In vitro regeneration of loblolly pine and random amplified polymorphic DNA analyses of regenerated plantlets.
Identifieur interne : 002567 ( PubMed/Curation ); précédent : 002566; suivant : 002568In vitro regeneration of loblolly pine and random amplified polymorphic DNA analyses of regenerated plantlets.
Auteurs : W. TangSource :
- Plant cell reports [ 1432-203X ] ; 2001.
Abstract
Adventitious buds were induced from organogenic callus derived from mature zygotic embryos of three lines (E-311, E-440, and E-822) of loblolly pine (Pinus taeda L.) within 27 weeks of culture. The influence of cytokinins, silver nitrate, and low-temperature treatment on the differentiation of adventitious buds was analyzed. Elongation of adventitious buds was achieved on TE medium supplemented with 0.5 mg/l indole-3-butyric acid (IBA) and 1 mg/l 6-benzyladenine (BA). After adventitious shoots had rooted on TE medium supplemented with 0.5 mg/l IBA, 2 mg/l BA, and 0.5 mg/l gibberellic acid, 498 regenerated plantlets were transferred to a perlite:peatmoss:vermiculite (1 :>: 1) soil mixture; 351 of these survived in the field. Total DNA was extracted from 21 regenerated plantlets randomly chosen from the 151 regenerated plantlets of line E-822. Random amplified polymorphic DNA (RAPD) analysis using 80 arbitrary oligonucleotide 10-mers showed that 21 primers gave 107 clear reproducible bands, with the amplification products being monomorphic for all of the plantlets of line E-822 tested. A total of 2,247 bands obtained from these studies exhibited no aberration in RAPD banding patterns among the tested plantlets. These results suggest that somatic organogenesis can be used for clonal micropropagation of some lines of loblolly pine without the fear of the appearance of unwanted somaclonal variants.
DOI: 10.1007/s002990000297
PubMed: 30759904
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W. Tang<affiliation><nlm:affiliation>State Key Laboratory of Biochemical Engineering, Institute of Chemical Metallurgy, Chinese Academy of Sciences, Beijing 100080, P. R. China e-mail: wtang@unity.ncsu.edu Fax: +1-919-5157801, , , , , , CN.</nlm:affiliation><wicri:noCountry code="subField">CN</wicri:noCountry></affiliation>Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">In vitro regeneration of loblolly pine and random amplified polymorphic DNA analyses of regenerated plantlets.</title><author><name sortKey="Tang, W" sort="Tang, W" uniqKey="Tang W" first="W" last="Tang">W. Tang</name><affiliation><nlm:affiliation>State Key Laboratory of Biochemical Engineering, Institute of Chemical Metallurgy, Chinese Academy of Sciences, Beijing 100080, P. R. China e-mail: wtang@unity.ncsu.edu Fax: +1-919-5157801, , , , , , CN.</nlm:affiliation><wicri:noCountry code="subField">CN</wicri:noCountry></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2001">2001</date><idno type="RBID">pubmed:30759904</idno><idno type="pmid">30759904</idno><idno type="doi">10.1007/s002990000297</idno><idno type="wicri:Area/PubMed/Corpus">002567</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002567</idno><idno type="wicri:Area/PubMed/Curation">002567</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002567</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">In vitro regeneration of loblolly pine and random amplified polymorphic DNA analyses of regenerated plantlets.</title><author><name sortKey="Tang, W" sort="Tang, W" uniqKey="Tang W" first="W" last="Tang">W. Tang</name><affiliation><nlm:affiliation>State Key Laboratory of Biochemical Engineering, Institute of Chemical Metallurgy, Chinese Academy of Sciences, Beijing 100080, P. R. China e-mail: wtang@unity.ncsu.edu Fax: +1-919-5157801, , , , , , CN.</nlm:affiliation><wicri:noCountry code="subField">CN</wicri:noCountry></affiliation></author></analytic><series><title level="j">Plant cell reports</title><idno type="eISSN">1432-203X</idno><imprint><date when="2001" type="published">2001</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"> Adventitious buds were induced from organogenic callus derived from mature zygotic embryos of three lines (E-311, E-440, and E-822) of loblolly pine (Pinus taeda L.) within 27 weeks of culture. The influence of cytokinins, silver nitrate, and low-temperature treatment on the differentiation of adventitious buds was analyzed. Elongation of adventitious buds was achieved on TE medium