Corpus/biblio.hfd -nk 000083 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Sante
|area= SidaGhanaV1
|flux= Istex
|étape= Corpus
|type= RBID
|clé= ISTEX:4D4EEBB15A120CC49AA38C1FAE57CE835767985A
|texte= Biological characterization of human immunodeficiency virus type 1 and type 2 mutants in human peripheral blood mononuclear cells
}}
| This area was generated with Dilib version V0.6.31. |  |