Axon diodes for the reconstruction of oriented neuronal networks in microfluidic chambers.
Identifieur interne : 001616 ( Main/Curation ); précédent : 001615; suivant : 001617Axon diodes for the reconstruction of oriented neuronal networks in microfluidic chambers.
Auteurs : Jean-Michel Peyrin [France] ; Bérangère Deleglise ; Laure Saias ; Maéva Vignes ; Paul Gougis ; Sebastien Magnifico ; Sandrine Betuing ; Mathéa Pietri ; Jocelyne Caboche ; Peter Vanhoutte ; Jean-Louis Viovy ; Bernard BruggSource :
- Lab on a chip [ 1473-0189 ] ; 2011.
English descriptors
- KwdEn :
- Aniline Compounds (chemistry), Animals, Axons (physiology), Calcium (metabolism), Cell Differentiation, Cells, Cultured, Extracellular Signal-Regulated MAP Kinases (metabolism), Mice, Mice, Transgenic, Microfluidic Analytical Techniques, Nerve Net (cytology), Nerve Net (metabolism), Nerve Net (physiology), Neurons (cytology), Neurons (metabolism), Xanthenes (chemistry).
- MESH :
- chemical , chemistry : Aniline Compounds, Xanthenes.
- chemical , metabolism : Calcium, Extracellular Signal-Regulated MAP Kinases.
- cytology : Nerve Net, Neurons.
- metabolism : Nerve Net, Neurons.
- physiology : Axons, Nerve Net.
- Animals, Cell Differentiation, Cells, Cultured, Mice, Mice, Transgenic, Microfluidic Analytical Techniques.
Abstract
Various experimental models are used to study brain development and degeneration. They range from whole animal models, which preserve anatomical structures but strongly limit investigations at the cellular level, to dissociated cell culture systems that allow detailed observation of cell phenotypes but lack the highly ordered physiological neuron connection architecture. We describe here a platform comprising independent cell culture chambers separated by an array of "axonal diodes". This array involves asymmetric micro-channels, imposing unidirectional axon connectivity with 97% selectivity. It allows the construction of complex, oriented neuronal networks not feasible with earlier platforms. Different neuronal subtypes could be co-cultivated for weeks, and sequential seeding of different cell populations reproduced physiological network development. To illustrate possible applications, we created and characterized a cortico-striatal oriented network. Functional synaptic connections were established. The activation of striatal differentiation by cortical axons, and the synchronization of neural activity were demonstrated. Each neuronal population and subcompartment could be chemically addressed individually. The directionality of neural pathways being a key feature of the nervous system organization, the axon diode concept brings in a paradigmatic change in neuronal culture platforms, with potential applications for studying neuronal development, synaptic transmission and neurodegenerative disorder such as Alzheimer and Parkinson diseases at the sub-cellular, cellular and network levels.
DOI: 10.1039/c1lc20014c
PubMed: 21922081
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: Pour aller vers cette notice dans l'étape Curation :000933
- to stream PubMed, to step Curation: Pour aller vers cette notice dans l'étape Curation :000893
- to stream PubMed, to step Checkpoint: Pour aller vers cette notice dans l'étape Curation :000893
- to stream Ncbi, to step Merge: Pour aller vers cette notice dans l'étape Curation :000D79
- to stream Ncbi, to step Curation: Pour aller vers cette notice dans l'étape Curation :000D79
- to stream Ncbi, to step Checkpoint: Pour aller vers cette notice dans l'étape Curation :000D79
- to stream Main, to step Merge: Pour aller vers cette notice dans l'étape Curation :001697
Links to Exploration step
pubmed:21922081Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Axon diodes for the reconstruction of oriented neuronal networks in microfluidic chambers.