An Assessment of the Potential of Vicia faba minor in the Storage of the L-Form of 3,4-Dihydroxyphenylalanine
Identifieur interne : 002266 ( Istex/Corpus ); précédent : 002265; suivant : 002267An Assessment of the Potential of Vicia faba minor in the Storage of the L-Form of 3,4-Dihydroxyphenylalanine
Auteurs : F. Larher ; J. Gerard ; D. Gerant-Sauvage ; J. Hamelin ; M. BriensSource :
- Journal of Plant Physiology [ 0176-1617 ] ; 1984.
English descriptors
Abstract
Summary: The uncommon amino acid 3,4-dihydroxyphenylalanine (DOPA) was determined colorimetrically using isonicotinic acid hydrazide (IAH) in the crude extracts obtained from fresh material of Vicia faba minor. DOPA was found to accumulate at concentrations inconsistent with its solubility in water. Other orthodiphenols do not interfere in DOPA determination. The accumulated DOPA was only of the L-form. The DOPA content in the dry material of Vicia faba was significantly lower than that from the corresponding fresh material, suggesting some kind of DOPA oxidation during drying. Subsequent analyses designed to evaluate the actual potential of V. faba minor in the accumulation of DOPA were performed with fresh material.It was observed that the DOPA concentration in the tissues of V. faba minor is reproducible when expressed on a fresh matter weight basis. In young seedlings L-DOPA production is significantly enhanced by light via an increased fresh matter yield. The DOPA content was found to be increased in mature plants grown on media enriched with low concentrations of NaCl (up to 25 mM) that showed a positive effect on their growth. NaCl induced an overproduction of L-DOPA even when growth was restricted by ion excess. On the contrary, the DOPA level dropped in mature plants submitted to water stress. Nodulated plants were found to contain higher content of L-DOPA than the non-nodulated ones grown with NO3- and NH4+. It is suggested that DOPA hyperproduction by Vicia faba minor might be optimized by growing plants in mild saline conditions and by inoculating them with efficient salt tolerant strains of R. leguminosarum.
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DOI: 10.1016/S0176-1617(84)80085-1
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Larher</name><affiliation><mods:affiliation>1) Laboratoire de Biologie végétale, U.E.R. Physique, Chimie, Biologie, Université de Nancy I, B.P. 239, F-54506 Vandoeuvre-les-Nancy Cedex (France)</mods:affiliation></affiliation><affiliation><mods:affiliation>To whom correspondence should be addressed.</mods:affiliation></affiliation></author><author><name sortKey="Gerard, J" sort="Gerard, J" uniqKey="Gerard J" first="J." last="Gerard">J. Gerard</name><affiliation><mods:affiliation>1) Laboratoire de Biologie végétale, U.E.R. Physique, Chimie, Biologie, Université de Nancy I, B.P. 239, F-54506 Vandoeuvre-les-Nancy Cedex (France)</mods:affiliation></affiliation></author><author><name sortKey="Gerant Sauvage, D" sort="Gerant Sauvage, D" uniqKey="Gerant Sauvage D" first="D." last="Gerant-Sauvage">D. Gerant-Sauvage</name><affiliation><mods:affiliation>1) Laboratoire de Biologie végétale, U.E.R. Physique, Chimie, Biologie, Université de Nancy I, B.P. 239, F-54506 Vandoeuvre-les-Nancy Cedex (France)</mods:affiliation></affiliation></author><author><name sortKey="Hamelin, J" sort="Hamelin, J" uniqKey="Hamelin J" first="J." last="Hamelin">J. Hamelin</name><affiliation><mods:affiliation>Groupe de Recherche de Physicochimie structurale, U.E.R. Structure et Propriétés de la matière, Université de Rennes I, Campus de Beaulieu, F-35042 Rennes Cedex (France)</mods:affiliation></affiliation></author><author><name sortKey="Briens, M" sort="Briens, M" uniqKey="Briens M" first="M." last="Briens">M. Briens</name><affiliation><mods:affiliation>Laboratoire de Biologie végétale, U.E.R. Sciences de la vie et de l'environnement, Université de Rennes I, Campus de Beaulieu, F-35042 Rennes Cedex (France)</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Plant Physiology</title><title level="j" type="abbrev">JPLPH</title><idno type="ISSN">0176-1617</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1984">1984</date><biblScope