Computer-based design of an HLA-haplotype and HIV-clade independent cytotoxic T-lymphocyte assay for monitoring HIV-specific immunity.
Identifieur interne : 002469 ( PubMed/Curation ); précédent : 002468; suivant : 002470Computer-based design of an HLA-haplotype and HIV-clade independent cytotoxic T-lymphocyte assay for monitoring HIV-specific immunity.
Auteurs : Massimo Amicosante [Italie] ; Cristiana Gioia ; Carla Montesano ; Rita Casetti ; Simone Topino ; Gianpiero D'Offizi ; Giulia Cappelli ; Giuseppe Ippolito ; Vittorio Colizzi ; Fabrizio Poccia ; Leopoldo P. PucilloSource :
- Molecular medicine (Cambridge, Mass.) [ 1076-1551 ] ; 2002.
Descripteurs français
- KwdFr :
- Adulte, Adulte d'âge moyen, Antigènes HLA (immunologie), Données de séquences moléculaires, Dosage biologique, Femelle, Humains, Interféron gamma (métabolisme), Lymphocytes T cytotoxiques (immunologie), Lymphocytes T cytotoxiques (métabolisme), Mâle, Peptides (immunologie), Produits du gène gag (immunologie), Séquence d'acides aminés, VIH (Virus de l'Immunodéficience Humaine) (immunologie).
- MESH :
- immunologie : Antigènes HLA, Lymphocytes T cytotoxiques, Peptides, Produits du gène gag, VIH (Virus de l'Immunodéficience Humaine).
- métabolisme : Interféron gamma, Lymphocytes T cytotoxiques.
- Adulte, Adulte d'âge moyen, Données de séquences moléculaires, Dosage biologique, Femelle, Humains, Mâle, Séquence d'acides aminés.
English descriptors
- KwdEn :
- Adult, Amino Acid Sequence, Biological Assay, Female, Gene Products, gag (immunology), HIV (immunology), HLA Antigens (immunology), Humans, Interferon-gamma (metabolism), Male, Middle Aged, Molecular Sequence Data, Peptides (immunology), T-Lymphocytes, Cytotoxic (immunology), T-Lymphocytes, Cytotoxic (metabolism).
- MESH :
- chemical , immunology : Gene Products, gag, HLA Antigens, Peptides.
- immunology : HIV, T-Lymphocytes, Cytotoxic.
- chemical , metabolism : Interferon-gamma, T-Lymphocytes, Cytotoxic.
- Adult, Amino Acid Sequence, Biological Assay, Female, Humans, Male, Middle Aged, Molecular Sequence Data.
Abstract
Human immunodeficiency virus (HIV)- specific CD8-positive cytotoxic T-lymphocytes (CTL) play a key role in controlling HIV infection. Monitoring CTL response could be clinically relevant during structured therapy interruption (STI), HIV exposure, and vaccine trials. However, HLA patients' restriction and HIV variability limited the development of a CTL assay with broad specificity.
PubMed: 12606814
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Pucillo</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2002">2002</date><idno type="RBID">pubmed:12606814</idno><idno type="pmid">12606814</idno><idno type="wicri:Area/PubMed/Corpus">002469</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002469</idno><idno type="wicri:Area/PubMed/Curation">002469</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002469</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Computer-based design of an HLA-haplotype and HIV-clade independent cytotoxic T-lymphocyte assay for monitoring HIV-specific immunity.</title><author><name sortKey="Amicosante, Massimo" sort="Amicosante, Massimo" uniqKey="Amicosante M" first="Massimo" last="Amicosante">Massimo Amicosante</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Clinical Pathology, National InstiTute for Infectious Disease, Lazzaro Spallanzani, Rome, Italy. amicosante@inmi.it</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Laboratory of Clinical Pathology, National InstiTute for Infectious Disease, Lazzaro Spallanzani, Rome</wicri:regionArea></affiliation></author><author><name sortKey="Gioia, Cristiana" sort="Gioia, Cristiana" uniqKey="Gioia C" first="Cristiana" last="Gioia">Cristiana Gioia</name></author><author><name sortKey="Montesano, Carla" sort="Montesano, Carla" uniqKey="Montesano C" first="Carla" last="Montesano">Carla Montesano</name></author><author><name sortKey="Casetti, Rita" sort="Casetti, Rita" uniqKey="Casetti R" first="Rita" last="Casetti">Rita Casetti</name></author><author><name sortKey="Topino, Simone" sort="Topino, Simone" uniqKey="Topino S" first="Simone" last="Topino">Simone Topino</name></author><author><name sortKey="D Offizi, Gianpiero" sort="D Offizi, Gianpiero" uniqKey="D Offizi G" first="Gianpiero" last="D'Offizi">Gianpiero D'Offizi</name></author><author><name sortKey="Cappelli, Giulia" sort="Cappelli, Giulia" uniqKey="Cappelli G" first="Giulia" last="Cappelli">Giulia Cappelli</name></author><author><name sortKey="Ippolito, Giuseppe" sort="Ippolito, Giuseppe" uniqKey="Ippolito G" first="Giuseppe" last="Ippolito">Giuseppe Ippolito</name></author><author><name sortKey="Colizzi, Vittorio" sort="Colizzi, Vittorio" uniqKey="Colizzi V" first="Vittorio" last="Colizzi">Vittorio Colizzi</name></author><author><name sortKey="Poccia, Fabrizio" sort="Poccia, Fabrizio" uniqKey="Poccia F" first="Fabrizio" last="Poccia">Fabrizio Poccia</name></author><author><name sortKey="Pucillo, Leopoldo P" sort="Pucillo, Leopoldo P" uniqKey="Pucillo L" first="Leopoldo P" last="Pucillo">Leopoldo P. Pucillo</name></author></analytic><series><title level="j">Molecular medicine (Cambridge, Mass.)