Environmental enrichment during adolescence regulates gene expression in the striatum of mice.
Identifieur interne : 000875 ( Ncbi/Checkpoint ); précédent : 000874; suivant : 000876Environmental enrichment during adolescence regulates gene expression in the striatum of mice.
Auteurs : Nathalie Thiriet [France] ; Lahouari Amar ; Xavier Toussay ; Virginie Lardeux ; Bruce Ladenheim ; Kevin G. Becker ; Jean Lud Cadet ; Marcello Solinas ; Mohamed JaberSource :
- Brain research [ 0006-8993 ] ; 2008.
English descriptors
- KwdEn :
- Animals, Cell Differentiation (genetics), Cell Proliferation, Cluster Analysis, Corpus Striatum (cytology), Corpus Striatum (metabolism), Environment, Gene Expression Profiling (methods), Gene Expression Regulation, Developmental (genetics), Gene Expression Regulation, Developmental (physiology), Male, Mice, Mice, Inbred C57BL, Oligonucleotide Array Sequence Analysis (methods), RNA, Messenger (metabolism), Signal Transduction (genetics).
- MESH :
- chemical , metabolism : RNA, Messenger.
- cytology : Corpus Striatum.
- genetics : Cell Differentiation, Gene Expression Regulation, Developmental, Signal Transduction.
- metabolism : Corpus Striatum.
- methods : Gene Expression Profiling, Oligonucleotide Array Sequence Analysis.
- physiology : Gene Expression Regulation, Developmental.
- Animals, Cell Proliferation, Cluster Analysis, Environment, Male, Mice, Mice, Inbred C57BL.
Abstract
We have previously shown that environmental enrichment decreases the activating and rewarding effects of the psychostimulant cocaine and increases resistance to the neurotoxic effect of the Parkinson-inducing drug MPTP. These effects were accompanied by an increase in the striatal expression of the neurotrophin BDNF, an increase in the striatal levels of delta-Fos B and by a decrease in striatal levels of the dopamine transporter, the main molecular target for cocaine and MPTP. Here, we used cDNA arrays to investigate the effects of rearing mice in enriched environments from weaning to adulthood on the profile of expression of genes in the striatum focusing on genes involved in intracellular signalling and functioning. We found that mice reared in an enriched environment show several alterations in the levels of mRNA coding for proteins involved in cell proliferation, cell differentiation, signal transduction, transcription and translation, cell structure and metabolism. Several of these findings were further confirmed by real-time quantitative PCR and, in the case of protein kinase C lambda, also by western blot. These findings are the first description of alterations in striatal gene expression by an enriched environment. The striatal gene expression regulation by environment that we report here may play a role in the resistance to the effects of drugs of abuse and dopaminergic neurotoxins previously reported.
DOI: 10.1016/j.brainres.2008.05.030
PubMed: 18585688
Affiliations:
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These effects were accompanied by an increase in the striatal expression of the neurotrophin BDNF, an increase in the striatal levels of delta-Fos B and by a decrease in striatal levels of the dopamine transporter, the main molecular target for cocaine and MPTP. Here, we used cDNA arrays to investigate the effects of rearing mice in enriched environments from weaning to adulthood on the profile of expression of genes in the striatum focusing on genes involved in intracellular signalling and functioning. We found that mice reared in an enriched environment show several alterations in the levels of mRNA coding for proteins involved in cell proliferation, cell differentiation, signal transduction, transcription and translation, cell structure and metabolism. Several of these findings were further confirmed by real-time quantitative PCR and, in the case of protein kinase C lambda, also by western blot. These findings are the first description of alterations in striatal gene expression by an enriched environment. The striatal gene expression regulation by environment that we report here may play a role in the resistance to the effects of drugs of abuse and dopaminergic neurotoxins previously reported.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Nouvelle-Aquitaine</li><li>Poitou-Charentes</li></region><settlement><li>Poitiers</li></settlement></list><tree><noCountry><name sortKey="Amar, Lahouari" sort="Amar, Lahouari" uniqKey="Amar L" first="Lahouari" last="Amar">Lahouari Amar</name><name sortKey="Becker, Kevin G" sort="Becker, Kevin G" uniqKey="Becker K" first="Kevin G" last="Becker">Kevin G. Becker</name><name sortKey="Cadet, Jean Lud" sort="Cadet, Jean Lud" uniqKey="Cadet J" first="Jean Lud" last="Cadet">Jean Lud Cadet</name><name sortKey="Jaber, Mohamed" sort="Jaber, Mohamed" uniqKey="Jaber M" first="Mohamed" last="Jaber">Mohamed Jaber</name><name sortKey="Ladenheim, Bruce" sort="Ladenheim, Bruce" uniqKey="Ladenheim B" first="Bruce" last="Ladenheim">Bruce Ladenheim</name><name sortKey="Lardeux, Virginie" sort="Lardeux, Virginie" uniqKey="Lardeux V" first="Virginie" last="Lardeux">Virginie Lardeux</name><name sortKey="Solinas, Marcello" sort="Solinas, Marcello" uniqKey="Solinas M" first="Marcello" last="Solinas">Marcello Solinas</name><name sortKey="Toussay, Xavier" sort="Toussay, Xavier" uniqKey="Toussay X" first="Xavier" last="Toussay">Xavier Toussay</name></noCountry><country name="France"><region name="Nouvelle-Aquitaine"><name sortKey="Thiriet, Nathalie" sort="Thiriet, Nathalie" uniqKey="Thiriet N" first="Nathalie" last="Thiriet">Nathalie Thiriet</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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