Effects of the freezing rate on viability and fertility of frozen-thawed fowl spermatozoa
Identifieur interne : 000807 ( Istex/Corpus ); précédent : 000806; suivant : 000808Effects of the freezing rate on viability and fertility of frozen-thawed fowl spermatozoa
Auteurs : F. Seigneurin ; E. BlesboisSource :
- Theriogenology [ 0093-691X ] ; 1995.
Abstract
An improved method for the freezing and thawing of fowl semen was developed using glycerol as a cryoprotectant. The effect of the freezing rate was observed on the motility, viability plus morphology of spermatozoa and the fertility rate, as assessed on Days 2, 3 and 4 post insemination. Overall 5 to 6 twice-weekly successive inseminations were performed. In experiment 1, freezing rates of −1, −5, −10 and −15 °C/min from +5 to −35 °C, were tested for the above 4 parameters. The best results were obtained with freezing rates of −5 and −10 °C/min. In Experiment 2, the freezing rates of −5, −7 and −10 °C were tested for the fertilizing ability of the spermatozoa. The best freezing rate was −7 °C/min. The fertilizing ability of pooled frozen-thawed spermatozoa was very promising (76% fertilized oocytes). We conclude that even without the selection of parent stock animals (for fertilizing or laying ability) and without any selection of semen before freezing or after thawing, it is possible to obtain a high level of fertility and viability with this improved glycerol method following twice-weekly insemination with 300 million sperm cells.
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DOI: 10.1016/0093-691X(95)00119-S
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Seigneurin</name><affiliation><mods:affiliation>SYSAAF, Station de Recherches Avicoles, 37380 Nouzilly, France</mods:affiliation></affiliation></author><author><name sortKey="Blesbois, E" sort="Blesbois, E" uniqKey="Blesbois E" first="E." last="Blesbois">E. Blesbois</name><affiliation><mods:affiliation>INRA, Station de Recherches Avicoles, 37380 Nouzilly, France</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Theriogenology</title><title level="j" type="abbrev">THE</title><idno type="ISSN">0093-691X</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1995">1995</date><biblScope unit="volume">43</biblScope><biblScope unit="issue">8</biblScope><biblScope unit="page" from="1351">1351</biblScope><biblScope unit="page" to="1358">1358</biblScope></imprint><idno type="ISSN">0093-691X</idno></series><idno type="istex">F57B15BCBA625F48E0BDC2EE763B020C42DC6B70</idno><idno type="DOI">10.1016/0093-691X(95)00119-S</idno><idno type="PII">0093-691X(95)00119-S</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0093-691X</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">An improved method for the freezing and thawing of fowl semen was developed using glycerol as a cryoprotectant. The effect of the freezing rate was observed on the motility, viability plus morphology of spermatozoa and the fertility rate, as assessed on Days 2, 3 and 4 post insemination. Overall 5 to 6 twice-weekly successive inseminations were performed. In experiment 1, freezing rates of −1, −5, −10 and −15 °C/min from +5 to −35 °C, were tested for the above 4 parameters. The best results were obtained with freezing rates of −5 and −10 °C/min. In Experiment 2, the freezing rates of −5, −7 and −10 °C were tested for the fertilizing ability of the spermatozoa. The best freezing rate was −7 °C/min. The fertilizing ability of pooled frozen-thawed spermatozoa was very promising (76% fertilized oocytes). We conclude that even without the selection of parent stock animals (for fertilizing or laying ability) and without any selection of semen before freezing or after thawing, it is possible to obtain a high level of fertility and viability with this improved glycerol method following twice-weekly insemination with 300 million sperm cells.</div></front></TEI><istex><corpusName>elsevier</corpusName><author><json:item><name>F. Seigneurin</name><affiliations><json:string>SYSAAF, Station de Recherches Avicoles, 37380 Nouzilly, France</json:string></affiliations></json:item><json:item><name>E. 