Plasmacytoid Dendritic Cells and CD8 T Cells From Pregnant Women Show Altered Phenotype and Function Following H1N1/09 Infection
Identifieur interne : 000213 ( PascalFrancis/Checkpoint ); précédent : 000212; suivant : 000214Plasmacytoid Dendritic Cells and CD8 T Cells From Pregnant Women Show Altered Phenotype and Function Following H1N1/09 Infection
Auteurs : Rebecca L. Vanders [Australie] ; Peter G. Gibson [Australie] ; Vanessa E. Murphy [Australie] ; Peter A. B. Wark [Australie]Source :
- The Journal of infectious diseases [ 0022-1899 ] ; 2013.
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Abstract
Background. Pregnant women are a high-risk group during influenza pandemics. In this study we determined whether plasmacytoid dendritic cells (pDCs) and CD8 T cells from pregnant women display altered activity following in vitro infection with 2009 pandemic swine influenza (H1N1/09). Methods. Peripheral blood mononuclear cells (PBMCs) were isolated from pregnant (n = 26) and nonpregnant (n = 28) women. DC subtypes were enumerated from PBMCs. PBMCs were infected with H1N1/09 and CD86, human leukocyte antigen-DR (HLA-DR), and programmed death ligand 1/2 (PDL1/2) measured on pDCs. PD receptor 1 (PD1) was measured on CD8 T cells. Interferon-alpha (IFN-α), interleukin-2 (IL-2), tumor necrosis factor-alpha (TNFα), and IFN-gamma were measured from culture supernatant. Results. pDC (ie, CD303+/CD1c- PBMCs) percentages were lower in pregnant compared with nonpregnant women (P < .05). Following H1N1/09 infection, pDCs from pregnant women showed higher expression of CD86 (P < .01), HLA-DR (P < .001), and PDL1 (P < .001) compared with nonpregnant women. Expression of PD1 on CD8 T cells was higher during pregnancy (P < .05). Following H1N1/09 infection, PBMCs from pregnant women displayed reduced IFN-a (P < .01), IL-2 (P < .01), and IFN-γ (P < .01) compared with nonpregnant women. Blocking PDL1 during H1N1/09 infection increased these cytokines during pregnancy (P < .05). Conclusion. Altered maternal cellular antiviral activity is implicated in the increased morbidity during pregnancy following influenza pandemics.
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">Plasmacytoid Dendritic Cells and CD8 T Cells From Pregnant Women Show Altered Phenotype and Function Following H1N1/09 Infection</title><author><name sortKey="Vanders, Rebecca L" sort="Vanders, Rebecca L" uniqKey="Vanders R" first="Rebecca L." last="Vanders">Rebecca L. Vanders</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Centre for Asthma and Respiratory Diseases, School of Medicine and Public Health, The University of Newcastle</s1><s3>AUS</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>Australie</country><wicri:noRegion>Centre for Asthma and Respiratory Diseases, School of Medicine and Public Health, The University of Newcastle</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Hunter Medical Research Institute, John Hunter Hospital</s1><s2>Newcastle</s2><s3>AUS</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ></inist:fA14><country>Australie</country><wicri:noRegion>Newcastle</wicri:noRegion></affiliation></author><author><name sortKey="Gibson, Peter G" sort="Gibson, Peter G" uniqKey="Gibson P" first="Peter G." last="Gibson">Peter G. Gibson</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Centre for Asthma and Respiratory Diseases, School of Medicine and Public Health, The University of Newcastle</s1><s3>AUS</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>Australie</country><wicri:noRegion>Centre for Asthma and Respiratory Diseases, School of Medicine and Public Health, The University of Newcastle</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Department of Respiratory and Sleep Medicine, John Hunter Hospital</s1><s2>Newcastle</s2><s3>AUS</s3><sZ>2 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>Australie</country><wicri:noRegion>Newcastle</wicri:noRegion></affiliation></author><author><name sortKey="Murphy, Vanessa E" sort="Murphy, Vanessa E" uniqKey="Murphy V" first="Vanessa E." last="Murphy">Vanessa E. Murphy</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Centre for Asthma and Respiratory Diseases, School of Medicine and Public Health, The University of Newcastle</s1><s3>AUS</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>Australie</country><wicri:noRegion>Centre for Asthma and Respiratory Diseases, School of Medicine and Public Health, The University of Newcastle</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Hunter Medical Research Institute, John Hunter Hospital</s1><s2>Newcastle</s2><s3>AUS</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ></inist:fA14><country>Australie</country><wicri:noRegion>Newcastle</wicri:noRegion></affiliation></author><author><name sortKey="Wark, Peter A B" sort="Wark, Peter A B" uniqKey="Wark P" first="Peter A. B." last="Wark">Peter A. B. Wark</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Centre for Asthma and Respiratory Diseases, School of Medicine and Public Health, The University of Newcastle</s1><s3>AUS</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>Australie</country><wicri:noRegion>Centre for Asthma and Respiratory Diseases, School of Medicine and Public Health, The University of Newcastle</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Department of Respiratory and Sleep Medicine, John Hunter Hospital</s1><s2>Newcastle</s2><s3>AUS</s3><sZ>2 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>Australie</country><wicri:noRegion>Newcastle</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">INIST</idno><idno type="inist">13-0310021</idno><date when="2013">2013</date><idno type="stanalyst">PASCAL 13-0310021 INIST</idno><idno type="RBID">Pascal:13-0310021</idno><idno type="wicri:Area/PascalFrancis/Corpus">000221</idno><idno type="wicri:Area/PascalFrancis/Curation">001C06</idno><idno type="wicri:Area/PascalFrancis/Checkpoint">000213</idno><idno type="wicri:explorRef" wicri:stream="PascalFrancis" wicri:step="Checkpoint">000213</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Plasmacytoid Dendritic Cells and CD8 T Cells From Pregnant Women Show Altered Phenotype and Function Following H1N1/09 Infection</title><author><name sortKey="Vanders, Rebecca L" sort="Vanders, Rebecca L" uniqKey="Vanders R" first="Rebecca L." last="Vanders">Rebecca L. Vanders</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Centre for Asthma and Respiratory Diseases, School of Medicine and Public Health, The University of Newcastle</s1><s3>AUS</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>Australie</country><wicri:noRegion>Centre for Asthma and Respiratory Diseases, School of Medicine and Public Health, The University of Newcastle</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Hunter Medical Research Institute, John Hunter Hospital</s1><s2>Newcastle</s2><s3>AUS</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ></inist:fA14><country>Australie</country><wicri:noRegion>Newcastle</wicri:noRegion></affiliation></author><author><name sortKey="Gibson, Peter G" sort="Gibson, Peter G" uniqKey="Gibson P" first="Peter G." last="Gibson">Peter G. Gibson</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Centre for Asthma and Respiratory Diseases, School of Medicine and Public Health, The University of Newcastle</s1><s3>AUS</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>Australie</country><wicri:noRegion>Centre for Asthma and Respiratory Diseases, School of Medicine and Public Health, The University of Newcastle</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Department of Respiratory and Sleep Medicine, John Hunter Hospital</s1><s2>Newcastle</s2><s3>AUS</s3><sZ>2 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>Australie</country><wicri:noRegion>Newcastle</wicri:noRegion></affiliation></author><author><name sortKey="Murphy, Vanessa E" sort="Murphy, Vanessa E" uniqKey="Murphy V" first="Vanessa E." last="Murphy">Vanessa E. Murphy</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Centre for Asthma and Respiratory Diseases, School of Medicine and Public Health, The University of Newcastle</s1><s3>AUS</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>Australie</country><wicri:noRegion>Centre for Asthma and Respiratory Diseases, School of Medicine and Public Health, The University of Newcastle</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Hunter Medical Research Institute, John Hunter Hospital</s1><s2>Newcastle</s2><s3>AUS</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ></inist:fA14><country>Australie</country><wicri:noRegion>Newcastle</wicri:noRegion></affiliation></author><author><name sortKey="Wark, Peter A B" sort="Wark, Peter A B" uniqKey="Wark P" first="Peter A. B." last="Wark">Peter A. B. Wark</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Centre for Asthma and Respiratory Diseases, School of Medicine and Public Health, The University of Newcastle</s1><s3>AUS</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>Australie</country><wicri:noRegion>Centre for Asthma and Respiratory Diseases, School of Medicine and Public Health, The University of Newcastle</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Department of Respiratory and Sleep Medicine, John Hunter Hospital</s1><s2>Newcastle</s2><s3>AUS</s3><sZ>2 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>Australie</country><wicri:noRegion>Newcastle</wicri:noRegion></affiliation></author></analytic><series><title level="j" type="main">The Journal of infectious diseases</title><title level="j" type="abbreviated">J. infect. dis.</title><idno type="ISSN">0022-1899</idno><imprint><date when="2013">2013</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">The Journal of infectious diseases</title><title level="j" type="abbreviated">J. infect. dis.</title><idno type="ISSN">0022-1899</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>CD8 T lymphocyte</term><term>Dendritic cell</term><term>Female</term><term>Human</term><term>Infection</term><term>Influenzavirus A(H1N1)</term><term>Phenotype</term><term>Pregnancy</term><term>Woman</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Homme</term><term>Cellule dendritique</term><term>Lymphocyte T CD8</term><term>Gestation</term><term>Femelle</term><term>Femme</term><term>Phénotype</term><term>Infection</term><term>Virus grippal A(H1N1)</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Homme</term><term>Femme</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Background. Pregnant women are a high-risk group during influenza pandemics. In this study we determined whether plasmacytoid dendritic cells (pDCs) and CD8 T cells from pregnant women display altered activity following in vitro infection with 2009 pandemic swine influenza (H1N1/09). Methods. Peripheral blood mononuclear cells (PBMCs) were isolated from pregnant (n = 26) and nonpregnant (n =<sup> </sup>28) women. DC subtypes were enumerated from PBMCs. PBMCs were infected with H1N1/09 and CD86, human leukocyte antigen-DR (HLA-DR), and programmed death ligand 1/2 (PDL1/2) measured on pDCs. PD receptor 1 (PD1) was measured on CD8 T cells. Interferon-alpha (IFN-α), interleukin-2 (IL-2), tumor necrosis factor-alpha (TNFα), and IFN-gamma were measured from culture supernatant. Results. pDC (ie, CD303<sup>+</sup>/CD1c<sup>-</sup> PBMCs) percentages were lower in pregnant compared with nonpregnant women (P < .05). Following H1N1/09 infection, pDCs from pregnant women showed higher expression of CD86 (P < .01), HLA-DR (P < .001), and PDL1 (P < .001) compared with nonpregnant women. Expression of PD1 on CD8 T cells was higher during pregnancy (P < .05). Following H1N1/09 infection, PBMCs from pregnant women displayed reduced IFN-a (P < .01), IL-2 (P < .01), and IFN-γ (P < .01) compared with nonpregnant women. Blocking PDL1 during H1N1/09 infection increased these cytokines during pregnancy (P < .05). Conclusion. Altered maternal cellular antiviral activity is implicated in the increased morbidity during pregnancy following influenza pandemics.</div></front></TEI><inist><standard h6="B"><pA><fA01 i1="01" i2="1"><s0>0022-1899</s0></fA01><fA02 i1="01"><s0>JIDIAQ</s0></fA02><fA03 i2="1"><s0>J. infect. dis.</s0></fA03><fA05><s2>208</s2></fA05><fA06><s2>7</s2></fA06><fA08 i1="01" i2="1" l="ENG"><s1>Plasmacytoid Dendritic Cells and CD8 T Cells From Pregnant Women Show Altered Phenotype and Function Following H1N1/09 Infection</s1></fA08><fA11 i1="01" i2="1"><s1>VANDERS (Rebecca L.)</s1></fA11><fA11 i1="02" i2="1"><s1>GIBSON (Peter G.)</s1></fA11><fA11 i1="03" i2="1"><s1>MURPHY (Vanessa E.)</s1></fA11><fA11 i1="04" i2="1"><s1>WARK (Peter A. B.)</s1></fA11><fA14 i1="01"><s1>Centre for Asthma and Respiratory Diseases, School of Medicine and Public Health, The University of Newcastle</s1><s3>AUS</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ></fA14><fA14 i1="02"><s1>Hunter Medical Research Institute, John Hunter Hospital</s1><s2>Newcastle</s2><s3>AUS</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ></fA14><fA14 i1="03"><s1>Department of Respiratory and Sleep Medicine, John Hunter Hospital</s1><s2>Newcastle</s2><s3>AUS</s3><sZ>2 aut.</sZ><sZ>4 aut.</sZ></fA14><fA20><s1>1062-1070</s1></fA20><fA21><s1>2013</s1></fA21><fA23 i1="01"><s0>ENG</s0></fA23><fA43 i1="01"><s1>INIST</s1><s2>2052</s2><s5>354000505852320040</s5></fA43><fA44><s0>0000</s0><s1>© 2013 INIST-CNRS. All rights reserved.</s1></fA44><fA45><s0>42 ref.</s0></fA45><fA47 i1="01" i2="1"><s0>13-0310021</s0></fA47><fA60><s1>P</s1></fA60><fA61><s0>A</s0></fA61><fA64 i1="01" i2="1"><s0>The Journal of infectious diseases</s0></fA64><fA66 i1="01"><s0>GBR</s0></fA66><fC01 i1="01" l="ENG"><s0>Background. Pregnant women are a high-risk group during influenza pandemics. In this study we determined whether plasmacytoid dendritic cells (pDCs) and CD8 T cells from pregnant women display altered activity following in vitro infection with 2009 pandemic swine influenza (H1N1/09). Methods. Peripheral blood mononuclear cells (PBMCs) were isolated from pregnant (n = 26) and nonpregnant (n =<sup> </sup>28) women. DC subtypes were enumerated from PBMCs. PBMCs were infected with H1N1/09 and CD86, human leukocyte antigen-DR (HLA-DR), and programmed death ligand 1/2 (PDL1/2) measured on pDCs. PD receptor 1 (PD1) was measured on CD8 T cells. Interferon-alpha (IFN-α), interleukin-2 (IL-2), tumor necrosis factor-alpha (TNFα), and IFN-gamma were measured from culture supernatant. Results. pDC (ie, CD303<sup>+</sup>/CD1c<sup>-</sup> PBMCs) percentages were lower in pregnant compared with nonpregnant women (P < .05). Following H1N1/09 infection, pDCs from pregnant women showed higher expression of CD86 (P < .01), HLA-DR (P < .001), and PDL1 (P < .001) compared with nonpregnant women. Expression of PD1 on CD8 T cells was higher during pregnancy (P < .05). Following H1N1/09 infection, PBMCs from pregnant women displayed reduced IFN-a (P < .01), IL-2 (P < .01), and IFN-γ (P < .01) compared with nonpregnant women. Blocking PDL1 during H1N1/09 infection increased these cytokines during pregnancy (P < .05). Conclusion. Altered maternal cellular antiviral activity is implicated in the increased morbidity during pregnancy following influenza pandemics.