High protection by grape seed proanthocyanidins (GSPC) of polyunsaturated fatty acids against UV-C induced peroxidation
Identifieur interne : 000555 ( Istex/Corpus ); précédent : 000554; suivant : 000556High protection by grape seed proanthocyanidins (GSPC) of polyunsaturated fatty acids against UV-C induced peroxidation
Auteurs : Rachid Bouhamidi ; Virginie Prévost ; André NouvelotSource :
- Comptes Rendus de l'Academie des Sciences Series III Sciences de la Vie [ 0764-4469 ] ; 1998.
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Abstract
Abstract: The antioxidative effects of grape seed proanthocyanidins (GSPC) were studied in three in-vitro models in which polyunsaturated fatty acids (PUFAs) in aqueous solution and mice liver or brain microsomes were used as oxidative substrates, and UVC irradiation as the pro-oxidant system. Analysis of UV-C induced lipid peroxidation was carried out by two methods: gas liquid chromatography of residual PUFAs and release of thiobarbituric acid-reactive substances (TBARs) measured by TBA reaction. Results indicate that PUFAs are more radiosensitive when incorporated in single component micelles than in mixed component micelles or microsomes. In every case, PUFA peroxidation was inhibited by low concentrations of GSPC (2 rng/L) while epigallocatecin (EGC) and epigallocatechin gallate (EGCG) monomers, at an equivalent level of epicatechin, exhibited no efficacy in our experimental conditions. This latter effect might be explained by a synergistic action of flavan-3-ol monomers, dimers and oligomers contained in the grape seed extract.
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DOI: 10.1016/S0764-4469(97)89623-0
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ISTEX:3792FB74D0E7B6FA8A00C137A0997A0C6F9618BCLe document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">High protection by grape seed proanthocyanidins (GSPC) of polyunsaturated fatty acids against UV-C induced peroxidation</title><author><name sortKey="Bouhamidi, Rachid" sort="Bouhamidi, Rachid" uniqKey="Bouhamidi R" first="Rachid" last="Bouhamidi">Rachid Bouhamidi</name><affiliation><mods:affiliation>Laboratoire de neurosciences, UMR 6551 du CNRS, université de Caen, bd H.-Becquerel, BP 5229, 14074 Caen cedex, France</mods:affiliation></affiliation></author><author><name sortKey="Prevost, Virginie" sort="Prevost, Virginie" uniqKey="Prevost V" first="Virginie" last="Prévost">Virginie Prévost</name><affiliation><mods:affiliation>Laboratoire de neurosciences, UMR 6551 du CNRS, université de Caen, bd H.-Becquerel, BP 5229, 14074 Caen cedex, France</mods:affiliation></affiliation></author><author><name sortKey="Nouvelot, Andre" sort="Nouvelot, Andre" uniqKey="Nouvelot A" first="André" last="Nouvelot">André Nouvelot</name><affiliation><mods:affiliation>Laboratoire de neurosciences, UMR 6551 du CNRS, université de Caen, bd H.-Becquerel, BP 5229, 14074 Caen cedex, France</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:3792FB74D0E7B6FA8A00C137A0997A0C6F9618BC</idno><date when="1998" year="1998">1998</date><idno type="doi">10.1016/S0764-4469(97)89623-0</idno><idno type="url">https://api.istex.fr/document/3792FB74D0E7B6FA8A00C137A0997A0C6F9618BC/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000555</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000555</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">High protection by grape seed proanthocyanidins (GSPC) of polyunsaturated fatty acids against UV-C induced peroxidation</title><author><name sortKey="Bouhamidi, Rachid" sort="Bouhamidi, Rachid" uniqKey="Bouhamidi R" first="Rachid" last="Bouhamidi">Rachid Bouhamidi</name><affiliation><mods:affiliation>Laboratoire de neurosciences, UMR 6551 du CNRS, université de Caen, bd H.-Becquerel, BP 5229, 14074 Caen cedex, France</mods:affiliation></affiliation></author><author><name sortKey="Prevost, Virginie" sort="Prevost, Virginie" uniqKey="Prevost V" first="Virginie" last="Prévost">Virginie Prévost</name><affiliation><mods:affiliation>Laboratoire de neurosciences, UMR 6551 du CNRS, université de Caen, bd H.