Process development for pandemic influenza VLP vaccine production using a baculovirus expression system
Identifieur interne : 002016 ( Ncbi/Merge ); précédent : 002015; suivant : 002017Process development for pandemic influenza VLP vaccine production using a baculovirus expression system
Auteurs : Chia-Chun Lai [Taïwan] ; Yu-Chieh Cheng [Taïwan] ; Pin-Wen Chen [Taïwan] ; Ting-Hui Lin [Taïwan] ; Tsai-Teng Tzeng [Taïwan] ; Chia-Chun Lu [Taïwan] ; Min-Shi Lee [Taïwan] ; Alan Yung-Chih Hu [Taïwan]Source :
- Journal of Biological Engineering [ 1754-1611 ] ; 2019.
Abstract
Influenza viruses cause hundreds of thousands of respiratory diseases worldwide each year, and vaccination is considered the most effective approach for preventing influenza annual epidemics or pandemics. Since 1950, chicken embryonated eggs have been used as the main method for producing seasonal influenza vaccines. However, this platform has the main drawback of a lack of scale-up flexibility, and thus, egg-based vaccine manufacturers cannot supply sufficient doses within a short period for use for pandemic prevention. As a result, strategies for reducing the manufacturing time and increasing production capacity are urgently needed. Non-virion vaccine methods have been considered an alternative strategy against an influenza pandemic, and the purpose of maintaining an immunogenic capsule structure with infectious properties appears to be met by the virus-like particle (VLP) platform.
An influenza H7N9-TW VLP production platform using insect cells, which included the expression of hemagglutinin (HA), NA, and M1 proteins, was established. To scale up H7N9-TW VLP production, several culture conditions were optimized to obtain a higher production yield. A high level of dissolved
In this study, a multigene expression platform and an effective upstream process were developed. Notably, a high H7N9-TW VLP yield was achieved using a two-step production strategy while a high DO level was maintained. The upstream process, which resulted in high VLP titers, could be further used for large-scale influenza VLP vaccine production.
Url:
DOI: 10.1186/s13036-019-0206-z
PubMed: 31666806
PubMed Central: 6813129
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<front><div type="abstract" xml:lang="en"><sec><title>Background</title>
<p id="Par1">Influenza viruses cause hundreds of thousands of respiratory diseases worldwide each year, and vaccination is considered the most effective approach for preventing influenza annual epidemics or pandemics. Since 1950, chicken embryonated eggs have been used as the main method for producing seasonal influenza vaccines. However, this platform has the main drawback of a lack of scale-up flexibility, and thus, egg-based vaccine manufacturers cannot supply sufficient doses within a short period for use for pandemic prevention. As a result, strategies for reducing the manufacturing time and increasing production capacity are urgently needed. Non-virion vaccine methods have been considered an alternative strategy against an influenza pandemic, and the purpose of maintaining an immunogenic capsule structure with infectious properties appears to be met by the virus-like particle (VLP) platform.</p>
</sec>
<sec><title>Results</title>
<p id="Par2">An influenza H7N9-TW VLP production platform using insect cells, which included the expression of hemagglutinin (HA), NA, and M1 proteins, was established. To scale up H7N9-TW VLP production, several culture conditions were optimized to obtain a higher production yield. A high level of dissolved <italic>oxygen (DO)</italic>
could be critical to H7N9-TW VLP production. If the DO was maintained at a high level, the HA titer obtained in the spinner flask system with ventilation was similar to that obtained in a shake flask. In this study, the HA titer in a 5-L bioreactor with a well-controlled DO level was substantially improved by 128-fold (from 4 HA units (HAU)/50 μL to 512 HAU/50 μL).</p>
</sec>
<sec><title>Conclusions</title>
<p id="Par3">In this study, a multigene expression platform and an effective upstream process were developed. Notably, a high H7N9-TW VLP yield was achieved using a two-step production strategy while a high DO level was maintained. The upstream process, which resulted in high VLP titers, could be further used for large-scale influenza VLP vaccine production.</p>
</sec>
</div>
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<biblStruct><analytic><author><name sortKey="Lua, Lh" uniqKey="Lua L">LH Lua</name>
</author>
<author><name sortKey="Connors, Nk" uniqKey="Connors N">NK Connors</name>
</author>
<author><name sortKey="Sainsbury, F" uniqKey="Sainsbury F">F Sainsbury</name>
</author>
<author><name sortKey="Chuan, Yp" uniqKey="Chuan Y">YP Chuan</name>
</author>
<author><name sortKey="Wibowo, N" uniqKey="Wibowo N">N Wibowo</name>
</author>
<author><name sortKey="Middelberg, Ap" uniqKey="Middelberg A">AP Middelberg</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Roldao, A" uniqKey="Roldao A">A Roldao</name>
</author>
<author><name sortKey="Mellado, Mc" uniqKey="Mellado M">MC Mellado</name>
</author>
<author><name sortKey="Castilho, Lr" uniqKey="Castilho L">LR Castilho</name>
</author>
<author><name sortKey="Carrondo, Mj" uniqKey="Carrondo M">MJ Carrondo</name>
</author>
<author><name sortKey="Alves, Pm" uniqKey="Alves P">PM Alves</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Frietze, Km" uniqKey="Frietze K">KM Frietze</name>
</author>
<author><name sortKey="Peabody, Ds" uniqKey="Peabody D">DS Peabody</name>
</author>
<author><name sortKey="Chackerian, B" uniqKey="Chackerian B">B Chackerian</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Liu, F" uniqKey="Liu F">F Liu</name>
</author>
<author><name sortKey="Wu, X" uniqKey="Wu X">X Wu</name>
</author>
<author><name sortKey="Li, L" uniqKey="Li L">L Li</name>
</author>
<author><name sortKey="Liu, Z" uniqKey="Liu Z">Z Liu</name>
</author>
<author><name sortKey="Wang, Z" uniqKey="Wang Z">Z Wang</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Thompson, Cm" uniqKey="Thompson C">CM Thompson</name>
</author>
<author><name sortKey="Aucoin, Mg" uniqKey="Aucoin M">MG Aucoin</name>
</author>
<author><name sortKey="Kamen, Aa" uniqKey="Kamen A">AA Kamen</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Effio, Cl" uniqKey="Effio C">CL Effio</name>
</author>
<author><name sortKey="Hubbuch, J" uniqKey="Hubbuch J">J Hubbuch</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Huang, X" uniqKey="Huang X">X Huang</name>
</author>
<author><name sortKey="Wang, X" uniqKey="Wang X">X Wang</name>
</author>
<author><name sortKey="Zhang, J" uniqKey="Zhang J">J Zhang</name>
</author>
<author><name sortKey="Xia, N" uniqKey="Xia N">N Xia</name>
</author>
<author><name sortKey="Zhao, Q" uniqKey="Zhao Q">Q Zhao</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Fuenmayor, J" uniqKey="Fuenmayor J">J Fuenmayor</name>
</author>
<author><name sortKey="Godia, F" uniqKey="Godia F">F Godia</name>
</author>
<author><name sortKey="Cervera, L" uniqKey="Cervera L">L Cervera</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Yamaji, H" uniqKey="Yamaji H">H Yamaji</name>
</author>
<author><name sortKey="Konishi, E" uniqKey="Konishi E">E Konishi</name>
</author>
</analytic>
</biblStruct>
<biblStruct></biblStruct>
<biblStruct><analytic><author><name sortKey="Wickham, Tjdt" uniqKey="Wickham T">TJDT Wickham</name>
</author>
<author><name sortKey="Granados, Rr" uniqKey="Granados R">RR Granados</name>
</author>
<author><name sortKey="Shuler, Ml" uniqKey="Shuler M">ML Shuler</name>
</author>
<author><name sortKey="Wood, Ha" uniqKey="Wood H">HA Wood</name>
</author>
</analytic>
</biblStruct>
<biblStruct></biblStruct>
<biblStruct><analytic><author><name sortKey="Zhang, Yh" uniqKey="Zhang Y">YH Zhang</name>
</author>
<author><name sortKey="Enden, G" uniqKey="Enden G">G Enden</name>
</author>
<author><name sortKey="Merchuk, Jc" uniqKey="Merchuk J">JC Merchuk</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Thompson, Cm" uniqKey="Thompson C">CM Thompson</name>
</author>
<author><name sortKey="Petiot, E" uniqKey="Petiot E">E Petiot</name>
</author>
<author><name sortKey="Mullick, A" uniqKey="Mullick A">A Mullick</name>
</author>
<author><name sortKey="Aucoin, Mg" uniqKey="Aucoin M">MG Aucoin</name>
</author>
<author><name sortKey="Henry, O" uniqKey="Henry O">O Henry</name>
</author>
<author><name sortKey="Kamen, Aa" uniqKey="Kamen A">AA Kamen</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Lopez Vidal, J" uniqKey="Lopez Vidal J">J Lopez-Vidal</name>
</author>
<author><name sortKey="G Mez Sebastian, S" uniqKey="G Mez Sebastian S">S Gómez-Sebastián</name>
</author>
<author><name sortKey="Barcena, J" uniqKey="Barcena J">J Barcena</name>
</author>
<author><name sortKey="Nunez Mdel, C" uniqKey="Nunez Mdel C">C Nunez Mdel</name>
</author>
<author><name sortKey="Martinez Alonso, D" uniqKey="Martinez Alonso D">D Martinez-Alonso</name>
</author>
<author><name sortKey="Dudognon, B" uniqKey="Dudognon B">B Dudognon</name>
</author>
<author><name sortKey="Guijarro, E" uniqKey="Guijarro E">E Guijarro</name>
</author>
<author><name sortKey="Escribano, Jm" uniqKey="Escribano J">JM Escribano</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Krammer, F" uniqKey="Krammer F">F Krammer</name>
</author>
<author><name sortKey="Schinko, T" uniqKey="Schinko T">T Schinko</name>
</author>
<author><name sortKey="Palmberger, D" uniqKey="Palmberger D">D Palmberger</name>
</author>
<author><name sortKey="Tauer, C" uniqKey="Tauer C">C Tauer</name>
</author>
<author><name sortKey="Messner, P" uniqKey="Messner P">P Messner</name>
</author>
<author><name sortKey="Grabherr, R" uniqKey="Grabherr R">R Grabherr</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Ignacio Casal, J" uniqKey="Ignacio Casal J">J Ignacio Casal</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Chung, Cy" uniqKey="Chung C">CY Chung</name>
</author>
<author><name sortKey="Chen, Cy" uniqKey="Chen C">CY Chen</name>
</author>
<author><name sortKey="Lin, Sy" uniqKey="Lin S">SY Lin</name>
</author>
<author><name sortKey="Chung, Yc" uniqKey="Chung Y">YC Chung</name>
</author>
<author><name sortKey="Chiu, Hy" uniqKey="Chiu H">HY Chiu</name>
</author>
<author><name sortKey="Chi, Wk" uniqKey="Chi W">WK Chi</name>
</author>
<author><name sortKey="Lin, Yl" uniqKey="Lin Y">YL Lin</name>
</author>
<author><name sortKey="Chiang, Bl" uniqKey="Chiang B">BL Chiang</name>
</author>
<author><name sortKey="Chen, Wj" uniqKey="Chen W">WJ Chen</name>
</author>
<author><name sortKey="Hu, Yc" uniqKey="Hu Y">YC Hu</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Bornstein, J" uniqKey="Bornstein J">J Bornstein</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Wang, Jw" uniqKey="Wang J">JW Wang</name>
</author>
<author><name sortKey="Roden, Rb" uniqKey="Roden R">RB Roden</name>
</author>
</analytic>
</biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct><analytic><author><name sortKey="Mohsen, Mo" uniqKey="Mohsen M">MO Mohsen</name>
</author>
<author><name sortKey="Zha, L" uniqKey="Zha L">L Zha</name>
</author>
<author><name sortKey="Cabral Miranda, G" uniqKey="Cabral Miranda G">G Cabral-Miranda</name>
</author>
<author><name sortKey="Bachmann, Mf" uniqKey="Bachmann M">MF Bachmann</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Liu, Yv" uniqKey="Liu Y">YV Liu</name>
</author>
<author><name sortKey="Massare, Mj" uniqKey="Massare M">MJ Massare</name>
</author>
<author><name sortKey="Pearce, Mb" uniqKey="Pearce M">MB Pearce</name>
</author>
<author><name sortKey="Sun, X" uniqKey="Sun X">X Sun</name>
</author>
<author><name sortKey="Belser, Ja" uniqKey="Belser J">JA Belser</name>
</author>
<author><name sortKey="Maines, Tr" uniqKey="Maines T">TR Maines</name>
</author>
<author><name sortKey="Creager, Hm" uniqKey="Creager H">HM Creager</name>
</author>
<author><name sortKey="Glenn, Gm" uniqKey="Glenn G">GM Glenn</name>
</author>
<author><name sortKey="Pushko, P" uniqKey="Pushko P">P Pushko</name>
</author>
<author><name sortKey="Smith, Ge" uniqKey="Smith G">GE Smith</name>
</author>
<author><name sortKey="Tumpey, Tm" uniqKey="Tumpey T">TM Tumpey</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kushnir, N" uniqKey="Kushnir N">N Kushnir</name>
</author>
<author><name sortKey="Streatfield, Sj" uniqKey="Streatfield S">SJ Streatfield</name>
</author>
<author><name sortKey="Yusibov, V" uniqKey="Yusibov V">V Yusibov</name>
</author>
</analytic>
</biblStruct>
<biblStruct></biblStruct>
<biblStruct><analytic><author><name sortKey="Pushko, P" uniqKey="Pushko P">P Pushko</name>
</author>
<author><name sortKey="Kort, T" uniqKey="Kort T">T Kort</name>
</author>
<author><name sortKey="Nathan, M" uniqKey="Nathan M">M Nathan</name>
</author>
<author><name sortKey="Pearce, Mb" uniqKey="Pearce M">MB Pearce</name>
</author>
<author><name sortKey="Smith, G" uniqKey="Smith G">G Smith</name>
</author>
<author><name sortKey="Tumpey, Tm" uniqKey="Tumpey T">TM Tumpey</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Bright, Ra" uniqKey="Bright R">RA Bright</name>
</author>
<author><name sortKey="Carter, Dm" uniqKey="Carter D">DM Carter</name>
</author>
<author><name sortKey="Daniluk, S" uniqKey="Daniluk S">S Daniluk</name>
</author>
<author><name sortKey="Toapanta, Fr" uniqKey="Toapanta F">FR Toapanta</name>
</author>
<author><name sortKey="Ahmad, A" uniqKey="Ahmad A">A Ahmad</name>
