New finding of Giardia intestinalis (Eukaryote, Metamonad) in Old World archaeological site using immunofluorescence and enzyme-linked immunosorbent assays.
Identifieur interne : 000860 ( Ncbi/Checkpoint ); précédent : 000859; suivant : 000861New finding of Giardia intestinalis (Eukaryote, Metamonad) in Old World archaeological site using immunofluorescence and enzyme-linked immunosorbent assays.
Auteurs : Matthieu Le Bailly [France] ; Marcelo Lc Gonçalves ; Stéphanie Harter-Lailheugue ; Frédéric Prodéo ; Adauto Araujo ; Françoise BouchetSource :
- Memorias do Instituto Oswaldo Cruz [ 0074-0276 ] ; 2008.
Descripteurs français
- Wicri :
- geographic : France.
English descriptors
- KwdEn :
- MESH :
- geographic : France.
- isolation & purification : Giardia.
- parasitology : Feces.
- Animals, Enzyme-Linked Immunosorbent Assay, Fluorescent Antibody Technique, Humans, Paleopathology.
Abstract
In this study, nine organic sediment samples from a medieval archaeological site at Pineuilh, France, were examined for Giardia intestinalis using two commercially available immunological kits [enzyme-linked immuno sorbent and immunofluorescence (IFA) assays]. Both techniques detected G. intestinalis in one sample, dated to 1,000 Anno Domini. This is the first time IFA was successfully used to detect protozoa in Old World archaeological samples. Such immunological techniques offer important perspectives concerning ancient protozoa detection and identification.
PubMed: 18545853
Affiliations:
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first="Adauto" last="Araujo">Adauto Araujo</name></author><author><name sortKey="Bouchet, Francoise" sort="Bouchet, Francoise" uniqKey="Bouchet F" first="Françoise" last="Bouchet">Françoise Bouchet</name></author></analytic><series><title level="j">Memorias do Instituto Oswaldo Cruz</title><idno type="ISSN">0074-0276</idno><imprint><date when="2008" type="published">2008</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Feces (parasitology)</term><term>Fluorescent Antibody Technique</term><term>France</term><term>Giardia (isolation & purification)</term><term>Humans</term><term>Paleopathology</term></keywords><keywords scheme="MESH" type="geographic" xml:lang="en"><term>France</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Giardia</term></keywords><keywords scheme="MESH" qualifier="parasitology" xml:lang="en"><term>Feces</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Fluorescent Antibody Technique</term><term>Humans</term><term>Paleopathology</term></keywords><keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>France</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In this study, nine organic sediment samples from a medieval archaeological site at Pineuilh, France, were examined for Giardia intestinalis using two commercially available immunological kits [enzyme-linked immuno sorbent and immunofluorescence (IFA) assays]. Both techniques detected G. intestinalis in one sample, dated to 1,000 Anno Domini. This is the first time IFA was successfully used to detect protozoa in Old World archaeological samples. 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