Bacterial Outer Membrane Vesicles (OMVs)-based Dual Vaccine for Influenza A H1N1 Virus and MERS-CoV.
Identifieur interne : 000519 ( PubMed/Curation ); précédent : 000518; suivant : 000520Bacterial Outer Membrane Vesicles (OMVs)-based Dual Vaccine for Influenza A H1N1 Virus and MERS-CoV.
Auteurs : Mahmoud M. Shehata [Égypte] ; Ahmed Mostafa [Égypte] ; Lisa Teubner [Allemagne] ; Sara H. Mahmoud [Égypte] ; Ahmed Kandeil [Égypte] ; Rabeh Elshesheny [Égypte] ; Renate Frantz [Allemagne] ; Luigi La Pietra [Allemagne] ; Stephan Pleschka [Allemagne] ; Ahmed Osman [Égypte] ; Ghazi Kayali [États-Unis] ; Trinad Chakraborty [Allemagne] ; Mohamed A. Ali [Égypte] ; Mobarak Abu Mraheil [Allemagne]Source :
- Vaccines [ 2076-393X ] ; 2019.
Abstract
Vaccination is the most functional medical intervention to prophylactically control severe diseases caused by human-to-human or animal-to-human transmissible viral pathogens. Annually, seasonal influenza epidemics attack human populations leading to 290-650 thousand deaths/year worldwide. Recently, a novel Middle East Respiratory Syndrome Coronavirus emerged. Together, those two viruses present a significant public health burden in areas where they circulate. Herein, we generated a bacterial outer membrane vesicles (OMVs)-based vaccine presenting the antigenic stable chimeric fusion protein of the H1-type haemagglutinin (HA) of the pandemic influenza A virus (H1N1) strain from 2009 (H1N1pdm09) and the receptor binding domain (RBD) of the Middle East Respiratory Syndrome Coronavirus (MERS-CoV) (OMVs-H1/RBD). Our results showed that the chimeric antigen could induce specific neutralizing antibodies against both strains leading to protection of immunized mice against H1N1pdm09 and efficient neutralization of MERS-CoV. This study demonstrate that OMVs-based vaccines presenting viral antigens provide a safe and reliable approach to protect against two different viral infections.
DOI: 10.3390/vaccines7020046
PubMed: 31141982
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<author><name sortKey="Mostafa, Ahmed" sort="Mostafa, Ahmed" uniqKey="Mostafa A" first="Ahmed" last="Mostafa">Ahmed Mostafa</name>
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<author><name sortKey="Elshesheny, Rabeh" sort="Elshesheny, Rabeh" uniqKey="Elshesheny R" first="Rabeh" last="Elshesheny">Rabeh Elshesheny</name>
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<author><name sortKey="Frantz, Renate" sort="Frantz, Renate" uniqKey="Frantz R" first="Renate" last="Frantz">Renate Frantz</name>
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<author><name sortKey="La Pietra, Luigi" sort="La Pietra, Luigi" uniqKey="La Pietra L" first="Luigi" last="La Pietra">Luigi La Pietra</name>
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<country xml:lang="fr">Allemagne</country>
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<author><name sortKey="Pleschka, Stephan" sort="Pleschka, Stephan" uniqKey="Pleschka S" first="Stephan" last="Pleschka">Stephan Pleschka</name>
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<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Institute of Medical Virology, Justus-Liebig University Giessen, 35392 Giessen </wicri:regionArea>
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<author><name sortKey="Osman, Ahmed" sort="Osman, Ahmed" uniqKey="Osman A" first="Ahmed" last="Osman">Ahmed Osman</name>
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<author><name sortKey="Kayali, Ghazi" sort="Kayali, Ghazi" uniqKey="Kayali G" first="Ghazi" last="Kayali">Ghazi Kayali</name>
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<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Epidemiology, Human Genetics, and Environmental Sciences, University of Texas, Houston, TX 77030</wicri:regionArea>
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<author><name sortKey="Chakraborty, Trinad" sort="Chakraborty, Trinad" uniqKey="Chakraborty T" first="Trinad" last="Chakraborty">Trinad Chakraborty</name>
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<author><name sortKey="Ali, Mohamed A" sort="Ali, Mohamed A" uniqKey="Ali M" first="Mohamed A" last="Ali">Mohamed A. Ali</name>
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<country xml:lang="fr">Égypte</country>
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<author><name sortKey="Mraheil, Mobarak Abu" sort="Mraheil, Mobarak Abu" uniqKey="Mraheil M" first="Mobarak Abu" last="Mraheil">Mobarak Abu Mraheil</name>
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<front><div type="abstract" xml:lang="en">Vaccination is the most functional medical intervention to prophylactically control severe diseases caused by human-to-human or animal-to-human transmissible viral pathogens. Annually, seasonal influenza epidemics attack human populations leading to 290-650 thousand deaths/year worldwide. Recently, a novel Middle East Respiratory Syndrome Coronavirus emerged. Together, those two viruses present a significant public health burden in areas where they circulate. Herein, we generated a bacterial outer membrane vesicles (OMVs)-based vaccine presenting the antigenic stable chimeric fusion protein of the H1-type haemagglutinin (HA) of the pandemic influenza A virus (H1N1) strain from 2009 (H1N1pdm09) and the receptor binding domain (RBD) of the Middle East Respiratory Syndrome Coronavirus (MERS-CoV) (OMVs-H1/RBD). Our results showed that the chimeric antigen could induce specific neutralizing antibodies against both strains leading to protection of immunized mice against H1N1pdm09 and efficient neutralization of MERS-CoV. This study demonstrate that OMVs-based vaccines presenting viral antigens provide a safe and reliable approach to protect against two different viral infections.</div>
</front>
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<DateRevised><Year>2020</Year>
<Month>02</Month>
<Day>25</Day>
</DateRevised>
<Article PubModel="Electronic"><Journal><ISSN IssnType="Print">2076-393X</ISSN>
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<Issue>2</Issue>
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<Month>05</Month>
<Day>28</Day>
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<Title>Vaccines</Title>
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<ArticleTitle>Bacterial Outer Membrane Vesicles (OMVs)-based Dual Vaccine for Influenza A H1N1 Virus and MERS-CoV.</ArticleTitle>
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<Abstract><AbstractText>Vaccination is the most functional medical intervention to prophylactically control severe diseases caused by human-to-human or animal-to-human transmissible viral pathogens. Annually, seasonal influenza epidemics attack human populations leading to 290-650 thousand deaths/year worldwide. Recently, a novel Middle East Respiratory Syndrome Coronavirus emerged. Together, those two viruses present a significant public health burden in areas where they circulate. Herein, we generated a bacterial outer membrane vesicles (OMVs)-based vaccine presenting the antigenic stable chimeric fusion protein of the H1-type haemagglutinin (HA) of the pandemic influenza A virus (H1N1) strain from 2009 (H1N1pdm09) and the receptor binding domain (RBD) of the Middle East Respiratory Syndrome Coronavirus (MERS-CoV) (OMVs-H1/RBD). Our results showed that the chimeric antigen could induce specific neutralizing antibodies against both strains leading to protection of immunized mice against H1N1pdm09 and efficient neutralization of MERS-CoV. This study demonstrate that OMVs-based vaccines presenting viral antigens provide a safe and reliable approach to protect against two different viral infections.</AbstractText>
</Abstract>
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<ForeName>Mahmoud M</ForeName>
<Initials>MM</Initials>
<AffiliationInfo><Affiliation>Center of Scientific Excellence for Influenza Viruses, Environmental Research Division, National Research Centre (NRC), Cairo 12622, Egypt. Mahmoud.Shehata@human-link.org.</Affiliation>
</AffiliationInfo>
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<Author ValidYN="Y"><LastName>Mostafa</LastName>
<ForeName>Ahmed</ForeName>
<Initials>A</Initials>
<AffiliationInfo><Affiliation>Center of Scientific Excellence for Influenza Viruses, Environmental Research Division, National Research Centre (NRC), Cairo 12622, Egypt. ahmed_elsayed@daad-alumni.de.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Institute of Medical Virology, Justus-Liebig University Giessen, 35392 Giessen , Germany. ahmed_elsayed@daad-alumni.de.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Teubner</LastName>
<ForeName>Lisa</ForeName>
<Initials>L</Initials>
<AffiliationInfo><Affiliation>Institute of Medical Microbiology, German Center for Infection Research (DZIF), Partner Site Giessen-Marburg-Langen Site, Justus-Liebig University Giessen, 35392 Giessen , Germany. lisa.teubner@mikrobio.med.uni-giessen.de.</Affiliation>
</AffiliationInfo>
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<Author ValidYN="Y"><LastName>Mahmoud</LastName>
<ForeName>Sara H</ForeName>
<Initials>SH</Initials>
<AffiliationInfo><Affiliation>Center of Scientific Excellence for Influenza Viruses, Environmental Research Division, National Research Centre (NRC), Cairo 12622, Egypt. sara.Hussein@human-link.org.</Affiliation>
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<Author ValidYN="Y"><LastName>Kandeil</LastName>
<ForeName>Ahmed</ForeName>
<Initials>A</Initials>
<Identifier Source="ORCID">0000-0003-3253-6961</Identifier>
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</ArticleIdList>
<ReferenceList><Reference><Citation>J Appl Microbiol. 2001 Oct;91(4):572-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11576290</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Annu Rev Biochem. 2002;71:635-700</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12045108</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol Methods. 2009 Aug;159(2):244-50</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19406157</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nature. 2009 Jun 18;459(7249):931-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19525932</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>N Engl J Med. 2009 Dec 31;361(27):2628-36</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20042754</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>FEMS Immunol Med Microbiol. 2010 Oct;60(1):18-27</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20528929</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Trends Genet. 2010 Aug;26(8):345-52</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20594608</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2010 Aug 3;107(31):13701-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20615991</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Expert Rev Vaccines. 2011 May;10(5):559-61</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21604975</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Hum Vaccin. 2011 Aug;7(8):886-90</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21785281</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nature. 2013 Mar 14;495(7440):251-4</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23486063</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>BMJ. 2013 Apr 04;346:f2151</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23558347</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol Methods. 2013 Nov;193(2):603-10</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23886561</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Protein Cell. 2013 Nov;4(11):846-53</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24136722</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS One. 2013 Dec 04;8(12):e81587</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24324708</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Lancet. 2014 Feb 22;383(9918):714-21</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24507376</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biomed Res Int. 2014;2014:546274</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24982895</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Extracell Vesicles. 2014 Aug 11;3:null</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25147647</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Microb Cell Fact. 2015 Apr 16;14:57</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25890161</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Gen Virol. 2016 Mar;97(3):620-31</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26743314</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Front Med. 2016 Jun;10(2):120-36</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26791756</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Vaccine. 2016 Mar 4;34(10):1252-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26827663</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biotechnol J. 2015 Sep;10(11):1689-706</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26912077</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Immunol Res. 2016;2016:3576028</Citation>
<ArticleIdList><ArticleId IdType="pubmed">27239480</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Antiviral Res. 2017 Apr;140:55-61</Citation>
<ArticleIdList><ArticleId IdType="pubmed">28040513</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Ther. 2017 Apr 5;25(4):989-1002</Citation>
<ArticleIdList><ArticleId IdType="pubmed">28215994</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Hum Vaccin Immunother. 2017 Jul 3;13(7):1615-1624</Citation>
<ArticleIdList><ArticleId IdType="pubmed">28277821</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Euro Surveill. 2017 Mar 16;22(11):</Citation>
<ArticleIdList><ArticleId IdType="pubmed">28333616</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS One. 2017 Mar 27;12(3):e0174488</Citation>
<ArticleIdList><ArticleId IdType="pubmed">28346512</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biotechnol Adv. 2017 Sep;35(5):565-574</Citation>
<ArticleIdList><ArticleId IdType="pubmed">28522212</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biochem Biophys Res Commun. 2017 Aug 26;490(3):991-996</Citation>
<ArticleIdList><ArticleId IdType="pubmed">28666873</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Lancet Infect Dis. 2017 Nov;17(11):1133-1161</Citation>
<ArticleIdList><ArticleId IdType="pubmed">28843578</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Int J Nanomedicine. 2017 Sep 12;12:6813-6825</Citation>
<ArticleIdList><ArticleId IdType="pubmed">28979120</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS Pathog. 2017 Oct 23;13(10):e1006682</Citation>
<ArticleIdList><ArticleId IdType="pubmed">29059230</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2017 Nov 21;114(47):12578-12583</Citation>
<ArticleIdList><ArticleId IdType="pubmed">29109276</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Membr Biol. 2016 Sep - Dec;33(6-8):125-137</Citation>
<ArticleIdList><ArticleId IdType="pubmed">29189113</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Vaccine. 2018 Jan 4;36(2):322-330</Citation>
<ArticleIdList><ArticleId IdType="pubmed">29191736</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Trends Microbiol. 2018 Feb;26(2):87-89</Citation>
<ArticleIdList><ArticleId IdType="pubmed">29268980</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Vaccine. 2018 Feb 14;36(8):1047-1054</Citation>
<ArticleIdList><ArticleId IdType="pubmed">29406241</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Viruses. 2018 Feb 24;10(2):</Citation>
<ArticleIdList><ArticleId IdType="pubmed">29495250</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Vet Microbiol. 2018 Apr;217:104-111</Citation>
<ArticleIdList><ArticleId IdType="pubmed">29615242</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2018 Aug 29;92(18):</Citation>
<ArticleIdList><ArticleId IdType="pubmed">29950421</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Cell Rep. 2018 Jul 10;24(2):441-452</Citation>
<ArticleIdList><ArticleId IdType="pubmed">29996104</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Immunobiology. 2018 Dec;223(12):750-760</Citation>
<ArticleIdList><ArticleId IdType="pubmed">30055864</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>MBio. 2018 Oct 30;9(5):</Citation>
<ArticleIdList><ArticleId IdType="pubmed">30377284</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Front Immunol. 2018 Oct 29;9:2501</Citation>
<ArticleIdList><ArticleId IdType="pubmed">30459769</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Innate Immun. 2019;11(4):316-329</Citation>
<ArticleIdList><ArticleId IdType="pubmed">30844806</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
</PubmedData>
</pubmed>
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