Single base discrimination of CENP-B repeats on mouse and human Chromosomes with PNA-FISH.
Identifieur interne : 002653 ( PubMed/Corpus ); précédent : 002652; suivant : 002654Single base discrimination of CENP-B repeats on mouse and human Chromosomes with PNA-FISH.
Auteurs : C. Chen ; Y K Hong ; S D Ontiveros ; M. Egholm ; W M StraussSource :
- Mammalian genome : official journal of the International Mammalian Genome Society [ 0938-8990 ] ; 1999.
English descriptors
- KwdEn :
- Animals, Autoantigens, Base Pairing, Centromere Protein B, Chromosomal Proteins, Non-Histone (genetics), DNA Primers, DNA-Binding Proteins, Humans, In Situ Hybridization, Fluorescence (methods), Mice, Mice, Inbred C57BL, Peptide Nucleic Acids, Repetitive Sequences, Nucleic Acid, Sensitivity and Specificity, Species Specificity.
- MESH :
- chemical , genetics : Chromosomal Proteins, Non-Histone.
- chemical : Autoantigens, Centromere Protein B, DNA Primers, DNA-Binding Proteins, Peptide Nucleic Acids.
- methods : In Situ Hybridization, Fluorescence.
- Animals, Base Pairing, Humans, Mice, Mice, Inbred C57BL, Repetitive Sequences, Nucleic Acid, Sensitivity and Specificity, Species Specificity.
Abstract
The satellite repeat structure of the mammalian centromere contains the CENP-B protein binding site. Using the peptide nucleic acid-fluorescence in situ hybridization (PNA-FISH), we show by direct PNA-DNA binding that all detectable CENP-B sites in a mammalian genome might have the same sequence. Two species-specific PNA 17-mers, pMm and pMc, were identified from CENP-B binding sites of Mus musculus and M. caroli, respectively. Fluorescence in situ hybridization confirmed that pMc hybridized to M. caroli centromeres only; however, pMm cross-hybridized to M. musculus and human centromeres. By using a series of CENP-B PNA 17-mers containing 1, 2, 3, 5, and 7 base-pair mismatches to their DNA counterparts, we further demonstrate that PNA-FISH can discriminate between two CENP-B DNA sequences that differ by a single base-pair in mouse and human centromeres, suggesting the degree of conservation of CENP-B sequences throughout the genome. In comparison with DNA oligonucleotides, PNA oligomers demonstrate the higher sequence specificity, improved stability, reproducibility, and lower background. Therefore, PNA oligomers have significant advantages over DNA oligonucleotide probes in analyzing microsatellites in a genome.
DOI: 10.1007/s003359900934
PubMed: 9892726
Links to Exploration step
pubmed:9892726Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Single base discrimination of CENP-B repeats on mouse and human Chromosomes with PNA-FISH.</title><author><name sortKey="Chen, C" sort="Chen, C" uniqKey="Chen C" first="C" last="Chen">C. Chen</name><affiliation><nlm:affiliation>Harvard Institutes of Human Genetics, Harvard Medical School, Beth Israel Deaconess Medical Center, 4 Blackfan Circle, Boston, Massachusetts 02115, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Hong, Y K" sort="Hong, Y K" uniqKey="Hong Y" first="Y K" last="Hong">Y K Hong</name></author><author><name sortKey="Ontiveros, S D" sort="Ontiveros, S D" uniqKey="Ontiveros S" first="S D" last="Ontiveros">S D Ontiveros</name></author><author><name sortKey="Egholm, M" sort="Egholm, M" uniqKey="Egholm M" first="M" last="Egholm">M. Egholm</name></author><author><name sortKey="Strauss, W M" sort="Strauss, W M" uniqKey="Strauss W" first="W M" last="Strauss">W M Strauss</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1999">1999</date><idno type="RBID">pubmed:9892726</idno><idno type="pmid">9892726</idno><idno type="doi">10.1007/s003359900934</idno><idno type="wicri:Area/PubMed/Corpus">002653</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002653</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Single base discrimination of CENP-B repeats on mouse and human Chromosomes with PNA-FISH.