Biopsies of olfactory epithelium in patients with Parkinson's disease
Identifieur interne : 000497 ( Istex/Corpus ); précédent : 000496; suivant : 000498Biopsies of olfactory epithelium in patients with Parkinson's disease
Auteurs : Martin Witt ; Katja Bormann ; Volker Gudziol ; Kerstin Pehlke ; Kathrin Barth ; Amir Minovi ; Antje H Hner ; Heinz Reichmann ; Thomas HummelSource :
- Movement Disorders [ 0885-3185 ] ; 2009-04-30.
English descriptors
- KwdEn :
Abstract
Parkinson's disease (PD) is a neurodegenerative disorder involving several neuronal systems. Impaired olfactory function may constitute one of the earliest symptoms of PD. However, it is still unclear to what degree changes of the olfactory epithelium may contribute to dysosmia and if these changes are different from those of other hyposmic or anosmic patients. This study aimed to investigate the hypothesis that olfactory loss in PD is a consequence of specific PD‐related damage of olfactory epithelium. Biopsies of 7 patients diagnosed with PD were taken. Six patients with PD were hyposmic, one anosmic. As non‐PD controls served 9 patients with hyposmia, 9 with anosmia, and 7 normosmic individuals. Further, nasal mucosa of 4 postmortem individuals was investigated. Immunohistochemical examinations were performed with antibodies against olfactory marker protein (OMP), protein gene product 9.5 (PGP 9.5), beta‐tubulin, (BT), proliferation‐associated antigen (Ki 67), the stem cell marker nestin, cytokeratin, p75NGFr, and α‐synuclein. Most of the biopsy specimens exhibited irregular areas of olfactory‐like, dysplastic epithelium positive for either PGP 9.5 or BT, but negative for OMP. No major histochemical differences in either the expression or distribution of these proteins were observed in the olfactory epithelium of patients with PD compared with controls. Reverse transcription PCR (RT‐PCR) data indicated mRNA for OMP in almost all subjects, independently of their olfactory performance. These data support the idea that olfactory loss in Parkinson's disease is not a consequence of damage to the olfactory epithelium but rather results from distinct central‐nervous abnormalities. © 2009 Movement Disorder Society
Url:
DOI: 10.1002/mds.22464
Links to Exploration step
ISTEX:0744835A9C2F9F6258CD0268AD01409B7A6B4805Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Biopsies of olfactory epithelium in patients with Parkinson's disease</title><author><name sortKey="Witt, Martin" sort="Witt, Martin" uniqKey="Witt M" first="Martin" last="Witt">Martin Witt</name><affiliation><mods:affiliation>Department of Anatomy, University of Rostock, 18057 Rostock, Germany</mods:affiliation></affiliation><affiliation><mods:affiliation>Department of Otorhinolaryngology, Interdisciplinary Center for Smell and Taste, University of Dresden Medical School, 01307 Dresden, Germany</mods:affiliation></affiliation></author><author><name sortKey="Bormann, Katja" sort="Bormann, Katja" uniqKey="Bormann K" first="Katja" last="Bormann">Katja Bormann</name><affiliation><mods:affiliation>Department of Anatomy, University of Dresden Medical School, 01307 Dresden, Germany</mods:affiliation></affiliation></author><author><name sortKey="Gudziol, Volker" sort="Gudziol, Volker" uniqKey="Gudziol V" first="Volker" last="Gudziol">Volker Gudziol</name><affiliation><mods:affiliation>Department of Otorhinolaryngology, Interdisciplinary Center for Smell and Taste, University of Dresden Medical School, 01307 Dresden, Germany</mods:affiliation></affiliation></author><author><name sortKey="Pehlke, Kerstin" sort="Pehlke, Kerstin" uniqKey="Pehlke K" first="Kerstin" last="Pehlke">Kerstin Pehlke</name><affiliation><mods:affiliation>Department of Anatomy, University of Dresden Medical School, 01307 Dresden, Germany</mods:affiliation></affiliation></author><author><name sortKey="Barth, Kathrin" sort="Barth, Kathrin" uniqKey="Barth K" first="Kathrin" last="Barth">Kathrin Barth</name><affiliation><mods:affiliation>Department of Anatomy, University of Dresden Medical School, 01307 Dresden, Germany</mods:affiliation></affiliation></author><author><name sortKey="Minovi, Amir" sort="Minovi, Amir" uniqKey="Minovi A" first="Amir" last="Minovi">Amir Minovi</name><affiliation><mods:affiliation>Department of Otorhinolaryngology, University of Bochum Medical School, 44787 Bochum, Germany</mods:affiliation></affiliation></author><author><name sortKey="H Hner, Antje" sort="H Hner, Antje" uniqKey="H Hner A" first="Antje" last="H Hner">Antje H Hner</name><affiliation><mods:affiliation>Department of Otorhinolaryngology, Interdisciplinary Center for Smell and Taste, University of Dresden Medical School, 01307 Dresden, Germany</mods:affiliation></affiliation><affiliation><mods:affiliation>Department of Neurology, University of Dresden Medical School, 01307 Dresden, Germany</mods:affiliation></affiliation></author><author><name sortKey="Reichmann, Heinz" sort="Reichmann, Heinz" uniqKey="Reichmann H" first="Heinz" last="Reichmann">Heinz Reichmann</name><affiliation><mods:affiliation>Department of Neurology, University of Dresden Medical School, 01307 Dresden, Germany</mods:affiliation></affiliation></author><author><name sortKey="Hummel, Thomas" sort="Hummel, Thomas" uniqKey="Hummel T" first="Thomas" last="Hummel">Thomas Hummel</name><affiliation><mods:affiliation>Department of Otorhinolaryngology, Interdisciplinary Center for Smell and Taste, University of Dresden Medical School, 01307 Dresden, Germany</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:0744835A9C2F9F6258CD0268AD01409B7A6B4805</idno><date when="2009" year="2009">2009</date><idno type="doi">10.1002/mds.22464</idno><idno