A comparison between colonic submucosa and mucosa to detect Lewy pathology in Parkinson’s disease
Identifieur interne : 000405 ( Istex/Corpus ); précédent : 000404; suivant : 000406A comparison between colonic submucosa and mucosa to detect Lewy pathology in Parkinson’s disease
Auteurs : H. Pouclet ; T. Lebouvier ; E. Coron ; S. B. Des Varannes ; M. Neunlist ; P. DerkinderenSource :
- Neurogastroenterology & Motility [ 1350-1925 ] ; 2012-04.
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Abstract
Background Lewy bodies and neurites (LN), the two pathological hallmarks of Parkinson’s disease (PD), are found in the enteric nervous system (ENS). Previously, we have shown that whole mounts of submucosa obtained after microdissection of colonic biopsies can be used for the detection of LN in the submucosal plexus (SMP) of PD patients. Recent reports suggest that Lewy pathology may extend beyond the submucosa to involve the digestive mucosa. The aim of the present research was to determine whether the analysis of the mucosa obtained after microdissection may help improve the sensitivity of colonic biopsies to detect Lewy pathology in the colon of PD patients.
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DOI: 10.1111/j.1365-2982.2012.01887.x
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ISTEX:C82A33BD5A608F43313CFD809D984D6E0A50EDFELe document en format XML
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Previously, we have shown that whole mounts of submucosa obtained after microdissection of colonic biopsies can be used for the detection of LN in the submucosal plexus (SMP) of PD patients. Recent reports suggest that Lewy pathology may extend beyond the submucosa to involve the digestive mucosa. The aim of the present research was to determine whether the analysis of the mucosa obtained after microdissection may help improve the sensitivity of colonic biopsies to detect Lewy pathology in the colon of PD patients.</div></front></TEI><istex><corpusName>wiley</corpusName><author><json:item><name>H. Pouclet</name><affiliations><json:string>Inserm, U913, Nantes, France</json:string><json:string>Inserm, CIC‐04, Nantes, France</json:string><json:string>Nantes University, Nantes, France</json:string><json:string>CHU Nantes, Department of Neurology, Nantes, France</json:string></affiliations></json:item><json:item><name>T. 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level="a" type="main" xml:lang="en">A comparison between colonic submucosa and mucosa to detect Lewy pathology in Parkinson’s disease</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><availability><p>© 2012 Blackwell Publishing Ltd</p></availability><date>2012</date></publicationStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a" type="main" xml:lang="en">A comparison between colonic submucosa and mucosa to detect Lewy pathology in Parkinson’s disease</title><author xml:id="author-1"><persName><forename type="first">H.</forename><surname>Pouclet</surname></persName><affiliation>Inserm, U913, Nantes, France</affiliation><affiliation>Inserm, CIC‐04, Nantes, France</affiliation><affiliation>Nantes University, Nantes, France</affiliation><affiliation>CHU Nantes, Department of Neurology, Nantes, France</affiliation></author><author xml:id="author-2"><persName><forename type="first">T.</forename><surname>Lebouvier</surname></persName><affiliation>Inserm, U913, Nantes, France</affiliation><affiliation>Inserm, CIC‐04, Nantes, France</affiliation><affiliation>Nantes University, Nantes, France</affiliation><affiliation>CHU Nantes, Department of Neurology, Nantes, France</affiliation></author><author xml:id="author-3"><persName><forename type="first">E.</forename><surname>Coron</surname></persName><affiliation>Inserm, U913, Nantes, France</affiliation><affiliation>Inserm, CIC‐04, Nantes, France</affiliation><affiliation>Nantes University, Nantes, France</affiliation><affiliation>CHU Nantes, Institut des Maladies de l’Appareil Digestif, Nantes, France</affiliation></author><author xml:id="author-4"><persName><forename type="first">S. B.</forename><surname>Des Varannes</surname></persName><affiliation>Inserm, U913, Nantes, France</affiliation><affiliation>Inserm, CIC‐04, Nantes, France</affiliation><affiliation>Nantes University, Nantes, France</affiliation><affiliation>CHU Nantes, Institut des Maladies de l’Appareil Digestif, Nantes, France</affiliation></author><author xml:id="author-5"><persName><forename type="first">M.