Process development for pandemic influenza VLP vaccine production using a baculovirus expression system.
Identifieur interne : 000103 ( PubMed/Checkpoint ); précédent : 000102; suivant : 000104Process development for pandemic influenza VLP vaccine production using a baculovirus expression system.
Auteurs : Chia-Chun Lai [Taïwan] ; Yu-Chieh Cheng [Taïwan] ; Pin-Wen Chen [Taïwan] ; Ting-Hui Lin [Taïwan] ; Tsai-Teng Tzeng [Taïwan] ; Chia-Chun Lu [Taïwan] ; Min-Shi Lee [Taïwan] ; Alan Yung-Chih Hu [Taïwan]Source :
- Journal of biological engineering [ 1754-1611 ] ; 2019.
Abstract
Influenza viruses cause hundreds of thousands of respiratory diseases worldwide each year, and vaccination is considered the most effective approach for preventing influenza annual epidemics or pandemics. Since 1950, chicken embryonated eggs have been used as the main method for producing seasonal influenza vaccines. However, this platform has the main drawback of a lack of scale-up flexibility, and thus, egg-based vaccine manufacturers cannot supply sufficient doses within a short period for use for pandemic prevention. As a result, strategies for reducing the manufacturing time and increasing production capacity are urgently needed. Non-virion vaccine methods have been considered an alternative strategy against an influenza pandemic, and the purpose of maintaining an immunogenic capsule structure with infectious properties appears to be met by the virus-like particle (VLP) platform.
DOI: 10.1186/s13036-019-0206-z
PubMed: 31666806
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County</wicri:regionArea><wicri:noRegion>Miaoli County</wicri:noRegion></affiliation></author><author><name sortKey="Cheng, Yu Chieh" sort="Cheng, Yu Chieh" uniqKey="Cheng Y" first="Yu-Chieh" last="Cheng">Yu-Chieh Cheng</name><affiliation wicri:level="1"><nlm:affiliation>National Institute of Infectious Diseases and Vaccinology, NHRI, 35 Keyan Road, Zhunan, Miaoli County, 35053 Taiwan.</nlm:affiliation><country xml:lang="fr">Taïwan</country><wicri:regionArea>National Institute of Infectious Diseases and Vaccinology, NHRI, 35 Keyan Road, Zhunan, Miaoli County</wicri:regionArea><wicri:noRegion>Miaoli County</wicri:noRegion></affiliation></author><author><name sortKey="Chen, Pin Wen" sort="Chen, Pin Wen" uniqKey="Chen P" first="Pin-Wen" last="Chen">Pin-Wen Chen</name><affiliation wicri:level="1"><nlm:affiliation>National Institute of Infectious Diseases and Vaccinology, NHRI, 35 Keyan Road, Zhunan, Miaoli County, 35053 Taiwan.</nlm:affiliation><country xml:lang="fr">Taïwan</country><wicri:regionArea>National Institute of Infectious Diseases and Vaccinology, NHRI, 35 Keyan Road, Zhunan, Miaoli County</wicri:regionArea><wicri:noRegion>Miaoli County</wicri:noRegion></affiliation></author><author><name sortKey="Lin, Ting Hui" sort="Lin, Ting Hui" uniqKey="Lin T" first="Ting-Hui" last="Lin">Ting-Hui Lin</name><affiliation wicri:level="1"><nlm:affiliation>National Institute of Infectious Diseases and Vaccinology, NHRI, 35 Keyan Road, Zhunan, Miaoli County, 35053 Taiwan.</nlm:affiliation><country xml:lang="fr">Taïwan</country><wicri:regionArea>National Institute of Infectious Diseases and Vaccinology, NHRI, 35 Keyan Road, Zhunan, Miaoli County</wicri:regionArea><wicri:noRegion>Miaoli County</wicri:noRegion></affiliation></author><author><name sortKey="Tzeng, Tsai Teng" sort="Tzeng, Tsai Teng" uniqKey="Tzeng T" first="Tsai-Teng" last="Tzeng">Tsai-Teng Tzeng</name><affiliation wicri:level="1"><nlm:affiliation>National Institute of Infectious Diseases and Vaccinology, NHRI, 35 Keyan Road, Zhunan, Miaoli County, 35053 Taiwan.</nlm:affiliation><country xml:lang="fr">Taïwan</country><wicri:regionArea>National Institute of Infectious Diseases and Vaccinology, NHRI, 35 Keyan Road, Zhunan, Miaoli County</wicri:regionArea><wicri:noRegion>Miaoli County</wicri:noRegion></affiliation></author><author><name sortKey="Lu, Chia Chun" sort="Lu, Chia Chun" uniqKey="Lu C" first="Chia-Chun" last="Lu">Chia-Chun Lu</name><affiliation wicri:level="1"><nlm:affiliation>National Institute of Infectious Diseases and Vaccinology, NHRI, 35 Keyan Road, Zhunan, Miaoli County, 35053 Taiwan.</nlm:affiliation><country xml:lang="fr">Taïwan</country><wicri:regionArea>National Institute of Infectious Diseases and Vaccinology, NHRI, 35 Keyan Road, Zhunan, Miaoli County</wicri:regionArea><wicri:noRegion>Miaoli County</wicri:noRegion></affiliation></author><author><name sortKey="Lee, Min Shi" sort="Lee, Min Shi" uniqKey="Lee M" first="Min-Shi" last="Lee">Min-Shi Lee</name><affiliation wicri:level="1"><nlm:affiliation>National Institute of Infectious Diseases and Vaccinology, NHRI, 35 Keyan Road, Zhunan, Miaoli County, 35053 Taiwan.</nlm:affiliation><country xml:lang="fr">Taïwan</country><wicri:regionArea>National Institute of Infectious Diseases and Vaccinology, NHRI, 35 Keyan Road, Zhunan, Miaoli County</wicri:regionArea><wicri:noRegion>Miaoli County</wicri:noRegion></affiliation></author><author><name sortKey="Hu, Alan Yung Chih" sort="Hu, Alan Yung Chih" uniqKey="Hu A" first="Alan Yung-Chih" last="Hu">Alan Yung-Chih Hu</name><affiliation wicri:level="1"><nlm:affiliation>National Institute of Infectious Diseases and Vaccinology, NHRI, 35 Keyan Road, Zhunan, Miaoli County, 35053 Taiwan.</nlm:affiliation><country xml:lang="fr">Taïwan</country><wicri:regionArea>National Institute of Infectious Diseases and Vaccinology, NHRI, 35 Keyan Road, Zhunan, Miaoli County</wicri:regionArea><wicri:noRegion>Miaoli County</wicri:noRegion></affiliation></author></analytic><series><title level="j">Journal of biological engineering</title><idno type="ISSN">1754-1611</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Influenza viruses cause hundreds of thousands of respiratory diseases worldwide each year, and vaccination is considered the most effective approach for preventing influenza annual epidemics or pandemics. Since 1950, chicken embryonated eggs have been used as the main method for producing seasonal influenza vaccines. However, this platform has the main drawback of a lack of scale-up flexibility, and thus, egg-based vaccine manufacturers cannot supply sufficient doses within a short period for use for pandemic prevention. As a result, strategies for reducing the manufacturing time and increasing production capacity are urgently needed. Non-virion vaccine methods have been considered an alternative strategy against an influenza pandemic, and the purpose of maintaining an immunogenic capsule structure with infectious properties appears to be met by the virus-like particle (VLP) platform.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">31666806</PMID><DateRevised><Year>2019</Year><Month>11</Month><Day>03</Day></DateRevised><Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Print">1754-1611</ISSN><JournalIssue CitedMedium="Print"><Volume>13</Volume><PubDate><Year>2019</Year></PubDate></JournalIssue><Title>Journal of biological engineering</Title><ISOAbbreviation>J Biol Eng</ISOAbbreviation></Journal><ArticleTitle>Process development for pandemic influenza VLP vaccine production using a baculovirus expression system.</ArticleTitle><Pagination><MedlinePgn>78</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1186/s13036-019-0206-z</ELocationID><Abstract><AbstractText Label="Background" NlmCategory="UNASSIGNED">Influenza viruses cause hundreds of thousands of respiratory diseases worldwide each year, and vaccination is considered the most effective approach for preventing influenza annual epidemics or pandemics. Since 1950, chicken embryonated eggs have been used as the main method for producing seasonal influenza vaccines. However, this platform has the main drawback of a lack of scale-up flexibility, and thus, egg-based vaccine manufacturers cannot supply sufficient doses within a short period for use for pandemic prevention. As a result, strategies for reducing the manufacturing time and increasing production capacity are urgently needed. Non-virion vaccine methods have been considered an alternative strategy against an influenza pandemic, and the purpose of maintaining an immunogenic capsule structure with infectious properties appears to be met by the virus-like particle (VLP) platform.</AbstractText><AbstractText Label="Results" NlmCategory="UNASSIGNED">An influenza H7N9-TW VLP production platform using insect cells, which included the expression of hemagglutinin (HA), NA, and M1 proteins, was established. To scale up H7N9-TW VLP production, several culture conditions were optimized to obtain a higher production yield. A high level of dissolved <i>oxygen (DO)</i> could be critical to H7N9-TW VLP production. If the DO was maintained at a high level, the HA titer obtained in the spinner flask system with ventilation was similar to that obtained in a shake flask. In this study, the HA titer in a 5-L bioreactor with a well-controlled DO level was substantially improved by 128-fold (from 4 HA units (HAU)/50 μL to 512 HAU/50 μL).