</title><idno type="ISSN">1076-1551</idno><imprint><date when="2002" type="published">2002</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adult</term><term>Amino Acid Sequence</term><term>Biological Assay</term><term>Female</term><term>Gene Products, gag (immunology)</term><term>HIV (immunology)</term><term>HLA Antigens (immunology)</term><term>Humans</term><term>Interferon-gamma (metabolism)</term><term>Male</term><term>Middle Aged</term><term>Molecular Sequence Data</term><term>Peptides (immunology)</term><term>T-Lymphocytes, Cytotoxic (immunology)</term><term>T-Lymphocytes, Cytotoxic (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Adulte</term><term>Adulte d'âge moyen</term><term>Antigènes HLA (immunologie)</term><term>Données de séquences moléculaires</term><term>Dosage biologique</term><term>Femelle</term><term>Humains</term><term>Interféron gamma (métabolisme)</term><term>Lymphocytes T cytotoxiques (immunologie)</term><term>Lymphocytes T cytotoxiques (métabolisme)</term><term>Mâle</term><term>Peptides (immunologie)</term><term>Produits du gène gag (immunologie)</term><term>Séquence d'acides aminés</term><term>VIH (Virus de l'Immunodéficience Humaine) (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Gene Products, gag</term><term>HLA Antigens</term><term>Peptides</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Antigènes HLA</term><term>Lymphocytes T cytotoxiques</term><term>Peptides</term><term>Produits du gène gag</term><term>VIH (Virus de l'Immunodéficience Humaine)</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>HIV</term><term>T-Lymphocytes, Cytotoxic</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Interferon-gamma</term><term>T-Lymphocytes, Cytotoxic</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Interféron gamma</term><term>Lymphocytes T cytotoxiques</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adult</term><term>Amino Acid Sequence</term><term>Biological Assay</term><term>Female</term><term>Humans</term><term>Male</term><term>Middle Aged</term><term>Molecular Sequence Data</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Adulte</term><term>Adulte d'âge moyen</term><term>Données de séquences moléculaires</term><term>Dosage biologique</term><term>Femelle</term><term>Humains</term><term>Mâle</term><term>Séquence d'acides aminés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Human immunodeficiency virus (HIV)- specific CD8-positive cytotoxic T-lymphocytes (CTL) play a key role in controlling HIV infection. Monitoring CTL response could be clinically relevant during structured therapy interruption (STI), HIV exposure, and vaccine trials. However, HLA patients' restriction and HIV variability limited the development of a CTL assay with broad specificity.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">12606814</PMID><DateCompleted><Year>2003</Year><Month>08</Month><Day>01</Day></DateCompleted><DateRevised><Year>2018</Year><Month>08</Month><Day>21</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">1076-1551</ISSN><JournalIssue CitedMedium="Print"><Volume>8</Volume><Issue>12</Issue><PubDate><Year>2002</Year><Month>Dec</Month></PubDate></JournalIssue><Title>Molecular medicine (Cambridge, Mass.)</Title><ISOAbbreviation>Mol. Med.</ISOAbbreviation></Journal><ArticleTitle>Computer-based design of an HLA-haplotype and HIV-clade independent cytotoxic T-lymphocyte assay for monitoring HIV-specific immunity.</ArticleTitle><Pagination><MedlinePgn>798-807</MedlinePgn></Pagination><Abstract><AbstractText Label="BACKGROUND" NlmCategory="BACKGROUND">Human immunodeficiency virus (HIV)- specific CD8-positive cytotoxic T-lymphocytes (CTL) play a key role in controlling HIV infection. Monitoring CTL response could be clinically relevant during structured therapy interruption (STI), HIV exposure, and vaccine trials. However, HLA patients' restriction and HIV variability limited the development of a CTL assay with broad specificity.