unit="volume">116</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="71">71</biblScope><biblScope unit="page" to="80">80</biblScope></imprint><idno type="ISSN">0176-1617</idno></series><idno type="istex">474F7761EBE3F5F9B9B5F00CED205AB1D98852D9</idno><idno type="DOI">10.1016/S0176-1617(84)80085-1</idno><idno type="PII">S0176-1617(84)80085-1</idno><idno type="ArticleID">80085</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0176-1617</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>3,4-dibydroxypbenylalanine accumulation</term><term>Vicia faba minor</term><term>stress physiology</term><term>symbiosis</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Summary: The uncommon amino acid 3,4-dihydroxyphenylalanine (DOPA) was determined colorimetrically using isonicotinic acid hydrazide (IAH) in the crude extracts obtained from fresh material of Vicia faba minor. DOPA was found to accumulate at concentrations inconsistent with its solubility in water. Other orthodiphenols do not interfere in DOPA determination. The accumulated DOPA was only of the L-form. The DOPA content in the dry material of Vicia faba was significantly lower than that from the corresponding fresh material, suggesting some kind of DOPA oxidation during drying. Subsequent analyses designed to evaluate the actual potential of V. faba minor in the accumulation of DOPA were performed with fresh material.It was observed that the DOPA concentration in the tissues of V. faba minor is reproducible when expressed on a fresh matter weight basis. In young seedlings L-DOPA production is significantly enhanced by light via an increased fresh matter yield. The DOPA content was found to be increased in mature plants grown on media enriched with low concentrations of NaCl (up to 25 mM) that showed a positive effect on their growth. NaCl induced an overproduction of L-DOPA even when growth was restricted by ion excess. On the contrary, the DOPA level dropped in mature plants submitted to water stress. Nodulated plants were found to contain higher content of L-DOPA than the non-nodulated ones grown with NO3- and NH4+. It is suggested that DOPA hyperproduction by Vicia faba minor might be optimized by growing plants in mild saline conditions and by inoculating them with efficient salt tolerant strains of R. leguminosarum.</div></front></TEI><istex><corpusName>elsevier</corpusName><author><json:item><name>F. Larher</name><affiliations><json:string>1) Laboratoire de Biologie végétale, U.E.R. Physique, Chimie, Biologie, Université de Nancy I, B.P. 239, F-54506 Vandoeuvre-les-Nancy Cedex (France)</json:string><json:string>To whom correspondence should be addressed.</json:string></affiliations></json:item><json:item><name>J. Gerard</name><affiliations><json:string>1) Laboratoire de Biologie végétale, U.E.R. Physique, Chimie, Biologie, Université de Nancy I, B.P. 239, F-54506 Vandoeuvre-les-Nancy Cedex (France)</json:string></affiliations></json:item><json:item><name>D. Gerant-Sauvage</name><affiliations><json:string>1) Laboratoire de Biologie végétale, U.E.R. Physique, Chimie, Biologie, Université de Nancy I, B.P. 239, F-54506 Vandoeuvre-les-Nancy Cedex (France)</json:string></affiliations></json:item><json:item><name>J. Hamelin</name><affiliations><json:string>Groupe de Recherche de Physicochimie structurale, U.E.R. Structure et Propriétés de la matière, Université de Rennes I, Campus de Beaulieu, F-35042 Rennes Cedex (France)</json:string></affiliations></json:item><json:item><name>M. Briens</name><affiliations><json:string>Laboratoire de Biologie végétale, U.E.R. Sciences de la vie et de l'environnement, Université de Rennes I, Campus de Beaulieu, F-35042 Rennes Cedex (France)</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>Vicia faba minor</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>3,4-dibydroxypbenylalanine accumulation</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>stress physiology</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>symbiosis</value></json:item></subject><articleId><json:string>80085</json:string></articleId><language><json:string>eng</json:string></language><originalGenre><json:string>Full-length article</json:string></originalGenre><abstract>Summary: The uncommon amino acid 3,4-dihydroxyphenylalanine (DOPA) was determined colorimetrically using isonicotinic acid hydrazide (IAH) in the crude extracts obtained from fresh material of Vicia faba minor. DOPA was found to accumulate at concentrations inconsistent with its solubility in water. Other orthodiphenols do not interfere in DOPA determination. The accumulated DOPA was only of the L-form. The DOPA content in the dry material of Vicia faba was significantly lower than that from the corresponding fresh material, suggesting some kind of DOPA oxidation during drying. Subsequent analyses designed to evaluate the actual potential of V. faba minor in the accumulation of DOPA were performed with fresh material.It was observed that the DOPA concentration in the tissues of V. faba minor is reproducible when expressed on a fresh matter weight basis. In young seedlings L-DOPA production is significantly enhanced by light via an increased fresh matter yield. The DOPA content was found to be increased in mature plants grown on media enriched with low concentrations of NaCl (up to 25 mM) that showed a positive effect on their growth. NaCl induced an overproduction of L-DOPA even when growth was restricted by ion excess. On the contrary, the DOPA level dropped in mature plants submitted to water stress. Nodulated plants were found to contain higher content of L-DOPA than the non-nodulated ones grown with NO3- and NH4+. It is suggested that DOPA hyperproduction by Vicia faba minor might be optimized by growing plants in mild saline conditions and by inoculating them with efficient salt tolerant strains of R. leguminosarum.</abstract><qualityIndicators><score>8.872</score><pdfVersion>1.7</pdfVersion><pdfPageSize>504 x 720 pts</pdfPageSize><refBibsNative>true</refBibsNative><keywordCount>4</keywordCount><abstractCharCount>1648</abstractCharCount><pdfWordCount>3872</pdfWordCount><pdfCharCount>21587</pdfCharCount><pdfPageCount>10</pdfPageCount><abstractWordCount>256</abstractWordCount></qualityIndicators><title>An Assessment of the Potential of Vicia faba minor in the Storage of the L-Form of 3,4-Dihydroxyphenylalanine</title><pii><json:string>S0176-1617(84)80085-1</json:string></pii><refBibs><json:item><author><json:item><name>R.L. Bieleski</name></json:item><json:item><name>N.A. 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Physique, Chimie, Biologie, Université de Nancy I, B.P. 239, F-54506 Vandoeuvre-les-Nancy Cedex (France)</affiliation><affiliation>To whom correspondence should be addressed.</affiliation></author><author xml:id="author-2"><persName><forename type="first">J.</forename><surname>Gerard</surname></persName><affiliation>1) Laboratoire de Biologie végétale, U.E.R. Physique, Chimie, Biologie, Université de Nancy I, B.P. 239, F-54506 Vandoeuvre-les-Nancy Cedex (France)</affiliation></author><author xml:id="author-3"><persName><forename type="first">D.</forename><surname>Gerant-Sauvage</surname></persName><affiliation>1) Laboratoire de Biologie végétale, U.E.R. Physique, Chimie, Biologie, Université de Nancy I, B.P. 239, F-54506 Vandoeuvre-les-Nancy Cedex (France)</affiliation></author><author xml:id="author-4"><persName><forename type="first">J.</forename><surname>Hamelin</surname></persName><affiliation>Groupe de Recherche de Physicochimie structurale, U.E.R. Structure et Propriétés de la matière, Université de Rennes I, Campus de Beaulieu, F-35042 Rennes Cedex (France)</affiliation></author><author xml:id="author-5"><persName><forename type="first">M.</forename><surname>Briens</surname></persName><affiliation>Laboratoire de Biologie végétale, U.E.R. Sciences de la vie et de l'environnement, Université de Rennes I, Campus de Beaulieu, F-35042 Rennes Cedex (France)</affiliation></author></analytic><monogr><title level="j">Journal of Plant Physiology</title><title level="j" type="abbrev">JPLPH</title><idno type="pISSN">0176-1617</idno><idno type="PII">S0176-1617(84)X8077-7</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1984"></date><biblScope unit="volume">116</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="71">71</biblScope><biblScope unit="page" to="80">80</biblScope></imprint></monogr><idno type="istex">474F7761EBE3F5F9B9B5F00CED205AB1D98852D9</idno><idno type="DOI">10.1016/S0176-1617(84)80085-1</idno><idno