Effects of side chain configuration and backbone spacing on the gene delivery properties of lysine-derived cationic polymers.
Identifieur interne : 002334 ( PubMed/Curation ); précédent : 002333; suivant : 002335Effects of side chain configuration and backbone spacing on the gene delivery properties of lysine-derived cationic polymers.
Auteurs : Sarah E. Eldred [États-Unis] ; Margaret R. Pancost ; Karin M. Otte ; David Rozema ; Shannon S. Stahl ; Samuel H. GellmanSource :
- Bioconjugate chemistry [ 1043-1802 ]
Descripteurs français
- KwdFr :
- MESH :
- administration et posologie : ADN.
- génétique : ADN.
- pharmacologie : Chloroquine, Transfection.
- Animaux, Cations, Humains, Lignée cellulaire, Lysine, Polymères, Structure moléculaire, Transfection.
English descriptors
- KwdEn :
- MESH :
- chemical , administration & dosage : DNA.
- chemical , chemistry : Cations, Lysine, Polymers.
- chemical , genetics : DNA.
- chemical , pharmacology : Chloroquine.
- instrumentation : Transfection.
- methods : Transfection.
- Animals, Cell Line, Chlorocebus aethiops, Humans, Molecular Structure.
Abstract
A series of lysine-based oligomers (18 residues) that differ in side chain configuration or side chain spacing along the backbone was tested for DNA transfection activity. Although materials constructed from lysine are not the most effective polymeric transfection agents, we have chosen L-lysine-based molecules as a starting point because this system allows us to examine the functional effects of incremental changes in polycation structure. The oligomer constructed from beta(3)-homolysine (beta(3)-hLys) and that from alpha-D-lysine were superior to an alpha-L-lysine 18-mer in gene delivery assays. This improved activity is attributed to the fact that the alpha-L-peptide is a protease substrate while the other 18-mers are not. This conclusion is supported by the effects of chloroquine on transfection activity, based on the protease inhibition activity of chloroquine. To our knowledge, these results represent the first direct comparison of a D-lysine oligomer with an L-lysine oligomer in the context of gene delivery. Poly(beta(3)-hLys) was synthesized from the ring opening polymerization of the corresponding lactam. The DNA transfection ability of this polymer was compared with that of commercially available poly(L-lysine) (PLL). In each case the polymer was more active than the corresponding oligomer.
DOI: 10.1021/bc050017c
PubMed: 15898739
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: Pour aller vers cette notice dans l'étape Curation :002334
Links to Exploration step
pubmed:15898739Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Effects of side chain configuration and backbone spacing on the gene delivery properties of lysine-derived cationic polymers.</title><author><name sortKey="Eldred, Sarah E" sort="Eldred, Sarah E" uniqKey="Eldred S" first="Sarah E" last="Eldred">Sarah E. Eldred</name><affiliation wicri:level="1"><nlm:affiliation>Department of Chemistry, University of Wisconsin-Madison, Wisconsin 53706, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Chemistry, University of Wisconsin-Madison, Wisconsin 53706</wicri:regionArea></affiliation></author><author><name sortKey="Pancost, Margaret R" sort="Pancost, Margaret R" uniqKey="Pancost M" first="Margaret R" last="Pancost">Margaret R. Pancost</name></author><author><name sortKey="Otte, Karin M" sort="Otte, Karin M" uniqKey="Otte K" first="Karin M" last="Otte">Karin M. Otte</name></author><author><name sortKey="Rozema, David" sort="Rozema, David" uniqKey="Rozema D" first="David" last="Rozema">David Rozema</name></author><author><name sortKey="Stahl, Shannon S" sort="Stahl, Shannon S" uniqKey="Stahl S" first="Shannon S" last="Stahl">Shannon S. Stahl</name></author><author><name sortKey="Gellman, Samuel H" sort="Gellman, Samuel H" uniqKey="Gellman S" first="Samuel H" last="Gellman">Samuel H. Gellman</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="????"><PubDate><MedlineDate>2005 May-Jun</MedlineDate></PubDate></date><idno type="RBID">pubmed:15898739</idno><idno type="pmid">15898739</idno><idno type="doi">10.1021/bc050017c</idno><idno type="wicri:Area/PubMed/Corpus">002334</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002334</idno><idno type="wicri:Area/PubMed/Curation">002334</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002334</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Effects of side chain configuration and backbone spacing on the gene delivery properties of lysine-derived cationic polymers.