Incorporation of the influenza A virus NA segment into virions does not require cognate non-coding sequences.
Identifieur interne : 000618 ( Main/Curation ); précédent : 000617; suivant : 000619Incorporation of the influenza A virus NA segment into virions does not require cognate non-coding sequences.
Auteurs : Bernadette Crescenzo-Chaigne [France] ; Cyril V S. Barbezange [France] ; Stéphane Léandri [France] ; Camille Roquin [France] ; Camille Berthault [France] ; Sylvie Van Der Werf [France]Source :
- Scientific Reports [ 2045-2322 ] ; 2017-02-27.
English descriptors
- mix :
Abstract
For each influenza virus genome segment, the coding sequence is flanked by non-coding (NC) regions comprising shared, conserved sequences and specific, non-conserved sequences. The latter and adjacent parts of the coding sequence are involved in genome packaging, but the precise role of the non-conserved NC sequences is still unclear. The aim of this study is to better understand the role of the non-conserved non-coding sequences in the incorporation of the viral segments into virions. The NA-segment NC sequences were systematically replaced by those of the seven other segments. Recombinant viruses harbouring two segments with identical NC sequences were successfully rescued. Virus growth kinetics and serial passages were performed, and incorporation of the viral segments was tested by real-time RT-PCR. An initial virus growth deficiency correlated to a specific defect in NA segment incorporation. Upon serial passages, growth properties were restored. Sequencing revealed that the replacing 5'NC sequence length drove the type of mutations obtained. With sequences longer than the original, point mutations in the coding region with or without substitutions in the 3'NC region were detected. With shorter sequences, insertions were observed in the 5'NC region. Restoration of viral fitness was linked to restoration of the NA segment incorporation.
Url:
DOI: 10.1038/srep43462
Links toward previous steps (curation, corpus...)
- to stream Hal, to step Corpus: Pour aller vers cette notice dans l'étape Curation :000674
- to stream Hal, to step Curation: Pour aller vers cette notice dans l'étape Curation :000674
- to stream Hal, to step Checkpoint: Pour aller vers cette notice dans l'étape Curation :000276
- to stream Main, to step Merge: Pour aller vers cette notice dans l'étape Curation :000620
Links to Exploration step
Hal:pasteur-01570194Le document en format XML
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<author><name sortKey="Leandri, Stephane" sort="Leandri, Stephane" uniqKey="Leandri S" first="Stéphane" last="Léandri">Stéphane Léandri</name>
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<author><name sortKey="Roquin, Camille" sort="Roquin, Camille" uniqKey="Roquin C" first="Camille" last="Roquin">Camille Roquin</name>
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<author><name sortKey="Berthault, Camille" sort="Berthault, Camille" uniqKey="Berthault C" first="Camille" last="Berthault">Camille Berthault</name>
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<author><name sortKey="Van Der Werf, Sylvie" sort="Van Der Werf, Sylvie" uniqKey="Van Der Werf S" first="Sylvie" last="Van Der Werf">Sylvie Van Der Werf</name>
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</analytic>
<idno type="DOI">10.1038/srep43462</idno>
<series><title level="j">Scientific Reports</title>
<idno type="ISSN">2045-2322</idno>
<imprint><date type="datePub">2017-02-27</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="mix" xml:lang="en"><term>Influenza virus</term>
<term>Non-coding RNAs</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"> <p>For each influenza virus genome segment, the coding sequence is flanked by non-coding (NC) regions comprising shared, conserved sequences and specific, non-conserved sequences. The latter and adjacent parts of the coding sequence are involved in genome packaging, but the precise role of the non-conserved NC sequences is still unclear. The aim of this study is to better understand the role of the non-conserved non-coding sequences in the incorporation of the viral segments into virions. The NA-segment NC sequences were systematically replaced by those of the seven other segments. Recombinant viruses harbouring two segments with identical NC sequences were successfully rescued. Virus growth kinetics and serial passages were performed, and incorporation of the viral segments was tested by real-time RT-PCR. An initial virus growth deficiency correlated to a specific defect in NA segment incorporation. Upon serial passages, growth properties were restored. Sequencing revealed that the replacing 5'NC sequence length drove the type of mutations obtained. With sequences longer than the original, point mutations in the coding region with or without substitutions in the 3'NC region were detected. With shorter sequences, insertions were observed in the 5'NC region. Restoration of viral fitness was linked to restoration of the NA segment incorporation.</p>
</div>
</front>
</TEI>
</record>
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