Plasmacytoid Dendritic Cells and CD8 T Cells From Pregnant Women Show Altered Phenotype and Function Following H1N1/09 Infection
Identifieur interne : 000221 ( PascalFrancis/Corpus ); précédent : 000220; suivant : 000222Plasmacytoid Dendritic Cells and CD8 T Cells From Pregnant Women Show Altered Phenotype and Function Following H1N1/09 Infection
Auteurs : Rebecca L. Vanders ; Peter G. Gibson ; Vanessa E. Murphy ; Peter A. B. WarkSource :
- The Journal of infectious diseases [ 0022-1899 ] ; 2013.
Descripteurs français
- Pascal (Inist)
English descriptors
- KwdEn :
Abstract
Background. Pregnant women are a high-risk group during influenza pandemics. In this study we determined whether plasmacytoid dendritic cells (pDCs) and CD8 T cells from pregnant women display altered activity following in vitro infection with 2009 pandemic swine influenza (H1N1/09). Methods. Peripheral blood mononuclear cells (PBMCs) were isolated from pregnant (n = 26) and nonpregnant (n = 28) women. DC subtypes were enumerated from PBMCs. PBMCs were infected with H1N1/09 and CD86, human leukocyte antigen-DR (HLA-DR), and programmed death ligand 1/2 (PDL1/2) measured on pDCs. PD receptor 1 (PD1) was measured on CD8 T cells. Interferon-alpha (IFN-α), interleukin-2 (IL-2), tumor necrosis factor-alpha (TNFα), and IFN-gamma were measured from culture supernatant. Results. pDC (ie, CD303+/CD1c- PBMCs) percentages were lower in pregnant compared with nonpregnant women (P < .05). Following H1N1/09 infection, pDCs from pregnant women showed higher expression of CD86 (P < .01), HLA-DR (P < .001), and PDL1 (P < .001) compared with nonpregnant women. Expression of PD1 on CD8 T cells was higher during pregnancy (P < .05). Following H1N1/09 infection, PBMCs from pregnant women displayed reduced IFN-a (P < .01), IL-2 (P < .01), and IFN-γ (P < .01) compared with nonpregnant women. Blocking PDL1 during H1N1/09 infection increased these cytokines during pregnancy (P < .05). Conclusion. Altered maternal cellular antiviral activity is implicated in the increased morbidity during pregnancy following influenza pandemics.
Notice en format standard (ISO 2709)
Pour connaître la documentation sur le format Inist Standard.
pA |
|
---|
Format Inist (serveur)
NO : | PASCAL 13-0310021 INIST |
---|---|
ET : | Plasmacytoid Dendritic Cells and CD8 T Cells From Pregnant Women Show Altered Phenotype and Function Following H1N1/09 Infection |
AU : | VANDERS (Rebecca L.); GIBSON (Peter G.); MURPHY (Vanessa E.); WARK (Peter A. B.) |
AF : | Centre for Asthma and Respiratory Diseases, School of Medicine and Public Health, The University of Newcastle/Australie (1 aut., 2 aut., 3 aut., 4 aut.); Hunter Medical Research Institute, John Hunter Hospital/Newcastle/Australie (1 aut., 3 aut.); Department of Respiratory and Sleep Medicine, John Hunter Hospital/Newcastle/Australie (2 aut., 4 aut.) |
DT : | Publication en série; Niveau analytique |
SO : | The Journal of infectious diseases; ISSN 0022-1899; Coden JIDIAQ; Royaume-Uni; Da. 2013; Vol. 208; No. 7; Pp. 1062-1070; Bibl. 42 ref. |
LA : | Anglais |
EA : | Background. Pregnant women are a high-risk group during influenza pandemics. In this study we determined whether plasmacytoid dendritic cells (pDCs) and CD8 T cells from pregnant women display altered activity following in vitro infection with 2009 pandemic swine influenza (H1N1/09). Methods. Peripheral blood mononuclear cells (PBMCs) were isolated from pregnant (n = 26) and nonpregnant (n = 28) women. DC subtypes were enumerated from PBMCs. PBMCs were infected with H1N1/09 and CD86, human leukocyte antigen-DR (HLA-DR), and programmed death ligand 1/2 (PDL1/2) measured on pDCs. PD receptor 1 (PD1) was measured on CD8 T cells. Interferon-alpha (IFN-α), interleukin-2 (IL-2), tumor necrosis factor-alpha (TNFα), and IFN-gamma were measured from culture supernatant. Results. pDC (ie, CD303+/CD1c- PBMCs) percentages were lower in pregnant compared with nonpregnant women (P < .05). Following H1N1/09 infection, pDCs from pregnant women showed higher expression of CD86 (P < .01), HLA-DR (P < .001), and PDL1 (P < .001) compared with nonpregnant women. Expression of PD1 on CD8 T cells was higher during pregnancy (P < .05). Following H1N1/09 infection, PBMCs from pregnant women displayed reduced IFN-a (P < .01), IL-2 (P < .01), and IFN-γ (P < .01) compared with nonpregnant women. Blocking PDL1 during H1N1/09 infection increased these cytokines during pregnancy (P < .05). Conclusion. Altered maternal cellular antiviral activity is implicated in the increased morbidity during pregnancy following influenza pandemics. |
CC : | 002A05; 002B05 |
FD : | Homme; Cellule dendritique; Lymphocyte T CD8; Gestation; Femelle; Femme; Phénotype; Infection; Virus grippal A(H1N1) |
ED : | Human; Dendritic cell; CD8 T lymphocyte; Pregnancy; Female; Woman; Phenotype; Infection; Influenzavirus A(H1N1) |
SD : | Hombre; Célula dendrítica; Linfocito T CD8; Gestación; Hembra; Mujer; Fenotipo; Infección |
LO : | INIST-2052.354000505852320040 |
ID : | 13-0310021 |
Links to Exploration step
Pascal:13-0310021Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">Plasmacytoid Dendritic Cells and CD8 T Cells From Pregnant Women Show Altered Phenotype and Function Following H1N1/09 Infection</title>
<author><name sortKey="Vanders, Rebecca L" sort="Vanders, Rebecca L" uniqKey="Vanders R" first="Rebecca L." last="Vanders">Rebecca L. Vanders</name>
<affiliation><inist:fA14 i1="01"><s1>Centre for Asthma and Respiratory Diseases, School of Medicine and Public Health, The University of Newcastle</s1>
<s3>AUS</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
</inist:fA14>
</affiliation>
<affiliation><inist:fA14 i1="02"><s1>Hunter Medical Research Institute, John Hunter Hospital</s1>
<s2>Newcastle</s2>
<s3>AUS</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Gibson, Peter G" sort="Gibson, Peter G" uniqKey="Gibson P" first="Peter G." last="Gibson">Peter G. Gibson</name>
<affiliation><inist:fA14 i1="01"><s1>Centre for Asthma and Respiratory Diseases, School of Medicine and Public Health, The University of Newcastle</s1>
<s3>AUS</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
</inist:fA14>
</affiliation>
<affiliation><inist:fA14 i1="03"><s1>Department of Respiratory and Sleep Medicine, John Hunter Hospital</s1>
<s2>Newcastle</s2>
<s3>AUS</s3>
<sZ>2 aut.</sZ>
<sZ>4 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Murphy, Vanessa E" sort="Murphy, Vanessa E" uniqKey="Murphy V" first="Vanessa E." last="Murphy">Vanessa E. Murphy</name>
<affiliation><inist:fA14 i1="01"><s1>Centre for Asthma and Respiratory Diseases, School of Medicine and Public Health, The University of Newcastle</s1>
<s3>AUS</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
</inist:fA14>
</affiliation>
<affiliation><inist:fA14 i1="02"><s1>Hunter Medical Research Institute, John Hunter Hospital</s1>
<s2>Newcastle</s2>
<s3>AUS</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Wark, Peter A B" sort="Wark, Peter A B" uniqKey="Wark P" first="Peter A. B." last="Wark">Peter A. B. Wark</name>
<affiliation><inist:fA14 i1="01"><s1>Centre for Asthma and Respiratory Diseases, School of Medicine and Public Health, The University of Newcastle</s1>