</author>
<author><name sortKey="Gavrilov, V" uniqKey="Gavrilov V">V Gavrilov</name>
</author>
<author><name sortKey="Massare, M" uniqKey="Massare M">M Massare</name>
</author>
<author><name sortKey="Pushko, P" uniqKey="Pushko P">P Pushko</name>
</author>
<author><name sortKey="Mytle, N" uniqKey="Mytle N">N Mytle</name>
</author>
<author><name sortKey="Rowe, T" uniqKey="Rowe T">T Rowe</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Cox, Mm" uniqKey="Cox M">MM Cox</name>
</author>
<author><name sortKey="Patriarca, Pa" uniqKey="Patriarca P">PA Patriarca</name>
</author>
<author><name sortKey="Treanor, J" uniqKey="Treanor J">J Treanor</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Cox, Mmir" uniqKey="Cox M">MMIR Cox</name>
</author>
<author><name sortKey="Post, P" uniqKey="Post P">P Post</name>
</author>
<author><name sortKey="Dunkle, L" uniqKey="Dunkle L">L Dunkle</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Chang, S Y" uniqKey="Chang S">S-Y Chang</name>
</author>
<author><name sortKey="Lin, P H" uniqKey="Lin P">P-H Lin</name>
</author>
<author><name sortKey="Tsai, J C" uniqKey="Tsai J">J-C Tsai</name>
</author>
<author><name sortKey="Hung, C C" uniqKey="Hung C">C-C Hung</name>
</author>
<author><name sortKey="Chang, S C" uniqKey="Chang S">S-C Chang</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Yang, Jr" uniqKey="Yang J">JR Yang</name>
</author>
<author><name sortKey="Kuo, Cy" uniqKey="Kuo C">CY Kuo</name>
</author>
<author><name sortKey="Huang, Hy" uniqKey="Huang H">HY Huang</name>
</author>
<author><name sortKey="Wu, Ft" uniqKey="Wu F">FT Wu</name>
</author>
<author><name sortKey="Huang, Yl" uniqKey="Huang Y">YL Huang</name>
</author>
<author><name sortKey="Cheng, Cy" uniqKey="Cheng C">CY Cheng</name>
</author>
<author><name sortKey="Su, Yt" uniqKey="Su Y">YT Su</name>
</author>
<author><name sortKey="Wu, Hs" uniqKey="Wu H">HS Wu</name>
</author>
<author><name sortKey="Liu, Mt" uniqKey="Liu M">MT Liu</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Wu, Cy" uniqKey="Wu C">CY Wu</name>
</author>
<author><name sortKey="Yeh, Yc" uniqKey="Yeh Y">YC Yeh</name>
</author>
<author><name sortKey="Yang, Yc" uniqKey="Yang Y">YC Yang</name>
</author>
<author><name sortKey="Chou, C" uniqKey="Chou C">C Chou</name>
</author>
<author><name sortKey="Liu, Mt" uniqKey="Liu M">MT Liu</name>
</author>
<author><name sortKey="Wu, Hs" uniqKey="Wu H">HS Wu</name>
</author>
<author><name sortKey="Chan, Jt" uniqKey="Chan J">JT Chan</name>
</author>
<author><name sortKey="Hsiao, Pw" uniqKey="Hsiao P">PW Hsiao</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Smith, Ge" uniqKey="Smith G">GE Smith</name>
</author>
<author><name sortKey="Flyer, Dc" uniqKey="Flyer D">DC Flyer</name>
</author>
<author><name sortKey="Raghunandan, R" uniqKey="Raghunandan R">R Raghunandan</name>
</author>
<author><name sortKey="Liu, Y" uniqKey="Liu Y">Y Liu</name>
</author>
<author><name sortKey="Wei, Z" uniqKey="Wei Z">Z Wei</name>
</author>
<author><name sortKey="Wu, Y" uniqKey="Wu Y">Y Wu</name>
</author>
<author><name sortKey="Kpamegan, E" uniqKey="Kpamegan E">E Kpamegan</name>
</author>
<author><name sortKey="Courbron, D" uniqKey="Courbron D">D Courbron</name>
</author>
<author><name sortKey="Fries, Lf" uniqKey="Fries L">LF Fries</name>
</author>
<author><name sortKey="Glenn, Gm" uniqKey="Glenn G">GM Glenn</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Chia, My" uniqKey="Chia M">MY Chia</name>
</author>
<author><name sortKey="Hu, Ay" uniqKey="Hu A">AY Hu</name>
</author>
<author><name sortKey="Tseng, Yf" uniqKey="Tseng Y">YF Tseng</name>
</author>
<author><name sortKey="Weng, Tc" uniqKey="Weng T">TC Weng</name>
</author>
<author><name sortKey="Lai, Cc" uniqKey="Lai C">CC Lai</name>
</author>
<author><name sortKey="Lin, Jy" uniqKey="Lin J">JY Lin</name>
</author>
<author><name sortKey="Chen, Pl" uniqKey="Chen P">PL Chen</name>
</author>
<author><name sortKey="Wang, Yf" uniqKey="Wang Y">YF Wang</name>
</author>
<author><name sortKey="Chao, Sr" uniqKey="Chao S">SR Chao</name>
</author>
<author><name sortKey="Chang, Jy" uniqKey="Chang J">JY Chang</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Fernandes Platzgummer, A" uniqKey="Fernandes Platzgummer A">A Fernandes-Platzgummer</name>
</author>
<author><name sortKey="Diogo, Mm" uniqKey="Diogo M">MM Diogo</name>
</author>
<author><name sortKey="Baptista, Rp" uniqKey="Baptista R">RP Baptista</name>
</author>
<author><name sortKey="Da Silva, Cl" uniqKey="Da Silva C">CL da Silva</name>
</author>
<author><name sortKey="Cabral, Jm" uniqKey="Cabral J">JM Cabral</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Hu, Ay" uniqKey="Hu A">AY Hu</name>
</author>
<author><name sortKey="Tseng, Yf" uniqKey="Tseng Y">YF Tseng</name>
</author>
<author><name sortKey="Weng, Tc" uniqKey="Weng T">TC Weng</name>
</author>
<author><name sortKey="Liao, Cc" uniqKey="Liao C">CC Liao</name>
</author>
<author><name sortKey="Wu, J" uniqKey="Wu J">J Wu</name>
</author>
<author><name sortKey="Chou, Ah" uniqKey="Chou A">AH Chou</name>
</author>
<author><name sortKey="Chao, Hj" uniqKey="Chao H">HJ Chao</name>
</author>
<author><name sortKey="Gu, A" uniqKey="Gu A">A Gu</name>
</author>
<author><name sortKey="Chen, J" uniqKey="Chen J">J Chen</name>
</author>
<author><name sortKey="Lin, Sc" uniqKey="Lin S">SC Lin</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Hidalgo, D" uniqKey="Hidalgo D">D Hidalgo</name>
</author>
<author><name sortKey="Paz, E" uniqKey="Paz E">E Paz</name>
</author>
<author><name sortKey="Palomares, La" uniqKey="Palomares L">LA Palomares</name>
</author>
<author><name sortKey="Ramirez, Ot" uniqKey="Ramirez O">OT Ramirez</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Zhang, Fsm" uniqKey="Zhang F">FSM Zhang</name>
</author>
<author><name sortKey="Itle, Lj" uniqKey="Itle L">LJ Itle</name>
</author>
<author><name sortKey="Lang, Sc" uniqKey="Lang S">SC Lang</name>
</author>
<author><name sortKey="Murhammer, Dw" uniqKey="Murhammer D">DW Murhammer</name>
</author>
<author><name sortKey="Linhardt, Rj" uniqKey="Linhardt R">RJ Linhardt</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Quan, Fs" uniqKey="Quan F">FS Quan</name>
</author>
<author><name sortKey="Lee, Yt" uniqKey="Lee Y">YT Lee</name>
</author>
<author><name sortKey="Kim, Kh" uniqKey="Kim K">KH Kim</name>
</author>
<author><name sortKey="Kim, Mc" uniqKey="Kim M">MC Kim</name>
</author>
<author><name sortKey="Kang, Sm" uniqKey="Kang S">SM Kang</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Li, C" uniqKey="Li C">C Li</name>
</author>
<author><name sortKey="Xu, K" uniqKey="Xu K">K Xu</name>
</author>
<author><name sortKey="Hashem, A" uniqKey="Hashem A">A Hashem</name>
</author>
<author><name sortKey="Shao, M" uniqKey="Shao M">M Shao</name>
</author>
<author><name sortKey="Liu, S" uniqKey="Liu S">S Liu</name>
</author>
<author><name sortKey="Zou, Y" uniqKey="Zou Y">Y Zou</name>
</author>
<author><name sortKey="Gao, Q" uniqKey="Gao Q">Q Gao</name>
</author>
<author><name sortKey="Zhang, Y" uniqKey="Zhang Y">Y Zhang</name>
</author>
<author><name sortKey="Yuan, L" uniqKey="Yuan L">L Yuan</name>
</author>
<author><name sortKey="Xu, M" uniqKey="Xu M">M Xu</name>
</author>
</analytic>
</biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct><analytic><author><name sortKey="Genzel, Y" uniqKey="Genzel Y">Y Genzel</name>
</author>
<author><name sortKey="Behrendt, I" uniqKey="Behrendt I">I Behrendt</name>
</author>
<author><name sortKey="Rodig, J" uniqKey="Rodig J">J Rodig</name>
</author>
<author><name sortKey="Rapp, E" uniqKey="Rapp E">E Rapp</name>
</author>
<author><name sortKey="Kueppers, C" uniqKey="Kueppers C">C Kueppers</name>
</author>
<author><name sortKey="Kochanek, S" uniqKey="Kochanek S">S Kochanek</name>
</author>
<author><name sortKey="Schiedner, G" uniqKey="Schiedner G">G Schiedner</name>
</author>
<author><name sortKey="Reichl, U" uniqKey="Reichl U">U Reichl</name>
</author>
</analytic>
</biblStruct>
</listBibl>
</div1>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Process development for pandemic influenza VLP vaccine production using a baculovirus expression system</title>
<author><name sortKey="Lai, Chia Chun" sort="Lai, Chia Chun" uniqKey="Lai C" first="Chia-Chun" last="Lai">Chia-Chun Lai</name>
<affiliation wicri:level="1"><nlm:aff id="Aff1">National Institute of Infectious Diseases and Vaccinology, NHRI, 35 Keyan Road, Zhunan, Miaoli County, 35053 Taiwan</nlm:aff>
<country xml:lang="fr">Taïwan</country>
<wicri:regionArea>National Institute of Infectious Diseases and Vaccinology, NHRI, 35 Keyan Road, Zhunan, Miaoli County</wicri:regionArea>
<wicri:noRegion>Miaoli County</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="ISNI">0000 0004 0532 0580</institution-id>
<institution-id institution-id-type="GRID">grid.38348.34</institution-id>
<institution>College of Life Science,</institution>
<institution>National Tsing Hua University,</institution>
</institution-wrap>
101, Section 2, Kuang-Fu Road, Hsinchu, 30013 Taiwan</nlm:aff>
<country xml:lang="fr">Taïwan</country>
<wicri:regionArea>101, Section 2, Kuang-Fu Road, Hsinchu</wicri:regionArea>
<wicri:noRegion>Hsinchu</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Cheng, Yu Chieh" sort="Cheng, Yu Chieh" uniqKey="Cheng Y" first="Yu-Chieh" last="Cheng">Yu-Chieh Cheng</name>
<affiliation wicri:level="1"><nlm:aff id="Aff1">National Institute of Infectious Diseases and Vaccinology, NHRI, 35 Keyan Road, Zhunan, Miaoli County, 35053 Taiwan</nlm:aff>
<country xml:lang="fr">Taïwan</country>
<wicri:regionArea>National Institute of Infectious Diseases and Vaccinology, NHRI, 35 Keyan Road, Zhunan, Miaoli County</wicri:regionArea>
<wicri:noRegion>Miaoli County</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Chen, Pin Wen" sort="Chen, Pin Wen" uniqKey="Chen P" first="Pin-Wen" last="Chen">Pin-Wen Chen</name>
<affiliation wicri:level="1"><nlm:aff id="Aff1">National Institute of Infectious Diseases and Vaccinology, NHRI, 35 Keyan Road, Zhunan, Miaoli County, 35053 Taiwan</nlm:aff>
<country xml:lang="fr">Taïwan</country>
<wicri:regionArea>National Institute of Infectious Diseases and Vaccinology, NHRI, 35 Keyan Road, Zhunan, Miaoli County</wicri:regionArea>
<wicri:noRegion>Miaoli County</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Lin, Ting Hui" sort="Lin, Ting Hui" uniqKey="Lin T" first="Ting-Hui" last="Lin">Ting-Hui Lin</name>
<affiliation wicri:level="1"><nlm:aff id="Aff1">National Institute of Infectious Diseases and Vaccinology, NHRI, 35 Keyan Road, Zhunan, Miaoli County, 35053 Taiwan</nlm:aff>
<country xml:lang="fr">Taïwan</country>
<wicri:regionArea>National Institute of Infectious Diseases and Vaccinology, NHRI, 35 Keyan Road, Zhunan, Miaoli County</wicri:regionArea>
<wicri:noRegion>Miaoli County</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="ISNI">0000 0004 0532 0580</institution-id>
<institution-id institution-id-type="GRID">grid.38348.34</institution-id>
<institution>College of Life Science,</institution>
<institution>National Tsing Hua University,</institution>
</institution-wrap>
101, Section 2, Kuang-Fu Road, Hsinchu, 30013 Taiwan</nlm:aff>
<country xml:lang="fr">Taïwan</country>
<wicri:regionArea>101, Section 2, Kuang-Fu Road, Hsinchu</wicri:regionArea>
<wicri:noRegion>Hsinchu</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Tzeng, Tsai Teng" sort="Tzeng, Tsai Teng" uniqKey="Tzeng T" first="Tsai-Teng" last="Tzeng">Tsai-Teng Tzeng</name>
<affiliation wicri:level="1"><nlm:aff id="Aff1">National Institute of Infectious Diseases and Vaccinology, NHRI, 35 Keyan Road, Zhunan, Miaoli County, 35053 Taiwan</nlm:aff>
<country xml:lang="fr">Taïwan</country>
<wicri:regionArea>National Institute of Infectious Diseases and Vaccinology, NHRI, 35 Keyan Road, Zhunan, Miaoli County</wicri:regionArea>
<wicri:noRegion>Miaoli County</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Lu, Chia Chun" sort="Lu, Chia Chun" uniqKey="Lu C" first="Chia-Chun" last="Lu">Chia-Chun Lu</name>
<affiliation wicri:level="1"><nlm:aff id="Aff1">National Institute of Infectious Diseases and Vaccinology, NHRI, 35 Keyan Road, Zhunan, Miaoli County, 35053 Taiwan</nlm:aff>
<country xml:lang="fr">Taïwan</country>
<wicri:regionArea>National Institute of Infectious Diseases and Vaccinology, NHRI, 35 Keyan Road, Zhunan, Miaoli County</wicri:regionArea>
<wicri:noRegion>Miaoli County</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Lee, Min Shi" sort="Lee, Min Shi" uniqKey="Lee M" first="Min-Shi" last="Lee">Min-Shi Lee</name>
<affiliation wicri:level="1"><nlm:aff id="Aff1">National Institute of Infectious Diseases and Vaccinology, NHRI, 35 Keyan Road, Zhunan, Miaoli County, 35053 Taiwan</nlm:aff>
<country xml:lang="fr">Taïwan</country>
<wicri:regionArea>National Institute of Infectious Diseases and Vaccinology, NHRI, 35 Keyan Road, Zhunan, Miaoli County</wicri:regionArea>
<wicri:noRegion>Miaoli County</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Hu, Alan Yung Chih" sort="Hu, Alan Yung Chih" uniqKey="Hu A" first="Alan Yung-Chih" last="Hu">Alan Yung-Chih Hu</name>
<affiliation wicri:level="1"><nlm:aff id="Aff1">National Institute of Infectious Diseases and Vaccinology, NHRI, 35 Keyan Road, Zhunan, Miaoli County, 35053 Taiwan</nlm:aff>
<country xml:lang="fr">Taïwan</country>
<wicri:regionArea>National Institute of Infectious Diseases and Vaccinology, NHRI, 35 Keyan Road, Zhunan, Miaoli County</wicri:regionArea>
<wicri:noRegion>Miaoli County</wicri:noRegion>
</affiliation>
</author>
</analytic>
<series><title level="j">Journal of Biological Engineering</title>
<idno type="eISSN">1754-1611</idno>
<imprint><date when="2019">2019</date>
</imprint>
</series>
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<front><div type="abstract" xml:lang="en"><sec><title>Background</title>
<p id="Par1">Influenza viruses cause hundreds of thousands of respiratory diseases worldwide each year, and vaccination is considered the most effective approach for preventing influenza annual epidemics or pandemics. Since 1950, chicken embryonated eggs have been used as the main method for producing seasonal influenza vaccines. However, this platform has the main drawback of a lack of scale-up flexibility, and thus, egg-based vaccine manufacturers cannot supply sufficient doses within a short period for use for pandemic prevention. As a result, strategies for reducing the manufacturing time and increasing production capacity are urgently needed. Non-virion vaccine methods have been considered an alternative strategy against an influenza pandemic, and the purpose of maintaining an immunogenic capsule structure with infectious properties appears to be met by the virus-like particle (VLP) platform.</p>
</sec>
<sec><title>Results</title>
<p id="Par2">An influenza H7N9-TW VLP production platform using insect cells, which included the expression of hemagglutinin (HA), NA, and M1 proteins, was established. To scale up H7N9-TW VLP production, several culture conditions were optimized to obtain a higher production yield. A high level of dissolved <italic>oxygen (DO)</italic>
could be critical to H7N9-TW VLP production. If the DO was maintained at a high level, the HA titer obtained in the spinner flask system with ventilation was similar to that obtained in a shake flask. In this study, the HA titer in a 5-L bioreactor with a well-controlled DO level was substantially improved by 128-fold (from 4 HA units (HAU)/50 μL to 512 HAU/50 μL).</p>
</sec>
<sec><title>Conclusions</title>
<p id="Par3">In this study, a multigene expression platform and an effective upstream process were developed. Notably, a high H7N9-TW VLP yield was achieved using a two-step production strategy while a high DO level was maintained. The upstream process, which resulted in high VLP titers, could be further used for large-scale influenza VLP vaccine production.</p>
</sec>
</div>
</front>
<back><div1 type="bibliography"><listBibl><biblStruct></biblStruct>
<biblStruct><analytic><author><name sortKey="Lua, Lh" uniqKey="Lua L">LH Lua</name>
</author>
<author><name sortKey="Connors, Nk" uniqKey="Connors N">NK Connors</name>
</author>
<author><name sortKey="Sainsbury, F" uniqKey="Sainsbury F">F Sainsbury</name>
</author>
<author><name sortKey="Chuan, Yp" uniqKey="Chuan Y">YP Chuan</name>
</author>
<author><name sortKey="Wibowo, N" uniqKey="Wibowo N">N Wibowo</name>
</author>
<author><name sortKey="Middelberg, Ap" uniqKey="Middelberg A">AP Middelberg</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Roldao, A" uniqKey="Roldao A">A Roldao</name>
</author>
<author><name sortKey="Mellado, Mc" uniqKey="Mellado M">MC Mellado</name>
</author>
<author><name sortKey="Castilho, Lr" uniqKey="Castilho L">LR Castilho</name>
</author>
<author><name sortKey="Carrondo, Mj" uniqKey="Carrondo M">MJ Carrondo</name>
</author>
<author><name sortKey="Alves, Pm" uniqKey="Alves P">PM Alves</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Frietze, Km" uniqKey="Frietze K">KM Frietze</name>
</author>
<author><name sortKey="Peabody, Ds" uniqKey="Peabody D">DS Peabody</name>
</author>
<author><name sortKey="Chackerian, B" uniqKey="Chackerian B">B Chackerian</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Liu, F" uniqKey="Liu F">F Liu</name>
</author>
<author><name sortKey="Wu, X" uniqKey="Wu X">X Wu</name>
</author>
<author><name sortKey="Li, L" uniqKey="Li L">L Li</name>
</author>
<author><name sortKey="Liu, Z" uniqKey="Liu Z">Z Liu</name>
</author>
<author><name sortKey="Wang, Z" uniqKey="Wang Z">Z Wang</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Thompson, Cm" uniqKey="Thompson C">CM Thompson</name>
</author>
<author><name sortKey="Aucoin, Mg" uniqKey="Aucoin M">MG Aucoin</name>
</author>
<author><name sortKey="Kamen, Aa" uniqKey="Kamen A">AA Kamen</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Effio, Cl" uniqKey="Effio C">CL Effio</name>
</author>
<author><name sortKey="Hubbuch, J" uniqKey="Hubbuch J">J Hubbuch</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Huang, X" uniqKey="Huang X">X Huang</name>
</author>
<author><name sortKey="Wang, X" uniqKey="Wang X">X Wang</name>
</author>
<author><name sortKey="Zhang, J" uniqKey="Zhang J">J Zhang</name>
</author>
<author><name sortKey="Xia, N" uniqKey="Xia N">N Xia</name>
</author>
<author><name sortKey="Zhao, Q" uniqKey="Zhao Q">Q Zhao</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Fuenmayor, J" uniqKey="Fuenmayor J">J Fuenmayor</name>
</author>
<author><name sortKey="Godia, F" uniqKey="Godia F">F Godia</name>
</author>
<author><name sortKey="Cervera, L" uniqKey="Cervera L">L Cervera</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Yamaji, H" uniqKey="Yamaji H">H Yamaji</name>
</author>
<author><name sortKey="Konishi, E" uniqKey="Konishi E">E Konishi</name>
</author>
</analytic>
</biblStruct>
<biblStruct></biblStruct>
<biblStruct><analytic><author><name sortKey="Wickham, Tjdt" uniqKey="Wickham T">TJDT Wickham</name>
</author>
<author><name sortKey="Granados, Rr" uniqKey="Granados R">RR Granados</name>
</author>
<author><name sortKey="Shuler, Ml" uniqKey="Shuler M">ML Shuler</name>
</author>
<author><name sortKey="Wood, Ha" uniqKey="Wood H">HA Wood</name>
</author>
</analytic>
</biblStruct>
<biblStruct></biblStruct>
<biblStruct><analytic><author><name sortKey="Zhang, Yh" uniqKey="Zhang Y">YH Zhang</name>
</author>
<author><name sortKey="Enden, G" uniqKey="Enden G">G Enden</name>
</author>
<author><name sortKey="Merchuk, Jc" uniqKey="Merchuk J">JC Merchuk</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Thompson, Cm" uniqKey="Thompson C">CM Thompson</name>
</author>
<author><name sortKey="Petiot, E" uniqKey="Petiot E">E Petiot</name>
</author>
<author><name sortKey="Mullick, A" uniqKey="Mullick A">A Mullick</name>
</author>
<author><name sortKey="Aucoin, Mg" uniqKey="Aucoin M">MG Aucoin</name>
</author>
<author><name sortKey="Henry, O" uniqKey="Henry O">O Henry</name>
</author>
<author><name sortKey="Kamen, Aa" uniqKey="Kamen A">AA Kamen</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Lopez Vidal, J" uniqKey="Lopez Vidal J">J Lopez-Vidal</name>
</author>
<author><name sortKey="G Mez Sebastian, S" uniqKey="G Mez Sebastian S">S Gómez-Sebastián</name>
</author>
<author><name sortKey="Barcena, J" uniqKey="Barcena J">J Barcena</name>
</author>
<author><name sortKey="Nunez Mdel, C" uniqKey="Nunez Mdel C">C Nunez Mdel</name>
</author>
<author><name sortKey="Martinez Alonso, D" uniqKey="Martinez Alonso D">D Martinez-Alonso</name>
</author>
<author><name sortKey="Dudognon, B" uniqKey="Dudognon B">B Dudognon</name>
</author>
<author><name sortKey="Guijarro, E" uniqKey="Guijarro E">E Guijarro</name>
</author>
<author><name sortKey="Escribano, Jm" uniqKey="Escribano J">JM Escribano</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Krammer, F" uniqKey="Krammer F">F Krammer</name>
</author>
<author><name sortKey="Schinko, T" uniqKey="Schinko T">T Schinko</name>
</author>
<author><name sortKey="Palmberger, D" uniqKey="Palmberger D">D Palmberger</name>
</author>
<author><name sortKey="Tauer, C" uniqKey="Tauer C">C Tauer</name>
</author>
<author><name sortKey="Messner, P" uniqKey="Messner P">P Messner</name>
</author>
<author><name sortKey="Grabherr, R" uniqKey="Grabherr R">R Grabherr</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Ignacio Casal, J" uniqKey="Ignacio Casal J">J Ignacio Casal</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Chung, Cy" uniqKey="Chung C">CY Chung</name>
</author>
<author><name sortKey="Chen, Cy" uniqKey="Chen C">CY Chen</name>
</author>
<author><name sortKey="Lin, Sy" uniqKey="Lin S">SY Lin</name>
</author>
<author><name sortKey="Chung, Yc" uniqKey="Chung Y">YC Chung</name>
</author>
<author><name sortKey="Chiu, Hy" uniqKey="Chiu H">HY Chiu</name>
</author>
<author><name sortKey="Chi, Wk" uniqKey="Chi W">WK Chi</name>
</author>
<author><name sortKey="Lin, Yl" uniqKey="Lin Y">YL Lin</name>
</author>
<author><name sortKey="Chiang, Bl" uniqKey="Chiang B">BL Chiang</name>
</author>
<author><name sortKey="Chen, Wj" uniqKey="Chen W">WJ Chen</name>
</author>
<author><name sortKey="Hu, Yc" uniqKey="Hu Y">YC Hu</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Bornstein, J" uniqKey="Bornstein J">J Bornstein</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Wang, Jw" uniqKey="Wang J">JW Wang</name>
</author>
<author><name sortKey="Roden, Rb" uniqKey="Roden R">RB Roden</name>
</author>
</analytic>
</biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct><analytic><author><name sortKey="Mohsen, Mo" uniqKey="Mohsen M">MO Mohsen</name>
</author>
<author><name sortKey="Zha, L" uniqKey="Zha L">L Zha</name>
</author>
<author><name sortKey="Cabral Miranda, G" uniqKey="Cabral Miranda G">G Cabral-Miranda</name>
</author>
<author><name sortKey="Bachmann, Mf" uniqKey="Bachmann M">MF Bachmann</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Liu, Yv" uniqKey="Liu Y">YV Liu</name>
</author>
<author><name sortKey="Massare, Mj" uniqKey="Massare M">MJ Massare</name>
</author>
<author><name sortKey="Pearce, Mb" uniqKey="Pearce M">MB Pearce</name>
</author>
<author><name sortKey="Sun, X" uniqKey="Sun X">X Sun</name>
</author>
<author><name sortKey="Belser, Ja" uniqKey="Belser J">JA Belser</name>
</author>
<author><name sortKey="Maines, Tr" uniqKey="Maines T">TR Maines</name>
</author>
<author><name sortKey="Creager, Hm" uniqKey="Creager H">HM Creager</name>
</author>
<author><name sortKey="Glenn, Gm" uniqKey="Glenn G">GM Glenn</name>
</author>
<author><name sortKey="Pushko, P" uniqKey="Pushko P">P Pushko</name>
</author>
<author><name sortKey="Smith, Ge" uniqKey="Smith G">GE Smith</name>
</author>
<author><name sortKey="Tumpey, Tm" uniqKey="Tumpey T">TM Tumpey</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kushnir, N" uniqKey="Kushnir N">N Kushnir</name>
</author>
<author><name sortKey="Streatfield, Sj" uniqKey="Streatfield S">SJ Streatfield</name>
</author>
<author><name sortKey="Yusibov, V" uniqKey="Yusibov V">V Yusibov</name>
</author>
</analytic>
</biblStruct>
<biblStruct></biblStruct>
<biblStruct><analytic><author><name sortKey="Pushko, P" uniqKey="Pushko P">P Pushko</name>
</author>
<author><name sortKey="Kort, T" uniqKey="Kort T">T Kort</name>
</author>
<author><name sortKey="Nathan, M" uniqKey="Nathan M">M Nathan</name>
</author>
<author><name sortKey="Pearce, Mb" uniqKey="Pearce M">MB Pearce</name>
</author>
<author><name sortKey="Smith, G" uniqKey="Smith G">G Smith</name>
</author>
<author><name sortKey="Tumpey, Tm" uniqKey="Tumpey T">TM Tumpey</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Bright, Ra" uniqKey="Bright R">RA Bright</name>
</author>
<author><name sortKey="Carter, Dm" uniqKey="Carter D">DM Carter</name>
</author>
<author><name sortKey="Daniluk, S" uniqKey="Daniluk S">S Daniluk</name>
</author>
<author><name sortKey="Toapanta, Fr" uniqKey="Toapanta F">FR Toapanta</name>
</author>
<author><name sortKey="Ahmad, A" uniqKey="Ahmad A">A Ahmad</name>
</author>
<author><name sortKey="Gavrilov, V" uniqKey="Gavrilov V">V Gavrilov</name>
</author>
<author><name sortKey="Massare, M" uniqKey="Massare M">M Massare</name>
</author>
<author><name sortKey="Pushko, P" uniqKey="Pushko P">P Pushko</name>
</author>
<author><name sortKey="Mytle, N" uniqKey="Mytle N">N Mytle</name>
</author>
<author><name sortKey="Rowe, T" uniqKey="Rowe T">T Rowe</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Cox, Mm" uniqKey="Cox M">MM Cox</name>
</author>
<author><name sortKey="Patriarca, Pa" uniqKey="Patriarca P">PA Patriarca</name>
</author>
<author><name sortKey="Treanor, J" uniqKey="Treanor J">J Treanor</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Cox, Mmir" uniqKey="Cox M">MMIR Cox</name>
</author>
<author><name sortKey="Post, P" uniqKey="Post P">P Post</name>
</author>
<author><name sortKey="Dunkle, L" uniqKey="Dunkle L">L Dunkle</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Chang, S Y" uniqKey="Chang S">S-Y Chang</name>
</author>
<author><name sortKey="Lin, P H" uniqKey="Lin P">P-H Lin</name>
</author>
<author><name sortKey="Tsai, J C" uniqKey="Tsai J">J-C Tsai</name>
</author>
<author><name sortKey="Hung, C C" uniqKey="Hung C">C-C Hung</name>
</author>
<author><name sortKey="Chang, S C" uniqKey="Chang S">S-C Chang</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Yang, Jr" uniqKey="Yang J">JR Yang</name>
</author>
<author><name sortKey="Kuo, Cy" uniqKey="Kuo C">CY Kuo</name>
</author>
<author><name sortKey="Huang, Hy" uniqKey="Huang H">HY Huang</name>
</author>
<author><name sortKey="Wu, Ft" uniqKey="Wu F">FT Wu</name>
</author>
<author><name sortKey="Huang, Yl" uniqKey="Huang Y">YL Huang</name>
</author>
<author><name sortKey="Cheng, Cy" uniqKey="Cheng C">CY Cheng</name>
</author>
<author><name sortKey="Su, Yt" uniqKey="Su Y">YT Su</name>
</author>
<author><name sortKey="Wu, Hs" uniqKey="Wu H">HS Wu</name>
</author>
<author><name sortKey="Liu, Mt" uniqKey="Liu M">MT Liu</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Wu, Cy" uniqKey="Wu C">CY Wu</name>
</author>
<author><name sortKey="Yeh, Yc" uniqKey="Yeh Y">YC Yeh</name>
</author>
<author><name sortKey="Yang, Yc" uniqKey="Yang Y">YC Yang</name>
</author>
<author><name sortKey="Chou, C" uniqKey="Chou C">C Chou</name>
</author>
<author><name sortKey="Liu, Mt" uniqKey="Liu M">MT Liu</name>
</author>
<author><name sortKey="Wu, Hs" uniqKey="Wu H">HS Wu</name>
</author>
<author><name sortKey="Chan, Jt" uniqKey="Chan J">JT Chan</name>
</author>
<author><name sortKey="Hsiao, Pw" uniqKey="Hsiao P">PW Hsiao</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Smith, Ge" uniqKey="Smith G">GE Smith</name>
</author>
<author><name sortKey="Flyer, Dc" uniqKey="Flyer D">DC Flyer</name>
</author>
<author><name sortKey="Raghunandan, R" uniqKey="Raghunandan R">R Raghunandan</name>
</author>
<author><name sortKey="Liu, Y" uniqKey="Liu Y">Y Liu</name>
</author>
<author><name sortKey="Wei, Z" uniqKey="Wei Z">Z Wei</name>
</author>
<author><name sortKey="Wu, Y" uniqKey="Wu Y">Y Wu</name>
</author>
<author><name sortKey="Kpamegan, E" uniqKey="Kpamegan E">E Kpamegan</name>
</author>
<author><name sortKey="Courbron, D" uniqKey="Courbron D">D Courbron</name>
</author>
<author><name sortKey="Fries, Lf" uniqKey="Fries L">LF Fries</name>
</author>
<author><name sortKey="Glenn, Gm" uniqKey="Glenn G">GM Glenn</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Chia, My" uniqKey="Chia M">MY Chia</name>
</author>
<author><name sortKey="Hu, Ay" uniqKey="Hu A">AY Hu</name>
</author>
<author><name sortKey="Tseng, Yf" uniqKey="Tseng Y">YF Tseng</name>
</author>
<author><name sortKey="Weng, Tc" uniqKey="Weng T">TC Weng</name>
</author>
<author><name sortKey="Lai, Cc" uniqKey="Lai C">CC Lai</name>
</author>
<author><name sortKey="Lin, Jy" uniqKey="Lin J">JY Lin</name>
</author>
<author><name sortKey="Chen, Pl" uniqKey="Chen P">PL Chen</name>
</author>
<author><name sortKey="Wang, Yf" uniqKey="Wang Y">YF Wang</name>
</author>
<author><name sortKey="Chao, Sr" uniqKey="Chao S">SR Chao</name>
</author>
<author><name sortKey="Chang, Jy" uniqKey="Chang J">JY Chang</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Fernandes Platzgummer, A" uniqKey="Fernandes Platzgummer A">A Fernandes-Platzgummer</name>
</author>
<author><name sortKey="Diogo, Mm" uniqKey="Diogo M">MM Diogo</name>
</author>
<author><name sortKey="Baptista, Rp" uniqKey="Baptista R">RP Baptista</name>
</author>
<author><name sortKey="Da Silva, Cl" uniqKey="Da Silva C">CL da Silva</name>
</author>
<author><name sortKey="Cabral, Jm" uniqKey="Cabral J">JM Cabral</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Hu, Ay" uniqKey="Hu A">AY Hu</name>
</author>
<author><name sortKey="Tseng, Yf" uniqKey="Tseng Y">YF Tseng</name>
</author>
<author><name sortKey="Weng, Tc" uniqKey="Weng T">TC Weng</name>
</author>
<author><name sortKey="Liao, Cc" uniqKey="Liao C">CC Liao</name>
</author>
<author><name sortKey="Wu, J" uniqKey="Wu J">J Wu</name>
</author>
<author><name sortKey="Chou, Ah" uniqKey="Chou A">AH Chou</name>
</author>
<author><name sortKey="Chao, Hj" uniqKey="Chao H">HJ Chao</name>
</author>
<author><name sortKey="Gu, A" uniqKey="Gu A">A Gu</name>
</author>
<author><name sortKey="Chen, J" uniqKey="Chen J">J Chen</name>
</author>
<author><name sortKey="Lin, Sc" uniqKey="Lin S">SC Lin</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Hidalgo, D" uniqKey="Hidalgo D">D Hidalgo</name>
</author>
<author><name sortKey="Paz, E" uniqKey="Paz E">E Paz</name>
</author>
<author><name sortKey="Palomares, La" uniqKey="Palomares L">LA Palomares</name>
</author>
<author><name sortKey="Ramirez, Ot" uniqKey="Ramirez O">OT Ramirez</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Zhang, Fsm" uniqKey="Zhang F">FSM Zhang</name>
</author>
<author><name sortKey="Itle, Lj" uniqKey="Itle L">LJ Itle</name>
</author>
<author><name sortKey="Lang, Sc" uniqKey="Lang S">SC Lang</name>
</author>
<author><name sortKey="Murhammer, Dw" uniqKey="Murhammer D">DW Murhammer</name>
</author>
<author><name sortKey="Linhardt, Rj" uniqKey="Linhardt R">RJ Linhardt</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Quan, Fs" uniqKey="Quan F">FS Quan</name>
</author>
<author><name sortKey="Lee, Yt" uniqKey="Lee Y">YT Lee</name>
</author>
<author><name sortKey="Kim, Kh" uniqKey="Kim K">KH Kim</name>
</author>
<author><name sortKey="Kim, Mc" uniqKey="Kim M">MC Kim</name>
</author>
<author><name sortKey="Kang, Sm" uniqKey="Kang S">SM Kang</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Li, C" uniqKey="Li C">C Li</name>
</author>
<author><name sortKey="Xu, K" uniqKey="Xu K">K Xu</name>
</author>
<author><name sortKey="Hashem, A" uniqKey="Hashem A">A Hashem</name>
</author>
<author><name sortKey="Shao, M" uniqKey="Shao M">M Shao</name>
</author>
<author><name sortKey="Liu, S" uniqKey="Liu S">S Liu</name>
</author>
<author><name sortKey="Zou, Y" uniqKey="Zou Y">Y Zou</name>
</author>
<author><name sortKey="Gao, Q" uniqKey="Gao Q">Q Gao</name>
</author>
<author><name sortKey="Zhang, Y" uniqKey="Zhang Y">Y Zhang</name>
</author>
<author><name sortKey="Yuan, L" uniqKey="Yuan L">L Yuan</name>
</author>
<author><name sortKey="Xu, M" uniqKey="Xu M">M Xu</name>
</author>
</analytic>
</biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct><analytic><author><name sortKey="Genzel, Y" uniqKey="Genzel Y">Y Genzel</name>
</author>
<author><name sortKey="Behrendt, I" uniqKey="Behrendt I">I Behrendt</name>
</author>
<author><name sortKey="Rodig, J" uniqKey="Rodig J">J Rodig</name>
</author>
<author><name sortKey="Rapp, E" uniqKey="Rapp E">E Rapp</name>
</author>
<author><name sortKey="Kueppers, C" uniqKey="Kueppers C">C Kueppers</name>
</author>
<author><name sortKey="Kochanek, S" uniqKey="Kochanek S">S Kochanek</name>
</author>
<author><name sortKey="Schiedner, G" uniqKey="Schiedner G">G Schiedner</name>
</author>
<author><name sortKey="Reichl, U" uniqKey="Reichl U">U Reichl</name>
</author>
</analytic>
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<pubmed><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Process development for pandemic influenza VLP vaccine production using a baculovirus expression system.</title>
<author><name sortKey="Lai, Chia Chun" sort="Lai, Chia Chun" uniqKey="Lai C" first="Chia-Chun" last="Lai">Chia-Chun Lai</name>
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<country xml:lang="fr">Taïwan</country>
<wicri:regionArea>National Institute of Infectious Diseases and Vaccinology, NHRI, 35 Keyan Road, Zhunan, Miaoli County</wicri:regionArea>
<wicri:noRegion>Miaoli County</wicri:noRegion>
