Bacterial Outer Membrane Vesicles (OMVs)-based Dual Vaccine for Influenza A H1N1 Virus and MERS-CoV.
Identifieur interne : 000519 ( PubMed/Curation ); précédent : 000518; suivant : 000520Bacterial Outer Membrane Vesicles (OMVs)-based Dual Vaccine for Influenza A H1N1 Virus and MERS-CoV.
Auteurs : Mahmoud M. Shehata [Égypte] ; Ahmed Mostafa [Égypte] ; Lisa Teubner [Allemagne] ; Sara H. Mahmoud [Égypte] ; Ahmed Kandeil [Égypte] ; Rabeh Elshesheny [Égypte] ; Renate Frantz [Allemagne] ; Luigi La Pietra [Allemagne] ; Stephan Pleschka [Allemagne] ; Ahmed Osman [Égypte] ; Ghazi Kayali [États-Unis] ; Trinad Chakraborty [Allemagne] ; Mohamed A. Ali [Égypte] ; Mobarak Abu Mraheil [Allemagne]Source :
- Vaccines [ 2076-393X ] ; 2019.
Abstract
Vaccination is the most functional medical intervention to prophylactically control severe diseases caused by human-to-human or animal-to-human transmissible viral pathogens. Annually, seasonal influenza epidemics attack human populations leading to 290-650 thousand deaths/year worldwide. Recently, a novel Middle East Respiratory Syndrome Coronavirus emerged. Together, those two viruses present a significant public health burden in areas where they circulate. Herein, we generated a bacterial outer membrane vesicles (OMVs)-based vaccine presenting the antigenic stable chimeric fusion protein of the H1-type haemagglutinin (HA) of the pandemic influenza A virus (H1N1) strain from 2009 (H1N1pdm09) and the receptor binding domain (RBD) of the Middle East Respiratory Syndrome Coronavirus (MERS-CoV) (OMVs-H1/RBD). Our results showed that the chimeric antigen could induce specific neutralizing antibodies against both strains leading to protection of immunized mice against H1N1pdm09 and efficient neutralization of MERS-CoV. This study demonstrate that OMVs-based vaccines presenting viral antigens provide a safe and reliable approach to protect against two different viral infections.
DOI: 10.3390/vaccines7020046
PubMed: 31141982
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Mahmoud.Shehata@human-link.org.</nlm:affiliation><country xml:lang="fr">Égypte</country><wicri:regionArea>Center of Scientific Excellence for Influenza Viruses, Environmental Research Division, National Research Centre (NRC), Cairo 12622</wicri:regionArea></affiliation></author><author><name sortKey="Mostafa, Ahmed" sort="Mostafa, Ahmed" uniqKey="Mostafa A" first="Ahmed" last="Mostafa">Ahmed Mostafa</name><affiliation wicri:level="1"><nlm:affiliation>Center of Scientific Excellence for Influenza Viruses, Environmental Research Division, National Research Centre (NRC), Cairo 12622, Egypt. ahmed_elsayed@daad-alumni.de.</nlm:affiliation><country xml:lang="fr">Égypte</country><wicri:regionArea>Center of Scientific Excellence for Influenza Viruses, Environmental Research Division, National Research Centre (NRC), Cairo 12622</wicri:regionArea></affiliation></author><author><name sortKey="Teubner, Lisa" sort="Teubner, Lisa" uniqKey="Teubner L" first="Lisa" last="Teubner">Lisa Teubner</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Medical Microbiology, German Center for Infection Research (DZIF), Partner Site Giessen-Marburg-Langen Site, Justus-Liebig University Giessen, 35392 Giessen , Germany. lisa.teubner@mikrobio.med.uni-giessen.de.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Medical Microbiology, German Center for Infection Research (DZIF), Partner Site Giessen-Marburg-Langen Site, Justus-Liebig University Giessen, 35392 Giessen </wicri:regionArea></affiliation></author><author><name sortKey="Mahmoud, Sara H" sort="Mahmoud, Sara H" uniqKey="Mahmoud S" first="Sara H" last="Mahmoud">Sara H. 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Stephan.Pleschka@viro.med.uni-giessen.de.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Medical Virology, Justus-Liebig University Giessen, 35392 Giessen </wicri:regionArea></affiliation></author><author><name sortKey="Osman, Ahmed" sort="Osman, Ahmed" uniqKey="Osman A" first="Ahmed" last="Osman">Ahmed Osman</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biochemistry, Faculty of Science, Ain Shams University, Cairo 38105, Egypt. aoegiza@yahoo.com.