</title><author><name sortKey="Peyrin, Jean Michel" sort="Peyrin, Jean Michel" uniqKey="Peyrin J" first="Jean-Michel" last="Peyrin">Jean-Michel Peyrin</name><affiliation wicri:level="4"><nlm:affiliation>Laboratoire de Neurobiologie des Processus Adaptatifs, CNRS, UMR7102, Université Pierre et Marie Curie, Paris, France. jean-michel.peyrin@snv.jussieu.fr</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Neurobiologie des Processus Adaptatifs, CNRS, UMR7102, Université Pierre et Marie Curie, Paris</wicri:regionArea><placeName><region type="region">Île-de-France</region><region type="old region">Île-de-France</region><settlement type="city">Paris</settlement></placeName><orgName type="university">Université Pierre-et-Marie-Curie</orgName></affiliation></author><author><name sortKey="Deleglise, Berangere" sort="Deleglise, Berangere" uniqKey="Deleglise B" first="Bérangère" last="Deleglise">Bérangère Deleglise</name></author><author><name sortKey="Saias, Laure" sort="Saias, Laure" uniqKey="Saias L" first="Laure" last="Saias">Laure Saias</name></author><author><name sortKey="Vignes, Maeva" sort="Vignes, Maeva" uniqKey="Vignes M" first="Maéva" last="Vignes">Maéva Vignes</name></author><author><name sortKey="Gougis, Paul" sort="Gougis, Paul" uniqKey="Gougis P" first="Paul" last="Gougis">Paul Gougis</name></author><author><name sortKey="Magnifico, Sebastien" sort="Magnifico, Sebastien" uniqKey="Magnifico S" first="Sebastien" last="Magnifico">Sebastien Magnifico</name></author><author><name sortKey="Betuing, Sandrine" sort="Betuing, Sandrine" uniqKey="Betuing S" first="Sandrine" last="Betuing">Sandrine Betuing</name></author><author><name sortKey="Pietri, Mathea" sort="Pietri, Mathea" uniqKey="Pietri M" first="Mathéa" last="Pietri">Mathéa Pietri</name></author><author><name sortKey="Caboche, Jocelyne" sort="Caboche, Jocelyne" uniqKey="Caboche J" first="Jocelyne" last="Caboche">Jocelyne Caboche</name></author><author><name sortKey="Vanhoutte, Peter" sort="Vanhoutte, Peter" uniqKey="Vanhoutte P" first="Peter" last="Vanhoutte">Peter Vanhoutte</name></author><author><name sortKey="Viovy, Jean Louis" sort="Viovy, Jean Louis" uniqKey="Viovy J" first="Jean-Louis" last="Viovy">Jean-Louis Viovy</name></author><author><name sortKey="Brugg, Bernard" sort="Brugg, Bernard" uniqKey="Brugg B" first="Bernard" last="Brugg">Bernard Brugg</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2011">2011</date><idno type="RBID">pubmed:21922081</idno><idno type="pmid">21922081</idno><idno type="doi">10.1039/c1lc20014c</idno><idno type="wicri:Area/PubMed/Corpus">000933</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000933</idno><idno type="wicri:Area/PubMed/Curation">000893</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000893</idno><idno type="wicri:Area/PubMed/Checkpoint">000893</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000893</idno><idno type="wicri:Area/Ncbi/Merge">000D79</idno><idno type="wicri:Area/Ncbi/Curation">000D79</idno><idno type="wicri:Area/Ncbi/Checkpoint">000D79</idno><idno type="wicri:Area/Main/Merge">001697</idno><idno type="wicri:Area/Main/Curation">001616</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Axon diodes for the reconstruction of oriented neuronal networks in microfluidic chambers.</title><author><name sortKey="Peyrin, Jean Michel" sort="Peyrin, Jean Michel" uniqKey="Peyrin J" first="Jean-Michel" last="Peyrin">Jean-Michel Peyrin</name><affiliation wicri:level="4"><nlm:affiliation>Laboratoire de Neurobiologie des Processus Adaptatifs, CNRS, UMR7102, Université Pierre et Marie Curie, Paris, France. jean-michel.peyrin@snv.jussieu.fr</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Neurobiologie des Processus Adaptatifs, CNRS, UMR7102, Université Pierre et Marie Curie, Paris</wicri:regionArea><placeName><region type="region">Île-de-France</region><region type="old region">Île-de-France</region><settlement type="city">Paris</settlement></placeName><orgName type="university">Université Pierre-et-Marie-Curie</orgName></affiliation></author><author><name sortKey="Deleglise, Berangere" sort="Deleglise, Berangere" uniqKey="Deleglise B" first="Bérangère" last="Deleglise">Bérangère Deleglise</name></author><author><name sortKey="Saias, Laure" sort="Saias, Laure" uniqKey="Saias L" first="Laure" last="Saias">Laure Saias</name></author><author><name sortKey="Vignes, Maeva" sort="Vignes, Maeva" uniqKey="Vignes M" first="Maéva" last="Vignes">Maéva Vignes</name></author><author><name sortKey="Gougis, Paul" sort="Gougis, Paul" uniqKey="Gougis P" first="Paul" last="Gougis">Paul Gougis</name></author><author><name sortKey="Magnifico, Sebastien" sort="Magnifico, Sebastien" uniqKey="Magnifico S" first="Sebastien" last="Magnifico">Sebastien Magnifico</name></author><author><name sortKey="Betuing, Sandrine" sort="Betuing, Sandrine" uniqKey="Betuing S" first="Sandrine" last="Betuing">Sandrine Betuing</name></author><author><name sortKey="Pietri, Mathea" sort="Pietri, Mathea" uniqKey="Pietri