Ancient mitochondrial DNA analyses of ascaris eggs discovered in coprolites from joseon tomb.
Identifieur interne : 000112 ( PubMed/Curation ); précédent : 000111; suivant : 000113Ancient mitochondrial DNA analyses of ascaris eggs discovered in coprolites from joseon tomb.
Auteurs : Chang Seok Oh [Corée du Sud] ; Min Seo [Corée du Sud] ; Jong Ha Hong [Corée du Sud] ; Jong-Yil Chai [Corée du Sud] ; Seung Whan Oh [Corée du Sud] ; Jun Bum Park [Corée du Sud] ; Dong Hoon Shin [Corée du Sud]Source :
- The Korean journal of parasitology [ 1738-0006 ] ; 2015.
English descriptors
- KwdEn :
- Adult, Animals, Ascariasis (diagnosis), Ascariasis (history), Ascariasis (parasitology), Ascaris (classification), Ascaris (genetics), Ascaris (isolation & purification), Base Sequence, Cytochromes b (genetics), DNA Primers (genetics), DNA, Helminth (genetics), DNA, Mitochondrial (genetics), DNA, Mitochondrial (history), Female, Fossils (history), Fossils (parasitology), History, Ancient, Humans, Male, Molecular Sequence Data, Mummies (history), Mummies (parasitology), Ovum (chemistry), Ovum (classification), Phylogeny, RNA, Ribosomal, 18S (genetics).
- MESH :
- chemical , genetics : Cytochromes b, DNA Primers, DNA, Helminth, DNA, Mitochondrial, RNA, Ribosomal, 18S.
- chemistry : Ovum.
- classification : Ascaris, Ovum.
- diagnosis : Ascariasis.
- genetics : Ascaris.
- history : Ascariasis, DNA, Mitochondrial, Fossils, Mummies.
- isolation & purification : Ascaris.
- parasitology : Ascariasis, Fossils, Mummies.
- Adult, Animals, Base Sequence, Female, History, Ancient, Humans, Male, Molecular Sequence Data, Phylogeny.
Abstract
Analysis of ancient DNA (aDNA) extracted from Ascaris is very important for understanding the phylogenetic lineage of the parasite species. When aDNAs obtained from a Joseon tomb (SN2-19-1) coprolite in which Ascaris eggs were identified were amplified with primers for cytochrome b (cyt b) and 18S small subunit ribosomal RNA (18S rRNA) gene, the outcome exhibited Ascaris specific amplicon bands. By cloning, sequencing, and analysis of the amplified DNA, we obtained information valuable for comprehending genetic lineage of Ascaris prevalent among pre-modern Joseon peoples.
DOI: 10.3347/kjp.2015.53.2.237
PubMed: 25925186
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<front><div type="abstract" xml:lang="en">Analysis of ancient DNA (aDNA) extracted from Ascaris is very important for understanding the phylogenetic lineage of the parasite species. When aDNAs obtained from a Joseon tomb (SN2-19-1) coprolite in which Ascaris eggs were identified were amplified with primers for cytochrome b (cyt b) and 18S small subunit ribosomal RNA (18S rRNA) gene, the outcome exhibited Ascaris specific amplicon bands. By cloning, sequencing, and analysis of the amplified DNA, we obtained information valuable for comprehending genetic lineage of Ascaris prevalent among pre-modern Joseon peoples.</div>
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<Abstract><AbstractText>Analysis of ancient DNA (aDNA) extracted from Ascaris is very important for understanding the phylogenetic lineage of the parasite species. When aDNAs obtained from a Joseon tomb (SN2-19-1) coprolite in which Ascaris eggs were identified were amplified with primers for cytochrome b (cyt b) and 18S small subunit ribosomal RNA (18S rRNA) gene, the outcome exhibited Ascaris specific amplicon bands. By cloning, sequencing, and analysis of the amplified DNA, we obtained information valuable for comprehending genetic lineage of Ascaris prevalent among pre-modern Joseon peoples.</AbstractText>
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<Day>11</Day>
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<PubMedPubDate PubStatus="revised"><Year>2015</Year>
<Month>02</Month>
<Day>25</Day>
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<PubMedPubDate PubStatus="accepted"><Year>2015</Year>
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<ArticleIdList><ArticleId IdType="pubmed">25925186</ArticleId>
<ArticleId IdType="doi">10.3347/kjp.2015.53.2.237</ArticleId>
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