New finding of Giardia intestinalis (Eukaryote, Metamonad) in Old World archaeological site using immunofluorescence and enzyme-linked immunosorbent assays.
Identifieur interne : 000477 ( PubMed/Corpus ); précédent : 000476; suivant : 000478New finding of Giardia intestinalis (Eukaryote, Metamonad) in Old World archaeological site using immunofluorescence and enzyme-linked immunosorbent assays.
Auteurs : Matthieu Le Bailly ; Marcelo Lc Gonçalves ; Stéphanie Harter-Lailheugue ; Frédéric Prodéo ; Adauto Araujo ; Françoise BouchetSource :
- Memorias do Instituto Oswaldo Cruz [ 0074-0276 ] ; 2008.
English descriptors
- KwdEn :
- MESH :
- geographic : France.
- isolation & purification : Giardia.
- parasitology : Feces.
- Animals, Enzyme-Linked Immunosorbent Assay, Fluorescent Antibody Technique, Humans, Paleopathology.
Abstract
In this study, nine organic sediment samples from a medieval archaeological site at Pineuilh, France, were examined for Giardia intestinalis using two commercially available immunological kits [enzyme-linked immuno sorbent and immunofluorescence (IFA) assays]. Both techniques detected G. intestinalis in one sample, dated to 1,000 Anno Domini. This is the first time IFA was successfully used to detect protozoa in Old World archaeological samples. Such immunological techniques offer important perspectives concerning ancient protozoa detection and identification.
PubMed: 18545853
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pubmed:18545853Le document en format XML
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Both techniques detected G. intestinalis in one sample, dated to 1,000 Anno Domini. This is the first time IFA was successfully used to detect protozoa in Old World archaeological samples. Such immunological techniques offer important perspectives concerning ancient protozoa detection and identification.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">18545853</PMID><DateCreated><Year>2008</Year><Month>07</Month><Day>01</Day></DateCreated><DateCompleted><Year>2008</Year><Month>12</Month><Day>29</Day></DateCompleted><DateRevised><Year>2008</Year><Month>07</Month><Day>01</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0074-0276</ISSN><JournalIssue CitedMedium="Print"><Volume>103</Volume><Issue>3</Issue><PubDate><Year>2008</Year><Month>May</Month></PubDate></JournalIssue><Title>Memorias do Instituto Oswaldo Cruz</Title><ISOAbbreviation>Mem. Inst. Oswaldo Cruz</ISOAbbreviation></Journal><ArticleTitle>New finding of Giardia intestinalis (Eukaryote, Metamonad) in Old World archaeological site using immunofluorescence and enzyme-linked immunosorbent assays.</ArticleTitle><Pagination><MedlinePgn>298-300</MedlinePgn></Pagination><ELocationID EIdType="pii" ValidYN="Y">S0074-02762008005000018</ELocationID><Abstract><AbstractText>In this study, nine organic sediment samples from a medieval archaeological site at Pineuilh, France, were examined for Giardia intestinalis using two commercially available immunological kits [enzyme-linked immuno sorbent and immunofluorescence (IFA) assays]. Both techniques detected G. intestinalis in one sample, dated to 1,000 Anno Domini. This is the first time IFA was successfully used to detect protozoa in Old World archaeological samples. Such immunological techniques offer important perspectives concerning ancient protozoa detection and identification.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Le Bailly</LastName><ForeName>Matthieu</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Laboratoire de Paléoparasitologie, UFR de Pharmacie, Université de Reims, Reims, France. matthieu.lebailly@univ-reims.fr</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gonçalves</LastName><ForeName>Marcelo Lc</ForeName><Initials>ML</Initials></Author><Author ValidYN="Y"><LastName>Harter-Lailheugue</LastName><ForeName>Stéphanie</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Prodéo</LastName><ForeName>Frédéric</ForeName><Initials>F</Initials></Author><Author ValidYN="Y"><LastName>Araujo</LastName><ForeName>Adauto</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Bouchet</LastName><ForeName>Françoise</ForeName><Initials>F</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2008</Year><Month>04</Month><Day>30</Day></ArticleDate></Article><MedlineJournalInfo><Country>Brazil</Country><MedlineTA>Mem Inst Oswaldo Cruz</MedlineTA><NlmUniqueID>7502619</NlmUniqueID><ISSNLinking>0074-0276</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004797" MajorTopicYN="N">Enzyme-Linked Immunosorbent Assay</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005243" MajorTopicYN="N">Feces</DescriptorName><QualifierName UI="Q000469" MajorTopicYN="N">parasitology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005455" MajorTopicYN="N">Fluorescent Antibody Technique</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005602" MajorTopicYN="N" Type="Geographic">France</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005872" MajorTopicYN="N">Giardia</DescriptorName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010164" MajorTopicYN="Y">Paleopathology</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2008</Year><Month>02</Month><Day>27</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2008</Year><Month>05</Month><Day>08</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2008</Year><Month>6</Month><Day>12</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2008</Year><Month>12</Month><Day>30</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2008</Year><Month>6</Month><Day>12</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">18545853</ArticleId><ArticleId IdType="pii">S0074-02762008005000018</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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