Microneedle Vaccination with Stabilized Recombinant Influenza Virus Hemagglutinin Induces Improved Protective Immunity ▿
Identifieur interne : 000675 ( Pmc/Curation ); précédent : 000674; suivant : 000676Microneedle Vaccination with Stabilized Recombinant Influenza Virus Hemagglutinin Induces Improved Protective Immunity ▿
Auteurs : William C. Weldon [États-Unis] ; Maria P. Martin [États-Unis] ; Vladimir Zarnitsyn [États-Unis] ; Baozhong Wang [États-Unis] ; Dimitrios Koutsonanos [États-Unis] ; Ioanna Skountzou [États-Unis] ; Mark R. Prausnitz [États-Unis] ; Richard W. Compans [États-Unis]Source :
- Clinical and Vaccine Immunology : CVI [ 1556-6811 ] ; 2011.
Abstract
The emergence of the swine-origin 2009 influenza pandemic illustrates the need for improved vaccine production and delivery strategies. Skin-based immunization represents an attractive alternative to traditional hypodermic needle vaccination routes. Microneedles (MNs) can deliver vaccine to the epidermis and dermis, which are rich in antigen-presenting cells (APC) such as Langerhans cells and dermal dendritic cells. Previous studies using coated or dissolvable microneedles emphasized the use of inactivated influenza virus or virus-like particles as skin-based vaccines. However, most currently available influenza vaccines consist of solubilized viral protein antigens. Here we test the hypothesis that a recombinant subunit influenza vaccine can be delivered to the skin by coated microneedles and can induce protective immunity. We found that mice vaccinated via MN delivery with a stabilized recombinant trimeric soluble hemagglutinin (sHA) derived from A/Aichi/2/68 (H3) virus had significantly higher immune responses than did mice vaccinated with unmodified sHA. These mice were fully protected against a lethal challenge with influenza virus. Analysis of postchallenge lung titers showed that MN-immunized mice had completely cleared the virus from their lungs, in contrast to mice given the same vaccine by a standard subcutaneous route. In addition, we observed a higher ratio of antigen-specific Th1 cells in trimeric sHA-vaccinated mice and a greater mucosal antibody response. Our data therefore demonstrate the improved efficacy of a skin-based recombinant subunit influenza vaccine and emphasize the advantage of this route of vaccination for a protein subunit vaccine.
Url:
DOI: 10.1128/CVI.00435-10
PubMed: 21288996
PubMed Central: 3122571
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: Pour aller vers cette notice dans l'étape Curation :000675
Links to Exploration step
PMC:3122571Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Microneedle Vaccination with Stabilized Recombinant Influenza Virus Hemagglutinin Induces Improved Protective Immunity
<xref ref-type="fn" rid="FN1"><sup>▿</sup>
</xref>
</title>
<author><name sortKey="Weldon, William C" sort="Weldon, William C" uniqKey="Weldon W" first="William C." last="Weldon">William C. Weldon</name>
<affiliation wicri:level="2"><nlm:aff id="aff1">Department of Microbiology and Immunology and Emory Vaccine Center, Emory University School of Medicine, 1510 Clifton Road, Atlanta, Georgia 30322</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Géorgie (États-Unis)</region>
</placeName>
<wicri:cityArea>Department of Microbiology and Immunology and Emory Vaccine Center, Emory University School of Medicine, 1510 Clifton Road, Atlanta</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Martin, Maria P" sort="Martin, Maria P" uniqKey="Martin M" first="Maria P." last="Martin">Maria P. Martin</name>
<affiliation wicri:level="2"><nlm:aff id="aff1">Department of Microbiology and Immunology and Emory Vaccine Center, Emory University School of Medicine, 1510 Clifton Road, Atlanta, Georgia 30322</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Géorgie (États-Unis)</region>
</placeName>
<wicri:cityArea>Department of Microbiology and Immunology and Emory Vaccine Center, Emory University School of Medicine, 1510 Clifton Road, Atlanta</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Zarnitsyn, Vladimir" sort="Zarnitsyn, Vladimir" uniqKey="Zarnitsyn V" first="Vladimir" last="Zarnitsyn">Vladimir Zarnitsyn</name>
