Cellular response of human neuroblastoma cells to α-synuclein fibrils, the main constituent of Lewy bodies.
Identifieur interne : 000109 ( Hal/Checkpoint ); précédent : 000108; suivant : 000110Cellular response of human neuroblastoma cells to α-synuclein fibrils, the main constituent of Lewy bodies.
Auteurs : Laura Pieri [France] ; Philippe Chafey [France] ; Morgane Le Gall [France] ; Guilhem Clary [France] ; Ronald Melki [France] ; Virginie Redeker [France]Source :
- BBA - Biochimica et Biophysica Acta [ 0006-3002 ] ; 2015-12-31.
Abstract
BACKGROUND:α-Synuclein (α-Syn) fibrils are the main constituent of Lewy bodies and a neuropathological hallmark of Parkinson's disease (PD). The propagation of α-Syn assemblies from cell to cell suggests that they are involved in PD progression. We previously showed that α-Syn fibrils are toxic because of their ability to bind and permeabilize cell membranes. Here, we document the cellular response in terms of proteome changes of SH-SY5Y cells exposed to exogenous α-Syn fibrils.METHODS:We compare the proteomes of cells of neuronal origin exposed or not either to oligomeric or fibrillar α-Syn using two dimensional differential in-gel electrophoresis (2D-DIGE) and mass spectrometry.RESULTS:Only α-Syn fibrils induce significant changes in the proteome of SH-SY5Y cells. In addition to proteins associated to apoptosis and toxicity, or proteins previously linked to neurodegenerative diseases, we report an overexpression of proteins involved in intracellular vesicle trafficking. We also report a remarkable increase in fibrillar α-Syn heterogeneity, mainly due to C-terminal truncations.CONCLUSIONS:Our results show that cells of neuronal origin adapt their proteome to exogenous α-Syn fibrils and actively modify those assemblies.GENERAL SIGNIFICANCE:Cells of neuronal origin adapt their proteome to exogenous toxic α-Syn fibrils and actively modify those assemblies. Our results bring insights into the cellular response and clearance events the cells implement to face the propagation of α-Syn assemblies associated to pathology.
Url:
DOI: 10.1016/j.bbagen.2015.10.007
Links toward previous steps (curation, corpus...)
Links to Exploration step
Hal:hal-01240193Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Cellular response of human neuroblastoma cells to α-synuclein fibrils, the main constituent of Lewy bodies.</title>
<author><name sortKey="Pieri, Laura" sort="Pieri, Laura" uniqKey="Pieri L" first="Laura" last="Pieri">Laura Pieri</name>
<affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-418266" status="VALID"> <orgName>Institut des Neurosciences de Paris-Saclay</orgName>
<orgName type="acronym">Neuro-PSI</orgName>
<desc> <address> <addrLine>Centre National de la Recherche Scientifique, Unité Mixte de Recherche-9197 Université Paris-Sudbâtiment 32 Campus de Recherche de Gif-sur-Yvette1, avenue de la Terrasse91198 Gif-sur-Yvette</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://neuro-psi.fr</ref>
</desc>
<listRelation> <relation active="#struct-92966" type="direct"></relation>
<relation name="UMR9197" active="#struct-441569" type="direct"></relation>
</listRelation>
<tutelles><tutelle active="#struct-92966" type="direct"><org type="institution" xml:id="struct-92966" status="VALID"> <orgName>Université Paris-Sud - Paris 11</orgName>
<orgName type="acronym">UP11</orgName>
<desc> <address> <addrLine>Bâtiment 300 - 91405 Orsay cedex</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.u-psud.fr/</ref>
</desc>
</org>
</tutelle>
<tutelle name="UMR9197" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID"> <idno type="IdRef">02636817X</idno>
<idno type="ISNI">0000000122597504</idno>
<orgName>Centre National de la Recherche Scientifique</orgName>
<orgName type="acronym">CNRS</orgName>
<date type="start">1939-10-19</date>
<desc> <address> <country key="FR"></country>
</address>
<ref type="url">http://www.cnrs.fr/</ref>
</desc>
</org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>France</country>
</affiliation>
</author>
<author><name sortKey="Chafey, Philippe" sort="Chafey, Philippe" uniqKey="Chafey P" first="Philippe" last="Chafey">Philippe Chafey</name>
<affiliation wicri:level="1"><hal:affiliation type="researchteam" xml:id="struct-401129" status="INCOMING"> <orgName>Institut Cochin, INSERM U1016, CNRS UMR 8104, Université Paris Descartes</orgName>
<desc> <address> <country key="FR"></country>
</address>
</desc>
<listRelation> <relation active="#struct-106187" type="direct"></relation>
<relation active="#struct-303171" type="indirect"></relation>
<relation active="#struct-301664" type="indirect"></relation>
<relation name="UMR 8104" active="#struct-441569" type="indirect"></relation>
<relation name="U1016" active="#struct-303623" type="indirect"></relation>
<relation active="#struct-189806" type="direct"></relation>
<relation active="#struct-300068" type="indirect"></relation>
<relation active="#struct-300123" type="indirect"></relation>
</listRelation>
<tutelles><tutelle active="#struct-106187" type="direct"><org type="laboratory" xml:id="struct-106187" status="VALID"> <idno type="IdRef">158485130</idno>
<idno type="RNSR">200217519N</idno>
<orgName>Institut Cochin</orgName>
<date type="start">2002</date>
<desc> <address> <addrLine>22 rue Méchain, 75014 Paris</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://cochin.inserm.fr/</ref>
</desc>
<listRelation> <relation active="#struct-303171" type="direct"></relation>
<relation active="#struct-301664" type="direct"></relation>
<relation name="UMR 8104" active="#struct-441569" type="direct"></relation>
<relation name="U1016" active="#struct-303623" type="direct"></relation>
</listRelation>
</org>
</tutelle>
<tutelle active="#struct-303171" type="indirect"><org type="institution" xml:id="struct-303171" status="VALID"> <orgName>Université Sorbonne Paris Cité</orgName>
<orgName type="acronym">USPC</orgName>
<desc> <address> <country key="FR"></country>
</address>
<ref type="url">http://www.sorbonne-paris-cite.fr/fr</ref>
</desc>
</org>
</tutelle>
<tutelle active="#struct-301664" type="indirect"><org type="institution" xml:id="struct-301664" status="VALID"><idno type="IdRef">026404788</idno>
<idno type="ISNI">0000 0001 2188 0914 </idno>
<orgName>Université Paris Descartes - Paris 5</orgName>