</AbstractText><AbstractText Label="Conclusions" NlmCategory="UNASSIGNED">In this study, a multigene expression platform and an effective upstream process were developed. Notably, a high H7N9-TW VLP yield was achieved using a two-step production strategy while a high DO level was maintained. The upstream process, which resulted in high VLP titers, could be further used for large-scale influenza VLP vaccine production.</AbstractText><CopyrightInformation>© The Author(s). 2019.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Lai</LastName><ForeName>Chia-Chun</ForeName><Initials>CC</Initials><AffiliationInfo><Affiliation>National Institute of Infectious Diseases and Vaccinology, NHRI, 35 Keyan Road, Zhunan, Miaoli County, 35053 Taiwan.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>2College of Life Science, National Tsing Hua University, 101, Section 2, Kuang-Fu Road, Hsinchu, 30013 Taiwan.</Affiliation><Identifier Source="ISNI">0000 0004 0532 0580</Identifier><Identifier Source="GRID">grid.38348.34</Identifier></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Cheng</LastName><ForeName>Yu-Chieh</ForeName><Initials>YC</Initials><AffiliationInfo><Affiliation>National Institute of Infectious Diseases and Vaccinology, NHRI, 35 Keyan Road, Zhunan, Miaoli County, 35053 Taiwan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Pin-Wen</ForeName><Initials>PW</Initials><AffiliationInfo><Affiliation>National Institute of Infectious Diseases and Vaccinology, NHRI, 35 Keyan Road, Zhunan, Miaoli County, 35053 Taiwan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lin</LastName><ForeName>Ting-Hui</ForeName><Initials>TH</Initials><AffiliationInfo><Affiliation>National Institute of Infectious Diseases and Vaccinology, NHRI, 35 Keyan Road, Zhunan, Miaoli County, 35053 Taiwan.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>2College of Life Science, National Tsing Hua University, 101, Section 2, Kuang-Fu Road, Hsinchu, 30013 Taiwan.</Affiliation><Identifier Source="ISNI">0000 0004 0532 0580</Identifier><Identifier Source="GRID">grid.38348.34</Identifier></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Tzeng</LastName><ForeName>Tsai-Teng</ForeName><Initials>TT</Initials><AffiliationInfo><Affiliation>National Institute of Infectious Diseases and Vaccinology, NHRI, 35 Keyan Road, Zhunan, Miaoli County, 35053 Taiwan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lu</LastName><ForeName>Chia-Chun</ForeName><Initials>CC</Initials><AffiliationInfo><Affiliation>National Institute of Infectious Diseases and Vaccinology, NHRI, 35 Keyan Road, Zhunan, Miaoli County, 35053 Taiwan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lee</LastName><ForeName>Min-Shi</ForeName><Initials>MS</Initials><AffiliationInfo><Affiliation>National Institute of Infectious Diseases and Vaccinology, NHRI, 35 Keyan Road, Zhunan, Miaoli County, 35053 Taiwan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hu</LastName><ForeName>Alan Yung-Chih</ForeName><Initials>AY</Initials><AffiliationInfo><Affiliation>National Institute of Infectious Diseases and Vaccinology, NHRI, 35 Keyan Road, Zhunan, Miaoli County, 35053 Taiwan.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2019</Year><Month>10</Month><Day>23</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>J Biol Eng</MedlineTA><NlmUniqueID>101306640</NlmUniqueID><ISSNLinking>1754-1611</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Dissolved oxygen</Keyword><Keyword MajorTopicYN="N">Influenza vaccines</Keyword><Keyword MajorTopicYN="N">Virus-like particle</Keyword></KeywordList><CoiStatement>Competing interestsThe authors declare that they have no competing interests.</CoiStatement></MedlineCitation><PubmedData><History><PubMedPubDate 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first="Yu-Chieh" last="Cheng">Yu-Chieh Cheng</name><name sortKey="Hu, Alan Yung Chih" sort="Hu, Alan Yung Chih" uniqKey="Hu A" first="Alan Yung-Chih" last="Hu">Alan Yung-Chih Hu</name><name sortKey="Lee, Min Shi" sort="Lee, Min Shi" uniqKey="Lee M" first="Min-Shi" last="Lee">Min-Shi Lee</name><name sortKey="Lin, Ting Hui" sort="Lin, Ting Hui" uniqKey="Lin T" first="Ting-Hui" last="Lin">Ting-Hui Lin</name><name sortKey="Lu, Chia Chun" sort="Lu, Chia Chun" uniqKey="Lu C" first="Chia-Chun" last="Lu">Chia-Chun Lu</name><name sortKey="Tzeng, Tsai Teng" sort="Tzeng, Tsai Teng" uniqKey="Tzeng T" first="Tsai-Teng" last="Tzeng">Tsai-Teng Tzeng</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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