Generation of human neutralizing monoclonal antibodies against the 2009 pandemic H1N1 virus from peripheral blood memory B lymphocytes.
Identifieur interne : 000974 ( PubMed/Corpus ); précédent : 000973; suivant : 000975Generation of human neutralizing monoclonal antibodies against the 2009 pandemic H1N1 virus from peripheral blood memory B lymphocytes.
Auteurs : Hao Wang ; Chi Ma ; Yanlai Lu ; Xu Ji ; Yongsheng Pang ; Fang Hua ; Lianxian Cui ; Denian Ba ; Wei HeSource :
- Cellular & molecular immunology [ 2042-0226 ] ; 2013.
English descriptors
- KwdEn :
- Antibodies, Monoclonal (biosynthesis), Antibodies, Monoclonal (blood), Antibodies, Monoclonal (isolation & purification), Antibodies, Neutralizing (biosynthesis), Antibodies, Neutralizing (blood), Antibodies, Neutralizing (isolation & purification), Antibodies, Viral (biosynthesis), Antibodies, Viral (blood), Antibodies, Viral (isolation & purification), B-Lymphocytes (immunology), B-Lymphocytes (metabolism), B-Lymphocytes (virology), China (epidemiology), Hemagglutination Inhibition Tests, Herpesvirus 4, Human (genetics), Herpesvirus 4, Human (immunology), High-Throughput Screening Assays, Humans, Immunologic Memory, Influenza A Virus, H1N1 Subtype (immunology), Influenza Vaccines (administration & dosage), Influenza, Human (blood), Influenza, Human (epidemiology), Influenza, Human (immunology), Influenza, Human (prevention & control), Neutralization Tests, Pandemics, Vaccination.
- MESH :
- chemical , administration & dosage : Influenza Vaccines.
- chemical , biosynthesis : Antibodies, Monoclonal, Antibodies, Neutralizing, Antibodies, Viral.
- chemical , blood : Antibodies, Monoclonal, Antibodies, Neutralizing, Antibodies, Viral.
- chemical , isolation & purification : Antibodies, Monoclonal, Antibodies, Neutralizing, Antibodies, Viral.
- geographic , epidemiology : China.
- blood : Influenza, Human.
- epidemiology : Influenza, Human.
- genetics : Herpesvirus 4, Human.
- immunology : B-Lymphocytes, Herpesvirus 4, Human, Influenza A Virus, H1N1 Subtype, Influenza, Human.
- metabolism : B-Lymphocytes.
- prevention & control : Influenza, Human.
- virology : B-Lymphocytes.
- Hemagglutination Inhibition Tests, High-Throughput Screening Assays, Humans, Immunologic Memory, Neutralization Tests, Pandemics, Vaccination.
Abstract
The 2009 H1N1 influenza pandemic demonstrated the significance of a global health threat to human beings. Although pandemic H1N1 vaccines have been rapidly developed, passive serotherapy may offer superior immediate protection against infections in children, the elderly and immune-compromised patients during an influenza pandemic. Here, we applied a novel strategy based on Epstein-Barr virus (EBV)-immortalized peripheral blood memory B cells to screen high viral neutralizing monoclonal antibodies (MAbs) from individuals vaccinated with the 2009 pandemic H1N1 vaccine PANFLU.1. Through a massive screen of 13 090 immortalized memory B-cell clones from three selected vaccinees, seven MAbs were identified with both high viral neutralizing capacities and hemagglutination inhibition (HAI) activities against the 2009 pandemic H1N1 viruses. These MAbs may have important clinical implications for passive serotherapy treatments of infected patients with severe respiratory syndrome, especially children, the elderly and immunodeficient individuals. Our successful strategy for generating high-affinity MAbs from EBV-immortalized peripheral blood memory B cells may also be applicable to other infectious or autoimmune diseases.