<orgName type="acronym">UPD5</orgName>
<desc><address><addrLine>12, rue de l'École de Médecine - 75270 Paris cedex 06</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.parisdescartes.fr/</ref>
</desc>
</org>
</tutelle>
<tutelle name="UMR 8104" active="#struct-441569" type="indirect"><org type="institution" xml:id="struct-441569" status="VALID"> <idno type="IdRef">02636817X</idno>
<idno type="ISNI">0000000122597504</idno>
<orgName>Centre National de la Recherche Scientifique</orgName>
<orgName type="acronym">CNRS</orgName>
<date type="start">1939-10-19</date>
<desc> <address> <country key="FR"></country>
</address>
<ref type="url">http://www.cnrs.fr/</ref>
</desc>
</org>
</tutelle>
<tutelle name="U1016" active="#struct-303623" type="indirect"><org type="institution" xml:id="struct-303623" status="VALID"> <idno type="IdRef">026388278</idno>
<orgName>Institut National de la Santé et de la Recherche Médicale</orgName>
<orgName type="acronym">INSERM</orgName>
<desc> <address> <addrLine>101, rue de Tolbiac, 75013 Paris </addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.inserm.fr</ref>
</desc>
</org>
</tutelle>
<tutelle active="#struct-189806" type="direct"><org type="laboratory" xml:id="struct-189806" status="VALID"> <orgName>Service de médecine interne et centre de référence des maladies rares [Cochin]</orgName>
<desc> <address> <addrLine>27, rue du Faubourg Saint-Jacques 75014 Paris</addrLine>
<country key="FR"></country>
</address>
</desc>
<listRelation> <relation active="#struct-300068" type="direct"></relation>
<relation active="#struct-300123" type="direct"></relation>
<relation active="#struct-301664" type="direct"></relation>
</listRelation>
</org>
</tutelle>
<tutelle active="#struct-300068" type="indirect"><org type="institution" xml:id="struct-300068" status="VALID"> <orgName>Assistance publique - Hôpitaux de Paris (AP-HP)</orgName>
<desc> <address> <country key="FR"></country>
</address>
</desc>
</org>
</tutelle>
<tutelle active="#struct-300123" type="indirect"><org type="institution" xml:id="struct-300123" status="VALID"> <orgName>CHU Cochin [APHP]</orgName>
<desc> <address> <addrLine>27 Rue du Faubourg Saint-Jacques 75014 Paris</addrLine>
<country key="FR"></country>
</address>
</desc>
</org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>France</country>
</affiliation>
</author>
<author><name sortKey="Le Gall, Morgane" sort="Le Gall, Morgane" uniqKey="Le Gall M" first="Morgane" last="Le Gall">Morgane Le Gall</name>
<affiliation wicri:level="1"><hal:affiliation type="researchteam" xml:id="struct-401129" status="INCOMING"> <orgName>Institut Cochin, INSERM U1016, CNRS UMR 8104, Université Paris Descartes</orgName>
<desc> <address> <country key="FR"></country>
</address>
</desc>
<listRelation> <relation active="#struct-106187" type="direct"></relation>
<relation active="#struct-303171" type="indirect"></relation>
<relation active="#struct-301664" type="indirect"></relation>
<relation name="UMR 8104" active="#struct-441569" type="indirect"></relation>
<relation name="U1016" active="#struct-303623" type="indirect"></relation>
<relation active="#struct-189806" type="direct"></relation>
<relation active="#struct-300068" type="indirect"></relation>
<relation active="#struct-300123" type="indirect"></relation>
</listRelation>
<tutelles><tutelle active="#struct-106187" type="direct"><org type="laboratory" xml:id="struct-106187" status="VALID"> <idno type="IdRef">158485130</idno>
<idno type="RNSR">200217519N</idno>
<orgName>Institut Cochin</orgName>
<date type="start">2002</date>
<desc> <address> <addrLine>22 rue Méchain, 75014 Paris</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://cochin.inserm.fr/</ref>
</desc>
<listRelation> <relation active="#struct-303171" type="direct"></relation>
<relation active="#struct-301664" type="direct"></relation>
<relation name="UMR 8104" active="#struct-441569" type="direct"></relation>
<relation name="U1016" active="#struct-303623" type="direct"></relation>
</listRelation>
</org>
</tutelle>
<tutelle active="#struct-303171" type="indirect"><org type="institution" xml:id="struct-303171" status="VALID"> <orgName>Université Sorbonne Paris Cité</orgName>
<orgName type="acronym">USPC</orgName>
<desc> <address> <country key="FR"></country>
</address>
<ref type="url">http://www.sorbonne-paris-cite.fr/fr</ref>
</desc>
</org>
</tutelle>
<tutelle active="#struct-301664" type="indirect"><org type="institution" xml:id="struct-301664" status="VALID"><idno type="IdRef">026404788</idno>
<idno type="ISNI">0000 0001 2188 0914 </idno>
<orgName>Université Paris Descartes - Paris 5</orgName>
<orgName type="acronym">UPD5</orgName>
<desc><address><addrLine>12, rue de l'École de Médecine - 75270 Paris cedex 06</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.parisdescartes.fr/</ref>
</desc>
</org>
</tutelle>
<tutelle name="UMR 8104" active="#struct-441569" type="indirect"><org type="institution" xml:id="struct-441569" status="VALID"> <idno type="IdRef">02636817X</idno>
<idno type="ISNI">0000000122597504</idno>
<orgName>Centre National de la Recherche Scientifique</orgName>
<orgName type="acronym">CNRS</orgName>
<date type="start">1939-10-19</date>
<desc> <address> <country key="FR"></country>
</address>
<ref type="url">http://www.cnrs.fr/</ref>
</desc>
</org>
</tutelle>
<tutelle name="U1016" active="#struct-303623" type="indirect"><org type="institution" xml:id="struct-303623" status="VALID"> <idno type="IdRef">026388278</idno>
<orgName>Institut National de la Santé et de la Recherche Médicale</orgName>
<orgName type="acronym">INSERM</orgName>
<desc> <address> <addrLine>101, rue de Tolbiac, 75013 Paris </addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.inserm.fr</ref>
</desc>
</org>
</tutelle>
<tutelle active="#struct-189806" type="direct"><org type="laboratory" xml:id="struct-189806" status="VALID"> <orgName>Service de médecine interne et centre de référence des maladies rares [Cochin]</orgName>
<desc> <address> <addrLine>27, rue du Faubourg Saint-Jacques 75014 Paris</addrLine>
<country key="FR"></country>
</address>
</desc>
<listRelation> <relation active="#struct-300068" type="direct"></relation>
<relation active="#struct-300123" type="direct"></relation>
<relation active="#struct-301664" type="direct"></relation>
</listRelation>
</org>
</tutelle>