</title><author><name sortKey="Eldred, Sarah E" sort="Eldred, Sarah E" uniqKey="Eldred S" first="Sarah E" last="Eldred">Sarah E. Eldred</name><affiliation wicri:level="1"><nlm:affiliation>Department of Chemistry, University of Wisconsin-Madison, Wisconsin 53706, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Chemistry, University of Wisconsin-Madison, Wisconsin 53706</wicri:regionArea></affiliation></author><author><name sortKey="Pancost, Margaret R" sort="Pancost, Margaret R" uniqKey="Pancost M" first="Margaret R" last="Pancost">Margaret R. Pancost</name></author><author><name sortKey="Otte, Karin M" sort="Otte, Karin M" uniqKey="Otte K" first="Karin M" last="Otte">Karin M. Otte</name></author><author><name sortKey="Rozema, David" sort="Rozema, David" uniqKey="Rozema D" first="David" last="Rozema">David Rozema</name></author><author><name sortKey="Stahl, Shannon S" sort="Stahl, Shannon S" uniqKey="Stahl S" first="Shannon S" last="Stahl">Shannon S. Stahl</name></author><author><name sortKey="Gellman, Samuel H" sort="Gellman, Samuel H" uniqKey="Gellman S" first="Samuel H" last="Gellman">Samuel H. Gellman</name></author></analytic><series><title level="j">Bioconjugate chemistry</title><idno type="ISSN">1043-1802</idno></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Cations (chemistry)</term><term>Cell Line</term><term>Chlorocebus aethiops</term><term>Chloroquine (pharmacology)</term><term>DNA (administration & dosage)</term><term>DNA (genetics)</term><term>Humans</term><term>Lysine (chemistry)</term><term>Molecular Structure</term><term>Polymers (chemistry)</term><term>Transfection (instrumentation)</term><term>Transfection (methods)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN (administration et posologie)</term><term>ADN (génétique)</term><term>Animaux</term><term>Cations ()</term><term>Chloroquine (pharmacologie)</term><term>Humains</term><term>Lignée cellulaire</term><term>Lysine ()</term><term>Polymères ()</term><term>Structure moléculaire</term><term>Transfection ()</term><term>Transfection (instrumentation)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="administration & dosage" xml:lang="en"><term>DNA</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Cations</term><term>Lysine</term><term>Polymers</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Chloroquine</term></keywords><keywords scheme="MESH" qualifier="administration et posologie" xml:lang="fr"><term>ADN</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN</term></keywords><keywords scheme="MESH" qualifier="instrumentation" xml:lang="en"><term>Transfection</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Transfection</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Chloroquine</term><term>Transfection</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cell Line</term><term>Chlorocebus aethiops</term><term>Humans</term><term>Molecular Structure</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Cations</term><term>Humains</term><term>Lignée cellulaire</term><term>Lysine</term><term>Polymères</term><term>Structure moléculaire</term><term>Transfection</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A series of lysine-based oligomers (18 residues) that differ in side chain configuration or side chain spacing along the backbone was tested for DNA transfection activity. Although materials constructed from lysine are not the most effective polymeric transfection agents, we have chosen L-lysine-based molecules as a starting point because this system allows us to examine the functional effects of incremental changes in polycation structure. The oligomer constructed from beta(3)-homolysine (beta(3)-hLys) and that from alpha-D-lysine were superior to an alpha-L-lysine 18-mer in gene delivery assays. This improved activity is attributed to the fact that the alpha-L-peptide is a protease substrate while the other 18-mers are not. This conclusion is supported by the effects of chloroquine on transfection activity, based on the protease inhibition activity of chloroquine. To our knowledge, these results represent the first direct comparison of a D-lysine oligomer with an L-lysine oligomer in the context of gene delivery. Poly(beta(3)-hLys) was synthesized from the ring opening polymerization of the corresponding lactam. The DNA transfection ability of this polymer was compared with that of commercially available poly(L-lysine) (PLL). In each case the polymer was more active than the corresponding oligomer.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15898739</PMID><DateCompleted><Year>2005</Year><Month>09</Month><Day>08</Day></DateCompleted><DateRevised><Year>2019</Year><Month>12</Month><Day>10</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">1043-1802</ISSN><JournalIssue CitedMedium="Print"><Volume>16</Volume><Issue>3</Issue><PubDate><MedlineDate>2005 May-Jun</MedlineDate></PubDate></JournalIssue><Title>Bioconjugate chemistry</Title><ISOAbbreviation>Bioconjug. Chem.</ISOAbbreviation></Journal><ArticleTitle>Effects of side chain configuration and backbone spacing on the gene delivery properties of lysine-derived cationic polymers.