</title><author><name sortKey="Chen, C" sort="Chen, C" uniqKey="Chen C" first="C" last="Chen">C. Chen</name><affiliation><nlm:affiliation>Harvard Institutes of Human Genetics, Harvard Medical School, Beth Israel Deaconess Medical Center, 4 Blackfan Circle, Boston, Massachusetts 02115, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Hong, Y K" sort="Hong, Y K" uniqKey="Hong Y" first="Y K" last="Hong">Y K Hong</name></author><author><name sortKey="Ontiveros, S D" sort="Ontiveros, S D" uniqKey="Ontiveros S" first="S D" last="Ontiveros">S D Ontiveros</name></author><author><name sortKey="Egholm, M" sort="Egholm, M" uniqKey="Egholm M" first="M" last="Egholm">M. Egholm</name></author><author><name sortKey="Strauss, W M" sort="Strauss, W M" uniqKey="Strauss W" first="W M" last="Strauss">W M Strauss</name></author></analytic><series><title level="j">Mammalian genome : official journal of the International Mammalian Genome Society</title><idno type="ISSN">0938-8990</idno><imprint><date when="1999" type="published">1999</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Autoantigens</term><term>Base Pairing</term><term>Centromere Protein B</term><term>Chromosomal Proteins, Non-Histone (genetics)</term><term>DNA Primers</term><term>DNA-Binding Proteins</term><term>Humans</term><term>In Situ Hybridization, Fluorescence (methods)</term><term>Mice</term><term>Mice, Inbred C57BL</term><term>Peptide Nucleic Acids</term><term>Repetitive Sequences, Nucleic Acid</term><term>Sensitivity and Specificity</term><term>Species Specificity</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Chromosomal Proteins, Non-Histone</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Autoantigens</term><term>Centromere Protein B</term><term>DNA Primers</term><term>DNA-Binding Proteins</term><term>Peptide Nucleic Acids</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>In Situ Hybridization, Fluorescence</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Base Pairing</term><term>Humans</term><term>Mice</term><term>Mice, Inbred C57BL</term><term>Repetitive Sequences, Nucleic Acid</term><term>Sensitivity and Specificity</term><term>Species Specificity</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The satellite repeat structure of the mammalian centromere contains the CENP-B protein binding site. Using the peptide nucleic acid-fluorescence in situ hybridization (PNA-FISH), we show by direct PNA-DNA binding that all detectable CENP-B sites in a mammalian genome might have the same sequence. Two species-specific PNA 17-mers, pMm and pMc, were identified from CENP-B binding sites of Mus musculus and M. caroli, respectively. Fluorescence in situ hybridization confirmed that pMc hybridized to M. caroli centromeres only; however, pMm cross-hybridized to M. musculus and human centromeres. By using a series of CENP-B PNA 17-mers containing 1, 2, 3, 5, and 7 base-pair mismatches to their DNA counterparts, we further demonstrate that PNA-FISH can discriminate between two CENP-B DNA sequences that differ by a single base-pair in mouse and human centromeres, suggesting the degree of conservation of CENP-B sequences throughout the genome. In comparison with DNA oligonucleotides, PNA oligomers demonstrate the higher sequence specificity, improved stability, reproducibility, and lower background. Therefore, PNA oligomers have significant advantages over DNA oligonucleotide probes in analyzing microsatellites in a genome.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">9892726</PMID><DateCompleted><Year>1999</Year><Month>03</Month><Day>01</Day></DateCompleted><DateRevised><Year>2019</Year><Month>09</Month><Day>05</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0938-8990</ISSN><JournalIssue CitedMedium="Print"><Volume>10</Volume><Issue>1</Issue><PubDate><Year>1999</Year><Month>Jan</Month></PubDate></JournalIssue><Title>Mammalian genome : official journal of the International Mammalian Genome Society</Title><ISOAbbreviation>Mamm. Genome</ISOAbbreviation></Journal><ArticleTitle>Single base discrimination of CENP-B repeats on mouse and human Chromosomes with PNA-FISH.</ArticleTitle><Pagination><MedlinePgn>13-8</MedlinePgn></Pagination><Abstract><AbstractText>The satellite repeat structure of the mammalian centromere contains the CENP-B protein binding site. Using the peptide nucleic acid-fluorescence in situ hybridization (PNA-FISH), we show by direct PNA-DNA binding that all detectable CENP-B sites in a mammalian genome might have the same sequence. Two species-specific PNA 17-mers, pMm and pMc, were identified from CENP-B binding sites of Mus musculus and M. caroli, respectively. Fluorescence in situ hybridization confirmed that pMc hybridized to M. caroli centromeres only; however, pMm cross-hybridized to M. musculus and human centromeres. By using a series of CENP-B PNA 17-mers containing 1, 2, 3, 5, and 7 base-pair mismatches to their DNA counterparts, we further demonstrate that PNA-FISH can discriminate between two CENP-B DNA sequences that differ by a single base-pair in mouse and human centromeres, suggesting the degree of conservation of CENP-B sequences throughout the genome. In comparison with DNA oligonucleotides, PNA oligomers demonstrate the higher sequence specificity, improved stability, reproducibility, and lower background. Therefore, PNA oligomers have significant advantages over DNA oligonucleotide probes in analyzing microsatellites in a genome.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>C</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Harvard Institutes of Human Genetics, Harvard Medical School, Beth Israel Deaconess Medical Center, 4 Blackfan Circle, Boston, Massachusetts 02115, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hong</LastName><ForeName>Y K</ForeName><Initials>YK</Initials></Author><Author ValidYN="Y"><LastName>Ontiveros</LastName><ForeName>S D</ForeName><Initials>SD</Initials></Author><Author ValidYN="Y"><LastName>Egholm</LastName><ForeName>M</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Strauss</LastName><ForeName>W M</ForeName><Initials>WM</Initials></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>1 P30 AG13314-01</GrantID><Acronym>AG</Acronym><Agency>NIA NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>R03 AG144176-01</GrantID><Acronym>AG</Acronym><Agency>NIA NIH HHS</Agency><Country>United States</Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType><PublicationType UI="D013487">Research Support, U.S. Gov't, P.H.S.</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Mamm Genome</MedlineTA><NlmUniqueID>9100916</NlmUniqueID><ISSNLinking>0938-8990</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D001324">Autoantigens</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C497926">CENPB protein, human</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C497927">Cenpb protein, mouse</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D051936">Centromere Protein B</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D002868">Chromosomal Proteins, Non-Histone</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D017931">DNA Primers</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004268">DNA-Binding Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D020135">Peptide Nucleic Acids</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001324" MajorTopicYN="Y">Autoantigens</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D020029" MajorTopicYN="N">Base Pairing</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D051936" MajorTopicYN="N">Centromere Protein B</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002868" MajorTopicYN="N">Chromosomal Proteins, Non-Histone</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017931" MajorTopicYN="N">DNA Primers</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004268" MajorTopicYN="Y">DNA-Binding Proteins</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017404" MajorTopicYN="N">In Situ Hybridization, Fluorescence</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D051379" MajorTopicYN="N">Mice</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008810" MajorTopicYN="N">Mice, Inbred C57BL</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D020135" MajorTopicYN="Y">Peptide Nucleic Acids</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012091" MajorTopicYN="Y">Repetitive Sequences, Nucleic Acid</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012680" MajorTopicYN="N">Sensitivity and Specificity</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013045" MajorTopicYN="N">Species Specificity</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1999</Year><Month>1</Month><Day>20</Day></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>1999</Year><Month>1</Month><Day>20</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate 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