MersV1, PubMed, Corpus, bibRecord, 002A70

Sequence-specific cleavage of RNA using chimeric DNA splints and RNase H.

Identifieur interne : 002A70 ( PubMed/Corpus ); précédent : 002A69; suivant : 002A71

Sequence-specific cleavage of RNA using chimeric DNA splints and RNase H.

Auteurs : H. Inoue ; Y. Hayase ; S. Iwai ; E. Ohtsuka

Source :

Nucleic acids symposium series [ 0261-3166 ] ; 1988.

RBID : pubmed:2465538

English descriptors

KwdEn :
- Base Sequence, Chimera, DNA, Endoribonucleases, Escherichia coli (enzymology), Molecular Sequence Data, Oligodeoxyribonucleotides, RNA, Ribonuclease H, Substrate Specificity.
MESH :
- chemical : DNA, Endoribonucleases, Oligodeoxyribonucleotides, RNA, Ribonuclease H.
- enzymology : Escherichia coli.
- Base Sequence, Chimera, Molecular Sequence Data, Substrate Specificity.

Abstract

To cleave RNA molecules using E. coli RNase H in a site-specific manner, a short oligodeoxyribonucleotide (3-5 mer) linked with oligo(2'-O-methyl)ribonucleotide(s) was designed to be used as a DNA splint. Our model experiments with ribooligomer the splint duplexes (9 mers) and RNase H demonstrated that a tetradeoxynucleotide cluster seems to be sufficient for the enzyme recognition and the short DNA-containing splint directs a unique cleavage of RNA by RNase H. The method could be applied to longer ribooligonucleotide substrates. For example, when 3'm (GA)d(AGAA)m(GGU)5' was used as a hybridization strand, 32pUCUUUCUUCUUCCAGGAU was cleaved specifically between U11 and C12 to yield 32pUCUUUCUUCUU. This method will have a variety of applications for the study of RNA.