</forename><surname>Neunlist</surname></persName><affiliation>Inserm, U913, Nantes, France</affiliation><affiliation>Nantes University, Nantes, France</affiliation><affiliation>CHU Nantes, Institut des Maladies de l’Appareil Digestif, Nantes, France</affiliation></author><author xml:id="author-6"><persName><forename type="first">P.</forename><surname>Derkinderen</surname></persName><affiliation>Inserm, U913, Nantes, France</affiliation><affiliation>Inserm, CIC‐04, Nantes, France</affiliation><affiliation>Nantes University, Nantes, France</affiliation><affiliation>CHU Nantes, Department of Neurology, Nantes, France</affiliation><affiliation>CHU Nantes, Institut des Maladies de l’Appareil Digestif, Nantes, France</affiliation></author></analytic><monogr><title level="j">Neurogastroenterology & Motility</title><idno type="pISSN">1350-1925</idno><idno type="eISSN">1365-2982</idno><idno type="DOI">10.1111/(ISSN)1365-2982</idno><imprint><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="2012-04"></date><biblScope unit="volume">24</biblScope><biblScope unit="issue">4</biblScope><biblScope unit="page" from="202">e202</biblScope><biblScope unit="page" to="205">e205</biblScope></imprint></monogr><idno type="istex">C82A33BD5A608F43313CFD809D984D6E0A50EDFE</idno><idno type="DOI">10.1111/j.1365-2982.2012.01887.x</idno><idno type="ArticleID">NMO1887</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>2012</date></creation><langUsage><language ident="en">en</language></langUsage><abstract><p>Background Lewy bodies and neurites (LN), the two pathological hallmarks of Parkinson’s disease (PD), are found in the enteric nervous system (ENS). Previously, we have shown that whole mounts of submucosa obtained after microdissection of colonic biopsies can be used for the detection of LN in the submucosal plexus (SMP) of PD patients. Recent reports suggest that Lewy pathology may extend beyond the submucosa to involve the digestive mucosa. The aim of the present research was to determine whether the analysis of the mucosa obtained after microdissection may help improve the sensitivity of colonic biopsies to detect Lewy pathology in the colon of PD patients.</p></abstract><abstract><p>Methods Nine PD patients and 10 controls were included. Four biopsies were taken from the sigmoid/descending colon junction during the course of a rectosigmoidoscopy (short colonoscopy) in PD patients and during a total colonoscopy for colorectal screening in controls. Biopsies were microdissected, the mucosa was separated from the submucosa and both structures were analyzed by immunohistochemistry. Immunohistochemical analysis was performed using antibodies against phosphorylated alpha‐synuclein to detect LN and neurofilaments NF200 kDa to label the neuronal structures.</p></abstract><abstract><p>Key Results Lewy neurites were present in the SMP of four patients and in the mucosa of three patients. Remarkably, among the patients who displayed LN within their mucosa, one was devoid of Lewy pathology in his SMP. No LN were observed in the mucosa and the SMP of controls.</p></abstract><abstract><p>Conclusions & Inferences The parallel analysis of colonic mucosa, along with the SMP, can help detect Lewy pathology in PD.</p></abstract><textClass xml:lang="en"><keywords scheme="keyword"><list><head>keywords</head><item><term>biomarker</term></item><item><term>biopsies</term></item><item><term>colon</term></item><item><term>mucosa</term></item><item><term>Parkinson’s disease</term></item><item><term>submucosal plexus</term></item></list></keywords></textClass><textClass><keywords scheme="Journal Subject"><list><head>article-category</head><item><term>TECHNICAL NOTE</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="2012-04">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/C82A33BD5A608F43313CFD809D984D6E0A50EDFE/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Wiley, elements deleted: body"><istex:xmlDeclaration>version="1.0" encoding="UTF-8" standalone="yes"</istex:xmlDeclaration><istex:document><component version="2.0" type="serialArticle" xml:lang="en"><header><publicationMeta level="product"><publisherInfo><publisherName>Blackwell