Passive immunization with a recombinant adenovirus expressing an HA (H5)-specific single-domain antibody protects mice from lethal influenza infection
Identifieur interne : 000216 ( PascalFrancis/Checkpoint ); précédent : 000215; suivant : 000217Passive immunization with a recombinant adenovirus expressing an HA (H5)-specific single-domain antibody protects mice from lethal influenza infection
Auteurs : Irina L. Tutykhina [Russie] ; Elena S. Sedova [Russie] ; Irina Y. Gribova [Russie] ; Tatiana I. Ivanova [Russie] ; Lev A. Vasilev [Russie] ; Marina V. Rutovskaya [Russie] ; Andrei A. Lysenko [Russie] ; Maxim M. Shmarov [Russie] ; Denis Y. Logunov [Russie] ; Boris S. Naroditsky [Russie] ; Sergei V. Tillib [Russie] ; Alexander L. Gintsburg [Russie]Source :
- Antiviral research [ 0166-3542 ] ; 2013.
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Abstract
One effective method for the prevention and treatment of influenza infection is passive immunization. In our study, we examined the feasibility of creating an antibody-based preparation with a prolonged protective effect against influenza virus. Single-domain antibodies (sdAbs) specific for influenza virus hem-agglutinin were generated. Experiments in mouse models showed 100% survivability for both intranasal sdAbs administration 24 h prior to influenza challenge and 24 h after infection. sdAb-gene delivery by an adenoviral vector led to gene expression for up to 14 days. Protection by a recombinant adenovirus containing the sdAb gene was observed in cases of administration prior to influenza infection (14 d-24 h). We also demonstrated that the single administration of a combined preparation containing sdAb DNA and protein expanded the protection time window from 14 d prior to 48 h after influenza infection. This approach and the application of a broad-spectrum sdAbs will allow the development of efficient drugs for the prevention and treatment of viral infections produced by pandemic virus variants and other infections.
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">Passive immunization with a recombinant adenovirus expressing an HA (H5)-specific single-domain antibody protects mice from lethal influenza infection</title><author><name sortKey="Tutykhina, Irina L" sort="Tutykhina, Irina L" uniqKey="Tutykhina I" first="Irina L." last="Tutykhina">Irina L. Tutykhina</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Gamaleya Research Institute for Epidemiology and Microbiology, 18, Gamaleya Street</s1><s2>Moscow 123098</s2><s3>RUS</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>Russie</country><placeName><settlement type="city">Moscou</settlement><region>District fédéral central</region></placeName></affiliation></author><author><name sortKey="Sedova, Elena S" sort="Sedova, Elena S" uniqKey="Sedova E" first="Elena S." last="Sedova">Elena S. Sedova</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Gamaleya Research Institute for Epidemiology and Microbiology, 18, Gamaleya Street</s1><s2>Moscow 123098</s2><s3>RUS</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>Russie</country><placeName><settlement type="city">Moscou</settlement><region>District fédéral central</region></placeName></affiliation></author><author><name sortKey="Gribova, Irina Y" sort="Gribova, Irina Y" uniqKey="Gribova I" first="Irina Y." last="Gribova">Irina Y. Gribova</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Gamaleya Research Institute for Epidemiology and Microbiology, 18, Gamaleya Street</s1><s2>Moscow 123098</s2><s3>RUS</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>Russie</country><placeName><settlement type="city">Moscou</settlement><region>District fédéral central</region></placeName></affiliation></author><author><name sortKey="Ivanova, Tatiana