</AbstractText><AbstractText Label="MATERIALS AND METHODS" NlmCategory="METHODS">We designed an HLA-class I/HIV-1 clade independent assay for assessing HIV- specific CTL by using a computer-assisted selection ofthe CTL epitopes. Twenty-eight 15-mers were selected by peptide-binding motifs analysis using different databases (HIV-Immunology Database, SYFPEITHI, BIMAS). Altogether they putatively bind to more than 90% of HLA haplotypes in different populations, with an overall HIV-1 variability below 9%. The peptide pool was used as an antigen in an intracellular cytokine staining (ICS) assay for quantifying HIV-specific CTL response.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">The test can be performed using both fresh and cryopreserved peripheral blood mononuclear cells (PBMC), whereas GAG protein as antigen works only on fresh PBMC. A significantly higher CTL response with respect to HIV-negative controls was detected in all HIV-1 infected subjects of two groups of patients with different ethnicities (Caucasians and Africans) and coming from areas with different HIV-1 clade prevalences (clade B and A/G, respectively). In Caucasian patients, after month of STI, the number of HIV-1 specific CTL (2,896 +/- 2,780 IFN-gamma specific CD8 cells/ml) was significantly higher than that found at enrolment (2,125 +/- 4,426 IFN-gamma specific CD8 cells/ml, p< 0.05).</AbstractText><AbstractText Label="CONCLUSIONS" NlmCategory="CONCLUSIONS">These data indicate that this CTL assay is broadly specific and could represent a useful clinical tool for HIV immunodiagnostic independent of HLA-haplotype and HIV-clade variabilities.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Amicosante</LastName><ForeName>Massimo</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Laboratory of Clinical Pathology, National InstiTute for Infectious Disease, Lazzaro Spallanzani, Rome, Italy. amicosante@inmi.it</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gioia</LastName><ForeName>Cristiana</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Montesano</LastName><ForeName>Carla</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Casetti</LastName><ForeName>Rita</ForeName><Initials>R</Initials></Author><Author ValidYN="Y"><LastName>Topino</LastName><ForeName>Simone</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>D'Offizi</LastName><ForeName>Gianpiero</ForeName><Initials>G</Initials></Author><Author ValidYN="Y"><LastName>Cappelli</LastName><ForeName>Giulia</ForeName><Initials>G</Initials></Author><Author ValidYN="Y"><LastName>Ippolito</LastName><ForeName>Giuseppe</ForeName><Initials>G</Initials></Author><Author ValidYN="Y"><LastName>Colizzi</LastName><ForeName>Vittorio</ForeName><Initials>V</Initials></Author><Author ValidYN="Y"><LastName>Poccia</LastName><ForeName>Fabrizio</ForeName><Initials>F</Initials></Author><Author ValidYN="Y"><LastName>Pucillo</LastName><ForeName>Leopoldo P</ForeName><Initials>LP</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Mol Med</MedlineTA><NlmUniqueID>9501023</NlmUniqueID><ISSNLinking>1076-1551</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D015683">Gene Products, gag</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D006680">HLA Antigens</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010455">Peptides</NameOfSubstance></Chemical><Chemical><RegistryNumber>82115-62-6</RegistryNumber><NameOfSubstance UI="D007371">Interferon-gamma</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000328" MajorTopicYN="N">Adult</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001681" MajorTopicYN="Y">Biological Assay</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005260" MajorTopicYN="N">Female</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015683" MajorTopicYN="N">Gene Products, gag</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006678" MajorTopicYN="N">HIV</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006680" MajorTopicYN="N">HLA Antigens</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007371" MajorTopicYN="N">Interferon-gamma</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008297" MajorTopicYN="N">Male</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008875" MajorTopicYN="N">Middle Aged</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010455" MajorTopicYN="N">Peptides</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013602" MajorTopicYN="N">T-Lymphocytes, Cytotoxic</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2003</Year><Month>2</Month><Day>28</Day><Hour>4</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2003</Year><Month>8</Month><Day>2</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2003</Year><Month>2</Month><Day>28</Day><Hour>4</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">12606814</ArticleId><ArticleId IdType="pii">S1528365802207983</ArticleId><ArticleId IdType="pmc">PMC2039964</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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