Expression of hemagglutinin protein from the avian influenza virus H5N1 in a baculovirus/insect cell system significantly enhanced by suspension culture
Identifieur interne : 000E55 ( Pmc/Checkpoint ); précédent : 000E54; suivant : 000E56Expression of hemagglutinin protein from the avian influenza virus H5N1 in a baculovirus/insect cell system significantly enhanced by suspension culture
Auteurs : Nitar Nwe [Singapour] ; Qigai He [Singapour] ; Sudarat Damrongwatanapokin [Thaïlande] ; Qingyun Du [Singapour] ; Ivanus Manopo [Singapour] ; Yukol Limlamthong [Thaïlande] ; Beau James Fenner [Singapour] ; Lynn Spencer [Singapour] ; Jimmy Kwang [Singapour]Source :
- BMC Microbiology [ 1471-2180 ] ; 2006.
Abstract
Prevention of a possible avian influenza pandemic necessitates the development of rapid diagnostic tests and the eventual production of a vaccine.
For vaccine production, hemagglutinin (HA1) from avian influenza H5N1 was expressed from a recombinant baculovirus. Recombinant HA1 was expressed in monolayer or suspension culture insect cells by infection with the recombinant baculovirus. The yield of rHA1 from the suspension culture was 68 mg/l, compared to 6 mg/l from the monolayer culture. Immunization of guinea pigs with 50 μg of rHA1 yielded hemagglutinin inhibition and virus neutralization titers of 1:160 after two times vaccination with rHA1 protein.
Thus, the production of rHA1 using an insect suspension cell system provides a promising basis for economical production of a H5 antigen.
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DOI: 10.1186/1471-2180-6-16
PubMed: 16504108
PubMed Central: 1397843
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id="I1">Animal Health Biotechnology Group, Temasek Life Science Laboratory, 1 Research Link, National University of Singapore, Singapore 117604, Singapore</nlm:aff><country xml:lang="fr">Singapour</country><wicri:regionArea>Animal Health Biotechnology Group, Temasek Life Science Laboratory, 1 Research Link, National University of Singapore, Singapore 117604</wicri:regionArea><orgName type="university">Université nationale de Singapour</orgName></affiliation></author><author><name sortKey="He, Qigai" sort="He, Qigai" uniqKey="He Q" first="Qigai" last="He">Qigai He</name><affiliation wicri:level="4"><nlm:aff id="I1">Animal Health Biotechnology Group, Temasek Life Science Laboratory, 1 Research Link, National University of Singapore, Singapore 117604, Singapore</nlm:aff><country xml:lang="fr">Singapour</country><wicri:regionArea>Animal Health Biotechnology Group, Temasek Life Science Laboratory, 1 Research Link, National University of Singapore, Singapore 117604</wicri:regionArea><orgName type="university">Université nationale de Singapour</orgName></affiliation></author><author><name sortKey="Damrongwatanapokin, Sudarat" sort="Damrongwatanapokin, Sudarat" uniqKey="Damrongwatanapokin S" first="Sudarat" last="Damrongwatanapokin">Sudarat Damrongwatanapokin</name><affiliation wicri:level="1"><nlm:aff id="I2">Department of Livestock Development, National Institute of Animal Health, Chatuchak, Bangkok, Thailand</nlm:aff><country xml:lang="fr">Thaïlande</country><wicri:regionArea>Department of Livestock Development, National Institute of Animal Health, Chatuchak, Bangkok</wicri:regionArea><wicri:noRegion>Bangkok</wicri:noRegion></affiliation></author><author><name sortKey="Du, Qingyun" sort="Du, Qingyun" uniqKey="Du Q" first="Qingyun" last="Du">Qingyun Du</name><affiliation wicri:level="4"><nlm:aff id="I1">Animal Health Biotechnology Group, Temasek Life Science Laboratory, 1 Research Link, National University of Singapore, Singapore 117604, Singapore</nlm:aff><country xml:lang="fr">Singapour</country><wicri:regionArea>Animal Health Biotechnology Group, Temasek Life Science Laboratory, 1 Research Link, National University of Singapore, Singapore 117604</wicri:regionArea><orgName type="university">Université nationale de Singapour</orgName></affiliation></author><author><name sortKey="Manopo, Ivanus" sort="Manopo, Ivanus" uniqKey="Manopo I" first="Ivanus" last="Manopo">Ivanus Manopo</name><affiliation wicri:level="4"><nlm:aff id="I1">Animal Health Biotechnology Group, Temasek Life Science Laboratory, 1 Research Link, National University of Singapore, Singapore 117604, Singapore</nlm:aff><country xml:lang="fr">Singapour</country><wicri:regionArea>Animal Health Biotechnology Group, Temasek Life Science Laboratory, 1 Research Link, National University of Singapore, Singapore 117604</wicri:regionArea><orgName type="university">Université nationale de Singapour</orgName></affiliation></author><author><name sortKey="Limlamthong, Yukol" sort="Limlamthong, Yukol" uniqKey="Limlamthong Y" first="Yukol" last="Limlamthong">Yukol Limlamthong</name><affiliation wicri:level="1"><nlm:aff id="I2">Department