Validation of qPCR Methods for the Detection of Mycobacterium in New World Animal Reservoirs
Identifieur interne : 000F27 ( Ncbi/Curation ); précédent : 000F26; suivant : 000F28Validation of qPCR Methods for the Detection of Mycobacterium in New World Animal Reservoirs
Auteurs : Genevieve Housman [États-Unis] ; Joanna Malukiewicz [États-Unis, Brésil] ; Vanner Boere [Brésil] ; Adriana D. Grativol [Brésil] ; Luiz Cezar M. Pereira [Brésil] ; Ita De Oliveira E Silva [Brésil] ; Carlos R. Ruiz-Miranda [Brésil] ; Richard Truman [États-Unis] ; Anne C. Stone [États-Unis]Source :
- PLoS Neglected Tropical Diseases [ 1935-2727 ] ; 2015.
Abstract
Zoonotic pathogens that cause leprosy (
Url:
DOI: 10.1371/journal.pntd.0004198
PubMed: 26571269
PubMed Central: 4646627
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PMC:4646627Le document en format XML
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<author><name sortKey="Malukiewicz, Joanna" sort="Malukiewicz, Joanna" uniqKey="Malukiewicz J" first="Joanna" last="Malukiewicz">Joanna Malukiewicz</name>
<affiliation wicri:level="2"><nlm:aff id="aff001"><addr-line>School of Human Evolution and Social Change, Arizona State University, Tempe, Arizona, United States of America</addr-line>
</nlm:aff>
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<wicri:regionArea>School of Human Evolution and Social Change, Arizona State University, Tempe, Arizona</wicri:regionArea>
<placeName><region type="state">Arizona</region>
</placeName>
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</nlm:aff>
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<wicri:regionArea>Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Viçosa, Viçosa, Minas Gerais</wicri:regionArea>
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<author><name sortKey="Boere, Vanner" sort="Boere, Vanner" uniqKey="Boere V" first="Vanner" last="Boere">Vanner Boere</name>
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</nlm:aff>
<country xml:lang="fr">Brésil</country>
<wicri:regionArea>Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Viçosa, Viçosa, Minas Gerais</wicri:regionArea>
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</author>
<author><name sortKey="Grativol, Adriana D" sort="Grativol, Adriana D" uniqKey="Grativol A" first="Adriana D." last="Grativol">Adriana D. Grativol</name>
<affiliation wicri:level="3"><nlm:aff id="aff003"><addr-line>Laboratório de Ciências Ambientias, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense, Campos dos Goytacazes, Rio de Janeiro, Brazil</addr-line>
</nlm:aff>
<country xml:lang="fr">Brésil</country>
<wicri:regionArea>Laboratório de Ciências Ambientias, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense, Campos dos Goytacazes, Rio de Janeiro</wicri:regionArea>
<placeName><settlement type="city">Rio de Janeiro</settlement>
<region type="state">État de Rio de Janeiro</region>
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<author><name sortKey="Pereira, Luiz Cezar M" sort="Pereira, Luiz Cezar M" uniqKey="Pereira L" first="Luiz Cezar M." last="Pereira">Luiz Cezar M. Pereira</name>
<affiliation wicri:level="2"><nlm:aff id="aff004"><addr-line>Centro de Conservação e Manejo de Fauna da Caatinga, Universidade Federal do Vale do São Francisco, Petrolina, Pernambuco, Brazil</addr-line>
</nlm:aff>
<country xml:lang="fr">Brésil</country>
<wicri:regionArea>Centro de Conservação e Manejo de Fauna da Caatinga, Universidade Federal do Vale do São Francisco, Petrolina, Pernambuco</wicri:regionArea>
<placeName><region type="state">Pernambuco</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Silva, Ita De Oliveira E" sort="Silva, Ita De Oliveira E" uniqKey="Silva I" first="Ita De Oliveira E" last="Silva">Ita De Oliveira E Silva</name>
<affiliation wicri:level="2"><nlm:aff id="aff005"><addr-line>Departamento de Biologia Animal, Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brazil</addr-line>
</nlm:aff>
<country xml:lang="fr">Brésil</country>
<wicri:regionArea>Departamento de Biologia Animal, Universidade Federal de Viçosa, Viçosa, Minas Gerais</wicri:regionArea>
<placeName><region type="state">Minas Gerais</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Ruiz Miranda, Carlos R" sort="Ruiz Miranda, Carlos R" uniqKey="Ruiz Miranda C" first="Carlos R." last="Ruiz-Miranda">Carlos R. Ruiz-Miranda</name>
<affiliation wicri:level="3"><nlm:aff id="aff003"><addr-line>Laboratório de Ciências Ambientias, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense, Campos dos Goytacazes, Rio de Janeiro, Brazil</addr-line>
</nlm:aff>
<country xml:lang="fr">Brésil</country>
<wicri:regionArea>Laboratório de Ciências Ambientias, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense, Campos dos Goytacazes, Rio de Janeiro</wicri:regionArea>
<placeName><settlement type="city">Rio de Janeiro</settlement>
<region type="state">État de Rio de Janeiro</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Truman, Richard" sort="Truman, Richard" uniqKey="Truman R" first="Richard" last="Truman">Richard Truman</name>
<affiliation wicri:level="2"><nlm:aff id="aff006"><addr-line>HHS\HRSA\HSB National Hansen's Disease Program-NIAID IAA-2646, Baton Rouge, Louisiana, United States of America</addr-line>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>HHS\HRSA\HSB National Hansen's Disease Program-NIAID IAA-2646, Baton Rouge, Louisiana</wicri:regionArea>
<placeName><region type="state">Louisiane</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Stone, Anne C" sort="Stone, Anne C" uniqKey="Stone A" first="Anne C." last="Stone">Anne C. Stone</name>
<affiliation wicri:level="2"><nlm:aff id="aff001"><addr-line>School of Human Evolution and Social Change, Arizona State University, Tempe, Arizona, United States of America</addr-line>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>School of Human Evolution and Social Change, Arizona State University, Tempe, Arizona</wicri:regionArea>
<placeName><region type="state">Arizona</region>
</placeName>
</affiliation>
</author>
</analytic>
<series><title level="j">PLoS Neglected Tropical Diseases</title>
<idno type="ISSN">1935-2727</idno>
<idno type="eISSN">1935-2735</idno>
<imprint><date when="2015">2015</date>
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<front><div type="abstract" xml:lang="en"><p>Zoonotic pathogens that cause leprosy (<italic>Mycobacterium leprae</italic>
) and tuberculosis (<italic>Mycobacterium tuberculosis</italic>
complex, MTBC) continue to impact modern human populations. Therefore, methods able to survey mycobacterial infection in potential animal hosts are necessary for proper evaluation of human exposure threats. Here we tested for mycobacterial-specific single- and multi-copy loci using qPCR. In a trial study in which armadillos were artificially infected with <italic>M</italic>
. <italic>leprae</italic>
, these techniques were specific and sensitive to pathogen detection, while more traditional ELISAs were only specific. These assays were then employed in a case study to detect <italic>M</italic>
. <italic>leprae</italic>
as well as MTBC in wild marmosets. All marmosets were negative for <italic>M</italic>
. <italic>leprae</italic>
DNA, but 14 were positive for the mycobacterial rpoB gene assay. Targeted capture and sequencing of rpoB and other MTBC genes validated the presence of mycobacterial DNA in these samples and revealed that qPCR is useful for identifying mycobacterial-infected animal hosts.</p>
</div>
</front>
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</author>
</analytic>
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<biblStruct><analytic><author><name sortKey="Wolfe, Nd" uniqKey="Wolfe N">ND Wolfe</name>
</author>
<author><name sortKey="Dunavan, Cp" uniqKey="Dunavan C">CP Dunavan</name>
</author>
<author><name sortKey="Diamond, J" uniqKey="Diamond J">J Diamond</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Zakham, F" uniqKey="Zakham F">F Zakham</name>
</author>
<author><name sortKey="Aouane, O" uniqKey="Aouane O">O Aouane</name>
</author>
<author><name sortKey="Ussery, D" uniqKey="Ussery D">D Ussery</name>
</author>
<author><name sortKey="Benjouad, A" uniqKey="Benjouad A">A Benjouad</name>
</author>
<author><name sortKey="Ennaji, Mm" uniqKey="Ennaji M">MM Ennaji</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Klaus, Hd" uniqKey="Klaus H">HD Klaus</name>
</author>
<author><name sortKey="Wilbur, Ak" uniqKey="Wilbur A">AK Wilbur</name>
</author>
<author><name sortKey="Temple, Dh" uniqKey="Temple D">DH Temple</name>
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</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Wilbur, Ak" uniqKey="Wilbur A">AK Wilbur</name>
</author>
<author><name sortKey="Engel, Ga" uniqKey="Engel G">GA Engel</name>
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<author><name sortKey="Rompis, A" uniqKey="Rompis A">A Rompis</name>
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</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Montali, Rj" uniqKey="Montali R">RJ Montali</name>
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<author><name sortKey="Cheng, Li" uniqKey="Cheng L">LI Cheng</name>
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</analytic>
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<biblStruct><analytic><author><name sortKey="Brusasca, Pn" uniqKey="Brusasca P">PN Brusasca</name>
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<author><name sortKey="Peters, Rl" uniqKey="Peters R">RL Peters</name>
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<author><name sortKey="Motzel, Sl" uniqKey="Motzel S">SL Motzel</name>
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</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Griffith, S" uniqKey="Griffith S">S Griffith</name>
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</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Truman, Rw" uniqKey="Truman R">RW Truman</name>
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<author><name sortKey="Britto, Cfpc" uniqKey="Britto C">CFPC Britto</name>
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<author><name sortKey="Nery, Jac" uniqKey="Nery J">JAC Nery</name>
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</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Harkins, Km" uniqKey="Harkins K">KM Harkins</name>
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<author><name sortKey="Buikstra, Je" uniqKey="Buikstra J">JE Buikstra</name>
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</biblStruct>
<biblStruct><analytic><author><name sortKey="Thorne, N" uniqKey="Thorne N">N Thorne</name>
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