PandemieGrippaleV1, PascalFrancis, Corpus, bibRecord, 000221

Plasmacytoid Dendritic Cells and CD8 T Cells From Pregnant Women Show Altered Phenotype and Function Following H1N1/09 Infection

Identifieur interne : 000221 ( PascalFrancis/Corpus ); précédent : 000220; suivant : 000222

Plasmacytoid Dendritic Cells and CD8 T Cells From Pregnant Women Show Altered Phenotype and Function Following H1N1/09 Infection

Auteurs : Rebecca L. Vanders ; Peter G. Gibson ; Vanessa E. Murphy ; Peter A. B. Wark

Source :

The Journal of infectious diseases [ 0022-1899 ] ; 2013.

RBID : Pascal:13-0310021

Descripteurs français

Pascal (Inist)
- Homme, Cellule dendritique, Lymphocyte T CD8, Gestation, Femelle, Femme, Phénotype, Infection, Virus grippal A(H1N1).

English descriptors

KwdEn :
- CD8 T lymphocyte, Dendritic cell, Female, Human, Infection, Influenzavirus A(H1N1), Phenotype, Pregnancy, Woman.

Abstract

Background. Pregnant women are a high-risk group during influenza pandemics. In this study we determined whether plasmacytoid dendritic cells (pDCs) and CD8 T cells from pregnant women display altered activity following in vitro infection with 2009 pandemic swine influenza (H1N1/09). Methods. Peripheral blood mononuclear cells (PBMCs) were isolated from pregnant (n = 26) and nonpregnant (n =28) women. DC subtypes were enumerated from PBMCs. PBMCs were infected with H1N1/09 and CD86, human leukocyte antigen-DR (HLA-DR), and programmed death ligand 1/2 (PDL1/2) measured on pDCs. PD receptor 1 (PD1) was measured on CD8 T cells. Interferon-alpha (IFN-α), interleukin-2 (IL-2), tumor necrosis factor-alpha (TNFα), and IFN-gamma were measured from culture supernatant. Results. pDC (ie, CD303⁺/CD1c^- PBMCs) percentages were lower in pregnant compared with nonpregnant women (P < .05). Following H1N1/09 infection, pDCs from pregnant women showed higher expression of CD86 (P < .01), HLA-DR (P < .001), and PDL1 (P < .001) compared with nonpregnant women. Expression of PD1 on CD8 T cells was higher during pregnancy (P < .05). Following H1N1/09 infection, PBMCs from pregnant women displayed reduced IFN-a (P < .01), IL-2 (P < .01), and IFN-γ (P < .01) compared with nonpregnant women. Blocking PDL1 during H1N1/09 infection increased these cytokines during pregnancy (P < .05). Conclusion. Altered maternal cellular antiviral activity is implicated in the increased morbidity during pregnancy following influenza pandemics.

Notice en format standard (ISO 2709)

Pour connaître la documentation sur le format Inist Standard.