</ArticleTitle><Pagination><MedlinePgn>694-9</MedlinePgn></Pagination><Abstract><AbstractText>A series of lysine-based oligomers (18 residues) that differ in side chain configuration or side chain spacing along the backbone was tested for DNA transfection activity. Although materials constructed from lysine are not the most effective polymeric transfection agents, we have chosen L-lysine-based molecules as a starting point because this system allows us to examine the functional effects of incremental changes in polycation structure. The oligomer constructed from beta(3)-homolysine (beta(3)-hLys) and that from alpha-D-lysine were superior to an alpha-L-lysine 18-mer in gene delivery assays. This improved activity is attributed to the fact that the alpha-L-peptide is a protease substrate while the other 18-mers are not. This conclusion is supported by the effects of chloroquine on transfection activity, based on the protease inhibition activity of chloroquine. To our knowledge, these results represent the first direct comparison of a D-lysine oligomer with an L-lysine oligomer in the context of gene delivery. Poly(beta(3)-hLys) was synthesized from the ring opening polymerization of the corresponding lactam. The DNA transfection ability of this polymer was compared with that of commercially available poly(L-lysine) (PLL). In each case the polymer was more active than the corresponding oligomer.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Eldred</LastName><ForeName>Sarah E</ForeName><Initials>SE</Initials><AffiliationInfo><Affiliation>Department of Chemistry, University of Wisconsin-Madison, Wisconsin 53706, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Pancost</LastName><ForeName>Margaret R</ForeName><Initials>MR</Initials></Author><Author ValidYN="Y"><LastName>Otte</LastName><ForeName>Karin M</ForeName><Initials>KM</Initials></Author><Author ValidYN="Y"><LastName>Rozema</LastName><ForeName>David</ForeName><Initials>D</Initials></Author><Author ValidYN="Y"><LastName>Stahl</LastName><ForeName>Shannon S</ForeName><Initials>SS</Initials></Author><Author ValidYN="Y"><LastName>Gellman</LastName><ForeName>Samuel H</ForeName><Initials>SH</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Bioconjug Chem</MedlineTA><NlmUniqueID>9010319</NlmUniqueID><ISSNLinking>1043-1802</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D002412">Cations</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011108">Polymers</NameOfSubstance></Chemical><Chemical><RegistryNumber>886U3H6UFF</RegistryNumber><NameOfSubstance UI="D002738">Chloroquine</NameOfSubstance></Chemical><Chemical><RegistryNumber>9007-49-2</RegistryNumber><NameOfSubstance UI="D004247">DNA</NameOfSubstance></Chemical><Chemical><RegistryNumber>K3Z4F929H6</RegistryNumber><NameOfSubstance UI="D008239">Lysine</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002412" MajorTopicYN="N">Cations</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002460" MajorTopicYN="N">Cell Line</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002522" MajorTopicYN="N">Chlorocebus aethiops</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002738" MajorTopicYN="N">Chloroquine</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004247" MajorTopicYN="N">DNA</DescriptorName><QualifierName UI="Q000008" MajorTopicYN="Y">administration & dosage</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008239" MajorTopicYN="N">Lysine</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015394" MajorTopicYN="N">Molecular Structure</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011108" MajorTopicYN="N">Polymers</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014162" MajorTopicYN="N">Transfection</DescriptorName><QualifierName UI="Q000295" MajorTopicYN="Y">instrumentation</QualifierName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2005</Year><Month>5</Month><Day>19</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2005</Year><Month>9</Month><Day>9</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2005</Year><Month>5</Month><Day>19</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">15898739</ArticleId><ArticleId IdType="doi">10.1021/bc050017c</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/PubMed/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002334 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/PubMed/Curation/biblio.hfd -nk 002334 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= MersV1
|flux= PubMed
|étape= Curation
|type= RBID
|clé= pubmed:15898739
|texte= Effects of side chain configuration and backbone spacing on the gene delivery properties of lysine-derived cationic polymers.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Curation/RBID.i -Sk "pubmed:15898739" \
| HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Curation/biblio.hfd \
| NlmPubMed2Wicri -a MersV1
| This area was generated with Dilib version V0.6.33. |  |