PubMed: 2465538

Links to Exploration step

pubmed:2465538

Le document en format XML

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<author><name sortKey="Inoue, H" sort="Inoue, H" uniqKey="Inoue H" first="H" last="Inoue">H. Inoue</name>
<affiliation><nlm:affiliation>Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Hayase, Y" sort="Hayase, Y" uniqKey="Hayase Y" first="Y" last="Hayase">Y. Hayase</name>
</author>
<author><name sortKey="Iwai, S" sort="Iwai, S" uniqKey="Iwai S" first="S" last="Iwai">S. Iwai</name>
</author>
<author><name sortKey="Ohtsuka, E" sort="Ohtsuka, E" uniqKey="Ohtsuka E" first="E" last="Ohtsuka">E. Ohtsuka</name>
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<affiliation><nlm:affiliation>Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.</nlm:affiliation>
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<author><name sortKey="Hayase, Y" sort="Hayase, Y" uniqKey="Hayase Y" first="Y" last="Hayase">Y. Hayase</name>
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<author><name sortKey="Iwai, S" sort="Iwai, S" uniqKey="Iwai S" first="S" last="Iwai">S. Iwai</name>
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<author><name sortKey="Ohtsuka, E" sort="Ohtsuka, E" uniqKey="Ohtsuka E" first="E" last="Ohtsuka">E. Ohtsuka</name>
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<series><title level="j">Nucleic acids symposium series</title>
<idno type="ISSN">0261-3166</idno>
<imprint><date when="1988" type="published">1988</date>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Base Sequence</term>
<term>Chimera</term>
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<term>Endoribonucleases</term>
<term>Escherichia coli (enzymology)</term>
<term>Molecular Sequence Data</term>
<term>Oligodeoxyribonucleotides</term>
<term>RNA</term>
<term>Ribonuclease H</term>
<term>Substrate Specificity</term>
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<keywords scheme="MESH" type="chemical" xml:lang="en"><term>DNA</term>
<term>Endoribonucleases</term>
<term>Oligodeoxyribonucleotides</term>
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<term>Ribonuclease H</term>
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<keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Escherichia coli</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Base Sequence</term>
<term>Chimera</term>
<term>Molecular Sequence Data</term>
<term>Substrate Specificity</term>
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<front><div type="abstract" xml:lang="en">To cleave RNA molecules using E. coli RNase H in a site-specific manner, a short oligodeoxyribonucleotide (3-5 mer) linked with oligo(2'-O-methyl)ribonucleotide(s) was designed to be used as a DNA splint. Our model experiments with ribooligomer the splint duplexes (9 mers) and RNase H demonstrated that a tetradeoxynucleotide cluster seems to be sufficient for the enzyme recognition and the short DNA-containing splint directs a unique cleavage of RNA by RNase H. The method could be applied to longer ribooligonucleotide substrates. For example, when 3'm (GA)d(AGAA)m(GGU)5' was used as a hybridization strand, 32pUCUUUCUUCUUCCAGGAU was cleaved specifically between U11 and C12 to yield 32pUCUUUCUUCUU. This method will have a variety of applications for the study of RNA.</div>
</front>
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<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">2465538</PMID>
<DateCompleted><Year>1989</Year>
<Month>03</Month>
<Day>29</Day>
</DateCompleted>
<DateRevised><Year>2007</Year>
<Month>11</Month>
<Day>15</Day>
</DateRevised>
<Article PubModel="Print"><Journal><ISSN IssnType="Print">0261-3166</ISSN>
<JournalIssue CitedMedium="Print"><Issue>19</Issue>
<PubDate><Year>1988</Year>
</PubDate>
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<Title>Nucleic acids symposium series</Title>
<ISOAbbreviation>Nucleic Acids Symp. Ser.</ISOAbbreviation>
</Journal>
<ArticleTitle>Sequence-specific cleavage of RNA using chimeric DNA splints and RNase H.</ArticleTitle>
<Pagination><MedlinePgn>135-8</MedlinePgn>
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<Abstract><AbstractText>To cleave RNA molecules using E. coli RNase H in a site-specific manner, a short oligodeoxyribonucleotide (3-5 mer) linked with oligo(2'-O-methyl)ribonucleotide(s) was designed to be used as a DNA splint. Our model experiments with ribooligomer the splint duplexes (9 mers) and RNase H demonstrated that a tetradeoxynucleotide cluster seems to be sufficient for the enzyme recognition and the short DNA-containing splint directs a unique cleavage of RNA by RNase H. The method could be applied to longer ribooligonucleotide substrates. For example, when 3'm (GA)d(AGAA)m(GGU)5' was used as a hybridization strand, 32pUCUUUCUUCUUCCAGGAU was cleaved specifically between U11 and C12 to yield 32pUCUUUCUUCUU. This method will have a variety of applications for the study of RNA.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Inoue</LastName>
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<Language>eng</Language>
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<MedlineJournalInfo><Country>England</Country>
<MedlineTA>Nucleic Acids Symp Ser</MedlineTA>
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<ISSNLinking>0261-3166</ISSNLinking>
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<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D009838">Oligodeoxyribonucleotides</NameOfSubstance>
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</MeshHeading>
<MeshHeading><DescriptorName UI="D004247" MajorTopicYN="N">DNA</DescriptorName>
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<MeshHeading><DescriptorName UI="D004722" MajorTopicYN="Y">Endoribonucleases</DescriptorName>
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<MeshHeading><DescriptorName UI="D004926" MajorTopicYN="N">Escherichia coli</DescriptorName>
<QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName>
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<MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName>
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<MeshHeading><DescriptorName UI="D009838" MajorTopicYN="N">Oligodeoxyribonucleotides</DescriptorName>
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<MeshHeading><DescriptorName UI="D016914" MajorTopicYN="N">Ribonuclease H</DescriptorName>
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<MeshHeading><DescriptorName UI="D013379" MajorTopicYN="N">Substrate Specificity</DescriptorName>
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</record>

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Sequence-specific cleavage of RNA using chimeric DNA splints and RNase H.

Sequence-specific cleavage of RNA using chimeric DNA splints and RNase H.

Source :

English descriptors

Abstract

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki