Development of an enzyme-linked immunoassay for the quantitation of influenza haemagglutinin: an alternative method to single radial immunodiffusion.
Identifieur interne : 000C95 ( PubMed/Corpus ); précédent : 000C94; suivant : 000C96Development of an enzyme-linked immunoassay for the quantitation of influenza haemagglutinin: an alternative method to single radial immunodiffusion.
Auteurs : Jesse Bodle ; Erin E. Verity ; Chi Ong ; Kirsten Vandenberg ; Robert Shaw ; Ian G. Barr ; Steven RockmanSource :
- Influenza and other respiratory viruses [ 1750-2659 ] ; 2013.
English descriptors
- KwdEn :
- Antibodies, Monoclonal (isolation & purification), Enzyme-Linked Immunosorbent Assay (methods), Hemagglutinin Glycoproteins, Influenza Virus (analysis), Horseradish Peroxidase (metabolism), Humans, Immunodiffusion (methods), Influenza Vaccines (chemistry), Influenza Vaccines (standards), Technology, Pharmaceutical (methods).
- MESH :
- chemical , analysis : Hemagglutinin Glycoproteins, Influenza Virus.
- chemical , chemistry : Influenza Vaccines.
- chemical , isolation & purification : Antibodies, Monoclonal.
- chemical , metabolism : Horseradish Peroxidase.
- methods : Enzyme-Linked Immunosorbent Assay, Immunodiffusion, Technology, Pharmaceutical.
- chemical , standards : Influenza Vaccines.
- Humans.
Abstract
The current method used to measure haemagglutinin (HA) content for influenza vaccine formulation, single radial immunodiffusion (SRID), is lengthy and relies on the availability of matched standardised homologous reagents. The 2009 influenza pandemic highlighted the need to develop alternate assays that are able to rapidly quantitate HA antigen for vaccine formulation.
DOI: 10.1111/j.1750-2659.2012.00375.x
PubMed: 22583601
Links to Exploration step
pubmed:22583601Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Development of an enzyme-linked immunoassay for the quantitation of influenza haemagglutinin: an alternative method to single radial immunodiffusion.</title><author><name sortKey="Bodle, Jesse" sort="Bodle, Jesse" uniqKey="Bodle J" first="Jesse" last="Bodle">Jesse Bodle</name><affiliation><nlm:affiliation>CSL Ltd, Parkville, Vic., Australia.</nlm:affiliation></affiliation></author><author><name sortKey="Verity, Erin E" sort="Verity, Erin E" uniqKey="Verity E" first="Erin E" last="Verity">Erin E. Verity</name></author><author><name sortKey="Ong, Chi" sort="Ong, Chi" uniqKey="Ong C" first="Chi" last="Ong">Chi Ong</name></author><author><name sortKey="Vandenberg, Kirsten" sort="Vandenberg, Kirsten" uniqKey="Vandenberg K" first="Kirsten" last="Vandenberg">Kirsten Vandenberg</name></author><author><name sortKey="Shaw, Robert" sort="Shaw, Robert" uniqKey="Shaw R" first="Robert" last="Shaw">Robert Shaw</name></author><author><name sortKey="Barr, Ian G" sort="Barr, Ian G" uniqKey="Barr I" first="Ian G" last="Barr">Ian G. Barr</name></author><author><name sortKey="Rockman, Steven" sort="Rockman, Steven" uniqKey="Rockman S" first="Steven" last="Rockman">Steven Rockman</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2013">2013</date><idno type="RBID">pubmed:22583601</idno><idno type="pmid">22583601</idno><idno type="doi">10.1111/j.1750-2659.2012.00375.x</idno><idno type="wicri:Area/PubMed/Corpus">000C95</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000C95</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Development of an enzyme-linked immunoassay for the quantitation of influenza haemagglutinin: an alternative method to single radial immunodiffusion.</title><author><name sortKey="Bodle, Jesse" sort="Bodle, Jesse" uniqKey="Bodle J" first="Jesse" last="Bodle">Jesse Bodle</name><affiliation><nlm:affiliation>CSL Ltd, Parkville, Vic., Australia.</nlm:affiliation></affiliation></author><author><name sortKey="Verity, Erin E" sort="Verity, Erin E" uniqKey="Verity E" first="Erin E" last="Verity">Erin E. Verity</name></author><author><name sortKey="Ong, Chi" sort="Ong, Chi" uniqKey="Ong C" first="Chi" last="Ong">Chi Ong</name></author><author><name sortKey="Vandenberg, Kirsten" sort="Vandenberg, Kirsten" uniqKey="Vandenberg K" first="Kirsten" last="Vandenberg">Kirsten Vandenberg</name></author><author><name sortKey="Shaw, Robert" sort="Shaw, Robert" uniqKey="Shaw R" first="Robert" last="Shaw">Robert Shaw</name></author><author><name sortKey="Barr, Ian G" sort="Barr, Ian G" uniqKey="Barr I" first="Ian G" last="Barr">Ian G. Barr</name></author><author><name sortKey="Rockman, Steven" sort="Rockman, Steven" uniqKey="Rockman S" first="Steven" last="Rockman">Steven Rockman</name></author></analytic><series><title level="j">Influenza and other respiratory viruses</title><idno type="eISSN">1750-2659</idno><imprint><date when="2013" type="published">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antibodies, Monoclonal (isolation & purification)</term><term>Enzyme-Linked Immunosorbent Assay (methods)</term><term>Hemagglutinin Glycoproteins, Influenza Virus (analysis)</term><term>Horseradish Peroxidase (metabolism)</term><term>Humans</term><term>Immunodiffusion (methods)</term><term>Influenza Vaccines (chemistry)</term><term>Influenza Vaccines (standards)</term><term>Technology, Pharmaceutical (methods)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Hemagglutinin Glycoproteins, Influenza Virus</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Influenza Vaccines</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Antibodies, Monoclonal</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Horseradish Peroxidase</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Enzyme-Linked Immunosorbent Assay</term><term>Immunodiffusion</term><term>Technology, Pharmaceutical</term></keywords><keywords scheme="MESH" type="chemical" qualifier="standards" xml:lang="en"><term>Influenza Vaccines</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Humans</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The current method used to measure haemagglutinin (HA) content for influenza vaccine formulation, single radial immunodiffusion (SRID), is lengthy and relies on the availability of matched standardised homologous reagents. The 2009 influenza pandemic highlighted the need to develop alternate assays that are able to rapidly quantitate HA antigen for vaccine formulation.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">22583601</PMID><DateCompleted><Year>2013</Year><Month>07</Month><Day>29</Day></DateCompleted><DateRevised><Year>2019</Year><Month>12</Month><Day>10</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1750-2659</ISSN><JournalIssue CitedMedium="Internet"><Volume>7</Volume><Issue>2</Issue><PubDate><Year>2013</Year><Month>Mar</Month></PubDate></JournalIssue><Title>Influenza and other respiratory viruses</Title><ISOAbbreviation>Influenza Other Respir Viruses</ISOAbbreviation></Journal><ArticleTitle>Development of an enzyme-linked immunoassay for the quantitation of influenza haemagglutinin: an alternative method to single radial immunodiffusion.</ArticleTitle><Pagination><MedlinePgn>191-200</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1111/j.1750-2659.2012.00375.x</ELocationID><Abstract><AbstractText Label="BACKGROUND" NlmCategory="BACKGROUND">The current method used to measure haemagglutinin (HA) content for influenza vaccine formulation, single radial immunodiffusion (SRID), is lengthy and relies on the availability of matched standardised homologous reagents. The 2009 influenza pandemic highlighted the need to develop alternate assays that are able to rapidly quantitate HA antigen for vaccine formulation.</AbstractText><AbstractText Label="OBJECTIVES" NlmCategory="OBJECTIVE">The aim of this work was to develop an enzyme-linked immunoassay (EIA) for the rapid quantitation of H1, H3, H5 and B influenza HA antigens.</AbstractText><AbstractText Label="METHODS" NlmCategory="METHODS">Monoclonal antibodies (mAbs) selected for haemagglutination inhibition (HAI) activity were conjugated with horseradish peroxidase and used to establish a capture-detection EIA for the quantitation of HA antigen. Results were compared with the appropriate reference SRID assays to investigate assay performance and utility.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">Quantitation of HA antigen by EIA correlated well with current reference SRID assays. EIA results showed equivalent precision and exhibited a similar capacity to detect HA antigen in virus samples that had been used in either stability or splitting studies, or subjected to physical or chemical stresses. EIA exhibited greater sensitivity than SRID and has the potential to be used in high-throughput applications.</AbstractText><AbstractText Label="CONCLUSIONS" NlmCategory="CONCLUSIONS">We demonstrated the utility of EIA as a suitable alternative to SRID for HA antigen quantitation and stability assessment. This approach would lead to earlier availability of both seasonal and pandemic vaccines, because of the extended cross-reactivity of reagents.</AbstractText><CopyrightInformation>© 2012 Blackwell Publishing Ltd.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Bodle</LastName><ForeName>Jesse</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>CSL Ltd, Parkville, Vic., Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Verity</LastName><ForeName>Erin E</ForeName><Initials>EE</Initials></Author><Author ValidYN="Y"><LastName>Ong</LastName><ForeName>Chi</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Vandenberg</LastName><ForeName>Kirsten</ForeName><Initials>K</Initials></Author><Author ValidYN="Y"><LastName>Shaw</LastName><ForeName>Robert</ForeName><Initials>R</Initials></Author><Author ValidYN="Y"><LastName>Barr</LastName><ForeName>Ian G</ForeName><Initials>IG</Initials></Author><Author ValidYN="Y"><LastName>Rockman</LastName><ForeName>Steven</ForeName><Initials>S</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D003160">Comparative Study</PublicationType><PublicationType UI="D023362">Evaluation Study</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2012</Year><Month>05</Month><Day>15</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Influenza Other Respir Viruses</MedlineTA><NlmUniqueID>101304007</NlmUniqueID><ISSNLinking>1750-2640</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000911">Antibodies, Monoclonal</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D019267">Hemagglutinin Glycoproteins, Influenza Virus</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D007252">Influenza Vaccines</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.11.1.