Increasing the Detection Limit of the Parkinson Disorder through a Specific Surface Chemistry Applied onto Inner Surface of the Titration Well.
Identifieur interne : 001120 ( Main/Exploration ); précédent : 001119; suivant : 001121Increasing the Detection Limit of the Parkinson Disorder through a Specific Surface Chemistry Applied onto Inner Surface of the Titration Well.
Auteurs : Caroline Mille [France] ; Dominique Debarnot [France] ; Willy Zorzi [Belgique] ; Benaïssa El Moualij [Belgique] ; Arnaud Coudreuse [France] ; Gilbert Legeay [France] ; Isabelle Quadrio [France] ; Armand Perret-Liaudet [France] ; Fabienne Poncin-Epaillard [France]Source :
- Journal of functional biomaterials ; 2012.
Abstract
The main objective of this paper was to illustrate the enhancement of the sensitivity of ELISA titration for neurodegenerative proteins by reducing nonspecific adsorptions that could lead to false positives. This goal was obtained thanks to the association of plasma and wet chemistries applied to the inner surface of the titration well. The polypropylene surface was plasma-activated and then, dip-coated with different amphiphilic molecules. These molecules have more or less long hydrocarbon chains and may be charged. The modified surfaces were characterized in terms of hydrophilic-phobic character, surface chemical groups and topography. Finally, the coated wells were tested during the ELISA titration of the specific antibody capture of the α-synuclein protein. The highest sensitivity is obtained with polar (Θ = 35°), negatively charged and smooth inner surface.
DOI: 10.3390/jfb3020298
PubMed: 24955533
Affiliations:
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4000</wicri:noRegion></affiliation></author><author><name sortKey="El Moualij, Benaissa" sort="El Moualij, Benaissa" uniqKey="El Moualij B" first="Benaïssa" last="El Moualij">Benaïssa El Moualij</name><affiliation wicri:level="1"><nlm:affiliation>Centre de Recherche sur les Protéines Prion, Institut de Pharmacie, B36, No. 1 avenue de l'Hôpital, Liège 4000, Belgium. b.elmoualij@ulg.ac.be.</nlm:affiliation><country xml:lang="fr">Belgique</country><wicri:regionArea>Centre de Recherche sur les Protéines Prion, Institut de Pharmacie, B36, No. 1 avenue de l'Hôpital, Liège 4000</wicri:regionArea><wicri:noRegion>Liège 4000</wicri:noRegion></affiliation></author><author><name sortKey="Coudreuse, Arnaud" sort="Coudreuse, Arnaud" uniqKey="Coudreuse A" first="Arnaud" last="Coudreuse">Arnaud Coudreuse</name><affiliation wicri:level="3"><nlm:affiliation>CTTM, 20 rue Thalès de Milet, Le Mans 72000, France. acoudreuse@cttm-lemans.com.</nlm:affiliation><country 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bd Pinel, Bron cedex 69677, France. isabelle.quadrio@chu-lyon.fr.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Centre Mémoire de Ressources et Recherche, Service de Neurobiologie, Groupement Hospitalier Est du CHU, 56 bd Pinel, Bron cedex 69677</wicri:regionArea><placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region><region type="old region" nuts="2">Rhône-Alpes</region></placeName></affiliation></author><author><name sortKey="Perret Liaudet, Armand" sort="Perret Liaudet, Armand" uniqKey="Perret Liaudet A" first="Armand" last="Perret-Liaudet">Armand Perret-Liaudet</name><affiliation wicri:level="3"><nlm:affiliation>Centre Mémoire de Ressources et Recherche, Service de Neurobiologie, Groupement Hospitalier Est du CHU, 56 bd Pinel, Bron cedex 69677, France. armand.perret-liaudet@chu-lyon.fr.