Does neuronal expression of GDNF effectively protect dopaminergic neurons in a rat model of Parkinson's disease?
Identifieur interne : 002881 ( Main/Merge ); précédent : 002880; suivant : 002882Does neuronal expression of GDNF effectively protect dopaminergic neurons in a rat model of Parkinson's disease?
Auteurs : N A Do Thi [France] ; P. Saillour ; L. Ferrero ; T. Paunio ; J. MalletSource :
- Gene therapy [ 0969-7128 ] ; 2007.
English descriptors
- KwdEn :
- Adenoviridae (genetics), Animals, Astrocytes (metabolism), Cells, Cultured, Corpus Striatum (metabolism), Dopamine (metabolism), Female, Gene Expression, Genetic Therapy (methods), Genetic Vectors (administration & dosage), Glial Cell Line-Derived Neurotrophic Factor (genetics), Glial Cell Line-Derived Neurotrophic Factor (metabolism), Mesencephalon (metabolism), Neurons (metabolism), Parkinson Disease (metabolism), Phosphopyruvate Hydratase (genetics), Promoter Regions, Genetic, Rats, Rats, Sprague-Dawley, Substantia Nigra (metabolism), Transduction, Genetic (methods), Transgenes.
- MESH :
- chemical , genetics : Glial Cell Line-Derived Neurotrophic Factor, Phosphopyruvate Hydratase.
- chemical , metabolism : Dopamine, Glial Cell Line-Derived Neurotrophic Factor.
- administration & dosage : Genetic Vectors.
- genetics : Adenoviridae.
- metabolism : Astrocytes, Corpus Striatum, Mesencephalon, Neurons, Parkinson Disease, Substantia Nigra.
- methods : Genetic Therapy, Transduction, Genetic.
- Animals, Cells, Cultured, Female, Gene Expression, Promoter Regions, Genetic, Rats, Rats, Sprague-Dawley, Transgenes.
Abstract
The transfer of the Glial cell line-derived neurotrophic factor (GDNF) gene to the central nervous system by a recombinant adenoviral vector (Ad) was studied. We constructed the adenovirus vector Ad-NSE-GDNF from which the E1, E3/E4 regions of Ad5 have been deleted and in which the GDNF gene was under the control of a neuron-specific enolase (NSE) promoter. The vector was injected into the striatum of a rat model of Parkinson's disease. We found that (i) the NSE promoter can restrict transgene expression in neurons; (ii) Ad-NSE-GDNF significantly protected dopaminergic (DA) neurons in the substantia nigra (SN) but did not reverse the impairments of amphetamine-induced rotational behavior in lesioned rats.
Url:
DOI: 10.1038/sj.gt.3302844
PubMed: 17093508
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pubmed:17093508Le document en format XML
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We constructed the adenovirus vector Ad-NSE-GDNF from which the E1, E3/E4 regions of Ad5 have been deleted and in which the GDNF gene was under the control of a neuron-specific enolase (NSE) promoter. The vector was injected into the striatum of a rat model of Parkinson's disease. We found that (i) the NSE promoter can restrict transgene expression in neurons; (ii) Ad-NSE-GDNF significantly protected dopaminergic (DA) neurons in the substantia nigra (SN) but did not reverse the impairments of amphetamine-induced rotational behavior in lesioned rats.</div></front></TEI></HAL><PubMed><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Does neuronal expression of GDNF effectively protect dopaminergic neurons in a rat model of Parkinson's disease?</title><author><name sortKey="Do Thi, N A" sort="Do Thi, N A" uniqKey="Do Thi N" first="N A" last="Do Thi">N A Do Thi</name><affiliation wicri:level="3"><nlm:affiliation>Laboratoire de Genetique Moleculaire de la Neurotransmission et des Processus Neurodegeneratifs, CNRS, UMR 7091, Bat. CERVI, Hopital Pitie-Salpetriere, Paris, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Genetique Moleculaire de la Neurotransmission et des Processus Neurodegeneratifs, CNRS, UMR 7091, Bat. CERVI, Hopital Pitie-Salpetriere, Paris</wicri:regionArea><placeName><region type="region">Île-de-France</region><region type="old region">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation></author><author><name sortKey="Saillour, P" sort="Saillour, P" uniqKey="Saillour P" first="P" last="Saillour">P. Saillour</name></author><author><name sortKey="Ferrero, L" sort="Ferrero, L" uniqKey="Ferrero L" first="L" last="Ferrero">L. Ferrero</name></author><author><name sortKey="Paunio, T" sort="Paunio, T" uniqKey="Paunio T" first="T" last="Paunio">T. Paunio</name></author><author><name sortKey="Mallet, J" sort="Mallet, J" uniqKey="Mallet J" first="J" last="Mallet">J. 