PandemieGrippaleV1, Ncbi, Merge, bibRecord, 001E17

Assessment of influenza virus exposure and recovery from contaminated surgical masks and N95 respirators

Identifieur interne : 001E17 ( Ncbi/Merge ); précédent : 001E16; suivant : 001E18

Assessment of influenza virus exposure and recovery from contaminated surgical masks and N95 respirators

Auteurs : Francoise M. Blachere [États-Unis] ; William G. Lindsley [États-Unis] ; Cynthia M. Mcmillen [États-Unis] ; Donald H. Beezhold [États-Unis] ; Edward M. Fisher [États-Unis] ; Ronald E. Shaffer [États-Unis] ; John D. Noti [États-Unis]

Source :

Journal of virological methods [ 0166-0934 ] ; 2018.

RBID : PMC:6482848

Descripteurs français

KwdFr :
- ARN viral (), ARN viral (génétique), Animaux, Aérosols, Cellules rénales canines Madin-Darby, Chiens, Dodécyl-sulfate de sodium (pharmacologie), Exposition professionnelle, Grippe humaine (), Grippe humaine (transmission), Humains, Masques (virologie), Personnel de santé, Respirateurs purificateurs d'air (virologie), Sérumalbumine bovine (pharmacologie), Taille de particule, Tensioactifs (pharmacologie), Virus de la grippe A (), Virus de la grippe A (génétique).
MESH :
- génétique : ARN viral, Virus de la grippe A.
- pharmacologie : Dodécyl-sulfate de sodium, Sérumalbumine bovine, Tensioactifs.
- virologie : Masques, Respirateurs purificateurs d'air.
- ARN viral, Animaux, Aérosols, Cellules rénales canines Madin-Darby, Chiens, Exposition professionnelle, Grippe humaine, Humains, Personnel de santé, Taille de particule, Virus de la grippe A.

English descriptors

KwdEn :
- Aerosols, Animals, Dogs, Health Personnel, Humans, Influenza A virus (drug effects), Influenza A virus (genetics), Influenza, Human (prevention & control), Influenza, Human (transmission), Madin Darby Canine Kidney Cells, Masks (virology), Occupational Exposure, Particle Size, RNA, Viral (drug effects), RNA, Viral (genetics), Respiratory Protective Devices (virology), Serum Albumin, Bovine (pharmacology), Sodium Dodecyl Sulfate (pharmacology), Surface-Active Agents (pharmacology).
MESH :
- chemical , drug effects : RNA, Viral.
- chemical , genetics : RNA, Viral.
- chemical , pharmacology : Serum Albumin, Bovine, Sodium Dodecyl Sulfate, Surface-Active Agents.
- chemical : Aerosols.
- drug effects : Influenza A virus.
- genetics : Influenza A virus.
- prevention & control : Influenza, Human.
- transmission : Influenza, Human.
- virology : Masks, Respiratory Protective Devices.
- Animals, Dogs, Health Personnel, Humans, Madin Darby Canine Kidney Cells, Occupational Exposure, Particle Size.

Abstract

Healthcare workers (HCWs) are at significantly higher risk of exposure to influenza virus during seasonal epidemics and global pandemics. During the 2009 influenza pandemic, some healthcare organizations recommended that HCWs wear respiratory protection such as filtering facepiece respirators, while others indicated that facemasks such as surgical masks (SMs) were sufficient. To assess the level of exposure a HCW may possibly encounter, the aim of this study was to (1.) evaluate if SMs and N95 respirators can serve as “personal bioaerosol samplers” for influenza virus and (2.) determine if SMs and N95 respirators contaminated by influenza laden aerosols can serve as a source of infectious virus for indirect contact transmission. This effort is part of a National Institute for Occupational Safety and Health 5-year multidisciplinary study to determine the routes of influenza transmission in healthcare settings. A coughing simulator was programmed to cough aerosol particles containing influenza virus over a wide concentration range into an aerosol exposure simulation chamber virus/L of exam room air), and a breathing simulator was used to collect virus on either a SM or N95 respirator. Extraction buffers containing nonionic and anionic detergents as well as various protein additives were used to recover influenza virus from the masks and respirators. The inclusion of 0.1% SDS resulted in maximal influenza RNA recovery (41.3%) but with a complete loss of infectivity whereas inclusion of 0.1% bovine serum albumin resulted in reduced RNA recovery (6.8%) but maximal retention of virus infectivity (17.9%). Our results show that a HCW’s potential exposure to airborne influenza virus can be assessed in part through analysis of their SMs and N95 respirators, which can effectively serve as personal bioaerosol samplers.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6482848

DOI: 10.1016/j.jviromet.2018.05.009
PubMed: 30029810
PubMed Central: 6482848

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PMC:6482848

Le document en format XML

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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Aerosols</term>
<term>Animals</term>
<term>Dogs</term>
<term>Health Personnel</term>
<term>Humans</term>
<term>Influenza A virus (drug effects)</term>
<term>Influenza A virus (genetics)</term>
<term>Influenza, Human (prevention & control)</term>
<term>Influenza, Human (transmission)</term>
<term>Madin Darby Canine Kidney Cells</term>
<term>Masks (virology)</term>
<term>Occupational Exposure</term>
<term>Particle Size</term>
<term>RNA, Viral (drug effects)</term>
<term>RNA, Viral (genetics)</term>
<term>Respiratory Protective Devices (virology)</term>
<term>Serum Albumin, Bovine (pharmacology)</term>
<term>Sodium Dodecyl Sulfate (pharmacology)</term>
<term>Surface-Active Agents (pharmacology)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>ARN viral ()</term>
<term>ARN viral (génétique)</term>
<term>Animaux</term>
<term>Aérosols</term>
<term>Cellules rénales canines Madin-Darby</term>
<term>Chiens</term>
<term>Dodécyl-sulfate de sodium (pharmacologie)</term>
<term>Exposition professionnelle</term>
<term>Grippe humaine ()</term>
<term>Grippe humaine (transmission)</term>
<term>Humains</term>
<term>Masques (virologie)</term>
<term>Personnel de santé</term>
<term>Respirateurs purificateurs d'air (virologie)</term>
<term>Sérumalbumine bovine (pharmacologie)</term>
<term>Taille de particule</term>
<term>Tensioactifs (pharmacologie)</term>
<term>Virus de la grippe A ()</term>
<term>Virus de la grippe A (génétique)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="drug effects" xml:lang="en"><term>RNA, Viral</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>RNA, Viral</term>
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<keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Serum Albumin, Bovine</term>
<term>Sodium Dodecyl Sulfate</term>
<term>Surface-Active Agents</term>
</keywords>
<keywords scheme="MESH" type="chemical" xml:lang="en"><term>Aerosols</term>
</keywords>
<keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Influenza A virus</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Influenza A virus</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ARN viral</term>
<term>Virus de la grippe A</term>
</keywords>
<keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Dodécyl-sulfate de sodium</term>
<term>Sérumalbumine bovine</term>
<term>Tensioactifs</term>
</keywords>
<keywords scheme="MESH" qualifier="prevention & control" xml:lang="en"><term>Influenza, Human</term>
</keywords>
<keywords scheme="MESH" qualifier="transmission" xml:lang="en"><term>Influenza, Human</term>
</keywords>
<keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Masques</term>
<term>Respirateurs purificateurs d'air</term>
</keywords>
<keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Masks</term>
<term>Respiratory Protective Devices</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Dogs</term>
<term>Health Personnel</term>
<term>Humans</term>
<term>Madin Darby Canine Kidney Cells</term>
<term>Occupational Exposure</term>
<term>Particle Size</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>ARN viral</term>
<term>Animaux</term>
<term>Aérosols</term>
<term>Cellules rénales canines Madin-Darby</term>
<term>Chiens</term>
<term>Exposition professionnelle</term>
<term>Grippe humaine</term>
<term>Humains</term>
<term>Personnel de santé</term>
<term>Taille de particule</term>
<term>Virus de la grippe A</term>
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<front><div type="abstract" xml:lang="en"><p id="P1">Healthcare workers (HCWs) are at significantly higher risk of exposure to influenza virus during seasonal epidemics and global pandemics. During the 2009 influenza pandemic, some healthcare organizations recommended that HCWs wear respiratory protection such as filtering facepiece respirators, while others indicated that facemasks such as surgical masks (SMs) were sufficient. To assess the level of exposure a HCW may possibly encounter, the aim of this study was to (1.) evaluate if SMs and N95 respirators can serve as “personal bioaerosol samplers” for influenza virus and (2.) determine if SMs and N95 respirators contaminated by influenza laden aerosols can serve as a source of infectious virus for indirect contact transmission. This effort is part of a National Institute for Occupational Safety and Health 5-year multidisciplinary study to determine the routes of influenza transmission in healthcare settings. A coughing simulator was programmed to cough aerosol particles containing influenza virus over a wide concentration range into an aerosol exposure simulation chamber virus/L of exam room air), and a breathing simulator was used to collect virus on either a SM or N95 respirator. Extraction buffers containing nonionic and anionic detergents as well as various protein additives were used to recover influenza virus from the masks and respirators. The inclusion of 0.1% SDS resulted in maximal influenza RNA recovery (41.3%) but with a complete loss of infectivity whereas inclusion of 0.1% bovine serum albumin resulted in reduced RNA recovery (6.8%) but maximal retention of virus infectivity (17.9%). Our results show that a HCW’s potential exposure to airborne influenza virus can be assessed in part through analysis of their SMs and N95 respirators, which can effectively serve as personal bioaerosol samplers.</p>
</div>
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<affiliation wicri:level="2"><nlm:aff id="A2"> Center for Vaccine Research, Infectious Diseases and Microbiology, University of Pittsburgh Graduate School of Public Health, University of Pittsburgh, Pittsburgh, PA, USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
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<country xml:lang="fr">États-Unis</country>
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<country xml:lang="fr">États-Unis</country>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Assessment of influenza virus exposure and recovery from contaminated surgical masks and N95 respirators</title>
<author><name sortKey="Blachere, Francoise M" sort="Blachere, Francoise M" uniqKey="Blachere F" first="Francoise M." last="Blachere">Francoise M. Blachere</name>
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<country xml:lang="fr">États-Unis</country>
<wicri:regionArea> Allergy and Clinical Immunology Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Centers for Disease Control and Prevention, Morgantown, WV</wicri:regionArea>
<placeName><region type="state">Virginie-Occidentale</region>
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</author>
<author><name sortKey="Lindsley, William G" sort="Lindsley, William G" uniqKey="Lindsley W" first="William G." last="Lindsley">William G. Lindsley</name>
<affiliation wicri:level="2"><nlm:aff id="A1"> Allergy and Clinical Immunology Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Centers for Disease Control and Prevention, Morgantown, WV, USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea> Allergy and Clinical Immunology Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Centers for Disease Control and Prevention, Morgantown, WV</wicri:regionArea>
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<author><name sortKey="Mcmillen, Cynthia M" sort="Mcmillen, Cynthia M" uniqKey="Mcmillen C" first="Cynthia M." last="Mcmillen">Cynthia M. Mcmillen</name>
<affiliation wicri:level="2"><nlm:aff id="A1"> Allergy and Clinical Immunology Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Centers for Disease Control and Prevention, Morgantown, WV, USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea> Allergy and Clinical Immunology Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Centers for Disease Control and Prevention, Morgantown, WV</wicri:regionArea>
<placeName><region type="state">Virginie-Occidentale</region>
</placeName>
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<affiliation wicri:level="2"><nlm:aff id="A2"> Center for Vaccine Research, Infectious Diseases and Microbiology, University of Pittsburgh Graduate School of Public Health, University of Pittsburgh, Pittsburgh, PA, USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea> Center for Vaccine Research, Infectious Diseases and Microbiology, University of Pittsburgh Graduate School of Public Health, University of Pittsburgh, Pittsburgh, PA</wicri:regionArea>
<placeName><region type="state">Pennsylvanie</region>
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</affiliation>
</author>
<author><name sortKey="Beezhold, Donald H" sort="Beezhold, Donald H" uniqKey="Beezhold D" first="Donald H." last="Beezhold">Donald H. Beezhold</name>
<affiliation wicri:level="2"><nlm:aff id="A1"> Allergy and Clinical Immunology Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Centers for Disease Control and Prevention, Morgantown, WV, USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea> Allergy and Clinical Immunology Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Centers for Disease Control and Prevention, Morgantown, WV</wicri:regionArea>
<placeName><region type="state">Virginie-Occidentale</region>
</placeName>
</affiliation>
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<author><name sortKey="Fisher, Edward M" sort="Fisher, Edward M" uniqKey="Fisher E" first="Edward M." last="Fisher">Edward M. Fisher</name>
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<country xml:lang="fr">États-Unis</country>
<wicri:regionArea> National Personal Protective Technology Laboratory, National Institute for Occupational Safety and Health, Pittsburgh, PA</wicri:regionArea>
<placeName><region type="state">Pennsylvanie</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Shaffer, Ronald E" sort="Shaffer, Ronald E" uniqKey="Shaffer R" first="Ronald E." last="Shaffer">Ronald E. Shaffer</name>
<affiliation wicri:level="2"><nlm:aff id="A3"> National Personal Protective Technology Laboratory, National Institute for Occupational Safety and Health, Pittsburgh, PA, USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea> National Personal Protective Technology Laboratory, National Institute for Occupational Safety and Health, Pittsburgh, PA</wicri:regionArea>
<placeName><region type="state">Pennsylvanie</region>
</placeName>
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</author>
<author><name sortKey="Noti, John D" sort="Noti, John D" uniqKey="Noti J" first="John D." last="Noti">John D. Noti</name>