<tutelle active="#struct-300068" type="indirect"><org type="institution" xml:id="struct-300068" status="VALID"> <orgName>Assistance publique - Hôpitaux de Paris (AP-HP)</orgName>
<desc> <address> <country key="FR"></country>
</address>
</desc>
</org>
</tutelle>
<tutelle active="#struct-300123" type="indirect"><org type="institution" xml:id="struct-300123" status="VALID"> <orgName>CHU Cochin [APHP]</orgName>
<desc> <address> <addrLine>27 Rue du Faubourg Saint-Jacques 75014 Paris</addrLine>
<country key="FR"></country>
</address>
</desc>
</org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>France</country>
</affiliation>
</author>
<author><name sortKey="Clary, Guilhem" sort="Clary, Guilhem" uniqKey="Clary G" first="Guilhem" last="Clary">Guilhem Clary</name>
<affiliation wicri:level="1"><hal:affiliation type="researchteam" xml:id="struct-401129" status="INCOMING"> <orgName>Institut Cochin, INSERM U1016, CNRS UMR 8104, Université Paris Descartes</orgName>
<desc> <address> <country key="FR"></country>
</address>
</desc>
<listRelation> <relation active="#struct-106187" type="direct"></relation>
<relation active="#struct-303171" type="indirect"></relation>
<relation active="#struct-301664" type="indirect"></relation>
<relation name="UMR 8104" active="#struct-441569" type="indirect"></relation>
<relation name="U1016" active="#struct-303623" type="indirect"></relation>
<relation active="#struct-189806" type="direct"></relation>
<relation active="#struct-300068" type="indirect"></relation>
<relation active="#struct-300123" type="indirect"></relation>
</listRelation>
<tutelles><tutelle active="#struct-106187" type="direct"><org type="laboratory" xml:id="struct-106187" status="VALID"> <idno type="IdRef">158485130</idno>
<idno type="RNSR">200217519N</idno>
<orgName>Institut Cochin</orgName>
<date type="start">2002</date>
<desc> <address> <addrLine>22 rue Méchain, 75014 Paris</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://cochin.inserm.fr/</ref>
</desc>
<listRelation> <relation active="#struct-303171" type="direct"></relation>
<relation active="#struct-301664" type="direct"></relation>
<relation name="UMR 8104" active="#struct-441569" type="direct"></relation>
<relation name="U1016" active="#struct-303623" type="direct"></relation>
</listRelation>
</org>
</tutelle>
<tutelle active="#struct-303171" type="indirect"><org type="institution" xml:id="struct-303171" status="VALID"> <orgName>Université Sorbonne Paris Cité</orgName>
<orgName type="acronym">USPC</orgName>
<desc> <address> <country key="FR"></country>
</address>
<ref type="url">http://www.sorbonne-paris-cite.fr/fr</ref>
</desc>
</org>
</tutelle>
<tutelle active="#struct-301664" type="indirect"><org type="institution" xml:id="struct-301664" status="VALID"><idno type="IdRef">026404788</idno>
<idno type="ISNI">0000 0001 2188 0914 </idno>
<orgName>Université Paris Descartes - Paris 5</orgName>
<orgName type="acronym">UPD5</orgName>
<desc><address><addrLine>12, rue de l'École de Médecine - 75270 Paris cedex 06</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.parisdescartes.fr/</ref>
</desc>
</org>
</tutelle>
<tutelle name="UMR 8104" active="#struct-441569" type="indirect"><org type="institution" xml:id="struct-441569" status="VALID"> <idno type="IdRef">02636817X</idno>
<idno type="ISNI">0000000122597504</idno>
<orgName>Centre National de la Recherche Scientifique</orgName>
<orgName type="acronym">CNRS</orgName>
<date type="start">1939-10-19</date>
<desc> <address> <country key="FR"></country>
</address>
<ref type="url">http://www.cnrs.fr/</ref>
</desc>
</org>
</tutelle>
<tutelle name="U1016" active="#struct-303623" type="indirect"><org type="institution" xml:id="struct-303623" status="VALID"> <idno type="IdRef">026388278</idno>
<orgName>Institut National de la Santé et de la Recherche Médicale</orgName>
<orgName type="acronym">INSERM</orgName>
<desc> <address> <addrLine>101, rue de Tolbiac, 75013 Paris </addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.inserm.fr</ref>
</desc>
</org>
</tutelle>
<tutelle active="#struct-189806" type="direct"><org type="laboratory" xml:id="struct-189806" status="VALID"> <orgName>Service de médecine interne et centre de référence des maladies rares [Cochin]</orgName>
<desc> <address> <addrLine>27, rue du Faubourg Saint-Jacques 75014 Paris</addrLine>
<country key="FR"></country>
</address>
</desc>
<listRelation> <relation active="#struct-300068" type="direct"></relation>
<relation active="#struct-300123" type="direct"></relation>
<relation active="#struct-301664" type="direct"></relation>
</listRelation>
</org>
</tutelle>
<tutelle active="#struct-300068" type="indirect"><org type="institution" xml:id="struct-300068" status="VALID"> <orgName>Assistance publique - Hôpitaux de Paris (AP-HP)</orgName>
<desc> <address> <country key="FR"></country>
</address>
</desc>
</org>
</tutelle>
<tutelle active="#struct-300123" type="indirect"><org type="institution" xml:id="struct-300123" status="VALID"> <orgName>CHU Cochin [APHP]</orgName>
<desc> <address> <addrLine>27 Rue du Faubourg Saint-Jacques 75014 Paris</addrLine>
<country key="FR"></country>
</address>
</desc>
</org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>France</country>
</affiliation>
</author>
<author><name sortKey="Melki, Ronald" sort="Melki, Ronald" uniqKey="Melki R" first="Ronald" last="Melki">Ronald Melki</name>
<affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-418266" status="VALID"> <orgName>Institut des Neurosciences de Paris-Saclay</orgName>
<orgName type="acronym">Neuro-PSI</orgName>
<desc> <address> <addrLine>Centre National de la Recherche Scientifique, Unité Mixte de Recherche-9197 Université Paris-Sudbâtiment 32 Campus de Recherche de Gif-sur-Yvette1, avenue de la Terrasse91198 Gif-sur-Yvette</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://neuro-psi.fr</ref>
</desc>
<listRelation> <relation active="#struct-92966" type="direct"></relation>
<relation name="UMR9197" active="#struct-441569" type="direct"></relation>
</listRelation>
<tutelles><tutelle active="#struct-92966" type="direct"><org type="institution" xml:id="struct-92966" status="VALID"> <orgName>Université Paris-Sud - Paris 11</orgName>
<orgName type="acronym">UP11</orgName>
<desc> <address> <addrLine>Bâtiment 300 - 91405 Orsay cedex</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.u-psud.fr/</ref>
</desc>
</org>
</tutelle>
<tutelle name="UMR9197" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID"> <idno type="IdRef">02636817X</idno>
<idno type="ISNI">0000000122597504</idno>
<orgName>Centre National de la Recherche Scientifique</orgName>
<orgName type="acronym">CNRS</orgName>
<date type="start">1939-10-19</date>
<desc> <address> <country key="FR"></country>
</address>
<ref type="url">http://www.cnrs.fr/</ref>
</desc>
</org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>France</country>
</affiliation>
</author>
<author><name sortKey="Redeker, Virginie" sort="Redeker, Virginie" uniqKey="Redeker V" first="Virginie" last="Redeker">Virginie Redeker</name>
<affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-418266" status="VALID"> <orgName>Institut des Neurosciences de Paris-Saclay</orgName>
<orgName type="acronym">Neuro-PSI</orgName>
<desc> <address> <addrLine>Centre National de la Recherche Scientifique, Unité Mixte de Recherche-9197 Université Paris-Sudbâtiment 32 Campus de Recherche de Gif-sur-Yvette1, avenue de la Terrasse91198 Gif-sur-Yvette</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://neuro-psi.fr</ref>
</desc>
<listRelation> <relation active="#struct-92966" type="direct"></relation>
<relation name="UMR9197" active="#struct-441569" type="direct"></relation>
</listRelation>
<tutelles><tutelle active="#struct-92966" type="direct"><org type="institution" xml:id="struct-92966" status="VALID"> <orgName>Université Paris-Sud - Paris 11</orgName>
<orgName type="acronym">UP11</orgName>
<desc> <address> <addrLine>Bâtiment 300 - 91405 Orsay cedex</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.u-psud.fr/</ref>
</desc>
</org>
</tutelle>
<tutelle name="UMR9197" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID"> <idno type="IdRef">02636817X</idno>
<idno type="ISNI">0000000122597504</idno>
<orgName>Centre National de la Recherche Scientifique</orgName>
<orgName type="acronym">CNRS</orgName>
<date type="start">1939-10-19</date>
<desc> <address> <country key="FR"></country>
</address>
<ref type="url">http://www.cnrs.fr/</ref>
</desc>
</org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>France</country>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">HAL</idno>
<idno type="RBID">Hal:hal-01240193</idno>
<idno type="halId">hal-01240193</idno>
<idno type="halUri">https://hal.archives-ouvertes.fr/hal-01240193</idno>
<idno type="url">https://hal.archives-ouvertes.fr/hal-01240193</idno>
<idno type="doi">10.1016/j.bbagen.2015.10.007</idno>
<date when="2015-12-31">2015-12-31</date>
<idno type="wicri:Area/Hal/Corpus">000155</idno>
<idno type="wicri:Area/Hal/Curation">000155</idno>
<idno type="wicri:Area/Hal/Checkpoint">000109</idno>
<idno type="wicri:explorRef" wicri:stream="Hal" wicri:step="Checkpoint">000109</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Cellular response of human neuroblastoma cells to α-synuclein fibrils, the main constituent of Lewy bodies.</title>
<author><name sortKey="Pieri, Laura" sort="Pieri, Laura" uniqKey="Pieri L" first="Laura" last="Pieri">Laura Pieri</name>
<affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-418266" status="VALID"> <orgName>Institut des Neurosciences de Paris-Saclay</orgName>
<orgName type="acronym">Neuro-PSI</orgName>
<desc> <address> <addrLine>Centre National de la Recherche Scientifique, Unité Mixte de Recherche-9197 Université Paris-Sudbâtiment 32 Campus de Recherche de Gif-sur-Yvette1, avenue de la Terrasse91198 Gif-sur-Yvette</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://neuro-psi.fr</ref>
</desc>
<listRelation> <relation active="#struct-92966" type="direct"></relation>
<relation name="UMR9197" active="#struct-441569" type="direct"></relation>
</listRelation>
<tutelles><tutelle active="#struct-92966" type="direct"><org type="institution" xml:id="struct-92966" status="VALID"> <orgName>Université Paris-Sud - Paris 11</orgName>
<orgName type="acronym">UP11</orgName>
<desc> <address> <addrLine>Bâtiment 300 - 91405 Orsay cedex</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.u-psud.fr/</ref>
</desc>
</org>
</tutelle>
<tutelle name="UMR9197" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID"> <idno type="IdRef">02636817X</idno>
<idno type="ISNI">0000000122597504</idno>
<orgName>Centre National de la Recherche Scientifique</orgName>
<orgName type="acronym">CNRS</orgName>
<date type="start">1939-10-19</date>
<desc> <address> <country key="FR"></country>
</address>
<ref type="url">http://www.cnrs.fr/</ref>
</desc>
</org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>France</country>
</affiliation>
</author>
<author><name sortKey="Chafey, Philippe" sort="Chafey, Philippe" uniqKey="Chafey P" first="Philippe" last="Chafey">Philippe Chafey</name>
<affiliation wicri:level="1"><hal:affiliation type="researchteam" xml:id="struct-401129" status="INCOMING"> <orgName>Institut Cochin, INSERM U1016, CNRS UMR 8104, Université Paris Descartes</orgName>
<desc> <address> <country key="FR"></country>
</address>
</desc>
<listRelation> <relation active="#struct-106187" type="direct"></relation>
<relation active="#struct-303171" type="indirect"></relation>
<relation active="#struct-301664" type="indirect"></relation>
<relation name="UMR 8104" active="#struct-441569" type="indirect"></relation>
<relation name="U1016" active="#struct-303623" type="indirect"></relation>
<relation active="#struct-189806" type="direct"></relation>
<relation active="#struct-300068" type="indirect"></relation>
<relation active="#struct-300123" type="indirect"></relation>
</listRelation>
<tutelles><tutelle active="#struct-106187" type="direct"><org type="laboratory" xml:id="struct-106187" status="VALID"> <idno type="IdRef">158485130</idno>
<idno type="RNSR">200217519N</idno>
<orgName>Institut Cochin</orgName>
<date type="start">2002</date>
<desc> <address> <addrLine>22 rue Méchain, 75014 Paris</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://cochin.inserm.fr/</ref>
</desc>
<listRelation> <relation active="#struct-303171" type="direct"></relation>
<relation active="#struct-301664" type="direct"></relation>
<relation name="UMR 8104" active="#struct-441569" type="direct"></relation>
<relation name="U1016" active="#struct-303623" type="direct"></relation>