</nlm:affiliation><country xml:lang="fr">Égypte</country><wicri:regionArea>Department of Biochemistry, Faculty of Science, Ain Shams University, Cairo 38105</wicri:regionArea></affiliation></author><author><name sortKey="Kayali, Ghazi" sort="Kayali, Ghazi" uniqKey="Kayali G" first="Ghazi" last="Kayali">Ghazi Kayali</name><affiliation wicri:level="1"><nlm:affiliation>Department of Epidemiology, Human Genetics, and Environmental Sciences, University of Texas, Houston, TX 77030, USA. ghazi@human-link.org.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Epidemiology, Human Genetics, and Environmental Sciences, University of Texas, Houston, TX 77030</wicri:regionArea></affiliation></author><author><name sortKey="Chakraborty, Trinad" sort="Chakraborty, Trinad" uniqKey="Chakraborty T" first="Trinad" last="Chakraborty">Trinad Chakraborty</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Medical Microbiology, German Center for Infection Research (DZIF), Partner Site Giessen-Marburg-Langen Site, Justus-Liebig University Giessen, 35392 Giessen , Germany. trinad.chakraborty@mikrobio.med.uni-giessen.de.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Medical Microbiology, German Center for Infection Research (DZIF), Partner Site Giessen-Marburg-Langen Site, Justus-Liebig University Giessen, 35392 Giessen </wicri:regionArea></affiliation></author><author><name sortKey="Ali, Mohamed A" sort="Ali, Mohamed A" uniqKey="Ali M" first="Mohamed A" last="Ali">Mohamed A. Ali</name><affiliation wicri:level="1"><nlm:affiliation>Center of Scientific Excellence for Influenza Viruses, Environmental Research Division, National Research Centre (NRC), Cairo 12622, Egypt. Mobarak.Mraheil@mikrobio.med.uni-giessen.de.</nlm:affiliation><country xml:lang="fr">Égypte</country><wicri:regionArea>Center of Scientific Excellence for Influenza Viruses, Environmental Research Division, National Research Centre (NRC), Cairo 12622</wicri:regionArea></affiliation></author><author><name sortKey="Mraheil, Mobarak Abu" sort="Mraheil, Mobarak Abu" uniqKey="Mraheil M" first="Mobarak Abu" last="Mraheil">Mobarak Abu Mraheil</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Medical Microbiology, German Center for Infection Research (DZIF), Partner Site Giessen-Marburg-Langen Site, Justus-Liebig University Giessen, 35392 Giessen , Germany. Mohamedahmedali2004@yahoo.com.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Medical Microbiology, German Center for Infection Research (DZIF), Partner Site Giessen-Marburg-Langen Site, Justus-Liebig University Giessen, 35392 Giessen </wicri:regionArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2019">2019</date><idno type="RBID">pubmed:31141982</idno><idno type="pmid">31141982</idno><idno type="doi">10.3390/vaccines7020046</idno><idno type="wicri:Area/PubMed/Corpus">000519</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000519</idno><idno type="wicri:Area/PubMed/Curation">000519</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000519</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Bacterial Outer Membrane Vesicles (OMVs)-based Dual Vaccine for Influenza A H1N1 Virus and MERS-CoV.</title><author><name sortKey="Shehata, Mahmoud M" sort="Shehata, Mahmoud M" uniqKey="Shehata M" first="Mahmoud M" last="Shehata">Mahmoud M. Shehata</name><affiliation wicri:level="1"><nlm:affiliation>Center of Scientific Excellence for Influenza Viruses, Environmental Research Division, National Research Centre (NRC), Cairo 12622, Egypt. Mahmoud.Shehata@human-link.org.</nlm:affiliation><country xml:lang="fr">Égypte</country><wicri:regionArea>Center of Scientific Excellence for Influenza Viruses, Environmental Research Division, National Research Centre (NRC), Cairo 12622</wicri:regionArea></affiliation></author><author><name sortKey="Mostafa, Ahmed" sort="Mostafa, Ahmed" uniqKey="Mostafa A" first="Ahmed" last="Mostafa">Ahmed Mostafa</name><affiliation wicri:level="1"><nlm:affiliation>Center of Scientific Excellence for Influenza Viruses, Environmental Research Division, National Research Centre (NRC), Cairo 12622, Egypt. ahmed_elsayed@daad-alumni.de.