Optimizing Western Blots for the Detection of Endogenous α-Synuclein in the Enteric Nervous System.
Identifieur interne : 000555 ( Main/Exploration ); précédent : 000554; suivant : 000556Optimizing Western Blots for the Detection of Endogenous α-Synuclein in the Enteric Nervous System.
Auteurs : Cécile Preterre [France] ; Anne-Gaëlle Corbillé [France] ; Gaëlle Balloy [France] ; Franck Letournel [France] ; Michel Neunlist [France] ; Pascal Derkinderen [France]Source :
- Journal of Parkinson's disease [ 1877-718X ] ; 2015.
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : alpha-Synuclein.
- chemical , metabolism : Biomarkers.
- diagnosis : Parkinson Disease.
- innervation : Intestine, Small.
- metabolism : Enteric Nervous System, Intestine, Small.
- methods : Blotting, Western.
- standards : Blotting, Western.
- Animals, Humans, Rats.
- mix :
Abstract
Alpha-synuclein containing inclusions in neurons, the characteristic pathological lesions of Parkinson's disease (PD), are not limited to the central nervous system, but also affect the enteric nervous system (ENS). This suggests that the ENS offer some potential as a surrogate of central nervous system pathology and that it may represent an original source of biomarkers for PD. However, the usefulness of α-synuclein detection in gastrointestinal biopsies as a biomarker for PD is still unclear, as the different immunohistochemical methods employed to date have led to conflicting results.
Url:
DOI: 10.3233/JPD-150670
PubMed: 26599299
Affiliations:
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Le document en format XML
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xml:lang="fr">France</country><wicri:regionArea>Inserm, U913, Nantes, F-44093</wicri:regionArea><placeName><region type="region" nuts="2">Pays de la Loire</region></placeName></affiliation></author><author><name sortKey="Corbille, Anne Gaelle" sort="Corbille, Anne Gaelle" uniqKey="Corbille A" first="Anne-Gaëlle" last="Corbillé">Anne-Gaëlle Corbillé</name><affiliation wicri:level="3"><nlm:affiliation>Inserm, U913, Nantes, F-44093, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Inserm, U913, Nantes, F-44093</wicri:regionArea><placeName><region type="region" nuts="2">Pays de la Loire</region></placeName></affiliation></author><author><name sortKey="Balloy, Gaelle" sort="Balloy, Gaelle" uniqKey="Balloy G" first="Gaëlle" last="Balloy">Gaëlle Balloy</name><affiliation wicri:level="3"><nlm:affiliation>Inserm, U913, Nantes, F-44093, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Inserm, U913, Nantes, F-44093</wicri:regionArea><placeName><region type="region" nuts="2">Pays de la Loire</region></placeName></affiliation></author><author><name sortKey="Letournel, Franck" sort="Letournel, Franck" uniqKey="Letournel F" first="Franck" last="Letournel">Franck Letournel</name><affiliation wicri:level="3"><nlm:affiliation>CHU Angers, Neurobiology and Neuropathology Laboratory, Angers F-49033, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>CHU Angers, Neurobiology and Neuropathology Laboratory, Angers F-49033</wicri:regionArea><placeName><region type="region" nuts="2">Pays de la Loire</region></placeName></affiliation></author><author><name sortKey="Neunlist, Michel" sort="Neunlist, Michel" uniqKey="Neunlist M" first="Michel" last="Neunlist">Michel Neunlist</name><affiliation wicri:level="3"><nlm:affiliation>Inserm, U913, Nantes, F-44093, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Inserm, U913, Nantes, F-44093</wicri:regionArea><placeName><region type="region" nuts="2">Pays de la Loire</region></placeName></affiliation></author><author><name sortKey="Derkinderen, Pascal" sort="Derkinderen, Pascal" uniqKey="Derkinderen P" first="Pascal" last="Derkinderen">Pascal Derkinderen</name><affiliation wicri:level="3"><nlm:affiliation>Inserm, U913, Nantes, F-44093, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Inserm, U913, Nantes, F-44093</wicri:regionArea><placeName><region type="region" nuts="2">Pays de la Loire</region></placeName></affiliation></author></analytic><series><title level="j">Journal of Parkinson's disease</title><idno type="eISSN">1877-718X</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Biomarkers (metabolism)</term><term>Blotting, Western (methods)</term><term>Blotting, Western (standards)</term><term>Enteric Nervous System (metabolism)</term><term>Humans</term><term>Intestine, Small (innervation)</term><term>Intestine, Small (metabolism)</term><term>Parkinson Disease (diagnosis)</term><term>Rats</term><term>alpha-Synuclein (analysis)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>alpha-Synuclein</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Biomarkers</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Parkinson Disease</term></keywords><keywords scheme="MESH" qualifier="innervation" xml:lang="en"><term>Intestine, Small</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Enteric Nervous System</term><term>Intestine, Small</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Blotting, Western</term></keywords><keywords scheme="MESH" qualifier="standards" xml:lang="en"><term>Blotting, Western</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Humans</term><term>Rats</term></keywords><keywords scheme="mix" xml:lang="en"><term>Alpha-synuclein</term><term>Parkinson’s disease</term><term>Western Blot</term><term>enteric nervous system</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Alpha-synuclein containing inclusions in neurons, the characteristic pathological lesions of Parkinson's disease (PD), are not limited to the central nervous system, but also affect the enteric nervous system (ENS). This suggests that the ENS offer some potential as a surrogate of central nervous system pathology and that it may represent an original source of biomarkers for PD. However, the usefulness of α-synuclein detection in gastrointestinal biopsies as a biomarker for PD is still unclear, as the different immunohistochemical methods employed to date have led to conflicting results.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Pays de la Loire</li></region></list><tree><country name="France"><region name="Pays de la Loire"><name sortKey="Preterre, Cecile" sort="Preterre, Cecile" uniqKey="Preterre C" first="Cécile" last="Preterre">Cécile Preterre</name></region><name sortKey="Balloy, Gaelle" sort="Balloy, Gaelle" uniqKey="Balloy G" first="Gaëlle" last="Balloy">Gaëlle Balloy</name><name sortKey="Corbille, Anne Gaelle" sort="Corbille, Anne Gaelle" uniqKey="Corbille A" first="Anne-Gaëlle" last="Corbillé">Anne-Gaëlle Corbillé</name><name sortKey="Derkinderen, Pascal" sort="Derkinderen, Pascal" uniqKey="Derkinderen P" first="Pascal" last="Derkinderen">Pascal Derkinderen</name><name sortKey="Letournel, Franck" sort="Letournel, Franck" uniqKey="Letournel F" first="Franck" last="Letournel">Franck Letournel</name><name sortKey="Neunlist, Michel" sort="Neunlist, Michel" uniqKey="Neunlist M" first="Michel" last="Neunlist">Michel Neunlist</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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