<affiliation wicri:level="2"><nlm:aff id="A1"> Allergy and Clinical Immunology Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Centers for Disease Control and Prevention, Morgantown, WV, USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea> Allergy and Clinical Immunology Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Centers for Disease Control and Prevention, Morgantown, WV</wicri:regionArea>
<placeName><region type="state">Virginie-Occidentale</region>
</placeName>
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<series><title level="j">Journal of virological methods</title>
<idno type="ISSN">0166-0934</idno>
<idno type="eISSN">1879-0984</idno>
<imprint><date when="2018">2018</date>
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<front><div type="abstract" xml:lang="en"><p id="P1">Healthcare workers (HCWs) are at significantly higher risk of exposure to influenza virus during seasonal epidemics and global pandemics. During the 2009 influenza pandemic, some healthcare organizations recommended that HCWs wear respiratory protection such as filtering facepiece respirators, while others indicated that facemasks such as surgical masks (SMs) were sufficient. To assess the level of exposure a HCW may possibly encounter, the aim of this study was to (1.) evaluate if SMs and N95 respirators can serve as “personal bioaerosol samplers” for influenza virus and (2.) determine if SMs and N95 respirators contaminated by influenza laden aerosols can serve as a source of infectious virus for indirect contact transmission. This effort is part of a National Institute for Occupational Safety and Health 5-year multidisciplinary study to determine the routes of influenza transmission in healthcare settings. A coughing simulator was programmed to cough aerosol particles containing influenza virus over a wide concentration range into an aerosol exposure simulation chamber virus/L of exam room air), and a breathing simulator was used to collect virus on either a SM or N95 respirator. Extraction buffers containing nonionic and anionic detergents as well as various protein additives were used to recover influenza virus from the masks and respirators. The inclusion of 0.1% SDS resulted in maximal influenza RNA recovery (41.3%) but with a complete loss of infectivity whereas inclusion of 0.1% bovine serum albumin resulted in reduced RNA recovery (6.8%) but maximal retention of virus infectivity (17.9%). Our results show that a HCW’s potential exposure to airborne influenza virus can be assessed in part through analysis of their SMs and N95 respirators, which can effectively serve as personal bioaerosol samplers.</p>
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<affiliation wicri:level="2"><nlm:affiliation>Allergy and Clinical Immunology Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Centers for Disease Control and Prevention, Morgantown, WV, USA. Electronic address: fblachere@cdc.gov.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
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<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Allergy and Clinical Immunology Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Centers for Disease Control and Prevention, Morgantown, WV</wicri:regionArea>
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<author><name sortKey="Mcmillen, Cynthia M" sort="Mcmillen, Cynthia M" uniqKey="Mcmillen C" first="Cynthia M" last="Mcmillen">Cynthia M. Mcmillen</name>
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<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Allergy and Clinical Immunology Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Centers for Disease Control and Prevention, Morgantown, WV, USA; Center for Vaccine Research, Infectious Diseases and Microbiology, University of Pittsburgh Graduate School of Public Health, University of Pittsburgh, Pittsburgh, PA</wicri:regionArea>
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</placeName>
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<author><name sortKey="Beezhold, Donald H" sort="Beezhold, Donald H" uniqKey="Beezhold D" first="Donald H" last="Beezhold">Donald H. Beezhold</name>
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<country xml:lang="fr">États-Unis</country>
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<author><name sortKey="Fisher, Edward M" sort="Fisher, Edward M" uniqKey="Fisher E" first="Edward M" last="Fisher">Edward M. Fisher</name>
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<author><name sortKey="Shaffer, Ronald E" sort="Shaffer, Ronald E" uniqKey="Shaffer R" first="Ronald E" last="Shaffer">Ronald E. Shaffer</name>
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<author><name sortKey="Noti, John D" sort="Noti, John D" uniqKey="Noti J" first="John D" last="Noti">John D. Noti</name>
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<country xml:lang="fr">États-Unis</country>
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<author><name sortKey="Mcmillen, Cynthia M" sort="Mcmillen, Cynthia M" uniqKey="Mcmillen C" first="Cynthia M" last="Mcmillen">Cynthia M. Mcmillen</name>
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<author><name sortKey="Noti, John D" sort="Noti, John D" uniqKey="Noti J" first="John D" last="Noti">John D. Noti</name>
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<term>Animals</term>
<term>Dogs</term>
<term>Health Personnel</term>