<s3>AUS</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
</inist:fA14>
</affiliation>
<affiliation><inist:fA14 i1="03"><s1>Department of Respiratory and Sleep Medicine, John Hunter Hospital</s1>
<s2>Newcastle</s2>
<s3>AUS</s3>
<sZ>2 aut.</sZ>
<sZ>4 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">INIST</idno>
<idno type="inist">13-0310021</idno>
<date when="2013">2013</date>
<idno type="stanalyst">PASCAL 13-0310021 INIST</idno>
<idno type="RBID">Pascal:13-0310021</idno>
<idno type="wicri:Area/PascalFrancis/Corpus">000221</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Plasmacytoid Dendritic Cells and CD8 T Cells From Pregnant Women Show Altered Phenotype and Function Following H1N1/09 Infection</title>
<author><name sortKey="Vanders, Rebecca L" sort="Vanders, Rebecca L" uniqKey="Vanders R" first="Rebecca L." last="Vanders">Rebecca L. Vanders</name>
<affiliation><inist:fA14 i1="01"><s1>Centre for Asthma and Respiratory Diseases, School of Medicine and Public Health, The University of Newcastle</s1>
<s3>AUS</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
</inist:fA14>
</affiliation>
<affiliation><inist:fA14 i1="02"><s1>Hunter Medical Research Institute, John Hunter Hospital</s1>
<s2>Newcastle</s2>
<s3>AUS</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Gibson, Peter G" sort="Gibson, Peter G" uniqKey="Gibson P" first="Peter G." last="Gibson">Peter G. Gibson</name>
<affiliation><inist:fA14 i1="01"><s1>Centre for Asthma and Respiratory Diseases, School of Medicine and Public Health, The University of Newcastle</s1>
<s3>AUS</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
</inist:fA14>
</affiliation>
<affiliation><inist:fA14 i1="03"><s1>Department of Respiratory and Sleep Medicine, John Hunter Hospital</s1>
<s2>Newcastle</s2>
<s3>AUS</s3>
<sZ>2 aut.</sZ>
<sZ>4 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Murphy, Vanessa E" sort="Murphy, Vanessa E" uniqKey="Murphy V" first="Vanessa E." last="Murphy">Vanessa E. Murphy</name>
<affiliation><inist:fA14 i1="01"><s1>Centre for Asthma and Respiratory Diseases, School of Medicine and Public Health, The University of Newcastle</s1>
<s3>AUS</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
</inist:fA14>
</affiliation>
<affiliation><inist:fA14 i1="02"><s1>Hunter Medical Research Institute, John Hunter Hospital</s1>
<s2>Newcastle</s2>
<s3>AUS</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Wark, Peter A B" sort="Wark, Peter A B" uniqKey="Wark P" first="Peter A. B." last="Wark">Peter A. B. Wark</name>
<affiliation><inist:fA14 i1="01"><s1>Centre for Asthma and Respiratory Diseases, School of Medicine and Public Health, The University of Newcastle</s1>
<s3>AUS</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
</inist:fA14>
</affiliation>
<affiliation><inist:fA14 i1="03"><s1>Department of Respiratory and Sleep Medicine, John Hunter Hospital</s1>
<s2>Newcastle</s2>
<s3>AUS</s3>
<sZ>2 aut.</sZ>
<sZ>4 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
</analytic>
<series><title level="j" type="main">The Journal of infectious diseases</title>
<title level="j" type="abbreviated">J. infect. dis.</title>
<idno type="ISSN">0022-1899</idno>
<imprint><date when="2013">2013</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><title level="j" type="main">The Journal of infectious diseases</title>
<title level="j" type="abbreviated">J. infect. dis.</title>
<idno type="ISSN">0022-1899</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>CD8 T lymphocyte</term>
<term>Dendritic cell</term>
<term>Female</term>
<term>Human</term>
<term>Infection</term>
<term>Influenzavirus A(H1N1)</term>
<term>Phenotype</term>
<term>Pregnancy</term>
<term>Woman</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Homme</term>
<term>Cellule dendritique</term>
<term>Lymphocyte T CD8</term>
<term>Gestation</term>
<term>Femelle</term>
<term>Femme</term>
<term>Phénotype</term>
<term>Infection</term>
<term>Virus grippal A(H1N1)</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Background. Pregnant women are a high-risk group during influenza pandemics. In this study we determined whether plasmacytoid dendritic cells (pDCs) and CD8 T cells from pregnant women display altered activity following in vitro infection with 2009 pandemic swine influenza (H1N1/09). Methods. Peripheral blood mononuclear cells (PBMCs) were isolated from pregnant (n = 26) and nonpregnant (n =<sup> </sup>
28) women. DC subtypes were enumerated from PBMCs. PBMCs were infected with H1N1/09 and CD86, human leukocyte antigen-DR (HLA-DR), and programmed death ligand 1/2 (PDL1/2) measured on pDCs. PD receptor 1 (PD1) was measured on CD8 T cells. Interferon-alpha (IFN-α), interleukin-2 (IL-2), tumor necrosis factor-alpha (TNFα), and IFN-gamma were measured from culture supernatant. Results. pDC (ie, CD303<sup>+</sup>
/CD1c<sup>-</sup>
PBMCs) percentages were lower in pregnant compared with nonpregnant women (P < .05). Following H1N1/09 infection, pDCs from pregnant women showed higher expression of CD86 (P < .01), HLA-DR (P < .001), and PDL1 (P < .001) compared with nonpregnant women. Expression of PD1 on CD8 T cells was higher during pregnancy (P < .05). Following H1N1/09 infection, PBMCs from pregnant women displayed reduced IFN-a (P < .01), IL-2 (P < .01), and IFN-γ (P < .01) compared with nonpregnant women. Blocking PDL1 during H1N1/09 infection increased these cytokines during pregnancy (P < .05). Conclusion. Altered maternal cellular antiviral activity is implicated in the increased morbidity during pregnancy following influenza pandemics.</div>
</front>
</TEI>
<inist><standard h6="B"><pA><fA01 i1="01" i2="1"><s0>0022-1899</s0>
</fA01>
<fA02 i1="01"><s0>JIDIAQ</s0>
</fA02>
<fA03 i2="1"><s0>J. infect. dis.</s0>
</fA03>
<fA05><s2>208</s2>
</fA05>
<fA06><s2>7</s2>
</fA06>
<fA08 i1="01" i2="1" l="ENG"><s1>Plasmacytoid Dendritic Cells and CD8 T Cells From Pregnant Women Show Altered Phenotype and Function Following H1N1/09 Infection</s1>
</fA08>
<fA11 i1="01" i2="1"><s1>VANDERS (Rebecca L.)</s1>
</fA11>
<fA11 i1="02" i2="1"><s1>GIBSON (Peter G.)</s1>
</fA11>
<fA11 i1="03" i2="1"><s1>MURPHY (Vanessa E.)</s1>
</fA11>
<fA11 i1="04" i2="1"><s1>WARK (Peter A. B.)</s1>
</fA11>
<fA14 i1="01"><s1>Centre for Asthma and Respiratory Diseases, School of Medicine and Public Health, The University of Newcastle</s1>
<s3>AUS</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
</fA14>
<fA14 i1="02"><s1>Hunter Medical Research Institute, John Hunter Hospital</s1>
<s2>Newcastle</s2>
<s3>AUS</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
</fA14>
<fA14 i1="03"><s1>Department of Respiratory and Sleep Medicine, John Hunter Hospital</s1>
<s2>Newcastle</s2>
<s3>AUS</s3>
<sZ>2 aut.</sZ>
<sZ>4 aut.</sZ>
</fA14>
<fA20><s1>1062-1070</s1>
</fA20>
<fA21><s1>2013</s1>
</fA21>
<fA23 i1="01"><s0>ENG</s0>
</fA23>
<fA43 i1="01"><s1>INIST</s1>
<s2>2052</s2>
<s5>354000505852320040</s5>
</fA43>
<fA44><s0>0000</s0>
<s1>© 2013 INIST-CNRS. All rights reserved.</s1>
</fA44>
<fA45><s0>42 ref.</s0>
</fA45>
<fA47 i1="01" i2="1"><s0>13-0310021</s0>
</fA47>
<fA60><s1>P</s1>
</fA60>
<fA61><s0>A</s0>
</fA61>