type="PII">S0176-1617(84)80085-1</idno><idno type="ArticleID">80085</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1984</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Summary: The uncommon amino acid 3,4-dihydroxyphenylalanine (DOPA) was determined colorimetrically using isonicotinic acid hydrazide (IAH) in the crude extracts obtained from fresh material of Vicia faba minor. DOPA was found to accumulate at concentrations inconsistent with its solubility in water. Other orthodiphenols do not interfere in DOPA determination. The accumulated DOPA was only of the L-form. The DOPA content in the dry material of Vicia faba was significantly lower than that from the corresponding fresh material, suggesting some kind of DOPA oxidation during drying. Subsequent analyses designed to evaluate the actual potential of V. faba minor in the accumulation of DOPA were performed with fresh material.It was observed that the DOPA concentration in the tissues of V. faba minor is reproducible when expressed on a fresh matter weight basis. In young seedlings L-DOPA production is significantly enhanced by light via an increased fresh matter yield. The DOPA content was found to be increased in mature plants grown on media enriched with low concentrations of NaCl (up to 25 mM) that showed a positive effect on their growth. NaCl induced an overproduction of L-DOPA even when growth was restricted by ion excess. On the contrary, the DOPA level dropped in mature plants submitted to water stress. Nodulated plants were found to contain higher content of L-DOPA than the non-nodulated ones grown with NO3- and NH4+. It is suggested that DOPA hyperproduction by Vicia faba minor might be optimized by growing plants in mild saline conditions and by inoculating them with efficient salt tolerant strains of R. leguminosarum.</p></abstract><textClass xml:lang="en"><keywords scheme="keyword"><list><head>Key words</head><item><term>Vicia faba minor</term></item><item><term>3,4-dibydroxypbenylalanine accumulation</term></item><item><term>stress physiology</term></item><item><term>symbiosis</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="1984">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/474F7761EBE3F5F9B9B5F00CED205AB1D98852D9/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier doc found" wicri:toSee="Elsevier, no converted or simple article"><istex:xmlDeclaration>version="1.0" encoding="UTF-8" </istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 5.1.0//EN//XML" URI="art510.dtd" name="istex:docType"></istex:docType><istex:document><article version="5.1" xml:lang="en" docsubtype="fla"><item-info><jid>JPLPH</jid><aid>80085</aid><ce:pii>S0176-1617(84)80085-1</ce:pii><ce:doi>10.1016/S0176-1617(84)80085-1</ce:doi><ce:copyright type="other" year="1984">Gustav Fischer Verlag, Stuttgart</ce:copyright></item-info><head><ce:title>An Assessment of the Potential of <ce:italic>Vicia faba</ce:italic> minor in the Storage of the L-Form of 3,4-Dihydroxyphenylalanine</ce:title><ce:author-group><ce:author><ce:given-name>F.</ce:given-name><ce:surname>Larher</ce:surname><ce:cross-ref refid="aff1"><ce:sup>1)</ce:sup></ce:cross-ref><ce:cross-ref refid="cor1"><ce:sup>*)</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>J.</ce:given-name><ce:surname>Gerard</ce:surname><ce:cross-ref refid="aff1"><ce:sup>1)</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>D.</ce:given-name><ce:surname>Gerant-Sauvage</ce:surname><ce:cross-ref refid="aff1"><ce:sup>1)</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>J.</ce:given-name><ce:surname>Hamelin</ce:surname><ce:cross-ref refid="aff2"><ce:sup>2)</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>M.</ce:given-name><ce:surname>Briens</ce:surname><ce:cross-ref refid="aff3"><ce:sup>3)</ce:sup></ce:cross-ref></ce:author><ce:affiliation id="aff1"><ce:textfn>1) Laboratoire de Biologie végétale, U.E.R. Physique, Chimie, Biologie, Université de Nancy I, B.P. 239, F-54506 Vandoeuvre-les-Nancy Cedex (France)</ce:textfn></ce:affiliation><ce:affiliation id="aff2"><ce:label>2)</ce:label><ce:textfn>Groupe de Recherche de Physicochimie structurale, U.E.R. Structure et Propriétés de la matière, Université de Rennes I, Campus de Beaulieu, F-35042 Rennes Cedex (France)</ce:textfn></ce:affiliation><ce:affiliation id="aff3"><ce:label>3)</ce:label><ce:textfn>Laboratoire de Biologie végétale, U.E.R. Sciences de la vie et de l'environnement, Université de Rennes I, Campus de Beaulieu, F-35042 Rennes Cedex (France)</ce:textfn></ce:affiliation><ce:correspondence id="cor1"><ce:label>*)</ce:label><ce:text>To whom correspondence should be addressed.