A clinical utility of a strip test for influenza A/B and comparison with detection by RT PCR.
Identifieur interne : 000760 ( PubMed/Corpus ); précédent : 000759; suivant : 000761A clinical utility of a strip test for influenza A/B and comparison with detection by RT PCR.
Auteurs : Maciej Miarka ; Andrzej Horban ; Henryka Maliszewska ; Przemysław Bili Ski ; Wanda Prus-KowalczukSource :
- Acta biochimica Polonica [ 1734-154X ] ; 2014.
English descriptors
- KwdEn :
- Adolescent, Adult, Aged, Aged, 80 and over, Child, Female, Humans, Influenza A Virus, H1N1 Subtype (isolation & purification), Influenza B virus (isolation & purification), Influenza, Human (diagnosis), Influenza, Human (virology), Male, Middle Aged, Reagent Strips, Reverse Transcriptase Polymerase Chain Reaction (methods), Sensitivity and Specificity, Young Adult.
- MESH :
- chemical : Reagent Strips.
- diagnosis : Influenza, Human.
- isolation & purification : Influenza A Virus, H1N1 Subtype, Influenza B virus.
- methods : Reverse Transcriptase Polymerase Chain Reaction.
- virology : Influenza, Human.
- Adolescent, Adult, Aged, Aged, 80 and over, Child, Female, Humans, Male, Middle Aged, Sensitivity and Specificity, Young Adult.
Abstract
In June 2009 the World Health Organization announced influenza pandemic caused by A/H1N1/v virus. It became crucial to recognize new cases of A/H1N1/v infection. An effective screening diagnostic procedure was needed for patients suffering from influenza-like symptoms for making an initial diagnosis and analyzing epidemiological pattern of infection. We used a strip test for influenza A/B as a screening diagnostic procedure for patients suffering from influenza-like symptoms for making an initial diagnosis. For comparison, RT PCR for detecting A/H1N1/v was performed. The aim of this study was to assess the efficacy and sensitivity of the strip test and its value for making initial diagnosis of influenza A/H1N1/v.
PubMed: 25210936
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">A clinical utility of a strip test for influenza A/B and comparison with detection by RT PCR.</title><author><name sortKey="Miarka, Maciej" sort="Miarka, Maciej" uniqKey="Miarka M" first="Maciej" last="Miarka">Maciej Miarka</name><affiliation><nlm:affiliation>Regional Infectious Diseases Hospital, Warsaw, Poland.</nlm:affiliation></affiliation></author><author><name sortKey="Horban, Andrzej" sort="Horban, Andrzej" uniqKey="Horban A" first="Andrzej" last="Horban">Andrzej Horban</name><affiliation><nlm:affiliation>Regional Infectious Diseases Hospital, Warsaw, Poland.</nlm:affiliation></affiliation></author><author><name sortKey="Maliszewska, Henryka" sort="Maliszewska, Henryka" uniqKey="Maliszewska H" first="Henryka" last="Maliszewska">Henryka Maliszewska</name><affiliation><nlm:affiliation>Regional Infectious Diseases Hospital, Warsaw, Poland.</nlm:affiliation></affiliation></author><author><name sortKey="Bili Ski, Przemyslaw" sort="Bili Ski, Przemyslaw" uniqKey="Bili Ski P" first="Przemysław" last="Bili Ski">Przemysław Bili Ski</name><affiliation><nlm:affiliation>Chief Sanitary Inspectorate, Warsaw, Poland.</nlm:affiliation></affiliation></author><author><name sortKey="Prus Kowalczuk, Wanda" sort="Prus Kowalczuk, Wanda" uniqKey="Prus Kowalczuk W" first="Wanda" last="Prus-Kowalczuk">Wanda Prus-Kowalczuk</name><affiliation><nlm:affiliation>Regional Infectious Diseases Hospital, Warsaw, Poland.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2014">2014</date><idno type="RBID">pubmed:25210936</idno><idno type="pmid">25210936</idno><idno type="wicri:Area/PubMed/Corpus">000760</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000760</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">A clinical utility of a strip test for influenza A/B and comparison with detection by RT PCR.</title><author><name sortKey="Miarka, Maciej" sort="Miarka, Maciej" uniqKey="Miarka M" first="Maciej" last="Miarka">Maciej Miarka</name><affiliation><nlm:affiliation>Regional Infectious Diseases Hospital, Warsaw, Poland.</nlm:affiliation></affiliation></author><author><name sortKey="Horban, Andrzej" sort="Horban, Andrzej" uniqKey="Horban A" first="Andrzej" last="Horban">Andrzej Horban</name><affiliation><nlm:affiliation>Regional Infectious Diseases Hospital, Warsaw, Poland.</nlm:affiliation></affiliation></author><author><name sortKey="Maliszewska, Henryka" sort="Maliszewska, Henryka" uniqKey="Maliszewska H" first="Henryka" last="Maliszewska">Henryka Maliszewska</name><affiliation><nlm:affiliation>Regional Infectious Diseases Hospital, Warsaw, Poland.</nlm:affiliation></affiliation></author><author><name sortKey="Bili Ski, Przemyslaw" sort="Bili Ski, Przemyslaw" uniqKey="Bili Ski P" first="Przemysław" last="Bili Ski">Przemysław Bili Ski</name><affiliation><nlm:affiliation>Chief Sanitary Inspectorate, Warsaw, Poland.</nlm:affiliation></affiliation></author><author><name sortKey="Prus Kowalczuk, Wanda" sort="Prus Kowalczuk, Wanda" uniqKey="Prus Kowalczuk W" first="Wanda" last="Prus-Kowalczuk">Wanda Prus-Kowalczuk</name><affiliation><nlm:affiliation>Regional Infectious Diseases Hospital, Warsaw, Poland.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Acta biochimica Polonica</title><idno type="eISSN">1734-154X</idno><imprint><date when="2014" type="published">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adolescent</term><term>Adult</term><term>Aged</term><term>Aged, 80 and over</term><term>Child</term><term>Female</term><term>Humans</term><term>Influenza A Virus, H1N1 Subtype (isolation & purification)</term><term>Influenza B virus (isolation & purification)</term><term>Influenza, Human (diagnosis)</term><term>Influenza, Human (virology)</term><term>Male</term><term>Middle Aged</term><term>Reagent Strips</term><term>Reverse Transcriptase Polymerase Chain Reaction (methods)</term><term>Sensitivity and Specificity</term><term>Young Adult</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Reagent Strips</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Influenza, Human</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Influenza A Virus, H1N1 Subtype</term><term>Influenza B virus</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Reverse Transcriptase Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Influenza, Human</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adolescent</term><term>Adult</term><term>Aged</term><term>Aged, 80 and over</term><term>Child</term><term>Female</term><term>Humans</term><term>Male</term><term>Middle Aged</term><term>Sensitivity and Specificity</term><term>Young Adult</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In June 2009 the World Health Organization announced influenza pandemic caused by A/H1N1/v virus. It became crucial to recognize new cases of A/H1N1/v infection. An effective screening diagnostic procedure was needed for patients suffering from influenza-like symptoms for making an initial diagnosis and analyzing epidemiological pattern of infection. We used a strip test for influenza A/B as a screening diagnostic procedure for patients suffering from influenza-like symptoms for making an initial diagnosis. For comparison, RT PCR for detecting A/H1N1/v was performed. The aim of this study was to assess the efficacy and sensitivity of the strip test and its value for making initial diagnosis of influenza A/H1N1/v.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">25210936</PMID><DateCompleted><Year>2014</Year><Month>12</Month><Day>31</Day></DateCompleted><DateRevised><Year>2014</Year><Month>10</Month><Day>02</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1734-154X</ISSN><JournalIssue CitedMedium="Internet"><Volume>61</Volume><Issue>3</Issue><PubDate><Year>2014</Year></PubDate></JournalIssue><Title>Acta biochimica Polonica</Title><ISOAbbreviation>Acta Biochim. Pol.</ISOAbbreviation></Journal><ArticleTitle>A clinical utility of a strip test for influenza A/B and comparison with detection by RT PCR.</ArticleTitle><Pagination><MedlinePgn>485-7</MedlinePgn></Pagination><Abstract><AbstractText Label="INTRODUCTION AND OBJECTIVE" NlmCategory="OBJECTIVE">In June 2009 the World Health Organization announced influenza pandemic caused by A/H1N1/v virus. It became crucial to recognize new cases of A/H1N1/v infection. An effective screening diagnostic procedure was needed for patients suffering from influenza-like symptoms for making an initial diagnosis and analyzing epidemiological pattern of infection. We used a strip test for influenza A/B as a screening diagnostic procedure for patients suffering from influenza-like symptoms for making an initial diagnosis. For comparison, RT PCR for detecting A/H1N1/v was performed. The aim of this study was to assess the efficacy and sensitivity of the strip test and its value for making initial diagnosis of influenza A/H1N1/v.</AbstractText><AbstractText Label="MATERIAL AND METHODS" NlmCategory="METHODS">Strip testing for the influenza A/B infection was performed on 1123 patients with influenza-like symptoms in the Admission Unit of the Regional Infectious Diseases Hospital in Warsaw. Strip test results were analyzed according to the age of patients and season of the year. For 97 patients strip test results for detecting A/H1N1 infection were compared with those obtained by RT PCR.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">There were no statistically significant differences found between the methods and strip testing demonstrated sensitivity of 61% and specificity of 71%.</AbstractText><AbstractText Label="CONCLUSIONS" NlmCategory="CONCLUSIONS">No statistically significant differences were found between the two methods, however, strip test had low sensitivity and specificity.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Miarka</LastName><ForeName>Maciej</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Regional Infectious Diseases Hospital, Warsaw, Poland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Horban</LastName><ForeName>Andrzej</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Regional Infectious Diseases Hospital, Warsaw, Poland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Maliszewska</LastName><ForeName>Henryka</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Regional Infectious Diseases Hospital, Warsaw, Poland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Biliński</LastName><ForeName>Przemysław</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>Chief Sanitary Inspectorate, Warsaw, Poland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Prus-Kowalczuk</LastName><ForeName>Wanda</ForeName><Initials>W</Initials><AffiliationInfo><Affiliation>Regional Infectious Diseases Hospital, Warsaw, Poland.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D003160">Comparative Study</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2014</Year><Month>09</Month><Day>12</Day></ArticleDate></Article><MedlineJournalInfo><Country>Poland</Country><MedlineTA>Acta Biochim Pol</MedlineTA><NlmUniqueID>14520300R</NlmUniqueID><ISSNLinking>0001-527X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011934">Reagent Strips</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000293" MajorTopicYN="N">Adolescent</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000328" MajorTopicYN="N">Adult</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000368" MajorTopicYN="N">Aged</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000369" MajorTopicYN="N">Aged, 80 and over</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002648" MajorTopicYN="N">Child</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005260" MajorTopicYN="N">Female</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D053118" MajorTopicYN="N">Influenza A Virus, H1N1 Subtype</DescriptorName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009981" MajorTopicYN="N">Influenza B virus</DescriptorName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D007251" MajorTopicYN="N">Influenza, Human</DescriptorName><QualifierName UI="Q000175" MajorTopicYN="Y">diagnosis</QualifierName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008297" MajorTopicYN="N">Male</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008875" MajorTopicYN="N">Middle Aged</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011934" MajorTopicYN="Y">Reagent Strips</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D020133" MajorTopicYN="N">Reverse Transcriptase Polymerase Chain Reaction</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012680" MajorTopicYN="N">Sensitivity and Specificity</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D055815" MajorTopicYN="N">Young Adult</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2014</Year><Month>08</Month><Day>31</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2014</Year><Month>09</Month><Day>01</Day></PubMedPubDate><PubMedPubDate 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