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A02	`01`			`@0 JIDIAQ`
A03		`1`		`@0 J. infect. dis.`
A05				`@2 208`
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A08	`01`	`1`	`ENG`	`@1 Plasmacytoid Dendritic Cells and CD8 T Cells From Pregnant Women Show Altered Phenotype and Function Following H1N1/09 Infection`
A11	`01`	`1`		`@1 VANDERS (Rebecca L.)`
A11	`02`	`1`		`@1 GIBSON (Peter G.)`
A11	`03`	`1`		`@1 MURPHY (Vanessa E.)`
A11	`04`	`1`		`@1 WARK (Peter A. B.)`
A14	`01`			`@1 Centre for Asthma and Respiratory Diseases, School of Medicine and Public Health, The University of Newcastle @3 AUS @Z 1 aut. @Z 2 aut. @Z 3 aut. @Z 4 aut.`
A14	`02`			`@1 Hunter Medical Research Institute, John Hunter Hospital @2 Newcastle @3 AUS @Z 1 aut. @Z 3 aut.`
A14	`03`			`@1 Department of Respiratory and Sleep Medicine, John Hunter Hospital @2 Newcastle @3 AUS @Z 2 aut. @Z 4 aut.`
A20				`@1 1062-1070`
A21				`@1 2013`
A23	`01`			`@0 ENG`
A43	`01`			`@1 INIST @2 2052 @5 354000505852320040`
A44				`@0 0000 @1 © 2013 INIST-CNRS. All rights reserved.`
A45				`@0 42 ref.`
A47	`01`	`1`		`@0 13-0310021`
A60				`@1 P`
A61				`@0 A`
A64	`01`	`1`		`@0 The Journal of infectious diseases`
A66	`01`			`@0 GBR`
C01	`01`		`ENG`	@0 Background. Pregnant women are a high-risk group during influenza pandemics. In this study we determined whether plasmacytoid dendritic cells (pDCs) and CD8 T cells from pregnant women display altered activity following in vitro infection with 2009 pandemic swine influenza (H1N1/09). Methods. Peripheral blood mononuclear cells (PBMCs) were isolated from pregnant (n = 26) and nonpregnant (n =28) women. DC subtypes were enumerated from PBMCs. PBMCs were infected with H1N1/09 and CD86, human leukocyte antigen-DR (HLA-DR), and programmed death ligand 1/2 (PDL1/2) measured on pDCs. PD receptor 1 (PD1) was measured on CD8 T cells. Interferon-alpha (IFN-α), interleukin-2 (IL-2), tumor necrosis factor-alpha (TNFα), and IFN-gamma were measured from culture supernatant. Results. pDC (ie, CD303⁺/CD1c^- PBMCs) percentages were lower in pregnant compared with nonpregnant women (P < .05). Following H1N1/09 infection, pDCs from pregnant women showed higher expression of CD86 (P < .01), HLA-DR (P < .001), and PDL1 (P < .001) compared with nonpregnant women. Expression of PD1 on CD8 T cells was higher during pregnancy (P < .05). Following H1N1/09 infection, PBMCs from pregnant women displayed reduced IFN-a (P < .01), IL-2 (P < .01), and IFN-γ (P < .01) compared with nonpregnant women. Blocking PDL1 during H1N1/09 infection increased these cytokines during pregnancy (P < .05). Conclusion. Altered maternal cellular antiviral activity is implicated in the increased morbidity during pregnancy following influenza pandemics.
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C03	`01`	`X`	`ENG`	`@0 Human @5 01`
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C03	`02`	`X`	`ENG`	`@0 Dendritic cell @5 05`
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C03	`03`	`X`	`ENG`	`@0 CD8 T lymphocyte @5 06`
C03	`03`	`X`	`SPA`	`@0 Linfocito T CD8 @5 06`
C03	`04`	`X`	`FRE`	`@0 Gestation @5 07`
C03	`04`	`X`	`ENG`	`@0 Pregnancy @5 07`
C03	`04`	`X`	`SPA`	`@0 Gestación @5 07`
C03	`05`	`X`	`FRE`	`@0 Femelle @5 08`
C03	`05`	`X`	`ENG`	`@0 Female @5 08`
C03	`05`	`X`	`SPA`	`@0 Hembra @5 08`
C03	`06`	`X`	`FRE`	`@0 Femme @5 09`
C03	`06`	`X`	`ENG`	`@0 Woman @5 09`
C03	`06`	`X`	`SPA`	`@0 Mujer @5 09`
C03	`07`	`X`	`FRE`	`@0 Phénotype @5 10`
C03	`07`	`X`	`ENG`	`@0 Phenotype @5 10`
C03	`07`	`X`	`SPA`	`@0 Fenotipo @5 10`
C03	`08`	`X`	`FRE`	`@0 Infection @5 11`
C03	`08`	`X`	`ENG`	`@0 Infection @5 11`
C03	`08`	`X`	`SPA`	`@0 Infección @5 11`
C03	`09`	`X`	`FRE`	`@0 Virus grippal A(H1N1) @4 CD @5 96`
C03	`09`	`X`	`ENG`	`@0 Influenzavirus A(H1N1) @4 CD @5 96`
N21				`@1 294`
N44	`01`			`@1 OTO`
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Format Inist (serveur)