</nlm:affiliation><country xml:lang="fr">Égypte</country><wicri:regionArea>Center of Scientific Excellence for Influenza Viruses, Environmental Research Division, National Research Centre (NRC), Cairo 12622</wicri:regionArea></affiliation></author><author><name sortKey="Teubner, Lisa" sort="Teubner, Lisa" uniqKey="Teubner L" first="Lisa" last="Teubner">Lisa Teubner</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Medical Microbiology, German Center for Infection Research (DZIF), Partner Site Giessen-Marburg-Langen Site, Justus-Liebig University Giessen, 35392 Giessen , Germany. lisa.teubner@mikrobio.med.uni-giessen.de.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Medical Microbiology, German Center for Infection Research (DZIF), Partner Site Giessen-Marburg-Langen Site, Justus-Liebig University Giessen, 35392 Giessen </wicri:regionArea></affiliation></author><author><name sortKey="Mahmoud, Sara H" sort="Mahmoud, Sara H" uniqKey="Mahmoud S" first="Sara H" last="Mahmoud">Sara H. Mahmoud</name><affiliation wicri:level="1"><nlm:affiliation>Center of Scientific Excellence for Influenza Viruses, Environmental Research Division, National Research Centre (NRC), Cairo 12622, Egypt. sara.Hussein@human-link.org.</nlm:affiliation><country xml:lang="fr">Égypte</country><wicri:regionArea>Center of Scientific Excellence for Influenza Viruses, Environmental Research Division, National Research Centre (NRC), Cairo 12622</wicri:regionArea></affiliation></author><author><name sortKey="Kandeil, Ahmed" sort="Kandeil, Ahmed" uniqKey="Kandeil A" first="Ahmed" last="Kandeil">Ahmed Kandeil</name><affiliation wicri:level="1"><nlm:affiliation>Center of Scientific Excellence for Influenza Viruses, Environmental Research Division, National Research Centre (NRC), Cairo 12622, Egypt. ahmed.Kandeil@human-link.org.</nlm:affiliation><country xml:lang="fr">Égypte</country><wicri:regionArea>Center of Scientific Excellence for Influenza Viruses, Environmental Research Division, National Research Centre (NRC), Cairo 12622</wicri:regionArea></affiliation></author><author><name sortKey="Elshesheny, Rabeh" sort="Elshesheny, Rabeh" uniqKey="Elshesheny R" first="Rabeh" last="Elshesheny">Rabeh Elshesheny</name><affiliation wicri:level="1"><nlm:affiliation>Center of Scientific Excellence for Influenza Viruses, Environmental Research Division, National Research Centre (NRC), Cairo 12622, Egypt. rabeh.elshesheny@stjude.org.</nlm:affiliation><country xml:lang="fr">Égypte</country><wicri:regionArea>Center of Scientific Excellence for Influenza Viruses, Environmental Research Division, National Research Centre (NRC), Cairo 12622</wicri:regionArea></affiliation></author><author><name sortKey="Frantz, Renate" sort="Frantz, Renate" uniqKey="Frantz R" first="Renate" last="Frantz">Renate Frantz</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Medical Microbiology, German Center for Infection Research (DZIF), Partner Site Giessen-Marburg-Langen Site, Justus-Liebig University Giessen, 35392 Giessen , Germany. renate.frantz@mikrobio.med.uni-giessen.de.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Medical Microbiology, German Center for Infection Research (DZIF), Partner Site Giessen-Marburg-Langen Site, Justus-Liebig University Giessen, 35392 Giessen </wicri:regionArea></affiliation></author><author><name sortKey="La Pietra, Luigi" sort="La Pietra, Luigi" uniqKey="La Pietra L" first="Luigi" last="La Pietra">Luigi La Pietra</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Medical Microbiology, German Center for Infection Research (DZIF), Partner Site Giessen-Marburg-Langen Site, Justus-Liebig University Giessen, 35392 Giessen , Germany. luigi.La-pietra@mikrobio.med.uni-giessen.de.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Medical Microbiology, German Center for Infection Research (DZIF), Partner Site Giessen-Marburg-Langen Site, Justus-Liebig University Giessen, 35392 Giessen </wicri:regionArea></affiliation></author><author><name sortKey="Pleschka, Stephan" sort="Pleschka, Stephan" uniqKey="Pleschka S" first="Stephan" last="Pleschka">Stephan Pleschka</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Medical Virology, Justus-Liebig University Giessen, 35392 Giessen , Germany. Stephan.Pleschka@viro.med.uni-giessen.de.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Medical Virology, Justus-Liebig University Giessen, 35392 Giessen </wicri:regionArea></affiliation></author><author><name sortKey="Osman, Ahmed" sort="Osman, Ahmed" uniqKey="Osman A" first="Ahmed" last="Osman">Ahmed Osman</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biochemistry, Faculty of Science, Ain Shams University, Cairo 38105, Egypt. aoegiza@yahoo.com.