-Becquerel, BP 5229, 14074 Caen cedex, France</mods:affiliation></affiliation></author><author><name sortKey="Nouvelot, Andre" sort="Nouvelot, Andre" uniqKey="Nouvelot A" first="André" last="Nouvelot">André Nouvelot</name><affiliation><mods:affiliation>Laboratoire de neurosciences, UMR 6551 du CNRS, université de Caen, bd H.-Becquerel, BP 5229, 14074 Caen cedex, France</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Comptes Rendus de l'Academie des Sciences Series III Sciences de la Vie</title><title level="j" type="abbrev">PXVOLD</title><idno type="ISSN">0764-4469</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1998">1998</date><biblScope unit="volume">321</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="31">31</biblScope><biblScope unit="page" to="38">38</biblScope></imprint><idno type="ISSN">0764-4469</idno></series><idno type="istex">3792FB74D0E7B6FA8A00C137A0997A0C6F9618BC</idno><idno type="DOI">10.1016/S0764-4469(97)89623-0</idno><idno type="PII">S0764-4469(97)89623-0</idno><idno type="ArticleID">97896230</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0764-4469</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>UV-C rays</term><term>grape seeds</term><term>lipoperoxidation</term><term>polyunsaturated fatty acids</term><term>proanthocyanidins</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: The antioxidative effects of grape seed proanthocyanidins (GSPC) were studied in three in-vitro models in which polyunsaturated fatty acids (PUFAs) in aqueous solution and mice liver or brain microsomes were used as oxidative substrates, and UVC irradiation as the pro-oxidant system. Analysis of UV-C induced lipid peroxidation was carried out by two methods: gas liquid chromatography of residual PUFAs and release of thiobarbituric acid-reactive substances (TBARs) measured by TBA reaction. Results indicate that PUFAs are more radiosensitive when incorporated in single component micelles than in mixed component micelles or microsomes. In every case, PUFA peroxidation was inhibited by low concentrations of GSPC (2 rng/L) while epigallocatecin (EGC) and epigallocatechin gallate (EGCG) monomers, at an equivalent level of epicatechin, exhibited no efficacy in our experimental conditions. This latter effect might be explained by a synergistic action of flavan-3-ol monomers, dimers and oligomers contained in the grape seed extract.</div></front></TEI><istex><corpusName>elsevier</corpusName><author><json:item><name>Rachid Bouhamidi</name><affiliations><json:string>Laboratoire de neurosciences, UMR 6551 du CNRS, université de Caen, bd H.-Becquerel, BP 5229, 14074 Caen cedex, France</json:string></affiliations></json:item><json:item><name>Virginie Prévost</name><affiliations><json:string>Laboratoire de neurosciences, UMR 6551 du CNRS, université de Caen, bd H.-Becquerel, BP 5229, 14074 Caen cedex, France</json:string></affiliations></json:item><json:item><name>André Nouvelot</name><affiliations><json:string>Laboratoire de neurosciences, UMR 6551 du CNRS, université de Caen, bd H.-Becquerel, BP 5229, 14074 Caen cedex, France</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>polyunsaturated fatty acids</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>UV-C rays</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>lipoperoxidation</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>proanthocyanidins</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>grape seeds</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>acides gras polyinsaturés</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>rayons UV-C</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>lipoperoxydation</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>proanthocyanidines</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>pépins de raisin</value></json:item></subject><articleId><json:string>97896230</json:string></articleId><language><json:string>eng</json:string></language><originalGenre><json:string>Full-length article</json:string></originalGenre><abstract>Abstract: The antioxidative effects of grape seed proanthocyanidins (GSPC) were studied in three in-vitro models in which polyunsaturated fatty acids (PUFAs) in aqueous solution and mice liver or brain microsomes were used as oxidative substrates, and UVC irradiation as the pro-oxidant system. Analysis of UV-C induced lipid peroxidation was carried out by two methods: gas liquid chromatography of residual PUFAs and release of thiobarbituric acid-reactive substances (TBARs) measured by TBA reaction. Results indicate that PUFAs are more radiosensitive when incorporated in single component micelles than in mixed component micelles or microsomes. In every case, PUFA peroxidation was inhibited by low concentrations of GSPC (2 rng/L) while epigallocatecin (EGC) and epigallocatechin gallate (EGCG) monomers, at an equivalent level of epicatechin, exhibited no efficacy in our experimental conditions. This latter effect might be explained by a synergistic action of flavan-3-ol monomers, dimers and oligomers contained in the grape seed extract.</abstract><qualityIndicators><score>3.828</score><pdfVersion>1.3</pdfVersion><pdfPageSize>612 x 792 pts (letter)</pdfPageSize><refBibsNative>true</refBibsNative><keywordCount>10</keywordCount><abstractCharCount>1049</abstractCharCount><pdfWordCount>2052</pdfWordCount><pdfCharCount>25016</pdfCharCount><pdfPageCount>8</pdfPageCount><abstractWordCount>148</abstractWordCount></qualityIndicators><title>High protection by grape seed proanthocyanidins (GSPC) of polyunsaturated fatty acids against UV-C induced peroxidation</title><pii><json:string>S0764-4469(97)89623-0</json:string></pii><refBibs><json:item><author><json:item><name>M. Fontecave</name></json:item><json:item><name>J.L. 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Radical-scavenging effects of tannins and related polyphenols on 1, 1-diphenyl-2-picrylhydrazyl radical</title></json:item></refBibs><genre><json:string>research-article</json:string></genre><serie><pages><last>40</last><first>21</first></pages><language><json:string>unknown</json:string></language><title>Free Radicals in the Brain: Aging, Neurological and Mental Disorders</title></serie><host><volume>321</volume><pii><json:string>S0764-4469(00)X0035-2</json:string></pii><pages><last>38</last><first>31</first></pages><issn><json:string>0764-4469</json:string></issn><issue>1</issue><genre><json:string>journal</json:string></genre><language><json:string>unknown</json:string></language><title>Comptes Rendus de l'Academie des Sciences Series III Sciences de la Vie</title><publicationDate>1998</publicationDate></host><categories><wos></wos><scienceMetrix><json:string>natural sciences</json:string><json:string>biology</json:string><json:string>evolutionary biology</json:string></scienceMetrix></categories><publicationDate>1998</publicationDate><copyrightDate>1998</copyrightDate><doi><json:string>10.1016/S0764-4469(97)89623-0</json:string></doi><id>3792FB74D0E7B6FA8A00C137A0997A0C6F9618BC</id><score>0.11800586</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/document/3792FB74D0E7B6FA8A00C137A0997A0C6F9618BC/fulltext/pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/document/3792FB74D0E7B6FA8A00C137A0997A0C6F9618BC/fulltext/zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/document/3792FB74D0E7B6FA8A00C137A0997A0C6F9618BC/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a" type="main" xml:lang="en">High protection by grape seed proanthocyanidins (GSPC) of polyunsaturated fatty acids against UV-C induced peroxidation</title><title level="a" type="alt" xml:lang="fr">Haute protection par les proanthocyanidines de pépins de raisin contre la peroxydation des acides gras polyinsaturés induite par les UV-C</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>ELSEVIER</publisher><availability><p>©1998 Académie des sciences / Elsevier</p></availability><date>1998</date></publicationStmt><notesStmt><note>*</note><note type="content">Section title: Plant biology and pathology</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a" type="main" xml:lang="en">High protection by grape seed proanthocyanidins (GSPC) of polyunsaturated fatty acids against UV-C induced peroxidation</title><title level="a" type="alt" xml:lang="fr">Haute protection par les proanthocyanidines de pépins de raisin contre la peroxydation des acides gras polyinsaturés induite par les UV-C</title><author xml:id="author-1"><persName><forename type="first">Rachid</forename><surname>Bouhamidi</surname></persName><affiliation>Laboratoire de neurosciences, UMR 6551 du CNRS, université de Caen, bd H.-Becquerel, BP 5229, 14074 Caen cedex, France</affiliation></author><author xml:id="author-2"><persName><forename type="first">Virginie</forename><surname>Prévost</surname></persName><affiliation>Laboratoire de neurosciences, UMR 6551 du CNRS, université de Caen, bd H.-Becquerel, BP 5229, 14074 Caen cedex, France</affiliation></author><author xml:id="author-3"><persName><forename type="first">André</forename><surname>Nouvelot</surname></persName><note type="correspondence"><p>Correspondence and reprints.</p></note><affiliation>Laboratoire de neurosciences, UMR 6551 du CNRS, université de Caen, bd H.-Becquerel, BP 5229, 14074 Caen cedex, France</affiliation></author></analytic><monogr><title level="j">Comptes Rendus de l'Academie des Sciences Series III Sciences de la Vie</title><title level="j" type="abbrev">PXVOLD</title><idno type="pISSN">0764-4469</idno><idno type="PII">S0764-4469(00)X0035-2</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1998"></date><biblScope unit="volume">321</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="31">31</biblScope><biblScope unit="page" to="38">38</biblScope></imprint></monogr><idno type="istex">3792FB74D0E7B6FA8A00C137A0997A0C6F9618BC</idno><idno type="DOI">10.1016/S0764-4469(97)89623-0</idno><idno type="PII">S0764-4469(97)89623-0</idno><idno type="ArticleID">97896230</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1998</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Abstract: The antioxidative effects of grape seed proanthocyanidins (GSPC) were studied in three in-vitro models in which polyunsaturated fatty acids (PUFAs) in aqueous solution and mice liver or brain microsomes were used as oxidative substrates, and UVC irradiation as the pro-oxidant system. Analysis of UV-C induced lipid peroxidation was carried out by two methods: gas liquid chromatography of residual PUFAs and release of thiobarbituric acid-reactive substances (TBARs) measured by TBA reaction. Results indicate that PUFAs are more radiosensitive when incorporated in single component micelles than in mixed component micelles or microsomes. In every case, PUFA peroxidation was inhibited by low concentrations of GSPC (2 rng/L) while epigallocatecin (EGC) and epigallocatechin gallate (EGCG) monomers, at an equivalent level of epicatechin, exhibited no efficacy in our experimental conditions. This latter effect might be explained by a synergistic action of flavan-3-ol monomers, dimers and oligomers contained in the grape seed extract.</p></abstract><abstract xml:lang="fr"><p>Résumé Resume L'effet antioxydant des proanthocyanidines de pépins de raisin a été étudié dans trois modèles in vitro. Des acides gras polyinsaturés (AGPI) en solution aqueuse ou des microsomes de foie et de cerveau de souris ont été utilisés comme substrats et l'irradiation UV-C comme système prooxydant. La peroxydation lipidique induite par les UV-C a été mesurée par deux méthodes: le dosage des AGPI résiduels par chromatographie en phase gazeuse et le dosage des substances réagissant avec lacide thiobarbiturique par la technique de 1 BA-réaction. Les résultats indiquent que les AGPI sont plus radiosensibles lorsqu'ils sont incorporés dans des micelles simples que lorsqu'ils sont incorporés dans des micelles contenant plusieurs AGPI ou des microsomes. Dans tous les cas, la peroxydation des AGPI est inhibée par de faibles concentrations de l'extrait proanthocyanidolique (2 mg/L) alors que l'épigallocatéchine et l'épigallocatéchine-gallate sous forme de monomères ne montrent aucune efficacité dans ces conditions expérimentales. Cela pourrait être expliqué par une action synergique des flavanes-3-ol monomères, dimères et trimères constituant l'extrait proanthocyanidolique de pépins de raisin.