A clinical utility of a strip test for influenza A/B and comparison with detection by RT PCR.
Identifieur interne : 001924 ( Ncbi/Merge ); précédent : 001923; suivant : 001925A clinical utility of a strip test for influenza A/B and comparison with detection by RT PCR.
Auteurs : Maciej Miarka [Pologne] ; Andrzej Horban [Pologne] ; Henryka Maliszewska [Pologne] ; Przemysław Bili Ski [Pologne] ; Wanda Prus-Kowalczuk [Pologne]Source :
- Acta biochimica Polonica [ 1734-154X ] ; 2014.
Descripteurs français
- KwdFr :
- Adolescent, Adulte, Adulte d'âge moyen, Bandelettes réactives, Enfant, Femelle, Grippe humaine (diagnostic), Grippe humaine (virologie), Humains, Jeune adulte, Mâle, RT-PCR (), Sensibilité et spécificité, Sous-type H1N1 du virus de la grippe A (isolement et purification), Sujet âgé, Sujet âgé de 80 ans ou plus, Virus influenza B (isolement et purification).
- MESH :
- diagnostic : Grippe humaine.
- isolement et purification : Sous-type H1N1 du virus de la grippe A, Virus influenza B.
- virologie : Grippe humaine.
- Adolescent, Adulte, Adulte d'âge moyen, Bandelettes réactives, Enfant, Femelle, Humains, Jeune adulte, Mâle, RT-PCR, Sensibilité et spécificité, Sujet âgé, Sujet âgé de 80 ans ou plus.
English descriptors
- KwdEn :
- Adolescent, Adult, Aged, Aged, 80 and over, Child, Female, Humans, Influenza A Virus, H1N1 Subtype (isolation & purification), Influenza B virus (isolation & purification), Influenza, Human (diagnosis), Influenza, Human (virology), Male, Middle Aged, Reagent Strips, Reverse Transcriptase Polymerase Chain Reaction (methods), Sensitivity and Specificity, Young Adult.
- MESH :
- chemical : Reagent Strips.
- diagnosis : Influenza, Human.
- isolation & purification : Influenza A Virus, H1N1 Subtype, Influenza B virus.
- methods : Reverse Transcriptase Polymerase Chain Reaction.
- virology : Influenza, Human.
- Adolescent, Adult, Aged, Aged, 80 and over, Child, Female, Humans, Male, Middle Aged, Sensitivity and Specificity, Young Adult.
Abstract
In June 2009 the World Health Organization announced influenza pandemic caused by A/H1N1/v virus. It became crucial to recognize new cases of A/H1N1/v infection. An effective screening diagnostic procedure was needed for patients suffering from influenza-like symptoms for making an initial diagnosis and analyzing epidemiological pattern of infection. We used a strip test for influenza A/B as a screening diagnostic procedure for patients suffering from influenza-like symptoms for making an initial diagnosis. For comparison, RT PCR for detecting A/H1N1/v was performed. The aim of this study was to assess the efficacy and sensitivity of the strip test and its value for making initial diagnosis of influenza A/H1N1/v.
PubMed: 25210936
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pubmed:25210936Le document en format XML
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<term>Aged, 80 and over</term>
<term>Child</term>
<term>Female</term>
<term>Humans</term>
<term>Influenza A Virus, H1N1 Subtype (isolation & purification)</term>
<term>Influenza B virus (isolation & purification)</term>
<term>Influenza, Human (diagnosis)</term>
<term>Influenza, Human (virology)</term>
<term>Male</term>
<term>Middle Aged</term>
<term>Reagent Strips</term>
<term>Reverse Transcriptase Polymerase Chain Reaction (methods)</term>
<term>Sensitivity and Specificity</term>
<term>Young Adult</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Adolescent</term>
<term>Adulte</term>
<term>Adulte d'âge moyen</term>
<term>Bandelettes réactives</term>
<term>Enfant</term>
<term>Femelle</term>
<term>Grippe humaine (diagnostic)</term>
<term>Grippe humaine (virologie)</term>
<term>Humains</term>
<term>Jeune adulte</term>
<term>Mâle</term>
<term>RT-PCR ()</term>
<term>Sensibilité et spécificité</term>
<term>Sous-type H1N1 du virus de la grippe A (isolement et purification)</term>
<term>Sujet âgé</term>
<term>Sujet âgé de 80 ans ou plus</term>
<term>Virus influenza B (isolement et purification)</term>
</keywords>
<keywords scheme="MESH" type="chemical" xml:lang="en"><term>Reagent Strips</term>
</keywords>
<keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Influenza, Human</term>
</keywords>
<keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Grippe humaine</term>
</keywords>
<keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Influenza A Virus, H1N1 Subtype</term>
<term>Influenza B virus</term>
</keywords>
<keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Sous-type H1N1 du virus de la grippe A</term>
<term>Virus influenza B</term>
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<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Reverse Transcriptase Polymerase Chain Reaction</term>
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<keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Grippe humaine</term>
</keywords>
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</keywords>
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<term>Adult</term>
<term>Aged</term>
<term>Aged, 80 and over</term>
<term>Child</term>
<term>Female</term>
<term>Humans</term>
<term>Male</term>
<term>Middle Aged</term>
<term>Sensitivity and Specificity</term>
<term>Young Adult</term>
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<term>Adulte</term>
<term>Adulte d'âge moyen</term>
<term>Bandelettes réactives</term>
<term>Enfant</term>
<term>Femelle</term>
<term>Humains</term>
<term>Jeune adulte</term>
<term>Mâle</term>
<term>RT-PCR</term>
<term>Sensibilité et spécificité</term>
<term>Sujet âgé</term>
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<front><div type="abstract" xml:lang="en">In June 2009 the World Health Organization announced influenza pandemic caused by A/H1N1/v virus. It became crucial to recognize new cases of A/H1N1/v infection. An effective screening diagnostic procedure was needed for patients suffering from influenza-like symptoms for making an initial diagnosis and analyzing epidemiological pattern of infection. We used a strip test for influenza A/B as a screening diagnostic procedure for patients suffering from influenza-like symptoms for making an initial diagnosis. For comparison, RT PCR for detecting A/H1N1/v was performed. The aim of this study was to assess the efficacy and sensitivity of the strip test and its value for making initial diagnosis of influenza A/H1N1/v.</div>
</front>
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<Title>Acta biochimica Polonica</Title>
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<ArticleTitle>A clinical utility of a strip test for influenza A/B and comparison with detection by RT PCR.</ArticleTitle>
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<Abstract><AbstractText Label="INTRODUCTION AND OBJECTIVE" NlmCategory="OBJECTIVE">In June 2009 the World Health Organization announced influenza pandemic caused by A/H1N1/v virus. It became crucial to recognize new cases of A/H1N1/v infection. An effective screening diagnostic procedure was needed for patients suffering from influenza-like symptoms for making an initial diagnosis and analyzing epidemiological pattern of infection. We used a strip test for influenza A/B as a screening diagnostic procedure for patients suffering from influenza-like symptoms for making an initial diagnosis. For comparison, RT PCR for detecting A/H1N1/v was performed. The aim of this study was to assess the efficacy and sensitivity of the strip test and its value for making initial diagnosis of influenza A/H1N1/v.</AbstractText>
<AbstractText Label="MATERIAL AND METHODS" NlmCategory="METHODS">Strip testing for the influenza A/B infection was performed on 1123 patients with influenza-like symptoms in the Admission Unit of the Regional Infectious Diseases Hospital in Warsaw. Strip test results were analyzed according to the age of patients and season of the year. For 97 patients strip test results for detecting A/H1N1 infection were compared with those obtained by RT PCR.</AbstractText>
<AbstractText Label="RESULTS" NlmCategory="RESULTS">There were no statistically significant differences found between the methods and strip testing demonstrated sensitivity of 61% and specificity of 71%.</AbstractText>
<AbstractText Label="CONCLUSIONS" NlmCategory="CONCLUSIONS">No statistically significant differences were found between the two methods, however, strip test had low sensitivity and specificity.</AbstractText>
</Abstract>
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<ForeName>Maciej</ForeName>
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<AffiliationInfo><Affiliation>Regional Infectious Diseases Hospital, Warsaw, Poland.</Affiliation>
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<Author ValidYN="Y"><LastName>Horban</LastName>
<ForeName>Andrzej</ForeName>
<Initials>A</Initials>
<AffiliationInfo><Affiliation>Regional Infectious Diseases Hospital, Warsaw, Poland.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Maliszewska</LastName>
<ForeName>Henryka</ForeName>
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<AffiliationInfo><Affiliation>Regional Infectious Diseases Hospital, Warsaw, Poland.</Affiliation>
</AffiliationInfo>
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<Author ValidYN="Y"><LastName>Biliński</LastName>
<ForeName>Przemysław</ForeName>
<Initials>P</Initials>
<AffiliationInfo><Affiliation>Chief Sanitary Inspectorate, Warsaw, Poland.</Affiliation>
</AffiliationInfo>
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<Author ValidYN="Y"><LastName>Prus-Kowalczuk</LastName>
<ForeName>Wanda</ForeName>
<Initials>W</Initials>
<AffiliationInfo><Affiliation>Regional Infectious Diseases Hospital, Warsaw, Poland.</Affiliation>
</AffiliationInfo>
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<MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName>
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<MeshHeading><DescriptorName UI="D053118" MajorTopicYN="N">Influenza A Virus, H1N1 Subtype</DescriptorName>
<QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D009981" MajorTopicYN="N">Influenza B virus</DescriptorName>
<QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D007251" MajorTopicYN="N">Influenza, Human</DescriptorName>
<QualifierName UI="Q000175" MajorTopicYN="Y">diagnosis</QualifierName>
<QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName>
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<MeshHeading><DescriptorName UI="D008297" MajorTopicYN="N">Male</DescriptorName>
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<MeshHeading><DescriptorName UI="D008875" MajorTopicYN="N">Middle Aged</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D011934" MajorTopicYN="Y">Reagent Strips</DescriptorName>
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<MeshHeading><DescriptorName UI="D020133" MajorTopicYN="N">Reverse Transcriptase Polymerase Chain Reaction</DescriptorName>
<QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D012680" MajorTopicYN="N">Sensitivity and Specificity</DescriptorName>
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<MeshHeading><DescriptorName UI="D055815" MajorTopicYN="N">Young Adult</DescriptorName>
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<name sortKey="Prus Kowalczuk, Wanda" sort="Prus Kowalczuk, Wanda" uniqKey="Prus Kowalczuk W" first="Wanda" last="Prus-Kowalczuk">Wanda Prus-Kowalczuk</name>
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