</listRelation>
</org>
</tutelle>
<tutelle active="#struct-303171" type="indirect"><org type="institution" xml:id="struct-303171" status="VALID"> <orgName>Université Sorbonne Paris Cité</orgName>
<orgName type="acronym">USPC</orgName>
<desc> <address> <country key="FR"></country>
</address>
<ref type="url">http://www.sorbonne-paris-cite.fr/fr</ref>
</desc>
</org>
</tutelle>
<tutelle active="#struct-301664" type="indirect"><org type="institution" xml:id="struct-301664" status="VALID"><idno type="IdRef">026404788</idno>
<idno type="ISNI">0000 0001 2188 0914 </idno>
<orgName>Université Paris Descartes - Paris 5</orgName>
<orgName type="acronym">UPD5</orgName>
<desc><address><addrLine>12, rue de l'École de Médecine - 75270 Paris cedex 06</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.parisdescartes.fr/</ref>
</desc>
</org>
</tutelle>
<tutelle name="UMR 8104" active="#struct-441569" type="indirect"><org type="institution" xml:id="struct-441569" status="VALID"> <idno type="IdRef">02636817X</idno>
<idno type="ISNI">0000000122597504</idno>
<orgName>Centre National de la Recherche Scientifique</orgName>
<orgName type="acronym">CNRS</orgName>
<date type="start">1939-10-19</date>
<desc> <address> <country key="FR"></country>
</address>
<ref type="url">http://www.cnrs.fr/</ref>
</desc>
</org>
</tutelle>
<tutelle name="U1016" active="#struct-303623" type="indirect"><org type="institution" xml:id="struct-303623" status="VALID"> <idno type="IdRef">026388278</idno>
<orgName>Institut National de la Santé et de la Recherche Médicale</orgName>
<orgName type="acronym">INSERM</orgName>
<desc> <address> <addrLine>101, rue de Tolbiac, 75013 Paris </addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.inserm.fr</ref>
</desc>
</org>
</tutelle>
<tutelle active="#struct-189806" type="direct"><org type="laboratory" xml:id="struct-189806" status="VALID"> <orgName>Service de médecine interne et centre de référence des maladies rares [Cochin]</orgName>
<desc> <address> <addrLine>27, rue du Faubourg Saint-Jacques 75014 Paris</addrLine>
<country key="FR"></country>
</address>
</desc>
<listRelation> <relation active="#struct-300068" type="direct"></relation>
<relation active="#struct-300123" type="direct"></relation>
<relation active="#struct-301664" type="direct"></relation>
</listRelation>
</org>
</tutelle>
<tutelle active="#struct-300068" type="indirect"><org type="institution" xml:id="struct-300068" status="VALID"> <orgName>Assistance publique - Hôpitaux de Paris (AP-HP)</orgName>
<desc> <address> <country key="FR"></country>
</address>
</desc>
</org>
</tutelle>
<tutelle active="#struct-300123" type="indirect"><org type="institution" xml:id="struct-300123" status="VALID"> <orgName>CHU Cochin [APHP]</orgName>
<desc> <address> <addrLine>27 Rue du Faubourg Saint-Jacques 75014 Paris</addrLine>
<country key="FR"></country>
</address>
</desc>
</org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>France</country>
</affiliation>
</author>
<author><name sortKey="Le Gall, Morgane" sort="Le Gall, Morgane" uniqKey="Le Gall M" first="Morgane" last="Le Gall">Morgane Le Gall</name>
<affiliation wicri:level="1"><hal:affiliation type="researchteam" xml:id="struct-401129" status="INCOMING"> <orgName>Institut Cochin, INSERM U1016, CNRS UMR 8104, Université Paris Descartes</orgName>
<desc> <address> <country key="FR"></country>
</address>
</desc>
<listRelation> <relation active="#struct-106187" type="direct"></relation>
<relation active="#struct-303171" type="indirect"></relation>
<relation active="#struct-301664" type="indirect"></relation>
<relation name="UMR 8104" active="#struct-441569" type="indirect"></relation>
<relation name="U1016" active="#struct-303623" type="indirect"></relation>
<relation active="#struct-189806" type="direct"></relation>
<relation active="#struct-300068" type="indirect"></relation>
<relation active="#struct-300123" type="indirect"></relation>
</listRelation>
<tutelles><tutelle active="#struct-106187" type="direct"><org type="laboratory" xml:id="struct-106187" status="VALID"> <idno type="IdRef">158485130</idno>
<idno type="RNSR">200217519N</idno>
<orgName>Institut Cochin</orgName>
<date type="start">2002</date>
<desc> <address> <addrLine>22 rue Méchain, 75014 Paris</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://cochin.inserm.fr/</ref>
</desc>
<listRelation> <relation active="#struct-303171" type="direct"></relation>
<relation active="#struct-301664" type="direct"></relation>
<relation name="UMR 8104" active="#struct-441569" type="direct"></relation>
<relation name="U1016" active="#struct-303623" type="direct"></relation>
</listRelation>
</org>
</tutelle>
<tutelle active="#struct-303171" type="indirect"><org type="institution" xml:id="struct-303171" status="VALID"> <orgName>Université Sorbonne Paris Cité</orgName>
<orgName type="acronym">USPC</orgName>
<desc> <address> <country key="FR"></country>
</address>
<ref type="url">http://www.sorbonne-paris-cite.fr/fr</ref>
</desc>
</org>
</tutelle>
<tutelle active="#struct-301664" type="indirect"><org type="institution" xml:id="struct-301664" status="VALID"><idno type="IdRef">026404788</idno>
<idno type="ISNI">0000 0001 2188 0914 </idno>
<orgName>Université Paris Descartes - Paris 5</orgName>
<orgName type="acronym">UPD5</orgName>
<desc><address><addrLine>12, rue de l'École de Médecine - 75270 Paris cedex 06</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.parisdescartes.fr/</ref>
</desc>
</org>
</tutelle>
<tutelle name="UMR 8104" active="#struct-441569" type="indirect"><org type="institution" xml:id="struct-441569" status="VALID"> <idno type="IdRef">02636817X</idno>
<idno type="ISNI">0000000122597504</idno>
<orgName>Centre National de la Recherche Scientifique</orgName>
<orgName type="acronym">CNRS</orgName>
<date type="start">1939-10-19</date>
<desc> <address> <country key="FR"></country>
</address>
<ref type="url">http://www.cnrs.fr/</ref>
</desc>
</org>
</tutelle>
<tutelle name="U1016" active="#struct-303623" type="indirect"><org type="institution" xml:id="struct-303623" status="VALID"> <idno type="IdRef">026388278</idno>
<orgName>Institut National de la Santé et de la Recherche Médicale</orgName>
<orgName type="acronym">INSERM</orgName>
<desc> <address> <addrLine>101, rue de Tolbiac, 75013 Paris </addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.inserm.fr</ref>
