Visual detection of pandemic influenza A H1 N1 Virus 2009 by reverse-transcription loop-mediated isothermal amplification with hydroxynaphthol blue dye
Identifieur interne : 000F03 ( PascalFrancis/Checkpoint ); précédent : 000F02; suivant : 000F04Visual detection of pandemic influenza A H1 N1 Virus 2009 by reverse-transcription loop-mediated isothermal amplification with hydroxynaphthol blue dye
Auteurs : Xue-Jun Ma [République populaire de Chine] ; Yue-Long Shu [République populaire de Chine] ; KAI NIE [République populaire de Chine] ; MENG QIN [République populaire de Chine] ; Da-Yan Wang [République populaire de Chine] ; Rong-Bao Gao [République populaire de Chine] ; MIAO WANG [République populaire de Chine] ; Le-Ying Wen [République populaire de Chine] ; FENG HAN [République populaire de Chine] ; Shu-Mei Zhou [République populaire de Chine] ; XIANG ZHAO [République populaire de Chine] ; Yan-Hui Cheng [République populaire de Chine] ; De-Xin Li [République populaire de Chine] ; Xiao-Ping Dong [République populaire de Chine]Source :
- Journal of virological methods [ 0166-0934 ] ; 2010.
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Abstract
A sensitive reverse-transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for rapid visual detection of pandemic infiuenza A H1N1 virus infection. The reaction was performed in one step in a single tube at 65 °C for 60 min with the addition of hydroxynaphthol blue (HNB) dye prior to amplification. The detection limit of the RT-LAMP assay was approximately 60 copies, and no cross-detection was observed. The assay was evaluated further with 50 clinical specimens diagnosed clinically with seasonal influenza or pandemic influenza A H1N1 virus infection. RT-LAMP with HNB dye was demonstrated to be a sensitive and easy assay for rapid detection of pandemic influenza A H1N1 virus.
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sort="Shu, Yue Long" uniqKey="Shu Y" first="Yue-Long" last="Shu">Yue-Long Shu</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Ying-Xin Road 100</s1><s2>Xuan Wu District, Beijing, 100052</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Xuan Wu District, Beijing, 100052</wicri:noRegion></affiliation></author><author><name sortKey="Kai Nie" sort="Kai Nie" uniqKey="Kai Nie" last="Kai Nie">KAI NIE</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Ying-Xin Road 100</s1><s2>Xuan Wu District, 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Chine</country><wicri:noRegion>Xuan Wu District, Beijing, 100052</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Da Yan" sort="Wang, Da Yan" uniqKey="Wang D" first="Da-Yan" last="Wang">Da-Yan Wang</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Ying-Xin Road 100</s1><s2>Xuan Wu District, Beijing, 100052</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Xuan Wu District, Beijing, 100052</wicri:noRegion></affiliation></author><author><name sortKey="Gao, Rong Bao" sort="Gao, Rong Bao" uniqKey="Gao R" first="Rong-Bao" last="Gao">Rong-Bao Gao</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Ying-Xin Road 100</s1><s2>Xuan Wu District, Beijing, 100052</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Xuan Wu District, Beijing, 100052</wicri:noRegion></affiliation></author><author><name sortKey="Miao Wang" sort="Miao Wang" uniqKey="Miao Wang" last="Miao Wang">MIAO WANG</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Ying-Xin Road 100</s1><s2>Xuan Wu District, Beijing, 100052</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 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100052</wicri:noRegion></affiliation></author><author><name sortKey="Feng Han" sort="Feng Han" uniqKey="Feng Han" last="Feng Han">FENG HAN</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Ying-Xin Road 100</s1><s2>Xuan Wu District, Beijing, 100052</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Xuan Wu District, Beijing, 100052</wicri:noRegion></affiliation></author><author><name sortKey="Zhou, Shu Mei" sort="Zhou, Shu Mei" uniqKey="Zhou S" first="Shu-Mei" last="Zhou">Shu-Mei Zhou</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Ying-Xin Road 100</s1><s2>Xuan Wu District, Beijing, 100052</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Xuan Wu District, Beijing, 100052</wicri:noRegion></affiliation></author><author><name sortKey="Xiang Zhao" sort="Xiang Zhao" uniqKey="Xiang Zhao" last="Xiang Zhao">XIANG ZHAO</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Ying-Xin Road 100</s1><s2>Xuan Wu District, Beijing, 100052</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 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uniqKey="Li D" first="De-Xin" last="Li">De-Xin Li</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Ying-Xin Road 100</s1><s2>Xuan Wu District, Beijing, 100052</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Xuan Wu District, Beijing, 100052</wicri:noRegion></affiliation></author><author><name sortKey="Dong, Xiao Ping" sort="Dong, Xiao Ping" uniqKey="Dong X" first="Xiao-Ping" last="Dong">Xiao-Ping Dong</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Ying-Xin Road 100</s1><s2>Xuan Wu District, Beijing, 100052</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Xuan Wu District, Beijing, 100052</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">INIST</idno><idno type="inist">10-0326822</idno><date when="2010">2010</date><idno type="stanalyst">PASCAL 10-0326822 INIST</idno><idno type="RBID">Pascal:10-0326822</idno><idno type="wicri:Area/PascalFrancis/Corpus">001227</idno><idno type="wicri:Area/PascalFrancis/Curation">000C42</idno><idno type="wicri:Area/PascalFrancis/Checkpoint">000F03</idno><idno type="wicri:explorRef" wicri:stream="PascalFrancis" wicri:step="Checkpoint">000F03</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Visual detection of pandemic influenza A H1 N1 Virus 2009 by reverse-transcription loop-mediated isothermal amplification with hydroxynaphthol blue dye</title><author><name sortKey="Ma, Xue Jun" sort="Ma, Xue Jun" uniqKey="Ma X" first="Xue-Jun" last="Ma">Xue-Jun Ma</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Ying-Xin Road 100</s1><s2>Xuan Wu District, Beijing, 100052</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Xuan Wu District, Beijing, 100052</wicri:noRegion></affiliation></author><author><name sortKey="Shu, Yue Long" sort="Shu, Yue Long" uniqKey="Shu Y" first="Yue-Long" last="Shu">Yue-Long Shu</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Ying-Xin Road 100</s1><s2>Xuan Wu District, Beijing, 100052</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Xuan Wu District, Beijing, 100052</wicri:noRegion></affiliation></author><author><name sortKey="Kai Nie" sort="Kai Nie" uniqKey="Kai Nie" last="Kai Nie">KAI NIE</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Ying-Xin Road 100</s1><s2>Xuan Wu District, Beijing, 100052</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Xuan Wu District, Beijing, 100052</wicri:noRegion></affiliation></author><author><name sortKey="Meng Qin" sort="Meng Qin" uniqKey="Meng Qin" last="Meng Qin">MENG QIN</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Ying-Xin Road 100</s1><s2>Xuan Wu District, Beijing, 100052</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Xuan Wu District, Beijing, 100052</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Da Yan" sort="Wang, Da Yan" uniqKey="Wang D" first="Da-Yan" last="Wang">Da-Yan Wang</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Ying-Xin Road 100</s1><s2>Xuan Wu District, Beijing, 100052</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Xuan Wu District, Beijing, 100052</wicri:noRegion></affiliation></author><author><name sortKey="Gao, Rong Bao" sort="Gao, Rong Bao" uniqKey="Gao R" first="Rong-Bao" last="Gao">Rong-Bao Gao</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Ying-Xin Road 100</s1><s2>Xuan Wu District, Beijing, 100052</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Xuan Wu District, Beijing, 100052</wicri:noRegion></affiliation></author><author><name sortKey="Miao Wang" sort="Miao Wang" uniqKey="Miao Wang" last="Miao Wang">MIAO WANG</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Ying-Xin Road 100</s1><s2>Xuan Wu District, Beijing, 100052</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Xuan Wu District, Beijing, 100052</wicri:noRegion></affiliation></author><author><name sortKey="Wen, Le Ying" sort="Wen, Le Ying" uniqKey="Wen L" first="Le-Ying" last="Wen">Le-Ying Wen</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Ying-Xin Road 100</s1><s2>Xuan Wu District, Beijing, 100052</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Xuan Wu District, Beijing, 100052</wicri:noRegion></affiliation></author><author><name sortKey="Feng Han" sort="Feng Han" uniqKey="Feng Han" last="Feng Han">FENG HAN</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Ying-Xin Road 100</s1><s2>Xuan Wu District, Beijing, 100052</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Xuan Wu District, Beijing, 100052</wicri:noRegion></affiliation></author><author><name sortKey="Zhou, Shu Mei" sort="Zhou, Shu Mei" uniqKey="Zhou S" first="Shu-Mei" last="Zhou">Shu-Mei Zhou</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Ying-Xin Road 100</s1><s2>Xuan Wu District, Beijing, 100052</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Xuan Wu District, Beijing, 100052</wicri:noRegion></affiliation></author><author><name sortKey="Xiang Zhao" sort="Xiang Zhao" uniqKey="Xiang Zhao" last="Xiang Zhao">XIANG ZHAO</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Ying-Xin Road 100</s1><s2>Xuan Wu District, Beijing, 100052</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Xuan Wu District, Beijing, 100052</wicri:noRegion></affiliation></author><author><name sortKey="Cheng, Yan Hui" sort="Cheng, Yan Hui" uniqKey="Cheng Y" first="Yan-Hui" last="Cheng">Yan-Hui Cheng</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Ying-Xin Road 100</s1><s2>Xuan Wu District, Beijing, 100052</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Xuan Wu District, Beijing, 100052</wicri:noRegion></affiliation></author><author><name sortKey="Li, De Xin" sort="Li, De Xin" uniqKey="Li D" first="De-Xin" last="Li">De-Xin Li</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Ying-Xin Road 100</s1><s2>Xuan Wu District, Beijing, 100052</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Xuan Wu District, Beijing, 100052</wicri:noRegion></affiliation></author><author><name sortKey="Dong, Xiao Ping" sort="Dong, Xiao Ping" uniqKey="Dong X" first="Xiao-Ping" last="Dong">Xiao-Ping Dong</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Ying-Xin Road 100</s1><s2>Xuan Wu District, Beijing, 100052</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Xuan Wu District, Beijing, 100052</wicri:noRegion></affiliation></author></analytic><series><title level="j" type="main">Journal of virological methods</title><title level="j" type="abbreviated">J. virol. methods</title><idno type="ISSN">0166-0934</idno><imprint><date when="2010">2010</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Journal of virological methods</title><title level="j" type="abbreviated">J. virol. methods</title><idno type="ISSN">0166-0934</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Colorimetry</term><term>Detection</term><term>Influenza A virus</term><term>Influenzavirus A(H1N1)</term><term>Loop mediated isothermal amplification reaction</term><term>Method</term><term>Transcription</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Virus grippal A</term><term>Détection</term><term>Transcription</term><term>Colorimétrie</term><term>Méthode</term><term>Technique LAMP</term><term>Virus grippal A(H1N1)</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A sensitive reverse-transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for rapid visual detection of pandemic infiuenza A H1N1 virus infection. The reaction was performed in one step in a single tube at 65 °C for 60 min with the addition of hydroxynaphthol blue (HNB) dye prior to amplification. The detection limit of the RT-LAMP assay was approximately 60 copies, and no cross-detection was observed. The assay was evaluated further with 50 clinical specimens diagnosed clinically with seasonal influenza or pandemic influenza A H1N1 virus infection. RT-LAMP with HNB dye was demonstrated to be a sensitive and easy assay for rapid detection of pandemic influenza A H1N1 virus.</div></front></TEI><inist><standard h6="B"><pA><fA01 i1="01" i2="1"><s0>0166-0934</s0></fA01><fA02 i1="01"><s0>JVMEDH</s0></fA02><fA03 i2="1"><s0>J. virol. methods</s0></fA03><fA05><s2>167</s2></fA05><fA06><s2>2</s2></fA06><fA08 i1="01" i2="1" l="ENG"><s1>Visual detection of pandemic influenza A H1 N1 Virus 2009 by reverse-transcription loop-mediated isothermal amplification with hydroxynaphthol blue dye</s1></fA08><fA11 i1="01" i2="1"><s1>MA (Xue-Jun)</s1></fA11><fA11 i1="02" i2="1"><s1>SHU (Yue-Long)</s1></fA11><fA11 i1="03" i2="1"><s1>KAI NIE</s1></fA11><fA11 i1="04" i2="1"><s1>MENG QIN</s1></fA11><fA11 i1="05" i2="1"><s1>WANG (Da-Yan)</s1></fA11><fA11 i1="06" i2="1"><s1>GAO (Rong-Bao)</s1></fA11><fA11 i1="07" i2="1"><s1>MIAO WANG</s1></fA11><fA11 i1="08" i2="1"><s1>WEN (Le-Ying)</s1></fA11><fA11 i1="09" i2="1"><s1>FENG HAN</s1></fA11><fA11 i1="10" i2="1"><s1>ZHOU (Shu-Mei)</s1></fA11><fA11 i1="11" i2="1"><s1>XIANG ZHAO</s1></fA11><fA11 i1="12" i2="1"><s1>CHENG (Yan-Hui)</s1></fA11><fA11 i1="13" i2="1"><s1>LI (De-Xin)</s1></fA11><fA11 i1="14" i2="1"><s1>DONG (Xiao-Ping)</s1></fA11><fA14 i1="01"><s1>National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Ying-Xin Road 100</s1><s2>Xuan Wu District, Beijing, 100052</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ></fA14><fA20><s1>214-217</s1></fA20><fA21><s1>2010</s1></fA21><fA23 i1="01"><s0>ENG</s0></fA23><fA43 i1="01"><s1>INIST</s1><s2>18295</s2><s5>354000192597880170</s5></fA43><fA44><s0>0000</s0><s1>© 2010 INIST-CNRS. All rights reserved.</s1></fA44><fA45><s0>1/4 p.</s0></fA45><fA47 i1="01" i2="1"><s0>10-0326822</s0></fA47><fA60><s1>P</s1><s3>CC</s3></fA60><fA61><s0>A</s0></fA61><fA64 i1="01" i2="1"><s0>Journal of virological methods</s0></fA64><fA66 i1="01"><s0>GBR</s0></fA66><fC01 i1="01" l="ENG"><s0>A sensitive reverse-transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for rapid visual detection of pandemic infiuenza A H1N1 virus infection. The reaction was performed in one step in a single tube at 65 °C for 60 min with the addition of hydroxynaphthol blue (HNB) dye prior to amplification. The detection limit of the RT-LAMP assay was approximately 60 copies, and no cross-detection was observed. The assay was evaluated further with 50 clinical specimens diagnosed clinically with seasonal influenza or pandemic influenza A H1N1 virus infection. RT-LAMP with HNB dye was demonstrated to be a sensitive and easy assay for rapid detection of pandemic influenza A H1N1 virus.</s0></fC01><fC02 i1="01" i2="X"><s0>002A05C09</s0></fC02><fC03 i1="01" i2="X" l="FRE"><s0>Virus grippal A</s0><s2>NW</s2><s5>01</s5></fC03><fC03 i1="01" i2="X" l="ENG"><s0>Influenza A virus</s0><s2>NW</s2><s5>01</s5></fC03><fC03 i1="01" i2="X" l="SPA"><s0>Influenza A virus</s0><s2>NW</s2><s5>01</s5></fC03><fC03 i1="02" i2="X" l="FRE"><s0>Détection</s0><s5>05</s5></fC03><fC03 i1="02" i2="X" l="ENG"><s0>Detection</s0><s5>05</s5></fC03><fC03 i1="02" i2="X" l="SPA"><s0>Detección</s0><s5>05</s5></fC03><fC03 i1="03" i2="X" l="FRE"><s0>Transcription</s0><s5>06</s5></fC03><fC03 i1="03" i2="X" l="ENG"><s0>Transcription</s0><s5>06</s5></fC03><fC03 i1="03" i2="X" l="SPA"><s0>Transcripción</s0><s5>06</s5></fC03><fC03 i1="04" i2="X" l="FRE"><s0>Colorimétrie</s0><s5>07</s5></fC03><fC03 i1="04" i2="X" l="ENG"><s0>Colorimetry</s0><s5>07</s5></fC03><fC03 i1="04" i2="X" l="SPA"><s0>Colorimetría</s0><s5>07</s5></fC03><fC03 i1="05" i2="X" l="FRE"><s0>Méthode</s0><s5>08</s5></fC03><fC03 i1="05" i2="X" l="ENG"><s0>Method</s0><s5>08</s5></fC03><fC03 i1="05" i2="X" l="SPA"><s0>Método</s0><s5>08</s5></fC03><fC03 i1="06" i2="X" l="FRE"><s0>Technique LAMP</s0><s4>CD</s4><s5>96</s5></fC03><fC03 i1="06" i2="X" l="ENG"><s0>Loop mediated isothermal amplification reaction</s0><s4>CD</s4><s5>96</s5></fC03><fC03 i1="07" i2="X" l="FRE"><s0>Virus grippal A(H1N1)</s0><s4>CD</s4><s5>97</s5></fC03><fC03 i1="07" i2="X" l="ENG"><s0>Influenzavirus A(H1N1)</s0><s4>CD</s4><s5>97</s5></fC03><fC07 i1="01" i2="X" l="FRE"><s0>Influenzavirus A</s0><s2>NW</s2></fC07><fC07 i1="01" i2="X" l="ENG"><s0>Influenzavirus A</s0><s2>NW</s2></fC07><fC07 i1="01" i2="X" l="SPA"><s0>Influenzavirus A</s0><s2>NW</s2></fC07><fC07 i1="02" i2="X" l="FRE"><s0>Orthomyxoviridae</s0><s2>NW</s2></fC07><fC07 i1="02" i2="X" l="ENG"><s0>Orthomyxoviridae</s0><s2>NW</s2></fC07><fC07 i1="02" i2="X" l="SPA"><s0>Orthomyxoviridae</s0><s2>NW</s2></fC07><fC07 i1="03" i2="X" l="FRE"><s0>Virus</s0><s2>NW</s2></fC07><fC07 i1="03" i2="X" l="ENG"><s0>Virus</s0><s2>NW</s2></fC07><fC07 i1="03" i2="X" l="SPA"><s0>Virus</s0><s2>NW</s2></fC07><fN21><s1>207</s1></fN21><fN44 i1="01"><s1>OTO</s1></fN44><fN82><s1>OTO</s1></fN82></pA></standard></inist><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Ma, Xue Jun" sort="Ma, Xue Jun" uniqKey="Ma X" first="Xue-Jun" last="Ma">Xue-Jun Ma</name></noRegion><name sortKey="Cheng, Yan Hui" sort="Cheng, Yan Hui" uniqKey="Cheng Y" first="Yan-Hui" last="Cheng">Yan-Hui Cheng</name><name sortKey="Dong, Xiao Ping" sort="Dong, Xiao Ping" uniqKey="Dong X" first="Xiao-Ping" last="Dong">Xiao-Ping Dong</name><name sortKey="Feng Han" sort="Feng Han" uniqKey="Feng Han" last="Feng Han">FENG HAN</name><name sortKey="Gao, Rong Bao" sort="Gao, Rong Bao" uniqKey="Gao R" first="Rong-Bao" last="Gao">Rong-Bao Gao</name><name sortKey="Kai Nie" sort="Kai Nie" uniqKey="Kai Nie" last="Kai Nie">KAI NIE</name><name sortKey="Li, De Xin" sort="Li, De Xin" uniqKey="Li D" first="De-Xin" last="Li">De-Xin Li</name><name sortKey="Meng Qin" sort="Meng Qin" uniqKey="Meng Qin" last="Meng Qin">MENG QIN</name><name sortKey="Miao Wang" sort="Miao Wang" uniqKey="Miao Wang" last="Miao Wang">MIAO WANG</name><name sortKey="Shu, Yue Long" sort="Shu, Yue Long" uniqKey="Shu Y" first="Yue-Long" last="Shu">Yue-Long Shu</name><name sortKey="Wang, Da Yan" sort="Wang, Da Yan" uniqKey="Wang D" first="Da-Yan" last="Wang">Da-Yan Wang</name><name sortKey="Wen, Le Ying" sort="Wen, Le Ying" uniqKey="Wen L" first="Le-Ying" last="Wen">Le-Ying Wen</name><name sortKey="Xiang Zhao" sort="Xiang Zhao" uniqKey="Xiang Zhao" last="Xiang Zhao">XIANG ZHAO</name><name sortKey="Zhou, Shu Mei" sort="Zhou, Shu Mei" uniqKey="Zhou S" first="Shu-Mei" last="Zhou">Shu-Mei Zhou</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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