Separation of modified 2'-deoxyoligonucleotides using ion-pairing reversed-phase HPLC.
Identifieur interne : 002333 ( PubMed/Curation ); précédent : 002332; suivant : 002334Separation of modified 2'-deoxyoligonucleotides using ion-pairing reversed-phase HPLC.
Auteurs : Stacy L. Gelhaus [États-Unis] ; William R. LacourseSource :
- Journal of chromatography. B, Analytical technologies in the biomedical and life sciences [ 1570-0232 ] ; 2005.
Descripteurs français
- KwdFr :
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical , isolation & purification : Oligonucleotides.
- chemical : DNA Primers.
- methods : Chromatography, High Pressure Liquid.
- Base Sequence, Chromatography, Ion Exchange, Electrophoresis, Capillary.
Abstract
A group of 18-mers of the same base sequence, but with differing alkyl modifications is used to investigate effects of these modifications on retention of oligonucleotides using ion-pairing reversed-phase liquid chromatography (IP-RPLC). It is shown that IP-RPLC is able to distinguish between oligonucleotides differing only by a single alkyl group. The identity of the nucleobase and position and length of the alkyl adduct affect retention of the oligonucleotide. These separation phenomena result from changes in charge and hydrophobicity upon alkylation. As demonstrated in this paper; chromatographic selectivity, unique to IP-RPLC, greatly facilitates the purification process of modified oligonucleotides.
DOI: 10.1016/j.jchromb.2005.02.024
PubMed: 15899369
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pubmed:15899369Le document en format XML
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<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Chemistry and Biochemistry, University of Maryland, 1000 Hilltop Circle, Baltimore, MD 21250</wicri:regionArea>
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<front><div type="abstract" xml:lang="en">A group of 18-mers of the same base sequence, but with differing alkyl modifications is used to investigate effects of these modifications on retention of oligonucleotides using ion-pairing reversed-phase liquid chromatography (IP-RPLC). It is shown that IP-RPLC is able to distinguish between oligonucleotides differing only by a single alkyl group. The identity of the nucleobase and position and length of the alkyl adduct affect retention of the oligonucleotide. These separation phenomena result from changes in charge and hydrophobicity upon alkylation. As demonstrated in this paper; chromatographic selectivity, unique to IP-RPLC, greatly facilitates the purification process of modified oligonucleotides.</div>
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<Abstract><AbstractText>A group of 18-mers of the same base sequence, but with differing alkyl modifications is used to investigate effects of these modifications on retention of oligonucleotides using ion-pairing reversed-phase liquid chromatography (IP-RPLC). It is shown that IP-RPLC is able to distinguish between oligonucleotides differing only by a single alkyl group. The identity of the nucleobase and position and length of the alkyl adduct affect retention of the oligonucleotide. These separation phenomena result from changes in charge and hydrophobicity upon alkylation. As demonstrated in this paper; chromatographic selectivity, unique to IP-RPLC, greatly facilitates the purification process of modified oligonucleotides.</AbstractText>
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