Structural characterization of melphalan modified 2'-oligodeoxynucleotides by miniaturized LC-ES MS/MS.
Identifieur interne : 002399 ( PubMed/Curation ); précédent : 002398; suivant : 002400Structural characterization of melphalan modified 2'-oligodeoxynucleotides by miniaturized LC-ES MS/MS.
Auteurs : Bart Van Den Driessche [Belgique] ; Filip Lemière ; Walter Van Dongen ; Eddy L. EsmansSource :
- Journal of the American Society for Mass Spectrometry [ 1044-0305 ] ; 2004.
Descripteurs français
- KwdFr :
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : Antineoplastic Agents, Alkylating, Melphalan, Oligodeoxyribonucleotides.
- chemical , genetics : Oligodeoxyribonucleotides.
- methods : Spectrometry, Mass, Electrospray Ionization.
- Alkylation, Base Sequence, Chromatography, Molecular Structure, Molecular Weight.
Abstract
In this study a miniaturized LC coupled to electrospray tandem mass spectrometry was used to analyze modifications originating from the interaction between the chemotherapeutic agent melphalan and 2'-oligodeoxynucleotides. Low energy CAD product ion spectra gave information about the specificity of melphalan alkylation with regard to certain DNA sequences. These data can be very useful to estimate the risk in the development of secondary leukaemia as a result of a melphalan cure. In the study of the interaction between melphalan and d(GG), differentiation could be made between alkylation on the 5'-side and alkylation on the 3'-side, because of the presence or absence of the alkylated w1 fragment in the low energy CAD spectra. In the other di-mers alkylation specificity for the different bases could be observed. Melphalan alkylation occurs in the sequence G > A > C > T. The study of the alkylated d(GGGG) revealed the presence of mainly 5'-end alkylation. Furthermore studies were performed which investigated other melphalan treated di-, tetra-, hepta-, and octa-mers.
DOI: 10.1016/j.jasms.2003.12.005
PubMed: 15047061
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Esmans</name></author></analytic><series><title level="j">Journal of the American Society for Mass Spectrometry</title><idno type="ISSN">1044-0305</idno><imprint><date when="2004" type="published">2004</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Alkylation</term><term>Antineoplastic Agents, Alkylating (chemistry)</term><term>Base Sequence</term><term>Chromatography</term><term>Melphalan (chemistry)</term><term>Molecular Structure</term><term>Molecular Weight</term><term>Oligodeoxyribonucleotides (chemistry)</term><term>Oligodeoxyribonucleotides (genetics)</term><term>Spectrometry, Mass, Electrospray Ionization (methods)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Alkylation</term><term>Antinéoplasiques alcoylants ()</term><term>Chromatographie</term><term>Masse moléculaire</term><term>Melphalan ()</term><term>Oligodésoxyribonucléotides ()</term><term>Oligodésoxyribonucléotides (génétique)</term><term>Spectrométrie de masse ESI ()</term><term>Structure moléculaire</term><term>Séquence nucléotidique</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Antineoplastic Agents, Alkylating</term><term>Melphalan</term><term>Oligodeoxyribonucleotides</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Oligodeoxyribonucleotides</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Oligodésoxyribonucléotides</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Spectrometry, Mass, Electrospray Ionization</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Alkylation</term><term>Base Sequence</term><term>Chromatography</term><term>Molecular Structure</term><term>Molecular Weight</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Alkylation</term><term>Antinéoplasiques alcoylants</term><term>Chromatographie</term><term>Masse moléculaire</term><term>Melphalan</term><term>Oligodésoxyribonucléotides</term><term>Spectrométrie de masse ESI</term><term>Structure moléculaire</term><term>Séquence nucléotidique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In this study a miniaturized LC coupled to electrospray tandem mass spectrometry was used to analyze modifications originating from the interaction between the chemotherapeutic agent melphalan and 2'-oligodeoxynucleotides. Low energy CAD product ion spectra gave information about the specificity of melphalan alkylation with regard to certain DNA sequences. These data can be very useful to estimate the risk in the development of secondary leukaemia as a result of a melphalan cure. In the study of the interaction between melphalan and d(GG), differentiation could be made between alkylation on the 5'-side and alkylation on the 3'-side, because of the presence or absence of the alkylated w1 fragment in the low energy CAD spectra. In the other di-mers alkylation specificity for the different bases could be observed. Melphalan alkylation occurs in the sequence G > A > C > T. The study of the alkylated d(GGGG) revealed the presence of mainly 5'-end alkylation. Furthermore studies were performed which investigated other melphalan treated di-, tetra-, hepta-, and octa-mers.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15047061</PMID><DateCompleted><Year>2004</Year><Month>05</Month><Day>25</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">1044-0305</ISSN><JournalIssue CitedMedium="Print"><Volume>15</Volume><Issue>4</Issue><PubDate><Year>2004</Year><Month>Apr</Month></PubDate></JournalIssue><Title>Journal of the American Society for Mass Spectrometry</Title><ISOAbbreviation>J. Am. Soc. Mass Spectrom.</ISOAbbreviation></Journal><ArticleTitle>Structural characterization of melphalan modified 2'-oligodeoxynucleotides by miniaturized LC-ES MS/MS.</ArticleTitle><Pagination><MedlinePgn>568-79</MedlinePgn></Pagination><Abstract><AbstractText>In this study a miniaturized LC coupled to electrospray tandem mass spectrometry was used to analyze modifications originating from the interaction between the chemotherapeutic agent melphalan and 2'-oligodeoxynucleotides. Low energy CAD product ion spectra gave information about the specificity of melphalan alkylation with regard to certain DNA sequences. These data can be very useful to estimate the risk in the development of secondary leukaemia as a result of a melphalan cure. In the study of the interaction between melphalan and d(GG), differentiation could be made between alkylation on the 5'-side and alkylation on the 3'-side, because of the presence or absence of the alkylated w1 fragment in the low energy CAD spectra. In the other di-mers alkylation specificity for the different bases could be observed. Melphalan alkylation occurs in the sequence G > A > C > T. The study of the alkylated d(GGGG) revealed the presence of mainly 5'-end alkylation. Furthermore studies were performed which investigated other melphalan treated di-, tetra-, hepta-, and octa-mers.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Van den Driessche</LastName><ForeName>Bart</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>Nucleoside Research and Mass Spectrometry Unit, Department of Chemistry, University of Antwerp, Antwerp, Belgium.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lemière</LastName><ForeName>Filip</ForeName><Initials>F</Initials></Author><Author ValidYN="Y"><LastName>van Dongen</LastName><ForeName>Walter</ForeName><Initials>W</Initials></Author><Author ValidYN="Y"><LastName>Esmans</LastName><ForeName>Eddy L</ForeName><Initials>EL</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. 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