NO :	PASCAL 13-0310021 INIST
ET :	Plasmacytoid Dendritic Cells and CD8 T Cells From Pregnant Women Show Altered Phenotype and Function Following H1N1/09 Infection
AU :	VANDERS (Rebecca L.); GIBSON (Peter G.); MURPHY (Vanessa E.); WARK (Peter A. B.)
AF :	Centre for Asthma and Respiratory Diseases, School of Medicine and Public Health, The University of Newcastle/Australie (1 aut., 2 aut., 3 aut., 4 aut.); Hunter Medical Research Institute, John Hunter Hospital/Newcastle/Australie (1 aut., 3 aut.); Department of Respiratory and Sleep Medicine, John Hunter Hospital/Newcastle/Australie (2 aut., 4 aut.)
DT :	Publication en série; Niveau analytique
SO :	The Journal of infectious diseases; ISSN 0022-1899; Coden JIDIAQ; Royaume-Uni; Da. 2013; Vol. 208; No. 7; Pp. 1062-1070; Bibl. 42 ref.
LA :	Anglais
EA :	Background. Pregnant women are a high-risk group during influenza pandemics. In this study we determined whether plasmacytoid dendritic cells (pDCs) and CD8 T cells from pregnant women display altered activity following in vitro infection with 2009 pandemic swine influenza (H1N1/09). Methods. Peripheral blood mononuclear cells (PBMCs) were isolated from pregnant (n = 26) and nonpregnant (n =28) women. DC subtypes were enumerated from PBMCs. PBMCs were infected with H1N1/09 and CD86, human leukocyte antigen-DR (HLA-DR), and programmed death ligand 1/2 (PDL1/2) measured on pDCs. PD receptor 1 (PD1) was measured on CD8 T cells. Interferon-alpha (IFN-α), interleukin-2 (IL-2), tumor necrosis factor-alpha (TNFα), and IFN-gamma were measured from culture supernatant. Results. pDC (ie, CD303⁺/CD1c^- PBMCs) percentages were lower in pregnant compared with nonpregnant women (P < .05). Following H1N1/09 infection, pDCs from pregnant women showed higher expression of CD86 (P < .01), HLA-DR (P < .001), and PDL1 (P < .001) compared with nonpregnant women. Expression of PD1 on CD8 T cells was higher during pregnancy (P < .05). Following H1N1/09 infection, PBMCs from pregnant women displayed reduced IFN-a (P < .01), IL-2 (P < .01), and IFN-γ (P < .01) compared with nonpregnant women. Blocking PDL1 during H1N1/09 infection increased these cytokines during pregnancy (P < .05). Conclusion. Altered maternal cellular antiviral activity is implicated in the increased morbidity during pregnancy following influenza pandemics.
CC :	002A05; 002B05
FD :	Homme; Cellule dendritique; Lymphocyte T CD8; Gestation; Femelle; Femme; Phénotype; Infection; Virus grippal A(H1N1)
ED :	Human; Dendritic cell; CD8 T lymphocyte; Pregnancy; Female; Woman; Phenotype; Infection; Influenzavirus A(H1N1)
SD :	Hombre; Célula dendrítica; Linfocito T CD8; Gestación; Hembra; Mujer; Fenotipo; Infección
LO :	INIST-2052.354000505852320040
ID :	13-0310021