<fA64 i1="01" i2="1"><s0>The Journal of infectious diseases</s0>
</fA64>
<fA66 i1="01"><s0>GBR</s0>
</fA66>
<fC01 i1="01" l="ENG"><s0>Background. Pregnant women are a high-risk group during influenza pandemics. In this study we determined whether plasmacytoid dendritic cells (pDCs) and CD8 T cells from pregnant women display altered activity following in vitro infection with 2009 pandemic swine influenza (H1N1/09). Methods. Peripheral blood mononuclear cells (PBMCs) were isolated from pregnant (n = 26) and nonpregnant (n =<sup> </sup>
28) women. DC subtypes were enumerated from PBMCs. PBMCs were infected with H1N1/09 and CD86, human leukocyte antigen-DR (HLA-DR), and programmed death ligand 1/2 (PDL1/2) measured on pDCs. PD receptor 1 (PD1) was measured on CD8 T cells. Interferon-alpha (IFN-α), interleukin-2 (IL-2), tumor necrosis factor-alpha (TNFα), and IFN-gamma were measured from culture supernatant. Results. pDC (ie, CD303<sup>+</sup>
/CD1c<sup>-</sup>
PBMCs) percentages were lower in pregnant compared with nonpregnant women (P < .05). Following H1N1/09 infection, pDCs from pregnant women showed higher expression of CD86 (P < .01), HLA-DR (P < .001), and PDL1 (P < .001) compared with nonpregnant women. Expression of PD1 on CD8 T cells was higher during pregnancy (P < .05). Following H1N1/09 infection, PBMCs from pregnant women displayed reduced IFN-a (P < .01), IL-2 (P < .01), and IFN-γ (P < .01) compared with nonpregnant women. Blocking PDL1 during H1N1/09 infection increased these cytokines during pregnancy (P < .05). Conclusion. Altered maternal cellular antiviral activity is implicated in the increased morbidity during pregnancy following influenza pandemics.</s0>
</fC01>
<fC02 i1="01" i2="X"><s0>002A05</s0>
</fC02>
<fC02 i1="02" i2="X"><s0>002B05</s0>
</fC02>
<fC03 i1="01" i2="X" l="FRE"><s0>Homme</s0>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="ENG"><s0>Human</s0>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="SPA"><s0>Hombre</s0>
<s5>01</s5>
</fC03>
<fC03 i1="02" i2="X" l="FRE"><s0>Cellule dendritique</s0>
<s5>05</s5>
</fC03>
<fC03 i1="02" i2="X" l="ENG"><s0>Dendritic cell</s0>
<s5>05</s5>
</fC03>
<fC03 i1="02" i2="X" l="SPA"><s0>Célula dendrítica</s0>
<s5>05</s5>
</fC03>
<fC03 i1="03" i2="X" l="FRE"><s0>Lymphocyte T CD8</s0>
<s5>06</s5>
</fC03>
<fC03 i1="03" i2="X" l="ENG"><s0>CD8 T lymphocyte</s0>
<s5>06</s5>
</fC03>
<fC03 i1="03" i2="X" l="SPA"><s0>Linfocito T CD8</s0>
<s5>06</s5>
</fC03>
<fC03 i1="04" i2="X" l="FRE"><s0>Gestation</s0>
<s5>07</s5>
</fC03>
<fC03 i1="04" i2="X" l="ENG"><s0>Pregnancy</s0>
<s5>07</s5>
</fC03>
<fC03 i1="04" i2="X" l="SPA"><s0>Gestación</s0>
<s5>07</s5>
</fC03>
<fC03 i1="05" i2="X" l="FRE"><s0>Femelle</s0>
<s5>08</s5>
</fC03>
<fC03 i1="05" i2="X" l="ENG"><s0>Female</s0>
<s5>08</s5>
</fC03>
<fC03 i1="05" i2="X" l="SPA"><s0>Hembra</s0>
<s5>08</s5>
</fC03>
<fC03 i1="06" i2="X" l="FRE"><s0>Femme</s0>
<s5>09</s5>
</fC03>
<fC03 i1="06" i2="X" l="ENG"><s0>Woman</s0>
<s5>09</s5>
</fC03>
<fC03 i1="06" i2="X" l="SPA"><s0>Mujer</s0>
<s5>09</s5>
</fC03>
<fC03 i1="07" i2="X" l="FRE"><s0>Phénotype</s0>
<s5>10</s5>
</fC03>
<fC03 i1="07" i2="X" l="ENG"><s0>Phenotype</s0>
<s5>10</s5>
</fC03>
<fC03 i1="07" i2="X" l="SPA"><s0>Fenotipo</s0>
<s5>10</s5>
</fC03>
<fC03 i1="08" i2="X" l="FRE"><s0>Infection</s0>
<s5>11</s5>
</fC03>
<fC03 i1="08" i2="X" l="ENG"><s0>Infection</s0>
<s5>11</s5>
</fC03>
<fC03 i1="08" i2="X" l="SPA"><s0>Infección</s0>
<s5>11</s5>
</fC03>
<fC03 i1="09" i2="X" l="FRE"><s0>Virus grippal A(H1N1)</s0>
<s4>CD</s4>
<s5>96</s5>
</fC03>
<fC03 i1="09" i2="X" l="ENG"><s0>Influenzavirus A(H1N1)</s0>
