Validation of qPCR Methods for the Detection of Mycobacterium in New World Animal Reservoirs
Identifieur interne : 000029 ( Pmc/Checkpoint ); précédent : 000028; suivant : 000030Validation of qPCR Methods for the Detection of Mycobacterium in New World Animal Reservoirs
Auteurs : Genevieve Housman [États-Unis] ; Joanna Malukiewicz [États-Unis, Brésil] ; Vanner Boere [Brésil] ; Adriana D. Grativol [Brésil] ; Luiz Cezar M. Pereira [Brésil] ; Ita De Oliveira E Silva [Brésil] ; Carlos R. Ruiz-Miranda [Brésil] ; Richard Truman [États-Unis] ; Anne C. Stone [États-Unis]Source :
- PLoS Neglected Tropical Diseases [ 1935-2727 ] ; 2015.
Abstract
Zoonotic pathogens that cause leprosy (
Url:
DOI: 10.1371/journal.pntd.0004198
PubMed: 26571269
PubMed Central: 4646627
Affiliations:
- Brésil, États-Unis
- Arizona, Louisiane, Minas Gerais, Pernambuco, État de Rio de Janeiro
- Rio de Janeiro
Links toward previous steps (curation, corpus...)
Links to Exploration step
PMC:4646627Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Validation of qPCR Methods for the Detection of <italic>Mycobacterium</italic> in New World Animal Reservoirs</title><author><name sortKey="Housman, Genevieve" sort="Housman, Genevieve" uniqKey="Housman G" first="Genevieve" last="Housman">Genevieve Housman</name><affiliation wicri:level="2"><nlm:aff id="aff001"><addr-line>School of Human Evolution and Social Change, Arizona State University, Tempe, Arizona, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>School of Human Evolution and Social Change, Arizona State University, Tempe, Arizona</wicri:regionArea><placeName><region type="state">Arizona</region></placeName></affiliation></author><author><name sortKey="Malukiewicz, Joanna" sort="Malukiewicz, Joanna" uniqKey="Malukiewicz J" first="Joanna" last="Malukiewicz">Joanna Malukiewicz</name><affiliation wicri:level="2"><nlm:aff id="aff001"><addr-line>School of Human Evolution and Social Change, Arizona State University, Tempe, Arizona, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>School of Human Evolution and Social Change, Arizona State University, Tempe, Arizona</wicri:regionArea><placeName><region type="state">Arizona</region></placeName></affiliation><affiliation wicri:level="2"><nlm:aff id="aff002"><addr-line>Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brazil</addr-line></nlm:aff><country xml:lang="fr">Brésil</country><wicri:regionArea>Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Viçosa, Viçosa, Minas Gerais</wicri:regionArea><placeName><region type="state">Minas Gerais</region></placeName></affiliation></author><author><name sortKey="Boere, Vanner" sort="Boere, Vanner" uniqKey="Boere V" first="Vanner" last="Boere">Vanner Boere</name><affiliation wicri:level="2"><nlm:aff id="aff002"><addr-line>Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brazil</addr-line></nlm:aff><country xml:lang="fr">Brésil</country><wicri:regionArea>Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Viçosa, Viçosa, Minas Gerais</wicri:regionArea><placeName><region type="state">Minas Gerais</region></placeName></affiliation></author><author><name sortKey="Grativol, Adriana D" sort="Grativol, Adriana D" uniqKey="Grativol A" first="Adriana D." last="Grativol">Adriana D. Grativol</name><affiliation wicri:level="3"><nlm:aff id="aff003"><addr-line>Laboratório de Ciências Ambientias, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense, Campos dos Goytacazes, Rio de Janeiro, Brazil</addr-line></nlm:aff><country xml:lang="fr">Brésil</country><wicri:regionArea>Laboratório de Ciências Ambientias, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense, Campos dos Goytacazes, Rio de Janeiro</wicri:regionArea><placeName><settlement type="city">Rio de Janeiro</settlement><region type="state">État de Rio de Janeiro</region></placeName></affiliation></author><author><name sortKey="Pereira, Luiz Cezar M" sort="Pereira, Luiz Cezar M" uniqKey="Pereira L" first="Luiz Cezar M." last="Pereira">Luiz Cezar M. Pereira</name><affiliation wicri:level="2"><nlm:aff id="aff004"><addr-line>Centro de Conservação e Manejo de Fauna da Caatinga, Universidade Federal do Vale do São Francisco, Petrolina, Pernambuco, Brazil</addr-line></nlm:aff><country xml:lang="fr">Brésil</country><wicri:regionArea>Centro de Conservação e Manejo de Fauna da Caatinga, Universidade Federal do Vale do São Francisco, Petrolina, Pernambuco</wicri:regionArea><placeName><region type="state">Pernambuco</region></placeName></affiliation></author><author><name sortKey="Silva, Ita De Oliveira E" sort="Silva, Ita De Oliveira E" uniqKey="Silva I" first="Ita De Oliveira E" last="Silva">Ita De Oliveira E Silva</name><affiliation wicri:level="2"><nlm:aff id="aff005"><addr-line>Departamento de Biologia Animal, Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brazil</addr-line></nlm:aff><country xml:lang="fr">Brésil</country><wicri:regionArea>Departamento de Biologia Animal, Universidade Federal de Viçosa, Viçosa, Minas Gerais</wicri:regionArea><placeName><region type="state">Minas Gerais</region></placeName></affiliation></author><author><name sortKey="Ruiz Miranda, Carlos R" sort="Ruiz Miranda, Carlos R" uniqKey="Ruiz Miranda C" first="Carlos R." last="Ruiz-Miranda">Carlos R. Ruiz-Miranda</name><affiliation wicri:level="3"><nlm:aff id="aff003"><addr-line>Laboratório de Ciências Ambientias, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense, Campos dos Goytacazes, Rio de Janeiro, Brazil</addr-line></nlm:aff><country xml:lang="fr">Brésil</country><wicri:regionArea>Laboratório de Ciências Ambientias, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense, Campos dos Goytacazes, Rio de Janeiro</wicri:regionArea><placeName><settlement type="city">Rio de Janeiro</settlement><region type="state">État de Rio de Janeiro</region></placeName></affiliation></author><author><name sortKey="Truman, Richard" sort="Truman, Richard" uniqKey="Truman R" first="Richard" last="Truman">Richard Truman</name><affiliation wicri:level="2"><nlm:aff id="aff006"><addr-line>HHS\HRSA\HSB National Hansen's Disease Program-NIAID IAA-2646, Baton Rouge, Louisiana, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>HHS\HRSA\HSB National Hansen's Disease Program-NIAID IAA-2646, Baton Rouge, Louisiana</wicri:regionArea><placeName><region type="state">Louisiane</region></placeName></affiliation></author><author><name sortKey="Stone, Anne C" sort="Stone, Anne C" uniqKey="Stone A" first="Anne C." last="Stone">Anne C. Stone</name><affiliation wicri:level="2"><nlm:aff id="aff001"><addr-line>School of Human Evolution and Social Change, Arizona State University, Tempe, Arizona, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>School of Human Evolution and Social Change, Arizona State University, Tempe, Arizona</wicri:regionArea><placeName><region type="state">Arizona</region></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">26571269</idno><idno type="pmc">4646627</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4646627</idno><idno type="RBID">PMC:4646627</idno><idno type="doi">10.1371/journal.pntd.0004198</idno><date when="2015">2015</date><idno type="wicri:Area/Pmc/Corpus">000017</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000017</idno><idno type="wicri:Area/Pmc/Curation">000017</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000017</idno><idno type="wicri:Area/Pmc/Checkpoint">000029</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000029</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Validation of qPCR Methods for the Detection of <italic>Mycobacterium</italic> in New World Animal Reservoirs</title><author><name sortKey="Housman, Genevieve" sort="Housman, Genevieve" uniqKey="Housman G" first="Genevieve" last="Housman">Genevieve Housman</name><affiliation wicri:level="2"><nlm:aff id="aff001"><addr-line>School of Human Evolution and Social Change, Arizona State University, Tempe, Arizona, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>School of Human Evolution and Social Change, Arizona State University, Tempe, Arizona</wicri:regionArea><placeName><region type="state">Arizona</region></placeName></affiliation></author><author><name sortKey="Malukiewicz, Joanna" sort="Malukiewicz, Joanna" uniqKey="Malukiewicz J" first="Joanna" last="Malukiewicz">Joanna Malukiewicz</name><affiliation wicri:level="2"><nlm:aff id="aff001"><addr-line>School of Human Evolution and Social Change, Arizona State University, Tempe, Arizona, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>School of Human Evolution and Social Change, Arizona State University, Tempe, Arizona</wicri:regionArea><placeName><region type="state">Arizona</region></placeName></affiliation><affiliation wicri:level="2"><nlm:aff id="aff002"><addr-line>Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brazil</addr-line></nlm:aff><country xml:lang="fr">Brésil</country><wicri:regionArea>Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Viçosa, Viçosa, Minas Gerais</wicri:regionArea><placeName><region type="state">Minas Gerais</region></placeName></affiliation></author><author><name sortKey="Boere, Vanner" sort="Boere, Vanner" uniqKey="Boere V" first="Vanner" last="Boere">Vanner Boere</name><affiliation wicri:level="2"><nlm:aff id="aff002"><addr-line>Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brazil</addr-line></nlm:aff><country xml:lang="fr">Brésil</country><wicri:regionArea>Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Viçosa, Viçosa, Minas Gerais</wicri:regionArea><placeName><region type="state">Minas