Publishing Ltd</publisherName><publisherLoc>Oxford, UK</publisherLoc></publisherInfo><doi origin="wiley" registered="yes">10.1111/(ISSN)1365-2982</doi><issn type="print">1350-1925</issn><issn type="electronic">1365-2982</issn><idGroup><id type="product" value="NMO"></id><id type="publisherDivision" value="ST"></id></idGroup><titleGroup><title type="main" sort="NEUROGASTROENTEROLOGY MOTILITY">Neurogastroenterology & Motility</title></titleGroup></publicationMeta><publicationMeta level="part" position="04104"><doi origin="wiley">10.1111/nmo.2012.24.issue-4</doi><numberingGroup><numbering type="journalVolume" number="24">24</numbering><numbering type="journalIssue" number="4">4</numbering></numberingGroup><coverDate startDate="2012-04">April 2012</coverDate></publicationMeta><publicationMeta level="unit" type="technicalNote" position="17" status="forIssue"><doi origin="wiley">10.1111/j.1365-2982.2012.01887.x</doi><idGroup><id type="unit" value="NMO1887"></id></idGroup><countGroup><count type="pageTotal" number="4"></count></countGroup><titleGroup><title type="tocHeading1">TECHNICAL NOTE</title><title type="articleCategory">TECHNICAL NOTE</title></titleGroup><copyright>© 2012 Blackwell Publishing Ltd</copyright><eventGroup><event type="xmlConverted" agent="Converter:BPG_TO_WML3G version:3.1.3 standalone mode:FullText" date="2012-03-13"></event><event type="publishedOnlineEarlyUnpaginated" date="2012-01-31"></event><event type="firstOnline" date="2012-01-31"></event><event type="publishedOnlineFinalForm" date="2012-03-13"></event><event type="xmlConverted" agent="Converter:WILEY_ML3G_TO_WILEY_ML3GV2 version:3.8.8" date="2014-02-03"></event><event type="xmlConverted" agent="Converter:WML3G_To_WML3G version:4.3.7 mode:FullText" date="2015-03-24"></event></eventGroup><numberingGroup><numbering type="pageFirst">e202</numbering><numbering type="pageLast">e205</numbering></numberingGroup><correspondenceTo><i>Address for Correspondence</i>
Pascal Derkinderen, Inserm U913, 1 place Alexis Ricordeau, 44093 Nantes, France.
Tel: 0033(0)240087924; fax: 0033(0)240087506;
e‐mail: <email>derkinderenp@yahoo.fr</email>; <email normalForm="pascal.derkinderen@chu-nantes.fr">pascal.derkinderen@chu‐nantes.fr</email></correspondenceTo><linkGroup><link type="toTypesetVersion" href="file:NMO.NMO1887.pdf"></link></linkGroup></publicationMeta><contentMeta><unparsedEditorialHistory><i>Received</i>: 25 October 2011 <i>Accepted for publication</i>: 11 January 2012</unparsedEditorialHistory><countGroup><count type="figureTotal" number="1"></count><count type="tableTotal" number="1"></count></countGroup><titleGroup><title type="main">A comparison between colonic submucosa and mucosa to detect Lewy pathology in Parkinson’s disease</title><title type="shortAuthors">H. Pouclet <i>et al.</i></title><title type="short">Colonic mucosa in Parkinson’s disease</title></titleGroup><creators><creator creatorRole="author" xml:id="cr1" affiliationRef="#a1 #a2 #a3 #a4"><personName><givenNames>H.</givenNames><familyName>Pouclet</familyName></personName></creator><creator creatorRole="author" xml:id="cr2" affiliationRef="#a1 #a2 #a3 #a4"><personName><givenNames>T.</givenNames><familyName>Lebouvier</familyName></personName></creator><creator creatorRole="author" xml:id="cr3" affiliationRef="#a1 #a2 #a3 #a5"><personName><givenNames>E.</givenNames><familyName>Coron</familyName></personName></creator><creator creatorRole="author" xml:id="cr4" affiliationRef="#a1 #a2 #a3 #a5"><personName><givenNames>S. B.</givenNames><familyName>Des Varannes</familyName></personName></creator><creator creatorRole="author" xml:id="cr5" affiliationRef="#a1 #a3 #a5"><personName><givenNames>M.</givenNames><familyName>Neunlist</familyName></personName></creator><creator creatorRole="author" xml:id="cr6" affiliationRef="#a1 #a2 #a3 #a4 #a5"><personName><givenNames>P.