I" sort="Ivanova, Tatiana I" uniqKey="Ivanova T" first="Tatiana I." last="Ivanova">Tatiana I. Ivanova</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Institute of Gene Biology, Russian Academy of Sciences, 34/5, Vavilova Street</s1><s2>Moscow 119334</s2><s3>RUS</s3><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>Russie</country><placeName><settlement type="city">Moscou</settlement><region>District fédéral central</region></placeName></affiliation></author><author><name sortKey="Vasilev, Lev A" sort="Vasilev, Lev A" uniqKey="Vasilev L" first="Lev A." last="Vasilev">Lev A. Vasilev</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Institute of Gene Biology, Russian Academy of Sciences, 34/5, Vavilova Street</s1><s2>Moscow 119334</s2><s3>RUS</s3><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>Russie</country><placeName><settlement type="city">Moscou</settlement><region>District fédéral central</region></placeName></affiliation></author><author><name sortKey="Rutovskaya, Marina V" sort="Rutovskaya, Marina V" uniqKey="Rutovskaya M" first="Marina V." last="Rutovskaya">Marina V. Rutovskaya</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Institute of Gene Biology, Russian Academy of Sciences, 34/5, Vavilova Street</s1><s2>Moscow 119334</s2><s3>RUS</s3><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>Russie</country><placeName><settlement type="city">Moscou</settlement><region>District fédéral central</region></placeName></affiliation></author><author><name sortKey="Lysenko, Andrei A" sort="Lysenko, Andrei A" uniqKey="Lysenko A" first="Andrei A." last="Lysenko">Andrei A. Lysenko</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Gamaleya Research Institute for Epidemiology and Microbiology, 18, Gamaleya Street</s1><s2>Moscow 123098</s2><s3>RUS</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>Russie</country><placeName><settlement type="city">Moscou</settlement><region>District fédéral central</region></placeName></affiliation></author><author><name sortKey="Shmarov, Maxim M" sort="Shmarov, Maxim M" uniqKey="Shmarov M" first="Maxim M." last="Shmarov">Maxim M. Shmarov</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Gamaleya Research Institute for Epidemiology and Microbiology, 18, Gamaleya Street</s1><s2>Moscow 123098</s2><s3>RUS</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>Russie</country><placeName><settlement type="city">Moscou</settlement><region>District fédéral central</region></placeName></affiliation></author><author><name sortKey="Logunov, Denis Y" sort="Logunov, Denis Y" uniqKey="Logunov D" first="Denis Y." last="Logunov">Denis Y. Logunov</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Gamaleya Research Institute for Epidemiology and Microbiology, 18, Gamaleya Street</s1><s2>Moscow 123098</s2><s3>RUS</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>Russie</country><placeName><settlement type="city">Moscou</settlement><region>District fédéral central</region></placeName></affiliation></author><author><name sortKey="Naroditsky, Boris S" sort="Naroditsky, Boris S" uniqKey="Naroditsky B" first="Boris S." last="Naroditsky">Boris S. Naroditsky</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Gamaleya Research Institute for Epidemiology and Microbiology, 18, Gamaleya Street</s1><s2>Moscow 123098</s2><s3>RUS</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>Russie</country><placeName><settlement type="city">Moscou</settlement><region>District fédéral central</region></placeName></affiliation></author><author><name sortKey="Tillib, Sergei V" sort="Tillib, Sergei V" uniqKey="Tillib S" first="Sergei V." last="Tillib">Sergei V. Tillib</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Institute of Gene Biology, Russian Academy of Sciences, 34/5, Vavilova