type="url">https://api.istex.fr/document/0744835A9C2F9F6258CD0268AD01409B7A6B4805/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000497</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Biopsies of olfactory epithelium in patients with Parkinson's disease</title><author><name sortKey="Witt, Martin" sort="Witt, Martin" uniqKey="Witt M" first="Martin" last="Witt">Martin Witt</name><affiliation><mods:affiliation>Department of Anatomy, University of Rostock, 18057 Rostock, Germany</mods:affiliation></affiliation><affiliation><mods:affiliation>Department of Otorhinolaryngology, Interdisciplinary Center for Smell and Taste, University of Dresden Medical School, 01307 Dresden, Germany</mods:affiliation></affiliation></author><author><name sortKey="Bormann, Katja" sort="Bormann, Katja" uniqKey="Bormann K" first="Katja" last="Bormann">Katja Bormann</name><affiliation><mods:affiliation>Department of Anatomy, University of Dresden Medical School, 01307 Dresden, Germany</mods:affiliation></affiliation></author><author><name sortKey="Gudziol, Volker" sort="Gudziol, Volker" uniqKey="Gudziol V" first="Volker" last="Gudziol">Volker Gudziol</name><affiliation><mods:affiliation>Department of Otorhinolaryngology, Interdisciplinary Center for Smell and Taste, University of Dresden Medical School, 01307 Dresden, Germany</mods:affiliation></affiliation></author><author><name sortKey="Pehlke, Kerstin" sort="Pehlke, Kerstin" uniqKey="Pehlke K" first="Kerstin" last="Pehlke">Kerstin Pehlke</name><affiliation><mods:affiliation>Department of Anatomy, University of Dresden Medical School, 01307 Dresden, Germany</mods:affiliation></affiliation></author><author><name sortKey="Barth, Kathrin" sort="Barth, Kathrin" uniqKey="Barth K" first="Kathrin" last="Barth">Kathrin Barth</name><affiliation><mods:affiliation>Department of Anatomy, University of Dresden Medical School, 01307 Dresden, Germany</mods:affiliation></affiliation></author><author><name sortKey="Minovi, Amir" sort="Minovi, Amir" uniqKey="Minovi A" first="Amir" last="Minovi">Amir Minovi</name><affiliation><mods:affiliation>Department of Otorhinolaryngology, University of Bochum Medical School, 44787 Bochum, Germany</mods:affiliation></affiliation></author><author><name sortKey="H Hner, Antje" sort="H Hner, Antje" uniqKey="H Hner A" first="Antje" last="H Hner">Antje H Hner</name><affiliation><mods:affiliation>Department of Otorhinolaryngology, Interdisciplinary Center for Smell and Taste, University of Dresden Medical School, 01307 Dresden, Germany</mods:affiliation></affiliation><affiliation><mods:affiliation>Department of Neurology, University of Dresden Medical School, 01307 Dresden, Germany</mods:affiliation></affiliation></author><author><name sortKey="Reichmann, Heinz" sort="Reichmann, Heinz" uniqKey="Reichmann H" first="Heinz" last="Reichmann">Heinz Reichmann</name><affiliation><mods:affiliation>Department of Neurology, University of Dresden Medical School, 01307 Dresden, Germany</mods:affiliation></affiliation></author><author><name sortKey="Hummel, Thomas" sort="Hummel, Thomas" uniqKey="Hummel T" first="Thomas" last="Hummel">Thomas Hummel</name><affiliation><mods:affiliation>Department of Otorhinolaryngology, Interdisciplinary Center for Smell and Taste, University of Dresden Medical School, 01307 Dresden, Germany</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Movement Disorders</title><title level="j" type="sub">Official Journal of the Movement Disorder Society</title><title level="j" type="abbrev">Mov. Disord.</title><idno type="ISSN">0885-3185</idno><idno type="eISSN">1531-8257</idno><imprint><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><pubPlace>Hoboken</pubPlace><date type="published" when="2009-04-30">2009-04-30</date><biblScope unit="vol">24</biblScope><biblScope unit="issue">6</biblScope><biblScope unit="page" from="906">906</biblScope><biblScope unit="page" to="914">914</biblScope></imprint><idno type="ISSN">0885-3185</idno></series><idno type="istex">0744835A9C2F9F6258CD0268AD01409B7A6B4805</idno><idno type="DOI">10.1002/mds.22464</idno><idno type="ArticleID">MDS22464</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0885-3185</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>RT‐PCR</term><term>anosmia</term><term>hyposmia</term><term>immunohistochemistry</term><term>olfactory epithelium</term><term>smell</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Parkinson's disease (PD) is a neurodegenerative disorder involving several neuronal systems. Impaired olfactory function may constitute one of the earliest symptoms of PD. However, it is still unclear to what degree changes of the olfactory epithelium may contribute to dysosmia and if these changes are different from those of other hyposmic or anosmic patients. This study aimed to investigate the hypothesis that olfactory loss in PD is a consequence of specific PD‐related damage of olfactory epithelium. Biopsies of 7 patients diagnosed with PD were taken. Six patients with PD were hyposmic, one anosmic. As non‐PD controls served 9 patients with hyposmia, 9 with anosmia, and 7 normosmic individuals. Further, nasal mucosa of 4 postmortem individuals was investigated. Immunohistochemical examinations were performed with antibodies against olfactory marker protein (OMP), protein gene product 9.5 (PGP 9.5), beta‐tubulin, (BT), proliferation‐associated antigen (Ki 67), the stem cell marker nestin, cytokeratin, p75NGFr, and α‐synuclein. Most of the biopsy specimens exhibited irregular areas of olfactory‐like, dysplastic epithelium positive for either PGP 9.5 or BT, but negative for OMP. No major histochemical differences in either the expression