</p></abstract><textClass xml:lang="en"><keywords scheme="keyword"><list><item><term>polyunsaturated fatty acids</term></item><item><term>UV-C rays</term></item><item><term>lipoperoxidation</term></item><item><term>proanthocyanidins</term></item><item><term>grape seeds</term></item></list></keywords></textClass><textClass xml:lang="fr"><keywords scheme="keyword"><list><item><term>acides gras polyinsaturés</term></item><item><term>rayons UV-C</term></item><item><term>lipoperoxydation</term></item><item><term>proanthocyanidines</term></item><item><term>pépins de raisin</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="1998">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/3792FB74D0E7B6FA8A00C137A0997A0C6F9618BC/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier doc found" wicri:toSee="Elsevier, no converted or simple article"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 5.0.1//EN//XML" URI="art501.dtd" name="istex:docType"></istex:docType><istex:document><article version="5.0" xml:lang="en" docsubtype="fla"><item-info><jid>PXVOLD</jid><aid>97896230</aid><ce:pii>S0764-4469(97)89623-0</ce:pii><ce:doi>10.1016/S0764-4469(97)89623-0</ce:doi><ce:copyright type="unknown" year="1998">Académie des sciences / Elsevier</ce:copyright></item-info><head><ce:dochead><ce:textfn>Plant biology and pathology</ce:textfn></ce:dochead><ce:title>High protection by grape seed proanthocyanidins (GSPC) of polyunsaturated fatty acids against UV-C induced peroxidation</ce:title><ce:alt-title xml:lang="fr">Haute protection par les proanthocyanidines de pépins de raisin contre la peroxydation des acides gras polyinsaturés induite par les UV-C</ce:alt-title><ce:author-group><ce:author><ce:given-name>Rachid</ce:given-name><ce:surname>Bouhamidi</ce:surname></ce:author><ce:author><ce:given-name>Virginie</ce:given-name><ce:surname>Prévost</ce:surname></ce:author><ce:author><ce:given-name>André</ce:given-name><ce:surname>Nouvelot</ce:surname><ce:cross-ref refid="cor1"><ce:sup>*</ce:sup></ce:cross-ref></ce:author><ce:affiliation id="aff1"><ce:textfn>Laboratoire de neurosciences, UMR 6551 du CNRS, université de Caen, bd H.-Becquerel, BP 5229, 14074 Caen cedex, France</ce:textfn></ce:affiliation><ce:correspondence id="cor1"><ce:label>*</ce:label><ce:text>Correspondence and reprints.</ce:text></ce:correspondence></ce:author-group><ce:date-received day="13" month="8" year="1997"></ce:date-received><ce:date-accepted day="17" month="11" year="1997"></ce:date-accepted><ce:miscellaneous>*</ce:miscellaneous><ce:abstract id="ab1" class="author" xml:lang="en"><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>The antioxidative effects of grape seed proanthocyanidins (GSPC) were studied in three in-vitro models in which polyunsaturated fatty acids (PUFAs) in aqueous solution and mice liver or brain microsomes were used as oxidative substrates, and UVC irradiation as the pro-oxidant system. Analysis of UV-C induced lipid peroxidation was carried out by two methods: gas liquid chromatography of residual PUFAs and release of thiobarbituric acid-reactive substances (TBARs) measured by TBA reaction. Results indicate that PUFAs are more radiosensitive when incorporated in single component micelles than in mixed component micelles or microsomes. In every case, PUFA peroxidation was inhibited by low concentrations of GSPC (2 rng/L) while epigallocatecin (EGC) and epigallocatechin gallate (EGCG) monomers, at an equivalent level of epicatechin, exhibited no efficacy in our experimental conditions. This latter effect might be explained by a synergistic action of flavan-3-ol monomers, dimers and oligomers contained in the grape seed extract.</ce:simple-para></ce:abstract-sec></ce:abstract><ce:abstract id="ab2" class="author" xml:lang="fr"><ce:abstract-sec><ce:simple-para>Résumé Resume L'effet antioxydant des proanthocyanidines de pépins de raisin a été étudié dans trois modèles in vitro. Des acides gras polyinsaturés (AGPI) en solution aqueuse ou des microsomes de foie et de cerveau de souris ont été utilisés comme substrats et l'irradiation UV-C comme système prooxydant. La peroxydation lipidique induite par les UV-C a été mesurée par deux méthodes: le dosage des AGPI résiduels par chromatographie en phase gazeuse et le dosage des substances réagissant avec lacide thiobarbiturique par la technique de 1 BA-réaction. Les résultats indiquent que les AGPI sont plus radiosensibles lorsqu'ils sont incorporés dans des micelles simples que lorsqu'ils sont incorporés dans des micelles contenant plusieurs AGPI ou des microsomes. Dans tous les cas, la peroxydation des AGPI est inhibée par de faibles concentrations de l'extrait proanthocyanidolique (2 mg/L) alors que l'épigallocatéchine et l'épigallocatéchine-gallate sous forme de monomères ne montrent aucune efficacité dans ces conditions expérimentales. Cela pourrait être expliqué par une action synergique des flavanes-3-ol monomères, dimères et trimères constituant l'extrait proanthocyanidolique de pépins de raisin.</ce:simple-para></ce:abstract-sec></ce:abstract><ce:keywords class="keyword" xml:lang="en"><ce:keyword><ce:text>polyunsaturated fatty acids</ce:text></ce:keyword><ce:keyword><ce:text>UV-C rays</ce:text></ce:keyword><ce:keyword><ce:text>lipoperoxidation</ce:text></ce:keyword><ce:keyword><ce:text>proanthocyanidins</ce:text></ce:keyword><ce:keyword><ce:text>grape seeds</ce:text></ce:keyword></ce:keywords><ce:keywords class="keyword" xml:lang="fr"><ce:keyword><ce:text>acides gras polyinsaturés</ce:text></ce:keyword><ce:keyword><ce:text>rayons UV-C</ce:text></ce:keyword><ce:keyword><ce:text>lipoperoxydation</ce:text></ce:keyword><ce:keyword><ce:text>proanthocyanidines</ce:text></ce:keyword><ce:keyword><ce:text>pépins de raisin</ce:text></ce:keyword></ce:keywords></head><tail><ce:bibliography><ce:section-title>References</ce:section-title><ce:bibliography-sec><ce:bib-reference id="bib1"><ce:label>[1]</ce:label><sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Fontecave</ce:surname><ce:given-name>M.</ce:given-name></sb:author><sb:author><ce:surname>Pierre</ce:surname><ce:given-name>J.L.</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>Iron: Metabolism toxicity and therapy</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>Biochimie</sb:maintitle></sb:title><sb:volume-nr>75</sb:volume-nr></sb:series><sb:date>1995</sb:date></sb:issue><sb:pages><sb:first-page>767</sb:first-page><sb:last-page>773</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib2"><ce:label>[2]</ce:label><sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Ames</ce:surname><ce:given-name>B.N.</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>Endugenous oxidative DNA damage, aging and cancer</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>Free Rad. 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V. Radical-scavenging effects of tannins and related polyphenols on 1, 1-diphenyl-2-picrylhydrazyl radical</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>Chem. Pharm. Bull.</sb:maintitle></sb:title><sb:volume-nr>37</sb:volume-nr></sb:series><sb:date>1989</sb:date></sb:issue><sb:pages><sb:first-page>1919</sb:first-page><sb:last-page>1921</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference></ce:bibliography-sec></ce:bibliography></tail></article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>High protection by grape seed proanthocyanidins (GSPC) of polyunsaturated fatty acids against UV-C induced peroxidation</title></titleInfo><titleInfo type="alternative" lang="en" contentType="CDATA"><title>High protection by grape seed proanthocyanidins (GSPC) of polyunsaturated fatty acids against UV-C induced peroxidation</title></titleInfo><titleInfo type="translated" lang="fr"><title>Haute protection par les proanthocyanidines de pépins de raisin contre la peroxydation des acides gras polyinsaturés induite par les UV-C</title></titleInfo><name type="personal"><namePart type="given">Rachid</namePart><namePart type="family">Bouhamidi</namePart><affiliation>Laboratoire de neurosciences, UMR 6551 du CNRS, université de Caen, bd H.-Becquerel, BP 5229, 14074 Caen cedex, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Virginie</namePart><namePart type="family">Prévost</namePart><affiliation>Laboratoire de neurosciences, UMR 6551 du CNRS, université de Caen, bd H.-Becquerel, BP 5229, 14074 Caen cedex, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">André</namePart><namePart type="family">Nouvelot</namePart><affiliation>Laboratoire de neurosciences, UMR 6551 du CNRS, université de Caen, bd H.-Becquerel, BP 5229, 14074 Caen cedex, France</affiliation><description>Correspondence and reprints.