<affiliation wicri:level="2"><nlm:aff id="aff2">Wallace Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, Georgia 30332</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Géorgie (États-Unis)</region>
</placeName>
<wicri:cityArea>Wallace Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Wang, Baozhong" sort="Wang, Baozhong" uniqKey="Wang B" first="Baozhong" last="Wang">Baozhong Wang</name>
<affiliation wicri:level="2"><nlm:aff id="aff1">Department of Microbiology and Immunology and Emory Vaccine Center, Emory University School of Medicine, 1510 Clifton Road, Atlanta, Georgia 30322</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Géorgie (États-Unis)</region>
</placeName>
<wicri:cityArea>Department of Microbiology and Immunology and Emory Vaccine Center, Emory University School of Medicine, 1510 Clifton Road, Atlanta</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Koutsonanos, Dimitrios" sort="Koutsonanos, Dimitrios" uniqKey="Koutsonanos D" first="Dimitrios" last="Koutsonanos">Dimitrios Koutsonanos</name>
<affiliation wicri:level="2"><nlm:aff id="aff1">Department of Microbiology and Immunology and Emory Vaccine Center, Emory University School of Medicine, 1510 Clifton Road, Atlanta, Georgia 30322</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Géorgie (États-Unis)</region>
</placeName>
<wicri:cityArea>Department of Microbiology and Immunology and Emory Vaccine Center, Emory University School of Medicine, 1510 Clifton Road, Atlanta</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Skountzou, Ioanna" sort="Skountzou, Ioanna" uniqKey="Skountzou I" first="Ioanna" last="Skountzou">Ioanna Skountzou</name>
<affiliation wicri:level="2"><nlm:aff id="aff1">Department of Microbiology and Immunology and Emory Vaccine Center, Emory University School of Medicine, 1510 Clifton Road, Atlanta, Georgia 30322</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Géorgie (États-Unis)</region>
</placeName>
<wicri:cityArea>Department of Microbiology and Immunology and Emory Vaccine Center, Emory University School of Medicine, 1510 Clifton Road, Atlanta</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Prausnitz, Mark R" sort="Prausnitz, Mark R" uniqKey="Prausnitz M" first="Mark R." last="Prausnitz">Mark R. Prausnitz</name>
<affiliation wicri:level="2"><nlm:aff id="aff2">Wallace Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, Georgia 30332</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Géorgie (États-Unis)</region>
</placeName>
<wicri:cityArea>Wallace Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Compans, Richard W" sort="Compans, Richard W" uniqKey="Compans R" first="Richard W." last="Compans">Richard W. Compans</name>
<affiliation wicri:level="2"><nlm:aff id="aff1">Department of Microbiology and Immunology and Emory Vaccine Center, Emory University School of Medicine, 1510 Clifton Road, Atlanta, Georgia 30322</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Géorgie (États-Unis)</region>
</placeName>
<wicri:cityArea>Department of Microbiology and Immunology and Emory Vaccine Center, Emory University School of Medicine, 1510 Clifton Road, Atlanta</wicri:cityArea>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">21288996</idno>
<idno type="pmc">3122571</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3122571</idno>
<idno type="RBID">PMC:3122571</idno>
<idno type="doi">10.1128/CVI.00435-10</idno>
<date when="2011">2011</date>
<idno type="wicri:Area/Pmc/Corpus">000675</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000675</idno>
<idno type="wicri:Area/Pmc/Curation">000675</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000675</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Microneedle Vaccination with Stabilized Recombinant Influenza Virus Hemagglutinin Induces Improved Protective Immunity
<xref ref-type="fn" rid="FN1"><sup>▿</sup>
</xref>
</title>
<author><name sortKey="Weldon, William C" sort="Weldon, William C" uniqKey="Weldon W" first="William C." last="Weldon">William C. Weldon</name>
<affiliation wicri:level="2"><nlm:aff id="aff1">Department of Microbiology and Immunology and Emory Vaccine Center, Emory University School of Medicine, 1510 Clifton Road, Atlanta, Georgia 30322</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Géorgie (États-Unis)</region>
</placeName>
<wicri:cityArea>Department of Microbiology and Immunology and Emory Vaccine Center, Emory University School of Medicine, 1510 Clifton Road, Atlanta</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Martin, Maria P" sort="Martin, Maria P" uniqKey="Martin M" first="Maria P." last="Martin">Maria P. Martin</name>
<affiliation wicri:level="2"><nlm:aff id="aff1">Department of Microbiology and Immunology and Emory Vaccine Center, Emory University School of Medicine, 1510 Clifton Road, Atlanta, Georgia 30322</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Géorgie (États-Unis)</region>
</placeName>
<wicri:cityArea>Department of Microbiology and Immunology and Emory Vaccine Center, Emory University School of Medicine, 1510 Clifton Road, Atlanta</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Zarnitsyn, Vladimir" sort="Zarnitsyn, Vladimir" uniqKey="Zarnitsyn V" first="Vladimir" last="Zarnitsyn">Vladimir Zarnitsyn</name>
<affiliation wicri:level="2"><nlm:aff id="aff2">Wallace Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, Georgia 30332</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Géorgie (États-Unis)</region>
</placeName>
<wicri:cityArea>Wallace Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Wang, Baozhong" sort="Wang, Baozhong" uniqKey="Wang B" first="Baozhong" last="Wang">Baozhong Wang</name>
<affiliation wicri:level="2"><nlm:aff id="aff1">Department of Microbiology and Immunology and Emory Vaccine Center, Emory University School of Medicine, 1510 Clifton Road, Atlanta, Georgia 30322</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Géorgie (États-Unis)</region>
</placeName>
<wicri:cityArea>Department of Microbiology and Immunology and Emory Vaccine Center, Emory University School of Medicine, 1510 Clifton Road, Atlanta</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Koutsonanos, Dimitrios" sort="Koutsonanos, Dimitrios" uniqKey="Koutsonanos D" first="Dimitrios" last="Koutsonanos">Dimitrios Koutsonanos</name>
<affiliation wicri:level="2"><nlm:aff id="aff1">Department of Microbiology and Immunology and Emory Vaccine Center, Emory University School of Medicine, 1510 Clifton Road, Atlanta, Georgia 30322</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Géorgie (États-Unis)</region>
</placeName>
<wicri:cityArea>Department of Microbiology and Immunology and Emory Vaccine Center, Emory University School of Medicine, 1510 Clifton Road, Atlanta</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Skountzou, Ioanna" sort="Skountzou, Ioanna" uniqKey="Skountzou I" first="Ioanna" last="Skountzou">Ioanna Skountzou</name>
<affiliation wicri:level="2"><nlm:aff id="aff1">Department of Microbiology and Immunology and Emory Vaccine Center, Emory University School of Medicine, 1510 Clifton Road, Atlanta, Georgia 30322</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Géorgie (États-Unis)</region>
</placeName>
<wicri:cityArea>Department of Microbiology and Immunology and Emory Vaccine Center, Emory University School of Medicine, 1510 Clifton Road, Atlanta</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Prausnitz, Mark R" sort="Prausnitz, Mark R" uniqKey="Prausnitz M" first="Mark R." last="Prausnitz">Mark R. Prausnitz</name>
<affiliation wicri:level="2"><nlm:aff id="aff2">Wallace Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, Georgia 30332</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Géorgie (États-Unis)</region>
</placeName>
<wicri:cityArea>Wallace Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Compans, Richard W" sort="Compans, Richard W" uniqKey="Compans R" first="Richard W." last="Compans">Richard W. Compans</name>
<affiliation wicri:level="2"><nlm:aff id="aff1">Department of Microbiology and Immunology and Emory Vaccine Center, Emory University School of Medicine, 1510 Clifton Road, Atlanta, Georgia 30322</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Géorgie (États-Unis)</region>
</placeName>
<wicri:cityArea>Department of Microbiology and Immunology and Emory Vaccine Center, Emory University School of Medicine, 1510 Clifton Road, Atlanta</wicri:cityArea>
</affiliation>
</author>
</analytic>
<series><title level="j">Clinical and Vaccine Immunology : CVI</title>
<idno type="ISSN">1556-6811</idno>
<idno type="eISSN">1556-679X</idno>
<imprint><date when="2011">2011</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><p>The emergence of the swine-origin 2009 influenza pandemic illustrates the need for improved vaccine production and delivery strategies. Skin-based immunization represents an attractive alternative to traditional hypodermic needle vaccination routes. Microneedles (MNs) can deliver vaccine to the epidermis and dermis, which are rich in antigen-presenting cells (APC) such as Langerhans cells and dermal dendritic cells. Previous studies using coated or dissolvable microneedles emphasized the use of inactivated influenza virus or virus-like particles as skin-based vaccines. However, most currently available influenza vaccines consist of solubilized viral protein antigens. Here we test the hypothesis that a recombinant subunit influenza vaccine can be delivered to the skin by coated microneedles and can induce protective immunity. We found that mice vaccinated via MN delivery with a stabilized recombinant trimeric soluble hemagglutinin (sHA) derived from A/Aichi/2/68 (H3) virus had significantly higher immune responses than did mice vaccinated with unmodified sHA. These mice were fully protected against a lethal challenge with influenza virus. Analysis of postchallenge lung titers showed that MN-immunized mice had completely cleared the virus from their lungs, in contrast to mice given the same vaccine by a standard subcutaneous route. In addition, we observed a higher ratio of antigen-specific Th1 cells in trimeric sHA-vaccinated mice and a greater mucosal antibody response. Our data therefore demonstrate the improved efficacy of a skin-based recombinant subunit influenza vaccine and emphasize the advantage of this route of vaccination for a protein subunit vaccine.</p>
</div>
</front>
</TEI>
<pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Clin Vaccine Immunol</journal-id>
<journal-id journal-id-type="hwp">cdli</journal-id>
<journal-id journal-id-type="pmc">cvi</journal-id>
<journal-id journal-id-type="publisher-id">CVI</journal-id>
<journal-title-group><journal-title>Clinical and Vaccine Immunology : CVI</journal-title>
</journal-title-group>
<issn pub-type="ppub">1556-6811</issn>
<issn pub-type="epub">1556-679X</issn>
<publisher><publisher-name>American Society for Microbiology</publisher-name>
<publisher-loc>1752 N St., N.W., Washington, DC</publisher-loc>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">21288996</article-id>
<article-id pub-id-type="pmc">3122571</article-id>
<article-id pub-id-type="publisher-id">0435-10</article-id>
<article-id pub-id-type="doi">10.1128/CVI.00435-10</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Vaccine Research</subject>
</subj-group>
</article-categories>
<title-group><article-title>Microneedle Vaccination with Stabilized Recombinant Influenza Virus Hemagglutinin Induces Improved Protective Immunity
<xref ref-type="fn" rid="FN1"><sup>▿</sup>
</xref>
</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Weldon</surname>
<given-names>William C.</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>1</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Martin</surname>
<given-names>Maria P.</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>1</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Zarnitsyn</surname>
<given-names>Vladimir</given-names>
</name>
<xref ref-type="aff" rid="aff2"><sup>2</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Wang</surname>
<given-names>Baozhong</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>1</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Koutsonanos</surname>
<given-names>Dimitrios</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>1</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Skountzou</surname>
<given-names>Ioanna</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>1</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Prausnitz</surname>
<given-names>Mark R.</given-names>
</name>
<xref ref-type="aff" rid="aff2"><sup>2</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Compans</surname>
<given-names>Richard W.</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>1</sup>
</xref>
<xref ref-type="corresp" rid="cor1">*</xref>
</contrib>
<aff id="aff1"><label>1</label>
Department of Microbiology and Immunology and Emory Vaccine Center, Emory University School of Medicine, 1510 Clifton Road, Atlanta, Georgia 30322</aff>
<aff id="aff2"><label>2</label>
Wallace Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, Georgia 30332</aff>
</contrib-group>
<author-notes><corresp id="cor1"><label>*</label>
Corresponding author. Mailing address: <addr-line>Department of Microbiology and Immunology and Emory Vaccine Center, Emory University School of Medicine, 1510 Clifton Road, Atlanta, GA 30322</addr-line>
. Phone: <phone>(404) 727-3531</phone>
. Fax: <fax>(404) 727-3295</fax>
. E-mail: <email>rcompan@emory.edu</email>
.</corresp>
</author-notes>
<pub-date pub-type="ppub"><month>4</month>
<year>2011</year>
</pub-date>
<volume>18</volume>
<issue>4</issue>
<fpage>647</fpage>
<lpage>654</lpage>
<history><date date-type="received"><day>7</day>
<month>10</month>
<year>2010</year>
</date>
<date date-type="rev-request"><day>5</day>
<month>1</month>
<year>2011</year>
</date>
<date date-type="accepted"><day>23</day>
<month>1</month>
<year>2011</year>
</date>
</history>
<permissions><copyright-statement>Copyright © 2011, American Society for Microbiology</copyright-statement>
<copyright-year>2011</copyright-year>
<copyright-holder>American Society for Microbiology</copyright-holder>
</permissions>
<self-uri xlink:title="pdf" xlink:type="simple" xlink:href="zcd00411000647.pdf"></self-uri>
<abstract><p>The emergence of the swine-origin 2009 influenza pandemic illustrates the need for improved vaccine production and delivery strategies. Skin-based immunization represents an attractive alternative to traditional hypodermic needle vaccination routes. Microneedles (MNs) can deliver vaccine to the epidermis and dermis, which are rich in antigen-presenting cells (APC) such as Langerhans cells and dermal dendritic cells. Previous studies using coated or dissolvable microneedles emphasized the use of inactivated influenza virus or virus-like particles as skin-based vaccines. However, most currently available influenza vaccines consist of solubilized viral protein antigens. Here we test the hypothesis that a recombinant subunit influenza vaccine can be delivered to the skin by coated microneedles and can induce protective immunity. We found that mice vaccinated via MN delivery with a stabilized recombinant trimeric soluble hemagglutinin (sHA) derived from A/Aichi/2/68 (H3) virus had significantly higher immune responses than did mice vaccinated with unmodified sHA. These mice were fully protected against a lethal challenge with influenza virus. Analysis of postchallenge lung titers showed that MN-immunized mice had completely cleared the virus from their lungs, in contrast to mice given the same vaccine by a standard subcutaneous route. In addition, we observed a higher ratio of antigen-specific Th1 cells in trimeric sHA-vaccinated mice and a greater mucosal antibody response. Our data therefore demonstrate the improved efficacy of a skin-based recombinant subunit influenza vaccine and emphasize the advantage of this route of vaccination for a protein subunit vaccine.</p>
</abstract>
</article-meta>
</front>
</pmc>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/PandemieGrippaleV1/Data/Pmc/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000675 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Pmc/Curation/biblio.hfd -nk 000675 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= PandemieGrippaleV1 |flux= Pmc |étape= Curation |type= RBID |clé= PMC:3122571 |texte= Microneedle Vaccination with Stabilized Recombinant Influenza Virus Hemagglutinin Induces Improved Protective Immunity ▿ }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Pmc/Curation/RBID.i -Sk "pubmed:21288996" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Pmc/Curation/biblio.hfd \ | NlmPubMed2Wicri -a PandemieGrippaleV1
![]() | This area was generated with Dilib version V0.6.34. | ![]() |