Gerais</region></placeName></affiliation></author><author><name sortKey="Grativol, Adriana D" sort="Grativol, Adriana D" uniqKey="Grativol A" first="Adriana D." last="Grativol">Adriana D. Grativol</name><affiliation wicri:level="3"><nlm:aff id="aff003"><addr-line>Laboratório de Ciências Ambientias, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense, Campos dos Goytacazes, Rio de Janeiro, Brazil</addr-line></nlm:aff><country xml:lang="fr">Brésil</country><wicri:regionArea>Laboratório de Ciências Ambientias, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense, Campos dos Goytacazes, Rio de Janeiro</wicri:regionArea><placeName><settlement type="city">Rio de Janeiro</settlement><region type="state">État de Rio de Janeiro</region></placeName></affiliation></author><author><name sortKey="Pereira, Luiz Cezar M" sort="Pereira, Luiz Cezar M" uniqKey="Pereira L" first="Luiz Cezar M." last="Pereira">Luiz Cezar M. Pereira</name><affiliation wicri:level="2"><nlm:aff id="aff004"><addr-line>Centro de Conservação e Manejo de Fauna da Caatinga, Universidade Federal do Vale do São Francisco, Petrolina, Pernambuco, Brazil</addr-line></nlm:aff><country xml:lang="fr">Brésil</country><wicri:regionArea>Centro de Conservação e Manejo de Fauna da Caatinga, Universidade Federal do Vale do São Francisco, Petrolina, Pernambuco</wicri:regionArea><placeName><region type="state">Pernambuco</region></placeName></affiliation></author><author><name sortKey="Silva, Ita De Oliveira E" sort="Silva, Ita De Oliveira E" uniqKey="Silva I" first="Ita De Oliveira E" last="Silva">Ita De Oliveira E Silva</name><affiliation wicri:level="2"><nlm:aff id="aff005"><addr-line>Departamento de Biologia Animal, Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brazil</addr-line></nlm:aff><country xml:lang="fr">Brésil</country><wicri:regionArea>Departamento de Biologia Animal, Universidade Federal de Viçosa, Viçosa, Minas Gerais</wicri:regionArea><placeName><region type="state">Minas Gerais</region></placeName></affiliation></author><author><name sortKey="Ruiz Miranda, Carlos R" sort="Ruiz Miranda, Carlos R" uniqKey="Ruiz Miranda C" first="Carlos R." last="Ruiz-Miranda">Carlos R. Ruiz-Miranda</name><affiliation wicri:level="3"><nlm:aff id="aff003"><addr-line>Laboratório de Ciências Ambientias, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense, Campos dos Goytacazes, Rio de Janeiro, Brazil</addr-line></nlm:aff><country xml:lang="fr">Brésil</country><wicri:regionArea>Laboratório de Ciências Ambientias, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense, Campos dos Goytacazes, Rio de Janeiro</wicri:regionArea><placeName><settlement type="city">Rio de Janeiro</settlement><region type="state">État de Rio de Janeiro</region></placeName></affiliation></author><author><name sortKey="Truman, Richard" sort="Truman, Richard" uniqKey="Truman R" first="Richard" last="Truman">Richard Truman</name><affiliation wicri:level="2"><nlm:aff id="aff006"><addr-line>HHS\HRSA\HSB National Hansen's Disease Program-NIAID IAA-2646, Baton Rouge, Louisiana, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>HHS\HRSA\HSB National Hansen's Disease Program-NIAID IAA-2646, Baton Rouge, Louisiana</wicri:regionArea><placeName><region type="state">Louisiane</region></placeName></affiliation></author><author><name sortKey="Stone, Anne C" sort="Stone, Anne C" uniqKey="Stone A" first="Anne C." last="Stone">Anne C. Stone</name><affiliation wicri:level="2"><nlm:aff id="aff001"><addr-line>School of Human Evolution and Social Change, Arizona State University, Tempe, Arizona, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>School of Human Evolution and Social Change, Arizona State University, Tempe, Arizona</wicri:regionArea><placeName><region type="state">Arizona</region></placeName></affiliation></author></analytic><series><title level="j">PLoS Neglected Tropical Diseases</title><idno type="ISSN">1935-2727</idno><idno type="eISSN">1935-2735</idno><imprint><date when="2015">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Zoonotic pathogens that cause leprosy (<italic>Mycobacterium leprae</italic>) and tuberculosis (<italic>Mycobacterium tuberculosis</italic> complex, MTBC) continue to impact modern human populations. Therefore, methods able to survey mycobacterial infection in potential animal hosts are necessary for proper evaluation of human exposure threats. Here we tested for mycobacterial-specific single- and multi-copy loci using qPCR. In a trial study in which armadillos were artificially infected with <italic>M</italic>. <italic>leprae</italic>, these techniques were specific and sensitive to pathogen detection, while more traditional ELISAs were only specific. These assays were then employed in a case study to detect <italic>M</italic>. <italic>leprae</italic> as well as MTBC in wild marmosets. All marmosets were negative for <italic>M</italic>. <italic>leprae</italic> DNA, but 14 were positive for the mycobacterial rpoB gene assay. Targeted capture and sequencing of rpoB and other MTBC genes validated the presence of mycobacterial DNA in these samples and revealed that qPCR is useful for identifying mycobacterial-infected animal hosts.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Greger, M" uniqKey="Greger M">M Greger</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wolfe, Nd" uniqKey="Wolfe N">ND Wolfe</name></author><author><name sortKey="Dunavan, Cp" uniqKey="Dunavan C">CP Dunavan</name></author><author><name sortKey="Diamond, J" uniqKey="Diamond J">J Diamond</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zakham, F" uniqKey="Zakham F">F Zakham</name></author><author><name sortKey="Aouane, O" uniqKey="Aouane O">O Aouane</name></author><author><name sortKey="Ussery, D" uniqKey="Ussery D">D Ussery</name></author><author><name sortKey="Benjouad, A" uniqKey="Benjouad A">A Benjouad</name></author><author><name sortKey="Ennaji, Mm" uniqKey="Ennaji M">MM Ennaji</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Klaus, Hd" uniqKey="Klaus H">HD Klaus</name></author><author><name sortKey="Wilbur, Ak" uniqKey="Wilbur A">AK Wilbur</name></author><author><name sortKey="Temple, Dh" uniqKey="Temple D">DH Temple</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wilbur, Ak" uniqKey="Wilbur A">AK Wilbur</name></author><author><name sortKey="Engel, Ga" uniqKey="Engel G">GA Engel</name></author><author><name sortKey="Rompis, A" uniqKey="Rompis A">A Rompis</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Montali, Rj" uniqKey="Montali R">RJ Montali</name></author><author><name sortKey="Mikota, Sk" uniqKey="Mikota S">SK Mikota</name></author><author><name sortKey="Cheng, Li" uniqKey="Cheng L">LI Cheng</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Brusasca, Pn" uniqKey="Brusasca P">PN Brusasca</name></author><author><name sortKey="Peters, Rl" uniqKey="Peters R">RL Peters</name></author><author><name sortKey="Motzel, Sl" uniqKey="Motzel S">SL Motzel</name></author><author><name sortKey="Klein, Hj" uniqKey="Klein H">HJ Klein</name></author><author><name sortKey="Gennaro, Ml" uniqKey="Gennaro M">ML Gennaro</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Griffith, S" uniqKey="Griffith S">S Griffith</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Truman, Rw" uniqKey="Truman R">RW Truman</name></author><author><name sortKey="Singh, P" uniqKey="Singh P">P Singh</name></author><author><name sortKey="Sharma, R" uniqKey="Sharma R">R Sharma</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Donham, Kj" uniqKey="Donham K">KJ Donham</name></author><author><name sortKey="Leininger, Jr" uniqKey="Leininger J">JR Leininger</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Valverde, Cr" uniqKey="Valverde C">CR Valverde</name></author><author><name sortKey="Canfield, D" uniqKey="Canfield D">D Canfield</name></author><author><name sortKey="Tarara, R" uniqKey="Tarara R">R Tarara</name></author><author><name sortKey="Esteves, Mi" uniqKey="Esteves M">MI Esteves</name></author><author><name sortKey="Gormus, Bj" uniqKey="Gormus B">BJ Gormus</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Meyers, Wm" uniqKey="Meyers W">WM Meyers</name></author><author><name sortKey="Gormus, Bj" uniqKey="Gormus B">BJ Gormus</name></author><author><name sortKey="Walsh, Gp" uniqKey="Walsh G">GP Walsh</name></author><author><name sortKey="Baskin, Gb" uniqKey="Baskin G">GB Baskin</name></author><author><name sortKey="Hubbard, Gb" uniqKey="Hubbard G">GB Hubbard</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Traub, Rj" uniqKey="Traub R">RJ Traub</name></author><author><name sortKey="Monis, Pt" uniqKey="Monis P">PT Monis</name></author><author><name sortKey="Robertson, Id" uniqKey="Robertson I">ID Robertson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Miller, M" uniqKey="Miller M">M Miller</name></author><author><name sortKey="Terrell, S" uniqKey="Terrell S">S Terrell</name></author><author><name sortKey="Lyashchenko, K" uniqKey="Lyashchenko K">K Lyashchenko</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Truman, Rw" uniqKey="Truman R">RW Truman</name></author><author><name sortKey="Andrews, Pk" uniqKey="Andrews P">PK Andrews</name></author><author><name sortKey="Robbins, Ny" uniqKey="Robbins N">NY Robbins</name></author><author><name sortKey="Adams, Lb" uniqKey="Adams L">LB Adams</name></author><author><name sortKey="Krahenbuhl, Jl" uniqKey="Krahenbuhl J">JL Krahenbuhl</name></author><author><name sortKey="Gillis, Tp" uniqKey="Gillis T">TP Gillis</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Martinez, An" uniqKey="Martinez A">AN Martinez</name></author><author><name sortKey="Britto, Cfpc" uniqKey="Britto C">CFPC Britto</name></author><author><name sortKey="Nery, Jac" uniqKey="Nery J">JAC Nery</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Harkins, Km" uniqKey="Harkins K">KM Harkins</name></author><author><name sortKey="Buikstra, Je" uniqKey="Buikstra J">JE Buikstra</name></author><author><name sortKey="Campbell, T" uniqKey="Campbell T">T Campbell</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Thorne, N" uniqKey="Thorne N">N Thorne</name></author><author><name sortKey="Borrell, S" uniqKey="Borrell S">S Borrell</name></author><author><name sortKey="Evans, J" uniqKey="Evans J">J Evans</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mendes Pontes, Ar" uniqKey="Mendes Pontes A">AR Mendes Pontes</name></author><author><name sortKey="Normande, Ic" uniqKey="Normande I">IC Normande</name></author><author><name sortKey="Fernandes, Aca" uniqKey="Fernandes A">ACA Fernandes</name></author><author><name sortKey="Rosas Ribeiro, Pf" uniqKey="Rosas Ribeiro P">PF Rosas Ribeiro</name></author><author><name sortKey="Soares, Ml" uniqKey="Soares M">ML Soares</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chalmers, Dt" uniqKey="Chalmers D">DT Chalmers</name></author><author><name sortKey="Murgatroyd, Lb" uniqKey="Murgatroyd L">LB Murgatroyd</name></author><author><name sortKey="Wadsworth, Pf" uniqKey="Wadsworth P">PF Wadsworth</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Truman, Rw" uniqKey="Truman R">RW Truman</name></author><author><name sortKey="Krahenbuhl, Jl" uniqKey="Krahenbuhl J">JL Krahenbuhl</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sambrook, J" uniqKey="Sambrook J">J Sambrook</name></author><author><name sortKey="Russell, Dw" uniqKey="Russell D">DW Russell</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Moura, Rs" uniqKey="Moura R">RS Moura</name></author><author><name sortKey="Calado, Kl" uniqKey="Calado K">KL Calado</name></author><author><name sortKey="Oliveira, Mlw" uniqKey="Oliveira M">MLW Oliveira</name></author><author><name sortKey="Buhrer Sekula, S" uniqKey="Buhrer Sekula S">S Bührer-Sékula</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Duthie, Ms" uniqKey="Duthie M">MS Duthie</name></author><author><name sortKey="Goto, W" uniqKey="Goto W">W Goto</name></author><author><name sortKey="Ireton, Gc" uniqKey="Ireton G">GC Ireton</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Malukiewicz, J" uniqKey="Malukiewicz J">J Malukiewicz</name></author><author><name sortKey="Boere, V" uniqKey="Boere V">V Boere</name></author><author><name sortKey="Fuzessy, Lf" uniqKey="Fuzessy L">LF Fuzessy</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Meyer, M" uniqKey="Meyer M">M Meyer</name></author><author><name sortKey="Kircher, M" uniqKey="Kircher M">M Kircher</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kircher, M" uniqKey="Kircher M">M Kircher</name></author><author><name sortKey="Sawyer, S" uniqKey="Sawyer S">S Sawyer</name></author><author><name sortKey="Meyer, M" uniqKey="Meyer M">M Meyer</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Maricic, T" uniqKey="Maricic T">T Maricic</name></author><author><name sortKey="Whitten, M" uniqKey="Whitten M">M Whitten</name></author><author><name sortKey="P Bo, S" uniqKey="P Bo S">S Pääbo</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Li, H" uniqKey="Li H">H Li</name></author><author><name sortKey="Durbin, R" uniqKey="Durbin R">R Durbin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Huson, Dh" uniqKey="Huson D">DH Huson</name></author><author><name sortKey="Auch, Af" uniqKey="Auch A">AF Auch</name></author><author><name sortKey="Qi, J" uniqKey="Qi J">J Qi</name></author><author><name sortKey="Schuster, Sc" uniqKey="Schuster S">SC Schuster</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Schuenemann, Vj" uniqKey="Schuenemann V">VJ Schuenemann</name></author><author><name sortKey="Singh, P" uniqKey="Singh P">P Singh</name></author><author><name sortKey="Mendum, Ta" uniqKey="Mendum T">TA Mendum</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Patrocinio, Lg" uniqKey="Patrocinio L">LG Patrocinio</name></author><author><name sortKey="Goulart, Imb" uniqKey="Goulart I">IMB Goulart</name></author><author><name sortKey="Goulart, Lr" uniqKey="Goulart L">LR Goulart</name></author><author><name sortKey="Patrocinio, Ja" uniqKey="Patrocinio J">JA Patrocinio</name></author><author><name sortKey="Ferreira, Fr" uniqKey="Ferreira F">FR Ferreira</name></author><author><name sortKey="Fleury, Rn" uniqKey="Fleury R">RN Fleury</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Da Rosa, Fb" uniqKey="Da Rosa F">FB da Rosa</name></author><author><name sortKey="De Souza, Vc" uniqKey="De Souza V">VC de Souza</name></author><author><name sortKey="De Almeida, Tap" uniqKey="De Almeida T">TAP de Almeida</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="De Almeida, Ec" uniqKey="De Almeida E">EC de Almeida</name></author><author><name sortKey="Martinez, An" uniqKey="Martinez A">AN Martinez</name></author><author><name sortKey="Maniero, Vc" uniqKey="Maniero V">VC Maniero</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Sharma, R" uniqKey="Sharma R">R Sharma</name></author><author><name sortKey="Lahiri, R" uniqKey="Lahiri R">R Lahiri</name></author><author><name sortKey="Scollard, Dm" uniqKey="Scollard D">DM Scollard</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Drummond, A" uniqKey="Drummond A">A Drummond</name></author><author><name sortKey="Nicholls, Gk" uniqKey="Nicholls G">GK Nicholls</name></author><author><name sortKey="Rodrigo, Ag" uniqKey="Rodrigo A">AG Rodrigo</name></author><author><name sortKey="Solomon, W" uniqKey="Solomon W">W Solomon</name></author><author><name sortKey="Buck, Ce" uniqKey="Buck C">CE Buck</name></author><author><name sortKey="Millard, A" uniqKey="Millard A">A Millard</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ginolhac, A" uniqKey="Ginolhac A">A Ginolhac</name></author><author><name sortKey="Rasmussen, M" uniqKey="Rasmussen M">M Rasmussen</name></author><author><name sortKey="Gilbert, Mt" uniqKey="Gilbert M">MT Gilbert</name></author><author><name sortKey="Willerslev, E" uniqKey="Willerslev E">E Willerslev</name></author><author><name sortKey="Orlando, L" uniqKey="Orlando L">L Orlando</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Martinez, An" uniqKey="Martinez A">AN Martinez</name></author><author><name sortKey="Talhari, C" uniqKey="Talhari C">C Talhari</name></author><author><name sortKey="Moraes, Mo" uniqKey="Moraes M">MO Moraes</name></author><author><name sortKey="Talhari, S" uniqKey="Talhari S">S Talhari</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Beyene, D" uniqKey="Beyene D">D Beyene</name></author><author><name sortKey="Aseffa, A" uniqKey="Aseffa A">A Aseffa</name></author><author><name sortKey="Harboe, M" uniqKey="Harboe M">M Harboe</name></author></analytic></biblStruct></listBibl></div1></back></TEI><pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">PLoS Negl Trop Dis</journal-id><journal-id journal-id-type="iso-abbrev">PLoS Negl Trop Dis</journal-id><journal-id journal-id-type="publisher-id">plos</journal-id><journal-id journal-id-type="pmc">plosntds</journal-id><journal-title-group><journal-title>PLoS Neglected Tropical Diseases</journal-title></journal-title-group><issn pub-type="ppub">1935-2727</issn><issn pub-type="epub">1935-2735</issn><publisher><publisher-name>Public Library of Science</publisher-name><publisher-loc>San Francisco, CA USA</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">26571269</article-id><article-id pub-id-type="pmc">4646627</article-id><article-id pub-id-type="doi">10.1371/journal.pntd.0004198</article-id><article-id pub-id-type="publisher-id">PNTD-D-15-00927</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group></article-categories><title-group><article-title>Validation of qPCR Methods for the Detection of <italic>Mycobacterium</italic> in New World Animal Reservoirs</article-title><alt-title alt-title-type="running-head">Detection of <italic>Mycobacterium</italic> in New World Animals</alt-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Housman</surname><given-names>Genevieve</given-names></name><xref ref-type="aff" rid="aff001"><sup>1</sup></xref><xref rid="cor001" ref-type="corresp">*</xref></contrib><contrib contrib-type="author"><name><surname>Malukiewicz</surname><given-names>Joanna</given-names></name><xref ref-type="aff" rid="aff001"><sup>1</sup></xref><xref ref-type="aff" rid="aff002"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>Boere</surname><given-names>Vanner</given-names></name><xref ref-type="aff" rid="aff002"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>Grativol</surname><given-names>Adriana D.</given-names></name><xref ref-type="aff" rid="aff003"><sup>3</sup></xref></contrib><contrib contrib-type="author"><name><surname>Pereira</surname><given-names>Luiz Cezar M.</given-names></name><xref ref-type="aff" rid="aff004"><sup>4</sup></xref></contrib><contrib contrib-type="author"><name><surname>Silva</surname><given-names>Ita de Oliveira e</given-names></name><xref ref-type="aff" rid="aff005"><sup>5</sup></xref></contrib><contrib contrib-type="author"><name><surname>Ruiz-Miranda</surname><given-names>Carlos R.