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The effect of the freezing rate was observed on the motility, viability plus morphology of spermatozoa and the fertility rate, as assessed on Days 2, 3 and 4 post insemination. Overall 5 to 6 twice-weekly successive inseminations were performed. In experiment 1, freezing rates of −1, −5, −10 and −15 °C/min from +5 to −35 °C, were tested for the above 4 parameters. The best results were obtained with freezing rates of −5 and −10 °C/min. In Experiment 2, the freezing rates of −5, −7 and −10 °C were tested for the fertilizing ability of the spermatozoa. The best freezing rate was −7 °C/min. The fertilizing ability of pooled frozen-thawed spermatozoa was very promising (76% fertilized oocytes). We conclude that even without the selection of parent stock animals (for fertilizing or laying ability) and without any selection of semen before freezing or after thawing, it is possible to obtain a high level of fertility and viability with this improved glycerol method following twice-weekly insemination with 300 million sperm cells.</abstract><qualityIndicators><score>4.71</score><pdfVersion>1.2</pdfVersion><pdfPageSize>468 x 648 pts</pdfPageSize><refBibsNative>true</refBibsNative><keywordCount>5</keywordCount><abstractCharCount>1150</abstractCharCount><pdfWordCount>2466</pdfWordCount><pdfCharCount>15496</pdfCharCount><pdfPageCount>8</pdfPageCount><abstractWordCount>187</abstractWordCount></qualityIndicators><title>Effects of the freezing rate on viability and fertility of frozen-thawed fowl spermatozoa</title><pii><json:string>0093-691X(95)00119-S</json:string></pii><refBibs><json:item><author><json:item><name>F Bellagamba</name></json:item><json:item><name>S Cerolini</name></json:item><json:item><name>L.G. 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Brockett</name></json:item></author><host><volume>30</volume><pages><last>431</last><first>423</first></pages><author></author><title>Cryobiology</title></host><title>Effects of sucrose, trehalose, hypotaurine, taurine and blood serum on survival of frozen bull sperm</title></json:item><json:item><author><json:item><name>P Dagnelie</name></json:item></author><host><pages><last>261</last><first>110</first></pages><author></author></host><title>Théorie et méthodes statistiques</title></json:item><json:item><author><json:item><name>U Haije</name></json:item></author><host><author></author><title>Ph. D. dissertation Rijksuniversiteit te Utrecht</title></host><title>Evaluation and Cryopreservation of Fowl-Semen (Gallus Domesticus)</title></json:item><json:item><author><json:item><name>P.E. Lake</name></json:item></author><host><author></author><title>VI Cong. Int Reprod Anim Insem Artif</title></host><serie><author></author><title>VI Cong. Int Reprod Anim Insem Artif</title></serie><title>Observations of freezing fowl spermatozoa in liquid nitrogen</title></json:item><json:item><author><json:item><name>P.E. Lake</name></json:item><json:item><name>J.M. Stewart</name></json:item></author><host><volume>19</volume><pages><last>194</last><first>187</first></pages><author></author><title>Br Poult Sci</title></host><title>Preservation of fowl semen in liquid nitrogen — an improved method</title></json:item><json:item><author><json:item><name>P.E Lake</name></json:item></author><host><author></author><title>Symp Zool Soc Lond</title></host><serie><author></author><title>Symp Zool Soc Lond</title></serie><title>The principals and practice of semen collection and preservation in birds</title></json:item><json:item><author><json:item><name>P.E. 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Whitfield</name></json:item></author><host><volume>27</volume><pages><last>796</last><first>781</first></pages><author></author><title>Theriogenology</title></host><title>Optimization of freezing conditions for bovine spermatozoa</title></json:item><json:item><author><json:item><name>T.J. Sexton</name></json:item></author><host><author></author><title>16th Cong. World's Poult Sci.</title></host><serie><author></author><title>16th Cong. World's Poult Sci.