or distribution of these proteins were observed in the olfactory epithelium of patients with PD compared with controls. Reverse transcription PCR (RT‐PCR) data indicated mRNA for OMP in almost all subjects, independently of their olfactory performance. These data support the idea that olfactory loss in Parkinson's disease is not a consequence of damage to the olfactory epithelium but rather results from distinct central‐nervous abnormalities. © 2009 Movement Disorder Society</div></front></TEI><istex><corpusName>wiley</corpusName><author><json:item><name>Martin Witt MD</name><affiliations><json:string>Department of Anatomy, University of Rostock, 18057 Rostock, Germany</json:string><json:string>Department of Otorhinolaryngology, Interdisciplinary Center for Smell and Taste, University of Dresden Medical School, 01307 Dresden, Germany</json:string></affiliations></json:item><json:item><name>Katja Bormann</name><affiliations><json:string>Department of Anatomy, University of Dresden Medical School, 01307 Dresden, Germany</json:string></affiliations></json:item><json:item><name>Volker Gudziol MD</name><affiliations><json:string>Department of Otorhinolaryngology, Interdisciplinary Center for Smell and Taste, University of Dresden Medical School, 01307 Dresden, Germany</json:string></affiliations></json:item><json:item><name>Kerstin Pehlke</name><affiliations><json:string>Department of Anatomy, University of Dresden Medical School, 01307 Dresden, Germany</json:string></affiliations></json:item><json:item><name>Kathrin Barth PhD</name><affiliations><json:string>Department of Anatomy, University of Dresden Medical School, 01307 Dresden, Germany</json:string></affiliations></json:item><json:item><name>Amir Minovi MD</name><affiliations><json:string>Department of Otorhinolaryngology, University of Bochum Medical School, 44787 Bochum, Germany</json:string></affiliations></json:item><json:item><name>Antje Hähner MD</name><affiliations><json:string>Department of Otorhinolaryngology, Interdisciplinary Center for Smell and Taste, University of Dresden Medical School, 01307 Dresden, Germany</json:string><json:string>Department of Neurology, University of Dresden Medical School, 01307 Dresden, Germany</json:string></affiliations></json:item><json:item><name>Heinz Reichmann MD</name><affiliations><json:string>Department of Neurology, University of Dresden Medical School, 01307 Dresden, Germany</json:string></affiliations></json:item><json:item><name>Thomas Hummel MD</name><affiliations><json:string>Department of Otorhinolaryngology, Interdisciplinary Center for Smell and Taste, University of Dresden Medical School, 01307 Dresden, Germany</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>anosmia</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>hyposmia</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>smell</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>olfactory epithelium</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>immunohistochemistry</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>RT‐PCR</value></json:item></subject><language><json:string>eng</json:string></language><abstract>Parkinson's disease (PD) is a neurodegenerative disorder involving several neuronal systems. Impaired olfactory function may constitute one of the earliest symptoms of PD. However, it is still unclear to what degree changes of the olfactory epithelium may contribute to dysosmia and if these changes are different from those of other hyposmic or anosmic patients. This study aimed to investigate the hypothesis that olfactory loss in PD is a consequence of specific PD‐related damage of olfactory epithelium. Biopsies of 7 patients diagnosed with PD were taken. Six patients with PD were hyposmic, one anosmic. As non‐PD controls served 9 patients with hyposmia, 9 with anosmia, and 7 normosmic individuals. Further, nasal mucosa of 4 postmortem individuals was investigated. Immunohistochemical examinations were performed with antibodies against olfactory marker protein (OMP), protein gene product 9.5 (PGP 9.5), beta‐tubulin, (BT), proliferation‐associated antigen (Ki 67), the stem cell marker nestin, cytokeratin, p75NGFr, and α‐synuclein. Most of the biopsy specimens exhibited irregular areas of olfactory‐like, dysplastic epithelium positive for either PGP 9.5 or BT, but negative for OMP. No major histochemical differences in either the expression or distribution of these proteins were observed in the olfactory epithelium of patients with PD compared with controls. Reverse transcription PCR (RT‐PCR) data indicated mRNA for OMP in almost all subjects, independently of their olfactory performance. These data support the idea that olfactory loss in Parkinson's disease is not a consequence of damage to the olfactory epithelium but rather results from distinct central‐nervous abnormalities. © 2009 Movement Disorder Society</abstract><qualityIndicators><score>7.565</score><pdfVersion>1.3</pdfVersion><pdfPageSize>612 x 810 pts</pdfPageSize><refBibsNative>true</refBibsNative><abstractCharCount>1738</abstractCharCount><pdfWordCount>4577</pdfWordCount><pdfCharCount>29786</pdfCharCount><pdfPageCount>9</pdfPageCount><abstractWordCount>249</abstractWordCount></qualityIndicators><title>Biopsies of olfactory epithelium in patients with Parkinson's disease</title><genre><json:string>Serial article</json:string></genre><host><volume>24</volume><pages><total>9</total><last>914</last><first>906</first></pages><issn><json:string>0885-3185</json:string></issn><issue>6</issue><subject><json:item><value>Research Article</value></json:item></subject><genre></genre><language><json:string>unknown</json:string></language><title>Movement