Street</s1><s2>Moscow 119334</s2><s3>RUS</s3><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>Russie</country><placeName><settlement type="city">Moscou</settlement><region>District fédéral central</region></placeName></affiliation></author><author><name sortKey="Gintsburg, Alexander L" sort="Gintsburg, Alexander L" uniqKey="Gintsburg A" first="Alexander L." last="Gintsburg">Alexander L. Gintsburg</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Gamaleya Research Institute for Epidemiology and Microbiology, 18, Gamaleya Street</s1><s2>Moscow 123098</s2><s3>RUS</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>Russie</country><placeName><settlement type="city">Moscou</settlement><region>District fédéral central</region></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">INIST</idno><idno type="inist">13-0152042</idno><date when="2013">2013</date><idno type="stanalyst">PASCAL 13-0152042 INIST</idno><idno type="RBID">Pascal:13-0152042</idno><idno type="wicri:Area/PascalFrancis/Corpus">000312</idno><idno type="wicri:Area/PascalFrancis/Curation">001B14</idno><idno type="wicri:Area/PascalFrancis/Checkpoint">000216</idno><idno type="wicri:explorRef" wicri:stream="PascalFrancis" wicri:step="Checkpoint">000216</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Passive immunization with a recombinant adenovirus expressing an HA (H5)-specific single-domain antibody protects mice from lethal influenza infection</title><author><name sortKey="Tutykhina, Irina L" sort="Tutykhina, Irina L" uniqKey="Tutykhina I" first="Irina L." last="Tutykhina">Irina L. Tutykhina</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Gamaleya Research Institute for Epidemiology and Microbiology, 18, Gamaleya Street</s1><s2>Moscow 123098</s2><s3>RUS</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>Russie</country><placeName><settlement type="city">Moscou</settlement><region>District fédéral central</region></placeName></affiliation></author><author><name sortKey="Sedova, Elena S" sort="Sedova, Elena S" uniqKey="Sedova E" first="Elena S." last="Sedova">Elena S. Sedova</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Gamaleya Research Institute for Epidemiology and Microbiology, 18, Gamaleya Street</s1><s2>Moscow 123098</s2><s3>RUS</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>Russie</country><placeName><settlement type="city">Moscou</settlement><region>District fédéral central</region></placeName></affiliation></author><author><name sortKey="Gribova, Irina Y" sort="Gribova, Irina Y" uniqKey="Gribova I" first="Irina Y." last="Gribova">Irina Y. Gribova</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Gamaleya Research Institute for Epidemiology and Microbiology, 18, Gamaleya Street</s1><s2>Moscow 123098</s2><s3>RUS</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>Russie</country><placeName><settlement type="city">Moscou</settlement><region>District fédéral central</region></placeName></affiliation></author><author><name sortKey="Ivanova, Tatiana I" sort="Ivanova, Tatiana I" uniqKey="Ivanova T" first="Tatiana I." last="Ivanova">Tatiana I. Ivanova</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Institute of Gene Biology, Russian Academy of Sciences, 34/5, Vavilova Street</s1><s2>Moscow 119334</s2><s3>RUS</s3><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>Russie</country><placeName><settlement type="city">Moscou</settlement><region>District fédéral central</region></placeName></affiliation></author><author><name sortKey="Vasilev, Lev A" sort="Vasilev, Lev A" uniqKey="Vasilev L" first="Lev A." last="Vasilev">Lev A. Vasilev</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Institute of Gene Biology, Russian Academy of Sciences, 34/5, Vavilova Street</s1><s2>Moscow 