DOI: 10.1038/cmi.2013.25
PubMed: 23912783
Links to Exploration step
pubmed:23912783Le document en format XML
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scheme="MESH" type="chemical" qualifier="administration & dosage" xml:lang="en"><term>Influenza Vaccines</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Antibodies, Monoclonal</term><term>Antibodies, Neutralizing</term><term>Antibodies, Viral</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antibodies, Monoclonal</term><term>Antibodies, Neutralizing</term><term>Antibodies, Viral</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Antibodies, Monoclonal</term><term>Antibodies, Neutralizing</term><term>Antibodies, Viral</term></keywords><keywords scheme="MESH" type="geographic" qualifier="epidemiology" xml:lang="en"><term>China</term></keywords><keywords scheme="MESH" qualifier="blood" xml:lang="en"><term>Influenza, Human</term></keywords><keywords scheme="MESH" qualifier="epidemiology" xml:lang="en"><term>Influenza, Human</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Herpesvirus 4, Human</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>B-Lymphocytes</term><term>Herpesvirus 4, Human</term><term>Influenza A Virus, H1N1 Subtype</term><term>Influenza, Human</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>B-Lymphocytes</term></keywords><keywords scheme="MESH" qualifier="prevention & control" xml:lang="en"><term>Influenza, Human</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>B-Lymphocytes</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Hemagglutination Inhibition Tests</term><term>High-Throughput Screening Assays</term><term>Humans</term><term>Immunologic Memory</term><term>Neutralization Tests</term><term>Pandemics</term><term>Vaccination</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The 2009 H1N1 influenza pandemic demonstrated the significance of a global health threat to human beings. Although pandemic H1N1 vaccines have been rapidly developed, passive serotherapy may offer superior immediate protection against infections in children, the elderly and immune-compromised patients during an influenza pandemic. Here, we applied a novel strategy based on Epstein-Barr virus (EBV)-immortalized peripheral blood memory B cells to screen high viral neutralizing monoclonal antibodies (MAbs) from individuals vaccinated with the 2009 pandemic H1N1 vaccine PANFLU.1. Through a massive screen of 13 090 immortalized memory B-cell clones from three selected vaccinees, seven MAbs were identified with both high viral neutralizing capacities and hemagglutination inhibition (HAI) activities against the 2009 pandemic H1N1 viruses. These MAbs may have important clinical implications for passive serotherapy treatments of infected patients with severe respiratory syndrome, especially children, the elderly and immunodeficient individuals. Our successful strategy for generating high-affinity MAbs from EBV-immortalized peripheral blood memory B cells may also be applicable to other infectious or autoimmune diseases. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">23912783</PMID><DateCompleted><Year>2014</Year><Month>03</Month><Day>07</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">2042-0226</ISSN><JournalIssue CitedMedium="Internet"><Volume>10</Volume><Issue>5</Issue><PubDate><Year>2013</Year><Month>Sep</Month></PubDate></JournalIssue><Title>Cellular & molecular immunology</Title><ISOAbbreviation>Cell. Mol. Immunol.</ISOAbbreviation></Journal><ArticleTitle>Generation of human neutralizing monoclonal antibodies against the 2009 pandemic H1N1 virus from peripheral blood memory B lymphocytes.</ArticleTitle><Pagination><MedlinePgn>403-12</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1038/cmi.2013.25</ELocationID><Abstract><AbstractText>The 2009 H1N1 influenza pandemic demonstrated the significance of a global health threat to human beings. Although pandemic H1N1 vaccines have been rapidly developed, passive serotherapy may offer superior immediate protection against infections in children, the elderly and immune-compromised patients during an influenza pandemic. Here, we applied a novel strategy based on Epstein-Barr virus (EBV)-immortalized peripheral blood memory B cells to screen high viral neutralizing monoclonal antibodies (MAbs) from individuals vaccinated with the 2009 pandemic H1N1 vaccine PANFLU.1. Through a massive screen of 13 090 immortalized memory B-cell clones from three selected vaccinees, seven MAbs were identified with both high viral neutralizing capacities and hemagglutination inhibition (HAI) activities against the 2009 pandemic H1N1 viruses. These MAbs may have important clinical implications for passive serotherapy treatments of infected patients with severe respiratory syndrome, especially children, the elderly and immunodeficient individuals. Our successful strategy for generating high-affinity MAbs from EBV-immortalized peripheral blood memory B cells may also be applicable to other infectious or autoimmune diseases. </AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Hao</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Department of Immunology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and School of Basic Medicine, Peking UnionMedical College, State Key Laboratory of Medical Molecular Biology, Beijing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ma</LastName><ForeName>Chi</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Lu</LastName><ForeName>Yanlai</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Ji</LastName><ForeName>Xu</ForeName><Initials>X</Initials></Author><Author ValidYN="Y"><LastName>Pang</LastName><ForeName>Yongsheng</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Hua</LastName><ForeName>Fang</ForeName><Initials>F</Initials></Author><Author ValidYN="Y"><LastName>Cui</LastName><ForeName>Lianxian</ForeName><Initials>L</Initials></Author><Author ValidYN="Y"><LastName>Ba</LastName><ForeName>Denian</ForeName><Initials>D</Initials></Author><Author ValidYN="Y"><LastName>He</LastName><ForeName>Wei</ForeName><Initials>W</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2013</Year><Month>08</Month><Day>05</Day></ArticleDate></Article><MedlineJournalInfo><Country>China</Country><MedlineTA>Cell Mol 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IdType="doi">10.1038/cmi.2013.25</ArticleId><ArticleId IdType="pmc">PMC4003197</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>J Immunol Methods. 1999 Dec 10;231(1-2):11-23</Citation><ArticleIdList><ArticleId IdType="pubmed">10648924</ArticleId></ArticleIdList></Reference><Reference><Citation>J Paediatr Child Health. 2013 Mar;49(3):E183-8</Citation><ArticleIdList><ArticleId IdType="pubmed">23253033</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Rev Drug Discov. 2003 Jan;2(1):52-62</Citation><ArticleIdList><ArticleId IdType="pubmed">12509759</ArticleId></ArticleIdList></Reference><Reference><Citation>J Immunol. 2004 Aug 1;173(3):1978-86</Citation><ArticleIdList><ArticleId IdType="pubmed">15265932</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Med. 2004 Aug;10(8):871-5</Citation><ArticleIdList><ArticleId IdType="pubmed">15247913</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 1977 Sep 29;269(5627):420-2</Citation><ArticleIdList><ArticleId IdType="pubmed">198669</ArticleId></ArticleIdList></Reference><Reference><Citation>Hybridoma. 1982;1(3):323-8</Citation><ArticleIdList><ArticleId IdType="pubmed">6092264</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 1986 May 29-Jun 4;321(6069):522-5</Citation><ArticleIdList><ArticleId IdType="pubmed">3713831</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 1987 Dec;61(12):4033-7</Citation><ArticleIdList><ArticleId IdType="pubmed">2824833</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 1990 Dec 6;348(6301):552-4</Citation><ArticleIdList><ArticleId IdType="pubmed">2247164</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2005 Sep 6;102(36):12915-20</Citation><ArticleIdList><ArticleId IdType="pubmed">16118277</ArticleId></ArticleIdList></Reference><Reference><Citation>Hum Antibodies. 2006;15(4):115-24</Citation><ArticleIdList><ArticleId IdType="pubmed">17522433</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 2008 Sep 25;455(7212):532-6</Citation><ArticleIdList><ArticleId IdType="pubmed">18716625</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS One. 2008;3(12):e3942</Citation><ArticleIdList><ArticleId IdType="pubmed">19079604</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Struct Mol Biol. 2009 Mar;16(3):265-73</Citation><ArticleIdList><ArticleId IdType="pubmed">19234466</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 2009 Apr 10;324(5924):246-51</Citation><ArticleIdList><ArticleId IdType="pubmed">19251591</ArticleId></ArticleIdList></Reference><Reference><Citation>Influenza Other Respir Viruses. 