</desc>
</org>
</tutelle>
<tutelle active="#struct-189806" type="direct"><org type="laboratory" xml:id="struct-189806" status="VALID"> <orgName>Service de médecine interne et centre de référence des maladies rares [Cochin]</orgName>
<desc> <address> <addrLine>27, rue du Faubourg Saint-Jacques 75014 Paris</addrLine>
<country key="FR"></country>
</address>
</desc>
<listRelation> <relation active="#struct-300068" type="direct"></relation>
<relation active="#struct-300123" type="direct"></relation>
<relation active="#struct-301664" type="direct"></relation>
</listRelation>
</org>
</tutelle>
<tutelle active="#struct-300068" type="indirect"><org type="institution" xml:id="struct-300068" status="VALID"> <orgName>Assistance publique - Hôpitaux de Paris (AP-HP)</orgName>
<desc> <address> <country key="FR"></country>
</address>
</desc>
</org>
</tutelle>
<tutelle active="#struct-300123" type="indirect"><org type="institution" xml:id="struct-300123" status="VALID"> <orgName>CHU Cochin [APHP]</orgName>
<desc> <address> <addrLine>27 Rue du Faubourg Saint-Jacques 75014 Paris</addrLine>
<country key="FR"></country>
</address>
</desc>
</org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>France</country>
</affiliation>
</author>
<author><name sortKey="Clary, Guilhem" sort="Clary, Guilhem" uniqKey="Clary G" first="Guilhem" last="Clary">Guilhem Clary</name>
<affiliation wicri:level="1"><hal:affiliation type="researchteam" xml:id="struct-401129" status="INCOMING"> <orgName>Institut Cochin, INSERM U1016, CNRS UMR 8104, Université Paris Descartes</orgName>
<desc> <address> <country key="FR"></country>
</address>
</desc>
<listRelation> <relation active="#struct-106187" type="direct"></relation>
<relation active="#struct-303171" type="indirect"></relation>
<relation active="#struct-301664" type="indirect"></relation>
<relation name="UMR 8104" active="#struct-441569" type="indirect"></relation>
<relation name="U1016" active="#struct-303623" type="indirect"></relation>
<relation active="#struct-189806" type="direct"></relation>
<relation active="#struct-300068" type="indirect"></relation>
<relation active="#struct-300123" type="indirect"></relation>
</listRelation>
<tutelles><tutelle active="#struct-106187" type="direct"><org type="laboratory" xml:id="struct-106187" status="VALID"> <idno type="IdRef">158485130</idno>
<idno type="RNSR">200217519N</idno>
<orgName>Institut Cochin</orgName>
<date type="start">2002</date>
<desc> <address> <addrLine>22 rue Méchain, 75014 Paris</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://cochin.inserm.fr/</ref>
</desc>
<listRelation> <relation active="#struct-303171" type="direct"></relation>
<relation active="#struct-301664" type="direct"></relation>
<relation name="UMR 8104" active="#struct-441569" type="direct"></relation>
<relation name="U1016" active="#struct-303623" type="direct"></relation>
</listRelation>
</org>
</tutelle>
<tutelle active="#struct-303171" type="indirect"><org type="institution" xml:id="struct-303171" status="VALID"> <orgName>Université Sorbonne Paris Cité</orgName>
<orgName type="acronym">USPC</orgName>
<desc> <address> <country key="FR"></country>
</address>
<ref type="url">http://www.sorbonne-paris-cite.fr/fr</ref>
</desc>
</org>
</tutelle>
<tutelle active="#struct-301664" type="indirect"><org type="institution" xml:id="struct-301664" status="VALID"><idno type="IdRef">026404788</idno>
<idno type="ISNI">0000 0001 2188 0914 </idno>
<orgName>Université Paris Descartes - Paris 5</orgName>
<orgName type="acronym">UPD5</orgName>
<desc><address><addrLine>12, rue de l'École de Médecine - 75270 Paris cedex 06</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.parisdescartes.fr/</ref>
</desc>
</org>
</tutelle>
<tutelle name="UMR 8104" active="#struct-441569" type="indirect"><org type="institution" xml:id="struct-441569" status="VALID"> <idno type="IdRef">02636817X</idno>
<idno type="ISNI">0000000122597504</idno>
<orgName>Centre National de la Recherche Scientifique</orgName>
<orgName type="acronym">CNRS</orgName>
<date type="start">1939-10-19</date>
<desc> <address> <country key="FR"></country>
</address>
<ref type="url">http://www.cnrs.fr/</ref>
</desc>
</org>
</tutelle>
<tutelle name="U1016" active="#struct-303623" type="indirect"><org type="institution" xml:id="struct-303623" status="VALID"> <idno type="IdRef">026388278</idno>
<orgName>Institut National de la Santé et de la Recherche Médicale</orgName>
<orgName type="acronym">INSERM</orgName>
<desc> <address> <addrLine>101, rue de Tolbiac, 75013 Paris </addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.inserm.fr</ref>
</desc>
</org>
</tutelle>
<tutelle active="#struct-189806" type="direct"><org type="laboratory" xml:id="struct-189806" status="VALID"> <orgName>Service de médecine interne et centre de référence des maladies rares [Cochin]</orgName>
<desc> <address> <addrLine>27, rue du Faubourg Saint-Jacques 75014 Paris</addrLine>
<country key="FR"></country>
</address>
</desc>
<listRelation> <relation active="#struct-300068" type="direct"></relation>
<relation active="#struct-300123" type="direct"></relation>
<relation active="#struct-301664" type="direct"></relation>
</listRelation>
</org>
</tutelle>
<tutelle active="#struct-300068" type="indirect"><org type="institution" xml:id="struct-300068" status="VALID"> <orgName>Assistance publique - Hôpitaux de Paris (AP-HP)</orgName>
<desc> <address> <country key="FR"></country>
</address>
</desc>
</org>
</tutelle>
<tutelle active="#struct-300123" type="indirect"><org type="institution" xml:id="struct-300123" status="VALID"> <orgName>CHU Cochin [APHP]</orgName>
<desc> <address> <addrLine>27 Rue du Faubourg Saint-Jacques 75014 Paris</addrLine>
<country key="FR"></country>
</address>
</desc>
</org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>France</country>
</affiliation>
</author>
<author><name sortKey="Melki, Ronald" sort="Melki, Ronald" uniqKey="Melki R" first="Ronald" last="Melki">Ronald Melki</name>
<affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-418266" status="VALID"> <orgName>Institut des Neurosciences de Paris-Saclay</orgName>
<orgName type="acronym">Neuro-PSI</orgName>