</ce:text></ce:correspondence></ce:author-group><ce:date-received day="21" month="2" year="1984"></ce:date-received><ce:date-accepted day="16" month="4" year="1984"></ce:date-accepted><ce:abstract id="ab1" class="author" xml:lang="en"><ce:section-title>Summary</ce:section-title><ce:abstract-sec><ce:simple-para id="SP0005">The uncommon amino acid 3,4-dihydroxyphenylalanine (DOPA) was determined colorimetrically using isonicotinic acid hydrazide (IAH) in the crude extracts obtained from fresh material of <ce:italic>Vicia faba minor</ce:italic>. DOPA was found to accumulate at concentrations inconsistent with its solubility in water. Other orthodiphenols do not interfere in DOPA determination. The accumulated DOPA was only of the L-form. The DOPA content in the dry material of <ce:italic>Vicia faba</ce:italic> was significantly lower than that from the corresponding fresh material, suggesting some kind of DOPA oxidation during drying. Subsequent analyses designed to evaluate the actual potential of <ce:italic>V. faba</ce:italic> minor in the accumulation of DOPA were performed with fresh material.</ce:simple-para><ce:simple-para id="SP0010">It was observed that the DOPA concentration in the tissues of <ce:italic>V. faba minor</ce:italic> is reproducible when expressed on a fresh matter weight basis. In young seedlings L-DOPA production is significantly enhanced by light <ce:italic>via</ce:italic> an increased fresh matter yield. The DOPA content was found to be increased in mature plants grown on media enriched with low concentrations of NaCl (up to 25 mM) that showed a positive effect on their growth. NaCl induced an overproduction of L-DOPA even when growth was restricted by ion excess. On the contrary, the DOPA level dropped in mature plants submitted to water stress. Nodulated plants were found to contain higher content of L-DOPA than the non-nodulated ones grown with NO<ce:inf loc="post">3</ce:inf><ce:sup loc="post">-</ce:sup> and NH<ce:inf loc="post">4</ce:inf><ce:sup loc="post">+</ce:sup>. It is suggested that DOPA hyperproduction by <ce:italic>Vicia faba minor</ce:italic> might be optimized by growing plants in mild saline conditions and by inoculating them with efficient salt tolerant strains of <ce:italic>R. leguminosarum</ce:italic>.</ce:simple-para></ce:abstract-sec></ce:abstract><ce:keywords class="keyword" xml:lang="en"><ce:section-title>Key words</ce:section-title><ce:keyword><ce:text>Vicia faba minor</ce:text></ce:keyword><ce:keyword><ce:text>3,4-dibydroxypbenylalanine accumulation</ce:text></ce:keyword><ce:keyword><ce:text>stress physiology</ce:text></ce:keyword><ce:keyword><ce:text>symbiosis</ce:text></ce:keyword></ce:keywords></head><tail><ce:bibliography id="R0005"><ce:section-title>References</ce:section-title><ce:bibliography-sec id="RS0005"><ce:bib-reference id="bib1"><ce:label>BIELESKI and TURNER, 1966</ce:label><sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Bieleski</ce:surname><ce:given-name>R.L.</ce:given-name></sb:author><sb:author><ce:given-name>N.A.</ce:given-name><ce:surname>Turner</ce:surname></sb:author></sb:authors><sb:title><sb:maintitle>Separation and estimation of Amino acids in crude plant extracts by thin layer electrophoresis and chromatography</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>Analyt. 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Physique, Chimie, Biologie, Université de Nancy I, B.P. 239, F-54506 Vandoeuvre-les-Nancy Cedex (France)</affiliation><affiliation>To whom correspondence should be addressed.</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J.</namePart><namePart type="family">Gerard</namePart><affiliation>1) Laboratoire de Biologie végétale, U.E.R. Physique, Chimie, Biologie, Université de Nancy I, B.P. 239, F-54506 Vandoeuvre-les-Nancy Cedex (France)</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">D.