</nlm:affiliation><country xml:lang="fr">Égypte</country><wicri:regionArea>Department of Biochemistry, Faculty of Science, Ain Shams University, Cairo 38105</wicri:regionArea></affiliation></author><author><name sortKey="Kayali, Ghazi" sort="Kayali, Ghazi" uniqKey="Kayali G" first="Ghazi" last="Kayali">Ghazi Kayali</name><affiliation wicri:level="1"><nlm:affiliation>Department of Epidemiology, Human Genetics, and Environmental Sciences, University of Texas, Houston, TX 77030, USA. ghazi@human-link.org.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Epidemiology, Human Genetics, and Environmental Sciences, University of Texas, Houston, TX 77030</wicri:regionArea></affiliation></author><author><name sortKey="Chakraborty, Trinad" sort="Chakraborty, Trinad" uniqKey="Chakraborty T" first="Trinad" last="Chakraborty">Trinad Chakraborty</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Medical Microbiology, German Center for Infection Research (DZIF), Partner Site Giessen-Marburg-Langen Site, Justus-Liebig University Giessen, 35392 Giessen , Germany. trinad.chakraborty@mikrobio.med.uni-giessen.de.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Medical Microbiology, German Center for Infection Research (DZIF), Partner Site Giessen-Marburg-Langen Site, Justus-Liebig University Giessen, 35392 Giessen </wicri:regionArea></affiliation></author><author><name sortKey="Ali, Mohamed A" sort="Ali, Mohamed A" uniqKey="Ali M" first="Mohamed A" last="Ali">Mohamed A. Ali</name><affiliation wicri:level="1"><nlm:affiliation>Center of Scientific Excellence for Influenza Viruses, Environmental Research Division, National Research Centre (NRC), Cairo 12622, Egypt. Mobarak.Mraheil@mikrobio.med.uni-giessen.de.</nlm:affiliation><country xml:lang="fr">Égypte</country><wicri:regionArea>Center of Scientific Excellence for Influenza Viruses, Environmental Research Division, National Research Centre (NRC), Cairo 12622</wicri:regionArea></affiliation></author><author><name sortKey="Mraheil, Mobarak Abu" sort="Mraheil, Mobarak Abu" uniqKey="Mraheil M" first="Mobarak Abu" last="Mraheil">Mobarak Abu Mraheil</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Medical Microbiology, German Center for Infection Research (DZIF), Partner Site Giessen-Marburg-Langen Site, Justus-Liebig University Giessen, 35392 Giessen , Germany. Mohamedahmedali2004@yahoo.com.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Medical Microbiology, German Center for Infection Research (DZIF), Partner Site Giessen-Marburg-Langen Site, Justus-Liebig University Giessen, 35392 Giessen </wicri:regionArea></affiliation></author></analytic><series><title level="j">Vaccines</title><idno type="ISSN">2076-393X</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Vaccination is the most functional medical intervention to prophylactically control severe diseases caused by human-to-human or animal-to-human transmissible viral pathogens. Annually, seasonal influenza epidemics attack human populations leading to 290-650 thousand deaths/year worldwide. Recently, a novel Middle East Respiratory Syndrome Coronavirus emerged. Together, those two viruses present a significant public health burden in areas where they circulate. Herein, we generated a bacterial outer membrane vesicles (OMVs)-based vaccine presenting the antigenic stable chimeric fusion protein of the H1-type haemagglutinin (HA) of the pandemic influenza A virus (H1N1) strain from 2009 (H1N1pdm09) and the receptor binding domain (RBD) of the Middle East Respiratory Syndrome Coronavirus (MERS-CoV) (OMVs-H1/RBD). Our results showed that the chimeric antigen could induce specific neutralizing antibodies against both strains leading to protection of immunized mice against H1N1pdm09 and efficient neutralization of MERS-CoV. This study demonstrate that OMVs-based vaccines presenting viral antigens provide a safe and reliable approach to protect against two different viral infections.