119334</s2><s3>RUS</s3><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>Russie</country><placeName><settlement type="city">Moscou</settlement><region>District fédéral central</region></placeName></affiliation></author><author><name sortKey="Rutovskaya, Marina V" sort="Rutovskaya, Marina V" uniqKey="Rutovskaya M" first="Marina V." last="Rutovskaya">Marina V. Rutovskaya</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Institute of Gene Biology, Russian Academy of Sciences, 34/5, Vavilova Street</s1><s2>Moscow 119334</s2><s3>RUS</s3><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>Russie</country><placeName><settlement type="city">Moscou</settlement><region>District fédéral central</region></placeName></affiliation></author><author><name sortKey="Lysenko, Andrei A" sort="Lysenko, Andrei A" uniqKey="Lysenko A" first="Andrei A." last="Lysenko">Andrei A. Lysenko</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Gamaleya Research Institute for Epidemiology and Microbiology, 18, Gamaleya Street</s1><s2>Moscow 123098</s2><s3>RUS</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>Russie</country><placeName><settlement type="city">Moscou</settlement><region>District fédéral central</region></placeName></affiliation></author><author><name sortKey="Shmarov, Maxim M" sort="Shmarov, Maxim M" uniqKey="Shmarov M" first="Maxim M." last="Shmarov">Maxim M. Shmarov</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Gamaleya Research Institute for Epidemiology and Microbiology, 18, Gamaleya Street</s1><s2>Moscow 123098</s2><s3>RUS</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>Russie</country><placeName><settlement type="city">Moscou</settlement><region>District fédéral central</region></placeName></affiliation></author><author><name sortKey="Logunov, Denis Y" sort="Logunov, Denis Y" uniqKey="Logunov D" first="Denis Y." last="Logunov">Denis Y. Logunov</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Gamaleya Research Institute for Epidemiology and Microbiology, 18, Gamaleya Street</s1><s2>Moscow 123098</s2><s3>RUS</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>Russie</country><placeName><settlement type="city">Moscou</settlement><region>District fédéral central</region></placeName></affiliation></author><author><name sortKey="Naroditsky, Boris S" sort="Naroditsky, Boris S" uniqKey="Naroditsky B" first="Boris S." last="Naroditsky">Boris S. Naroditsky</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Gamaleya Research Institute for Epidemiology and Microbiology, 18, Gamaleya Street</s1><s2>Moscow 123098</s2><s3>RUS</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>Russie</country><placeName><settlement type="city">Moscou</settlement><region>District fédéral central</region></placeName></affiliation></author><author><name sortKey="Tillib, Sergei V" sort="Tillib, Sergei V" uniqKey="Tillib S" first="Sergei V." last="Tillib">Sergei V. Tillib</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Institute of Gene Biology, Russian Academy of Sciences, 34/5, Vavilova Street</s1><s2>Moscow 119334</s2><s3>RUS</s3><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>Russie</country><placeName><settlement type="city">Moscou</settlement><region>District fédéral central</region></placeName></affiliation></author><author><name sortKey="Gintsburg, Alexander L" sort="Gintsburg, Alexander L" uniqKey="Gintsburg A" first="Alexander L." last="Gintsburg">Alexander L. Gintsburg</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Gamaleya Research Institute for Epidemiology and Microbiology, 18, Gamaleya Street</s1><s2>Moscow 123098</s2><s3>RUS</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>Russie</country><placeName><settlement type="city">Moscou</settlement><region>District fédéral central</region></placeName></affiliation></author></analytic><series><title level="j" type="main">Antiviral research</title><title level="j" type="abbreviated">Antivir. res.</title><idno type="ISSN">0166-3542</idno><imprint><date when="2013">2013</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Antiviral research</title><title level="j" type="abbreviated">Antivir. res.</title><idno type="ISSN">0166-3542</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adenoviridae</term><term>Animal</term><term>Antibody</term><term>Human adenovirus</term><term>Influenza</term><term>Influenzavirus</term><term>Mouse</term><term>Passive immunization</term><term>Prevention</term><term>Vector</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Immunisation passive</term><term>Adenoviridae</term><term>Adénovirus humain</term><term>Anticorps</term><term>Prévention</term><term>Animal</term><term>Souris</term><term>Grippe</term><term>Influenzavirus</term><term>Vecteur</term><term>Forme fatale</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">One effective method for the prevention and treatment of influenza infection is passive immunization. In our study, we examined the feasibility of creating an antibody-based preparation with a prolonged protective effect against influenza virus. Single-domain antibodies (sdAbs) specific for influenza virus hem-agglutinin were generated. Experiments in mouse models showed 100% survivability for both intranasal sdAbs administration 24 h prior to influenza challenge and 24 h after infection. sdAb-gene delivery by an adenoviral vector led to gene expression for up to 14 days. Protection by a recombinant adenovirus containing the sdAb gene was observed in cases of administration prior to influenza infection (14 d-24 h). We also demonstrated that the single administration of a combined preparation containing sdAb DNA and protein expanded the protection time window from 14 d prior to 48 h after influenza infection. This approach and the application of a broad-spectrum sdAbs will allow the development of efficient drugs for the prevention and treatment of viral infections produced by pandemic virus variants and other infections.</div></front></TEI><inist><standard h6="B"><pA><fA01 i1="01" i2="1"><s0>0166-3542</s0></fA01><fA02 i1="01"><s0>ARSRDR</s0></fA02><fA03 i2="1"><s0>Antivir. res.</s0></fA03><fA05><s2>97</s2></fA05><fA06><s2>3</s2></fA06><fA08 i1="01" i2="1" l="ENG"><s1>Passive immunization with a recombinant adenovirus expressing an HA (H5)-specific single-domain antibody protects mice from lethal influenza infection</s1></fA08><fA11 i1="01" i2="1"><s1>TUTYKHINA (Irina L.)</s1></fA11><fA11 i1="02" i2="1"><s1>SEDOVA (Elena S.)</s1></fA11><fA11 i1="03" i2="1"><s1>GRIBOVA (Irina Y.)</s1></fA11><fA11 i1="04" i2="1"><s1>IVANOVA (Tatiana I.)</s1></fA11><fA11 i1="05" i2="1"><s1>VASILEV (Lev A.)</s1></fA11><fA11 i1="06" i2="1"><s1>RUTOVSKAYA (Marina V.)</s1></fA11><fA11 i1="07" i2="1"><s1>LYSENKO (Andrei A.)</s1></fA11><fA11 i1="08" i2="1"><s1>SHMAROV (Maxim M.)</s1></fA11><fA11 i1="09" i2="1"><s1>LOGUNOV (Denis Y.)</s1></fA11><fA11 i1="10" i2="1"><s1>NARODITSKY (Boris S.)</s1></fA11><fA11 i1="11" i2="1"><s1>TILLIB (Sergei V.)</s1></fA11><fA11 i1="12" i2="1"><s1>GINTSBURG (Alexander L.)</s1></fA11><fA14 i1="01"><s1>Gamaleya Research Institute for Epidemiology and Microbiology, 18, Gamaleya Street</s1><s2>Moscow 123098</s2><s3>RUS</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>12 aut.</sZ></fA14><fA14 i1="02"><s1>Institute of Gene Biology, Russian Academy of Sciences, 34/5, Vavilova Street</s1><s2>Moscow 119334</s2><s3>RUS</s3><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>11 aut.</sZ></fA14><fA20><s1>318-328</s1></fA20><fA21><s1>2013</s1></fA21><fA23 i1="01"><s0>ENG</s0></fA23><fA43 i1="01"><s1>INIST</s1><s2>18839</s2><s5>354000503745790140</s5></fA43><fA44><s0>0000</s0><s1>© 2013 INIST-CNRS. All rights reserved.