</givenNames><familyName>Derkinderen</familyName></personName></creator></creators><affiliationGroup><affiliation xml:id="a1" countryCode="FR"><unparsedAffiliation>Inserm, U913, Nantes, France</unparsedAffiliation></affiliation><affiliation xml:id="a2" countryCode="FR"><unparsedAffiliation>Inserm, CIC‐04, Nantes, France</unparsedAffiliation></affiliation><affiliation xml:id="a3" countryCode="FR"><unparsedAffiliation>Nantes University, Nantes, France</unparsedAffiliation></affiliation><affiliation xml:id="a4" countryCode="FR"><unparsedAffiliation>CHU Nantes, Department of Neurology, Nantes, France</unparsedAffiliation></affiliation><affiliation xml:id="a5" countryCode="FR"><unparsedAffiliation>CHU Nantes, Institut des Maladies de l’Appareil Digestif, Nantes, France</unparsedAffiliation></affiliation></affiliationGroup><keywordGroup xml:lang="en"><keyword xml:id="k1">biomarker</keyword><keyword xml:id="k2">biopsies</keyword><keyword xml:id="k3">colon</keyword><keyword xml:id="k4">mucosa</keyword><keyword xml:id="k5">Parkinson’s disease</keyword><keyword xml:id="k6">submucosal plexus</keyword></keywordGroup><abstractGroup><abstract type="main" xml:lang="en"><title type="main">Abstract</title><p><b>Background </b> Lewy bodies and neurites (LN), the two pathological hallmarks of Parkinson’s disease (PD), are found in the enteric nervous system (ENS). Previously, we have shown that whole mounts of submucosa obtained after microdissection of colonic biopsies can be used for the detection of LN in the submucosal plexus (SMP) of PD patients. Recent reports suggest that Lewy pathology may extend beyond the submucosa to involve the digestive mucosa. The aim of the present research was to determine whether the analysis of the mucosa obtained after microdissection may help improve the sensitivity of colonic biopsies to detect Lewy pathology in the colon of PD patients.</p><p><b>Methods </b> Nine PD patients and 10 controls were included. Four biopsies were taken from the sigmoid/descending colon junction during the course of a rectosigmoidoscopy (short colonoscopy) in PD patients and during a total colonoscopy for colorectal screening in controls. Biopsies were microdissected, the mucosa was separated from the submucosa and both structures were analyzed by immunohistochemistry. Immunohistochemical analysis was performed using antibodies against phosphorylated alpha‐synuclein to detect LN and neurofilaments NF200 kDa to label the neuronal structures.</p><p><b>Key Results </b> Lewy neurites were present in the SMP of four patients and in the mucosa of three patients. Remarkably, among the patients who displayed LN within their mucosa, one was devoid of Lewy pathology in his SMP. No LN were observed in the mucosa and the SMP of controls.</p><p><b>Conclusions & Inferences </b> The parallel analysis of colonic mucosa, along with the SMP, can help detect Lewy pathology in PD.</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>A comparison between colonic submucosa and mucosa to detect Lewy pathology in Parkinson’s disease</title></titleInfo><titleInfo type="abbreviated" lang="en"><title>Colonic mucosa in Parkinson’s disease</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>A comparison between colonic submucosa and mucosa to detect Lewy pathology in Parkinson’s disease</title></titleInfo><name type="personal"><namePart type="given">H.</namePart><namePart type="family">Pouclet</namePart><affiliation>Inserm, U913, Nantes, France</affiliation><affiliation>Inserm, CIC‐04, Nantes, France</affiliation><affiliation>Nantes University, Nantes, France</affiliation><affiliation>CHU Nantes, Department of Neurology, Nantes, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">T.</namePart><namePart type="family">Lebouvier</namePart><affiliation>Inserm, U913, Nantes, France</affiliation><affiliation>Inserm, CIC‐04, Nantes, France</affiliation><affiliation>Nantes University, Nantes, France</affiliation><affiliation>CHU Nantes, Department of Neurology, Nantes, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">E.</namePart><namePart type="family">Coron</namePart><affiliation>Inserm, U913, Nantes, France</affiliation><affiliation>Inserm, CIC‐04, Nantes, France</affiliation><affiliation>Nantes University, Nantes, France</affiliation><affiliation>CHU Nantes, Institut des Maladies de l’Appareil Digestif, Nantes, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">S. B.</namePart><namePart type="family">Des Varannes</namePart><affiliation>Inserm, U913, Nantes, France</affiliation><affiliation>Inserm, CIC‐04, Nantes, France</affiliation><affiliation>Nantes University, Nantes, France</affiliation><affiliation>CHU Nantes, Institut des Maladies de l’Appareil Digestif, Nantes, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M.