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</author>
<author><name sortKey="Cheng, Yu Chieh" sort="Cheng, Yu Chieh" uniqKey="Cheng Y" first="Yu-Chieh" last="Cheng">Yu-Chieh Cheng</name>
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</author>
<author><name sortKey="Chen, Pin Wen" sort="Chen, Pin Wen" uniqKey="Chen P" first="Pin-Wen" last="Chen">Pin-Wen Chen</name>
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<country xml:lang="fr">Taïwan</country>
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</author>
<author><name sortKey="Lin, Ting Hui" sort="Lin, Ting Hui" uniqKey="Lin T" first="Ting-Hui" last="Lin">Ting-Hui Lin</name>
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<author><name sortKey="Tzeng, Tsai Teng" sort="Tzeng, Tsai Teng" uniqKey="Tzeng T" first="Tsai-Teng" last="Tzeng">Tsai-Teng Tzeng</name>
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<country xml:lang="fr">Taïwan</country>
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</author>
<author><name sortKey="Lu, Chia Chun" sort="Lu, Chia Chun" uniqKey="Lu C" first="Chia-Chun" last="Lu">Chia-Chun Lu</name>
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<wicri:noRegion>Miaoli County</wicri:noRegion>
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</author>
<author><name sortKey="Lee, Min Shi" sort="Lee, Min Shi" uniqKey="Lee M" first="Min-Shi" last="Lee">Min-Shi Lee</name>
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<author><name sortKey="Hu, Alan Yung Chih" sort="Hu, Alan Yung Chih" uniqKey="Hu A" first="Alan Yung-Chih" last="Hu">Alan Yung-Chih Hu</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en">Process development for pandemic influenza VLP vaccine production using a baculovirus expression system.</title>
<author><name sortKey="Lai, Chia Chun" sort="Lai, Chia Chun" uniqKey="Lai C" first="Chia-Chun" last="Lai">Chia-Chun Lai</name>
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<country xml:lang="fr">Taïwan</country>
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</author>
<author><name sortKey="Cheng, Yu Chieh" sort="Cheng, Yu Chieh" uniqKey="Cheng Y" first="Yu-Chieh" last="Cheng">Yu-Chieh Cheng</name>
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<country xml:lang="fr">Taïwan</country>
<wicri:regionArea>National Institute of Infectious Diseases and Vaccinology, NHRI, 35 Keyan Road, Zhunan, Miaoli County</wicri:regionArea>
<wicri:noRegion>Miaoli County</wicri:noRegion>
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</author>
<author><name sortKey="Chen, Pin Wen" sort="Chen, Pin Wen" uniqKey="Chen P" first="Pin-Wen" last="Chen">Pin-Wen Chen</name>
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<author><name sortKey="Lin, Ting Hui" sort="Lin, Ting Hui" uniqKey="Lin T" first="Ting-Hui" last="Lin">Ting-Hui Lin</name>
<affiliation wicri:level="1"><nlm:affiliation>National Institute of Infectious Diseases and Vaccinology, NHRI, 35 Keyan Road, Zhunan, Miaoli County, 35053 Taiwan.</nlm:affiliation>
<country xml:lang="fr">Taïwan</country>
<wicri:regionArea>National Institute of Infectious Diseases and Vaccinology, NHRI, 35 Keyan Road, Zhunan, Miaoli County</wicri:regionArea>
<wicri:noRegion>Miaoli County</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Tzeng, Tsai Teng" sort="Tzeng, Tsai Teng" uniqKey="Tzeng T" first="Tsai-Teng" last="Tzeng">Tsai-Teng Tzeng</name>
<affiliation wicri:level="1"><nlm:affiliation>National Institute of Infectious Diseases and Vaccinology, NHRI, 35 Keyan Road, Zhunan, Miaoli County, 35053 Taiwan.</nlm:affiliation>
<country xml:lang="fr">Taïwan</country>
<wicri:regionArea>National Institute of Infectious Diseases and Vaccinology, NHRI, 35 Keyan Road, Zhunan, Miaoli County</wicri:regionArea>
<wicri:noRegion>Miaoli County</wicri:noRegion>
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</author>
<author><name sortKey="Lu, Chia Chun" sort="Lu, Chia Chun" uniqKey="Lu C" first="Chia-Chun" last="Lu">Chia-Chun Lu</name>
<affiliation wicri:level="1"><nlm:affiliation>National Institute of Infectious Diseases and Vaccinology, NHRI, 35 Keyan Road, Zhunan, Miaoli County, 35053 Taiwan.</nlm:affiliation>
<country xml:lang="fr">Taïwan</country>
<wicri:regionArea>National Institute of Infectious Diseases and Vaccinology, NHRI, 35 Keyan Road, Zhunan, Miaoli County</wicri:regionArea>
<wicri:noRegion>Miaoli County</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Lee, Min Shi" sort="Lee, Min Shi" uniqKey="Lee M" first="Min-Shi" last="Lee">Min-Shi Lee</name>
<affiliation wicri:level="1"><nlm:affiliation>National Institute of Infectious Diseases and Vaccinology, NHRI, 35 Keyan Road, Zhunan, Miaoli County, 35053 Taiwan.</nlm:affiliation>
<country xml:lang="fr">Taïwan</country>
<wicri:regionArea>National Institute of Infectious Diseases and Vaccinology, NHRI, 35 Keyan Road, Zhunan, Miaoli County</wicri:regionArea>
<wicri:noRegion>Miaoli County</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Hu, Alan Yung Chih" sort="Hu, Alan Yung Chih" uniqKey="Hu A" first="Alan Yung-Chih" last="Hu">Alan Yung-Chih Hu</name>
<affiliation wicri:level="1"><nlm:affiliation>National Institute of Infectious Diseases and Vaccinology, NHRI, 35 Keyan Road, Zhunan, Miaoli County, 35053 Taiwan.</nlm:affiliation>
<country xml:lang="fr">Taïwan</country>
<wicri:regionArea>National Institute of Infectious Diseases and Vaccinology, NHRI, 35 Keyan Road, Zhunan, Miaoli County</wicri:regionArea>
<wicri:noRegion>Miaoli County</wicri:noRegion>
</affiliation>
</author>
</analytic>
<series><title level="j">Journal of biological engineering</title>
<idno type="ISSN">1754-1611</idno>
<imprint><date when="2019" type="published">2019</date>
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<front><div type="abstract" xml:lang="en">Influenza viruses cause hundreds of thousands of respiratory diseases worldwide each year, and vaccination is considered the most effective approach for preventing influenza annual epidemics or pandemics. Since 1950, chicken embryonated eggs have been used as the main method for producing seasonal influenza vaccines. However, this platform has the main drawback of a lack of scale-up flexibility, and thus, egg-based vaccine manufacturers cannot supply sufficient doses within a short period for use for pandemic prevention. As a result, strategies for reducing the manufacturing time and increasing production capacity are urgently needed. Non-virion vaccine methods have been considered an alternative strategy against an influenza pandemic, and the purpose of maintaining an immunogenic capsule structure with infectious properties appears to be met by the virus-like particle (VLP) platform.</div>
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