</description><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article"></genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1998</dateIssued><copyrightDate encoding="w3cdtf">1998</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType></physicalDescription><abstract lang="en">Abstract: The antioxidative effects of grape seed proanthocyanidins (GSPC) were studied in three in-vitro models in which polyunsaturated fatty acids (PUFAs) in aqueous solution and mice liver or brain microsomes were used as oxidative substrates, and UVC irradiation as the pro-oxidant system. Analysis of UV-C induced lipid peroxidation was carried out by two methods: gas liquid chromatography of residual PUFAs and release of thiobarbituric acid-reactive substances (TBARs) measured by TBA reaction. Results indicate that PUFAs are more radiosensitive when incorporated in single component micelles than in mixed component micelles or microsomes. In every case, PUFA peroxidation was inhibited by low concentrations of GSPC (2 rng/L) while epigallocatecin (EGC) and epigallocatechin gallate (EGCG) monomers, at an equivalent level of epicatechin, exhibited no efficacy in our experimental conditions. This latter effect might be explained by a synergistic action of flavan-3-ol monomers, dimers and oligomers contained in the grape seed extract.</abstract><abstract lang="fr">Résumé Resume L'effet antioxydant des proanthocyanidines de pépins de raisin a été étudié dans trois modèles in vitro. Des acides gras polyinsaturés (AGPI) en solution aqueuse ou des microsomes de foie et de cerveau de souris ont été utilisés comme substrats et l'irradiation UV-C comme système prooxydant. La peroxydation lipidique induite par les UV-C a été mesurée par deux méthodes: le dosage des AGPI résiduels par chromatographie en phase gazeuse et le dosage des substances réagissant avec lacide thiobarbiturique par la technique de 1 BA-réaction. Les résultats indiquent que les AGPI sont plus radiosensibles lorsqu'ils sont incorporés dans des micelles simples que lorsqu'ils sont incorporés dans des micelles contenant plusieurs AGPI ou des microsomes. Dans tous les cas, la peroxydation des AGPI est inhibée par de faibles concentrations de l'extrait proanthocyanidolique (2 mg/L) alors que l'épigallocatéchine et l'épigallocatéchine-gallate sous forme de monomères ne montrent aucune efficacité dans ces conditions expérimentales. Cela pourrait être expliqué par une action synergique des flavanes-3-ol monomères, dimères et trimères constituant l'extrait proanthocyanidolique de pépins de raisin.</abstract><note>*</note><note type="content">Section title: Plant biology and pathology</note><subject lang="en"><topic>polyunsaturated fatty acids</topic><topic>UV-C rays</topic><topic>lipoperoxidation</topic><topic>proanthocyanidins</topic><topic>grape seeds</topic></subject><subject lang="fr"><topic>acides gras polyinsaturés</topic><topic>rayons UV-C</topic><topic>lipoperoxydation</topic><topic>proanthocyanidines</topic><topic>pépins de raisin</topic></subject><relatedItem type="host"><titleInfo><title>Comptes Rendus de l'Academie des Sciences Series III Sciences de la Vie</title></titleInfo><titleInfo type="abbreviated"><title>PXVOLD</title></titleInfo><genre type="journal">journal</genre><originInfo><dateIssued encoding="w3cdtf">199801</dateIssued></originInfo><identifier type="ISSN">0764-4469</identifier><identifier type="PII">S0764-4469(00)X0035-2</identifier><part><date>199801</date><detail type="volume"><number>321</number><caption>vol.</caption></detail><detail type="issue"><number>1</number><caption>no.</caption></detail><extent unit="issue pages"><start>1</start><end>85</end></extent><extent unit="pages"><start>31</start><end>38</end></extent></part></relatedItem><identifier type="istex">3792FB74D0E7B6FA8A00C137A0997A0C6F9618BC</identifier><identifier type="DOI">10.1016/S0764-4469(97)89623-0</identifier><identifier type="PII">S0764-4469(97)89623-0</identifier><identifier type="ArticleID">97896230</identifier><accessCondition type="use and reproduction" contentType="copyright">©1998 Académie des sciences / Elsevier</accessCondition><recordInfo><recordContentSource>ELSEVIER</recordContentSource><recordOrigin>Académie des sciences / Elsevier, ©1998</recordOrigin></recordInfo></mods></metadata></istex></record>Pour manipuler ce document sous Unix (Dilib)
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