</given-names></name><xref ref-type="aff" rid="aff003"><sup>3</sup></xref></contrib><contrib contrib-type="author"><name><surname>Truman</surname><given-names>Richard</given-names></name><xref ref-type="aff" rid="aff006"><sup>6</sup></xref></contrib><contrib contrib-type="author"><name><surname>Stone</surname><given-names>Anne C.</given-names></name><xref ref-type="aff" rid="aff001"><sup>1</sup></xref></contrib></contrib-group><aff id="aff001"><label>1</label><addr-line>School of Human Evolution and Social Change, Arizona State University, Tempe, Arizona, United States of America</addr-line></aff><aff id="aff002"><label>2</label><addr-line>Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brazil</addr-line></aff><aff id="aff003"><label>3</label><addr-line>Laboratório de Ciências Ambientias, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense, Campos dos Goytacazes, Rio de Janeiro, Brazil</addr-line></aff><aff id="aff004"><label>4</label><addr-line>Centro de Conservação e Manejo de Fauna da Caatinga, Universidade Federal do Vale do São Francisco, Petrolina, Pernambuco, Brazil</addr-line></aff><aff id="aff005"><label>5</label><addr-line>Departamento de Biologia Animal, Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brazil</addr-line></aff><aff id="aff006"><label>6</label><addr-line>HHS\HRSA\HSB National Hansen's Disease Program-NIAID IAA-2646, Baton Rouge, Louisiana, United States of America</addr-line></aff><contrib-group><contrib contrib-type="editor"><name><surname>Small</surname><given-names>Pamela L. C.</given-names></name><role>Editor</role><xref ref-type="aff" rid="edit1"></xref></contrib></contrib-group><aff id="edit1"><addr-line>University of Tennessee, UNITED STATES</addr-line></aff><author-notes><fn fn-type="conflict" id="coi001"><p>The authors have declared that no competing interests exist.</p></fn><fn fn-type="con" id="contrib001"><p>Conceived and designed the experiments: GH RT ACS. Performed the experiments: GH RT. Analyzed the data: GH. Contributed reagents/materials/analysis tools: JM VB ADG LCMP IdOeS CRRM RT ACS. Wrote the paper: GH.</p></fn><corresp id="cor001">* E-mail: <email>ghousman@asu.edu</email></corresp></author-notes><pub-date pub-type="epub"><day>16</day><month>11</month><year>2015</year></pub-date><pub-date pub-type="collection"><month>11</month><year>2015</year></pub-date><volume>9</volume><issue>11</issue><elocation-id>e0004198</elocation-id><history><date date-type="received"><day>30</day><month>5</month><year>2015</year></date><date date-type="accepted"><day>6</day><month>10</month><year>2015</year></date></history><permissions><license xlink:href="https://creativecommons.org/publicdomain/zero/1.0/"><license-p>This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the <ext-link ext-link-type="uri" xlink:href="https://creativecommons.org/publicdomain/zero/1.0/">Creative Commons CC0</ext-link> public domain dedication</license-p></license></permissions><self-uri content-type="pdf" xlink:type="simple" xlink:href="pntd.0004198.pdf"></self-uri><abstract><p>Zoonotic pathogens that cause leprosy (<italic>Mycobacterium leprae</italic>) and tuberculosis (<italic>Mycobacterium tuberculosis</italic> complex, MTBC) continue to impact modern human populations. Therefore, methods able to survey mycobacterial infection in potential animal hosts are necessary for proper evaluation of human exposure threats. Here we tested for mycobacterial-specific single- and multi-copy loci using qPCR. In a trial study in which armadillos were artificially infected with <italic>M</italic>. <italic>leprae</italic>, these techniques were specific and sensitive to pathogen detection, while more traditional ELISAs were only specific. These assays were then employed in a case study to detect <italic>M</italic>. <italic>leprae</italic> as well as MTBC in wild marmosets. All marmosets were negative for <italic>M</italic>. <italic>leprae</italic> DNA, but 14 were positive for the mycobacterial rpoB gene assay. Targeted capture and sequencing of rpoB and other MTBC genes validated the presence of mycobacterial DNA in these samples and revealed that qPCR is useful for identifying mycobacterial-infected animal hosts.</p></abstract><abstract abstract-type="summary"><title>Author Summary</title><p>Mycobacterial pathogens that cause tuberculosis and leprosy can be detected in wild animal populations using non-invasive cheek swab samples and quantitative polymerase chain reaction assays that target specific portions of mycobacterial genomes. A preliminary study in armadillos confirms that using multiple assays in tandem is optimal for surveying infection in animals, and a case study identifies some mycobacterial infection in wild marmosets from Brazil. These validated methods can be used to advance our understanding of the distribution and impact of zoonotic pathogens on wild animal populations.