<desc> <address> <addrLine>Centre National de la Recherche Scientifique, Unité Mixte de Recherche-9197 Université Paris-Sudbâtiment 32 Campus de Recherche de Gif-sur-Yvette1, avenue de la Terrasse91198 Gif-sur-Yvette</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://neuro-psi.fr</ref>
</desc>
<listRelation> <relation active="#struct-92966" type="direct"></relation>
<relation name="UMR9197" active="#struct-441569" type="direct"></relation>
</listRelation>
<tutelles><tutelle active="#struct-92966" type="direct"><org type="institution" xml:id="struct-92966" status="VALID"> <orgName>Université Paris-Sud - Paris 11</orgName>
<orgName type="acronym">UP11</orgName>
<desc> <address> <addrLine>Bâtiment 300 - 91405 Orsay cedex</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.u-psud.fr/</ref>
</desc>
</org>
</tutelle>
<tutelle name="UMR9197" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID"> <idno type="IdRef">02636817X</idno>
<idno type="ISNI">0000000122597504</idno>
<orgName>Centre National de la Recherche Scientifique</orgName>
<orgName type="acronym">CNRS</orgName>
<date type="start">1939-10-19</date>
<desc> <address> <country key="FR"></country>
</address>
<ref type="url">http://www.cnrs.fr/</ref>
</desc>
</org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>France</country>
</affiliation>
</author>
<author><name sortKey="Redeker, Virginie" sort="Redeker, Virginie" uniqKey="Redeker V" first="Virginie" last="Redeker">Virginie Redeker</name>
<affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-418266" status="VALID"> <orgName>Institut des Neurosciences de Paris-Saclay</orgName>
<orgName type="acronym">Neuro-PSI</orgName>
<desc> <address> <addrLine>Centre National de la Recherche Scientifique, Unité Mixte de Recherche-9197 Université Paris-Sudbâtiment 32 Campus de Recherche de Gif-sur-Yvette1, avenue de la Terrasse91198 Gif-sur-Yvette</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://neuro-psi.fr</ref>
</desc>
<listRelation> <relation active="#struct-92966" type="direct"></relation>
<relation name="UMR9197" active="#struct-441569" type="direct"></relation>
</listRelation>
<tutelles><tutelle active="#struct-92966" type="direct"><org type="institution" xml:id="struct-92966" status="VALID"> <orgName>Université Paris-Sud - Paris 11</orgName>
<orgName type="acronym">UP11</orgName>
<desc> <address> <addrLine>Bâtiment 300 - 91405 Orsay cedex</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.u-psud.fr/</ref>
</desc>
</org>
</tutelle>
<tutelle name="UMR9197" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID"> <idno type="IdRef">02636817X</idno>
<idno type="ISNI">0000000122597504</idno>
<orgName>Centre National de la Recherche Scientifique</orgName>
<orgName type="acronym">CNRS</orgName>
<date type="start">1939-10-19</date>
<desc> <address> <country key="FR"></country>
</address>
<ref type="url">http://www.cnrs.fr/</ref>
</desc>
</org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>France</country>
</affiliation>
</author>
</analytic>
<idno type="DOI">10.1016/j.bbagen.2015.10.007</idno>
<series><title level="j">BBA - Biochimica et Biophysica Acta</title>
<idno type="ISSN">0006-3002</idno>
<imprint><date type="datePub">2015-12-31</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">BACKGROUND:α-Synuclein (α-Syn) fibrils are the main constituent of Lewy bodies and a neuropathological hallmark of Parkinson's disease (PD). The propagation of α-Syn assemblies from cell to cell suggests that they are involved in PD progression. We previously showed that α-Syn fibrils are toxic because of their ability to bind and permeabilize cell membranes. Here, we document the cellular response in terms of proteome changes of SH-SY5Y cells exposed to exogenous α-Syn fibrils.METHODS:We compare the proteomes of cells of neuronal origin exposed or not either to oligomeric or fibrillar α-Syn using two dimensional differential in-gel electrophoresis (2D-DIGE) and mass spectrometry.RESULTS:Only α-Syn fibrils induce significant changes in the proteome of SH-SY5Y cells. In addition to proteins associated to apoptosis and toxicity, or proteins previously linked to neurodegenerative diseases, we report an overexpression of proteins involved in intracellular vesicle trafficking. We also report a remarkable increase in fibrillar α-Syn heterogeneity, mainly due to C-terminal truncations.CONCLUSIONS:Our results show that cells of neuronal origin adapt their proteome to exogenous α-Syn fibrils and actively modify those assemblies.GENERAL SIGNIFICANCE:Cells of neuronal origin adapt their proteome to exogenous toxic α-Syn fibrils and actively modify those assemblies. Our results bring insights into the cellular response and clearance events the cells implement to face the propagation of α-Syn assemblies associated to pathology.</div>
</front>
</TEI>
<hal api="V3"> <titleStmt> <title xml:lang="en">Cellular response of human neuroblastoma cells to α-synuclein fibrils, the main constituent of Lewy bodies.</title>
<author role="aut"> <persName> <forename type="first">Laura</forename>
<surname>Pieri</surname>
</persName>
<idno type="halauthorid">1147059</idno>
<affiliation ref="#struct-418266"></affiliation>
</author>
<author role="aut"> <persName> <forename type="first">Philippe</forename>
<surname>Chafey</surname>
</persName>
<idno type="halauthorid">482234</idno>
<affiliation ref="#struct-401129"></affiliation>
</author>
<author role="aut"> <persName> <forename type="first">Morgane</forename>
<surname>Le Gall</surname>
</persName>
<idno type="halauthorid">143328</idno>
<affiliation ref="#struct-401129"></affiliation>
</author>
<author role="aut"> <persName> <forename type="first">Guilhem</forename>
<surname>Clary</surname>
</persName>
<idno type="halauthorid">1231868</idno>
<affiliation ref="#struct-401129"></affiliation>
</author>
<author role="aut"> <persName> <forename type="first">Ronald</forename>
<surname>Melki</surname>
</persName>
<idno type="halauthorid">190086</idno>
<affiliation ref="#struct-418266"></affiliation>
</author>
<author role="aut"> <persName> <forename type="first">Virginie</forename>
<surname>Redeker</surname>
</persName>
<idno type="halauthorid">127279</idno>
<affiliation ref="#struct-418266"></affiliation>
</author>
<editor role="depositor"> <persName> <forename>Alain</forename>
<surname>PERIGNON</surname>
</persName>
<email type="md5">0915a8bc0b5a494fc86eb9724296445c</email>
<email type="domain">cnrs.fr</email>
</editor>
</titleStmt>
<editionStmt> <edition n="v1" type="current"> <date type="whenSubmitted">2015-12-08 17:51:50</date>
<date type="whenModified">2017-02-09 16:08:24</date>
<date type="whenReleased">2015-12-08 17:51:50</date>
<date type="whenProduced">2015-12-31</date>
</edition>
<respStmt> <resp>contributor</resp>
<name key="108746"> <persName> <forename>Alain</forename>
<surname>PERIGNON</surname>
</persName>
<email type="md5">0915a8bc0b5a494fc86eb9724296445c</email>
<email type="domain">cnrs.fr</email>
</name>
</respStmt>
</editionStmt>
<publicationStmt> <distributor>CCSD</distributor>
<idno type="halId">hal-01240193</idno>
<idno type="halUri">https://hal.archives-ouvertes.fr/hal-01240193</idno>
<idno type="halBibtex">pieri:hal-01240193</idno>
<idno type="halRefHtml">BBA - Biochimica et Biophysica Acta, Elsevier, 2015, 1860 (1 Pt A), pp.8-19. <10.1016/j.bbagen.2015.10.007></idno>
<idno type="halRef">BBA - Biochimica et Biophysica Acta, Elsevier, 2015, 1860 (1 Pt A), pp.8-19. <10.1016/j.bbagen.2015.10.007></idno>
</publicationStmt>
<seriesStmt> <idno type="stamp" n="CNRS">CNRS - Centre national de la recherche scientifique</idno>
<idno type="stamp" n="APHP" p="INSERM">AP-HP</idno>
<idno type="stamp" n="UNIV-PARIS5">Université Paris Descartes (Paris 5)</idno>
<idno type="stamp" n="USPC">Université Sorbonne Paris Cité</idno>
<idno type="stamp" n="UNIV-PSUD-SACLAY" p="UNIV-PARIS-SACLAY">Université Paris Sud pour Paris Saclay</idno>
</seriesStmt>
<notesStmt> <note type="audience" n="2">International</note>
<note type="popular" n="0">No</note>
<note type="peer" n="1">Yes</note>
</notesStmt>
<sourceDesc> <biblStruct> <analytic> <title xml:lang="en">Cellular response of human neuroblastoma cells to α-synuclein fibrils, the main constituent of Lewy bodies.</title>
<author role="aut"> <persName> <forename type="first">Laura</forename>
<surname>Pieri</surname>
</persName>
<idno type="halauthorid">1147059</idno>
<affiliation ref="#struct-418266"></affiliation>
</author>
<author role="aut"> <persName> <forename type="first">Philippe</forename>
<surname>Chafey</surname>
</persName>
<idno type="halauthorid">482234</idno>
<affiliation ref="#struct-401129"></affiliation>
</author>
<author role="aut"> <persName> <forename type="first">Morgane</forename>
<surname>Le Gall</surname>
</persName>
<idno type="halauthorid">143328</idno>
<affiliation ref="#struct-401129"></affiliation>
</author>
<author role="aut"> <persName> <forename type="first">Guilhem</forename>
<surname>Clary</surname>
</persName>
<idno type="halauthorid">1231868</idno>
<affiliation ref="#struct-401129"></affiliation>
</author>
<author role="aut"> <persName> <forename type="first">Ronald</forename>
<surname>Melki</surname>
</persName>
<idno type="halauthorid">190086</idno>
<affiliation ref="#struct-418266"></affiliation>
</author>
<author role="aut"> <persName> <forename type="first">Virginie</forename>
<surname>Redeker</surname>
</persName>
<idno type="halauthorid">127279</idno>
<affiliation ref="#struct-418266"></affiliation>
</author>
</analytic>
<monogr> <idno type="halJournalId" status="VALID">11108</idno>
<idno type="issn">0006-3002</idno>
<title level="j">BBA - Biochimica et Biophysica Acta</title>
<imprint> <publisher>Elsevier</publisher>
<biblScope unit="volume">1860</biblScope>
<biblScope unit="issue">1 Pt A</biblScope>
<biblScope unit="pp">8-19</biblScope>
<date type="datePub">2015-12-31</date>
</imprint>
</monogr>
<idno type="doi">10.1016/j.bbagen.2015.10.007</idno>
<idno type="pubmed">26468903</idno>
</biblStruct>
</sourceDesc>
<profileDesc> <langUsage> <language ident="en">English</language>
</langUsage>
<textClass> <classCode scheme="halDomain" n="sdv.neu.nb">Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology</classCode>
<classCode scheme="halDomain" n="sdv.neu.pc">Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Psychology and behavior</classCode>
<classCode scheme="halDomain" n="sdv.neu.sc">Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Cognitive Sciences</classCode>
<classCode scheme="halTypology" n="ART">Journal articles</classCode>
</textClass>
<abstract xml:lang="en">BACKGROUND:α-Synuclein (α-Syn) fibrils are the main constituent of Lewy bodies and a neuropathological hallmark of Parkinson's disease (PD). The propagation of α-Syn assemblies from cell to cell suggests that they are involved in PD progression. We previously showed that α-Syn fibrils are toxic because of their ability to bind and permeabilize cell membranes. Here, we document the cellular response in terms of proteome changes of SH-SY5Y cells exposed to exogenous α-Syn fibrils.METHODS:We compare the proteomes of cells of neuronal origin exposed or not either to oligomeric or fibrillar α-Syn using two dimensional differential in-gel electrophoresis (2D-DIGE) and mass spectrometry.RESULTS:Only α-Syn fibrils induce significant changes in the proteome of SH-SY5Y cells. In addition to proteins associated to apoptosis and toxicity, or proteins previously linked to neurodegenerative diseases, we report an overexpression of proteins involved in intracellular vesicle trafficking. We also report a remarkable increase in fibrillar α-Syn heterogeneity, mainly due to C-terminal truncations.CONCLUSIONS:Our results show that cells of neuronal origin adapt their proteome to exogenous α-Syn fibrils and actively modify those assemblies.GENERAL SIGNIFICANCE:Cells of neuronal origin adapt their proteome to exogenous toxic α-Syn fibrils and actively modify those assemblies. Our results bring insights into the cellular response and clearance events the cells implement to face the propagation of α-Syn assemblies associated to pathology.</abstract>
</profileDesc>
</hal>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/ParkinsonFranceV1/Data/Hal/Checkpoint
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000109 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Hal/Checkpoint/biblio.hfd -nk 000109 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Sante |area= ParkinsonFranceV1 |flux= Hal |étape= Checkpoint |type= RBID |clé= Hal:hal-01240193 |texte= Cellular response of human neuroblastoma cells to α-synuclein fibrils, the main constituent of Lewy bodies. }}
![]() | This area was generated with Dilib version V0.6.29. | ![]() |