Computer-based design of an HLA-haplotype and HIV-clade independent cytotoxic T-lymphocyte assay for monitoring HIV-specific immunity.
Identifieur interne : 002469 ( PubMed/Corpus ); précédent : 002468; suivant : 002470Computer-based design of an HLA-haplotype and HIV-clade independent cytotoxic T-lymphocyte assay for monitoring HIV-specific immunity.
Auteurs : Massimo Amicosante ; Cristiana Gioia ; Carla Montesano ; Rita Casetti ; Simone Topino ; Gianpiero D'Offizi ; Giulia Cappelli ; Giuseppe Ippolito ; Vittorio Colizzi ; Fabrizio Poccia ; Leopoldo P. PucilloSource :
- Molecular medicine (Cambridge, Mass.) [ 1076-1551 ] ; 2002.
English descriptors
- KwdEn :
- Adult, Amino Acid Sequence, Biological Assay, Female, Gene Products, gag (immunology), HIV (immunology), HLA Antigens (immunology), Humans, Interferon-gamma (metabolism), Male, Middle Aged, Molecular Sequence Data, Peptides (immunology), T-Lymphocytes, Cytotoxic (immunology), T-Lymphocytes, Cytotoxic (metabolism).
- MESH :
- chemical , immunology : Gene Products, gag, HLA Antigens, Peptides.
- immunology : HIV, T-Lymphocytes, Cytotoxic.
- chemical , metabolism : Interferon-gamma, T-Lymphocytes, Cytotoxic.
- Adult, Amino Acid Sequence, Biological Assay, Female, Humans, Male, Middle Aged, Molecular Sequence Data.
Abstract
Human immunodeficiency virus (HIV)- specific CD8-positive cytotoxic T-lymphocytes (CTL) play a key role in controlling HIV infection. Monitoring CTL response could be clinically relevant during structured therapy interruption (STI), HIV exposure, and vaccine trials. However, HLA patients' restriction and HIV variability limited the development of a CTL assay with broad specificity.
PubMed: 12606814
Links to Exploration step
pubmed:12606814Le document en format XML
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<author><name sortKey="Amicosante, Massimo" sort="Amicosante, Massimo" uniqKey="Amicosante M" first="Massimo" last="Amicosante">Massimo Amicosante</name>
<affiliation><nlm:affiliation>Laboratory of Clinical Pathology, National InstiTute for Infectious Disease, Lazzaro Spallanzani, Rome, Italy. amicosante@inmi.it</nlm:affiliation>
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<author><name sortKey="Gioia, Cristiana" sort="Gioia, Cristiana" uniqKey="Gioia C" first="Cristiana" last="Gioia">Cristiana Gioia</name>
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<author><name sortKey="Montesano, Carla" sort="Montesano, Carla" uniqKey="Montesano C" first="Carla" last="Montesano">Carla Montesano</name>
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<author><name sortKey="Casetti, Rita" sort="Casetti, Rita" uniqKey="Casetti R" first="Rita" last="Casetti">Rita Casetti</name>
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<term>HIV (immunology)</term>
<term>HLA Antigens (immunology)</term>
<term>Humans</term>
<term>Interferon-gamma (metabolism)</term>
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<term>Middle Aged</term>
<term>Molecular Sequence Data</term>
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<term>T-Lymphocytes, Cytotoxic (immunology)</term>
<term>T-Lymphocytes, Cytotoxic (metabolism)</term>
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<term>T-Lymphocytes, Cytotoxic</term>
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<term>Biological Assay</term>
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<front><div type="abstract" xml:lang="en">Human immunodeficiency virus (HIV)- specific CD8-positive cytotoxic T-lymphocytes (CTL) play a key role in controlling HIV infection. Monitoring CTL response could be clinically relevant during structured therapy interruption (STI), HIV exposure, and vaccine trials. However, HLA patients' restriction and HIV variability limited the development of a CTL assay with broad specificity.</div>
</front>
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<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">12606814</PMID>
<DateCompleted><Year>2003</Year>
<Month>08</Month>
<Day>01</Day>
</DateCompleted>
<DateRevised><Year>2018</Year>
<Month>08</Month>
<Day>21</Day>
</DateRevised>
<Article PubModel="Print"><Journal><ISSN IssnType="Print">1076-1551</ISSN>
<JournalIssue CitedMedium="Print"><Volume>8</Volume>
<Issue>12</Issue>
<PubDate><Year>2002</Year>
<Month>Dec</Month>
</PubDate>
</JournalIssue>
<Title>Molecular medicine (Cambridge, Mass.)</Title>
<ISOAbbreviation>Mol. Med.</ISOAbbreviation>
</Journal>
<ArticleTitle>Computer-based design of an HLA-haplotype and HIV-clade independent cytotoxic T-lymphocyte assay for monitoring HIV-specific immunity.</ArticleTitle>
<Pagination><MedlinePgn>798-807</MedlinePgn>
</Pagination>
<Abstract><AbstractText Label="BACKGROUND" NlmCategory="BACKGROUND">Human immunodeficiency virus (HIV)- specific CD8-positive cytotoxic T-lymphocytes (CTL) play a key role in controlling HIV infection. Monitoring CTL response could be clinically relevant during structured therapy interruption (STI), HIV exposure, and vaccine trials. However, HLA patients' restriction and HIV variability limited the development of a CTL assay with broad specificity.</AbstractText>
<AbstractText Label="MATERIALS AND METHODS" NlmCategory="METHODS">We designed an HLA-class I/HIV-1 clade independent assay for assessing HIV- specific CTL by using a computer-assisted selection ofthe CTL epitopes. Twenty-eight 15-mers were selected by peptide-binding motifs analysis using different databases (HIV-Immunology Database, SYFPEITHI, BIMAS). Altogether they putatively bind to more than 90% of HLA haplotypes in different populations, with an overall HIV-1 variability below 9%. The peptide pool was used as an antigen in an intracellular cytokine staining (ICS) assay for quantifying HIV-specific CTL response.</AbstractText>
<AbstractText Label="RESULTS" NlmCategory="RESULTS">The test can be performed using both fresh and cryopreserved peripheral blood mononuclear cells (PBMC), whereas GAG protein as antigen works only on fresh PBMC. A significantly higher CTL response with respect to HIV-negative controls was detected in all HIV-1 infected subjects of two groups of patients with different ethnicities (Caucasians and Africans) and coming from areas with different HIV-1 clade prevalences (clade B and A/G, respectively). In Caucasian patients, after month of STI, the number of HIV-1 specific CTL (2,896 +/- 2,780 IFN-gamma specific CD8 cells/ml) was significantly higher than that found at enrolment (2,125 +/- 4,426 IFN-gamma specific CD8 cells/ml, p< 0.05).</AbstractText>
<AbstractText Label="CONCLUSIONS" NlmCategory="CONCLUSIONS">These data indicate that this CTL assay is broadly specific and could represent a useful clinical tool for HIV immunodiagnostic independent of HLA-haplotype and HIV-clade variabilities.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Amicosante</LastName>
<ForeName>Massimo</ForeName>
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<AffiliationInfo><Affiliation>Laboratory of Clinical Pathology, National InstiTute for Infectious Disease, Lazzaro Spallanzani, Rome, Italy. amicosante@inmi.it</Affiliation>
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</Author>
<Author ValidYN="Y"><LastName>Gioia</LastName>
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<Author ValidYN="Y"><LastName>Montesano</LastName>
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<Author ValidYN="Y"><LastName>Casetti</LastName>
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<Author ValidYN="Y"><LastName>Topino</LastName>
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<QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName>
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<MeshHeading><DescriptorName UI="D013602" MajorTopicYN="N">T-Lymphocytes, Cytotoxic</DescriptorName>
<QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName>
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