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Pascal:13-0310021

Le document en format XML

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<author><name sortKey="Gibson, Peter G" sort="Gibson, Peter G" uniqKey="Gibson P" first="Peter G." last="Gibson">Peter G. Gibson</name>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>CD8 T lymphocyte</term>
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<term>Female</term>
<term>Human</term>
<term>Infection</term>
<term>Influenzavirus A(H1N1)</term>
<term>Phenotype</term>
<term>Pregnancy</term>
<term>Woman</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Homme</term>
<term>Cellule dendritique</term>
<term>Lymphocyte T CD8</term>
<term>Gestation</term>
<term>Femelle</term>
<term>Femme</term>
<term>Phénotype</term>
<term>Infection</term>
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<front><div type="abstract" xml:lang="en">Background. Pregnant women are a high-risk group during influenza pandemics. In this study we determined whether plasmacytoid dendritic cells (pDCs) and CD8 T cells from pregnant women display altered activity following in vitro infection with 2009 pandemic swine influenza (H1N1/09). Methods. Peripheral blood mononuclear cells (PBMCs) were isolated from pregnant (n = 26) and nonpregnant (n =<sup> </sup>
28) women. DC subtypes were enumerated from PBMCs. PBMCs were infected with H1N1/09 and CD86, human leukocyte antigen-DR (HLA-DR), and programmed death ligand 1/2 (PDL1/2) measured on pDCs. PD receptor 1 (PD1) was measured on CD8 T cells. Interferon-alpha (IFN-α), interleukin-2 (IL-2), tumor necrosis factor-alpha (TNFα), and IFN-gamma were measured from culture supernatant. Results. pDC (ie, CD303<sup>+</sup>
/CD1c<sup>-</sup>
 PBMCs) percentages were lower in pregnant compared with nonpregnant women (P < .05). Following H1N1/09 infection, pDCs from pregnant women showed higher expression of CD86 (P < .01), HLA-DR (P < .001), and PDL1 (P < .001) compared with nonpregnant women. Expression of PD1 on CD8 T cells was higher during pregnancy (P < .05). Following H1N1/09 infection, PBMCs from pregnant women displayed reduced IFN-a (P < .01), IL-2 (P < .01), and IFN-γ (P < .01) compared with nonpregnant women. Blocking PDL1 during H1N1/09 infection increased these cytokines during pregnancy (P < .05). Conclusion. Altered maternal cellular antiviral activity is implicated in the increased morbidity during pregnancy following influenza pandemics.</div>
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<fA11 i1="01" i2="1"><s1>VANDERS (Rebecca L.)</s1>
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<fA11 i1="02" i2="1"><s1>GIBSON (Peter G.)</s1>
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<fA11 i1="03" i2="1"><s1>MURPHY (Vanessa E.)</s1>
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<fA11 i1="04" i2="1"><s1>WARK (Peter A. B.)</s1>
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<fA14 i1="01"><s1>Centre for Asthma and Respiratory Diseases, School of Medicine and Public Health, The University of Newcastle</s1>
<s3>AUS</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
</fA14>
<fA14 i1="02"><s1>Hunter Medical Research Institute, John Hunter Hospital</s1>
<s2>Newcastle</s2>
<s3>AUS</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
</fA14>
<fA14 i1="03"><s1>Department of Respiratory and Sleep Medicine, John Hunter Hospital</s1>
<s2>Newcastle</s2>
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<sZ>4 aut.</sZ>
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<fC01 i1="01" l="ENG"><s0>Background. Pregnant women are a high-risk group during influenza pandemics. In this study we determined whether plasmacytoid dendritic cells (pDCs) and CD8 T cells from pregnant women display altered activity following in vitro infection with 2009 pandemic swine influenza (H1N1/09). Methods. Peripheral blood mononuclear cells (PBMCs) were isolated from pregnant (n = 26) and nonpregnant (n =<sup> </sup>
28) women. DC subtypes were enumerated from PBMCs. PBMCs were infected with H1N1/09 and CD86, human leukocyte antigen-DR (HLA-DR), and programmed death ligand 1/2 (PDL1/2) measured on pDCs. PD receptor 1 (PD1) was measured on CD8 T cells. Interferon-alpha (IFN-α), interleukin-2 (IL-2), tumor necrosis factor-alpha (TNFα), and IFN-gamma were measured from culture supernatant. Results. pDC (ie, CD303<sup>+</sup>
