MersV1, PubMed, Corpus, bibRecord, 001687

Engineering infectious cDNAs of coronavirus as bacterial artificial chromosomes.

Identifieur interne : 001687 ( PubMed/Corpus ); précédent : 001686; suivant : 001688

Engineering infectious cDNAs of coronavirus as bacterial artificial chromosomes.

Auteurs : Fernando Almazán ; Silvia Márquez-Jurado ; Aitor Nogales ; Luis Enjuanes

Source :

Methods in molecular biology (Clifton, N.J.) [ 1940-6029 ] ; 2015.

RBID : pubmed:25720478

English descriptors

KwdEn :
- Animals, Cell Line, Chromosomes, Artificial, Bacterial (genetics), Coronavirus (genetics), Cricetinae, DNA, Complementary (genetics), Escherichia coli, Genetic Engineering, Humans, Plasmids (genetics), Plasmids (isolation & purification), Reverse Genetics, Transformation, Genetic.
MESH :
- chemical , genetics : DNA, Complementary.
- genetics : Chromosomes, Artificial, Bacterial, Coronavirus, Plasmids.
- isolation & purification : Plasmids.
- Animals, Cell Line, Cricetinae, Escherichia coli, Genetic Engineering, Humans, Reverse Genetics, Transformation, Genetic.

Abstract

The large size of the coronavirus (CoV) genome (around 30 kb) and the instability in bacteria of plasmids carrying CoV replicase sequences represent serious restrictions for the development of CoV infectious clones using reverse genetic systems similar to those used for smaller positive sense RNA viruses. To overcome these problems, several approaches have been established in the last 13 years. Here we describe the engineering of CoV full-length cDNA clones as bacterial artificial chromosomes (BACs), using the Middle East respiratory syndrome CoV (MERS-CoV) as a model.

DOI: 10.1007/978-1-4939-2438-7_13
PubMed: 25720478

Links to Exploration step

pubmed:25720478

Le document en format XML

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<author><name sortKey="Almazan, Fernando" sort="Almazan, Fernando" uniqKey="Almazan F" first="Fernando" last="Almazán">Fernando Almazán</name>
<affiliation><nlm:affiliation>Department of Molecular and Cell Biology, Centro Nacional de Biotecnología (CNB-CSIC), Campus Universidad Autónoma de Madrid, Darwin 3, Cantoblanco, Madrid, 28049, Spain.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Marquez Jurado, Silvia" sort="Marquez Jurado, Silvia" uniqKey="Marquez Jurado S" first="Silvia" last="Márquez-Jurado">Silvia Márquez-Jurado</name>
</author>
<author><name sortKey="Nogales, Aitor" sort="Nogales, Aitor" uniqKey="Nogales A" first="Aitor" last="Nogales">Aitor Nogales</name>
</author>
<author><name sortKey="Enjuanes, Luis" sort="Enjuanes, Luis" uniqKey="Enjuanes L" first="Luis" last="Enjuanes">Luis Enjuanes</name>
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<term>Cricetinae</term>
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<front><div type="abstract" xml:lang="en">The large size of the coronavirus (CoV) genome (around 30 kb) and the instability in bacteria of plasmids carrying CoV replicase sequences represent serious restrictions for the development of CoV infectious clones using reverse genetic systems similar to those used for smaller positive sense RNA viruses. To overcome these problems, several approaches have been established in the last 13 years. Here we describe the engineering of CoV full-length cDNA clones as bacterial artificial chromosomes (BACs), using the Middle East respiratory syndrome CoV (MERS-CoV) as a model. </div>
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Engineering infectious cDNAs of coronavirus as bacterial artificial chromosomes.

Engineering infectious cDNAs of coronavirus as bacterial artificial chromosomes.

Source :

English descriptors

Abstract

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki