Visual detection of pandemic influenza A H1 N1 Virus 2009 by reverse-transcription loop-mediated isothermal amplification with hydroxynaphthol blue dye
Identifieur interne : 003835 ( Main/Merge ); précédent : 003834; suivant : 003836Visual detection of pandemic influenza A H1 N1 Virus 2009 by reverse-transcription loop-mediated isothermal amplification with hydroxynaphthol blue dye
Auteurs : Xue-Jun Ma [République populaire de Chine] ; Yue-Long Shu [République populaire de Chine] ; KAI NIE [République populaire de Chine] ; MENG QIN [République populaire de Chine] ; Da-Yan Wang [République populaire de Chine] ; Rong-Bao Gao [République populaire de Chine] ; MIAO WANG [République populaire de Chine] ; Le-Ying Wen [République populaire de Chine] ; FENG HAN [République populaire de Chine] ; Shu-Mei Zhou [République populaire de Chine] ; XIANG ZHAO [République populaire de Chine] ; Yan-Hui Cheng [République populaire de Chine] ; De-Xin Li [République populaire de Chine] ; Xiao-Ping Dong [République populaire de Chine]Source :
- Journal of virological methods [ 0166-0934 ] ; 2010.
Descripteurs français
- Pascal (Inist)
English descriptors
- KwdEn :
Abstract
A sensitive reverse-transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for rapid visual detection of pandemic infiuenza A H1N1 virus infection. The reaction was performed in one step in a single tube at 65 °C for 60 min with the addition of hydroxynaphthol blue (HNB) dye prior to amplification. The detection limit of the RT-LAMP assay was approximately 60 copies, and no cross-detection was observed. The assay was evaluated further with 50 clinical specimens diagnosed clinically with seasonal influenza or pandemic influenza A H1N1 virus infection. RT-LAMP with HNB dye was demonstrated to be a sensitive and easy assay for rapid detection of pandemic influenza A H1N1 virus.
Links toward previous steps (curation, corpus...)
- to stream PascalFrancis, to step Corpus: 001227
- to stream PascalFrancis, to step Curation: 000C42
- to stream PascalFrancis, to step Checkpoint: 000F03
Links to Exploration step
Pascal:10-0326822Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">Visual detection of pandemic influenza A H1 N1 Virus 2009 by reverse-transcription loop-mediated isothermal amplification with hydroxynaphthol blue dye</title><author><name sortKey="Ma, Xue Jun" sort="Ma, Xue Jun" uniqKey="Ma X" first="Xue-Jun" last="Ma">Xue-Jun Ma</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Ying-Xin Road 100</s1><s2>Xuan Wu District, Beijing, 100052</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Xuan Wu District, Beijing, 100052</wicri:noRegion></affiliation></author><author><name sortKey="Shu, Yue Long" sort="Shu, Yue Long" uniqKey="Shu Y" first="Yue-Long" last="Shu">Yue-Long Shu</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Ying-Xin Road 100</s1><s2>Xuan Wu District, Beijing, 100052</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Xuan Wu District, Beijing, 100052</wicri:noRegion></affiliation></author><author><name sortKey="Kai Nie" sort="Kai Nie" uniqKey="Kai Nie" last="Kai Nie">KAI NIE</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Ying-Xin Road 100</s1><s2>Xuan Wu District, Beijing, 100052</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Xuan Wu District, Beijing, 100052</wicri:noRegion></affiliation></author><author><name sortKey="Meng Qin" sort="Meng Qin" uniqKey="Meng Qin" last="Meng Qin">MENG QIN</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Ying-Xin Road 100</s1><s2>Xuan Wu District, Beijing, 100052</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Xuan Wu District, Beijing, 100052</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Da Yan" sort="Wang, Da Yan" uniqKey="Wang D" first="Da-Yan" last="Wang">Da-Yan Wang</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Ying-Xin Road 100</s1><s2>Xuan Wu District, Beijing, 100052</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Xuan Wu District, Beijing, 100052</wicri:noRegion></affiliation></author><author><name sortKey="Gao, Rong Bao" sort="Gao, Rong Bao" uniqKey="Gao R" first="Rong-Bao" last="Gao">Rong-Bao Gao</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Ying-Xin Road 100</s1><s2>Xuan Wu District, Beijing, 100052</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Xuan Wu District, Beijing, 100052</wicri:noRegion></affiliation></author><author><name sortKey="Miao Wang" sort="Miao Wang" uniqKey="Miao Wang" last="Miao Wang">MIAO WANG</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Ying-Xin Road 100</s1><s2>Xuan Wu District, Beijing, 100052</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Xuan Wu District, Beijing, 100052</wicri:noRegion></affiliation></author><author><name sortKey="Wen, Le Ying" sort="Wen, Le Ying" uniqKey="Wen L" first="Le-Ying" last="Wen">Le-Ying Wen</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Ying-Xin Road 100</s1><s2>Xuan Wu District, Beijing, 100052</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Xuan Wu District, Beijing, 100052</wicri:noRegion></affiliation></author><author><name sortKey="Feng Han" sort="Feng Han" uniqKey="Feng Han" last="Feng Han">FENG HAN</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Ying-Xin Road 100</s1><s2>Xuan Wu District, Beijing, 100052</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Xuan Wu District, Beijing, 100052</wicri:noRegion></affiliation></author><author><name sortKey="Zhou, Shu Mei" sort="Zhou, Shu Mei" uniqKey="Zhou S" first="Shu-Mei" last="Zhou">Shu-Mei Zhou</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Ying-Xin Road 100</s1><s2>Xuan Wu District, Beijing, 100052</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Xuan Wu District, Beijing, 100052</wicri:noRegion></affiliation></author><author><name sortKey="Xiang Zhao" sort="Xiang Zhao" uniqKey="Xiang Zhao" last="Xiang Zhao">XIANG ZHAO</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Ying-Xin Road 100</s1><s2>Xuan Wu District, Beijing, 100052</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Xuan Wu District, Beijing, 100052</wicri:noRegion></affiliation></author><author><name sortKey="Cheng, Yan Hui" sort="Cheng, Yan Hui" uniqKey="Cheng Y" first="Yan-Hui" last="Cheng">Yan-Hui Cheng</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Ying-Xin Road 100</s1><s2>Xuan Wu District, Beijing, 100052</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Xuan Wu District, Beijing, 100052</wicri:noRegion></affiliation></author><author><name sortKey="Li, De Xin" sort="Li, De Xin" uniqKey="Li D" first="De-Xin" last="Li">De-Xin Li</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Ying-Xin Road 100</s1><s2>Xuan Wu District, Beijing, 100052</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Xuan Wu District, Beijing, 100052</wicri:noRegion></affiliation></author><author><name sortKey="Dong, Xiao Ping" sort="Dong, Xiao Ping" uniqKey="Dong X" first="Xiao-Ping" last="Dong">Xiao-Ping Dong</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Ying-Xin Road 100</s1><s2>Xuan Wu District, Beijing, 100052</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Xuan Wu District, Beijing, 100052</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">INIST</idno><idno type="inist">10-0326822</idno><date when="2010">2010</date><idno type="stanalyst">PASCAL 10-0326822 INIST</idno><idno type="RBID">Pascal:10-0326822</idno><idno type="wicri:Area/PascalFrancis/Corpus">001227</idno><idno type="wicri:Area/PascalFrancis/Curation">000C42</idno><idno type="wicri:Area/PascalFrancis/Checkpoint">000F03</idno><idno type="wicri:explorRef" wicri:stream="PascalFrancis" wicri:step="Checkpoint">000F03</idno><idno type="wicri:doubleKey">0166-0934:2010:Ma X:visual:detection:of</idno><idno type="wicri:Area/Main/Merge">003835</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Visual detection of pandemic influenza A H1 N1 Virus 2009 by reverse-transcription loop-mediated isothermal amplification with hydroxynaphthol blue dye</title><author><name sortKey="Ma, Xue Jun" sort="Ma, Xue Jun" uniqKey="Ma X" first="Xue-Jun" last="Ma">Xue-Jun Ma</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Ying-Xin Road 100</s1><s2>Xuan Wu District, Beijing, 100052</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Xuan Wu District, Beijing, 100052</wicri:noRegion></affiliation></author><author><name sortKey="Shu, Yue Long" sort="Shu, Yue Long" uniqKey="Shu Y" first="Yue-Long" last="Shu">Yue-Long Shu</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Ying-Xin Road 100</s1><s2>Xuan Wu District, Beijing, 100052</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Xuan Wu District, Beijing, 100052</wicri:noRegion></affiliation></author><author><name sortKey="Kai Nie" sort="Kai Nie" uniqKey="Kai Nie" last="Kai Nie">KAI NIE</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Ying-Xin Road 100</s1><s2>Xuan Wu District, Beijing, 100052</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Xuan Wu District, Beijing, 100052</wicri:noRegion></affiliation></author><author><name sortKey="Meng Qin" sort="Meng Qin" uniqKey="Meng Qin" last="Meng Qin">MENG QIN</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Ying-Xin Road 100</s1><s2>Xuan Wu District, Beijing, 100052</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Xuan Wu District, Beijing, 100052</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Da Yan" sort="Wang, Da Yan" uniqKey="Wang D" first="Da-Yan" last="Wang">Da-Yan Wang</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Ying-Xin Road 100</s1><s2>Xuan Wu District, Beijing, 100052</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Xuan Wu District, Beijing, 100052</wicri:noRegion></affiliation></author><author><name sortKey="Gao, Rong Bao" sort="Gao, Rong Bao" uniqKey="Gao R" first="Rong-Bao" last="Gao">Rong-Bao Gao</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Ying-Xin Road 100</s1><s2>Xuan Wu District, Beijing, 100052</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Xuan Wu District, Beijing, 100052</wicri:noRegion></affiliation></author><author><name sortKey="Miao Wang" sort="Miao Wang" uniqKey="Miao Wang" last="Miao Wang">MIAO WANG</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Ying-Xin Road 100</s1><s2>Xuan Wu District, Beijing, 100052</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Xuan Wu District, Beijing, 100052</wicri:noRegion></affiliation></author><author><name sortKey="Wen, Le Ying" sort="Wen, Le Ying" uniqKey="Wen L" first="Le-Ying" last="Wen">Le-Ying Wen</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Ying-Xin Road 100</s1><s2>Xuan Wu District, Beijing, 100052</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Xuan Wu District, Beijing, 100052</wicri:noRegion></affiliation></author><author><name sortKey="Feng Han" sort="Feng Han" uniqKey="Feng Han" last="Feng Han">FENG HAN</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Ying-Xin Road 100</s1><s2>Xuan Wu District, Beijing, 100052</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Xuan Wu District, Beijing, 100052</wicri:noRegion></affiliation></author><author><name sortKey="Zhou, Shu Mei" sort="Zhou, Shu Mei" uniqKey="Zhou S" first="Shu-Mei" last="Zhou">Shu-Mei Zhou</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Ying-Xin Road 100</s1><s2>Xuan Wu District, Beijing, 100052</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Xuan Wu District, Beijing, 100052</wicri:noRegion></affiliation></author><author><name sortKey="Xiang Zhao" sort="Xiang Zhao" uniqKey="Xiang Zhao" last="Xiang Zhao">XIANG ZHAO</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Ying-Xin Road 100</s1><s2>Xuan Wu District, Beijing, 100052</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Xuan Wu District, Beijing, 100052</wicri:noRegion></affiliation></author><author><name sortKey="Cheng, Yan Hui" sort="Cheng, Yan Hui" uniqKey="Cheng Y" first="Yan-Hui" last="Cheng">Yan-Hui Cheng</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Ying-Xin Road 100</s1><s2>Xuan Wu District, Beijing, 100052</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Xuan Wu District, Beijing, 100052</wicri:noRegion></affiliation></author><author><name sortKey="Li, De Xin" sort="Li, De Xin" uniqKey="Li D" first="De-Xin" last="Li">De-Xin Li</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Ying-Xin Road 100</s1><s2>Xuan Wu District, Beijing, 100052</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Xuan Wu District, Beijing, 100052</wicri:noRegion></affiliation></author><author><name sortKey="Dong, Xiao Ping" sort="Dong, Xiao Ping" uniqKey="Dong X" first="Xiao-Ping" last="Dong">Xiao-Ping Dong</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Ying-Xin Road 100</s1><s2>Xuan Wu District, Beijing, 100052</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Xuan Wu District, Beijing, 100052</wicri:noRegion></affiliation></author></analytic><series><title level="j" type="main">Journal of virological methods</title><title level="j" type="abbreviated">J. virol. methods</title><idno type="ISSN">0166-0934</idno><imprint><date when="2010">2010</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Journal of virological methods</title><title level="j" type="abbreviated">J. virol. methods</title><idno type="ISSN">0166-0934</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Colorimetry</term><term>Detection</term><term>Influenza A virus</term><term>Influenzavirus A(H1N1)</term><term>Loop mediated isothermal amplification reaction</term><term>Method</term><term>Transcription</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Virus grippal A</term><term>Détection</term><term>Transcription</term><term>Colorimétrie</term><term>Méthode</term><term>Technique LAMP</term><term>Virus grippal A(H1N1)</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A sensitive reverse-transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for rapid visual detection of pandemic infiuenza A H1N1 virus infection. The reaction was performed in one step in a single tube at 65 °C for 60 min with the addition of hydroxynaphthol blue (HNB) dye prior to amplification. The detection limit of the RT-LAMP assay was approximately 60 copies, and no cross-detection was observed. The assay was evaluated further with 50 clinical specimens diagnosed clinically with seasonal influenza or pandemic influenza A H1N1 virus infection. RT-LAMP with HNB dye was demonstrated to be a sensitive and easy assay for rapid detection of pandemic influenza A H1N1 virus.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Ma, Xue Jun" sort="Ma, Xue Jun" uniqKey="Ma X" first="Xue-Jun" last="Ma">Xue-Jun Ma</name></noRegion><name sortKey="Cheng, Yan Hui" sort="Cheng, Yan Hui" uniqKey="Cheng Y" first="Yan-Hui" last="Cheng">Yan-Hui Cheng</name><name sortKey="Dong, Xiao Ping" sort="Dong, Xiao Ping" uniqKey="Dong X" first="Xiao-Ping" last="Dong">Xiao-Ping Dong</name><name sortKey="Feng Han" sort="Feng Han" uniqKey="Feng Han" last="Feng Han">FENG HAN</name><name sortKey="Gao, Rong Bao" sort="Gao, Rong Bao" uniqKey="Gao R" first="Rong-Bao" last="Gao">Rong-Bao Gao</name><name sortKey="Kai Nie" sort="Kai Nie" uniqKey="Kai Nie" last="Kai Nie">KAI NIE</name><name sortKey="Li, De Xin" sort="Li, De Xin" uniqKey="Li D" first="De-Xin" last="Li">De-Xin Li</name><name sortKey="Meng Qin" sort="Meng Qin" uniqKey="Meng Qin" last="Meng Qin">MENG QIN</name><name sortKey="Miao Wang" sort="Miao Wang" uniqKey="Miao Wang" last="Miao Wang">MIAO WANG</name><name sortKey="Shu, Yue Long" sort="Shu, Yue Long" uniqKey="Shu Y" first="Yue-Long" last="Shu">Yue-Long Shu</name><name sortKey="Wang, Da Yan" sort="Wang, Da Yan" uniqKey="Wang D" first="Da-Yan" last="Wang">Da-Yan Wang</name><name sortKey="Wen, Le Ying" sort="Wen, Le Ying" uniqKey="Wen L" first="Le-Ying" last="Wen">Le-Ying Wen</name><name sortKey="Xiang Zhao" sort="Xiang Zhao" uniqKey="Xiang Zhao" last="Xiang Zhao">XIANG ZHAO</name><name sortKey="Zhou, Shu Mei" sort="Zhou, Shu Mei" uniqKey="Zhou S" first="Shu-Mei" last="Zhou">Shu-Mei Zhou</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/PandemieGrippaleV1/Data/Main/Merge
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 003835 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Merge/biblio.hfd -nk 003835 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= PandemieGrippaleV1
|flux= Main
|étape= Merge
|type= RBID
|clé= Pascal:10-0326822
|texte= Visual detection of pandemic influenza A H1 N1 Virus 2009 by reverse-transcription loop-mediated isothermal amplification with hydroxynaphthol blue dye
}}
| This area was generated with Dilib version V0.6.34. |  |