Engineering infectious cDNAs of coronavirus as bacterial artificial chromosomes.
Identifieur interne : 001687 ( PubMed/Corpus ); précédent : 001686; suivant : 001688Engineering infectious cDNAs of coronavirus as bacterial artificial chromosomes.
Auteurs : Fernando Almazán ; Silvia Márquez-Jurado ; Aitor Nogales ; Luis EnjuanesSource :
- Methods in molecular biology (Clifton, N.J.) [ 1940-6029 ] ; 2015.
English descriptors
- KwdEn :
- MESH :
- chemical , genetics : DNA, Complementary.
- genetics : Chromosomes, Artificial, Bacterial, Coronavirus, Plasmids.
- isolation & purification : Plasmids.
- Animals, Cell Line, Cricetinae, Escherichia coli, Genetic Engineering, Humans, Reverse Genetics, Transformation, Genetic.
Abstract
The large size of the coronavirus (CoV) genome (around 30 kb) and the instability in bacteria of plasmids carrying CoV replicase sequences represent serious restrictions for the development of CoV infectious clones using reverse genetic systems similar to those used for smaller positive sense RNA viruses. To overcome these problems, several approaches have been established in the last 13 years. Here we describe the engineering of CoV full-length cDNA clones as bacterial artificial chromosomes (BACs), using the Middle East respiratory syndrome CoV (MERS-CoV) as a model.
DOI: 10.1007/978-1-4939-2438-7_13
PubMed: 25720478
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pubmed:25720478Le document en format XML
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<author><name sortKey="Marquez Jurado, Silvia" sort="Marquez Jurado, Silvia" uniqKey="Marquez Jurado S" first="Silvia" last="Márquez-Jurado">Silvia Márquez-Jurado</name>
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<author><name sortKey="Nogales, Aitor" sort="Nogales, Aitor" uniqKey="Nogales A" first="Aitor" last="Nogales">Aitor Nogales</name>
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<term>Coronavirus (genetics)</term>
<term>Cricetinae</term>
<term>DNA, Complementary (genetics)</term>
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<front><div type="abstract" xml:lang="en">The large size of the coronavirus (CoV) genome (around 30 kb) and the instability in bacteria of plasmids carrying CoV replicase sequences represent serious restrictions for the development of CoV infectious clones using reverse genetic systems similar to those used for smaller positive sense RNA viruses. To overcome these problems, several approaches have been established in the last 13 years. Here we describe the engineering of CoV full-length cDNA clones as bacterial artificial chromosomes (BACs), using the Middle East respiratory syndrome CoV (MERS-CoV) as a model. </div>
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