supplemented with 0.5 mg/l indole-3-butyric acid (IBA) and 1 mg/l 6-benzyladenine (BA). After adventitious shoots had rooted on TE medium supplemented with 0.5 mg/l IBA, 2 mg/l BA, and 0.5 mg/l gibberellic acid, 498 regenerated plantlets were transferred to a perlite:peatmoss:vermiculite (1 :>: 1) soil mixture; 351 of these survived in the field. Total DNA was extracted from 21 regenerated plantlets randomly chosen from the 151 regenerated plantlets of line E-822. Random amplified polymorphic DNA (RAPD) analysis using 80 arbitrary oligonucleotide 10-mers showed that 21 primers gave 107 clear reproducible bands, with the amplification products being monomorphic for all of the plantlets of line E-822 tested. A total of 2,247 bands obtained from these studies exhibited no aberration in RAPD banding patterns among the tested plantlets. These results suggest that somatic organogenesis can be used for clonal micropropagation of some lines of loblolly pine without the fear of the appearance of unwanted somaclonal variants.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">30759904</PMID><DateRevised><Year>2019</Year><Month>11</Month><Day>20</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1432-203X</ISSN><JournalIssue CitedMedium="Internet"><Volume>20</Volume><Issue>2</Issue><PubDate><Year>2001</Year><Month>Feb</Month></PubDate></JournalIssue><Title>Plant cell reports</Title><ISOAbbreviation>Plant Cell Rep.</ISOAbbreviation></Journal><ArticleTitle>In vitro regeneration of loblolly pine and random amplified polymorphic DNA analyses of regenerated plantlets.</ArticleTitle><Pagination><MedlinePgn>163-168</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s002990000297</ELocationID><Abstract><AbstractText> Adventitious buds were induced from organogenic callus derived from mature zygotic embryos of three lines (E-311, E-440, and E-822) of loblolly pine (Pinus taeda L.) within 27 weeks of culture. The influence of cytokinins, silver nitrate, and low-temperature treatment on the differentiation of adventitious buds was analyzed. Elongation of adventitious buds was achieved on TE medium supplemented with 0.5 mg/l indole-3-butyric acid (IBA) and 1 mg/l 6-benzyladenine (BA). After adventitious shoots had rooted on TE medium supplemented with 0.5 mg/l IBA, 2 mg/l BA, and 0.5 mg/l gibberellic acid, 498 regenerated plantlets were transferred to a perlite:peatmoss:vermiculite (1 :>: 1) soil mixture; 351 of these survived in the field. Total DNA was extracted from 21 regenerated plantlets randomly chosen from the 151 regenerated plantlets of line E-822. Random amplified polymorphic DNA (RAPD) analysis using 80 arbitrary oligonucleotide 10-mers showed that 21 primers gave 107 clear reproducible bands, with the amplification products being monomorphic for all of the plantlets of line E-822 tested. A total of 2,247 bands obtained from these studies exhibited no aberration in RAPD banding patterns among the tested plantlets. These results suggest that somatic organogenesis can be used for clonal micropropagation of some lines of loblolly pine without the fear of the appearance of unwanted somaclonal variants.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Tang</LastName><ForeName>W</ForeName><Initials>W</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Biochemical Engineering, Institute of Chemical Metallurgy, Chinese Academy of Sciences, Beijing 100080, P. R. China e-mail: wtang@unity.ncsu.edu Fax: +1-919-5157801, , , , , , CN.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Plant Cell Rep</MedlineTA><NlmUniqueID>9880970</NlmUniqueID><ISSNLinking>0721-7714</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">KeywordsPinus taeda L.</Keyword><Keyword MajorTopicYN="N">Organogenesis</Keyword><Keyword MajorTopicYN="N">Plant regeneration</Keyword><Keyword MajorTopicYN="N">Random amplified polymorphic DNA</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2019</Year><Month>2</Month><Day>15</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2001</Year><Month>2</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2001</Year><Month>2</Month><Day>1</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">30759904</ArticleId><ArticleId IdType="doi">10.1007/s002990000297</ArticleId><ArticleId IdType="pii">10.1007/s002990000297</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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