Disorders</title><doi><json:string>10.1002/(ISSN)1531-8257</json:string></doi></host><publicationDate>2009</publicationDate><copyrightDate>2009</copyrightDate><doi><json:string>10.1002/mds.22464</json:string></doi><id>0744835A9C2F9F6258CD0268AD01409B7A6B4805</id><fulltext><json:item><original>true</original><mimetype>application/pdf</mimetype><extension>pdf</extension><uri>https://api.istex.fr/document/0744835A9C2F9F6258CD0268AD01409B7A6B4805/fulltext/pdf</uri></json:item><json:item><original>false</original><mimetype>application/zip</mimetype><extension>zip</extension><uri>https://api.istex.fr/document/0744835A9C2F9F6258CD0268AD01409B7A6B4805/fulltext/zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/document/0744835A9C2F9F6258CD0268AD01409B7A6B4805/fulltext/tei"><teiHeader type="text"><fileDesc><titleStmt><title level="a" type="main" xml:lang="en">Biopsies of olfactory epithelium in patients with Parkinson's disease</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><pubPlace>Hoboken</pubPlace><availability><p>Wiley Subscription Services, Inc., A Wiley Company</p></availability><date>2009</date></publicationStmt><notesStmt><note type="content">*Potential conflict of interest: Nothing to report.</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a" type="main" xml:lang="en">Biopsies of olfactory epithelium in patients with Parkinson's disease</title><author><persName><forename type="first">Martin</forename><surname>Witt</surname><roleName type="degree">MD</roleName></persName><note type="correspondence"><p>Correspondence: Department of Anatomy, University of Rostock, Gertrudenstr. 9, 18057 Rostock, Germany</p></note><affiliation>Department of Anatomy, University of Rostock, 18057 Rostock, Germany</affiliation><affiliation>Department of Otorhinolaryngology, Interdisciplinary Center for Smell and Taste, University of Dresden Medical School, 01307 Dresden, Germany</affiliation></author><author><persName><forename type="first">Katja</forename><surname>Bormann</surname></persName><affiliation>Department of Anatomy, University of Dresden Medical School, 01307 Dresden, Germany</affiliation></author><author><persName><forename type="first">Volker</forename><surname>Gudziol</surname><roleName type="degree">MD</roleName></persName><affiliation>Department of Otorhinolaryngology, Interdisciplinary Center for Smell and Taste, University of Dresden Medical School, 01307 Dresden, Germany</affiliation></author><author><persName><forename type="first">Kerstin</forename><surname>Pehlke</surname></persName><affiliation>Department of Anatomy, University of Dresden Medical School, 01307 Dresden, Germany</affiliation></author><author><persName><forename type="first">Kathrin</forename><surname>Barth</surname><roleName type="degree">PhD</roleName></persName><affiliation>Department of Anatomy, University of Dresden Medical School, 01307 Dresden, Germany</affiliation></author><author><persName><forename type="first">Amir</forename><surname>Minovi</surname><roleName type="degree">MD</roleName></persName><affiliation>Department of Otorhinolaryngology, University of Bochum Medical School, 44787 Bochum, Germany</affiliation></author><author><persName><forename type="first">Antje</forename><surname>Hähner</surname><roleName type="degree">MD</roleName></persName><affiliation>Department of Otorhinolaryngology, Interdisciplinary Center for Smell and Taste, University of Dresden Medical School, 01307 Dresden, Germany</affiliation><affiliation>Department of Neurology, University of Dresden Medical School, 01307 Dresden, Germany</affiliation></author><author><persName><forename type="first">Heinz</forename><surname>Reichmann</surname><roleName type="degree">MD</roleName></persName><affiliation>Department of Neurology, University of Dresden Medical School, 01307 Dresden, Germany</affiliation></author><author><persName><forename type="first">Thomas</forename><surname>Hummel</surname><roleName type="degree">MD</roleName></persName><affiliation>Department of Otorhinolaryngology, Interdisciplinary Center for Smell and Taste, University of Dresden Medical School, 01307 Dresden, Germany</affiliation></author></analytic><monogr><title level="j">Movement Disorders</title><title level="j" type="sub">Official Journal of the Movement Disorder Society</title><title level="j" type="abbrev">Mov. Disord.</title><idno type="pISSN">0885-3185</idno><idno type="eISSN">1531-8257</idno><idno type="DOI">10.1002/(ISSN)1531-8257</idno><imprint><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><pubPlace>Hoboken</pubPlace><date type="published" when="2009-04-30"></date><biblScope unit="vol">24</biblScope><biblScope unit="issue">6</biblScope><biblScope unit="page" from="906">906</biblScope><biblScope unit="page" to="914">914</biblScope></imprint></monogr><idno type="istex">0744835A9C2F9F6258CD0268AD01409B7A6B4805</idno><idno type="DOI">10.1002/mds.22464</idno><idno type="ArticleID">MDS22464</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>2009</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Parkinson's disease (PD) is a neurodegenerative disorder involving several neuronal systems. Impaired olfactory function may constitute one of the earliest symptoms of PD. However, it is still unclear to what degree changes of the olfactory epithelium may contribute to dysosmia and if these changes are different from those of other hyposmic or anosmic patients. This study aimed to investigate the hypothesis that olfactory loss in PD is a consequence of specific PD‐related damage of olfactory epithelium. Biopsies of 7 patients diagnosed with PD were taken. Six patients with PD were hyposmic, one