M" first="Mathéa" last="Pietri">Mathéa Pietri</name></author><author><name sortKey="Caboche, Jocelyne" sort="Caboche, Jocelyne" uniqKey="Caboche J" first="Jocelyne" last="Caboche">Jocelyne Caboche</name></author><author><name sortKey="Vanhoutte, Peter" sort="Vanhoutte, Peter" uniqKey="Vanhoutte P" first="Peter" last="Vanhoutte">Peter Vanhoutte</name></author><author><name sortKey="Viovy, Jean Louis" sort="Viovy, Jean Louis" uniqKey="Viovy J" first="Jean-Louis" last="Viovy">Jean-Louis Viovy</name></author><author><name sortKey="Brugg, Bernard" sort="Brugg, Bernard" uniqKey="Brugg B" first="Bernard" last="Brugg">Bernard Brugg</name></author></analytic><series><title level="j">Lab on a chip</title><idno type="eISSN">1473-0189</idno><imprint><date when="2011" type="published">2011</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Aniline Compounds (chemistry)</term><term>Animals</term><term>Axons (physiology)</term><term>Calcium (metabolism)</term><term>Cell Differentiation</term><term>Cells, Cultured</term><term>Extracellular Signal-Regulated MAP Kinases (metabolism)</term><term>Mice</term><term>Mice, Transgenic</term><term>Microfluidic Analytical Techniques</term><term>Nerve Net (cytology)</term><term>Nerve Net (metabolism)</term><term>Nerve Net (physiology)</term><term>Neurons (cytology)</term><term>Neurons (metabolism)</term><term>Xanthenes (chemistry)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Aniline Compounds</term><term>Xanthenes</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Calcium</term><term>Extracellular Signal-Regulated MAP Kinases</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Nerve Net</term><term>Neurons</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Nerve Net</term><term>Neurons</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Axons</term><term>Nerve Net</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cell Differentiation</term><term>Cells, Cultured</term><term>Mice</term><term>Mice, Transgenic</term><term>Microfluidic Analytical Techniques</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Various experimental models are used to study brain development and degeneration. They range from whole animal models, which preserve anatomical structures but strongly limit investigations at the cellular level, to dissociated cell culture systems that allow detailed observation of cell phenotypes but lack the highly ordered physiological neuron connection architecture. We describe here a platform comprising independent cell culture chambers separated by an array of "axonal diodes". This array involves asymmetric micro-channels, imposing unidirectional axon connectivity with 97% selectivity. It allows the construction of complex, oriented neuronal networks not feasible with earlier platforms. Different neuronal subtypes could be co-cultivated for weeks, and sequential seeding of different cell populations reproduced physiological network development. To illustrate possible applications, we created and characterized a cortico-striatal oriented network. Functional synaptic connections were established. The activation of striatal differentiation by cortical axons, and the synchronization of neural activity were demonstrated. Each neuronal population and subcompartment could be chemically addressed individually. The directionality of neural pathways being a key feature of the nervous system organization, the axon diode concept brings in a paradigmatic change in neuronal culture platforms, with potential applications for studying neuronal development, synaptic transmission and neurodegenerative disorder such as Alzheimer and Parkinson diseases at the sub-cellular, cellular and network levels.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/ParkinsonFranceV1/Data/Main/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001616 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Curation/biblio.hfd -nk 001616 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Sante
|area= ParkinsonFranceV1
|flux= Main
|étape= Curation
|type= RBID
|clé= pubmed:21922081
|texte= Axon diodes for the reconstruction of oriented neuronal networks in microfluidic chambers.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Curation/RBID.i -Sk "pubmed:21922081" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Curation/biblio.hfd \
| NlmPubMed2Wicri -a ParkinsonFranceV1
| This area was generated with Dilib version V0.6.29. |  |