MersV1, PubMed, Corpus, bibRecord, 002A46

Use of EDTA derivatization to characterize interactions between oligodeoxyribonucleoside methylphosphonates and nucleic acids.

Identifieur interne : 002A46 ( PubMed/Corpus ); précédent : 002A45; suivant : 002A47

Use of EDTA derivatization to characterize interactions between oligodeoxyribonucleoside methylphosphonates and nucleic acids.

Auteurs : S B Lin ; K R Blake ; P S Miller ; P O Ts'O

Source :

Biochemistry [ 0006-2960 ] ; 1989.

RBID : pubmed:2469462

English descriptors

KwdEn :
- Base Sequence, Chemical Phenomena, Chemistry, DNA, Edetic Acid, Indicators and Reagents, Nucleic Acid Denaturation, Oligodeoxyribonucleotides (chemical synthesis), Organophosphorus Compounds, Poly T, Poly U, Polydeoxyribonucleotides, RNA, Thermodynamics.
MESH :
- chemical , chemical synthesis : Oligodeoxyribonucleotides.
- chemical : DNA, Edetic Acid, Indicators and Reagents, Organophosphorus Compounds, Poly T, Poly U, Polydeoxyribonucleotides, RNA.
- Base Sequence, Chemical Phenomena, Chemistry, Nucleic Acid Denaturation, Thermodynamics.

Abstract

EDTA-derivatized oligonucleoside methylphosphonates were prepared and used to characterize hybridization between the oligomers and single-stranded DNA or RNA. The melting temperatures of duplexes formed between an oligodeoxyribonucleotide 35-mer and complementary methylphosphonate 12-mers were 4-12 degrees C higher than those of duplexes formed by oligodeoxyribonucleotide 12-mers as determined by spectrophotometric measurements. Derivatization of the methylphosphonate oligomers with EDTA reduced the melting temperature by 5 degrees C. Methylphosphonate oligomer-nucleic acid complexes were stabilized by base stacking interactions between the terminal bases of the two oligomers binding to adjacent binding sites on the target. In the presence of Fe2+ and DTT, the EDTA-derivatized oligomers produce hydroxyl radicals that cause degradation of the sugar-phosphate backbone of both targeted DNA and RNA. Degradation occurs specifically in the region of the oligomer binding site and is approximately 20-fold more efficient for single-stranded DNA than for RNA. In comparison to the presence of one oligomer, the extent of target degradation was increased considerably by additions of two oligomers that bind at adjacent sites on the target. For example, the extent of degradation of a single-stranded DNA 35-mer caused by two contiguously binding oligomers, one of which was derivatized by EDTA, was approximately 2 times greater than that caused by the EDTA-derivatized oligomer alone. Although EDTA-derivatized oligomers are stable for long periods of time in aqueous solution, they undergo rapid autodegradation in the presence of Fe2+ and DTT with half-lives of approximately 30 min. This autodegradation reaction renders the EDTA-derivatized oligomers unable to cause degradation of their complementary target nucleic acids.(ABSTRACT TRUNCATED AT 250 WORDS)

DOI: 10.1021/bi00429a020
PubMed: 2469462

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pubmed:2469462

Le document en format XML

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<author><name sortKey="Lin, S B" sort="Lin, S B" uniqKey="Lin S" first="S B" last="Lin">S B Lin</name>
<affiliation><nlm:affiliation>Division of Biophysics, School of Hygiene and Public Health, Johns Hopkins University, Baltimore, Maryland 21205.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Blake, K R" sort="Blake, K R" uniqKey="Blake K" first="K R" last="Blake">K R Blake</name>
</author>
<author><name sortKey="Miller, P S" sort="Miller, P S" uniqKey="Miller P" first="P S" last="Miller">P S Miller</name>
</author>
<author><name sortKey="Ts O, P O" sort="Ts O, P O" uniqKey="Ts O P" first="P O" last="Ts'O">P O Ts'O</name>
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<author><name sortKey="Miller, P S" sort="Miller, P S" uniqKey="Miller P" first="P S" last="Miller">P S Miller</name>
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<term>Edetic Acid</term>
<term>Indicators and Reagents</term>
<term>Nucleic Acid Denaturation</term>
<term>Oligodeoxyribonucleotides (chemical synthesis)</term>
<term>Organophosphorus Compounds</term>
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<term>Edetic Acid</term>
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<front><div type="abstract" xml:lang="en">EDTA-derivatized oligonucleoside methylphosphonates were prepared and used to characterize hybridization between the oligomers and single-stranded DNA or RNA. The melting temperatures of duplexes formed between an oligodeoxyribonucleotide 35-mer and complementary methylphosphonate 12-mers were 4-12 degrees C higher than those of duplexes formed by oligodeoxyribonucleotide 12-mers as determined by spectrophotometric measurements. Derivatization of the methylphosphonate oligomers with EDTA reduced the melting temperature by 5 degrees C. Methylphosphonate oligomer-nucleic acid complexes were stabilized by base stacking interactions between the terminal bases of the two oligomers binding to adjacent binding sites on the target. In the presence of Fe2+ and DTT, the EDTA-derivatized oligomers produce hydroxyl radicals that cause degradation of the sugar-phosphate backbone of both targeted DNA and RNA. Degradation occurs specifically in the region of the oligomer binding site and is approximately 20-fold more efficient for single-stranded DNA than for RNA. In comparison to the presence of one oligomer, the extent of target degradation was increased considerably by additions of two oligomers that bind at adjacent sites on the target. For example, the extent of degradation of a single-stranded DNA 35-mer caused by two contiguously binding oligomers, one of which was derivatized by EDTA, was approximately 2 times greater than that caused by the EDTA-derivatized oligomer alone. Although EDTA-derivatized oligomers are stable for long periods of time in aqueous solution, they undergo rapid autodegradation in the presence of Fe2+ and DTT with half-lives of approximately 30 min. This autodegradation reaction renders the EDTA-derivatized oligomers unable to cause degradation of their complementary target nucleic acids.(ABSTRACT TRUNCATED AT 250 WORDS)</div>
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<DateRevised><Year>2019</Year>
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<Abstract><AbstractText>EDTA-derivatized oligonucleoside methylphosphonates were prepared and used to characterize hybridization between the oligomers and single-stranded DNA or RNA. The melting temperatures of duplexes formed between an oligodeoxyribonucleotide 35-mer and complementary methylphosphonate 12-mers were 4-12 degrees C higher than those of duplexes formed by oligodeoxyribonucleotide 12-mers as determined by spectrophotometric measurements. Derivatization of the methylphosphonate oligomers with EDTA reduced the melting temperature by 5 degrees C. Methylphosphonate oligomer-nucleic acid complexes were stabilized by base stacking interactions between the terminal bases of the two oligomers binding to adjacent binding sites on the target. In the presence of Fe2+ and DTT, the EDTA-derivatized oligomers produce hydroxyl radicals that cause degradation of the sugar-phosphate backbone of both targeted DNA and RNA. Degradation occurs specifically in the region of the oligomer binding site and is approximately 20-fold more efficient for single-stranded DNA than for RNA. In comparison to the presence of one oligomer, the extent of target degradation was increased considerably by additions of two oligomers that bind at adjacent sites on the target. For example, the extent of degradation of a single-stranded DNA 35-mer caused by two contiguously binding oligomers, one of which was derivatized by EDTA, was approximately 2 times greater than that caused by the EDTA-derivatized oligomer alone. Although EDTA-derivatized oligomers are stable for long periods of time in aqueous solution, they undergo rapid autodegradation in the presence of Fe2+ and DTT with half-lives of approximately 30 min. This autodegradation reaction renders the EDTA-derivatized oligomers unable to cause degradation of their complementary target nucleic acids.(ABSTRACT TRUNCATED AT 250 WORDS)</AbstractText>
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Use of EDTA derivatization to characterize interactions between oligodeoxyribonucleoside methylphosphonates and nucleic acids.

Use of EDTA derivatization to characterize interactions between oligodeoxyribonucleoside methylphosphonates and nucleic acids.

Source :

English descriptors

Abstract

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

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