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">31141982</PMID><DateRevised><Year>2020</Year><Month>02</Month><Day>25</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Print">2076-393X</ISSN><JournalIssue CitedMedium="Print"><Volume>7</Volume><Issue>2</Issue><PubDate><Year>2019</Year><Month>05</Month><Day>28</Day></PubDate></JournalIssue><Title>Vaccines</Title><ISOAbbreviation>Vaccines (Basel)</ISOAbbreviation></Journal><ArticleTitle>Bacterial Outer Membrane Vesicles (OMVs)-based Dual Vaccine for Influenza A H1N1 Virus and MERS-CoV.</ArticleTitle><ELocationID EIdType="pii" ValidYN="Y">E46</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.3390/vaccines7020046</ELocationID><Abstract><AbstractText>Vaccination is the most functional medical intervention to prophylactically control severe diseases caused by human-to-human or animal-to-human transmissible viral pathogens. Annually, seasonal influenza epidemics attack human populations leading to 290-650 thousand deaths/year worldwide. Recently, a novel Middle East Respiratory Syndrome Coronavirus emerged. Together, those two viruses present a significant public health burden in areas where they circulate. Herein, we generated a bacterial outer membrane vesicles (OMVs)-based vaccine presenting the antigenic stable chimeric fusion protein of the H1-type haemagglutinin (HA) of the pandemic influenza A virus (H1N1) strain from 2009 (H1N1pdm09) and the receptor binding domain (RBD) of the Middle East Respiratory Syndrome Coronavirus (MERS-CoV) (OMVs-H1/RBD). Our results showed that the chimeric antigen could induce specific neutralizing antibodies against both strains leading to protection of immunized mice against H1N1pdm09 and efficient neutralization of MERS-CoV. This study demonstrate that OMVs-based vaccines presenting viral antigens provide a safe and reliable approach to protect against two different viral infections.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Shehata</LastName><ForeName>Mahmoud M</ForeName><Initials>MM</Initials><AffiliationInfo><Affiliation>Center of Scientific Excellence for Influenza Viruses, Environmental Research Division, National Research Centre (NRC), Cairo 12622, Egypt. Mahmoud.Shehata@human-link.org.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Mostafa</LastName><ForeName>Ahmed</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Center of Scientific Excellence for Influenza Viruses, Environmental Research Division, National Research Centre (NRC), Cairo 12622, Egypt. ahmed_elsayed@daad-alumni.de.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Institute of Medical Virology, Justus-Liebig University Giessen, 35392 Giessen , Germany. ahmed_elsayed@daad-alumni.de.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Teubner</LastName><ForeName>Lisa</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Institute of Medical Microbiology, German Center for Infection Research (DZIF), Partner Site Giessen-Marburg-Langen Site, Justus-Liebig University Giessen, 35392 Giessen , Germany. lisa.teubner@mikrobio.med.uni-giessen.de.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Mahmoud</LastName><ForeName>Sara H</ForeName><Initials>SH</Initials><AffiliationInfo><Affiliation>Center of Scientific Excellence for Influenza Viruses, Environmental Research Division, National Research Centre (NRC), Cairo 12622, Egypt. sara.Hussein@human-link.org.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kandeil</LastName><ForeName>Ahmed</ForeName><Initials>A</Initials><Identifier Source="ORCID">0000-0003-3253-6961</Identifier><AffiliationInfo><Affiliation>Center of Scientific Excellence for Influenza Viruses, Environmental Research Division, National Research Centre (NRC), Cairo 12622, Egypt. ahmed.Kandeil@human-link.org.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Elshesheny</LastName><ForeName>Rabeh</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Center of Scientific Excellence for Influenza Viruses, Environmental Research Division, National Research Centre (NRC), Cairo 12622, Egypt. rabeh.elshesheny@stjude.org.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Frantz</LastName><ForeName>Renate</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Institute of Medical Microbiology, German Center for Infection Research (DZIF), Partner Site Giessen-Marburg-Langen Site, Justus-Liebig University Giessen, 35392 Giessen , Germany. renate.frantz@mikrobio.med.uni-giessen.de.