anosmic. As non‐PD controls served 9 patients with hyposmia, 9 with anosmia, and 7 normosmic individuals. Further, nasal mucosa of 4 postmortem individuals was investigated. Immunohistochemical examinations were performed with antibodies against olfactory marker protein (OMP), protein gene product 9.5 (PGP 9.5), beta‐tubulin, (BT), proliferation‐associated antigen (Ki 67), the stem cell marker nestin, cytokeratin, p75NGFr, and α‐synuclein. Most of the biopsy specimens exhibited irregular areas of olfactory‐like, dysplastic epithelium positive for either PGP 9.5 or BT, but negative for OMP. No major histochemical differences in either the expression or distribution of these proteins were observed in the olfactory epithelium of patients with PD compared with controls. Reverse transcription PCR (RT‐PCR) data indicated mRNA for OMP in almost all subjects, independently of their olfactory performance. These data support the idea that olfactory loss in Parkinson's disease is not a consequence of damage to the olfactory epithelium but rather results from distinct central‐nervous abnormalities. © 2009 Movement Disorder Society</p></abstract><textClass xml:lang="en"><keywords scheme="keyword"><list><head>Keywords</head><item><term>anosmia</term></item><item><term>hyposmia</term></item><item><term>smell</term></item><item><term>olfactory epithelium</term></item><item><term>immunohistochemistry</term></item><item><term>RT‐PCR</term></item></list></keywords></textClass><textClass><keywords scheme="Journal Subject"><list><head>Article category</head><item><term>Research Article</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="2008-11-14">Received</change><change when="2008-12-24">Registration</change><change when="2009-04-30">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><original>false</original><mimetype>text/plain</mimetype><extension>txt</extension><uri>https://api.istex.fr/document/0744835A9C2F9F6258CD0268AD01409B7A6B4805/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Wiley, elements deleted: body"><istex:xmlDeclaration>version="1.0" encoding="UTF-8" standalone="yes"</istex:xmlDeclaration><istex:document><component version="2.0" type="serialArticle" xml:lang="en"><header><publicationMeta level="product"><publisherInfo><publisherName>Wiley Subscription Services, Inc., A Wiley Company</publisherName><publisherLoc>Hoboken</publisherLoc></publisherInfo><doi registered="yes">10.1002/(ISSN)1531-8257</doi><issn type="print">0885-3185</issn><issn type="electronic">1531-8257</issn><idGroup><id type="product" value="MDS"></id></idGroup><titleGroup><title type="main" xml:lang="en" sort="MOVEMENT DISORDERS">Movement Disorders</title><title type="subtitle">Official Journal of the Movement Disorder Society</title><title type="short">Mov. Disord.</title></titleGroup></publicationMeta><publicationMeta level="part" position="60"><doi origin="wiley" registered="yes">10.1002/mds.v24:6</doi><numberingGroup><numbering type="journalVolume" number="24">24</numbering><numbering type="journalIssue">6</numbering></numberingGroup><coverDate startDate="2009-04-30">30 April 2009</coverDate></publicationMeta><publicationMeta level="unit" type="article" position="160" status="forIssue"><doi origin="wiley" registered="yes">10.1002/mds.22464</doi><idGroup><id type="unit" value="MDS22464"></id></idGroup><countGroup><count type="pageTotal" number="9"></count></countGroup><titleGroup><title type="articleCategory">Research Article</title><title type="tocHeading1">Research Articles</title></titleGroup><copyright ownership="thirdParty">Copyright © 2009 Movement Disorder Society</copyright><eventGroup><event type="manuscriptReceived" date="2008-11-14"></event><event type="manuscriptRevised" date="2008-12-17"></event><event type="manuscriptAccepted" date="2008-12-24"></event><event type="publishedOnlineEarlyUnpaginated" date="2009-02-09"></event><event type="firstOnline" date="2009-02-09"></event><event type="publishedOnlineFinalForm" date="2009-04-24"></event><event type="xmlConverted" agent="Converter:JWSART34_TO_WML3G version:2.3.6 mode:FullText source:FullText result:FullText" date="2010-04-20"></event><event type="xmlConverted" agent="Converter:WILEY_ML3G_TO_WILEY_ML3GV2 version:3.8.8" date="2014-02-02"></event><event type="xmlConverted" agent="Converter:WML3G_To_WML3G version:4.1.7 mode:FullText,remove_FC" date="2014-10-31"></event></eventGroup><numberingGroup><numbering type="pageFirst">906</numbering><numbering type="pageLast">914</numbering></numberingGroup><correspondenceTo>Department of Anatomy, University of Rostock, Gertrudenstr. 9, 18057 Rostock, Germany</correspondenceTo><linkGroup><link type="toTypesetVersion" href="file:MDS.MDS22464.pdf"></link></linkGroup></publicationMeta><contentMeta><countGroup><count type="figureTotal" number="4"></count><count type="tableTotal" number="0"></count><count type="referenceTotal" number="40"></count><count type="wordTotal" number="4982"></count></countGroup><titleGroup><title type="main" xml:lang="en">Biopsies of olfactory epithelium in patients with Parkinson's disease<link href="#fn1"></link></title><title type="short" xml:lang="en">Olfactory Biopsies in Parkinson Disease</title></titleGroup><creators><creator xml:id="au1" creatorRole="author" affiliationRef="#af1 #af2" corresponding="yes"><personName><givenNames>Martin</givenNames><familyName>Witt</familyName><degrees>MD</degrees></personName><contactDetails><email normalForm="martin.witt@med.uni-rostock.de">martin.witt@med.uni‐rostock.de</email></contactDetails></creator><creator xml:id="au2" creatorRole="author" affiliationRef="#af3"><personName><givenNames>Katja</givenNames><familyName>Bormann</familyName></personName></creator><creator xml:id="au3" creatorRole="author" affiliationRef="#af2"><personName><givenNames>Volker</givenNames><familyName>Gudziol</familyName><degrees>MD</degrees></personName></creator><creator xml:id="au4" creatorRole="author" affiliationRef="#af3"><personName><givenNames>Kerstin</givenNames><familyName>Pehlke</familyName></personName></creator><creator xml:id="au5" creatorRole="author" affiliationRef="#af3"><personName><givenNames>Kathrin</givenNames><familyName>Barth</familyName><degrees>PhD</degrees></personName></creator><creator xml:id="au6" creatorRole="author" affiliationRef="#af4"><personName><givenNames>Amir</givenNames><familyName>Minovi</familyName><degrees>MD</degrees></personName></creator><creator xml:id="au7" creatorRole="author" affiliationRef="#af2 #af5"><personName><givenNames>Antje</givenNames><familyName>Hähner</familyName><degrees>MD</degrees></personName></creator><creator xml:id="au8" creatorRole="author" affiliationRef="#af5"><personName><givenNames>Heinz</givenNames><familyName>Reichmann</familyName><degrees>MD</degrees></personName></creator><creator xml:id="au9" creatorRole="author" affiliationRef="#af2"><personName><givenNames>Thomas</givenNames><familyName>Hummel</familyName><degrees>MD</degrees></personName></creator></creators><affiliationGroup><affiliation xml:id="af1" countryCode="DE" type="organization"><unparsedAffiliation>Department of Anatomy, University of Rostock, 18057 Rostock, Germany</unparsedAffiliation></affiliation><affiliation xml:id="af2" countryCode="DE" type="organization"><unparsedAffiliation>Department of Otorhinolaryngology, Interdisciplinary Center for Smell and Taste, University of Dresden Medical School, 01307 Dresden, Germany</unparsedAffiliation></affiliation><affiliation xml:id="af3" countryCode="DE" type="organization"><unparsedAffiliation>Department of Anatomy, University of Dresden Medical School, 01307 Dresden, Germany</unparsedAffiliation></affiliation><affiliation xml:id="af4" countryCode="DE" type="organization"><unparsedAffiliation>Department of Otorhinolaryngology, University of Bochum Medical School, 44787 Bochum, Germany</unparsedAffiliation></affiliation><affiliation xml:id="af5" countryCode="DE" type="organization"><unparsedAffiliation>Department of Neurology, University of Dresden Medical School, 01307 Dresden, Germany</unparsedAffiliation></affiliation></affiliationGroup><keywordGroup xml:lang="en" type="author"><keyword xml:id="kwd1">anosmia</keyword><keyword xml:id="kwd2">hyposmia</keyword><keyword xml:id="kwd3">smell</keyword><keyword xml:id="kwd4">olfactory epithelium</keyword><keyword xml:id="kwd5">immunohistochemistry</keyword><keyword xml:id="kwd6">RT‐PCR</keyword></keywordGroup><supportingInformation><p> Additional Supporting Information may be found in the online version of this article. </p><supportingInfoItem><mediaResource alt="supporting information" href="urn-x:wiley:08853185:media:mds22464:MDS_22464_sm_suppinfo"></mediaResource><caption>Supplemental Methods</caption></supportingInfoItem><supportingInfoItem><mediaResource alt="supporting information" href="urn-x:wiley:08853185:media:mds22464:MDS_22464_sm_supptable1"></mediaResource><caption>Supporting Table 1. Survey of the participants of this study. The data in the “type” column refer to classification of olfactory epithelium (OE) in at least one of the samples, as defined in Supplemental Table 2. PD, Parkinson's disease.</caption></supportingInfoItem><supportingInfoItem><mediaResource alt="supporting information" href="urn-x:wiley:08853185:media:mds22464:MDS_22464_sm_supptable2"></mediaResource><caption>Supporting Table 2. Classification of olfactory epithelium.</caption></supportingInfoItem><supportingInfoItem><mediaResource alt="supporting information" href="urn-x:wiley:08853185:media:mds22464:MDS_22464_sm_supptable3"></mediaResource><caption>Supporting Table 3. Oligonucleotid sequences of primers used for RT‐PCR.</caption></supportingInfoItem><supportingInfoItem><mediaResource alt="supporting information" href="urn-x:wiley:08853185:media:mds22464:MDS_22464_sm_suppfig1"></mediaResource><caption>Supporting Figure 1. Schematic view of the lateral wall of the nasal cavity. Biopsies were taken from the insertion of the medial turbinate (arrows, see also “MT” in Figure 2) and from the dorsal septum at the same level (not shown).</caption></supportingInfoItem><supportingInfoItem><mediaResource alt="supporting information" href="urn-x:wiley:08853185:media:mds22464:MDS_22464_sm_suppfig2"></mediaResource><caption>Supporting Figure 2. Immunohistochemical detection of α‐synuclein in the olfactory mucosa of two patients with Parkinson's disease (A, #263; B, #264). There is no reactivity of α‐synuclein. No counterstain in A(, haematoxylin counterstain in B. Scale bar: 50 μm.</caption></supportingInfoItem><supportingInfoItem><mediaResource alt="supporting information" href="urn-x:wiley:08853185:media:mds22464:MDS_22464_sm_suppfig3"></mediaResource><caption>Supporting Figure 3. A: RT‐PCR amplification of OMP mRNA in selected individuals: hyposmic patients with Parkinson's disease (265‐P, 274‐P), hyposmic (284, 276), anosmic (280, 273) and normosmic (278, 279) patients. Most of them showed a positive band for gene products, except patient #276. Even normosmic individuals presented relatively weak OMP bands. Patient #265‐P (PD) revealed 3 positive OMP bands in different biopsy sites. B This survey illustrates the occurrence of mRNAs for several gene products associated with neurogenetic processes. mRNAs for P75NGFr, nestin, and beta‐tubulin are expressed in all subjects investigated, regardless of their olfactory status.