Development of an enzyme‐linked immunoassay for the quantitation of influenza haemagglutinin: an alternative method to single radial immunodiffusion
Identifieur interne : 001B52 ( Main/Exploration ); précédent : 001B51; suivant : 001B53Development of an enzyme‐linked immunoassay for the quantitation of influenza haemagglutinin: an alternative method to single radial immunodiffusion
Auteurs : Jesse Bodle ; Erin E. Verity ; Chi Ong ; Kirsten Vandenberg ; Robert Shaw ; Ian G. Barr ; Steven RockmanSource :
- Influenza and Other Respiratory Viruses [ 1750-2640 ] ; 2012.
Descripteurs français
- KwdFr :
- MESH :
- analyse : Glycoprotéine hémagglutinine du virus influenza.
- isolement et purification : Anticorps monoclonaux.
- métabolisme : Horseradish peroxidase.
- normes : Vaccins antigrippaux.
- Humains, Immunodiffusion, Technologie pharmaceutique, Test ELISA, Vaccins antigrippaux.
English descriptors
- KwdEn :
- Antibodies, Monoclonal (isolation & purification), Enzyme-Linked Immunosorbent Assay (methods), Hemagglutinin Glycoproteins, Influenza Virus (analysis), Horseradish Peroxidase (metabolism), Humans, Immunodiffusion (methods), Influenza Vaccines (chemistry), Influenza Vaccines (standards), Technology, Pharmaceutical (methods).
- MESH :
- chemical , analysis : Hemagglutinin Glycoproteins, Influenza Virus.
- chemical , chemistry : Influenza Vaccines.
- chemical , isolation & purification : Antibodies, Monoclonal.
- chemical , metabolism : Horseradish Peroxidase.
- methods : Enzyme-Linked Immunosorbent Assay, Immunodiffusion, Technology, Pharmaceutical.
- chemical , standards : Influenza Vaccines.
- Humans.
Abstract
Url:
DOI: 10.1111/j.1750-2659.2012.00375.x
PubMed: 22583601
PubMed Central: 5780761
Affiliations:
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Le document en format XML
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antibodies, Monoclonal (isolation & purification)</term>
<term>Enzyme-Linked Immunosorbent Assay (methods)</term>
<term>Hemagglutinin Glycoproteins, Influenza Virus (analysis)</term>
<term>Horseradish Peroxidase (metabolism)</term>
<term>Humans</term>
<term>Immunodiffusion (methods)</term>
<term>Influenza Vaccines (chemistry)</term>
<term>Influenza Vaccines (standards)</term>
<term>Technology, Pharmaceutical (methods)</term>
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<keywords scheme="KwdFr" xml:lang="fr"><term>Anticorps monoclonaux (isolement et purification)</term>
<term>Glycoprotéine hémagglutinine du virus influenza (analyse)</term>
<term>Horseradish peroxidase (métabolisme)</term>
<term>Humains</term>
<term>Immunodiffusion ()</term>
<term>Technologie pharmaceutique ()</term>
<term>Test ELISA ()</term>
<term>Vaccins antigrippaux ()</term>
<term>Vaccins antigrippaux (normes)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Hemagglutinin Glycoproteins, Influenza Virus</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Influenza Vaccines</term>
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<keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Antibodies, Monoclonal</term>
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<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Horseradish Peroxidase</term>
</keywords>
<keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Glycoprotéine hémagglutinine du virus influenza</term>
</keywords>
<keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Anticorps monoclonaux</term>
</keywords>
<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Enzyme-Linked Immunosorbent Assay</term>
<term>Immunodiffusion</term>
<term>Technology, Pharmaceutical</term>
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<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Horseradish peroxidase</term>
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<front><div type="abstract" xml:lang="en"><p><italic>Please cite this paper as:</italic>
Bodle <italic>et al.</italic>
(2013) Development of an enzyme‐linked immunoassay for the quantitation of influenza haemagglutinin: an alternative method to single radial immunodiffusion. Influenza and Other Respiratory Viruses 7(2) 191–200.</p>
<p><bold>Background </bold>
The current method used to measure haemagglutinin (HA) content for influenza vaccine formulation, single radial immunodiffusion (SRID), is lengthy and relies on the availability of matched standardised homologous reagents. The 2009 influenza pandemic highlighted the need to develop alternate assays that are able to rapidly quantitate HA antigen for vaccine formulation.</p>
<p><bold>Objectives </bold>
The aim of this work was to develop an enzyme‐linked immunoassay (EIA) for the rapid quantitation of H1, H3, H5 and B influenza HA antigens.</p>
<p><bold>Methods </bold>
Monoclonal antibodies (mAbs) selected for haemagglutination inhibition (HAI) activity were conjugated with horseradish peroxidase and used to establish a capture–detection EIA for the quantitation of HA antigen. Results were compared with the appropriate reference SRID assays to investigate assay performance and utility.</p>
<p><bold>Results </bold>
Quantitation of HA antigen by EIA correlated well with current reference SRID assays. EIA results showed equivalent precision and exhibited a similar capacity to detect HA antigen in virus samples that had been used in either stability or splitting studies, or subjected to physical or chemical stresses. EIA exhibited greater sensitivity than SRID and has the potential to be used in high‐throughput applications.</p>
<p><bold>Conclusions </bold>
We demonstrated the utility of EIA as a suitable alternative to SRID for HA antigen quantitation and stability assessment. This approach would lead to earlier availability of both seasonal and pandemic vaccines, because of the extended cross‐reactivity of reagents.</p>
</div>
</front>
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<tree><noCountry><name sortKey="Barr, Ian G" sort="Barr, Ian G" uniqKey="Barr I" first="Ian G." last="Barr">Ian G. Barr</name>
<name sortKey="Bodle, Jesse" sort="Bodle, Jesse" uniqKey="Bodle J" first="Jesse" last="Bodle">Jesse Bodle</name>
<name sortKey="Ong, Chi" sort="Ong, Chi" uniqKey="Ong C" first="Chi" last="Ong">Chi Ong</name>
<name sortKey="Rockman, Steven" sort="Rockman, Steven" uniqKey="Rockman S" first="Steven" last="Rockman">Steven Rockman</name>
<name sortKey="Shaw, Robert" sort="Shaw, Robert" uniqKey="Shaw R" first="Robert" last="Shaw">Robert Shaw</name>
<name sortKey="Vandenberg, Kirsten" sort="Vandenberg, Kirsten" uniqKey="Vandenberg K" first="Kirsten" last="Vandenberg">Kirsten Vandenberg</name>
<name sortKey="Verity, Erin E" sort="Verity, Erin E" uniqKey="Verity E" first="Erin E." last="Verity">Erin E. Verity</name>
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