2008 Nov;2(6):237-42</Citation><ArticleIdList><ArticleId IdType="pubmed">19453400</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 2009 Jul 10;325(5937):197-201</Citation><ArticleIdList><ArticleId IdType="pubmed">19465683</ArticleId></ArticleIdList></Reference><Reference><Citation>J Immunol. 2009 Sep 1;183(5):3177-87</Citation><ArticleIdList><ArticleId IdType="pubmed">19675172</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Med. 2009 Sep;15(9):1088-92</Citation><ArticleIdList><ArticleId IdType="pubmed">19684583</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biol Chem. 2009 Oct 2;284(40):27449-55</Citation><ArticleIdList><ArticleId IdType="pubmed">19666468</ArticleId></ArticleIdList></Reference><Reference><Citation>N Engl J Med. 2009 Nov 12;361(20):1945-52</Citation><ArticleIdList><ArticleId IdType="pubmed">19745214</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochem Biophys Res Commun. 2009 Dec 25;390(4):1305-8</Citation><ArticleIdList><ArticleId IdType="pubmed">19879239</ArticleId></ArticleIdList></Reference><Reference><Citation>Lancet. 2010 Jan 2;375(9708):56-66</Citation><ArticleIdList><ArticleId IdType="pubmed">20018364</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2010 Mar;84(6):3127-30</Citation><ArticleIdList><ArticleId IdType="pubmed">20042511</ArticleId></ArticleIdList></Reference><Reference><Citation>J Clin Invest. 2010 May;120(5):1663-73</Citation><ArticleIdList><ArticleId IdType="pubmed">20389023</ArticleId></ArticleIdList></Reference><Reference><Citation>N Engl J Med. 2010 Dec 16;363(25):2416-23</Citation><ArticleIdList><ArticleId IdType="pubmed">21158658</ArticleId></ArticleIdList></Reference><Reference><Citation>Protein Cell. 2010 Apr;1(4):319-30</Citation><ArticleIdList><ArticleId IdType="pubmed">21203944</ArticleId></ArticleIdList></Reference><Reference><Citation>J Exp Med. 2011 Jan 17;208(1):181-93</Citation><ArticleIdList><ArticleId IdType="pubmed">21220454</ArticleId></ArticleIdList></Reference><Reference><Citation>Immunol Lett. 2011 Mar 30;135(1-2):59-63</Citation><ArticleIdList><ArticleId IdType="pubmed">20923686</ArticleId></ArticleIdList></Reference><Reference><Citation>Influenza Other Respir Viruses. 2009 Sep;3(5):207-13</Citation><ArticleIdList><ArticleId IdType="pubmed">19768134</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 2011 Aug 12;333(6044):843-50</Citation><ArticleIdList><ArticleId IdType="pubmed">21737702</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 2011 Aug 12;333(6044):850-6</Citation><ArticleIdList><ArticleId IdType="pubmed">21798894</ArticleId></ArticleIdList></Reference><Reference><Citation>Arch Virol. 2011 Oct;156(10):1803-12</Citation><ArticleIdList><ArticleId IdType="pubmed">21744000</ArticleId></ArticleIdList></Reference><Reference><Citation>Cell Mol Immunol. 2011 Nov;8(6):462-8</Citation><ArticleIdList><ArticleId IdType="pubmed">21946434</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2011 Dec;85(24):13463-7</Citation><ArticleIdList><ArticleId IdType="pubmed">21994454</ArticleId></ArticleIdList></Reference><Reference><Citation>Curr Opin Virol. 2012 Apr;2(2):134-41</Citation><ArticleIdList><ArticleId IdType="pubmed">22482710</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2012 Jun 5;109(23):9047-52</Citation><ArticleIdList><ArticleId IdType="pubmed">22615367</ArticleId></ArticleIdList></Reference><Reference><Citation>Cell Mol Immunol. 2012 Sep;9(5):373-4</Citation><ArticleIdList><ArticleId IdType="pubmed">22885525</ArticleId></ArticleIdList></Reference><Reference><Citation>Cell Mol Immunol. 2013 Jan;10(1):50-7</Citation><ArticleIdList><ArticleId IdType="pubmed">23064104</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2001 Feb 13;98(4):1799-804</Citation><ArticleIdList><ArticleId IdType="pubmed">11172031</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Corpus/RBID.i -Sk "pubmed:23912783" \
| HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Corpus/biblio.hfd \
| NlmPubMed2Wicri -a PandemieGrippaleV1
| This area was generated with Dilib version V0.6.34. |  |