<s4>CD</s4>
<s5>96</s5>
</fC03>
<fN21><s1>294</s1>
</fN21>
<fN44 i1="01"><s1>OTO</s1>
</fN44>
<fN82><s1>OTO</s1>
</fN82>
</pA>
</standard>
<server><NO>PASCAL 13-0310021 INIST</NO>
<ET>Plasmacytoid Dendritic Cells and CD8 T Cells From Pregnant Women Show Altered Phenotype and Function Following H1N1/09 Infection</ET>
<AU>VANDERS (Rebecca L.); GIBSON (Peter G.); MURPHY (Vanessa E.); WARK (Peter A. B.)</AU>
<AF>Centre for Asthma and Respiratory Diseases, School of Medicine and Public Health, The University of Newcastle/Australie (1 aut., 2 aut., 3 aut., 4 aut.); Hunter Medical Research Institute, John Hunter Hospital/Newcastle/Australie (1 aut., 3 aut.); Department of Respiratory and Sleep Medicine, John Hunter Hospital/Newcastle/Australie (2 aut., 4 aut.)</AF>
<DT>Publication en série; Niveau analytique</DT>
<SO>The Journal of infectious diseases; ISSN 0022-1899; Coden JIDIAQ; Royaume-Uni; Da. 2013; Vol. 208; No. 7; Pp. 1062-1070; Bibl. 42 ref.</SO>
<LA>Anglais</LA>
<EA>Background. Pregnant women are a high-risk group during influenza pandemics. In this study we determined whether plasmacytoid dendritic cells (pDCs) and CD8 T cells from pregnant women display altered activity following in vitro infection with 2009 pandemic swine influenza (H1N1/09). Methods. Peripheral blood mononuclear cells (PBMCs) were isolated from pregnant (n = 26) and nonpregnant (n =<sup> </sup>
28) women. DC subtypes were enumerated from PBMCs. PBMCs were infected with H1N1/09 and CD86, human leukocyte antigen-DR (HLA-DR), and programmed death ligand 1/2 (PDL1/2) measured on pDCs. PD receptor 1 (PD1) was measured on CD8 T cells. Interferon-alpha (IFN-α), interleukin-2 (IL-2), tumor necrosis factor-alpha (TNFα), and IFN-gamma were measured from culture supernatant. Results. pDC (ie, CD303<sup>+</sup>
/CD1c<sup>-</sup>
PBMCs) percentages were lower in pregnant compared with nonpregnant women (P < .05). Following H1N1/09 infection, pDCs from pregnant women showed higher expression of CD86 (P < .01), HLA-DR (P < .001), and PDL1 (P < .001) compared with nonpregnant women. Expression of PD1 on CD8 T cells was higher during pregnancy (P < .05). Following H1N1/09 infection, PBMCs from pregnant women displayed reduced IFN-a (P < .01), IL-2 (P < .01), and IFN-γ (P < .01) compared with nonpregnant women. Blocking PDL1 during H1N1/09 infection increased these cytokines during pregnancy (P < .05). Conclusion. Altered maternal cellular antiviral activity is implicated in the increased morbidity during pregnancy following influenza pandemics.</EA>
<CC>002A05; 002B05</CC>
<FD>Homme; Cellule dendritique; Lymphocyte T CD8; Gestation; Femelle; Femme; Phénotype; Infection; Virus grippal A(H1N1)</FD>
<ED>Human; Dendritic cell; CD8 T lymphocyte; Pregnancy; Female; Woman; Phenotype; Infection; Influenzavirus A(H1N1)</ED>
<SD>Hombre; Célula dendrítica; Linfocito T CD8; Gestación; Hembra; Mujer; Fenotipo; Infección</SD>
<LO>INIST-2052.354000505852320040</LO>
<ID>13-0310021</ID>
</server>
</inist>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/PandemieGrippaleV1/Data/PascalFrancis/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000221 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/PascalFrancis/Corpus/biblio.hfd -nk 000221 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= PandemieGrippaleV1 |flux= PascalFrancis |étape= Corpus |type= RBID |clé= Pascal:13-0310021 |texte= Plasmacytoid Dendritic Cells and CD8 T Cells From Pregnant Women Show Altered Phenotype and Function Following H1N1/09 Infection }}
This area was generated with Dilib version V0.6.34. |