Inhibition of expression of a mouse alpha-globin gene by plasmids that include antisense oligonucleotides.
Identifieur interne : 002938 ( PubMed/Corpus ); précédent : 002937; suivant : 002939Inhibition of expression of a mouse alpha-globin gene by plasmids that include antisense oligonucleotides.
Auteurs : K. Yokoyama ; D X Hou ; H. Gao ; X. Tang ; I. Kitabayashi ; K. Nishikura ; G. GachelinSource :
- Cell structure and function [ 0386-7196 ] ; 1992.
English descriptors
- KwdEn :
- Acetamides (pharmacology), Animals, Base Sequence, Cell Differentiation (drug effects), DNA, Recombinant (genetics), Gene Expression Regulation (drug effects), Genes, myc, Globins (biosynthesis), Globins (genetics), Leukemia, Erythroblastic, Acute, Mice, Models, Genetic, Molecular Sequence Data, Plasmids, RNA Caps (genetics), RNA, Antisense (pharmacology), RNA, Messenger (biosynthesis), RNA, Messenger (genetics), Regulatory Sequences, Nucleic Acid, Tumor Cells, Cultured.
- MESH :
- chemical , biosynthesis : Globins, RNA, Messenger.
- chemical , genetics : DNA, Recombinant, Globins, RNA Caps, RNA, Messenger.
- chemical , pharmacology : Acetamides, RNA, Antisense.
- drug effects : Cell Differentiation, Gene Expression Regulation.
- Animals, Base Sequence, Genes, myc, Leukemia, Erythroblastic, Acute, Mice, Models, Genetic, Molecular Sequence Data, Plasmids, Regulatory Sequences, Nucleic Acid, Tumor Cells, Cultured.
Abstract
Plasmid-borne DNAs, corresponding to 68-base oligodeoxynucleotides, synthesized in the antisense or sense configuration and based on the nucleotide sequences of various regions of the mouse alpha-globin mRNA, were introduced with the gene for xanthine-guanine phosphoribosyl transferase from E. coli (Ecogpt) into mouse erythroleukemia (MEL) cells by protoplast fusion. Specific inhibition of the synthesis of alpha-globin was observed only in the cells transformed with the plasmids with antisense 68-mers that corresponded to the cap site as well as the site of initiation of translation of alpha-globin mRNA (Oligo-A); Other plasmids with antisense 68-mers that corresponded to the regions of the exon/intron junctions, the individual exons, or the 3' untranslated region were ineffective. This antisense RNA efficiently reduced the production of alpha-globin to 9-18% of the endogenous level after induction with hexylmethylene-bis-acetoamide (HMBA). Moreover, most of the antisense transformants did not show any decrease in the expression of the c-myc gene at the early phases of differentiation of MEL cells. Thus, we propose a hypothesis that the early decline in levels of c-myc mRNA may be independent of and uncoupled from the program of globin synthesis during the differentiation of MEL cells.
DOI: 10.1247/csf.17.433
PubMed: 1295698
Links to Exploration step
pubmed:1295698Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Inhibition of expression of a mouse alpha-globin gene by plasmids that include antisense oligonucleotides.</title><author><name sortKey="Yokoyama, K" sort="Yokoyama, K" uniqKey="Yokoyama K" first="K" last="Yokoyama">K. Yokoyama</name><affiliation><nlm:affiliation>Gene Bank, Tsukuba Life Science Center, RIKEN (The Institute of Physical and Chemical Research), Ibaraki, Japan.</nlm:affiliation></affiliation></author><author><name sortKey="Hou, D X" sort="Hou, D X" uniqKey="Hou D" first="D X" last="Hou">D X Hou</name></author><author><name sortKey="Gao, H" sort="Gao, H" uniqKey="Gao H" first="H" last="Gao">H. Gao</name></author><author><name sortKey="Tang, X" sort="Tang, X" uniqKey="Tang X" first="X" last="Tang">X. Tang</name></author><author><name sortKey="Kitabayashi, I" sort="Kitabayashi, I" uniqKey="Kitabayashi I" first="I" last="Kitabayashi">I. Kitabayashi</name></author><author><name sortKey="Nishikura, K" sort="Nishikura, K" uniqKey="Nishikura K" first="K" last="Nishikura">K. Nishikura</name></author><author><name sortKey="Gachelin, G" sort="Gachelin, G" uniqKey="Gachelin G" first="G" last="Gachelin">G. Gachelin</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1992">1992</date><idno type="RBID">pubmed:1295698</idno><idno type="pmid">1295698</idno><idno type="doi">10.1247/csf.17.433</idno><idno type="wicri:Area/PubMed/Corpus">002938</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002938</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Inhibition of expression of a mouse alpha-globin gene by plasmids that include antisense oligonucleotides.</title><author><name sortKey="Yokoyama, K" sort="Yokoyama, K" uniqKey="Yokoyama K" first="K" last="Yokoyama">K. Yokoyama</name><affiliation><nlm:affiliation>Gene Bank, Tsukuba Life Science Center, RIKEN (The Institute of Physical and Chemical Research), Ibaraki, Japan.</nlm:affiliation></affiliation></author><author><name sortKey="Hou, D X" sort="Hou, D X" uniqKey="Hou D" first="D X" last="Hou">D X Hou</name></author><author><name sortKey="Gao, H" sort="Gao, H" uniqKey="Gao H" first="H" last="Gao">H. Gao</name></author><author><name sortKey="Tang, X" sort="Tang, X" uniqKey="Tang X" first="X" last="Tang">X. Tang</name></author><author><name sortKey="Kitabayashi, I" sort="Kitabayashi, I" uniqKey="Kitabayashi I" first="I" last="Kitabayashi">I. Kitabayashi</name></author><author><name sortKey="Nishikura, K" sort="Nishikura, K" uniqKey="Nishikura K" first="K" last="Nishikura">K. Nishikura</name></author><author><name sortKey="Gachelin, G" sort="Gachelin, G" uniqKey="Gachelin G" first="G" last="Gachelin">G. Gachelin</name></author></analytic><series><title level="j">Cell structure and function</title><idno type="ISSN">0386-7196</idno><imprint><date when="1992" type="published">1992</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Acetamides (pharmacology)</term><term>Animals</term><term>Base Sequence</term><term>Cell Differentiation (drug effects)</term><term>DNA, Recombinant (genetics)</term><term>Gene Expression Regulation (drug effects)</term><term>Genes, myc</term><term>Globins (biosynthesis)</term><term>Globins (genetics)</term><term>Leukemia, Erythroblastic, Acute</term><term>Mice</term><term>Models, Genetic</term><term>Molecular Sequence Data</term><term>Plasmids</term><term>RNA Caps (genetics)</term><term>RNA, Antisense (pharmacology)</term><term>RNA, Messenger (biosynthesis)</term><term>RNA, Messenger (genetics)</term><term>Regulatory Sequences, Nucleic Acid</term><term>Tumor Cells, Cultured</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Globins</term><term>RNA, Messenger</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Recombinant</term><term>Globins</term><term>RNA Caps</term><term>RNA, Messenger</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Acetamides</term><term>RNA, Antisense</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Cell Differentiation</term><term>Gene Expression Regulation</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Base Sequence</term><term>Genes, myc</term><term>Leukemia, Erythroblastic, Acute</term><term>Mice</term><term>Models, Genetic</term><term>Molecular Sequence Data</term><term>Plasmids</term><term>Regulatory Sequences, Nucleic Acid</term><term>Tumor Cells, Cultured</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Plasmid-borne DNAs, corresponding to 68-base oligodeoxynucleotides, synthesized in the antisense or sense configuration and based on the nucleotide sequences of various regions of the mouse alpha-globin mRNA, were introduced with the gene for xanthine-guanine phosphoribosyl transferase from E. coli (Ecogpt) into mouse erythroleukemia (MEL) cells by protoplast fusion. Specific inhibition of the synthesis of alpha-globin