Expression of hemagglutinin protein from the avian influenza virus H5N1 in a baculovirus/insect cell system significantly enhanced by suspension culture
Identifieur interne : 000E55 ( Pmc/Checkpoint ); précédent : 000E54; suivant : 000E56Expression of hemagglutinin protein from the avian influenza virus H5N1 in a baculovirus/insect cell system significantly enhanced by suspension culture
Auteurs : Nitar Nwe [Singapour] ; Qigai He [Singapour] ; Sudarat Damrongwatanapokin [Thaïlande] ; Qingyun Du [Singapour] ; Ivanus Manopo [Singapour] ; Yukol Limlamthong [Thaïlande] ; Beau James Fenner [Singapour] ; Lynn Spencer [Singapour] ; Jimmy Kwang [Singapour]Source :
- BMC Microbiology [ 1471-2180 ] ; 2006.
Abstract
Prevention of a possible avian influenza pandemic necessitates the development of rapid diagnostic tests and the eventual production of a vaccine.
For vaccine production, hemagglutinin (HA1) from avian influenza H5N1 was expressed from a recombinant baculovirus. Recombinant HA1 was expressed in monolayer or suspension culture insect cells by infection with the recombinant baculovirus. The yield of rHA1 from the suspension culture was 68 mg/l, compared to 6 mg/l from the monolayer culture. Immunization of guinea pigs with 50 μg of rHA1 yielded hemagglutinin inhibition and virus neutralization titers of 1:160 after two times vaccination with rHA1 protein.
Thus, the production of rHA1 using an insect suspension cell system provides a promising basis for economical production of a H5 antigen.
Url:
DOI: 10.1186/1471-2180-6-16
PubMed: 16504108
PubMed Central: 1397843
Affiliations:
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<front><div type="abstract" xml:lang="en"><sec><title>Background</title>
<p>Prevention of a possible avian influenza pandemic necessitates the development of rapid diagnostic tests and the eventual production of a vaccine.</p>
</sec>
<sec><title>Results</title>
<p>For vaccine production, hemagglutinin (HA1) from avian influenza H5N1 was expressed from a recombinant baculovirus. Recombinant HA1 was expressed in monolayer or suspension culture insect cells by infection with the recombinant baculovirus. The yield of rHA1 from the suspension culture was 68 mg/l, compared to 6 mg/l from the monolayer culture. Immunization of guinea pigs with 50 μg of rHA1 yielded hemagglutinin inhibition and virus neutralization titers of 1:160 after two times vaccination with rHA1 protein.</p>
</sec>
<sec><title>Conclusion</title>
<p>Thus, the production of rHA1 using an insect suspension cell system provides a promising basis for economical production of a H5 antigen.</p>
</sec>
</div>
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<front><journal-meta><journal-id journal-id-type="nlm-ta">BMC Microbiol</journal-id>
<journal-title>BMC Microbiology</journal-title>
<issn pub-type="epub">1471-2180</issn>
<publisher><publisher-name>BioMed Central</publisher-name>
<publisher-loc>London</publisher-loc>
</publisher>
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<article-meta><article-id pub-id-type="pmid">16504108</article-id>
<article-id pub-id-type="pmc">1397843</article-id>
<article-id pub-id-type="publisher-id">1471-2180-6-16</article-id>
<article-id pub-id-type="doi">10.1186/1471-2180-6-16</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject>
</subj-group>
</article-categories>
<title-group><article-title>Expression of hemagglutinin protein from the avian influenza virus H5N1 in a baculovirus/insect cell system significantly enhanced by suspension culture</article-title>
</title-group>
<contrib-group><contrib id="A1" contrib-type="author"><name><surname>Nwe</surname>
<given-names>Nitar</given-names>
</name>
<xref ref-type="aff" rid="I1">1</xref>
<email>nitarnwe@yahoo.com</email>
</contrib>
<contrib id="A2" contrib-type="author"><name><surname>He</surname>
<given-names>Qigai</given-names>
</name>
<xref ref-type="aff" rid="I1">1</xref>
<email>qigai@tll.org.sg</email>
</contrib>
<contrib id="A3" contrib-type="author"><name><surname>Damrongwatanapokin</surname>
<given-names>Sudarat</given-names>
</name>
<xref ref-type="aff" rid="I2">2</xref>
<email>d-sudarat@hotmail.com</email>
</contrib>
<contrib id="A4" contrib-type="author"><name><surname>Du</surname>
<given-names>Qingyun</given-names>
</name>
<xref ref-type="aff" rid="I1">1</xref>
<email>qingyun@tll.org.sg</email>
</contrib>
<contrib id="A5" contrib-type="author"><name><surname>Manopo</surname>
<given-names>Ivanus</given-names>
</name>
<xref ref-type="aff" rid="I1">1</xref>
<email>ivanus@tll.org.sg</email>
</contrib>
<contrib id="A6" contrib-type="author"><name><surname>Limlamthong</surname>
<given-names>Yukol</given-names>
</name>
<xref ref-type="aff" rid="I2">2</xref>
<email>dg@dld.go.th</email>
</contrib>
<contrib id="A7" contrib-type="author"><name><surname>Fenner</surname>
<given-names>Beau James</given-names>
</name>
<xref ref-type="aff" rid="I1">1</xref>
<email>beau@tll.org.sg</email>
</contrib>
<contrib id="A8" contrib-type="author"><name><surname>Spencer</surname>
<given-names>Lynn</given-names>
</name>
<xref ref-type="aff" rid="I1">1</xref>
<email>lynn@tll.org.sg</email>
</contrib>
<contrib id="A9" corresp="yes" contrib-type="author"><name><surname>Kwang</surname>
<given-names>Jimmy</given-names>
</name>
<xref ref-type="aff" rid="I1">1</xref>
<email>kwang@tll.org.sg</email>
</contrib>
</contrib-group>
<aff id="I1"><label>1</label>
Animal Health Biotechnology Group, Temasek Life Science Laboratory, 1 Research Link, National University of Singapore, Singapore 117604, Singapore</aff>
<aff id="I2"><label>2</label>
Department of Livestock Development, National Institute of Animal Health, Chatuchak, Bangkok, Thailand</aff>
<pub-date pub-type="collection"><year>2006</year>
</pub-date>
<pub-date pub-type="epub"><day>24</day>
<month>2</month>
<year>2006</year>
</pub-date>
<volume>6</volume>
<fpage>16</fpage>
<lpage>16</lpage>
<ext-link ext-link-type="uri" xlink:href="http://www.biomedcentral.com/1471-2180/6/16"></ext-link>
<history><date date-type="received"><day>19</day>
<month>8</month>
<year>2005</year>
</date>
<date date-type="accepted"><day>24</day>
<month>2</month>
<year>2006</year>
</date>
</history>
<copyright-statement>Copyright © 2006 Nwe et al; licensee BioMed Central Ltd.</copyright-statement>
<license license-type="open-access" xlink:href="http://creativecommons.org/licenses/by/2.0"><p>This is an Open Access article distributed under the terms of the Creative Commons Attribution License (<ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by/2.0"></ext-link>
), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</p>
</license>
<abstract><sec><title>Background</title>
<p>Prevention of a possible avian influenza pandemic necessitates the development of rapid diagnostic tests and the eventual production of a vaccine.</p>
</sec>
<sec><title>Results</title>
<p>For vaccine production, hemagglutinin (HA1) from avian influenza H5N1 was expressed from a recombinant baculovirus. Recombinant HA1 was expressed in monolayer or suspension culture insect cells by infection with the recombinant baculovirus. The yield of rHA1 from the suspension culture was 68 mg/l, compared to 6 mg/l from the monolayer culture. Immunization of guinea pigs with 50 μg of rHA1 yielded hemagglutinin inhibition and virus neutralization titers of 1:160 after two times vaccination with rHA1 protein.</p>
</sec>
<sec><title>Conclusion</title>
<p>Thus, the production of rHA1 using an insect suspension cell system provides a promising basis for economical production of a H5 antigen.</p>
</sec>
</abstract>
</article-meta>
</front>
</pmc>
<affiliations><list><country><li>Singapour</li>
<li>Thaïlande</li>
</country>
<orgName><li>Université nationale de Singapour</li>
</orgName>
</list>
<tree><country name="Singapour"><noRegion><name sortKey="Nwe, Nitar" sort="Nwe, Nitar" uniqKey="Nwe N" first="Nitar" last="Nwe">Nitar Nwe</name>
</noRegion>
<name sortKey="Du, Qingyun" sort="Du, Qingyun" uniqKey="Du Q" first="Qingyun" last="Du">Qingyun Du</name>
<name sortKey="Fenner, Beau James" sort="Fenner, Beau James" uniqKey="Fenner B" first="Beau James" last="Fenner">Beau James Fenner</name>
<name sortKey="He, Qigai" sort="He, Qigai" uniqKey="He Q" first="Qigai" last="He">Qigai He</name>
<name sortKey="Kwang, Jimmy" sort="Kwang, Jimmy" uniqKey="Kwang J" first="Jimmy" last="Kwang">Jimmy Kwang</name>
<name sortKey="Manopo, Ivanus" sort="Manopo, Ivanus" uniqKey="Manopo I" first="Ivanus" last="Manopo">Ivanus Manopo</name>
<name sortKey="Spencer, Lynn" sort="Spencer, Lynn" uniqKey="Spencer L" first="Lynn" last="Spencer">Lynn Spencer</name>
</country>
<country name="Thaïlande"><noRegion><name sortKey="Damrongwatanapokin, Sudarat" sort="Damrongwatanapokin, Sudarat" uniqKey="Damrongwatanapokin S" first="Sudarat" last="Damrongwatanapokin">Sudarat Damrongwatanapokin</name>
</noRegion>
<name sortKey="Limlamthong, Yukol" sort="Limlamthong, Yukol" uniqKey="Limlamthong Y" first="Yukol" last="Limlamthong">Yukol Limlamthong</name>
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