ParkinsonFranceV1, Main, Exploration, bibRecord, 003B91

Extracellular toxicity of 6-hydroxydopamine on PC12 cells

Identifieur interne : 003B91 ( Main/Exploration ); précédent : 003B90; suivant : 003B92

Extracellular toxicity of 6-hydroxydopamine on PC12 cells

Auteurs : D. Blum [Belgique, France] ; S. Torch [France] ; M.-F. Nissou [France] ; A.-L. Benabid [France] ; J.-M. Verna [France]

Source :

Neuroscience letters [ 0304-3940 ] ; 2000.

RBID : Pascal:00-0242455

Descripteurs français

Pascal (Inist)
- Extracellulaire, Oxidopamine, Toxicité, Lignée cellulaire, Neurone, In vitro, Stress oxydatif, Parkinson maladie, Mort cellulaire, Lignée PC12.

English descriptors

KwdEn :
- Cell death, Cell line, Extracellular, In vitro, Neuron, Oxidative stress, Oxidopamine, Parkinson disease, Toxicity.

Abstract

6-hydroxydopamine (6-OHDA) is usually thought to cross cell membrane through dopamine uptake transporters, to inhibit mitochondrial respiration and to generate intracellular reactive oxygen species. In this study, we show that the anti-oxidants catalase, glutathion and N-acetyl-cystein are able to reverse the toxic effects of 6-OHDA. These two latter compounds considerably slow down 6-OHDA oxidation in a cell free system suggesting a direct chemical interaction with the neurotoxin. Moreover, desipramine does not protect PC12 cells and 6-OHDA is also strongly toxic towards non-catecholaminergic C6 and NIH3T3 cells. These results thus suggest that 6-OHDA toxicity on PC12 cells mainly involves an extracellular process.

Affiliations:

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Le document en format XML

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<front><div type="abstract" xml:lang="en">6-hydroxydopamine (6-OHDA) is usually thought to cross cell membrane through dopamine uptake transporters, to inhibit mitochondrial respiration and to generate intracellular reactive oxygen species. In this study, we show that the anti-oxidants catalase, glutathion and N-acetyl-cystein are able to reverse the toxic effects of 6-OHDA. These two latter compounds considerably slow down 6-OHDA oxidation in a cell free system suggesting a direct chemical interaction with the neurotoxin. Moreover, desipramine does not protect PC12 cells and 6-OHDA is also strongly toxic towards non-catecholaminergic C6 and NIH3T3 cells. These results thus suggest that 6-OHDA toxicity on PC12 cells mainly involves an extracellular process.</div>
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	La maladie de Parkinson en France (serveur d'exploration)
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La maladie de Parkinson en France (serveur d'exploration)

Extracellular toxicity of 6-hydroxydopamine on PC12 cells

Extracellular toxicity of 6-hydroxydopamine on PC12 cells

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri