Incorporation of the influenza A virus NA segment into virions does not require cognate non-coding sequences.
Identifieur interne : 000276 ( Hal/Checkpoint ); précédent : 000275; suivant : 000277Incorporation of the influenza A virus NA segment into virions does not require cognate non-coding sequences.
Auteurs : Bernadette Crescenzo-Chaigne [France] ; Cyril V S. Barbezange [France] ; Stéphane Léandri [France] ; Camille Roquin [France] ; Camille Berthault [France] ; Sylvie Van Der Werf [France]Source :
- Scientific Reports [ 2045-2322 ] ; 2017-02-27.
English descriptors
- mix :
Abstract
For each influenza virus genome segment, the coding sequence is flanked by non-coding (NC) regions comprising shared, conserved sequences and specific, non-conserved sequences. The latter and adjacent parts of the coding sequence are involved in genome packaging, but the precise role of the non-conserved NC sequences is still unclear. The aim of this study is to better understand the role of the non-conserved non-coding sequences in the incorporation of the viral segments into virions. The NA-segment NC sequences were systematically replaced by those of the seven other segments. Recombinant viruses harbouring two segments with identical NC sequences were successfully rescued. Virus growth kinetics and serial passages were performed, and incorporation of the viral segments was tested by real-time RT-PCR. An initial virus growth deficiency correlated to a specific defect in NA segment incorporation. Upon serial passages, growth properties were restored. Sequencing revealed that the replacing 5'NC sequence length drove the type of mutations obtained. With sequences longer than the original, point mutations in the coding region with or without substitutions in the 3'NC region were detected. With shorter sequences, insertions were observed in the 5'NC region. Restoration of viral fitness was linked to restoration of the NA segment incorporation.
Url:
DOI: 10.1038/srep43462
Links toward previous steps (curation, corpus...)
Links to Exploration step
Hal:pasteur-01570194Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="eng">Incorporation of the influenza A virus NA segment into virions does not require cognate non-coding sequences.</title><author><name sortKey="Crescenzo Chaigne, Bernadette" sort="Crescenzo Chaigne, Bernadette" uniqKey="Crescenzo Chaigne B" first="Bernadette" last="Crescenzo-Chaigne">Bernadette Crescenzo-Chaigne</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-417138" status="OLD"> <idno type="IdRef">235658278</idno> <idno type="RNSR">200818198A</idno> <orgName>Génétique Moléculaire des Virus à ARN - Molecular Genetics of RNA Viruses</orgName> <orgName type="acronym">GMV-ARN (UMR_3569 / U-Pasteur_2)</orgName> <date type="end">2019-12-31</date> <desc> <address> <addrLine>Département de Virologie, 25-28 rue du docteur Roux, 75724 Paris cedex 15</addrLine> <country key="FR"></country> </address> <ref type="url">https://research.pasteur.fr/en/team/molecular-genetics-of-rna-viruses/</ref> </desc> <listRelation> <relation name="UMR_3569" active="#struct-441569" type="direct"></relation> <relation name="U-Pasteur_2" active="#struct-300301" type="direct"></relation> <relation active="#struct-300027" type="direct"></relation> </listRelation> <tutelles><tutelle name="UMR_3569" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID"> <idno type="IdRef">02636817X</idno> <idno type="ISNI">0000000122597504</idno> <orgName>Centre National de la Recherche Scientifique</orgName> <orgName type="acronym">CNRS</orgName> <date type="start">1939-10-19</date> <desc> <address> <country key="FR"></country> </address> <ref type="url">http://www.cnrs.fr/</ref> </desc> </org></tutelle><tutelle name="U-Pasteur_2" active="#struct-300301" type="direct"><org type="institution" xml:id="struct-300301" status="OLD"> <idno type="ISNI">0000000121514068</idno> <idno type="IdRef">027542084</idno> <orgName>Université Paris Diderot - Paris 7</orgName> <orgName type="acronym">UPD7</orgName> <date type="end">2019-12-31</date> <desc> <address> <addrLine>5 rue Thomas-Mann - 75205 Paris cedex 13</addrLine> <country key="FR"></country> </address> <ref type="url">http://www.univ-paris-diderot.fr</ref> </desc> </org></tutelle><tutelle active="#struct-300027" type="direct"><org type="institution" xml:id="struct-300027" status="VALID"> <idno type="IdRef">027936643</idno> <idno type="ISNI">0000 0001 2353 6535</idno> <orgName>Institut Pasteur [Paris]</orgName> <date type="start">1887-06-04</date> <desc> <address> <addrLine>25-28, rue du docteur Roux, 75724 Paris cedex 15</addrLine> <country key="FR"></country> </address> <ref type="url">https://www.pasteur.fr</ref> </desc> </org></tutelle></tutelles></hal:affiliation><country>France</country></affiliation></author><author><name sortKey="Barbezange, Cyril V S" sort="Barbezange, Cyril V S" uniqKey="Barbezange C" first="Cyril V S" last="Barbezange">Cyril V S. Barbezange</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-417138" status="OLD"> <idno type="IdRef">235658278</idno> <idno type="RNSR">200818198A</idno> <orgName>Génétique Moléculaire des Virus à ARN - Molecular Genetics of RNA Viruses</orgName> <orgName type="acronym">GMV-ARN (UMR_3569 / U-Pasteur_2)</orgName> <date type="end">2019-12-31</date> <desc> <address> <addrLine>Département de Virologie, 25-28 rue du docteur Roux, 75724 Paris cedex 15</addrLine> <country key="FR"></country> </address> <ref type="url">https://research.pasteur.fr/en/team/molecular-genetics-of-rna-viruses/</ref> </desc> <listRelation> <relation name="UMR_3569" active="#struct-441569" type="direct"></relation> <relation name="U-Pasteur_2" active="#struct-300301" type="direct"></relation> <relation active="#struct-300027" type="direct"></relation> </listRelation> <tutelles><tutelle name="UMR_3569" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID"> <idno type="IdRef">02636817X</idno> <idno type="ISNI">0000000122597504</idno> <orgName>Centre National de la Recherche Scientifique</orgName> <orgName type="acronym">CNRS</orgName> <date type="start">1939-10-19</date> <desc> <address> <country key="FR"></country> </address> <ref type="url">http://www.cnrs.fr/</ref> </desc> </org></tutelle><tutelle name="U-Pasteur_2" active="#struct-300301" type="direct"><org type="institution" xml:id="struct-300301" status="OLD"> <idno type="ISNI">0000000121514068</idno> <idno type="IdRef">027542084</idno> <orgName>Université Paris Diderot - Paris 7</orgName> <orgName type="acronym">UPD7</orgName> <date type="end">2019-12-31</date> <desc> <address> <addrLine>5 rue Thomas-Mann - 75205 Paris cedex 13</addrLine> <country key="FR"></country> </address> <ref type="url">http://www.univ-paris-diderot.fr</ref> </desc> </org></tutelle><tutelle active="#struct-300027" type="direct"><org type="institution" xml:id="struct-300027" status="VALID"> <idno type="IdRef">027936643</idno> <idno type="ISNI">0000 0001 2353 6535</idno> <orgName>Institut Pasteur [Paris]</orgName> <date type="start">1887-06-04</date> <desc> <address> <addrLine>25-28, rue du docteur Roux, 75724 Paris cedex 15</addrLine> <country key="FR"></country> </address> <ref type="url">https://www.pasteur.fr</ref> </desc> </org></tutelle></tutelles></hal:affiliation><country>France</country></affiliation></author><author><name sortKey="Leandri, Stephane" sort="Leandri, Stephane" uniqKey="Leandri S" first="Stéphane" last="Léandri">Stéphane Léandri</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-417138" status="OLD"> <idno type="IdRef">235658278</idno> <idno type="RNSR">200818198A</idno> <orgName>Génétique Moléculaire des Virus à ARN - Molecular Genetics of RNA Viruses</orgName> <orgName type="acronym">GMV-ARN (UMR_3569 / U-Pasteur_2)</orgName> <date type="end">2019-12-31</date> <desc> <address> <addrLine>Département de Virologie, 25-28 rue du docteur Roux, 75724 Paris cedex 15</addrLine> <country key="FR"></country> </address> <ref type="url">https://research.pasteur.fr/en/team/molecular-genetics-of-rna-viruses/</ref> </desc> <listRelation> <relation name="UMR_3569" active="#struct-441569" type="direct"></relation> <relation name="U-Pasteur_2" active="#struct-300301" type="direct"></relation> <relation active="#struct-300027" type="direct"></relation> </listRelation> <tutelles><tutelle name="UMR_3569" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID"> <idno type="IdRef">02636817X</idno> <idno type="ISNI">0000000122597504</idno> <orgName>Centre National de la Recherche Scientifique</orgName> <orgName type="acronym">CNRS</orgName> <date type="start">1939-10-19</date> <desc> <address> <country key="FR"></country> </address> <ref type="url">http://www.cnrs.fr/</ref> </desc> </org></tutelle><tutelle name="U-Pasteur_2" active="#struct-300301" type="direct"><org type="institution" xml:id="struct-300301" status="OLD"> <idno type="ISNI">0000000121514068</idno> <idno type="IdRef">027542084</idno> <orgName>Université Paris Diderot - Paris 7</orgName> <orgName type="acronym">UPD7</orgName> <date type="end">2019-12-31</date> <desc> <address> <addrLine>5 rue Thomas-Mann - 75205 Paris cedex 13</addrLine> <country key="FR"></country> </address> <ref type="url">http://www.univ-paris-diderot.fr</ref> </desc> </org></tutelle><tutelle active="#struct-300027" type="direct"><org type="institution" xml:id="struct-300027" status="VALID"> <idno type="IdRef">027936643</idno> <idno type="ISNI">0000 0001 2353 6535</idno> <orgName>Institut Pasteur [Paris]</orgName> <date type="start">1887-06-04</date> <desc> <address> <addrLine>25-28, rue du docteur Roux, 75724 Paris cedex 15</addrLine> <country key="FR"></country> </address> <ref type="url">https://www.pasteur.fr</ref> </desc> </org></tutelle></tutelles></hal:affiliation><country>France</country></affiliation></author><author><name sortKey="Roquin, Camille" sort="Roquin, Camille" uniqKey="Roquin C" first="Camille" last="Roquin">Camille Roquin</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-417138" status="OLD"> <idno type="IdRef">235658278</idno> <idno type="RNSR">200818198A</idno> <orgName>Génétique Moléculaire des Virus à ARN - Molecular Genetics of RNA Viruses</orgName> <orgName type="acronym">GMV-ARN (UMR_3569 / U-Pasteur_2)</orgName> <date type="end">2019-12-31</date> <desc> <address> <addrLine>Département de Virologie, 25-28 rue du docteur Roux, 75724 Paris cedex 15</addrLine> <country key="FR"></country> </address> <ref type="url">https://research.pasteur.fr/en/team/molecular-genetics-of-rna-viruses/</ref> </desc> <listRelation> <relation name="UMR_3569" active="#struct-441569" type="direct"></relation> <relation name="U-Pasteur_2" active="#struct-300301" type="direct"></relation> <relation active="#struct-300027" type="direct"></relation> </listRelation> <tutelles><tutelle name="UMR_3569" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID"> <idno type="IdRef">02636817X</idno> <idno type="ISNI">0000000122597504</idno> <orgName>Centre National de la Recherche Scientifique</orgName> <orgName type="acronym">CNRS</orgName> <date type="start">1939-10-19</date> <desc> <address> <country key="FR"></country> </address> <ref