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Centre Mémoire de Ressources et Recherche, Service de Neurobiologie, Groupement Hospitalier Est du CHU, 56 bd Pinel, Bron cedex 69677</wicri:regionArea><placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region><region type="old region" nuts="2">Rhône-Alpes</region></placeName></affiliation></author><author><name sortKey="Poncin Epaillard, Fabienne" sort="Poncin Epaillard, Fabienne" uniqKey="Poncin Epaillard F" first="Fabienne" last="Poncin-Epaillard">Fabienne Poncin-Epaillard</name><affiliation wicri:level="3"><nlm:affiliation>Département Polymères, Institut des Molécules et Matériaux du Mans, Colloïdes et Interfaces, Lunam Université, UMR Université du Maine-CNRS No. 6283, Avenue Olivier Messiaen, Le Mans Cedex 72085, France. fabienne.poncin-epaillard@univ-lemans.fr.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Département Polymères, Institut des Molécules et Matériaux du Mans, Colloïdes et Interfaces, Lunam Université, UMR Université du Maine-CNRS No. 6283, Avenue Olivier Messiaen, Le Mans Cedex 72085</wicri:regionArea><placeName><region type="region" nuts="2">Pays de la Loire</region></placeName></affiliation></author></analytic><series><title level="j">Journal of functional biomaterials</title><imprint><date when="2012" type="published">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The main objective of this paper was to illustrate the enhancement of the sensitivity of ELISA titration for neurodegenerative proteins by reducing nonspecific adsorptions that could lead to false positives. This goal was obtained thanks to the association of plasma and wet chemistries applied to the inner surface of the titration well. The polypropylene surface was plasma-activated and then, dip-coated with different amphiphilic molecules. These molecules have more or less long hydrocarbon chains and may be charged. The modified surfaces were characterized in terms of hydrophilic-phobic character, surface chemical groups and topography. Finally, the coated wells were tested during the ELISA titration of the specific antibody capture of the α-synuclein protein. The highest sensitivity is obtained with polar (Θ = 35°), negatively charged and smooth inner surface.</div></front></TEI><affiliations><list><country><li>Belgique</li><li>France</li></country><region><li>Auvergne-Rhône-Alpes</li><li>Pays de la Loire</li><li>Rhône-Alpes</li></region></list><tree><country name="France"><region name="Pays de la Loire"><name sortKey="Mille, Caroline" sort="Mille, Caroline" uniqKey="Mille C" first="Caroline" last="Mille">Caroline Mille</name></region><name sortKey="Coudreuse, Arnaud" sort="Coudreuse, Arnaud" uniqKey="Coudreuse A" first="Arnaud" last="Coudreuse">Arnaud Coudreuse</name><name sortKey="Debarnot, Dominique" sort="Debarnot, Dominique" uniqKey="Debarnot D" first="Dominique" last="Debarnot">Dominique Debarnot</name><name sortKey="Legeay, Gilbert" sort="Legeay, Gilbert" uniqKey="Legeay G" first="Gilbert" last="Legeay">Gilbert Legeay</name><name sortKey="Perret Liaudet, Armand" sort="Perret Liaudet, Armand" uniqKey="Perret Liaudet A" first="Armand" last="Perret-Liaudet">Armand Perret-Liaudet</name><name sortKey="Poncin Epaillard, Fabienne" sort="Poncin Epaillard, Fabienne" uniqKey="Poncin Epaillard F" first="Fabienne" last="Poncin-Epaillard">Fabienne Poncin-Epaillard</name><name sortKey="Quadrio, Isabelle" sort="Quadrio, Isabelle" uniqKey="Quadrio I" first="Isabelle" last="Quadrio">Isabelle Quadrio</name></country><country name="Belgique"><noRegion><name sortKey="Zorzi, Willy" sort="Zorzi, Willy" uniqKey="Zorzi W" first="Willy" last="Zorzi">Willy Zorzi</name></noRegion><name sortKey="El Moualij, Benaissa" sort="El Moualij, Benaissa" uniqKey="El Moualij B" first="Benaïssa" last="El Moualij">Benaïssa El Moualij</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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