</namePart><namePart type="family">Gerant-Sauvage</namePart><affiliation>1) Laboratoire de Biologie végétale, U.E.R. Physique, Chimie, Biologie, Université de Nancy I, B.P. 239, F-54506 Vandoeuvre-les-Nancy Cedex (France)</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J.</namePart><namePart type="family">Hamelin</namePart><affiliation>Groupe de Recherche de Physicochimie structurale, U.E.R. Structure et Propriétés de la matière, Université de Rennes I, Campus de Beaulieu, F-35042 Rennes Cedex (France)</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M.</namePart><namePart type="family">Briens</namePart><affiliation>Laboratoire de Biologie végétale, U.E.R. Sciences de la vie et de l'environnement, Université de Rennes I, Campus de Beaulieu, F-35042 Rennes Cedex (France)</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article"></genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1984</dateIssued><copyrightDate encoding="w3cdtf">1984</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType></physicalDescription><abstract lang="en">Summary: The uncommon amino acid 3,4-dihydroxyphenylalanine (DOPA) was determined colorimetrically using isonicotinic acid hydrazide (IAH) in the crude extracts obtained from fresh material of Vicia faba minor. DOPA was found to accumulate at concentrations inconsistent with its solubility in water. Other orthodiphenols do not interfere in DOPA determination. The accumulated DOPA was only of the L-form. The DOPA content in the dry material of Vicia faba was significantly lower than that from the corresponding fresh material, suggesting some kind of DOPA oxidation during drying. Subsequent analyses designed to evaluate the actual potential of V. faba minor in the accumulation of DOPA were performed with fresh material.It was observed that the DOPA concentration in the tissues of V. faba minor is reproducible when expressed on a fresh matter weight basis. In young seedlings L-DOPA production is significantly enhanced by light via an increased fresh matter yield. The DOPA content was found to be increased in mature plants grown on media enriched with low concentrations of NaCl (up to 25 mM) that showed a positive effect on their growth. NaCl induced an overproduction of L-DOPA even when growth was restricted by ion excess. On the contrary, the DOPA level dropped in mature plants submitted to water stress. Nodulated plants were found to contain higher content of L-DOPA than the non-nodulated ones grown with NO3- and NH4+. It is suggested that DOPA hyperproduction by Vicia faba minor might be optimized by growing plants in mild saline conditions and by inoculating them with efficient salt tolerant strains of R. leguminosarum.</abstract><subject lang="en"><genre>Key words</genre><topic>Vicia faba minor</topic><topic>3,4-dibydroxypbenylalanine accumulation</topic><topic>stress physiology</topic><topic>symbiosis</topic></subject><relatedItem type="host"><titleInfo><title>Journal of Plant Physiology</title></titleInfo><titleInfo type="abbreviated"><title>JPLPH</title></titleInfo><genre type="journal">journal</genre><originInfo><dateIssued encoding="w3cdtf">198408</dateIssued></originInfo><identifier type="ISSN">0176-1617</identifier><identifier type="PII">S0176-1617(84)X8077-7</identifier><part><date>198408</date><detail type="volume"><number>116</number><caption>vol.</caption></detail><detail type="issue"><number>1</number><caption>no.</caption></detail><extent unit="issue pages"><start>1</start><end>94</end></extent><extent unit="pages"><start>71</start><end>80</end></extent></part></relatedItem><identifier type="istex">474F7761EBE3F5F9B9B5F00CED205AB1D98852D9</identifier><identifier type="DOI">10.1016/S0176-1617(84)80085-1</identifier><identifier type="PII">S0176-1617(84)80085-1</identifier><identifier type="ArticleID">80085</identifier><accessCondition type="use and reproduction" contentType="copyright">©1984 Gustav Fischer Verlag, Stuttgart</accessCondition><recordInfo><recordContentSource>ELSEVIER</recordContentSource><recordOrigin>Gustav Fischer Verlag, Stuttgart, ©1984</recordOrigin></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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