of Livestock Development, National Institute of Animal Health, Chatuchak, Bangkok, Thailand</nlm:aff><country xml:lang="fr">Thaïlande</country><wicri:regionArea>Department of Livestock Development, National Institute of Animal Health, Chatuchak, Bangkok</wicri:regionArea><wicri:noRegion>Bangkok</wicri:noRegion></affiliation></author><author><name sortKey="Fenner, Beau James" sort="Fenner, Beau James" uniqKey="Fenner B" first="Beau James" last="Fenner">Beau James Fenner</name><affiliation wicri:level="4"><nlm:aff id="I1">Animal Health Biotechnology Group, Temasek Life Science Laboratory, 1 Research Link, National University of Singapore, Singapore 117604, Singapore</nlm:aff><country xml:lang="fr">Singapour</country><wicri:regionArea>Animal Health Biotechnology Group, Temasek Life Science Laboratory, 1 Research Link, National University of Singapore, Singapore 117604</wicri:regionArea><orgName type="university">Université nationale de Singapour</orgName></affiliation></author><author><name sortKey="Spencer, Lynn" sort="Spencer, Lynn" uniqKey="Spencer L" first="Lynn" last="Spencer">Lynn Spencer</name><affiliation wicri:level="4"><nlm:aff id="I1">Animal Health Biotechnology Group, Temasek Life Science Laboratory, 1 Research Link, National University of Singapore, Singapore 117604, Singapore</nlm:aff><country xml:lang="fr">Singapour</country><wicri:regionArea>Animal Health Biotechnology Group, Temasek Life Science Laboratory, 1 Research Link, National University of Singapore, Singapore 117604</wicri:regionArea><orgName type="university">Université nationale de Singapour</orgName></affiliation></author><author><name sortKey="Kwang, Jimmy" sort="Kwang, Jimmy" uniqKey="Kwang J" first="Jimmy" last="Kwang">Jimmy Kwang</name><affiliation wicri:level="4"><nlm:aff id="I1">Animal Health Biotechnology Group, Temasek Life Science Laboratory, 1 Research Link, National University of Singapore, Singapore 117604, Singapore</nlm:aff><country xml:lang="fr">Singapour</country><wicri:regionArea>Animal Health Biotechnology Group, Temasek Life Science Laboratory, 1 Research Link, National University of Singapore, Singapore 117604</wicri:regionArea><orgName type="university">Université nationale de Singapour</orgName></affiliation></author></analytic><series><title level="j">BMC Microbiology</title><idno type="eISSN">1471-2180</idno><imprint><date when="2006">2006</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec><title>Background</title><p>Prevention of a possible avian influenza pandemic necessitates the development of rapid diagnostic tests and the eventual production of a vaccine.</p></sec><sec><title>Results</title><p>For vaccine production, hemagglutinin (HA1) from avian influenza H5N1 was expressed from a recombinant baculovirus. Recombinant HA1 was expressed in monolayer or suspension culture insect cells by infection with the recombinant baculovirus. The yield of rHA1 from the suspension culture was 68 mg/l, compared to 6 mg/l from the monolayer culture. Immunization of guinea pigs with 50 μg of rHA1 yielded hemagglutinin inhibition and virus neutralization titers of 1:160 after two times vaccination with rHA1 protein.</p></sec><sec><title>Conclusion</title><p>Thus, the production of rHA1 using an insect suspension cell system provides a promising basis for economical production of a H5 antigen.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct></listBibl></div1></back></TEI><pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">BMC Microbiol</journal-id><journal-title>BMC Microbiology</journal-title><issn pub-type="epub">1471-2180</issn><publisher><publisher-name>BioMed Central</publisher-name><publisher-loc>London</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">16504108</article-id><article-id pub-id-type="pmc">1397843</article-id><article-id pub-id-type="publisher-id">1471-2180-6-16</article-id><article-id pub-id-type="doi">10.1186/1471-2180-6-16</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group></article-categories><title-group><article-title>Expression of hemagglutinin protein from the avian influenza virus H5N1 in a baculovirus/insect cell system significantly enhanced by suspension culture</article-title></title-group><contrib-group><contrib id="A1" contrib-type="author"><name><surname>Nwe</surname><given-names>Nitar</given-names></name><xref ref-type="aff" rid="I1">1</xref><email>nitarnwe@yahoo.com</email></contrib><contrib id="A2" contrib-type="author"><name><surname>He</surname><given-names>Qigai</given-names></name><xref ref-type="aff" rid="I1">1</xref><email>qigai@tll.org.sg</email></contrib><contrib id="A3" contrib-type="author"><name><surname>Damrongwatanapokin</surname><given-names>Sudarat</given-names></name><xref ref-type="aff" rid="I2">2</xref><email>d-sudarat@hotmail.com</email></contrib><contrib id="A4" contrib-type="author"><name><surname>Du</surname><given-names>Qingyun</given-names></name><xref ref-type="aff" rid="I1">1</xref><email>qingyun@tll.org.sg</email></contrib><contrib id="A5" contrib-type="author"><name><surname>Manopo</surname><given-names>Ivanus</given-names></name><xref ref-type="aff" rid="I1">1</xref><email>ivanus@tll.org.sg</email></contrib><contrib id="A6" contrib-type="author"><name><surname>Limlamthong</surname><given-names>Yukol</given-names></name><xref ref-type="aff" rid="I2">2</xref><email>dg@dld.go.th</email></contrib><contrib id="A7" contrib-type="author"><name><surname>Fenner</surname><given-names>Beau James</given-names></name><xref ref-type="aff" rid="I1">1</xref><email>beau@tll.org.sg</email></contrib><contrib id="A8" contrib-type="author"><name><surname>Spencer</surname><given-names>Lynn</given-names></name><xref ref-type="aff" rid="I1">1</xref><email>lynn@tll.org.sg</email></contrib><contrib id="A9" corresp="yes" contrib-type="author"><name><surname>Kwang</surname><given-names>Jimmy</given-names></name><xref ref-type="aff" rid="I1">1</xref><email>kwang@tll.org.sg</email></contrib></contrib-group><aff id="I1"><label>1</label>Animal Health Biotechnology Group, Temasek Life Science Laboratory, 1 Research Link, National University of Singapore, Singapore 117604, Singapore</aff><aff id="I2"><label>2</label>Department of Livestock Development, National Institute of Animal Health, Chatuchak, Bangkok, Thailand</aff><pub-date pub-type="collection"><year>2006</year></pub-date><pub-date pub-type="epub"><day>24</day><month>2</month><year>2006</year></pub-date><volume>6</volume><fpage>16</fpage><lpage>16</lpage><ext-link ext-link-type="uri" xlink:href="http://www.biomedcentral.com/1471-2180/6/16"></ext-link><history><date date-type="received"><day>19</day><month>8</month><year>2005</year></date><date date-type="accepted"><day>24</day><month>2</month><year>2006</year></date></history><copyright-statement>Copyright © 2006 Nwe et al; licensee BioMed Central Ltd.</copyright-statement><license license-type="open-access" xlink:href="http://creativecommons.org/licenses/by/2.0"><p>This is an Open Access article distributed under the terms of the Creative Commons Attribution License (<ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by/2.0"></ext-link>), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</p></license><abstract><sec><title>Background</title><p>Prevention of a possible avian influenza pandemic necessitates the development of rapid diagnostic tests and the eventual production of a vaccine.</p></sec><sec><title>Results</title><p>For vaccine production, hemagglutinin (HA1) from avian influenza H5N1 was expressed from a recombinant baculovirus. Recombinant HA1 was expressed in monolayer or suspension culture insect cells by infection with the recombinant baculovirus. The yield of rHA1 from the suspension culture was 68 mg/l, compared to 6 mg/l from the monolayer culture. Immunization of guinea pigs with 50 μg of rHA1 yielded hemagglutinin inhibition and virus neutralization titers of 1:160 after two times vaccination with rHA1 protein.</p></sec><sec><title>Conclusion</title><p>Thus, the production of rHA1 using an insect suspension cell system provides a promising basis for economical production of a H5 antigen.</p></sec></abstract></article-meta></front></pmc><affiliations><list><country><li>Singapour</li><li>Thaïlande</li></country><orgName><li>Université nationale de Singapour</li></orgName></list><tree><country name="Singapour"><noRegion><name sortKey="Nwe, Nitar" sort="Nwe, Nitar" uniqKey="Nwe N" first="Nitar" last="Nwe">Nitar Nwe</name></noRegion><name sortKey="Du, Qingyun" sort="Du, Qingyun" uniqKey="Du Q" first="Qingyun" last="Du">Qingyun Du</name><name sortKey="Fenner, Beau James" sort="Fenner, Beau James" uniqKey="Fenner B" first="Beau James" last="Fenner">Beau James Fenner</name><name sortKey="He, Qigai" sort="He, Qigai" uniqKey="He Q" first="Qigai" last="He">Qigai He</name><name sortKey="Kwang, Jimmy" sort="Kwang, Jimmy" uniqKey="Kwang J" first="Jimmy" last="Kwang">Jimmy Kwang</name><name sortKey="Manopo, Ivanus" sort="Manopo, Ivanus" uniqKey="Manopo I" first="Ivanus" last="Manopo">Ivanus Manopo</name><name sortKey="Spencer, Lynn" sort="Spencer, Lynn" uniqKey="Spencer L" first="Lynn" last="Spencer">Lynn Spencer</name></country><country name="Thaïlande"><noRegion><name sortKey="Damrongwatanapokin, Sudarat" sort="Damrongwatanapokin, Sudarat" uniqKey="Damrongwatanapokin S" first="Sudarat" last="Damrongwatanapokin">Sudarat Damrongwatanapokin</name></noRegion><name sortKey="Limlamthong, Yukol" sort="Limlamthong, Yukol" uniqKey="Limlamthong Y" first="Yukol" last="Limlamthong">Yukol Limlamthong</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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