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CERVI, Hopital Pitie-Salpetriere, Paris, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Genetique Moleculaire de la Neurotransmission et des Processus Neurodegeneratifs, CNRS, UMR 7091, Bat. CERVI, Hopital Pitie-Salpetriere, Paris</wicri:regionArea><placeName><region type="region">Île-de-France</region><region type="old region">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation></author><author><name sortKey="Saillour, P" sort="Saillour, P" uniqKey="Saillour P" first="P" last="Saillour">P. Saillour</name></author><author><name sortKey="Ferrero, L" sort="Ferrero, L" uniqKey="Ferrero L" first="L" last="Ferrero">L. Ferrero</name></author><author><name sortKey="Paunio, T" sort="Paunio, T" uniqKey="Paunio T" first="T" last="Paunio">T. Paunio</name></author><author><name sortKey="Mallet, J" sort="Mallet, J" uniqKey="Mallet J" first="J" last="Mallet">J. Mallet</name></author></analytic><series><title level="j">Gene therapy</title><idno type="ISSN">0969-7128</idno><imprint><date when="2007" type="published">2007</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adenoviridae (genetics)</term><term>Animals</term><term>Astrocytes (metabolism)</term><term>Cells, Cultured</term><term>Corpus Striatum (metabolism)</term><term>Dopamine (metabolism)</term><term>Female</term><term>Gene Expression</term><term>Genetic Therapy (methods)</term><term>Genetic Vectors (administration & dosage)</term><term>Glial Cell Line-Derived Neurotrophic Factor (genetics)</term><term>Glial Cell Line-Derived Neurotrophic Factor (metabolism)</term><term>Mesencephalon (metabolism)</term><term>Neurons (metabolism)</term><term>Parkinson Disease (metabolism)</term><term>Phosphopyruvate Hydratase (genetics)</term><term>Promoter Regions, Genetic</term><term>Rats</term><term>Rats, Sprague-Dawley</term><term>Substantia Nigra (metabolism)</term><term>Transduction, Genetic (methods)</term><term>Transgenes</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Glial Cell Line-Derived Neurotrophic Factor</term><term>Phosphopyruvate Hydratase</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Dopamine</term><term>Glial Cell Line-Derived Neurotrophic Factor</term></keywords><keywords scheme="MESH" qualifier="administration & dosage" xml:lang="en"><term>Genetic Vectors</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Adenoviridae</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Astrocytes</term><term>Corpus Striatum</term><term>Mesencephalon</term><term>Neurons</term><term>Parkinson Disease</term><term>Substantia Nigra</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Genetic Therapy</term><term>Transduction, Genetic</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cells, Cultured</term><term>Female</term><term>Gene Expression</term><term>Promoter Regions, Genetic</term><term>Rats</term><term>Rats, Sprague-Dawley</term><term>Transgenes</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The transfer of the Glial cell line-derived neurotrophic factor (GDNF) gene to the central nervous system by a recombinant adenoviral vector (Ad) was studied. We constructed the adenovirus vector Ad-NSE-GDNF from which the E1, E3/E4 regions of Ad5 have been deleted and in which the GDNF gene was under the control of a neuron-specific enolase (NSE) promoter. The vector was injected into the striatum of a rat model of Parkinson's disease. We found that (i) the NSE promoter can restrict transgene expression in neurons; (ii) Ad-NSE-GDNF significantly protected dopaminergic (DA) neurons in the substantia nigra (SN) but did not reverse the impairments of amphetamine-induced rotational behavior in lesioned rats.</div></front></TEI></PubMed></double></record>Pour manipuler ce document sous Unix (Dilib)
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