</s0></fC01><fC02 i1="01" i2="X"><s0>002A05</s0></fC02><fC02 i1="02" i2="X"><s0>002B05</s0></fC02><fC03 i1="01" i2="X" l="FRE"><s0>Homme</s0><s5>01</s5></fC03><fC03 i1="01" i2="X" l="ENG"><s0>Human</s0><s5>01</s5></fC03><fC03 i1="01" i2="X" l="SPA"><s0>Hombre</s0><s5>01</s5></fC03><fC03 i1="02" i2="X" l="FRE"><s0>Cellule dendritique</s0><s5>05</s5></fC03><fC03 i1="02" i2="X" l="ENG"><s0>Dendritic cell</s0><s5>05</s5></fC03><fC03 i1="02" i2="X" l="SPA"><s0>Célula dendrítica</s0><s5>05</s5></fC03><fC03 i1="03" i2="X" l="FRE"><s0>Lymphocyte T CD8</s0><s5>06</s5></fC03><fC03 i1="03" i2="X" l="ENG"><s0>CD8 T lymphocyte</s0><s5>06</s5></fC03><fC03 i1="03" i2="X" l="SPA"><s0>Linfocito T CD8</s0><s5>06</s5></fC03><fC03 i1="04" i2="X" l="FRE"><s0>Gestation</s0><s5>07</s5></fC03><fC03 i1="04" i2="X" l="ENG"><s0>Pregnancy</s0><s5>07</s5></fC03><fC03 i1="04" i2="X" l="SPA"><s0>Gestación</s0><s5>07</s5></fC03><fC03 i1="05" i2="X" l="FRE"><s0>Femelle</s0><s5>08</s5></fC03><fC03 i1="05" i2="X" l="ENG"><s0>Female</s0><s5>08</s5></fC03><fC03 i1="05" i2="X" l="SPA"><s0>Hembra</s0><s5>08</s5></fC03><fC03 i1="06" i2="X" l="FRE"><s0>Femme</s0><s5>09</s5></fC03><fC03 i1="06" i2="X" l="ENG"><s0>Woman</s0><s5>09</s5></fC03><fC03 i1="06" i2="X" l="SPA"><s0>Mujer</s0><s5>09</s5></fC03><fC03 i1="07" i2="X" l="FRE"><s0>Phénotype</s0><s5>10</s5></fC03><fC03 i1="07" i2="X" l="ENG"><s0>Phenotype</s0><s5>10</s5></fC03><fC03 i1="07" i2="X" l="SPA"><s0>Fenotipo</s0><s5>10</s5></fC03><fC03 i1="08" i2="X" l="FRE"><s0>Infection</s0><s5>11</s5></fC03><fC03 i1="08" i2="X" l="ENG"><s0>Infection</s0><s5>11</s5></fC03><fC03 i1="08" i2="X" l="SPA"><s0>Infección</s0><s5>11</s5></fC03><fC03 i1="09" i2="X" l="FRE"><s0>Virus grippal A(H1N1)</s0><s4>CD</s4><s5>96</s5></fC03><fC03 i1="09" i2="X" l="ENG"><s0>Influenzavirus A(H1N1)</s0><s4>CD</s4><s5>96</s5></fC03><fN21><s1>294</s1></fN21><fN44 i1="01"><s1>OTO</s1></fN44><fN82><s1>OTO</s1></fN82></pA></standard></inist><affiliations><list><country><li>Australie</li></country></list><tree><country name="Australie"><noRegion><name sortKey="Vanders, Rebecca L" sort="Vanders, Rebecca L" uniqKey="Vanders R" first="Rebecca L." last="Vanders">Rebecca L. Vanders</name></noRegion><name sortKey="Gibson, Peter G" sort="Gibson, Peter G" uniqKey="Gibson P" first="Peter G." last="Gibson">Peter G. Gibson</name><name sortKey="Gibson, Peter G" sort="Gibson, Peter G" uniqKey="Gibson P" first="Peter G." last="Gibson">Peter G. Gibson</name><name sortKey="Murphy, Vanessa E" sort="Murphy, Vanessa E" uniqKey="Murphy V" first="Vanessa E." last="Murphy">Vanessa E. Murphy</name><name sortKey="Murphy, Vanessa E" sort="Murphy, Vanessa E" uniqKey="Murphy V" first="Vanessa E." last="Murphy">Vanessa E. Murphy</name><name sortKey="Vanders, Rebecca L" sort="Vanders, Rebecca L" uniqKey="Vanders R" first="Rebecca L." last="Vanders">Rebecca L. Vanders</name><name sortKey="Wark, Peter A B" sort="Wark, Peter A B" uniqKey="Wark P" first="Peter A. B." last="Wark">Peter A. B. Wark</name><name sortKey="Wark, Peter A B" sort="Wark, Peter A B" uniqKey="Wark P" first="Peter A. B." last="Wark">Peter A. B. 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