/CD1c<sup>-</sup>
 PBMCs) percentages were lower in pregnant compared with nonpregnant women (P < .05). Following H1N1/09 infection, pDCs from pregnant women showed higher expression of CD86 (P < .01), HLA-DR (P < .001), and PDL1 (P < .001) compared with nonpregnant women. Expression of PD1 on CD8 T cells was higher during pregnancy (P < .05). Following H1N1/09 infection, PBMCs from pregnant women displayed reduced IFN-a (P < .01), IL-2 (P < .01), and IFN-γ (P < .01) compared with nonpregnant women. Blocking PDL1 during H1N1/09 infection increased these cytokines during pregnancy (P < .05). Conclusion. Altered maternal cellular antiviral activity is implicated in the increased morbidity during pregnancy following influenza pandemics.</s0>
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<server><NO>PASCAL 13-0310021 INIST</NO>
<ET>Plasmacytoid Dendritic Cells and CD8 T Cells From Pregnant Women Show Altered Phenotype and Function Following H1N1/09 Infection</ET>
<AU>VANDERS (Rebecca L.); GIBSON (Peter G.); MURPHY (Vanessa E.); WARK (Peter A. B.)</AU>
<AF>Centre for Asthma and Respiratory Diseases, School of Medicine and Public Health, The University of Newcastle/Australie (1 aut., 2 aut., 3 aut., 4 aut.); Hunter Medical Research Institute, John Hunter Hospital/Newcastle/Australie (1 aut., 3 aut.); Department of Respiratory and Sleep Medicine, John Hunter Hospital/Newcastle/Australie (2 aut., 4 aut.)</AF>
<DT>Publication en série; Niveau analytique</DT>
<SO>The Journal of infectious diseases; ISSN 0022-1899; Coden JIDIAQ; Royaume-Uni; Da. 2013; Vol. 208; No. 7; Pp. 1062-1070; Bibl. 42 ref.</SO>
<LA>Anglais</LA>
<EA>Background. Pregnant women are a high-risk group during influenza pandemics. In this study we determined whether plasmacytoid dendritic cells (pDCs) and CD8 T cells from pregnant women display altered activity following in vitro infection with 2009 pandemic swine influenza (H1N1/09). Methods. Peripheral blood mononuclear cells (PBMCs) were isolated from pregnant (n = 26) and nonpregnant (n =<sup> </sup>
28) women. DC subtypes were enumerated from PBMCs. PBMCs were infected with H1N1/09 and CD86, human leukocyte antigen-DR (HLA-DR), and programmed death ligand 1/2 (PDL1/2) measured on pDCs. PD receptor 1 (PD1) was measured on CD8 T cells. Interferon-alpha (IFN-α), interleukin-2 (IL-2), tumor necrosis factor-alpha (TNFα), and IFN-gamma were measured from culture supernatant. Results. pDC (ie, CD303<sup>+</sup>
/CD1c<sup>-</sup>
 PBMCs) percentages were lower in pregnant compared with nonpregnant women (P < .05). Following H1N1/09 infection, pDCs from pregnant women showed higher expression of CD86 (P < .01), HLA-DR (P < .001), and PDL1 (P < .001) compared with nonpregnant women. Expression of PD1 on CD8 T cells was higher during pregnancy (P < .05). Following H1N1/09 infection, PBMCs from pregnant women displayed reduced IFN-a (P < .01), IL-2 (P < .01), and IFN-γ (P < .01) compared with nonpregnant women. Blocking PDL1 during H1N1/09 infection increased these cytokines during pregnancy (P < .05). Conclusion. Altered maternal cellular antiviral activity is implicated in the increased morbidity during pregnancy following influenza pandemics.</EA>
<CC>002A05; 002B05</CC>
<FD>Homme; Cellule dendritique; Lymphocyte T CD8; Gestation; Femelle; Femme; Phénotype; Infection; Virus grippal A(H1N1)</FD>
<ED>Human; Dendritic cell; CD8 T lymphocyte; Pregnancy; Female; Woman; Phenotype; Infection; Influenzavirus A(H1N1)</ED>
<SD>Hombre; Célula dendrítica; Linfocito T CD8; Gestación; Hembra; Mujer; Fenotipo; Infección</SD>
<LO>INIST-2052.354000505852320040</LO>
<ID>13-0310021</ID>
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Plasmacytoid Dendritic Cells and CD8 T Cells From Pregnant Women Show Altered Phenotype and Function Following H1N1/09 Infection

Plasmacytoid Dendritic Cells and CD8 T Cells From Pregnant Women Show Altered Phenotype and Function Following H1N1/09 Infection

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