</namePart><namePart type="family">Neunlist</namePart><affiliation>Inserm, U913, Nantes, France</affiliation><affiliation>Nantes University, Nantes, France</affiliation><affiliation>CHU Nantes, Institut des Maladies de l’Appareil Digestif, Nantes, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">P.</namePart><namePart type="family">Derkinderen</namePart><affiliation>Inserm, U913, Nantes, France</affiliation><affiliation>Inserm, CIC‐04, Nantes, France</affiliation><affiliation>Nantes University, Nantes, France</affiliation><affiliation>CHU Nantes, Department of Neurology, Nantes, France</affiliation><affiliation>CHU Nantes, Institut des Maladies de l’Appareil Digestif, Nantes, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="other" displayLabel="technicalNote"></genre><originInfo><publisher>Blackwell Publishing Ltd</publisher><place><placeTerm type="text">Oxford, UK</placeTerm></place><dateIssued encoding="w3cdtf">2012-04</dateIssued><edition>Received: 25 October 2011 Accepted for publication: 11 January 2012</edition><copyrightDate encoding="w3cdtf">2012</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType><extent unit="figures">1</extent><extent unit="tables">1</extent></physicalDescription><abstract>Background Lewy bodies and neurites (LN), the two pathological hallmarks of Parkinson’s disease (PD), are found in the enteric nervous system (ENS). Previously, we have shown that whole mounts of submucosa obtained after microdissection of colonic biopsies can be used for the detection of LN in the submucosal plexus (SMP) of PD patients. Recent reports suggest that Lewy pathology may extend beyond the submucosa to involve the digestive mucosa. The aim of the present research was to determine whether the analysis of the mucosa obtained after microdissection may help improve the sensitivity of colonic biopsies to detect Lewy pathology in the colon of PD patients.</abstract><abstract>Methods Nine PD patients and 10 controls were included. Four biopsies were taken from the sigmoid/descending colon junction during the course of a rectosigmoidoscopy (short colonoscopy) in PD patients and during a total colonoscopy for colorectal screening in controls. Biopsies were microdissected, the mucosa was separated from the submucosa and both structures were analyzed by immunohistochemistry. Immunohistochemical analysis was performed using antibodies against phosphorylated alpha‐synuclein to detect LN and neurofilaments NF200 kDa to label the neuronal structures.</abstract><abstract>Key Results Lewy neurites were present in the SMP of four patients and in the mucosa of three patients. Remarkably, among the patients who displayed LN within their mucosa, one was devoid of Lewy pathology in his SMP. No LN were observed in the mucosa and the SMP of controls.</abstract><abstract>Conclusions & Inferences The parallel analysis of colonic mucosa, along with the SMP, can help detect Lewy pathology in PD.</abstract><subject lang="en"><genre>keywords</genre><topic>biomarker</topic><topic>biopsies</topic><topic>colon</topic><topic>mucosa</topic><topic>Parkinson’s disease</topic><topic>submucosal plexus</topic></subject><relatedItem type="host"><titleInfo><title>Neurogastroenterology & Motility</title></titleInfo><genre type="journal">journal</genre><subject><genre>article-category</genre><topic>TECHNICAL NOTE</topic></subject><identifier type="ISSN">1350-1925</identifier><identifier type="eISSN">1365-2982</identifier><identifier type="DOI">10.1111/(ISSN)1365-2982</identifier><identifier type="PublisherID">NMO</identifier><part><date>2012</date><detail type="volume"><caption>vol.</caption><number>24</number></detail><detail type="issue"><caption>no.</caption><number>4</number></detail><extent unit="pages"><start>e202</start><end>e205</end><total>4</total></extent></part></relatedItem><identifier type="istex">C82A33BD5A608F43313CFD809D984D6E0A50EDFE</identifier><identifier type="DOI">10.1111/j.1365-2982.2012.01887.x</identifier><identifier type="ArticleID">NMO1887</identifier><accessCondition type="use and reproduction" contentType="copyright">© 2012 Blackwell Publishing Ltd</accessCondition><recordInfo><recordContentSource>WILEY</recordContentSource><recordOrigin>Blackwell Publishing Ltd</recordOrigin></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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