</title></serie><title>Viability of frozen chicken semen cooled at various rates to −20 °C in glass ampoules and plastic straws</title></json:item><json:item><author><json:item><name>T.J. Sexton</name></json:item></author><host><volume>59</volume><pages><last>2770</last><first>2765</first></pages><author></author><title>Poult Sci</title></host><title>Optimal rate for cooling chicken semen from +5 °C to −196 °C</title></json:item><json:item><author><json:item><name>G.J. Steyn</name></json:item></author><host><volume>30</volume><pages><last>590</last><first>581</first></pages><author></author><title>Cryobiology</title></host><title>The effect of freezing rate on the survival of cryopreserved African sharptooth catfish (Clarias gariepinus) spermatozoa</title></json:item><json:item><author><json:item><name>U Tajima</name></json:item><json:item><name>E.F. Graham</name></json:item><json:item><name>R.V. Shoffner</name></json:item><json:item><name>J.S. Otis</name></json:item><json:item><name>D.M. Hawkins</name></json:item></author><host><volume>69</volume><pages><last>1002</last><first>999</first></pages><author></author><title>Poult Sci</title></host><title>Cryopreservation of semen from unique lines of chicken germ plasm</title></json:item><json:item><author><json:item><name>G.J. Wishart</name></json:item></author><host><volume>26</volume><pages><last>380</last><first>375</first></pages><author></author><title>Br Poult Sci</title></host><title>Quantitation of the fertilizing ability of fresh compared with frozen and thawed fowl spermatozoa</title></json:item></refBibs><genre><json:string>research-article</json:string></genre><serie><volume>2</volume><pages><last>1635</last><first>1633</first></pages><language><json:string>unknown</json:string></language><title>VI Cong. 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The effect of the freezing rate was observed on the motility, viability plus morphology of spermatozoa and the fertility rate, as assessed on Days 2, 3 and 4 post insemination. Overall 5 to 6 twice-weekly successive inseminations were performed. In experiment 1, freezing rates of −1, −5, −10 and −15 °C/min from +5 to −35 °C, were tested for the above 4 parameters. The best results were obtained with freezing rates of −5 and −10 °C/min. In Experiment 2, the freezing rates of −5, −7 and −10 °C were tested for the fertilizing ability of the spermatozoa. The best freezing rate was −7 °C/min. The fertilizing ability of pooled frozen-thawed spermatozoa was very promising (76% fertilized oocytes). We conclude that even without the selection of parent stock animals (for fertilizing or laying ability) and without any selection of semen before freezing or after thawing, it is possible to obtain a high level of fertility and viability with this improved glycerol method following twice-weekly insemination with 300 million sperm cells.</p></abstract><textClass><keywords scheme="keyword"><list><head>Keywords</head><item><term>fowl semen</term></item><item><term>freezing rate</term></item><item><term>motility</term></item><item><term>viability</term></item><item><term>fertility</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="1995">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/F57B15BCBA625F48E0BDC2EE763B020C42DC6B70/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla"><item-info><jid>THE</jid><aid>9500119S</aid><ce:pii>0093-691X(95)00119-S</ce:pii><ce:doi>10.1016/0093-691X(95)00119-S</ce:doi><ce:copyright type="unknown" year="1995"></ce:copyright></item-info><head><ce:title>Effects of the freezing rate on viability and fertility of frozen-thawed fowl spermatozoa</ce:title><ce:author-group><ce:author><ce:given-name>F.</ce:given-name><ce:surname>Seigneurin</ce:surname><ce:cross-ref refid="AFF1"><ce:sup>1</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>E.</ce:given-name><ce:surname>Blesbois</ce:surname><ce:cross-ref refid="AFF2"><ce:sup>2</ce:sup></ce:cross-ref></ce:author><ce:affiliation id="AFF1"><ce:label>1</ce:label><ce:textfn>SYSAAF, Station de Recherches Avicoles, 37380 Nouzilly, France</ce:textfn></ce:affiliation><ce:affiliation id="AFF2"><ce:label>2</ce:label><ce:textfn>INRA, Station de Recherches Avicoles, 37380 Nouzilly, France</ce:textfn></ce:affiliation></ce:author-group><ce:date-received day="1" month="7" year="1994"></ce:date-received><ce:date-accepted day="8" month="11" year="1994"></ce:date-accepted><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>An improved method for the freezing and thawing of fowl semen was developed using glycerol as a cryoprotectant. The effect of the freezing rate was observed on the motility, viability plus morphology of spermatozoa and the fertility rate, as assessed on Days 2, 3 and 4 post insemination. Overall 5 to 6 twice-weekly successive inseminations were performed. In experiment 1, freezing rates of −1, −5, −10 and −15 °C/min from +5 to −35 °C, were tested for the above 4 parameters. The best results were obtained with freezing rates of −5 and −10 °C/min. In Experiment 2, the freezing rates of −5, −7 and −10 °C were tested for the fertilizing ability of the spermatozoa. The best freezing rate was −7 °C/min. The fertilizing ability of pooled frozen-thawed spermatozoa was very promising (76% fertilized oocytes).