<term>Humans</term>
<term>Influenza A virus (drug effects)</term>
<term>Influenza A virus (genetics)</term>
<term>Influenza, Human (prevention & control)</term>
<term>Influenza, Human (transmission)</term>
<term>Madin Darby Canine Kidney Cells</term>
<term>Masks (virology)</term>
<term>Occupational Exposure</term>
<term>Particle Size</term>
<term>RNA, Viral (drug effects)</term>
<term>RNA, Viral (genetics)</term>
<term>Respiratory Protective Devices (virology)</term>
<term>Serum Albumin, Bovine (pharmacology)</term>
<term>Sodium Dodecyl Sulfate (pharmacology)</term>
<term>Surface-Active Agents (pharmacology)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>ARN viral ()</term>
<term>ARN viral (génétique)</term>
<term>Animaux</term>
<term>Aérosols</term>
<term>Cellules rénales canines Madin-Darby</term>
<term>Chiens</term>
<term>Dodécyl-sulfate de sodium (pharmacologie)</term>
<term>Exposition professionnelle</term>
<term>Grippe humaine ()</term>
<term>Grippe humaine (transmission)</term>
<term>Humains</term>
<term>Masques (virologie)</term>
<term>Personnel de santé</term>
<term>Respirateurs purificateurs d'air (virologie)</term>
<term>Sérumalbumine bovine (pharmacologie)</term>
<term>Taille de particule</term>
<term>Tensioactifs (pharmacologie)</term>
<term>Virus de la grippe A ()</term>
<term>Virus de la grippe A (génétique)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="drug effects" xml:lang="en"><term>RNA, Viral</term>
</keywords>
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</keywords>
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<term>Surface-Active Agents</term>
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</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Influenza A virus</term>
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<term>Virus de la grippe A</term>
</keywords>
<keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Dodécyl-sulfate de sodium</term>
<term>Sérumalbumine bovine</term>
<term>Tensioactifs</term>
</keywords>
<keywords scheme="MESH" qualifier="prevention & control" xml:lang="en"><term>Influenza, Human</term>
</keywords>
<keywords scheme="MESH" qualifier="transmission" xml:lang="en"><term>Influenza, Human</term>
</keywords>
<keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Masques</term>
<term>Respirateurs purificateurs d'air</term>
</keywords>
<keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Masks</term>
<term>Respiratory Protective Devices</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Dogs</term>
<term>Health Personnel</term>
<term>Humans</term>
<term>Madin Darby Canine Kidney Cells</term>
<term>Occupational Exposure</term>
<term>Particle Size</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>ARN viral</term>
<term>Animaux</term>
<term>Aérosols</term>
<term>Cellules rénales canines Madin-Darby</term>
<term>Chiens</term>
<term>Exposition professionnelle</term>
<term>Grippe humaine</term>
<term>Humains</term>
<term>Personnel de santé</term>
<term>Taille de particule</term>
<term>Virus de la grippe A</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Healthcare workers (HCWs) are at significantly higher risk of exposure to influenza virus during seasonal epidemics and global pandemics. During the 2009 influenza pandemic, some healthcare organizations recommended that HCWs wear respiratory protection such as filtering facepiece respirators, while others indicated that facemasks such as surgical masks (SMs) were sufficient. To assess the level of exposure a HCW may possibly encounter, the aim of this study was to (1.) evaluate if SMs and N95 respirators can serve as "personal bioaerosol samplers" for influenza virus and (2.) determine if SMs and N95 respirators contaminated by influenza laden aerosols can serve as a source of infectious virus for indirect contact transmission. This effort is part of a National Institute for Occupational Safety and Health 5-year multidisciplinary study to determine the routes of influenza transmission in healthcare settings. A coughing simulator was programmed to cough aerosol particles containing influenza virus over a wide concentration range into an aerosol exposure simulation chamber virus/L of exam room air), and a breathing simulator was used to collect virus on either a SM or N95 respirator. Extraction buffers containing nonionic and anionic detergents as well as various protein additives were used to recover influenza virus from the masks and respirators. The inclusion of 0.1% SDS resulted in maximal influenza RNA recovery (41.3%) but with a complete loss of infectivity whereas inclusion of 0.1% bovine serum albumin resulted in reduced RNA recovery (6.8%) but maximal retention of virus infectivity (17.9%). Our results show that a HCW's potential exposure to airborne influenza virus can be assessed in part through analysis of their SMs and N95 respirators, which can effectively serve as personal bioaerosol samplers.</div>
</front>
</TEI>
</pubmed>
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	Serveur d'exploration sur les pandémies grippales
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Assessment of influenza virus exposure and recovery from contaminated surgical masks and N95 respirators

Assessment of influenza virus exposure and recovery from contaminated surgical masks and N95 respirators

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