was observed only in the cells transformed with the plasmids with antisense 68-mers that corresponded to the cap site as well as the site of initiation of translation of alpha-globin mRNA (Oligo-A); Other plasmids with antisense 68-mers that corresponded to the regions of the exon/intron junctions, the individual exons, or the 3' untranslated region were ineffective. This antisense RNA efficiently reduced the production of alpha-globin to 9-18% of the endogenous level after induction with hexylmethylene-bis-acetoamide (HMBA). Moreover, most of the antisense transformants did not show any decrease in the expression of the c-myc gene at the early phases of differentiation of MEL cells. Thus, we propose a hypothesis that the early decline in levels of c-myc mRNA may be independent of and uncoupled from the program of globin synthesis during the differentiation of MEL cells.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">1295698</PMID><DateCompleted><Year>1993</Year><Month>04</Month><Day>20</Day></DateCompleted><DateRevised><Year>2019</Year><Month>09</Month><Day>18</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0386-7196</ISSN><JournalIssue CitedMedium="Print"><Volume>17</Volume><Issue>6</Issue><PubDate><Year>1992</Year><Month>Dec</Month></PubDate></JournalIssue><Title>Cell structure and function</Title><ISOAbbreviation>Cell Struct. Funct.</ISOAbbreviation></Journal><ArticleTitle>Inhibition of expression of a mouse alpha-globin gene by plasmids that include antisense oligonucleotides.</ArticleTitle><Pagination><MedlinePgn>433-42</MedlinePgn></Pagination><Abstract><AbstractText>Plasmid-borne DNAs, corresponding to 68-base oligodeoxynucleotides, synthesized in the antisense or sense configuration and based on the nucleotide sequences of various regions of the mouse alpha-globin mRNA, were introduced with the gene for xanthine-guanine phosphoribosyl transferase from E. coli (Ecogpt) into mouse erythroleukemia (MEL) cells by protoplast fusion. Specific inhibition of the synthesis of alpha-globin was observed only in the cells transformed with the plasmids with antisense 68-mers that corresponded to the cap site as well as the site of initiation of translation of alpha-globin mRNA (Oligo-A); Other plasmids with antisense 68-mers that corresponded to the regions of the exon/intron junctions, the individual exons, or the 3' untranslated region were ineffective. This antisense RNA efficiently reduced the production of alpha-globin to 9-18% of the endogenous level after induction with hexylmethylene-bis-acetoamide (HMBA). Moreover, most of the antisense transformants did not show any decrease in the expression of the c-myc gene at the early phases of differentiation of MEL cells. Thus, we propose a hypothesis that the early decline in levels of c-myc mRNA may be independent of and uncoupled from the program of globin synthesis during the differentiation of MEL cells.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Yokoyama</LastName><ForeName>K</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>Gene Bank, Tsukuba Life Science Center, RIKEN (The Institute of Physical and Chemical Research), Ibaraki, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hou</LastName><ForeName>D X</ForeName><Initials>DX</Initials></Author><Author ValidYN="Y"><LastName>Gao</LastName><ForeName>H</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Tang</LastName><ForeName>X</ForeName><Initials>X</Initials></Author><Author ValidYN="Y"><LastName>Kitabayashi</LastName><ForeName>I</ForeName><Initials>I</Initials></Author><Author ValidYN="Y"><LastName>Nishikura</LastName><ForeName>K</ForeName><Initials>K</Initials></Author><Author ValidYN="Y"><LastName>Gachelin</LastName><ForeName>G</ForeName><Initials>G</Initials></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>CA 46676</GrantID><Acronym>CA</Acronym><Agency>NCI NIH HHS</Agency><Country>United States</Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType><PublicationType UI="D013487">Research Support, U.S. Gov't, P.H.S.</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Japan</Country><MedlineTA>Cell Struct Funct</MedlineTA><NlmUniqueID>7608465</NlmUniqueID><ISSNLinking>0386-7196</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance 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MajorTopicYN="N">Acetamides</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001483" MajorTopicYN="N">Base Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002454" MajorTopicYN="N">Cell Differentiation</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004274" MajorTopicYN="N">DNA, Recombinant</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005786" MajorTopicYN="Y">Gene Expression Regulation</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016259" MajorTopicYN="N">Genes, 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