</caption></supportingInfoItem></supportingInformation><abstractGroup><abstract type="main" xml:lang="en"><title type="main">Abstract</title><p>Parkinson's disease (PD) is a neurodegenerative disorder involving several neuronal systems. Impaired olfactory function may constitute one of the earliest symptoms of PD. However, it is still unclear to what degree changes of the olfactory epithelium may contribute to dysosmia and if these changes are different from those of other hyposmic or anosmic patients. This study aimed to investigate the hypothesis that olfactory loss in PD is a consequence of specific PD‐related damage of olfactory epithelium. Biopsies of 7 patients diagnosed with PD were taken. Six patients with PD were hyposmic, one anosmic. As non‐PD controls served 9 patients with hyposmia, 9 with anosmia, and 7 normosmic individuals. Further, nasal mucosa of 4 postmortem individuals was investigated. Immunohistochemical examinations were performed with antibodies against olfactory marker protein (OMP), protein gene product 9.5 (PGP 9.5), beta‐tubulin, (BT), proliferation‐associated antigen (Ki 67), the stem cell marker nestin, cytokeratin, p75NGFr, and α‐synuclein. Most of the biopsy specimens exhibited irregular areas of olfactory‐like, dysplastic epithelium positive for either PGP 9.5 or BT, but negative for OMP. No major histochemical differences in either the expression or distribution of these proteins were observed in the olfactory epithelium of patients with PD compared with controls. Reverse transcription PCR (RT‐PCR) data indicated mRNA for OMP in almost all subjects, independently of their olfactory performance. These data support the idea that olfactory loss in Parkinson's disease is not a consequence of damage to the olfactory epithelium but rather results from distinct central‐nervous abnormalities. © 2009 Movement Disorder Society</p></abstract></abstractGroup></contentMeta><noteGroup><note xml:id="fn1"><p>Potential conflict of interest: Nothing to report.</p></note></noteGroup></header></component></istex:document></istex:metadataXml><!--Version 0.6 générée le 4-12-2015--><mods version="3.6"><titleInfo lang="en"><title>Biopsies of olfactory epithelium in patients with Parkinson's disease</title></titleInfo><titleInfo type="abbreviated" lang="en"><title>Olfactory Biopsies in Parkinson Disease</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Biopsies of olfactory epithelium in patients with Parkinson's disease</title></titleInfo><name type="personal"><namePart type="given">Martin</namePart><namePart type="family">Witt</namePart><namePart type="termsOfAddress">MD</namePart><affiliation>Department of Anatomy, University of Rostock, 18057 Rostock, Germany</affiliation><affiliation>Department of Otorhinolaryngology, Interdisciplinary Center for Smell and Taste, University of Dresden Medical School, 01307 Dresden, Germany</affiliation><description>Correspondence: Department of Anatomy, University of Rostock, Gertrudenstr. 9, 18057 Rostock, Germany</description><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Katja</namePart><namePart type="family">Bormann</namePart><affiliation>Department of Anatomy, University of Dresden Medical School, 01307 Dresden, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Volker</namePart><namePart type="family">Gudziol</namePart><namePart type="termsOfAddress">MD</namePart><affiliation>Department of Otorhinolaryngology, Interdisciplinary Center for Smell and Taste, University of Dresden Medical School, 01307 Dresden, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Kerstin</namePart><namePart type="family">Pehlke</namePart><affiliation>Department of Anatomy, University of Dresden Medical School, 01307 Dresden, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Kathrin</namePart><namePart type="family">Barth</namePart><namePart type="termsOfAddress">PhD</namePart><affiliation>Department of Anatomy, University of Dresden Medical School, 01307 Dresden, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Amir</namePart><namePart type="family">Minovi</namePart><namePart type="termsOfAddress">MD</namePart><affiliation>Department of Otorhinolaryngology, University of Bochum Medical School, 44787 Bochum, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Antje</namePart><namePart type="family">Hähner</namePart><namePart type="termsOfAddress">MD</namePart><affiliation>Department of Otorhinolaryngology, Interdisciplinary Center for Smell and Taste, University of Dresden Medical School, 01307 Dresden, Germany</affiliation><affiliation>Department of Neurology, University of Dresden Medical School, 01307 Dresden, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Heinz</namePart><namePart type="family">Reichmann</namePart><namePart type="termsOfAddress">MD</namePart><affiliation>Department of Neurology, University of Dresden Medical School, 01307 Dresden, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Thomas</namePart><namePart type="family">Hummel</namePart><namePart type="termsOfAddress">MD</namePart><affiliation>Department of Otorhinolaryngology, Interdisciplinary Center for Smell and Taste, University of Dresden Medical School, 01307 Dresden, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre authority="originalCategForm">article</genre><originInfo><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><place><placeTerm type="text">Hoboken</placeTerm></place><dateIssued encoding="w3cdtf">2009-04-30</dateIssued><dateCaptured encoding="w3cdtf">2008-11-14</dateCaptured><dateValid encoding="w3cdtf">2008-12-24</dateValid><copyrightDate encoding="w3cdtf">2009</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType><extent