-</RegistryNumber><NameOfSubstance UI="D006735">Horseradish Peroxidase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000911" MajorTopicYN="N">Antibodies, Monoclonal</DescriptorName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004797" MajorTopicYN="N">Enzyme-Linked Immunosorbent Assay</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D019267" MajorTopicYN="N">Hemagglutinin Glycoproteins, Influenza Virus</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006735" MajorTopicYN="N">Horseradish Peroxidase</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005779" MajorTopicYN="N">Immunodiffusion</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D007252" MajorTopicYN="N">Influenza Vaccines</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName><QualifierName UI="Q000592" MajorTopicYN="Y">standards</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013678" MajorTopicYN="N">Technology, Pharmaceutical</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2012</Year><Month>5</Month><Day>16</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2012</Year><Month>5</Month><Day>16</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2013</Year><Month>7</Month><Day>31</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">22583601</ArticleId><ArticleId IdType="doi">10.1111/j.1750-2659.2012.00375.x</ArticleId><ArticleId IdType="pmc">PMC5780761</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Vaccine. 2008 May 12;26(20):2510-20</Citation><ArticleIdList><ArticleId IdType="pubmed">18440105</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2008 Jul;82(13):6200-8</Citation><ArticleIdList><ArticleId IdType="pubmed">18417563</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 1981 Jan 29;289(5796):373-8</Citation><ArticleIdList><ArticleId IdType="pubmed">6162101</ArticleId></ArticleIdList></Reference><Reference><Citation>Dev Biol Stand. 1977 Jun 1-3;39:169-78</Citation><ArticleIdList><ArticleId IdType="pubmed">414948</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2007 Apr;81(7):3514-24</Citation><ArticleIdList><ArticleId IdType="pubmed">17251294</ArticleId></ArticleIdList></Reference><Reference><Citation>Dev Biol Stand. 1984;57:251-5</Citation><ArticleIdList><ArticleId IdType="pubmed">6526141</ArticleId></ArticleIdList></Reference><Reference><Citation>Bull World Health Organ. 1975;52(2):223-31</Citation><ArticleIdList><ArticleId IdType="pubmed">816480</ArticleId></ArticleIdList></Reference><Reference><Citation>Hybrid Hybridomics. 2002 Oct;21(5):333-8</Citation><ArticleIdList><ArticleId IdType="pubmed">12470475</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 1988 Jun 2;333(6172):426-31</Citation><ArticleIdList><ArticleId IdType="pubmed">3374584</ArticleId></ArticleIdList></Reference><Reference><Citation>Dev Biol Stand. 1977 Jun 1-3;39:193-200</Citation><ArticleIdList><ArticleId IdType="pubmed">414951</ArticleId></ArticleIdList></Reference><Reference><Citation>Influenza Other Respir Viruses. 2012 May;6(3):176-87</Citation><ArticleIdList><ArticleId IdType="pubmed">21902817</ArticleId></ArticleIdList></Reference><Reference><Citation>Virology. 2011 Aug 15;417(1):196-202</Citation><ArticleIdList><ArticleId IdType="pubmed">21722934</ArticleId></ArticleIdList></Reference><Reference><Citation>Vaccine. 2006 Apr 12;24(16):3137-44</Citation><ArticleIdList><ArticleId IdType="pubmed">16490287</ArticleId></ArticleIdList></Reference><Reference><Citation>Clin Vaccine Immunol. 2007 May;14(5):617-23</Citation><ArticleIdList><ArticleId IdType="pubmed">17344345</ArticleId></ArticleIdList></Reference><Reference><Citation>Virology. 2002 Oct 25;302(2):294-8</Citation><ArticleIdList><ArticleId IdType="pubmed">12441073</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biol Stand. 1980;8(4):289-96</Citation><ArticleIdList><ArticleId IdType="pubmed">6782106</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochem J. 2002 Aug 1;365(Pt 3):841-8</Citation><ArticleIdList><ArticleId IdType="pubmed">11994048</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/PandemieGrippaleV1/Data/PubMed/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000C95 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/PubMed/Corpus/biblio.hfd -nk 000C95 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= PandemieGrippaleV1
|flux= PubMed
|étape= Corpus
|type= RBID
|clé= pubmed:22583601
|texte= Development of an enzyme-linked immunoassay for the quantitation of influenza haemagglutinin: an alternative method to single radial immunodiffusion.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Corpus/RBID.i -Sk "pubmed:22583601" \
| HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Corpus/biblio.hfd \
| NlmPubMed2Wicri -a PandemieGrippaleV1
| This area was generated with Dilib version V0.6.34. |  |