type="url">http://www.cnrs.fr/</ref> </desc> </org></tutelle><tutelle name="U-Pasteur_2" active="#struct-300301" type="direct"><org type="institution" xml:id="struct-300301" status="OLD"> <idno type="ISNI">0000000121514068</idno> <idno type="IdRef">027542084</idno> <orgName>Université Paris Diderot - Paris 7</orgName> <orgName type="acronym">UPD7</orgName> <date type="end">2019-12-31</date> <desc> <address> <addrLine>5 rue Thomas-Mann - 75205 Paris cedex 13</addrLine> <country key="FR"></country> </address> <ref type="url">http://www.univ-paris-diderot.fr</ref> </desc> </org></tutelle><tutelle active="#struct-300027" type="direct"><org type="institution" xml:id="struct-300027" status="VALID"> <idno type="IdRef">027936643</idno> <idno type="ISNI">0000 0001 2353 6535</idno> <orgName>Institut Pasteur [Paris]</orgName> <date type="start">1887-06-04</date> <desc> <address> <addrLine>25-28, rue du docteur Roux, 75724 Paris cedex 15</addrLine> <country key="FR"></country> </address> <ref type="url">https://www.pasteur.fr</ref> </desc> </org></tutelle></tutelles></hal:affiliation><country>France</country></affiliation></author><author><name sortKey="Berthault, Camille" sort="Berthault, Camille" uniqKey="Berthault C" first="Camille" last="Berthault">Camille Berthault</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-417138" status="OLD"> <idno type="IdRef">235658278</idno> <idno type="RNSR">200818198A</idno> <orgName>Génétique Moléculaire des Virus à ARN - Molecular Genetics of RNA Viruses</orgName> <orgName type="acronym">GMV-ARN (UMR_3569 / U-Pasteur_2)</orgName> <date type="end">2019-12-31</date> <desc> <address> <addrLine>Département de Virologie, 25-28 rue du docteur Roux, 75724 Paris cedex 15</addrLine> <country key="FR"></country> </address> <ref type="url">https://research.pasteur.fr/en/team/molecular-genetics-of-rna-viruses/</ref> </desc> <listRelation> <relation name="UMR_3569" active="#struct-441569" type="direct"></relation> <relation name="U-Pasteur_2" active="#struct-300301" type="direct"></relation> <relation active="#struct-300027" type="direct"></relation> </listRelation> <tutelles><tutelle name="UMR_3569" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID"> <idno type="IdRef">02636817X</idno> <idno type="ISNI">0000000122597504</idno> <orgName>Centre National de la Recherche Scientifique</orgName> <orgName type="acronym">CNRS</orgName> <date type="start">1939-10-19</date> <desc> <address> <country key="FR"></country> </address> <ref type="url">http://www.cnrs.fr/</ref> </desc> </org></tutelle><tutelle name="U-Pasteur_2" active="#struct-300301" type="direct"><org type="institution" xml:id="struct-300301" status="OLD"> <idno type="ISNI">0000000121514068</idno> <idno type="IdRef">027542084</idno> <orgName>Université Paris Diderot - Paris 7</orgName> <orgName type="acronym">UPD7</orgName> <date type="end">2019-12-31</date> <desc> <address> <addrLine>5 rue Thomas-Mann - 75205 Paris cedex 13</addrLine> <country key="FR"></country> </address> <ref type="url">http://www.univ-paris-diderot.fr</ref> </desc> </org></tutelle><tutelle active="#struct-300027" type="direct"><org type="institution" xml:id="struct-300027" status="VALID"> <idno type="IdRef">027936643</idno> <idno type="ISNI">0000 0001 2353 6535</idno> <orgName>Institut Pasteur [Paris]</orgName> <date type="start">1887-06-04</date> <desc> <address> <addrLine>25-28, rue du docteur Roux, 75724 Paris cedex 15</addrLine> <country key="FR"></country> </address> <ref type="url">https://www.pasteur.fr</ref> </desc> </org></tutelle></tutelles></hal:affiliation><country>France</country></affiliation></author><author><name sortKey="Van Der Werf, Sylvie" sort="Van Der Werf, Sylvie" uniqKey="Van Der Werf S" first="Sylvie" last="Van Der Werf">Sylvie Van Der Werf</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-417138" status="OLD"> <idno type="IdRef">235658278</idno> <idno type="RNSR">200818198A</idno> <orgName>Génétique Moléculaire des Virus à ARN - Molecular Genetics of RNA Viruses</orgName> <orgName type="acronym">GMV-ARN (UMR_3569 / U-Pasteur_2)</orgName> <date type="end">2019-12-31</date> <desc> <address> <addrLine>Département de Virologie, 25-28 rue du docteur Roux, 75724 Paris cedex 15</addrLine> <country key="FR"></country> </address> <ref type="url">https://research.pasteur.fr/en/team/molecular-genetics-of-rna-viruses/</ref> </desc> <listRelation> <relation name="UMR_3569" active="#struct-441569" type="direct"></relation> <relation name="U-Pasteur_2" active="#struct-300301" type="direct"></relation> <relation active="#struct-300027" type="direct"></relation> </listRelation> <tutelles><tutelle name="UMR_3569" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID"> <idno type="IdRef">02636817X</idno> <idno type="ISNI">0000000122597504</idno> <orgName>Centre National de la Recherche Scientifique</orgName> <orgName type="acronym">CNRS</orgName> <date type="start">1939-10-19</date> <desc> <address> <country key="FR"></country> </address> <ref type="url">http://www.cnrs.fr/</ref> </desc> </org></tutelle><tutelle name="U-Pasteur_2" active="#struct-300301" type="direct"><org type="institution" xml:id="struct-300301" status="OLD"> <idno type="ISNI">0000000121514068</idno> <idno type="IdRef">027542084</idno> <orgName>Université Paris Diderot - Paris 7</orgName> <orgName type="acronym">UPD7</orgName> <date type="end">2019-12-31</date> <desc> <address> <addrLine>5 rue Thomas-Mann - 75205 Paris cedex 13</addrLine> <country key="FR"></country> </address> <ref type="url">http://www.univ-paris-diderot.fr</ref> </desc> </org></tutelle><tutelle active="#struct-300027" type="direct"><org type="institution" xml:id="struct-300027" status="VALID"> <idno type="IdRef">027936643</idno> <idno type="ISNI">0000 0001 2353 6535</idno> <orgName>Institut Pasteur [Paris]</orgName> <date type="start">1887-06-04</date> <desc> <address> <addrLine>25-28, rue du docteur Roux, 75724 Paris cedex 15</addrLine> <country key="FR"></country> </address> <ref type="url">https://www.pasteur.fr</ref> </desc> </org></tutelle></tutelles></hal:affiliation><country>France</country></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">HAL</idno><idno type="RBID">Hal:pasteur-01570194</idno><idno type="halId">pasteur-01570194</idno><idno type="halUri">https://hal-pasteur.archives-ouvertes.fr/pasteur-01570194</idno><idno type="url">https://hal-pasteur.archives-ouvertes.fr/pasteur-01570194</idno><idno type="doi">10.1038/srep43462</idno><date when="2017-02-27">2017-02-27</date><idno type="wicri:Area/Hal/Corpus">000674</idno><idno type="wicri:Area/Hal/Curation">000674</idno><idno type="wicri:Area/Hal/Checkpoint">000276</idno><idno type="wicri:explorRef" wicri:stream="Hal" wicri:step="Checkpoint">000276</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="eng">Incorporation of the influenza A virus NA segment into virions does not require cognate non-coding sequences.</title><author><name sortKey="Crescenzo Chaigne, Bernadette" sort="Crescenzo Chaigne, Bernadette" uniqKey="Crescenzo Chaigne B" first="Bernadette" last="Crescenzo-Chaigne">Bernadette Crescenzo-Chaigne</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-417138" status="OLD"> <idno type="IdRef">235658278</idno> <idno type="RNSR">200818198A</idno> <orgName>Génétique Moléculaire des Virus à ARN - Molecular Genetics of RNA Viruses</orgName> <orgName type="acronym">GMV-ARN (UMR_3569 / U-Pasteur_2)</orgName> <date type="end">2019-12-31</date> <desc> <address> <addrLine>Département de Virologie, 25-28 rue du docteur Roux, 75724 Paris cedex 15</addrLine> <country key="FR"></country> </address> <ref type="url">https://research.pasteur.fr/en/team/molecular-genetics-of-rna-viruses/</ref> </desc> <listRelation> <relation name="UMR_3569" active="#struct-441569" type="direct"></relation> <relation name="U-Pasteur_2" active="#struct-300301" type="direct"></relation> <relation active="#struct-300027" type="direct"></relation> </listRelation> <tutelles><tutelle name="UMR_3569" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID"> <idno type="IdRef">02636817X</idno> <idno type="ISNI">0000000122597504</idno> <orgName>Centre National de la Recherche Scientifique</orgName> <orgName type="acronym">CNRS</orgName> <date type="start">1939-10-19</date> <desc> <address> <country key="FR"></country> </address> <ref type="url">http://www.cnrs.fr/</ref> </desc> </org></tutelle><tutelle name="U-Pasteur_2" active="#struct-300301" type="direct"><org type="institution" xml:id="struct-300301" status="OLD"> <idno type="ISNI">0000000121514068</idno> <idno type="IdRef">027542084</idno> <orgName>Université Paris Diderot - Paris 7</orgName> <orgName type="acronym">UPD7</orgName> <date type="end">2019-12-31</date> <desc> <address> <addrLine>5 rue Thomas-Mann - 75205 Paris cedex 13</addrLine> <country key="FR"></country> </address> <ref type="url">http://www.univ-paris-diderot.fr</ref> </desc> </org></tutelle><tutelle active="#struct-300027" type="direct"><org type="institution" xml:id="struct-300027" status="VALID"> <idno type="IdRef">027936643</idno> <idno type="ISNI">0000 0001 2353 6535</idno> <orgName>Institut Pasteur [Paris]</orgName> <date type="start">1887-06-04</date> <desc> <address> <addrLine>25-28, rue du docteur Roux, 75724 Paris cedex 15</addrLine> <country key="FR"></country> </address> <ref type="url">https://www.pasteur.fr</ref> </desc> </org></tutelle></tutelles></hal:affiliation><country>France</country></affiliation></author><author><name sortKey="Barbezange, Cyril V S" sort="Barbezange, Cyril V S" uniqKey="Barbezange C" first="Cyril V S" last="Barbezange">Cyril V S. Barbezange</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-417138" status="OLD"> <idno type="IdRef">235658278</idno> <idno type="RNSR">200818198A</idno> <orgName>Génétique Moléculaire des Virus à ARN - Molecular Genetics of RNA Viruses</orgName> <orgName type="acronym">GMV-ARN (UMR_3569 / U-Pasteur_2)</orgName> <date type="end">2019-12-31</date> <desc> <address> <addrLine>Département de Virologie, 25-28 rue du docteur Roux, 75724 Paris cedex 15</addrLine> <country key="FR"></country> </address> <ref type="url">https://research.pasteur.fr/en/team/molecular-genetics-of-rna-viruses/</ref> </desc> <listRelation> <relation name="UMR_3569" active="#struct-441569" type="direct"></relation> <relation name="U-Pasteur_2" active="#struct-300301" type="direct"></relation> <relation active="#struct-300027" type="direct"></relation> </listRelation> <tutelles><tutelle name="UMR_3569" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID"> <idno type="IdRef">02636817X</idno> <idno type="ISNI">0000000122597504</idno> <orgName>Centre National de la Recherche Scientifique</orgName> <orgName type="acronym">CNRS</orgName> <date type="start">1939-10-19</date> <desc> <address> <country key="FR"></country> </address> <ref type="url">http://www.cnrs.fr/</ref> </desc> </org></tutelle><tutelle name="U-Pasteur_2" active="#struct-300301" type="direct"><org type="institution" xml:id="struct-300301" status="OLD"> <idno type="ISNI">0000000121514068</idno> <idno type="IdRef">027542084</idno> <orgName>Université Paris Diderot - Paris 7</orgName> <orgName type="acronym">UPD7</orgName> <date type="end">2019-12-31</date> <desc> <address> <addrLine>5 rue Thomas-Mann - 75205 Paris cedex 13</addrLine> <country key="FR"></country> </address> <ref type="url">http://www.univ-paris-diderot.fr</ref> </desc> </org></tutelle><tutelle active="#struct-300027" type="direct"><org type="institution" xml:id="struct-300027" status="VALID"> <idno type="IdRef">027936643</idno> <idno type="ISNI">0000 0001 2353 6535</idno> <orgName>Institut Pasteur [Paris]</orgName> <date type="start">1887-06-04</date> <desc> <address> <addrLine>25-28, rue du docteur Roux, 75724 Paris cedex 15</addrLine> <country key="FR"></country> </address> <ref type="url">https://www.pasteur.fr</ref> </desc> </org></tutelle></tutelles></hal:affiliation><country>France</country></affiliation></author><author><name sortKey="Leandri, Stephane" sort="Leandri, Stephane" uniqKey="Leandri S" first="Stéphane" last="Léandri">Stéphane Léandri</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-417138" status="OLD"> <idno type="IdRef">235658278</idno> <idno type="RNSR">200818198A</idno> <orgName>Génétique Moléculaire des Virus à ARN - Molecular Genetics of RNA Viruses</orgName> <orgName type="acronym">GMV-ARN (UMR_3569 / U-Pasteur_2)</orgName> <date type="end">2019-12-31</date> <desc> <address> <addrLine>Département de Virologie, 25-28 rue du docteur Roux, 75724 Paris cedex 15</addrLine> <country key="FR"></country> </address> <ref type="url">https://research.pasteur.fr/en/team/molecular-genetics-of-rna-viruses/</ref> </desc> <listRelation> <relation name="UMR_3569" active="#struct-441569" type="direct"></relation> <relation name="U-Pasteur_2" active="#struct-300301" type="direct"></relation> <relation active="#struct-300027" type="direct"></relation> </listRelation> <tutelles><tutelle name="UMR_3569" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID"> <idno type="IdRef">02636817X</idno> <idno type="ISNI">0000000122597504</idno> <orgName>Centre National de la Recherche Scientifique</orgName> <orgName type="acronym">CNRS</orgName> <date type="start">1939-10-19</date> <desc> <address> <country key="FR"></country> </address> <ref type="url">http://www.cnrs.fr/</ref> </desc> </org></tutelle><tutelle name="U-Pasteur_2" active="#struct-300301" type="direct"><org type="institution" xml:id="struct-300301" status="OLD"> <idno type="ISNI">0000000121514068</idno> <idno type="IdRef">027542084</idno> <orgName>Université Paris Diderot - Paris 7</orgName> <orgName type="acronym">UPD7</orgName> <date type="end">2019-12-31</date> <desc> <address> <addrLine>5 rue Thomas-Mann - 75205 Paris cedex 13</addrLine> <country key="FR"></country> </address> <ref type="url">http://www.univ-paris-diderot.fr</ref> </desc> </org></tutelle><tutelle active="#struct-300027" type="direct"><org type="institution" xml:id="struct-300027" status="VALID"> <idno type="IdRef">027936643</idno> <idno type="ISNI">0000 0001 2353 6535</idno> <orgName>Institut Pasteur [Paris]</orgName> <date type="start">1887-06-04</date> <desc> <address> <addrLine>25-28, rue du docteur Roux, 75724 Paris cedex 15</addrLine> <country key="FR"></country> </address> <ref type="url">https://www.pasteur.fr</ref> </desc> </org></tutelle></tutelles></hal:affiliation><country>France</country></affiliation></author><author><name sortKey="Roquin, Camille" sort="Roquin, Camille" uniqKey="Roquin C" first="Camille" last="Roquin">Camille Roquin</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-417138" status="OLD"> <idno type="IdRef">235658278</idno> <idno type="RNSR">200818198A</idno> <orgName>Génétique Moléculaire des Virus à ARN - Molecular Genetics of RNA Viruses</orgName> <orgName type="acronym">GMV-ARN (UMR_3569 / U-Pasteur_2)</orgName> <date type="end">2019-12-31</date> <desc> <address> <addrLine>Département de Virologie, 25-28 rue du docteur Roux, 75724 Paris cedex 15</addrLine> <country key="FR"></country> </address> <ref type="url">https://research.pasteur.fr/en/team/molecular-genetics-of-rna-viruses/</ref> </desc> <listRelation> <relation name="UMR_3569" active="#struct-441569" type="direct"></relation> <relation name="U-Pasteur_2" active="#struct-300301" type="direct"></relation> <relation active="#struct-300027" type="direct"></relation> </listRelation> <tutelles><tutelle name="UMR_3569" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID"> <idno type="IdRef">02636817X</idno> <idno type="ISNI">0000000122597504</idno> <orgName>Centre National de la Recherche Scientifique</orgName> <orgName type="acronym">CNRS</orgName> <date type="start">1939-10-19</date> <desc> <address> <country key="FR"></country> </address> <ref type="url">http://www.cnrs.fr/</ref> </desc> </org></tutelle><tutelle name="U-Pasteur_2" active="#struct-300301" type="direct"><org type="institution" xml:id="struct-300301" status="OLD"> <idno type="ISNI">0000000121514068</idno> <idno type="IdRef">027542084</idno> <orgName>Université Paris Diderot - Paris 7</orgName> <orgName type="acronym">UPD7</orgName> <date type="end">2019-12-31</date> <desc> <address> <addrLine>5 rue Thomas-Mann - 75205 Paris cedex 13</addrLine> <country key="FR"></country> </address> <ref type="url">http://www.univ-paris-diderot.fr</ref> </desc> </org></tutelle><tutelle active="#struct-300027" type="direct"><org type="institution" xml:id="struct-300027" status="VALID"> <idno type="IdRef">027936643</idno> <idno type="ISNI">0000 0001 2353 6535</idno> <orgName>Institut Pasteur [Paris]</orgName> <date type="start">1887-06-04</date> <desc> <address> <addrLine>25-28, rue du docteur Roux, 75724 Paris cedex 15</addrLine> <country key="FR"></country> </address> <ref