</p></abstract><funding-group><funding-statement>This work was supported by the National Institute of Allergy and Infectious Diseases (<ext-link ext-link-type="uri" xlink:href="http://www.niaid.nih.gov">www.niaid.nih.gov</ext-link>) [NIAID IAA-2646 to RT]; the National Science Foundation (<ext-link ext-link-type="uri" xlink:href="http://www.nsf.gov">www.nsf.gov</ext-link>) [NSF BCS-1063939 to ACS and NSF DDIG-1061508 to JM]; the Fulbright Scholar Program (<ext-link ext-link-type="uri" xlink:href="http://www.cies.org">www.cies.org</ext-link>) [Fellowship to JM]; the International Primatological Society (<ext-link ext-link-type="uri" xlink:href="http://www.internationalprimatologicalsociety.org">www.internationalprimatologicalsociety.org</ext-link>) [Research Grant to JM]; the Arizona State University Chapter of Sigma Xi (sigmaxi.asu.edu) [GIAR to JM]; and the Arizona State University Graduate and Professional Student Association (gpsa.asu.edu) [Jumpstart and Research Grant to JM]. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.</funding-statement></funding-group><counts><fig-count count="4"></fig-count><table-count count="3"></table-count><page-count count="13"></page-count></counts><custom-meta-group><custom-meta id="data-availability"><meta-name>Data Availability</meta-name><meta-value>The next-generation sequencing reads produced during this project did not align to the predicted reference sequences and were unable to be converted into consensus sequences. Because of this, we have not submitted them to GenBank. However, the reads have been uploaded as <xref rid="pntd.0004198.s002" ref-type="supplementary-material">S1 Dataset</xref>. All other relevant data are within the paper.</meta-value></custom-meta></custom-meta-group></article-meta><notes><title>Data Availability</title><p>The next-generation sequencing reads produced during this project did not align to the predicted reference sequences and were unable to be converted into consensus sequences. Because of this, we have not submitted them to GenBank. However, the reads have been uploaded as <xref rid="pntd.0004198.s002" ref-type="supplementary-material">S1 Dataset</xref>. All other relevant data are within the paper.</p></notes></front></pmc><affiliations><list><country><li>Brésil</li><li>États-Unis</li></country><region><li>Arizona</li><li>Louisiane</li><li>Minas Gerais</li><li>Pernambuco</li><li>État de Rio de Janeiro</li></region><settlement><li>Rio de Janeiro</li></settlement></list><tree><country name="États-Unis"><region name="Arizona"><name sortKey="Housman, Genevieve" sort="Housman, Genevieve" uniqKey="Housman G" first="Genevieve" last="Housman">Genevieve Housman</name></region><name sortKey="Malukiewicz, Joanna" sort="Malukiewicz, Joanna" uniqKey="Malukiewicz J" first="Joanna" last="Malukiewicz">Joanna Malukiewicz</name><name sortKey="Stone, Anne C" sort="Stone, Anne C" uniqKey="Stone A" first="Anne C." last="Stone">Anne C. Stone</name><name sortKey="Truman, Richard" sort="Truman, Richard" uniqKey="Truman R" first="Richard" last="Truman">Richard Truman</name></country><country name="Brésil"><region name="Minas Gerais"><name sortKey="Malukiewicz, Joanna" sort="Malukiewicz, Joanna" uniqKey="Malukiewicz J" first="Joanna" last="Malukiewicz">Joanna Malukiewicz</name></region><name sortKey="Boere, Vanner" sort="Boere, Vanner" uniqKey="Boere V" first="Vanner" last="Boere">Vanner Boere</name><name sortKey="Grativol, Adriana D" sort="Grativol, Adriana D" uniqKey="Grativol A" first="Adriana D." last="Grativol">Adriana D. Grativol</name><name sortKey="Pereira, Luiz Cezar M" sort="Pereira, Luiz Cezar M" uniqKey="Pereira L" first="Luiz Cezar M." last="Pereira">Luiz Cezar M. Pereira</name><name sortKey="Ruiz Miranda, Carlos R" sort="Ruiz Miranda, Carlos R" uniqKey="Ruiz Miranda C" first="Carlos R." last="Ruiz-Miranda">Carlos R. Ruiz-Miranda</name><name sortKey="Silva, Ita De Oliveira E" sort="Silva, Ita De Oliveira E" uniqKey="Silva I" first="Ita De Oliveira E" last="Silva">Ita De Oliveira E Silva</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Archeologie/explor/PaleopathV1/Data/Pmc/Checkpoint
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000029 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Pmc/Checkpoint/biblio.hfd -nk 000029 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Archeologie
|area= PaleopathV1
|flux= Pmc
|étape= Checkpoint
|type= RBID
|clé= PMC:4646627
|texte= Validation of qPCR Methods for the Detection of Mycobacterium in New World Animal Reservoirs
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Pmc/Checkpoint/RBID.i -Sk "pubmed:26571269" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Pmc/Checkpoint/biblio.hfd \
| NlmPubMed2Wicri -a PaleopathV1
| This area was generated with Dilib version V0.6.27. |  |