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>La Pietra</LastName><ForeName>Luigi</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Institute of Medical Microbiology, German Center for Infection Research (DZIF), Partner Site Giessen-Marburg-Langen Site, Justus-Liebig University Giessen, 35392 Giessen , Germany. luigi.La-pietra@mikrobio.med.uni-giessen.de.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Pleschka</LastName><ForeName>Stephan</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Institute of Medical Virology, Justus-Liebig University Giessen, 35392 Giessen , Germany. Stephan.Pleschka@viro.med.uni-giessen.de.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Osman</LastName><ForeName>Ahmed</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Department of Biochemistry, Faculty of Science, Ain Shams University, Cairo 38105, Egypt. aoegiza@yahoo.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kayali</LastName><ForeName>Ghazi</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>Department of Epidemiology, Human Genetics, and Environmental Sciences, University of Texas, Houston, TX 77030, USA. ghazi@human-link.org.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Human Link, Baabda 1109, Lebanon. ghazi@human-link.org.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chakraborty</LastName><ForeName>Trinad</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Institute of Medical Microbiology, German Center for Infection Research (DZIF), Partner Site Giessen-Marburg-Langen Site, Justus-Liebig University Giessen, 35392 Giessen , Germany. trinad.chakraborty@mikrobio.med.uni-giessen.de.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ali</LastName><ForeName>Mohamed A</ForeName><Initials>MA</Initials><AffiliationInfo><Affiliation>Center of Scientific Excellence for Influenza Viruses, Environmental Research Division, National Research Centre (NRC), Cairo 12622, Egypt. Mobarak.Mraheil@mikrobio.med.uni-giessen.de.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Mraheil</LastName><ForeName>Mobarak Abu</ForeName><Initials>MA</Initials><AffiliationInfo><Affiliation>Institute of Medical Microbiology, German Center for Infection Research (DZIF), Partner Site Giessen-Marburg-Langen Site, Justus-Liebig University Giessen, 35392 Giessen , Germany. Mohamedahmedali2004@yahoo.com.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>HHSN272201400006C</GrantID><Agency>National Institute of Allergy and Infectious Diseases, National Institutes of Health, Department of Health and Human Services</Agency><Country>International</Country></Grant><Grant><GrantID>5175</GrantID><Agency>Science and Technology Development Fund (STDF) in Egypt</Agency><Country>International</Country></Grant><Grant><GrantID>T. C1</GrantID><Agency>DFG-funded Collaborative Research Centres 1021</Agency><Country>International</Country></Grant><Grant><GrantID>TP A4</GrantID><Agency>Transregional Collaborative Research Centre TRR 84</Agency><Country>International</Country></Grant><Grant><GrantID>TTU 01.803</GrantID><Agency>German Centre for Infection Research</Agency><Country>International</Country></Grant><Grant><GrantID>TT110801</GrantID><Agency>NRC-internal project</Agency><Country>International</Country></Grant><Grant><GrantID>PROANTILIS</GrantID><Agency>Bundesministerium für Bildung und Forschung</Agency><Country>International</Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2019</Year><Month>05</Month><Day>28</Day></ArticleDate></Article><MedlineJournalInfo><Country>Switzerland</Country><MedlineTA>Vaccines (Basel)</MedlineTA><NlmUniqueID>101629355</NlmUniqueID><ISSNLinking>2076-393X</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">H1N1pdm</Keyword><Keyword MajorTopicYN="Y">MERS-CoV</Keyword><Keyword MajorTopicYN="Y">OMVs</Keyword><Keyword MajorTopicYN="Y">influenza vaccine</Keyword></KeywordList><CoiStatement>The authors declare no conflict of interest.</CoiStatement></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>04</Month><Day>22</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2019</Year><Month>05</Month><Day>22</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2019</Year><Month>05</Month><Day>24</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2019</Year><Month>5</Month><Day>31</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate 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