</ce:simple-para><ce:simple-para>We conclude that even without the selection of parent stock animals (for fertilizing or laying ability) and without any selection of semen before freezing or after thawing, it is possible to obtain a high level of fertility and viability with this improved glycerol method following twice-weekly insemination with 300 million sperm cells.</ce:simple-para></ce:abstract-sec></ce:abstract><ce:keywords><ce:section-title>Keywords</ce:section-title><ce:keyword><ce:text>fowl semen</ce:text></ce:keyword><ce:keyword><ce:text>freezing rate</ce:text></ce:keyword><ce:keyword><ce:text>motility</ce:text></ce:keyword><ce:keyword><ce:text>viability</ce:text></ce:keyword><ce:keyword><ce:text>fertility</ce:text></ce:keyword></ce:keywords></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><title>Effects of the freezing rate on viability and fertility of frozen-thawed fowl spermatozoa</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Effects of the freezing rate on viability and fertility of frozen-thawed fowl spermatozoa</title></titleInfo><name type="personal"><namePart type="given">F.</namePart><namePart type="family">Seigneurin</namePart><affiliation>SYSAAF, Station de Recherches Avicoles, 37380 Nouzilly, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">E.</namePart><namePart type="family">Blesbois</namePart><affiliation>INRA, Station de Recherches Avicoles, 37380 Nouzilly, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article"></genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1995</dateIssued><copyrightDate encoding="w3cdtf">1995</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType></physicalDescription><abstract lang="en">An improved method for the freezing and thawing of fowl semen was developed using glycerol as a cryoprotectant. The effect of the freezing rate was observed on the motility, viability plus morphology of spermatozoa and the fertility rate, as assessed on Days 2, 3 and 4 post insemination. Overall 5 to 6 twice-weekly successive inseminations were performed. In experiment 1, freezing rates of −1, −5, −10 and −15 °C/min from +5 to −35 °C, were tested for the above 4 parameters. The best results were obtained with freezing rates of −5 and −10 °C/min. In Experiment 2, the freezing rates of −5, −7 and −10 °C were tested for the fertilizing ability of the spermatozoa. The best freezing rate was −7 °C/min. The fertilizing ability of pooled frozen-thawed spermatozoa was very promising (76% fertilized oocytes). We conclude that even without the selection of parent stock animals (for fertilizing or laying ability) and without any selection of semen before freezing or after thawing, it is possible to obtain a high level of fertility and viability with this improved glycerol method following twice-weekly insemination with 300 million sperm cells.</abstract><subject><genre>Keywords</genre><topic>fowl semen</topic><topic>freezing rate</topic><topic>motility</topic><topic>viability</topic><topic>fertility</topic></subject><relatedItem type="host"><titleInfo><title>Theriogenology</title></titleInfo><titleInfo type="abbreviated"><title>THE</title></titleInfo><genre type="journal">journal</genre><originInfo><dateIssued encoding="w3cdtf">199506</dateIssued></originInfo><identifier type="ISSN">0093-691X</identifier><identifier type="PII">S0093-691X(00)X0015-5</identifier><part><date>199506</date><detail type="volume"><number>43</number><caption>vol.</caption></detail><detail type="issue"><number>8</number><caption>no.</caption></detail><extent unit="issue pages"><start>1289</start><end>1447</end></extent><extent unit="pages"><start>1351</start><end>1358</end></extent></part></relatedItem><identifier type="istex">F57B15BCBA625F48E0BDC2EE763B020C42DC6B70</identifier><identifier type="DOI">10.1016/0093-691X(95)00119-S</identifier><identifier type="PII">0093-691X(95)00119-S</identifier><recordInfo><recordContentSource>ELSEVIER</recordContentSource></recordInfo></mods></metadata></istex></record>Pour manipuler ce document sous Unix (Dilib)
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