unit="figures">4</extent><extent unit="references">40</extent><extent unit="words">4982</extent></physicalDescription><abstract lang="en">Parkinson's disease (PD) is a neurodegenerative disorder involving several neuronal systems. Impaired olfactory function may constitute one of the earliest symptoms of PD. However, it is still unclear to what degree changes of the olfactory epithelium may contribute to dysosmia and if these changes are different from those of other hyposmic or anosmic patients. This study aimed to investigate the hypothesis that olfactory loss in PD is a consequence of specific PD‐related damage of olfactory epithelium. Biopsies of 7 patients diagnosed with PD were taken. Six patients with PD were hyposmic, one anosmic. As non‐PD controls served 9 patients with hyposmia, 9 with anosmia, and 7 normosmic individuals. Further, nasal mucosa of 4 postmortem individuals was investigated. Immunohistochemical examinations were performed with antibodies against olfactory marker protein (OMP), protein gene product 9.5 (PGP 9.5), beta‐tubulin, (BT), proliferation‐associated antigen (Ki 67), the stem cell marker nestin, cytokeratin, p75NGFr, and α‐synuclein. Most of the biopsy specimens exhibited irregular areas of olfactory‐like, dysplastic epithelium positive for either PGP 9.5 or BT, but negative for OMP. No major histochemical differences in either the expression or distribution of these proteins were observed in the olfactory epithelium of patients with PD compared with controls. Reverse transcription PCR (RT‐PCR) data indicated mRNA for OMP in almost all subjects, independently of their olfactory performance. These data support the idea that olfactory loss in Parkinson's disease is not a consequence of damage to the olfactory epithelium but rather results from distinct central‐nervous abnormalities. © 2009 Movement Disorder Society</abstract><note type="content">*Potential conflict of interest: Nothing to report.</note><subject lang="en"><genre>Keywords</genre><topic>anosmia</topic><topic>hyposmia</topic><topic>smell</topic><topic>olfactory epithelium</topic><topic>immunohistochemistry</topic><topic>RT‐PCR</topic></subject><relatedItem type="host"><titleInfo><title>Movement Disorders</title><subTitle>Official Journal of the Movement Disorder Society</subTitle></titleInfo><titleInfo type="abbreviated"><title>Mov. Disord.</title></titleInfo><note type="content"> Additional Supporting Information may be found in the online version of this article.Supporting Info Item: Supplemental Methods - Supporting Table 1. Survey of the participants of this study. The data in the “type” column refer to classification of olfactory epithelium (OE) in at least one of the samples, as defined in Supplemental Table 2. PD, Parkinson's disease. - Supporting Table 2. Classification of olfactory epithelium. - Supporting Table 3. Oligonucleotid sequences of primers used for RT‐PCR. - Supporting Figure 1. Schematic view of the lateral wall of the nasal cavity. Biopsies were taken from the insertion of the medial turbinate (arrows, see also “MT” in Figure 2) and from the dorsal septum at the same level (not shown). - Supporting Figure 2. Immunohistochemical detection of α‐synuclein in the olfactory mucosa of two patients with Parkinson's disease (A, #263; B, #264). There is no reactivity of α‐synuclein. No counterstain in A(, haematoxylin counterstain in B. Scale bar: 50 μm. - Supporting Figure 3. A: RT‐PCR amplification of OMP mRNA in selected individuals: hyposmic patients with Parkinson's disease (265‐P, 274‐P), hyposmic (284, 276), anosmic (280, 273) and normosmic (278, 279) patients. Most of them showed a positive band for gene products, except patient #276. Even normosmic individuals presented relatively weak OMP bands. Patient #265‐P (PD) revealed 3 positive OMP bands in different biopsy sites. B This survey illustrates the occurrence of mRNAs for several gene products associated with neurogenetic processes. mRNAs for P75NGFr, nestin, and beta‐tubulin are expressed in all subjects investigated, regardless of their olfactory status. - </note><subject><genre>article category</genre><topic>Research Article</topic></subject><identifier type="ISSN">0885-3185</identifier><identifier type="eISSN">1531-8257</identifier><identifier type="DOI">10.1002/(ISSN)1531-8257</identifier><identifier type="PublisherID">MDS</identifier><part><date>2009</date><detail type="volume"><caption>vol.</caption><number>24</number></detail><detail type="issue"><caption>no.</caption><number>6</number></detail><extent unit="pages"><start>906</start><end>914</end><total>9</total></extent></part></relatedItem><identifier type="istex">0744835A9C2F9F6258CD0268AD01409B7A6B4805</identifier><identifier type="DOI">10.1002/mds.22464</identifier><identifier type="ArticleID">MDS22464</identifier><accessCondition type="use and reproduction" contentType="copyright">Copyright © 2009 Movement Disorder Society</accessCondition><recordInfo><recordOrigin>Wiley Subscription Services, Inc., A Wiley Company</recordOrigin><recordContentSource>WILEY</recordContentSource></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Santé/explor/MovDisordV3/Data/Istex/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000497 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Istex/Corpus/biblio.hfd -nk 000497 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Santé
|area= MovDisordV3
|flux= Istex
|étape= Corpus
|type= RBID
|clé= ISTEX:0744835A9C2F9F6258CD0268AD01409B7A6B4805
|texte= Biopsies of olfactory epithelium in patients with Parkinson's disease
}}
| This area was generated with Dilib version V0.6.23. |  |