CpG Oligodeoxynucleotides Facilitate Delivery of Whole Inactivated H9N2 Influenza Virus via Transepithelial Dendrites of Dendritic Cells in Nasal Mucosa
Identifieur interne : 001998 ( Ncbi/Checkpoint ); précédent : 001997; suivant : 001999CpG Oligodeoxynucleotides Facilitate Delivery of Whole Inactivated H9N2 Influenza Virus via Transepithelial Dendrites of Dendritic Cells in Nasal Mucosa
Auteurs : Tao Qin ; Yinyan Yin ; Qinghua Yu ; Lulu Huang ; Xiaoqing Wang ; Jian Lin ; Qian YangSource :
- Journal of Virology [ 0022-538X ] ; 2015.
Descripteurs français
- KwdFr :
- Adjuvants immunologiques (administration et posologie), Administration par voie muqueuse, Animaux, Cellules dendritiques (immunologie), Cellules épithéliales (immunologie), Dendrites (immunologie), Mouvement cellulaire, Muqueuse nasale (immunologie), Noeuds lymphatiques (immunologie), Oligodésoxyribonucléotides (administration et posologie), Souris de lignée BALB C, Souris de lignée C57BL, Sous-type H9N2 du virus de la grippe A (immunologie), Vaccins antigrippaux (administration et posologie), Vaccins antigrippaux (immunologie), Vaccins inactivés (administration et posologie), Vaccins inactivés (immunologie).
- MESH :
- administration et posologie : Adjuvants immunologiques, Oligodésoxyribonucléotides, Vaccins antigrippaux, Vaccins inactivés.
- immunologie : Cellules dendritiques, Cellules épithéliales, Dendrites, Muqueuse nasale, Noeuds lymphatiques, Sous-type H9N2 du virus de la grippe A, Vaccins antigrippaux, Vaccins inactivés.
- Administration par voie muqueuse, Animaux, Mouvement cellulaire, Souris de lignée BALB C, Souris de lignée C57BL.
English descriptors
- KwdEn :
- Adjuvants, Immunologic (administration & dosage), Administration, Mucosal, Animals, Cell Movement, Dendrites (immunology), Dendritic Cells (immunology), Epithelial Cells (immunology), Influenza A Virus, H9N2 Subtype (immunology), Influenza Vaccines (administration & dosage), Influenza Vaccines (immunology), Lymph Nodes (immunology), Mice, Inbred BALB C, Mice, Inbred C57BL, Nasal Mucosa (immunology), Oligodeoxyribonucleotides (administration & dosage), Vaccines, Inactivated (administration & dosage), Vaccines, Inactivated (immunology).
- MESH :
- chemical , administration & dosage : Adjuvants, Immunologic, Influenza Vaccines, Oligodeoxyribonucleotides, Vaccines, Inactivated.
- immunology : Dendrites, Dendritic Cells, Epithelial Cells, Influenza A Virus, H9N2 Subtype, Influenza Vaccines, Lymph Nodes, Nasal Mucosa, Vaccines, Inactivated.
- Administration, Mucosal, Animals, Cell Movement, Mice, Inbred BALB C, Mice, Inbred C57BL.
Abstract
The spread of the low-pathogenicity avian H9N2 influenza virus has seriously increased the risk of a new influenza pandemic. Although whole inactivated virus (WIV) vaccine via intranasal pathway is the effective method of blocking virus transmission, the mucosal barrier seems to be a major factor hampering its development. CpG oligodeoxynucleotides, a known adjuvant, can target downstream dendritic cells (DCs) and effectively enhance the mucosal and systemic immune responses. However, the ability of CpGs to assist H9N2 WIV in transepithelial transport remains unknown. Here,
Url:
DOI: 10.1128/JVI.00296-15
PubMed: 25810544
PubMed Central: 4442437
Affiliations:
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PMC:4442437Le document en format XML
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<author><name sortKey="Yu, Qinghua" sort="Yu, Qinghua" uniqKey="Yu Q" first="Qinghua" last="Yu">Qinghua Yu</name>
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<author><name sortKey="Huang, Lulu" sort="Huang, Lulu" uniqKey="Huang L" first="Lulu" last="Huang">Lulu Huang</name>
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<author><name sortKey="Wang, Xiaoqing" sort="Wang, Xiaoqing" uniqKey="Wang X" first="Xiaoqing" last="Wang">Xiaoqing Wang</name>
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<author><name sortKey="Lin, Jian" sort="Lin, Jian" uniqKey="Lin J" first="Jian" last="Lin">Jian Lin</name>
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<author><name sortKey="Yang, Qian" sort="Yang, Qian" uniqKey="Yang Q" first="Qian" last="Yang">Qian Yang</name>
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<series><title level="j">Journal of Virology</title>
<idno type="ISSN">0022-538X</idno>
<idno type="eISSN">1098-5514</idno>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adjuvants, Immunologic (administration & dosage)</term>
<term>Administration, Mucosal</term>
<term>Animals</term>
<term>Cell Movement</term>
<term>Dendrites (immunology)</term>
<term>Dendritic Cells (immunology)</term>
<term>Epithelial Cells (immunology)</term>
<term>Influenza A Virus, H9N2 Subtype (immunology)</term>
<term>Influenza Vaccines (administration & dosage)</term>
<term>Influenza Vaccines (immunology)</term>
<term>Lymph Nodes (immunology)</term>
<term>Mice, Inbred BALB C</term>
<term>Mice, Inbred C57BL</term>
<term>Nasal Mucosa (immunology)</term>
<term>Oligodeoxyribonucleotides (administration & dosage)</term>
<term>Vaccines, Inactivated (administration & dosage)</term>
<term>Vaccines, Inactivated (immunology)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Adjuvants immunologiques (administration et posologie)</term>
<term>Administration par voie muqueuse</term>
<term>Animaux</term>
<term>Cellules dendritiques (immunologie)</term>
<term>Cellules épithéliales (immunologie)</term>
<term>Dendrites (immunologie)</term>
<term>Mouvement cellulaire</term>
<term>Muqueuse nasale (immunologie)</term>
<term>Noeuds lymphatiques (immunologie)</term>
<term>Oligodésoxyribonucléotides (administration et posologie)</term>
<term>Souris de lignée BALB C</term>
<term>Souris de lignée C57BL</term>
<term>Sous-type H9N2 du virus de la grippe A (immunologie)</term>
<term>Vaccins antigrippaux (administration et posologie)</term>
<term>Vaccins antigrippaux (immunologie)</term>
<term>Vaccins inactivés (administration et posologie)</term>
<term>Vaccins inactivés (immunologie)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="administration & dosage" xml:lang="en"><term>Adjuvants, Immunologic</term>
<term>Influenza Vaccines</term>
<term>Oligodeoxyribonucleotides</term>
<term>Vaccines, Inactivated</term>
</keywords>
<keywords scheme="MESH" qualifier="administration et posologie" xml:lang="fr"><term>Adjuvants immunologiques</term>
<term>Oligodésoxyribonucléotides</term>
<term>Vaccins antigrippaux</term>
<term>Vaccins inactivés</term>
</keywords>
<keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Cellules dendritiques</term>
<term>Cellules épithéliales</term>
<term>Dendrites</term>
<term>Muqueuse nasale</term>
<term>Noeuds lymphatiques</term>
<term>Sous-type H9N2 du virus de la grippe A</term>
<term>Vaccins antigrippaux</term>
<term>Vaccins inactivés</term>
</keywords>
<keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Dendrites</term>
<term>Dendritic Cells</term>
<term>Epithelial Cells</term>
<term>Influenza A Virus, H9N2 Subtype</term>
<term>Influenza Vaccines</term>
<term>Lymph Nodes</term>
<term>Nasal Mucosa</term>
<term>Vaccines, Inactivated</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Administration, Mucosal</term>
<term>Animals</term>
<term>Cell Movement</term>
<term>Mice, Inbred BALB C</term>
<term>Mice, Inbred C57BL</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Administration par voie muqueuse</term>
<term>Animaux</term>
<term>Mouvement cellulaire</term>
<term>Souris de lignée BALB C</term>
<term>Souris de lignée C57BL</term>
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<front><div type="abstract" xml:lang="en"><title>ABSTRACT</title>
<p>The spread of the low-pathogenicity avian H9N2 influenza virus has seriously increased the risk of a new influenza pandemic. Although whole inactivated virus (WIV) vaccine via intranasal pathway is the effective method of blocking virus transmission, the mucosal barrier seems to be a major factor hampering its development. CpG oligodeoxynucleotides, a known adjuvant, can target downstream dendritic cells (DCs) and effectively enhance the mucosal and systemic immune responses. However, the ability of CpGs to assist H9N2 WIV in transepithelial transport remains unknown. Here, <italic>in vitro</italic>
and <italic>in vivo</italic>
, we showed that CpGs provided assistance for H9N2 WIV in recruiting DCs to the nasal epithelial cells (ECs) and forming transepithelial dendrites (TEDs) to capture luminal viruses. CD103<sup>+</sup>
DCs participated in this process. Chemokine CCL20 from nasal ECs played a key role in driving DC recruitment and TED formation. Virus-loaded DCs quickly migrated into the draining cervical lymph nodes (CLNs) for antigen presentation. In addition, the competence of CpGs was independent of direct epithelial transport via the transcellular or paracellular pathway. Taken together, our data demonstrated that CpGs enhanced the transport of H9N2 WIV via TEDs of nasal DCs, which might be a novel mechanism for optimal adaptive immune responses.</p>
<p><bold>IMPORTANCE</bold>
This paper demonstrates by both an <italic>in vivo</italic>
and an <italic>in vitro</italic>
coculture model that CpG oligodeoxynucleotides, known as an adjuvant generally targeting downstream immune responses, also are crucial for the transport of H9N2 WIV across nasal epithelial cells (ECs) via the uptake of transepithelial dendrites (TEDs). Our results prove for the first time to our knowledge that the immune-potentiating mechanism of CpGs is based on strengthening the transepithelial uptake of H9N2 WIV in nasal mucosa. These findings provide a fresh perspective for further improvement of intranasal influenza vaccines, which are urgently needed in the face of the potential threat of H9N2 influenza.</p>
</div>
</front>
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<affiliations><list></list>
<tree><noCountry><name sortKey="Huang, Lulu" sort="Huang, Lulu" uniqKey="Huang L" first="Lulu" last="Huang">Lulu Huang</name>
<name sortKey="Lin, Jian" sort="Lin, Jian" uniqKey="Lin J" first="Jian" last="Lin">Jian Lin</name>
<name sortKey="Qin, Tao" sort="Qin, Tao" uniqKey="Qin T" first="Tao" last="Qin">Tao Qin</name>
<name sortKey="Wang, Xiaoqing" sort="Wang, Xiaoqing" uniqKey="Wang X" first="Xiaoqing" last="Wang">Xiaoqing Wang</name>
<name sortKey="Yang, Qian" sort="Yang, Qian" uniqKey="Yang Q" first="Qian" last="Yang">Qian Yang</name>
<name sortKey="Yin, Yinyan" sort="Yin, Yinyan" uniqKey="Yin Y" first="Yinyan" last="Yin">Yinyan Yin</name>
<name sortKey="Yu, Qinghua" sort="Yu, Qinghua" uniqKey="Yu Q" first="Qinghua" last="Yu">Qinghua Yu</name>
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