</s1></fA44><fA45><s0>1 p.1/4</s0></fA45><fA47 i1="01" i2="1"><s0>13-0152042</s0></fA47><fA60><s1>P</s1></fA60><fA61><s0>A</s0></fA61><fA64 i1="01" i2="1"><s0>Antiviral research</s0></fA64><fA66 i1="01"><s0>GBR</s0></fA66><fC01 i1="01" l="ENG"><s0>One effective method for the prevention and treatment of influenza infection is passive immunization. In our study, we examined the feasibility of creating an antibody-based preparation with a prolonged protective effect against influenza virus. Single-domain antibodies (sdAbs) specific for influenza virus hem-agglutinin were generated. Experiments in mouse models showed 100% survivability for both intranasal sdAbs administration 24 h prior to influenza challenge and 24 h after infection. sdAb-gene delivery by an adenoviral vector led to gene expression for up to 14 days. Protection by a recombinant adenovirus containing the sdAb gene was observed in cases of administration prior to influenza infection (14 d-24 h). We also demonstrated that the single administration of a combined preparation containing sdAb DNA and protein expanded the protection time window from 14 d prior to 48 h after influenza infection. This approach and the application of a broad-spectrum sdAbs will allow the development of efficient drugs for the prevention and treatment of viral infections produced by pandemic virus variants and other infections.</s0></fC01><fC02 i1="01" i2="X"><s0>002B02S05</s0></fC02><fC02 i1="02" i2="X"><s0>002B05C02C</s0></fC02><fC03 i1="01" i2="X" l="FRE"><s0>Immunisation passive</s0><s5>01</s5></fC03><fC03 i1="01" i2="X" l="ENG"><s0>Passive immunization</s0><s5>01</s5></fC03><fC03 i1="01" i2="X" l="SPA"><s0>Inmunización pasiva</s0><s5>01</s5></fC03><fC03 i1="02" i2="X" l="FRE"><s0>Adenoviridae</s0><s2>NW</s2><s5>02</s5></fC03><fC03 i1="02" i2="X" l="ENG"><s0>Adenoviridae</s0><s2>NW</s2><s5>02</s5></fC03><fC03 i1="02" i2="X" l="SPA"><s0>Adenoviridae</s0><s2>NW</s2><s5>02</s5></fC03><fC03 i1="03" i2="X" l="FRE"><s0>Adénovirus humain</s0><s2>NW</s2><s5>03</s5></fC03><fC03 i1="03" i2="X" l="ENG"><s0>Human adenovirus</s0><s2>NW</s2><s5>03</s5></fC03><fC03 i1="03" i2="X" l="SPA"><s0>Human adenovirus</s0><s2>NW</s2><s5>03</s5></fC03><fC03 i1="04" i2="X" l="FRE"><s0>Anticorps</s0><s5>04</s5></fC03><fC03 i1="04" i2="X" l="ENG"><s0>Antibody</s0><s5>04</s5></fC03><fC03 i1="04" i2="X" l="SPA"><s0>Anticuerpo</s0><s5>04</s5></fC03><fC03 i1="05" i2="X" l="FRE"><s0>Prévention</s0><s5>05</s5></fC03><fC03 i1="05" i2="X" l="ENG"><s0>Prevention</s0><s5>05</s5></fC03><fC03 i1="05" i2="X" l="SPA"><s0>Prevención</s0><s5>05</s5></fC03><fC03 i1="06" i2="X" l="FRE"><s0>Animal</s0><s5>06</s5></fC03><fC03 i1="06" i2="X" l="ENG"><s0>Animal</s0><s5>06</s5></fC03><fC03 i1="06" i2="X" l="SPA"><s0>Animal</s0><s5>06</s5></fC03><fC03 i1="07" i2="X" l="FRE"><s0>Souris</s0><s5>07</s5></fC03><fC03 i1="07" i2="X" l="ENG"><s0>Mouse</s0><s5>07</s5></fC03><fC03 i1="07" i2="X" l="SPA"><s0>Ratón</s0><s5>07</s5></fC03><fC03 i1="08" i2="X" l="FRE"><s0>Grippe</s0><s5>08</s5></fC03><fC03 i1="08" i2="X" l="ENG"><s0>Influenza</s0><s5>08</s5></fC03><fC03 i1="08" i2="X" l="SPA"><s0>Gripe</s0><s5>08</s5></fC03><fC03 i1="09" i2="X" l="FRE"><s0>Influenzavirus</s0><s2>NW</s2><s5>10</s5></fC03><fC03 i1="09" i2="X" l="ENG"><s0>Influenzavirus</s0><s2>NW</s2><s5>10</s5></fC03><fC03 i1="09" i2="X" l="SPA"><s0>Influenzavirus</s0><s2>NW</s2><s5>10</s5></fC03><fC03 i1="10" i2="X" l="FRE"><s0>Vecteur</s0><s5>11</s5></fC03><fC03 i1="10" i2="X" l="ENG"><s0>Vector</s0><s5>11</s5></fC03><fC03 i1="10" i2="X" l="SPA"><s0>Vector</s0><s5>11</s5></fC03><fC03 i1="11" i2="X" l="FRE"><s0>Forme fatale</s0><s4>INC</s4><s5>86</s5></fC03><fC07 