type="url">https://www.pasteur.fr</ref> </desc> </org></tutelle></tutelles></hal:affiliation><country>France</country></affiliation></author><author><name sortKey="Berthault, Camille" sort="Berthault, Camille" uniqKey="Berthault C" first="Camille" last="Berthault">Camille Berthault</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-417138" status="OLD"> <idno type="IdRef">235658278</idno> <idno type="RNSR">200818198A</idno> <orgName>Génétique Moléculaire des Virus à ARN - Molecular Genetics of RNA Viruses</orgName> <orgName type="acronym">GMV-ARN (UMR_3569 / U-Pasteur_2)</orgName> <date type="end">2019-12-31</date> <desc> <address> <addrLine>Département de Virologie, 25-28 rue du docteur Roux, 75724 Paris cedex 15</addrLine> <country key="FR"></country> </address> <ref type="url">https://research.pasteur.fr/en/team/molecular-genetics-of-rna-viruses/</ref> </desc> <listRelation> <relation name="UMR_3569" active="#struct-441569" type="direct"></relation> <relation name="U-Pasteur_2" active="#struct-300301" type="direct"></relation> <relation active="#struct-300027" type="direct"></relation> </listRelation> <tutelles><tutelle name="UMR_3569" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID"> <idno type="IdRef">02636817X</idno> <idno type="ISNI">0000000122597504</idno> <orgName>Centre National de la Recherche Scientifique</orgName> <orgName type="acronym">CNRS</orgName> <date type="start">1939-10-19</date> <desc> <address> <country key="FR"></country> </address> <ref type="url">http://www.cnrs.fr/</ref> </desc> </org></tutelle><tutelle name="U-Pasteur_2" active="#struct-300301" type="direct"><org type="institution" xml:id="struct-300301" status="OLD"> <idno type="ISNI">0000000121514068</idno> <idno type="IdRef">027542084</idno> <orgName>Université Paris Diderot - Paris 7</orgName> <orgName type="acronym">UPD7</orgName> <date type="end">2019-12-31</date> <desc> <address> <addrLine>5 rue Thomas-Mann - 75205 Paris cedex 13</addrLine> <country key="FR"></country> </address> <ref type="url">http://www.univ-paris-diderot.fr</ref> </desc> </org></tutelle><tutelle active="#struct-300027" type="direct"><org type="institution" xml:id="struct-300027" status="VALID"> <idno type="IdRef">027936643</idno> <idno type="ISNI">0000 0001 2353 6535</idno> <orgName>Institut Pasteur [Paris]</orgName> <date type="start">1887-06-04</date> <desc> <address> <addrLine>25-28, rue du docteur Roux, 75724 Paris cedex 15</addrLine> <country key="FR"></country> </address> <ref type="url">https://www.pasteur.fr</ref> </desc> </org></tutelle></tutelles></hal:affiliation><country>France</country></affiliation></author><author><name sortKey="Van Der Werf, Sylvie" sort="Van Der Werf, Sylvie" uniqKey="Van Der Werf S" first="Sylvie" last="Van Der Werf">Sylvie Van Der Werf</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-417138" status="OLD"> <idno type="IdRef">235658278</idno> <idno type="RNSR">200818198A</idno> <orgName>Génétique Moléculaire des Virus à ARN - Molecular Genetics of RNA Viruses</orgName> <orgName type="acronym">GMV-ARN (UMR_3569 / U-Pasteur_2)</orgName> <date type="end">2019-12-31</date> <desc> <address> <addrLine>Département de Virologie, 25-28 rue du docteur Roux, 75724 Paris cedex 15</addrLine> <country key="FR"></country> </address> <ref type="url">https://research.pasteur.fr/en/team/molecular-genetics-of-rna-viruses/</ref> </desc> <listRelation> <relation name="UMR_3569" active="#struct-441569" type="direct"></relation> <relation name="U-Pasteur_2" active="#struct-300301" type="direct"></relation> <relation active="#struct-300027" type="direct"></relation> </listRelation> <tutelles><tutelle name="UMR_3569" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID"> <idno type="IdRef">02636817X</idno> <idno type="ISNI">0000000122597504</idno> <orgName>Centre National de la Recherche Scientifique</orgName> <orgName type="acronym">CNRS</orgName> <date type="start">1939-10-19</date> <desc> <address> <country key="FR"></country> </address> <ref type="url">http://www.cnrs.fr/</ref> </desc> </org></tutelle><tutelle name="U-Pasteur_2" active="#struct-300301" type="direct"><org type="institution" xml:id="struct-300301" status="OLD"> <idno type="ISNI">0000000121514068</idno> <idno type="IdRef">027542084</idno> <orgName>Université Paris Diderot - Paris 7</orgName> <orgName type="acronym">UPD7</orgName> <date type="end">2019-12-31</date> <desc> <address> <addrLine>5 rue Thomas-Mann - 75205 Paris cedex 13</addrLine> <country key="FR"></country> </address> <ref type="url">http://www.univ-paris-diderot.fr</ref> </desc> </org></tutelle><tutelle active="#struct-300027" type="direct"><org type="institution" xml:id="struct-300027" status="VALID"> <idno type="IdRef">027936643</idno> <idno type="ISNI">0000 0001 2353 6535</idno> <orgName>Institut Pasteur [Paris]</orgName> <date type="start">1887-06-04</date> <desc> <address> <addrLine>25-28, rue du docteur Roux, 75724 Paris cedex 15</addrLine> <country key="FR"></country> </address> <ref type="url">https://www.pasteur.fr</ref> </desc> </org></tutelle></tutelles></hal:affiliation><country>France</country></affiliation></author></analytic><idno type="DOI">10.1038/srep43462</idno><series><title level="j">Scientific Reports</title><idno type="ISSN">2045-2322</idno><imprint><date type="datePub">2017-02-27</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="mix" xml:lang="en"><term>Influenza virus</term><term>Non-coding RNAs</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"> <p>For each influenza virus genome segment, the coding sequence is flanked by non-coding (NC) regions comprising shared, conserved sequences and specific, non-conserved sequences. The latter and adjacent parts of the coding sequence are involved in genome packaging, but the precise role of the non-conserved NC sequences is still unclear. The aim of this study is to better understand the role of the non-conserved non-coding sequences in the incorporation of the viral segments into virions. The NA-segment NC sequences were systematically replaced by those of the seven other segments. Recombinant viruses harbouring two segments with identical NC sequences were successfully rescued. Virus growth kinetics and serial passages were performed, and incorporation of the viral segments was tested by real-time RT-PCR. An initial virus growth deficiency correlated to a specific defect in NA segment incorporation. Upon serial passages, growth properties were restored. Sequencing revealed that the replacing 5'NC sequence length drove the type of mutations obtained. With sequences longer than the original, point mutations in the coding region with or without substitutions in the 3'NC region were detected. With shorter sequences, insertions were observed in the 5'NC region. Restoration of viral fitness was linked to restoration of the NA segment incorporation.</p> </div></front></TEI><hal api="V3"> <titleStmt> <title xml:lang="eng">Incorporation of the influenza A virus NA segment into virions does not require cognate non-coding sequences.</title> <author role="aut"> <persName> <forename type="first">Bernadette</forename> <surname>Crescenzo-Chaigne</surname> </persName> <idno type="halauthorid">203159</idno> <affiliation ref="#struct-417138"></affiliation> </author> <author role="aut"> <persName> <forename type="first">Cyril V S</forename> <surname>Barbezange</surname> </persName> <idno type="halauthorid">1595351</idno> <affiliation ref="#struct-417138"></affiliation> </author> <author role="aut"> <persName> <forename type="first">Stéphane</forename> <surname>Léandri</surname> </persName> <idno type="halauthorid">1595352</idno> <affiliation ref="#struct-417138"></affiliation> </author> <author role="aut"> <persName> <forename type="first">Camille</forename> <surname>Roquin</surname> </persName> <idno type="halauthorid">1595353</idno> <affiliation ref="#struct-417138"></affiliation> </author> <author role="aut"> <persName> <forename type="first">Camille</forename> <surname>Berthault</surname> </persName> <email type="md5">ccbdb933c469a6ec7133521fd6f30c62</email> <email type="domain">inra.fr</email> <idno type="halauthorid">1595354</idno> <affiliation ref="#struct-417138"></affiliation> </author> <author role="crp"> <persName> <forename type="first">Sylvie</forename> <surname>Van Der Werf</surname> </persName> <email type="md5">548b93020fd78f6b62853b198dd267d4</email> <email type="domain">pasteur.fr</email> <idno type="halauthorid">632819</idno> <affiliation ref="#struct-417138"></affiliation> </author> <editor role="depositor"> <persName> <forename>Floran</forename> <surname>Bidault</surname> </persName> <email type="md5">49ef5398ff356d5e1f225eb0aa0c4b3e</email> <email type="domain">pasteur.fr</email> </editor> <funder ref="#projanr-37477"></funder> <funder ref="#projeurop-89460"></funder> <funder>This work was supported by funds from the ARN grant Labex ‘Integrative Biology of Emerging Infectious Diseases’ (grant number ANR-10-LABX-62-IBEID) from the French Government’s ‘Investissement d’Avenir’ programme, and from the PREDEMICS consortium (grant agreement number 278433) from the European Community Seventh Framework Programme FP7/2007–2013. </funder> </titleStmt> <editionStmt> <edition n="v1" type="current"> <date type="whenSubmitted">2017-07-28 16:20:27</date> <date type="whenModified">2020-05-26 10:46:02</date> <date type="whenReleased">2017-08-03 12:48:18</date> <date type="whenProduced">2017-02-27</date> <date type="whenEndEmbargoed">2017-08-03</date> <ref type="file" target="https://hal-pasteur.archives-ouvertes.fr/pasteur-01570194/document"> <date notBefore="2017-08-03"></date> </ref> <ref type="file" subtype="publisherPaid" n="1" target="https://hal-pasteur.archives-ouvertes.fr/pasteur-01570194/file/srep43462.pdf"> <date notBefore="2017-08-03"></date> </ref> <ref type="externalLink" target="https://www.nature.com/articles/srep43462.pdf"></ref> </edition> <respStmt> <resp>contributor</resp> <name key="328609"> <persName> <forename>Floran</forename> <surname>Bidault</surname> </persName> <email type="md5">49ef5398ff356d5e1f225eb0aa0c4b3e</email> <email type="domain">pasteur.fr</email> </name> </respStmt> </editionStmt> <publicationStmt> <distributor>CCSD</distributor> <idno type="halId">pasteur-01570194</idno> <idno type="halUri">https://hal-pasteur.archives-ouvertes.fr/pasteur-01570194</idno> <idno type="halBibtex">crescenzochaigne:pasteur-01570194</idno> <idno type="halRefHtml">Scientific Reports, Nature Publishing Group, 2017, 7, pp.43462. ⟨10.1038/srep43462⟩</idno> <idno type="halRef">Scientific Reports, Nature Publishing Group, 2017, 7, pp.43462. ⟨10.1038/srep43462⟩</idno> <availability status="restricted"> <licence target="http://creativecommons.org/licenses/by/">Attribution</licence> </availability> </publicationStmt> <seriesStmt> <idno type="stamp" n="PASTEUR">Institut Pasteur</idno> <idno type="stamp" n="CNRS">CNRS - Centre national de la recherche scientifique</idno> <idno type="stamp" n="OPENAIRE">OpenAIRE</idno> <idno type="stamp" n="UNIV-PARIS7" corresp="UNIV-PARIS">Université Denis Diderot - Paris VII</idno> <idno type="stamp" n="USPC">Université Sorbonne Paris Cité</idno> <idno type="stamp" n="UNIV-PARIS">Université de Paris</idno> <idno type="stamp" n="UP-SCIENCES">Université de Paris - Faculté des Sciences</idno> </seriesStmt> <notesStmt> <note type="audience" n="2">International</note> <note type="popular" n="0">No</note> <note type="peer" n="1">Yes</note> </notesStmt> <sourceDesc> <biblStruct> <analytic> <title xml:lang="eng">Incorporation of the influenza A virus NA segment into virions does not require cognate non-coding sequences.</title> <author role="aut"> <persName> <forename type="first">Bernadette</forename> <surname>Crescenzo-Chaigne</surname> </persName> <idno type="halauthorid">203159</idno> <affiliation ref="#struct-417138"></affiliation> </author> <author role="aut"> <persName> <forename type="first">Cyril V S</forename> <surname>Barbezange</surname> </persName> <idno type="halauthorid">1595351</idno> <affiliation ref="#struct-417138"></affiliation> </author> <author role="aut"> <persName> <forename type="first">Stéphane</forename> <surname>Léandri</surname> </persName> <idno type="halauthorid">1595352</idno> <affiliation ref="#struct-417138"></affiliation> </author> <author role="aut"> <persName> <forename type="first">Camille</forename> <surname>Roquin</surname> </persName> <idno type="halauthorid">1595353</idno> <affiliation ref="#struct-417138"></affiliation> </author> <author role="aut"> <persName> <forename type="first">Camille</forename> <surname>Berthault</surname> </persName> <email type="md5">ccbdb933c469a6ec7133521fd6f30c62</email> <email type="domain">inra.fr</email> <idno type="halauthorid">1595354</idno> <affiliation ref="#struct-417138"></affiliation> </author> <author role="crp"> <persName> <forename type="first">Sylvie</forename> <surname>Van Der Werf</surname> </persName> <email type="md5">548b93020fd78f6b62853b198dd267d4</email> <email type="domain">pasteur.fr</email> <idno type="halauthorid">632819</idno> <affiliation ref="#struct-417138"></affiliation> </author> </analytic> <monogr> <idno type="halJournalId" status="VALID">89181</idno> <idno type="issn">2045-2322</idno> <idno type="eissn">2045-2322</idno> <title level="j">Scientific Reports</title> <imprint> <publisher>Nature Publishing Group</publisher> <biblScope unit="volume">7</biblScope> <biblScope unit="pp">43462</biblScope> <date type="datePub">2017-02-27</date> </imprint> </monogr> <idno type="doi">10.1038/srep43462</idno> <idno type="pubmed">28240311</idno> <ref type="seeAlso" target="https://images.nature.com/original/nature-assets/srep/2017/170227/srep43462/extref/srep43462-s1.pdf">https://images.nature.com/original/nature-assets/srep/2017/170227/srep43462/extref/srep43462-s1.pdf</ref> </biblStruct> </sourceDesc> <profileDesc> <langUsage> <language ident="en">English</language> </langUsage> <textClass> <keywords scheme="author"> <term xml:lang="en">Influenza virus</term> <term xml:lang="en">Non-coding RNAs</term> </keywords> <classCode scheme="halDomain" n="sdv">Life Sciences [q-bio]</classCode> <classCode scheme="halDomain" n="sdv.mp.vir">Life Sciences [q-bio]/Microbiology and Parasitology/Virology</classCode> <classCode scheme="halTypology" n="ART">Journal articles</classCode> </textClass> <abstract xml:lang="en"> <p>For each influenza virus genome segment, the coding sequence is flanked by non-coding (NC) regions comprising shared, conserved sequences and specific, non-conserved sequences. The latter and adjacent parts of the coding sequence are involved in genome packaging, but the precise role of the non-conserved NC sequences is still unclear. The aim of this study is to better understand the role of the non-conserved non-coding sequences in the incorporation of the viral segments into virions. The NA-segment NC sequences were systematically replaced by those of the seven other segments. Recombinant viruses harbouring two segments with identical NC sequences were successfully rescued. Virus growth kinetics and serial passages were performed, and incorporation of the viral segments was tested by real-time RT-PCR. An initial virus growth deficiency correlated to a specific defect in NA segment incorporation. Upon serial passages, growth properties were restored. Sequencing revealed that the replacing 5'NC sequence length drove the type of mutations obtained. With sequences longer than the original, point mutations in the coding region with or without substitutions in the 3'NC region were detected. With shorter sequences, insertions were observed in the 5'NC region. Restoration of viral fitness was linked to restoration of the NA segment incorporation.</p> </abstract> <particDesc> <org type="consortium">The authors are very grateful to G. Brownlee for providing the pPOLI expression plasmids of influenza virus A/WSN/33 and to J. Pavlovic for the four pHMG recombinant plasmids of influenza virus A/PR/8/34, and wish to thank C. Isel-Griffiths, S. Munier and M. Declercq for helpful discussions and critical reading of the manuscript.</org> </particDesc> </profileDesc> </hal></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/PandemieGrippaleV1/Data/Hal/Checkpoint
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000276 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Hal/Checkpoint/biblio.hfd -nk 000276 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= PandemieGrippaleV1
|flux= Hal
|étape= Checkpoint
|type= RBID
|clé= Hal:pasteur-01570194
|texte= Incorporation of the influenza A virus NA segment into virions does not require cognate non-coding sequences.
}}
| This area was generated with Dilib version V0.6.34. |  |