i1="01" i2="X" l="FRE"><s0>Virus</s0><s2>NW</s2></fC07><fC07 i1="01" i2="X" l="ENG"><s0>Virus</s0><s2>NW</s2></fC07><fC07 i1="01" i2="X" l="SPA"><s0>Virus</s0><s2>NW</s2></fC07><fC07 i1="02" i2="X" l="FRE"><s0>Mastadenovirus</s0><s2>NW</s2></fC07><fC07 i1="02" i2="X" l="ENG"><s0>Mastadenovirus</s0><s2>NW</s2></fC07><fC07 i1="02" i2="X" l="SPA"><s0>Mastadenovirus</s0><s2>NW</s2></fC07><fC07 i1="03" i2="X" l="FRE"><s0>Rodentia</s0><s2>NS</s2></fC07><fC07 i1="03" i2="X" l="ENG"><s0>Rodentia</s0><s2>NS</s2></fC07><fC07 i1="03" i2="X" l="SPA"><s0>Rodentia</s0><s2>NS</s2></fC07><fC07 i1="04" i2="X" l="FRE"><s0>Mammalia</s0><s2>NS</s2></fC07><fC07 i1="04" i2="X" l="ENG"><s0>Mammalia</s0><s2>NS</s2></fC07><fC07 i1="04" i2="X" l="SPA"><s0>Mammalia</s0><s2>NS</s2></fC07><fC07 i1="05" i2="X" l="FRE"><s0>Vertebrata</s0><s2>NS</s2></fC07><fC07 i1="05" i2="X" l="ENG"><s0>Vertebrata</s0><s2>NS</s2></fC07><fC07 i1="05" i2="X" l="SPA"><s0>Vertebrata</s0><s2>NS</s2></fC07><fC07 i1="06" i2="X" l="FRE"><s0>Virose</s0></fC07><fC07 i1="06" i2="X" l="ENG"><s0>Viral disease</s0></fC07><fC07 i1="06" i2="X" l="SPA"><s0>Virosis</s0></fC07><fC07 i1="07" i2="X" l="FRE"><s0>Infection</s0></fC07><fC07 i1="07" i2="X" l="ENG"><s0>Infection</s0></fC07><fC07 i1="07" i2="X" l="SPA"><s0>Infección</s0></fC07><fC07 i1="08" i2="X" l="FRE"><s0>Orthomyxoviridae</s0><s2>NW</s2></fC07><fC07 i1="08" i2="X" l="ENG"><s0>Orthomyxoviridae</s0><s2>NW</s2></fC07><fC07 i1="08" i2="X" l="SPA"><s0>Orthomyxoviridae</s0><s2>NW</s2></fC07><fN21><s1>125</s1></fN21><fN44 i1="01"><s1>OTO</s1></fN44><fN82><s1>OTO</s1></fN82></pA></standard></inist><affiliations><list><country><li>Russie</li></country><region><li>District fédéral central</li></region><settlement><li>Moscou</li></settlement></list><tree><country name="Russie"><region name="District fédéral central"><name sortKey="Tutykhina, Irina L" sort="Tutykhina, Irina L" uniqKey="Tutykhina I" first="Irina L." last="Tutykhina">Irina L. Tutykhina</name></region><name sortKey="Gintsburg, Alexander L" sort="Gintsburg, Alexander L" uniqKey="Gintsburg A" first="Alexander L." last="Gintsburg">Alexander L. Gintsburg</name><name sortKey="Gribova, Irina Y" sort="Gribova, Irina Y" uniqKey="Gribova I" first="Irina Y." last="Gribova">Irina Y. Gribova</name><name sortKey="Ivanova, Tatiana I" sort="Ivanova, Tatiana I" uniqKey="Ivanova T" first="Tatiana I." last="Ivanova">Tatiana I. Ivanova</name><name sortKey="Logunov, Denis Y" sort="Logunov, Denis Y" uniqKey="Logunov D" first="Denis Y." last="Logunov">Denis Y. Logunov</name><name sortKey="Lysenko, Andrei A" sort="Lysenko, Andrei A" uniqKey="Lysenko A" first="Andrei A." last="Lysenko">Andrei A. Lysenko</name><name sortKey="Naroditsky, Boris S" sort="Naroditsky, Boris S" uniqKey="Naroditsky B" first="Boris S." last="Naroditsky">Boris S. Naroditsky</name><name sortKey="Rutovskaya, Marina V" sort="Rutovskaya, Marina V" uniqKey="Rutovskaya M" first="Marina V." last="Rutovskaya">Marina V. Rutovskaya</name><name sortKey="Sedova, Elena S" sort="Sedova, Elena S" uniqKey="Sedova E" first="Elena S." last="Sedova">Elena S. Sedova</name><name sortKey="Shmarov, Maxim M" sort="Shmarov, Maxim M" uniqKey="Shmarov M" first="Maxim M." last="Shmarov">Maxim M. Shmarov</name><name sortKey="Tillib, Sergei V" sort="Tillib, Sergei V" uniqKey="Tillib S" first="Sergei V." last="Tillib">Sergei V. Tillib</name><name sortKey="Vasilev, Lev A" sort="Vasilev, Lev A" uniqKey="Vasilev L" first="Lev A." last="Vasilev">Lev A. Vasilev</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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|texte= Passive immunization with a recombinant adenovirus expressing an HA (H5)-specific single-domain antibody protects mice from lethal influenza infection
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